MD05G1288800.v1.1

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0009324
Physical Location & Seq
Reverse (-)
42131114 .. 42131967
854 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1288800.v1.1.491

Sequence Viewer

Length: 732 bp
ATGCAGAGTGGCCAGCAGTTTGACCCCAACAAGGCAGCTCCGCCCTTTGCAAATCAAGTGGGTAATAATTACATGCACATTCCAGTTGCTTCTGCTTATGGGGCAGTTTTACCTAATGAGGCAAATCTCTTTAAACCTTTCCATGGTGTTGAATTTCAAACCTCTAGCATCTGCCCGAAGAATTTCATTATCTTCGACCAGACTGATCATCGAAGTCAGATTATGTTTAATCCTGAAATTGCCCACAAAATTACTGGTCCTGCCTTTAACCTCTGTGCAGCTTACATCCAAGACAATCTGGGATTGAACGAGGGAAATAATGACAATAGAGAAGCATCATCTACTCTGAAGGAGGACTCCGATGATATTGATGCATTGTTGAGCTTGGATGAGGAAGAGCTGGAAGATTATGATGAGGAAGAGGTCAGTACAGCACGAACTCATGGAAATTATGGGAGCTCATTCTCTGATTCTTGCTCCAGTAATGGTTTGAAAACCAAGAAGGAAAGGCTGCGTTCTTCACTTGAAAAGTCTTCTGGCATTGGTAGCAGCAGCAGCTGCAACAGTGAAAGGAAACGCCAGAAAATGAAGAAGATGGTGAGGGCGCTGAGAGGGATTGTACCTGGTGGGAATGAAATGAACACCGTCGCAGTTCTGGACGAAGCTGTTCAGTACTTGAAGTCTCTCAAGGTTGAGCTGCAGAAGATTGGAGTTGAGAATTTGAACGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

244

Amino Acids

26.79

Weight (kDa)

5.06

Isoelectric Point (pI)

51.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
bHLH_SAC51 PF23173 188 - 238 1e-17 Transcription factor SAC51 bHLH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 41
AcoI YGGCCR 1 cut(s) 10
AcsI RAATTY 3 cut(s) 152, 181, 718
AcuI CTGAAG 1 cut(s) 368
AfaI GTAC 3 cut(s) 430, 621, 674
AfiI CCNNNNNNNGG 2 cut(s) 31, 143
AgsI TTSAA 7 cut(s) 152, 158, 307, 493, 527, 679, 724
AjnI CCWGG 1 cut(s) 622
AluBI AGCT 8 cut(s) 38, 281, 384, 400, 459, 558, 665, 697
AluI AGCT 8 cut(s) 38, 281, 384, 400, 459, 558, 665, 697
Alw21I GWGCWC 1 cut(s) 461
Alw26I GTCTC 1 cut(s) 687
AlwNI CAGNNNCTG 1 cut(s) 558
AoxI GGCC 1 cut(s) 10
ApeKI GCWGC 8 cut(s) 35, 278, 511, 549, 552, 555, 558, 697
ApoI RAATTY 3 cut(s) 152, 181, 718
Asp700I GAANNNNTTC 2 cut(s) 182, 666
AspLEI GCGC 1 cut(s) 607
AspS9I GGNCC 1 cut(s) 257
AsuHPI GGTGA 1 cut(s) 610
AvaII GGWCC 1 cut(s) 257
BalI TGGCCA 1 cut(s) 12
BanII GRGCYC 1 cut(s) 461
BbsI GAAGAC 1 cut(s) 525
Bbv12I GWGCWC 1 cut(s) 461
BbvI GCAGC 8 cut(s) 47, 290, 498, 545, 561, 564, 567, 684
BccI CCATC 1 cut(s) 589
BciT130I CCWGG 1 cut(s) 624
BclI TGATCA 1 cut(s) 205
BcoDI GTCTC 1 cut(s) 687
BfaI CTAG 1 cut(s) 165
BfmI CTRYAG 1 cut(s) 698
BfoI RGCGCY 1 cut(s) 608
BisI GCNGC 8 cut(s) 36, 279, 512, 550, 553, 556, 559, 698
BlsI GCNGC 8 cut(s) 37, 280, 513, 551, 554, 557, 560, 699
BmcAI AGTACT 1 cut(s) 674
Bme1390I CCNGG 1 cut(s) 624
Bme18I GGWCC 1 cut(s) 257
BmgT120I GGNCC 1 cut(s) 257
BmrFI CCNGG 1 cut(s) 624
BmsI GCATC 3 cut(s) 177, 344, 361
BpiI GAAGAC 1 cut(s) 525
BpmI CTGGAG 1 cut(s) 463
BpuEI CTTGAG 1 cut(s) 671
BsaJI CCNNGG 1 cut(s) 142
Bsc4I CCNNNNNNNGG 2 cut(s) 31, 143
Bse1I ACTGG 3 cut(s) 83, 259, 480
BseBI CCWGG 1 cut(s) 624
BseDI CCNNGG 1 cut(s) 142
BseGI GGATG 2 cut(s) 285, 394
BseLI CCNNNNNNNGG 2 cut(s) 31, 143
BseMII CTCAG 1 cut(s) 599
BseNI ACTGG 3 cut(s) 83, 259, 480
BseXI GCAGC 8 cut(s) 47, 290, 498, 545, 561, 564, 567, 684
BsgI GTGCAG 1 cut(s) 297
BshFI GGCC 1 cut(s) 12
BsiHKAI GWGCWC 1 cut(s) 461
BslI CCNNNNNNNGG 2 cut(s) 31, 143
BsmAI GTCTC 1 cut(s) 687
BsnI GGCC 1 cut(s) 12
Bsp1286I GDGCHC 1 cut(s) 461
Bsp143I GATC 1 cut(s) 205
Bsp19I CCATGG 1 cut(s) 142
BspACI CCGC 1 cut(s) 41
BspANI GGCC 1 cut(s) 12
BspCNI CTCAG 1 cut(s) 600
BspMAI CTGCAG 1 cut(s) 702
BspQI GCTCTTC 1 cut(s) 390
BsrI ACTGG 3 cut(s) 83, 259, 480
BssECI CCNNGG 1 cut(s) 142
BssMI GATC 1 cut(s) 205
BssT1I CCWWGG 1 cut(s) 142
Bst2UI CCWGG 1 cut(s) 624
Bst4CI ACNGT 2 cut(s) 566, 646
Bst6I CTCTTC 2 cut(s) 390, 414
BstAPI GCANNNNNTGC 1 cut(s) 558
BstC8I GCNNGC 1 cut(s) 14
BstDEI CTNAG 1 cut(s) 608
BstDSI CCRYGG 1 cut(s) 142
BstF5I GGATG 2 cut(s) 285, 394
BstH2I RGCGCY 1 cut(s) 608
BstHHI GCGC 1 cut(s) 607
BstKTI GATC 1 cut(s) 208
BstMAI GTCTC 1 cut(s) 687
BstMBI GATC 1 cut(s) 205
BstMWI GCNNNNNNNGC 4 cut(s) 101, 546, 555, 558
BstNI CCWGG 1 cut(s) 624
BstNSI RCATGY 1 cut(s) 76
BstSCI CCNGG 1 cut(s) 622
BstSFI CTRYAG 1 cut(s) 698
BstV1I GCAGC 8 cut(s) 47, 290, 498, 545, 561, 564, 567, 684
BstV2I GAAGAC 1 cut(s) 525
BsuRI GGCC 1 cut(s) 12
BtgI CCRYGG 1 cut(s) 142
BtsCI GGATG 2 cut(s) 285, 394
BtsIMutI CAGTG 1 cut(s) 571
Cac8I GCNNGC 1 cut(s) 14
CaiI CAGNNNCTG 1 cut(s) 558
CfoI GCGC 1 cut(s) 607
Cfr13I GGNCC 1 cut(s) 257
CsiI ACCWGGT 1 cut(s) 622
Csp6I GTAC 3 cut(s) 429, 620, 673
CspCI CAANNNNNGTGG 2 cut(s) 39, 74
CviAII CATG 3 cut(s) 73, 143, 443
CviQI GTAC 3 cut(s) 429, 620, 673
DdeI CTNAG 1 cut(s) 608
DpnI GATC 1 cut(s) 207
DpnII GATC 1 cut(s) 205
DraI TTTAAA 1 cut(s) 133
EaeI YGGCCR 1 cut(s) 10
Eam1104I CTCTTC 2 cut(s) 390, 414
EarI CTCTTC 2 cut(s) 390, 414
EciI GGCGGA 1 cut(s) 30
Ecl136II GAGCTC 1 cut(s) 459
Eco130I CCWWGG 1 cut(s) 142
Eco24I GRGCYC 1 cut(s) 461
Eco47I GGWCC 1 cut(s) 257
Eco53kI GAGCTC 1 cut(s) 459
Eco57I CTGAAG 1 cut(s) 368
EcoICRI GAGCTC 1 cut(s) 459
EcoRII CCWGG 1 cut(s) 622
EcoT14I CCWWGG 1 cut(s) 142
EcoT22I ATGCAT 1 cut(s) 376
EcoT38I GRGCYC 1 cut(s) 461
ErhI CCWWGG 1 cut(s) 142
FaeI CATG 3 cut(s) 76, 146, 446
FaiI YATR 7 cut(s) 74, 99, 144, 224, 411, 444, 453
FatI CATG 3 cut(s) 72, 142, 442
FbaI TGATCA 1 cut(s) 205
Fnu4HI GCNGC 8 cut(s) 36, 279, 512, 550, 553, 556, 559, 698
FokI GGATG 2 cut(s) 272, 401
FriOI GRGCYC 1 cut(s) 461
Fsp4HI GCNGC 8 cut(s) 36, 279, 512, 550, 553, 556, 559, 698
FspBI CTAG 1 cut(s) 165
GlaI GCGC 1 cut(s) 606
GluI GCNGC 8 cut(s) 36, 279, 512, 550, 553, 556, 559, 698
GsuI CTGGAG 1 cut(s) 463
HaeII RGCGCY 1 cut(s) 608
HaeIII GGCC 1 cut(s) 12
HhaI GCGC 1 cut(s) 607
Hin1II CATG 3 cut(s) 76, 146, 446
Hin6I GCGC 1 cut(s) 605
HinP1I GCGC 1 cut(s) 605
HinfI GANTC 2 cut(s) 356, 470
HphI GGTGA 1 cut(s) 610
Hpy188I TCNGA 4 cut(s) 219, 348, 361, 469
Hpy188III TCNNGA 2 cut(s) 233, 656
Hpy99I CGWCG 1 cut(s) 650
HpyAV CCTTC 2 cut(s) 343, 496
HpyCH4III ACNGT 2 cut(s) 566, 646
HpyCH4V TGCA 7 cut(s) 4, 50, 76, 278, 374, 561, 700
HpyF10VI GCNNNNNNNGC 4 cut(s) 101, 546, 555, 558
HpyF3I CTNAG 1 cut(s) 608
Hsp92II CATG 3 cut(s) 76, 146, 446
HspAI GCGC 1 cut(s) 605
Ksp22I TGATCA 1 cut(s) 205
Kzo9I GATC 1 cut(s) 205
LguI GCTCTTC 1 cut(s) 390
LmnI GCTCC 3 cut(s) 43, 456, 482
Lsp1109I GCAGC 8 cut(s) 47, 290, 498, 545, 561, 564, 567, 684
LweI GCATC 3 cut(s) 177, 344, 361
MabI ACCWGGT 1 cut(s) 622
MaeI CTAG 1 cut(s) 165
MalI GATC 1 cut(s) 207
MboI GATC 1 cut(s) 205
MhlI GDGCHC 1 cut(s) 461
MlsI TGGCCA 1 cut(s) 12
MluCI AATT 7 cut(s) 67, 152, 181, 237, 249, 448, 718
MluNI TGGCCA 1 cut(s) 12
MlyI GAGTC 1 cut(s) 350
Mox20I TGGCCA 1 cut(s) 12
Mph1103I ATGCAT 1 cut(s) 376
MroXI GAANNNNTTC 2 cut(s) 182, 666
MscI TGGCCA 1 cut(s) 12
MseI TTAA 3 cut(s) 132, 228, 267
Msp20I TGGCCA 1 cut(s) 12
MspA1I CMGCKG 1 cut(s) 558
MspR9I CCNGG 1 cut(s) 624
MvaI CCWGG 1 cut(s) 624
MwoI GCNNNNNNNGC 4 cut(s) 101, 546, 555, 558
NcoI CCATGG 1 cut(s) 142
NdeII GATC 1 cut(s) 205
NlaIII CATG 3 cut(s) 76, 146, 446
NsiI ATGCAT 1 cut(s) 376
NspI RCATGY 1 cut(s) 76
PciSI GCTCTTC 1 cut(s) 390
PdmI GAANNNNTTC 2 cut(s) 182, 666
PfeI GAWTC 1 cut(s) 470
PkrI GCNGC 8 cut(s) 37, 280, 513, 551, 554, 557, 560, 699
PleI GAGTC 1 cut(s) 350
PpsI GAGTC 1 cut(s) 350
Psp124BI GAGCTC 1 cut(s) 461
Psp6I CCWGG 1 cut(s) 622
PspGI CCWGG 1 cut(s) 622
PspPI GGNCC 1 cut(s) 257
PstI CTGCAG 1 cut(s) 702
PstNI CAGNNNCTG 1 cut(s) 558
PvuII CAGCTG 1 cut(s) 558
RsaI GTAC 3 cut(s) 430, 621, 674
RsaNI GTAC 3 cut(s) 429, 620, 673
SacI GAGCTC 1 cut(s) 461
SapI GCTCTTC 1 cut(s) 390
SaqAI TTAA 3 cut(s) 132, 228, 267
SatI GCNGC 8 cut(s) 36, 279, 512, 550, 553, 556, 559, 698
Sau3AI GATC 1 cut(s) 205
Sau96I GGNCC 1 cut(s) 257
ScaI AGTACT 1 cut(s) 674
SchI GAGTC 1 cut(s) 350
ScrFI CCNGG 1 cut(s) 624
SduI GDGCHC 1 cut(s) 461
SexAI ACCWGGT 1 cut(s) 622
SfaNI GCATC 3 cut(s) 177, 344, 361
SfcI CTRYAG 1 cut(s) 698
SinI GGWCC 1 cut(s) 257
SmlI CTYRAG 1 cut(s) 686
SmoI CTYRAG 1 cut(s) 686
Sse9I AATT 7 cut(s) 67, 152, 181, 237, 249, 448, 718
SsiI CCGC 1 cut(s) 41
SspMI CTAG 1 cut(s) 165
SstI GAGCTC 1 cut(s) 461
StyD4I CCNGG 1 cut(s) 622
StyI CCWWGG 1 cut(s) 142
TaaI ACNGT 2 cut(s) 566, 646
TaqI TCGA 2 cut(s) 195, 211
TasI AATT 7 cut(s) 67, 152, 181, 237, 249, 448, 718
TatI WGTACW 2 cut(s) 428, 672
TfiI GAWTC 1 cut(s) 470
Tru1I TTAA 3 cut(s) 132, 228, 267
Tru9I TTAA 3 cut(s) 132, 228, 267
TscAI CASTG 1 cut(s) 571
TseI GCWGC 8 cut(s) 35, 278, 511, 549, 552, 555, 558, 697
TspDTI ATGAA 4 cut(s) 175, 602, 648, 653
TspRI CASTG 1 cut(s) 571
VpaK11BI GGWCC 1 cut(s) 257
XapI RAATTY 3 cut(s) 152, 181, 718
XceI RCATGY 1 cut(s) 76
XcmI CCANNNNNNNNNTGG 1 cut(s) 251
XmnI GAANNNNTTC 2 cut(s) 182, 666
XspI CTAG 1 cut(s) 165
ZrmI AGTACT 1 cut(s) 674
Zsp2I ATGCAT 1 cut(s) 376
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.