pycom05g26630

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0009324
Physical Location & Seq
Reverse (-)
27811951 .. 27812947
997 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g26630.1

Sequence Viewer

Length: 804 bp
ATGCAGAGTGACCAGCAGTTTGACCCTAACAAGGCAGCTCCGCCCTTTGCAAATCAAGTGGGTAATAATTACATGCACATTCCGGTTGCTTCTGCGTGTGGGGCAGTTTTACCTAATGAGGCAAATCGCTTTAAACCTTTCCATGGTGTTGAATTTCAAACCTCTAGCATCTGCCCAAAGAATTTTATTATCTTCGACCAGACTGATCATCGAAGTCAGATTATGTTCAATCCTGAAATTGCCCACAAAATTACTGGTCCTGCCTTTAACCTCTGTGCAGCTTACATCCAAGACAATCTGGGATTGAACGAGAGAAATAATGACAATAGAGAAGCATCGTCTACTCTGAAGGAGGACTCCGATGATATTGATGCATTGTTGAGCTTGGATGAGGAAGAGCTGGAAGATTATGATGAGGAAGAGGTCAGTACAGCACGAACTCATGGAAATTACGGGAGCTCATTCTCTGATTCTTGCTCCAGTTATGGTTTGAAAACCAAGAAGGAAGGGCTGCGTTCTTCACTTGAAAAGTCTTCTGGCATTGGTAGCAGCGGCAGCAGTTACAACAGTGAAAGGAAACGCCAGAAAATGAAGAAGATGGTGAGGGCGCTGAGAGGGATTGTACCTGGTGGGAAAGAAATGAACACCGTCGCCGTTCTGGACGAAGCTGTTCAGTACTTGAAGTCTCTCAAGTGTATTCCAGTCCTCTCTTTGATTTGTATTATGATTTGTAGTATGGATGCTCATTTTCTTGTATTTCACTTAATTTCGTATGGCAAGTTGCAGCTATCGACTGCTATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

268

Amino Acids

29.55

Weight (kDa)

5.53

Isoelectric Point (pI)

48.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
bHLH_SAC51 PF23173 188 - 231 4.4e-17 Transcription factor SAC51 bHLH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 341
AciI CCGC 2 cut(s) 41, 552
AcsI RAATTY 2 cut(s) 152, 181
AcuI CTGAAG 1 cut(s) 368
AfaI GTAC 3 cut(s) 430, 624, 677
AfiI CCNNNNNNNGG 2 cut(s) 31, 143
AgsI TTSAA 7 cut(s) 152, 158, 229, 307, 493, 527, 682
AjnI CCWGG 1 cut(s) 625
AluBI AGCT 7 cut(s) 38, 281, 384, 400, 459, 668, 787
AluI AGCT 7 cut(s) 38, 281, 384, 400, 459, 668, 787
Alw21I GWGCWC 1 cut(s) 461
Alw26I GTCTC 1 cut(s) 690
ApeKI GCWGC 6 cut(s) 35, 278, 511, 549, 555, 784
ApoI RAATTY 2 cut(s) 152, 181
ArsI GACNNNNNNTTYG 1 cut(s) 34
Asp700I GAANNNNTTC 1 cut(s) 669
AspLEI GCGC 1 cut(s) 610
AspS9I GGNCC 1 cut(s) 257
AsuHPI GGTGA 1 cut(s) 613
AvaII GGWCC 1 cut(s) 257
BanII GRGCYC 1 cut(s) 461
BbsI GAAGAC 1 cut(s) 525
Bbv12I GWGCWC 1 cut(s) 461
BbvI GCAGC 6 cut(s) 47, 290, 498, 561, 567, 796
BccI CCATC 1 cut(s) 592
BceAI ACGGC 1 cut(s) 638
BciT130I CCWGG 1 cut(s) 627
BclI TGATCA 1 cut(s) 205
BcoDI GTCTC 1 cut(s) 690
BfaI CTAG 1 cut(s) 165
BfoI RGCGCY 1 cut(s) 611
BisI GCNGC 7 cut(s) 36, 279, 512, 550, 553, 556, 785
BlsI GCNGC 7 cut(s) 37, 280, 513, 551, 554, 557, 786
BmcAI AGTACT 1 cut(s) 677
Bme1390I CCNGG 1 cut(s) 627
Bme18I GGWCC 1 cut(s) 257
BmgT120I GGNCC 1 cut(s) 257
BmrFI CCNGG 1 cut(s) 627
BmsI GCATC 4 cut(s) 177, 344, 361, 730
BpiI GAAGAC 1 cut(s) 525
BpmI CTGGAG 1 cut(s) 463
BpuEI CTTGAG 1 cut(s) 674
BsaJI CCNNGG 1 cut(s) 142
BsaWI WCCGGW 1 cut(s) 82
Bsc4I CCNNNNNNNGG 2 cut(s) 31, 143
Bse1I ACTGG 3 cut(s) 259, 480, 701
BseBI CCWGG 1 cut(s) 627
BseDI CCNNGG 1 cut(s) 142
BseGI GGATG 3 cut(s) 285, 394, 745
BseLI CCNNNNNNNGG 2 cut(s) 31, 143
BseMII CTCAG 1 cut(s) 602
BseNI ACTGG 3 cut(s) 259, 480, 701
BseXI GCAGC 6 cut(s) 47, 290, 498, 561, 567, 796
BsgI GTGCAG 1 cut(s) 297
BsiHKAI GWGCWC 1 cut(s) 461
BsiSI CCGG 1 cut(s) 83
BslI CCNNNNNNNGG 2 cut(s) 31, 143
BsmAI GTCTC 1 cut(s) 690
Bsp1286I GDGCHC 1 cut(s) 461
Bsp143I GATC 1 cut(s) 205
Bsp19I CCATGG 1 cut(s) 142
BspACI CCGC 2 cut(s) 41, 552
BspCNI CTCAG 1 cut(s) 603
BspQI GCTCTTC 1 cut(s) 390
BsrI ACTGG 3 cut(s) 259, 480, 701
BssECI CCNNGG 1 cut(s) 142
BssMI GATC 1 cut(s) 205
BssT1I CCWWGG 1 cut(s) 142
Bst2UI CCWGG 1 cut(s) 627
Bst4CI ACNGT 2 cut(s) 569, 649
Bst6I CTCTTC 2 cut(s) 390, 414
BstDEI CTNAG 1 cut(s) 611
BstDSI CCRYGG 1 cut(s) 142
BstF5I GGATG 3 cut(s) 285, 394, 745
BstH2I RGCGCY 1 cut(s) 611
BstHHI GCGC 1 cut(s) 610
BstKTI GATC 1 cut(s) 208
BstMAI GTCTC 1 cut(s) 690
BstMBI GATC 1 cut(s) 205
BstMWI GCNNNNNNNGC 3 cut(s) 101, 546, 555
BstNI CCWGG 1 cut(s) 627
BstNSI RCATGY 1 cut(s) 76
BstSCI CCNGG 1 cut(s) 625
BstV1I GCAGC 6 cut(s) 47, 290, 498, 561, 567, 796
BstV2I GAAGAC 1 cut(s) 525
BtgI CCRYGG 1 cut(s) 142
BtsCI GGATG 3 cut(s) 285, 394, 745
BtsIMutI CAGTG 1 cut(s) 574
CfoI GCGC 1 cut(s) 610
Cfr13I GGNCC 1 cut(s) 257
CsiI ACCWGGT 1 cut(s) 625
Csp6I GTAC 3 cut(s) 429, 623, 676
CspCI CAANNNNNGTGG 2 cut(s) 39, 74
CviAII CATG 3 cut(s) 73, 143, 443
CviJI RGCY 8 cut(s) 38, 281, 384, 400, 459, 511, 668, 787
CviKI_1 RGCY 8 cut(s) 38, 281, 384, 400, 459, 511, 668, 787
CviQI GTAC 3 cut(s) 429, 623, 676
DdeI CTNAG 1 cut(s) 611
DpnI GATC 1 cut(s) 207
DpnII GATC 1 cut(s) 205
DraI TTTAAA 1 cut(s) 133
Eam1104I CTCTTC 2 cut(s) 390, 414
EarI CTCTTC 2 cut(s) 390, 414
EciI GGCGGA 1 cut(s) 30
Ecl136II GAGCTC 1 cut(s) 459
Eco130I CCWWGG 1 cut(s) 142
Eco24I GRGCYC 1 cut(s) 461
Eco47I GGWCC 1 cut(s) 257
Eco53kI GAGCTC 1 cut(s) 459
Eco57I CTGAAG 1 cut(s) 368
EcoICRI GAGCTC 1 cut(s) 459
EcoRII CCWGG 1 cut(s) 625
EcoT14I CCWWGG 1 cut(s) 142
EcoT22I ATGCAT 1 cut(s) 376
EcoT38I GRGCYC 1 cut(s) 461
ErhI CCWWGG 1 cut(s) 142
FaeI CATG 3 cut(s) 76, 146, 446
FatI CATG 3 cut(s) 72, 142, 442
FbaI TGATCA 1 cut(s) 205
FblI GTMKAC 1 cut(s) 341
Fnu4HI GCNGC 7 cut(s) 36, 279, 512, 550, 553, 556, 785
FokI GGATG 3 cut(s) 272, 401, 752
FriOI GRGCYC 1 cut(s) 461
Fsp4HI GCNGC 7 cut(s) 36, 279, 512, 550, 553, 556, 785
FspBI CTAG 1 cut(s) 165
GlaI GCGC 1 cut(s) 609
GluI GCNGC 7 cut(s) 36, 279, 512, 550, 553, 556, 785
GsuI CTGGAG 1 cut(s) 463
HaeII RGCGCY 1 cut(s) 611
HapII CCGG 1 cut(s) 83
HhaI GCGC 1 cut(s) 610
Hin1II CATG 3 cut(s) 76, 146, 446
Hin6I GCGC 1 cut(s) 608
HinP1I GCGC 1 cut(s) 608
HinfI GANTC 2 cut(s) 356, 470
HpaII CCGG 1 cut(s) 83
HphI GGTGA 1 cut(s) 613
Hpy166II GTNNAC 1 cut(s) 342
Hpy188I TCNGA 4 cut(s) 219, 348, 361, 469
Hpy188III TCNNGA 2 cut(s) 233, 659
Hpy8I GTNNAC 1 cut(s) 342
Hpy99I CGWCG 1 cut(s) 653
HpyAV CCTTC 3 cut(s) 343, 496, 500
HpyCH4III ACNGT 2 cut(s) 569, 649
HpyCH4V TGCA 6 cut(s) 4, 50, 76, 278, 374, 784
HpyF10VI GCNNNNNNNGC 3 cut(s) 101, 546, 555
HpyF3I CTNAG 1 cut(s) 611
Hsp92II CATG 3 cut(s) 76, 146, 446
HspAI GCGC 1 cut(s) 608
Ksp22I TGATCA 1 cut(s) 205
Kzo9I GATC 1 cut(s) 205
LguI GCTCTTC 1 cut(s) 390
LmnI GCTCC 3 cut(s) 43, 456, 482
Lsp1109I GCAGC 6 cut(s) 47, 290, 498, 561, 567, 796
LweI GCATC 4 cut(s) 177, 344, 361, 730
MabI ACCWGGT 1 cut(s) 625
MaeI CTAG 1 cut(s) 165
MaeIII GTNAC 2 cut(s) 8, 560
MalI GATC 1 cut(s) 207
MboI GATC 1 cut(s) 205
MboII GAAGA 8 cut(s) 184, 407, 416, 431, 510, 525, 604, 607
MhlI GDGCHC 1 cut(s) 461
MluCI AATT 7 cut(s) 67, 152, 181, 237, 249, 448, 765
MlyI GAGTC 1 cut(s) 350
Mph1103I ATGCAT 1 cut(s) 376
MroXI GAANNNNTTC 1 cut(s) 669
MseI TTAA 3 cut(s) 132, 267, 764
MspA1I CMGCKG 1 cut(s) 552
MspI CCGG 1 cut(s) 83
MspR9I CCNGG 1 cut(s) 627
MvaI CCWGG 1 cut(s) 627
MwoI GCNNNNNNNGC 3 cut(s) 101, 546, 555
NcoI CCATGG 1 cut(s) 142
NdeII GATC 1 cut(s) 205
NlaIII CATG 3 cut(s) 76, 146, 446
NmuCI GTSAC 1 cut(s) 8
NsiI ATGCAT 1 cut(s) 376
NspI RCATGY 1 cut(s) 76
PciSI GCTCTTC 1 cut(s) 390
PdmI GAANNNNTTC 1 cut(s) 669
PfeI GAWTC 1 cut(s) 470
PkrI GCNGC 7 cut(s) 37, 280, 513, 551, 554, 557, 786
PleI GAGTC 1 cut(s) 350
PpsI GAGTC 1 cut(s) 350
Psp124BI GAGCTC 1 cut(s) 461
Psp6I CCWGG 1 cut(s) 625
PspGI CCWGG 1 cut(s) 625
PspPI GGNCC 1 cut(s) 257
RsaI GTAC 3 cut(s) 430, 624, 677
RsaNI GTAC 3 cut(s) 429, 623, 676
SacI GAGCTC 1 cut(s) 461
SapI GCTCTTC 1 cut(s) 390
SaqAI TTAA 3 cut(s) 132, 267, 764
SatI GCNGC 7 cut(s) 36, 279, 512, 550, 553, 556, 785
Sau3AI GATC 1 cut(s) 205
Sau96I GGNCC 1 cut(s) 257
ScaI AGTACT 1 cut(s) 677
SchI GAGTC 1 cut(s) 350
ScrFI CCNGG 1 cut(s) 627
SduI GDGCHC 1 cut(s) 461
SexAI ACCWGGT 1 cut(s) 625
SfaNI GCATC 4 cut(s) 177, 344, 361, 730
SinI GGWCC 1 cut(s) 257
SmlI CTYRAG 1 cut(s) 689
SmoI CTYRAG 1 cut(s) 689
Sse9I AATT 7 cut(s) 67, 152, 181, 237, 249, 448, 765
SsiI CCGC 2 cut(s) 41, 552
SspMI CTAG 1 cut(s) 165
SstI GAGCTC 1 cut(s) 461
StyD4I CCNGG 1 cut(s) 625
StyI CCWWGG 1 cut(s) 142
TaaI ACNGT 2 cut(s) 569, 649
TaqI TCGA 3 cut(s) 195, 211, 791
TasI AATT 7 cut(s) 67, 152, 181, 237, 249, 448, 765
TatI WGTACW 2 cut(s) 428, 675
TauI GCSGC 1 cut(s) 555
TfiI GAWTC 1 cut(s) 470
Tru1I TTAA 3 cut(s) 132, 267, 764
Tru9I TTAA 3 cut(s) 132, 267, 764
TscAI CASTG 1 cut(s) 574
TseFI GTSAC 1 cut(s) 8
TseI GCWGC 6 cut(s) 35, 278, 511, 549, 555, 784
Tsp45I GTSAC 1 cut(s) 8
TspDTI ATGAA 2 cut(s) 605, 656
TspRI CASTG 1 cut(s) 574
VpaK11BI GGWCC 1 cut(s) 257
XapI RAATTY 2 cut(s) 152, 181
XceI RCATGY 1 cut(s) 76
XcmI CCANNNNNNNNNTGG 1 cut(s) 251
XmiI GTMKAC 1 cut(s) 341
XmnI GAANNNNTTC 1 cut(s) 669
XspI CTAG 1 cut(s) 165
ZrmI AGTACT 1 cut(s) 677
Zsp2I ATGCAT 1 cut(s) 376
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.