MD05G1288000.v1.1

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0009324
Physical Location & Seq
Reverse (-)
42055526 .. 42056184
659 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1288000.v1.1.491

Sequence Viewer

Length: 600 bp
ATGCACATTCCAGTTGCTTCTGCGTATGGGGCAGTTTTACCTAATGAGGCAAATCACTTTAAACCTTTCCATGGTGTTGAATTTCAAACCTCTAGCATCTGCCCAAAGAATTTCATTATCTTCGACCAGACTGATCATCGAAGTCAGATTATGTTCAATCCTGAAATTGCCCACAAAATTACTGGTCCTGCCTTTAACCTCTGTGCAGCTTACATCCAAGACAATCTGGAATTGAACGAGGGAAATAATGACAATAGAGAAGCATCGTCTACTCTGAAGGAGGATTCCGATGATATTGATGCATTGTTGAGCTTGGTGAGGAAGAGCTGGAAGATTATGATGAGGAAGAGTTATGGTTTGAAAACCAAGAAGGAAAGGCTGCGTTCTTCACTTGAAAAGTCTTCCGGCGTTGGTAGCAGCAGCAGCTGCAACAGTGAAAGGAAACGCCAGAAAACGAAGAAGATGGTGAGGGCGCTGAGAGGGATTGTACCTGGTGGGAATGAAACGAACACCGTTGCTGTTCTGGACGAAGCTGTTCAGTACTTGAAGTCTCTCAAGGTTGAGCTGCAGAAGATTGGAATTGAGAATTTGAACGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

200

Amino Acids

22.24

Weight (kDa)

8.91

Isoelectric Point (pI)

38.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
bHLH_SAC51 PF23173 144 - 191 5.9e-16 Transcription factor SAC51 bHLH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 269
AcsI RAATTY 3 cut(s) 80, 109, 586
AcuI CTGAAG 1 cut(s) 296
AfaI GTAC 2 cut(s) 489, 542
AfiI CCNNNNNNNGG 1 cut(s) 71
AgsI TTSAA 8 cut(s) 80, 86, 157, 235, 361, 395, 547, 592
AjnI CCWGG 1 cut(s) 490
AluBI AGCT 6 cut(s) 209, 312, 327, 426, 533, 565
AluI AGCT 6 cut(s) 209, 312, 327, 426, 533, 565
Alw26I GTCTC 1 cut(s) 555
AlwNI CAGNNNCTG 1 cut(s) 426
ApeKI GCWGC 7 cut(s) 206, 379, 417, 420, 423, 426, 565
ApoI RAATTY 3 cut(s) 80, 109, 586
Asp700I GAANNNNTTC 1 cut(s) 534
AspLEI GCGC 1 cut(s) 475
AspS9I GGNCC 1 cut(s) 185
AsuHPI GGTGA 2 cut(s) 328, 478
AvaII GGWCC 1 cut(s) 185
BbsI GAAGAC 1 cut(s) 393
BbvI GCAGC 7 cut(s) 218, 366, 413, 429, 432, 435, 552
BccI CCATC 1 cut(s) 457
BciT130I CCWGG 1 cut(s) 492
BclI TGATCA 1 cut(s) 133
BcoDI GTCTC 1 cut(s) 555
BfaI CTAG 1 cut(s) 93
BfmI CTRYAG 1 cut(s) 566
BfoI RGCGCY 1 cut(s) 476
BisI GCNGC 7 cut(s) 207, 380, 418, 421, 424, 427, 566
BlsI GCNGC 7 cut(s) 208, 381, 419, 422, 425, 428, 567
BmcAI AGTACT 1 cut(s) 542
Bme1390I CCNGG 1 cut(s) 492
Bme18I GGWCC 1 cut(s) 185
BmgT120I GGNCC 1 cut(s) 185
BmrFI CCNGG 1 cut(s) 492
BmsI GCATC 3 cut(s) 105, 272, 289
BpiI GAAGAC 1 cut(s) 393
BpuEI CTTGAG 1 cut(s) 539
BsaJI CCNNGG 1 cut(s) 70
Bsc4I CCNNNNNNNGG 1 cut(s) 71
Bse1I ACTGG 2 cut(s) 11, 187
BseBI CCWGG 1 cut(s) 492
BseDI CCNNGG 1 cut(s) 70
BseGI GGATG 1 cut(s) 213
BseLI CCNNNNNNNGG 1 cut(s) 71
BseMII CTCAG 1 cut(s) 467
BseNI ACTGG 2 cut(s) 11, 187
BseXI GCAGC 7 cut(s) 218, 366, 413, 429, 432, 435, 552
BsgI GTGCAG 1 cut(s) 225
BsiSI CCGG 1 cut(s) 405
BslI CCNNNNNNNGG 1 cut(s) 71
BsmAI GTCTC 1 cut(s) 555
Bsp143I GATC 1 cut(s) 133
Bsp19I CCATGG 1 cut(s) 70
BspCNI CTCAG 1 cut(s) 468
BspMAI CTGCAG 1 cut(s) 570
BspQI GCTCTTC 1 cut(s) 317
BsrI ACTGG 2 cut(s) 11, 187
BssECI CCNNGG 1 cut(s) 70
BssMI GATC 1 cut(s) 133
BssT1I CCWWGG 1 cut(s) 70
Bst2UI CCWGG 1 cut(s) 492
Bst4CI ACNGT 2 cut(s) 434, 514
Bst6I CTCTTC 2 cut(s) 317, 341
BstAPI GCANNNNNTGC 1 cut(s) 426
BstDEI CTNAG 1 cut(s) 476
BstDSI CCRYGG 1 cut(s) 70
BstF5I GGATG 1 cut(s) 213
BstH2I RGCGCY 1 cut(s) 476
BstHHI GCGC 1 cut(s) 475
BstKTI GATC 1 cut(s) 136
BstMAI GTCTC 1 cut(s) 555
BstMBI GATC 1 cut(s) 133
BstMWI GCNNNNNNNGC 4 cut(s) 29, 414, 423, 426
BstNI CCWGG 1 cut(s) 492
BstSCI CCNGG 1 cut(s) 490
BstSFI CTRYAG 1 cut(s) 566
BstV1I GCAGC 7 cut(s) 218, 366, 413, 429, 432, 435, 552
BstV2I GAAGAC 1 cut(s) 393
BtgI CCRYGG 1 cut(s) 70
BtsCI GGATG 1 cut(s) 213
BtsIMutI CAGTG 1 cut(s) 439
CaiI CAGNNNCTG 1 cut(s) 426
CfoI GCGC 1 cut(s) 475
Cfr13I GGNCC 1 cut(s) 185
CsiI ACCWGGT 1 cut(s) 490
Csp6I GTAC 2 cut(s) 488, 541
CviAII CATG 1 cut(s) 71
CviJI RGCY 7 cut(s) 209, 312, 327, 379, 426, 533, 565
CviKI_1 RGCY 7 cut(s) 209, 312, 327, 379, 426, 533, 565
CviQI GTAC 2 cut(s) 488, 541
DdeI CTNAG 1 cut(s) 476
DpnI GATC 1 cut(s) 135
DpnII GATC 1 cut(s) 133
DraI TTTAAA 1 cut(s) 61
Eam1104I CTCTTC 2 cut(s) 317, 341
EarI CTCTTC 2 cut(s) 317, 341
Eco130I CCWWGG 1 cut(s) 70
Eco47I GGWCC 1 cut(s) 185
Eco57I CTGAAG 1 cut(s) 296
EcoRII CCWGG 1 cut(s) 490
EcoT14I CCWWGG 1 cut(s) 70
EcoT22I ATGCAT 1 cut(s) 304
ErhI CCWWGG 1 cut(s) 70
FaeI CATG 1 cut(s) 74
FaiI YATR 5 cut(s) 27, 72, 152, 338, 354
FatI CATG 1 cut(s) 70
FbaI TGATCA 1 cut(s) 133
FblI GTMKAC 1 cut(s) 269
Fnu4HI GCNGC 7 cut(s) 207, 380, 418, 421, 424, 427, 566
FokI GGATG 1 cut(s) 200
Fsp4HI GCNGC 7 cut(s) 207, 380, 418, 421, 424, 427, 566
FspBI CTAG 1 cut(s) 93
GlaI GCGC 1 cut(s) 474
GluI GCNGC 7 cut(s) 207, 380, 418, 421, 424, 427, 566
HaeII RGCGCY 1 cut(s) 476
HapII CCGG 1 cut(s) 405
HhaI GCGC 1 cut(s) 475
Hin1II CATG 1 cut(s) 74
Hin6I GCGC 1 cut(s) 473
HinP1I GCGC 1 cut(s) 473
HinfI GANTC 1 cut(s) 284
HpaII CCGG 1 cut(s) 405
HphI GGTGA 2 cut(s) 328, 478
Hpy166II GTNNAC 1 cut(s) 270
Hpy188I TCNGA 3 cut(s) 147, 276, 289
Hpy188III TCNNGA 3 cut(s) 161, 227, 524
Hpy8I GTNNAC 1 cut(s) 270
HpyAV CCTTC 2 cut(s) 271, 364
HpyCH4III ACNGT 2 cut(s) 434, 514
HpyCH4V TGCA 5 cut(s) 4, 206, 302, 429, 568
HpyF10VI GCNNNNNNNGC 4 cut(s) 29, 414, 423, 426
HpyF3I CTNAG 1 cut(s) 476
Hsp92II CATG 1 cut(s) 74
HspAI GCGC 1 cut(s) 473
Ksp22I TGATCA 1 cut(s) 133
Kzo9I GATC 1 cut(s) 133
LguI GCTCTTC 1 cut(s) 317
Lsp1109I GCAGC 7 cut(s) 218, 366, 413, 429, 432, 435, 552
LweI GCATC 3 cut(s) 105, 272, 289
MabI ACCWGGT 1 cut(s) 490
MaeI CTAG 1 cut(s) 93
MalI GATC 1 cut(s) 135
MboI GATC 1 cut(s) 133
MboII GAAGA 9 cut(s) 112, 334, 343, 358, 378, 393, 469, 472, 583
MluCI AATT 7 cut(s) 80, 109, 165, 177, 230, 579, 586
MnlI CCTC 9 cut(s) 40, 100, 209, 232, 274, 312, 336, 462, 473
Mph1103I ATGCAT 1 cut(s) 304
MroXI GAANNNNTTC 1 cut(s) 534
MseI TTAA 2 cut(s) 60, 195
MspA1I CMGCKG 1 cut(s) 426
MspI CCGG 1 cut(s) 405
MspR9I CCNGG 1 cut(s) 492
MvaI CCWGG 1 cut(s) 492
MwoI GCNNNNNNNGC 4 cut(s) 29, 414, 423, 426
NcoI CCATGG 1 cut(s) 70
NdeII GATC 1 cut(s) 133
NlaIII CATG 1 cut(s) 74
NsiI ATGCAT 1 cut(s) 304
PciSI GCTCTTC 1 cut(s) 317
PdmI GAANNNNTTC 1 cut(s) 534
PfeI GAWTC 1 cut(s) 284
PkrI GCNGC 7 cut(s) 208, 381, 419, 422, 425, 428, 567
Psp6I CCWGG 1 cut(s) 490
PspGI CCWGG 1 cut(s) 490
PspPI GGNCC 1 cut(s) 185
PstI CTGCAG 1 cut(s) 570
PstNI CAGNNNCTG 1 cut(s) 426
PvuII CAGCTG 1 cut(s) 426
RsaI GTAC 2 cut(s) 489, 542
RsaNI GTAC 2 cut(s) 488, 541
SapI GCTCTTC 1 cut(s) 317
SaqAI TTAA 2 cut(s) 60, 195
SatI GCNGC 7 cut(s) 207, 380, 418, 421, 424, 427, 566
Sau3AI GATC 1 cut(s) 133
Sau96I GGNCC 1 cut(s) 185
ScaI AGTACT 1 cut(s) 542
ScrFI CCNGG 1 cut(s) 492
SexAI ACCWGGT 1 cut(s) 490
SfaNI GCATC 3 cut(s) 105, 272, 289
SfcI CTRYAG 1 cut(s) 566
SinI GGWCC 1 cut(s) 185
SmlI CTYRAG 1 cut(s) 554
SmoI CTYRAG 1 cut(s) 554
Sse9I AATT 7 cut(s) 80, 109, 165, 177, 230, 579, 586
SspMI CTAG 1 cut(s) 93
StyD4I CCNGG 1 cut(s) 490
StyI CCWWGG 1 cut(s) 70
TaaI ACNGT 2 cut(s) 434, 514
TaqI TCGA 2 cut(s) 123, 139
TasI AATT 7 cut(s) 80, 109, 165, 177, 230, 579, 586
TatI WGTACW 1 cut(s) 540
TfiI GAWTC 1 cut(s) 284
Tru1I TTAA 2 cut(s) 60, 195
Tru9I TTAA 2 cut(s) 60, 195
TscAI CASTG 1 cut(s) 439
TseI GCWGC 7 cut(s) 206, 379, 417, 420, 423, 426, 565
TspDTI ATGAA 2 cut(s) 103, 516
TspRI CASTG 1 cut(s) 439
VpaK11BI GGWCC 1 cut(s) 185
XapI RAATTY 3 cut(s) 80, 109, 586
XcmI CCANNNNNNNNNTGG 1 cut(s) 179
XmiI GTMKAC 1 cut(s) 269
XmnI GAANNNNTTC 1 cut(s) 534
XspI CTAG 1 cut(s) 93
ZrmI AGTACT 1 cut(s) 542
Zsp2I ATGCAT 1 cut(s) 304
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.