MD05G1288500.v1.1

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0009324
Physical Location & Seq
Reverse (-)
42113744 .. 42115039
1296 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1288500.v1.1.491

Sequence Viewer

Length: 501 bp
ATGTGGCAAATCACTTTAAACCTTTCCATGGTGTTGAATTTCAAACCTTTAGCATCTGCTCGAAGAATTTCATTATCTTCGACCAGACTGATCATCGAAGTTAGGTTATGTTCAATCCTGAAATTGCCCACAAAATTACTGGTCCTGCCTTTAACCTCTGTGCAGCTTACATCCAAGACAATCTGGGAGCTGGAAGATTATGATGAGGAAGAGGTCAGTACAGCACGAACTCATGGAAATTATGGGAACTCATTCTCTGATTCTTGCTCCAGTTATGGTTTGAAAACCAAGAAGGAAAGGCTGCGTTCTTCACTTGAAAAGTCTTCTGGCGTTGGTAGCAGCAGCAGCTGCAACAGTGAAAGGAAACGCCAGAAAATGAGAGGGATTGTACCTGGTGGGAATGAAATGAACACCGTTGCTGTTCTGGACGAAGCTGTTCAGTACTTGAAGTCTCTCAAGGTTGAGCTGCAGAAGATTGGAGTTGAGAATTTGAACAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

167

Amino Acids

18.51

Weight (kDa)

9.32

Isoelectric Point (pI)

40.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
bHLH_SAC51 PF23173 126 - 165 5.3e-12 Transcription factor SAC51 bHLH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 3 cut(s) 37, 66, 487
AfaI GTAC 3 cut(s) 220, 390, 443
AfiI CCNNNNNNNGG 1 cut(s) 28
AgsI TTSAA 7 cut(s) 37, 43, 114, 283, 317, 448, 493
AjnI CCWGG 1 cut(s) 391
AluBI AGCT 5 cut(s) 166, 190, 348, 434, 466
AluI AGCT 5 cut(s) 166, 190, 348, 434, 466
Alw26I GTCTC 1 cut(s) 456
AlwNI CAGNNNCTG 1 cut(s) 348
ApeKI GCWGC 7 cut(s) 163, 301, 339, 342, 345, 348, 466
ApoI RAATTY 3 cut(s) 37, 66, 487
Asp700I GAANNNNTTC 3 cut(s) 67, 251, 435
AspS9I GGNCC 1 cut(s) 142
AvaII GGWCC 1 cut(s) 142
BbsI GAAGAC 1 cut(s) 315
BbvI GCAGC 7 cut(s) 175, 288, 335, 351, 354, 357, 453
BciT130I CCWGG 1 cut(s) 393
BclI TGATCA 1 cut(s) 90
BcoDI GTCTC 1 cut(s) 456
BfmI CTRYAG 1 cut(s) 467
BisI GCNGC 7 cut(s) 164, 302, 340, 343, 346, 349, 467
BlsI GCNGC 7 cut(s) 165, 303, 341, 344, 347, 350, 468
BmcAI AGTACT 1 cut(s) 443
Bme1390I CCNGG 1 cut(s) 393
Bme18I GGWCC 1 cut(s) 142
BmgT120I GGNCC 1 cut(s) 142
BmrFI CCNGG 1 cut(s) 393
BmsI GCATC 1 cut(s) 62
BpiI GAAGAC 1 cut(s) 315
BpmI CTGGAG 1 cut(s) 253
BpuEI CTTGAG 1 cut(s) 440
BsaJI CCNNGG 1 cut(s) 27
Bsc4I CCNNNNNNNGG 1 cut(s) 28
Bse1I ACTGG 2 cut(s) 144, 270
BseBI CCWGG 1 cut(s) 393
BseDI CCNNGG 1 cut(s) 27
BseGI GGATG 1 cut(s) 170
BseLI CCNNNNNNNGG 1 cut(s) 28
BseNI ACTGG 2 cut(s) 144, 270
BseXI GCAGC 7 cut(s) 175, 288, 335, 351, 354, 357, 453
BsgI GTGCAG 1 cut(s) 182
BslI CCNNNNNNNGG 1 cut(s) 28
BsmAI GTCTC 1 cut(s) 456
Bsp143I GATC 1 cut(s) 90
Bsp19I CCATGG 1 cut(s) 27
BspMAI CTGCAG 1 cut(s) 471
BsrI ACTGG 2 cut(s) 144, 270
BssECI CCNNGG 1 cut(s) 27
BssMI GATC 1 cut(s) 90
BssT1I CCWWGG 1 cut(s) 27
Bst2UI CCWGG 1 cut(s) 393
Bst4CI ACNGT 2 cut(s) 356, 415
Bst6I CTCTTC 1 cut(s) 204
BstAPI GCANNNNNTGC 1 cut(s) 348
BstDSI CCRYGG 1 cut(s) 27
BstF5I GGATG 1 cut(s) 170
BstKTI GATC 1 cut(s) 93
BstMAI GTCTC 1 cut(s) 456
BstMBI GATC 1 cut(s) 90
BstMWI GCNNNNNNNGC 3 cut(s) 336, 345, 348
BstNI CCWGG 1 cut(s) 393
BstSCI CCNGG 1 cut(s) 391
BstSFI CTRYAG 1 cut(s) 467
BstV1I GCAGC 7 cut(s) 175, 288, 335, 351, 354, 357, 453
BstV2I GAAGAC 1 cut(s) 315
BtgI CCRYGG 1 cut(s) 27
BtsCI GGATG 1 cut(s) 170
BtsIMutI CAGTG 1 cut(s) 361
CaiI CAGNNNCTG 1 cut(s) 348
Cfr13I GGNCC 1 cut(s) 142
CsiI ACCWGGT 1 cut(s) 391
Csp6I GTAC 3 cut(s) 219, 389, 442
CviAII CATG 2 cut(s) 28, 233
CviJI RGCY 6 cut(s) 166, 190, 301, 348, 434, 466
CviKI_1 RGCY 6 cut(s) 166, 190, 301, 348, 434, 466
CviQI GTAC 3 cut(s) 219, 389, 442
DpnI GATC 1 cut(s) 92
DpnII GATC 1 cut(s) 90
DraI TTTAAA 1 cut(s) 18
Eam1104I CTCTTC 1 cut(s) 204
EarI CTCTTC 1 cut(s) 204
Eco130I CCWWGG 1 cut(s) 27
Eco47I GGWCC 1 cut(s) 142
EcoRII CCWGG 1 cut(s) 391
EcoT14I CCWWGG 1 cut(s) 27
ErhI CCWWGG 1 cut(s) 27
FaeI CATG 2 cut(s) 31, 236
FaiI YATR 6 cut(s) 29, 109, 201, 234, 243, 276
FatI CATG 2 cut(s) 27, 232
FbaI TGATCA 1 cut(s) 90
Fnu4HI GCNGC 7 cut(s) 164, 302, 340, 343, 346, 349, 467
FokI GGATG 1 cut(s) 157
Fsp4HI GCNGC 7 cut(s) 164, 302, 340, 343, 346, 349, 467
GluI GCNGC 7 cut(s) 164, 302, 340, 343, 346, 349, 467
GsuI CTGGAG 1 cut(s) 253
Hin1II CATG 2 cut(s) 31, 236
HinfI GANTC 1 cut(s) 260
Hpy188I TCNGA 1 cut(s) 259
Hpy188III TCNNGA 2 cut(s) 118, 425
HpyAV CCTTC 1 cut(s) 286
HpyCH4III ACNGT 2 cut(s) 356, 415
HpyCH4V TGCA 3 cut(s) 163, 351, 469
HpyF10VI GCNNNNNNNGC 3 cut(s) 336, 345, 348
Hsp92II CATG 2 cut(s) 31, 236
Ksp22I TGATCA 1 cut(s) 90
Kzo9I GATC 1 cut(s) 90
LmnI GCTCC 2 cut(s) 187, 272
Lsp1109I GCAGC 7 cut(s) 175, 288, 335, 351, 354, 357, 453
LweI GCATC 1 cut(s) 62
MabI ACCWGGT 1 cut(s) 391
MalI GATC 1 cut(s) 92
MboI GATC 1 cut(s) 90
MboII GAAGA 7 cut(s) 69, 75, 206, 221, 300, 315, 484
MluCI AATT 6 cut(s) 37, 66, 122, 134, 238, 487
MnlI CCTC 4 cut(s) 166, 199, 205, 374
MroXI GAANNNNTTC 3 cut(s) 67, 251, 435
MseI TTAA 2 cut(s) 17, 152
MspA1I CMGCKG 1 cut(s) 348
MspR9I CCNGG 1 cut(s) 393
MvaI CCWGG 1 cut(s) 393
MwoI GCNNNNNNNGC 3 cut(s) 336, 345, 348
NcoI CCATGG 1 cut(s) 27
NdeII GATC 1 cut(s) 90
NlaIII CATG 2 cut(s) 31, 236
PdmI GAANNNNTTC 3 cut(s) 67, 251, 435
PfeI GAWTC 1 cut(s) 260
PkrI GCNGC 7 cut(s) 165, 303, 341, 344, 347, 350, 468
Psp6I CCWGG 1 cut(s) 391
PspGI CCWGG 1 cut(s) 391
PspPI GGNCC 1 cut(s) 142
PstI CTGCAG 1 cut(s) 471
PstNI CAGNNNCTG 1 cut(s) 348
PvuII CAGCTG 1 cut(s) 348
RsaI GTAC 3 cut(s) 220, 390, 443
RsaNI GTAC 3 cut(s) 219, 389, 442
SaqAI TTAA 2 cut(s) 17, 152
SatI GCNGC 7 cut(s) 164, 302, 340, 343, 346, 349, 467
Sau3AI GATC 1 cut(s) 90
Sau96I GGNCC 1 cut(s) 142
ScaI AGTACT 1 cut(s) 443
ScrFI CCNGG 1 cut(s) 393
SexAI ACCWGGT 1 cut(s) 391
SfaNI GCATC 1 cut(s) 62
SfcI CTRYAG 1 cut(s) 467
SinI GGWCC 1 cut(s) 142
SmlI CTYRAG 1 cut(s) 455
SmoI CTYRAG 1 cut(s) 455
Sse9I AATT 6 cut(s) 37, 66, 122, 134, 238, 487
StyD4I CCNGG 1 cut(s) 391
StyI CCWWGG 1 cut(s) 27
TaaI ACNGT 2 cut(s) 356, 415
TaqI TCGA 3 cut(s) 61, 80, 96
TasI AATT 6 cut(s) 37, 66, 122, 134, 238, 487
TatI WGTACW 2 cut(s) 218, 441
TfiI GAWTC 1 cut(s) 260
Tru1I TTAA 2 cut(s) 17, 152
Tru9I TTAA 2 cut(s) 17, 152
TscAI CASTG 1 cut(s) 361
TseI GCWGC 7 cut(s) 163, 301, 339, 342, 345, 348, 466
TspDTI ATGAA 3 cut(s) 60, 417, 422
TspRI CASTG 1 cut(s) 361
VpaK11BI GGWCC 1 cut(s) 142
XapI RAATTY 3 cut(s) 37, 66, 487
XcmI CCANNNNNNNNNTGG 1 cut(s) 136
XmnI GAANNNNTTC 3 cut(s) 67, 251, 435
ZrmI AGTACT 1 cut(s) 443
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.