AT1G30300
ERF Family

Beta-lactamase superfamily domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0009230
Physical Location & Seq
Forward (+)
10672973 .. 10675455
2483 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G30300.3

Sequence Viewer

Length: 975 bp
ATGATGGAAAGTTCGATTCCGTCTGAGAACGGCTCCGTTACCTCTGATCGTGATAGATCGGCTCTAATATTCCTAGGAACTGGATGTTCAAGCGCGGTACCTAACGCAATGTGCTTGATCCAGCGATCTGATAATCCCTGCTATGTTTGTTCCCAGTCTCTTTCAATCCCGCCTGAGAAAAATCCTAATTACAGGGGGAATACTTCACTGCTCATTGATTATTGCCAAAGTGATGGCAAACATAAATACATTCAAATCGATGTTGGCAAGACTTTCAGAGAACAAGTCCTTCGTTGGTTTACTCTTCACAAGATTCCTCAAGTTGATTCTATAATTTTGACTCATGAACATGCTGATGCAGTACTTGGCCTGGATGATATACGTTCAGTGCAACCATTTAGTCCTACTAATGATATTGATCCTACTCCAATTTTTGTAAGCCAATACGCTATGGAAAGCCTTGCTGTGAAGTTCCCGTATTTGGTTCAGAAGAAACTTAAAGAAGGCCAAGAAGTTAGGCGAGTAGCACAGCTAGATTGGAGAGTAATTGAAGAAGATTGTGAGAAGCCATTTGTAGCTTCAGGTTTATCATTCACGCCACTTCCAGTGATGCATGGAGAAGACTATGTATGTCTTGGCTTCCTTTTTGGTGAAAAATCAAGAGTGGCTTATATATCCGATGTTTCACGGTTTCCTCCAAACACCGAGTATGCTATATCAAAATCTGGTGGTGGACAATTGGATCTCCTTATTTTGGATACATTATATAAGACAGGATCCCACAACACTCACTTATGTTTCCCGCAGACACTAGACACAATCAAAAGACTGAGTCCAAAAAGGGCTCTTCTGATTGGAATGACTCACGAGTTTGATCATCACAAGGACAACGAGTTTCTTGAAGAATGGTCTAAAAGGGAAGGGATTTCAGTAAAACTCGCTCATGATGGCTTGAGAGTCCCAATAGACCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00440 map00440
Pfam Domains
Protein Families
Lactamase_B_2

Protein Analysis

324

Amino Acids

36.48

Weight (kDa)

5.78

Isoelectric Point (pI)

46.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Anti-Pycsar_Apyc1 PF23023 80 - 234 1.8e-07 Anti-Pycsar protein Apyc1
Lactamase_B_2 PF12706 84 - 286 5.8e-23 Beta-lactamase superfamily domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 97
AccB1I GGYRCC 1 cut(s) 97
AccII CGCG 1 cut(s) 95
AciI CCGC 3 cut(s) 95, 170, 803
AclWI GGATC 5 cut(s) 112, 413, 750, 771, 784
AcuI CTGAAG 1 cut(s) 564
AfaI GTAC 2 cut(s) 99, 363
AfiI CCNNNNNNNGG 2 cut(s) 481, 754
AgsI TTSAA 5 cut(s) 90, 165, 254, 551, 902
AjnI CCWGG 1 cut(s) 369
AluBI AGCT 2 cut(s) 532, 578
AluI AGCT 2 cut(s) 532, 578
Alw26I GTCTC 1 cut(s) 162
AlwI GGATC 5 cut(s) 112, 413, 750, 771, 784
AoxI GGCC 2 cut(s) 367, 505
Asp718I GGTACC 1 cut(s) 97
AspA2I CCTAGG 1 cut(s) 73
AspLEI GCGC 1 cut(s) 95
AsuHPI GGTGA 1 cut(s) 662
AvrII CCTAGG 1 cut(s) 73
BamHI GGATCC 1 cut(s) 776
BanI GGYRCC 1 cut(s) 97
BanII GRGCYC 1 cut(s) 847
BauI CACGAG 1 cut(s) 866
BbsI GAAGAC 1 cut(s) 627
BccI CCATC 2 cut(s) 227, 941
BceAI ACGGC 1 cut(s) 46
BciT130I CCWGG 1 cut(s) 371
BciVI GTATCC 1 cut(s) 751
BclI TGATCA 1 cut(s) 874
BcoDI GTCTC 1 cut(s) 162
BfaI CTAG 3 cut(s) 74, 533, 812
BfuI GTATCC 1 cut(s) 751
BlnI CCTAGG 1 cut(s) 73
BmcAI AGTACT 1 cut(s) 363
Bme1390I CCNGG 1 cut(s) 371
BmiI GGNNCC 3 cut(s) 34, 99, 778
BmrFI CCNGG 1 cut(s) 371
BmrI ACTGGG 1 cut(s) 148
BmsI GCATC 2 cut(s) 346, 600
BmuI ACTGGG 1 cut(s) 148
BpiI GAAGAC 1 cut(s) 627
BplI GAGNNNNNCTC 2 cut(s) 17, 49
BpuEI CTTGAG 2 cut(s) 303, 973
Bsa29I ATCGAT 1 cut(s) 258
BsaBI GATNNNNATC 1 cut(s) 417
BsaJI CCNNGG 1 cut(s) 73
Bsc4I CCNNNNNNNGG 2 cut(s) 481, 754
Bse1I ACTGG 3 cut(s) 85, 154, 605
Bse3DI GCAATG 1 cut(s) 114
Bse8I GATNNNNATC 1 cut(s) 417
BseBI CCWGG 1 cut(s) 371
BseCI ATCGAT 1 cut(s) 258
BseDI CCNNGG 1 cut(s) 73
BseGI GGATG 2 cut(s) 89, 379
BseJI GATNNNNATC 1 cut(s) 417
BseLI CCNNNNNNNGG 2 cut(s) 481, 754
BseMI GCAATG 1 cut(s) 114
BseMII CTCAG 3 cut(s) 15, 165, 821
BseNI ACTGG 3 cut(s) 85, 154, 605
Bsh1236I CGCG 1 cut(s) 95
BshFI GGCC 2 cut(s) 369, 507
BshNI GGYRCC 1 cut(s) 97
BshVI ATCGAT 1 cut(s) 258
BslFI GGGAC 1 cut(s) 944
BslI CCNNNNNNNGG 2 cut(s) 481, 754
BsmAI GTCTC 1 cut(s) 162
BsmFI GGGAC 1 cut(s) 944
BsnI GGCC 2 cut(s) 369, 507
Bsp1286I GDGCHC 1 cut(s) 847
Bsp143I GATC 8 cut(s) 46, 56, 117, 125, 418, 742, 776, 874
BspACI CCGC 3 cut(s) 95, 170, 803
BspANI GGCC 2 cut(s) 369, 507
BspCNI CTCAG 3 cut(s) 16, 166, 822
BspDI ATCGAT 1 cut(s) 258
BspFNI CGCG 1 cut(s) 95
BspHI TCATGA 2 cut(s) 343, 943
BspLI GGNNCC 3 cut(s) 34, 99, 778
BspPI GGATC 5 cut(s) 112, 413, 750, 771, 784
BspQI GCTCTTC 1 cut(s) 852
BspT107I GGYRCC 1 cut(s) 97
BsrDI GCAATG 1 cut(s) 114
BsrI ACTGG 3 cut(s) 85, 154, 605
BssECI CCNNGG 1 cut(s) 73
BssMI GATC 8 cut(s) 46, 56, 117, 125, 418, 742, 776, 874
BssSI CACGAG 1 cut(s) 866
BssT1I CCWWGG 1 cut(s) 73
Bst2BI CACGAG 1 cut(s) 866
Bst2UI CCWGG 1 cut(s) 371
Bst4CI ACNGT 1 cut(s) 690
Bst6I CTCTTC 2 cut(s) 309, 852
BstDEI CTNAG 3 cut(s) 24, 174, 830
BstF5I GGATG 2 cut(s) 89, 379
BstFNI CGCG 1 cut(s) 95
BstHHI GCGC 1 cut(s) 95
BstKTI GATC 8 cut(s) 49, 59, 120, 128, 421, 745, 779, 877
BstMAI GTCTC 1 cut(s) 162
BstMBI GATC 8 cut(s) 46, 56, 117, 125, 418, 742, 776, 874
BstNI CCWGG 1 cut(s) 371
BstNSI RCATGY 1 cut(s) 353
BstSCI CCNGG 1 cut(s) 369
BstUI CGCG 1 cut(s) 95
BstV2I GAAGAC 1 cut(s) 627
BstX2I RGATCY 2 cut(s) 742, 776
BstXI CCANNNNNNTGG 1 cut(s) 233
BstYI RGATCY 2 cut(s) 742, 776
Bsu15I ATCGAT 1 cut(s) 258
BsuI GTATCC 1 cut(s) 751
BsuRI GGCC 2 cut(s) 369, 507
BsuTUI ATCGAT 1 cut(s) 258
BtsCI GGATG 2 cut(s) 89, 379
BtsI GCAGTG 1 cut(s) 206
BtsIMutI CAGTG 3 cut(s) 206, 393, 612
CciI TCATGA 2 cut(s) 343, 943
CfoI GCGC 1 cut(s) 95
ClaI ATCGAT 1 cut(s) 258
Csp6I GTAC 2 cut(s) 98, 362
CviAII CATG 4 cut(s) 344, 350, 614, 944
CviQI GTAC 2 cut(s) 98, 362
DdeI CTNAG 3 cut(s) 24, 174, 830
DpnI GATC 8 cut(s) 48, 58, 119, 127, 420, 744, 778, 876
DpnII GATC 8 cut(s) 46, 56, 117, 125, 418, 742, 776, 874
Eam1104I CTCTTC 2 cut(s) 309, 852
EarI CTCTTC 2 cut(s) 309, 852
Eco130I CCWWGG 1 cut(s) 73
Eco24I GRGCYC 1 cut(s) 847
Eco57I CTGAAG 1 cut(s) 564
EcoRII CCWGG 1 cut(s) 369
EcoT14I CCWWGG 1 cut(s) 73
EcoT22I ATGCAT 1 cut(s) 615
EcoT38I GRGCYC 1 cut(s) 847
ErhI CCWWGG 1 cut(s) 73
FaeI CATG 4 cut(s) 347, 353, 617, 947
FalI AAGNNNNNCTT 2 cut(s) 652, 684
FaqI GGGAC 1 cut(s) 944
FatI CATG 4 cut(s) 343, 349, 613, 943
FauI CCCGC 2 cut(s) 177, 810
FbaI TGATCA 1 cut(s) 874
FokI GGATG 2 cut(s) 96, 386
FriOI GRGCYC 1 cut(s) 847
FspBI CTAG 3 cut(s) 74, 533, 812
GlaI GCGC 1 cut(s) 94
HaeIII GGCC 2 cut(s) 369, 507
HhaI GCGC 1 cut(s) 95
Hin1II CATG 4 cut(s) 347, 353, 617, 947
Hin6I GCGC 1 cut(s) 93
HinP1I GCGC 1 cut(s) 93
HinfI GANTC 7 cut(s) 16, 313, 326, 340, 832, 862, 957
HphI GGTGA 1 cut(s) 662
Hpy166II GTNNAC 2 cut(s) 300, 734
Hpy188I TCNGA 7 cut(s) 25, 46, 130, 278, 489, 679, 852
Hpy188III TCNNGA 6 cut(s) 50, 344, 660, 866, 899, 944
Hpy8I GTNNAC 2 cut(s) 300, 734
HpyAV CCTTC 3 cut(s) 299, 497, 914
HpyCH4III ACNGT 1 cut(s) 690
HpyCH4IV ACGT 1 cut(s) 382
HpyCH4V TGCA 3 cut(s) 359, 391, 613
HpyF3I CTNAG 3 cut(s) 24, 174, 830
HpySE526I ACGT 1 cut(s) 382
Hsp92II CATG 4 cut(s) 347, 353, 617, 947
HspAI GCGC 1 cut(s) 93
KpnI GGTACC 1 cut(s) 101
Ksp22I TGATCA 1 cut(s) 874
Kzo9I GATC 8 cut(s) 46, 56, 117, 125, 418, 742, 776, 874
LguI GCTCTTC 1 cut(s) 852
LmnI GCTCC 1 cut(s) 38
LweI GCATC 2 cut(s) 346, 600
MaeI CTAG 3 cut(s) 74, 533, 812
MaeII ACGT 1 cut(s) 382
MaeIII GTNAC 1 cut(s) 37
MalI GATC 8 cut(s) 48, 58, 119, 127, 420, 744, 778, 876
MboI GATC 8 cut(s) 46, 56, 117, 125, 418, 742, 776, 874
MboII GAAGA 7 cut(s) 296, 502, 563, 566, 632, 839, 914
MfeI CAATTG 1 cut(s) 737
MflI RGATCY 2 cut(s) 742, 776
MhlI GDGCHC 1 cut(s) 847
MluCI AATT 5 cut(s) 187, 333, 429, 546, 737
MlyI GAGTC 4 cut(s) 334, 841, 856, 966
MnlI CCTC 3 cut(s) 52, 327, 705
Mph1103I ATGCAT 1 cut(s) 615
MseI TTAA 1 cut(s) 498
MslI CAYNNNNRTG 2 cut(s) 348, 354
MspR9I CCNGG 1 cut(s) 371
MunI CAATTG 1 cut(s) 737
MvaI CCWGG 1 cut(s) 371
MvnI CGCG 1 cut(s) 95
NdeII GATC 8 cut(s) 46, 56, 117, 125, 418, 742, 776, 874
NlaIII CATG 4 cut(s) 347, 353, 617, 947
NlaIV GGNNCC 3 cut(s) 34, 99, 778
NsiI ATGCAT 1 cut(s) 615
NspI RCATGY 1 cut(s) 353
PagI TCATGA 2 cut(s) 343, 943
PciSI GCTCTTC 1 cut(s) 852
PfeI GAWTC 3 cut(s) 16, 313, 326
PflFI GACNNNGTC 1 cut(s) 831
PleI GAGTC 4 cut(s) 334, 840, 856, 965
PpsI GAGTC 4 cut(s) 334, 840, 856, 965
Psp6I CCWGG 1 cut(s) 369
PspGI CCWGG 1 cut(s) 369
PspN4I GGNNCC 3 cut(s) 34, 99, 778
PsuI RGATCY 2 cut(s) 742, 776
PsyI GACNNNGTC 1 cut(s) 831
RsaI GTAC 2 cut(s) 99, 363
RsaNI GTAC 2 cut(s) 98, 362
RseI CAYNNNNRTG 2 cut(s) 348, 354
SapI GCTCTTC 1 cut(s) 852
SaqAI TTAA 1 cut(s) 498
Sau3AI GATC 8 cut(s) 46, 56, 117, 125, 418, 742, 776, 874
ScaI AGTACT 1 cut(s) 363
SchI GAGTC 4 cut(s) 334, 841, 856, 966
ScrFI CCNGG 1 cut(s) 371
SduI GDGCHC 1 cut(s) 847
SetI ASST 7 cut(s) 44, 103, 385, 534, 580, 586, 972
SfaNI GCATC 2 cut(s) 346, 600
SmiMI CAYNNNNRTG 2 cut(s) 348, 354
SmlI CTYRAG 2 cut(s) 318, 952
SmoI CTYRAG 2 cut(s) 318, 952
Sse9I AATT 5 cut(s) 187, 333, 429, 546, 737
SsiI CCGC 3 cut(s) 95, 170, 803
SspI AATATT 1 cut(s) 69
SspMI CTAG 3 cut(s) 74, 533, 812
StyD4I CCNGG 1 cut(s) 369
StyI CCWWGG 1 cut(s) 73
TaaI ACNGT 1 cut(s) 690
TaiI ACGT 1 cut(s) 385
TaqI TCGA 2 cut(s) 14, 258
TasI AATT 5 cut(s) 187, 333, 429, 546, 737
TatI WGTACW 1 cut(s) 361
TfiI GAWTC 3 cut(s) 16, 313, 326
Tru1I TTAA 1 cut(s) 498
Tru9I TTAA 1 cut(s) 498
TscAI CASTG 3 cut(s) 213, 393, 612
TspDTI ATGAA 1 cut(s) 360
TspGWI ACGGA 2 cut(s) 9, 25
TspRI CASTG 3 cut(s) 213, 393, 612
Tth111I GACNNNGTC 1 cut(s) 831
XceI RCATGY 1 cut(s) 353
XmaJI CCTAGG 1 cut(s) 73
XspI CTAG 3 cut(s) 74, 533, 812
ZrmI AGTACT 1 cut(s) 363
Zsp2I ATGCAT 1 cut(s) 615
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.