Rh5DG113600

Hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0009230
Physical Location & Seq
Forward (+)
10279140 .. 10283903
4764 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG113600.1

Sequence Viewer

Length: 714 bp
ATGGCGGCCCACGACGGAATCTGTACCGCCGGCGACGCCGCAGCCCGATCGGCTCTGATCTTCGTCGGCACCGGCTGCTCCAGCTCCGTCCCCAACTTAAGGTGCCTTATCCAGCCCTCCGATCCCCCCTGCCACGTCTGCTCTCAGGCCCTCTCCCTTCCCCCCGACCAAAACCCTAACTACAGGTGCAATACGTCGCTTCTGATCGATTATTGCCGGAGCGACGGCGAACACAGGTACGTGTTGATCGATGTGGGGAAGACGTTCAGGGAGCAAGTGCTGCGGTGGTTCACTCGCTATAAGATTCCTAGAGTTGATTCTATTATTTTGACTCATGAACATGCTGATGCAGTTCTTGGTCTGGATGACATACGTGCTGTACAGCCACATAGTTCCACAAATAACATTGAGCCTACTCCCATCTACCTAACTCAATTTGCAATGGAAAGCATTGCAGTGAAGTTCCCTTACTTGGTTCAGAAAAAACTTAAGGAAGGCCAAGAAGTGAGACGAGTTGCACAACTCGACTGGAAAATTATTGAGGACCATTGTGAAAAACCTTTTGTTGCATCAGGCCTACAATTTGTTCCTTTGCCAGTGATGCATGGGGAAGATTACGTCTGCTTGGGGTATCTATTTGGTGAACAGTGTCGAGTAGCTTATATATCTGATGTGTCACGTTTTCTTTCAAGCACAGAGCATTGTGAGTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00440 map00440
Pfam Domains
Protein Families
Lactamase_B_2

Protein Analysis

237

Amino Acids

26.57

Weight (kDa)

5.83

Isoelectric Point (pI)

46.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Anti-Pycsar_Apyc1 PF23023 77 - 225 5.6e-06 Anti-Pycsar protein Apyc1
Lactamase_B_2 PF12706 79 - 228 5.9e-16 Beta-lactamase superfamily domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 68, 102
AciI CCGC 4 cut(s) 5, 27, 39, 283
AclWI GGATC 1 cut(s) 116
AcyI GRCGYC 1 cut(s) 36
AfaI GTAC 3 cut(s) 25, 239, 381
AfiI CCNNNNNNNGG 2 cut(s) 99, 472
AflII CTTAAG 2 cut(s) 97, 488
AflIII ACRYGT 1 cut(s) 240
AgsI TTSAA 1 cut(s) 690
AjiI CACGTC 1 cut(s) 136
AluBI AGCT 2 cut(s) 84, 659
AluI AGCT 2 cut(s) 84, 659
Alw26I GTCTC 1 cut(s) 502
AlwI GGATC 1 cut(s) 116
AoxI GGCC 4 cut(s) 6, 147, 496, 574
ApeKI GCWGC 3 cut(s) 41, 75, 280
Asp700I GAANNNNTTC 1 cut(s) 263
AspS9I GGNCC 3 cut(s) 7, 148, 544
AsuHPI GGTGA 1 cut(s) 653
AvaII GGWCC 1 cut(s) 544
BanI GGYRCC 2 cut(s) 68, 102
BarI GAAGNNNNNNTAC 2 cut(s) 452, 484
BbsI GAAGAC 1 cut(s) 266
BbvI GCAGC 3 cut(s) 53, 62, 267
BccI CCATC 1 cut(s) 428
BceAI ACGGC 1 cut(s) 241
BcgI CGANNNNNNTGC 2 cut(s) 30, 64
BcoDI GTCTC 1 cut(s) 502
BfaI CTAG 1 cut(s) 309
BfmI CTRYAG 1 cut(s) 181
BfrI CTTAAG 2 cut(s) 97, 488
BglI GCCNNNNNGGC 1 cut(s) 50
BisI GCNGC 5 cut(s) 6, 39, 42, 76, 281
BlsI GCNGC 5 cut(s) 7, 40, 43, 77, 282
Bme18I GGWCC 1 cut(s) 544
BmgBI CACGTC 1 cut(s) 136
BmgT120I GGNCC 3 cut(s) 7, 148, 544
BmiI GGNNCC 2 cut(s) 70, 104
BmsI GCATC 3 cut(s) 337, 578, 591
BpiI GAAGAC 1 cut(s) 266
BpmI CTGGAG 1 cut(s) 64
Bsa29I ATCGAT 2 cut(s) 207, 249
BsaAI YACGTR 2 cut(s) 241, 374
BsaHI GRCGYC 1 cut(s) 36
Bsc4I CCNNNNNNNGG 2 cut(s) 99, 472
Bse118I RCCGGY 2 cut(s) 29, 71
Bse1I ACTGG 2 cut(s) 533, 596
Bse3DI GCAATG 2 cut(s) 447, 450
BseCI ATCGAT 2 cut(s) 207, 249
BseGI GGATG 1 cut(s) 370
BseLI CCNNNNNNNGG 2 cut(s) 99, 472
BseMI GCAATG 2 cut(s) 447, 450
BseMII CTCAG 1 cut(s) 158
BseNI ACTGG 2 cut(s) 533, 596
BseXI GCAGC 3 cut(s) 53, 62, 267
Bsh1285I CGRYCG 1 cut(s) 50
BshFI GGCC 4 cut(s) 8, 149, 498, 576
BshNI GGYRCC 2 cut(s) 68, 102
BshVI ATCGAT 2 cut(s) 207, 249
BsiEI CGRYCG 1 cut(s) 50
BsiSI CCGG 3 cut(s) 30, 72, 217
BslFI GGGAC 1 cut(s) 74
BslI CCNNNNNNNGG 2 cut(s) 99, 472
BsmAI GTCTC 1 cut(s) 502
BsmBI CGTCTC 1 cut(s) 502
BsmFI GGGAC 1 cut(s) 74
BsnI GGCC 4 cut(s) 8, 149, 498, 576
Bsp1407I TGTACA 1 cut(s) 379
Bsp143I GATC 5 cut(s) 47, 57, 121, 204, 246
BspACI CCGC 4 cut(s) 5, 27, 39, 283
BspANI GGCC 4 cut(s) 8, 149, 498, 576
BspCNI CTCAG 1 cut(s) 157
BspDI ATCGAT 2 cut(s) 207, 249
BspHI TCATGA 1 cut(s) 334
BspLI GGNNCC 2 cut(s) 70, 104
BspPI GGATC 1 cut(s) 116
BspT107I GGYRCC 2 cut(s) 68, 102
BspTI CTTAAG 2 cut(s) 97, 488
BsrDI GCAATG 2 cut(s) 447, 450
BsrFI RCCGGY 2 cut(s) 29, 71
BsrGI TGTACA 1 cut(s) 379
BsrI ACTGG 2 cut(s) 533, 596
BssAI RCCGGY 2 cut(s) 29, 71
BssMI GATC 5 cut(s) 47, 57, 121, 204, 246
BssNI GRCGYC 1 cut(s) 36
Bst4CI ACNGT 1 cut(s) 648
BstACI GRCGYC 1 cut(s) 36
BstAFI CTTAAG 2 cut(s) 97, 488
BstAPI GCANNNNNTGC 2 cut(s) 75, 280
BstAUI TGTACA 1 cut(s) 379
BstBAI YACGTR 2 cut(s) 241, 374
BstC8I GCNNGC 1 cut(s) 31
BstDEI CTNAG 1 cut(s) 144
BstF5I GGATG 1 cut(s) 370
BstKTI GATC 5 cut(s) 50, 60, 124, 207, 249
BstMAI GTCTC 1 cut(s) 502
BstMBI GATC 5 cut(s) 47, 57, 121, 204, 246
BstMCI CGRYCG 1 cut(s) 50
BstMWI GCNNNNNNNGC 7 cut(s) 35, 50, 75, 81, 138, 280, 601
BstNSI RCATGY 1 cut(s) 344
BstSFI CTRYAG 1 cut(s) 181
BstV1I GCAGC 3 cut(s) 53, 62, 267
BstV2I GAAGAC 1 cut(s) 266
Bsu15I ATCGAT 2 cut(s) 207, 249
BsuRI GGCC 4 cut(s) 8, 149, 498, 576
BsuTUI ATCGAT 2 cut(s) 207, 249
BtrI CACGTC 1 cut(s) 136
BtsCI GGATG 1 cut(s) 370
BtsI GCAGTG 1 cut(s) 462
BtsIMutI CAGTG 3 cut(s) 462, 603, 653
Cac8I GCNNGC 1 cut(s) 31
CciI TCATGA 1 cut(s) 334
Cfr10I RCCGGY 2 cut(s) 29, 71
Cfr13I GGNCC 3 cut(s) 7, 148, 544
ClaI ATCGAT 2 cut(s) 207, 249
CseI GACGC 1 cut(s) 44
Csp6I GTAC 3 cut(s) 24, 238, 380
CviAII CATG 3 cut(s) 335, 341, 605
CviQI GTAC 3 cut(s) 24, 238, 380
DdeI CTNAG 1 cut(s) 144
DpnI GATC 5 cut(s) 49, 59, 123, 206, 248
DpnII GATC 5 cut(s) 47, 57, 121, 204, 246
Eco147I AGGCCT 1 cut(s) 576
Eco47I GGWCC 1 cut(s) 544
EcoO109I RGGNCCY 1 cut(s) 148
EcoT22I ATGCAT 1 cut(s) 606
Esp3I CGTCTC 1 cut(s) 502
FaeI CATG 3 cut(s) 338, 344, 608
FaiI YATR 8 cut(s) 300, 336, 342, 371, 390, 606, 663, 665
FaqI GGGAC 1 cut(s) 74
FatI CATG 3 cut(s) 334, 340, 604
Fnu4HI GCNGC 5 cut(s) 6, 39, 42, 76, 281
FokI GGATG 1 cut(s) 377
Fsp4HI GCNGC 5 cut(s) 6, 39, 42, 76, 281
FspBI CTAG 1 cut(s) 309
GluI GCNGC 5 cut(s) 6, 39, 42, 76, 281
GsuI CTGGAG 1 cut(s) 64
HaeIII GGCC 4 cut(s) 8, 149, 498, 576
HapII CCGG 3 cut(s) 30, 72, 217
HgaI GACGC 1 cut(s) 44
Hin1I GRCGYC 1 cut(s) 36
Hin1II CATG 3 cut(s) 338, 344, 608
HinfI GANTC 4 cut(s) 18, 304, 317, 331
HpaII CCGG 3 cut(s) 30, 72, 217
HphI GGTGA 1 cut(s) 653
Hpy166II GTNNAC 2 cut(s) 291, 644
Hpy188I TCNGA 5 cut(s) 57, 121, 204, 480, 670
Hpy188III TCNNGA 2 cut(s) 335, 362
Hpy8I GTNNAC 2 cut(s) 291, 644
Hpy99I CGWCG 5 cut(s) 17, 38, 68, 199, 227
HpyAV CCTTC 2 cut(s) 167, 488
HpyCH4III ACNGT 1 cut(s) 648
HpyCH4IV ACGT 7 cut(s) 135, 194, 240, 263, 373, 618, 679
HpyCH4V TGCA 7 cut(s) 189, 350, 440, 455, 518, 569, 604
HpyF10VI GCNNNNNNNGC 7 cut(s) 35, 50, 75, 81, 138, 280, 601
HpyF3I CTNAG 1 cut(s) 144
HpySE526I ACGT 7 cut(s) 135, 194, 240, 263, 373, 618, 679
Hsp92I GRCGYC 1 cut(s) 36
Hsp92II CATG 3 cut(s) 338, 344, 608
KroI GCCGGC 1 cut(s) 29
KroNI GCCGGC 1 cut(s) 31
Kzo9I GATC 5 cut(s) 47, 57, 121, 204, 246
LmnI GCTCC 4 cut(s) 83, 89, 219, 271
Lsp1109I GCAGC 3 cut(s) 53, 62, 267
LweI GCATC 3 cut(s) 337, 578, 591
MaeI CTAG 1 cut(s) 309
MaeII ACGT 7 cut(s) 135, 194, 240, 263, 373, 618, 679
MaeIII GTNAC 1 cut(s) 675
MalI GATC 5 cut(s) 49, 59, 123, 206, 248
MboI GATC 5 cut(s) 47, 57, 121, 204, 246
MboII GAAGA 3 cut(s) 52, 271, 623
MluCI AATT 3 cut(s) 434, 534, 581
MlyI GAGTC 1 cut(s) 325
MnlI CCTC 3 cut(s) 127, 161, 535
Mph1103I ATGCAT 1 cut(s) 606
MreI CGCCGGCG 1 cut(s) 29
MroNI GCCGGC 1 cut(s) 29
MroXI GAANNNNTTC 1 cut(s) 263
MseI TTAA 2 cut(s) 98, 489
MslI CAYNNNNRTG 3 cut(s) 339, 345, 455
MspCI CTTAAG 2 cut(s) 97, 488
MspI CCGG 3 cut(s) 30, 72, 217
MwoI GCNNNNNNNGC 7 cut(s) 35, 50, 75, 81, 138, 280, 601
NaeI GCCGGC 1 cut(s) 31
NdeII GATC 5 cut(s) 47, 57, 121, 204, 246
NgoMIV GCCGGC 1 cut(s) 29
NlaIII CATG 3 cut(s) 338, 344, 608
NlaIV GGNNCC 2 cut(s) 70, 104
NmuCI GTSAC 1 cut(s) 675
NsiI ATGCAT 1 cut(s) 606
NspI RCATGY 1 cut(s) 344
PagI TCATGA 1 cut(s) 334
PceI AGGCCT 1 cut(s) 576
PcsI WCGNNNNNNNCGW 1 cut(s) 246
PdiI GCCGGC 1 cut(s) 31
PdmI GAANNNNTTC 1 cut(s) 263
PfeI GAWTC 3 cut(s) 18, 304, 317
PkrI GCNGC 5 cut(s) 7, 40, 43, 77, 282
Ple19I CGATCG 1 cut(s) 50
PleI GAGTC 1 cut(s) 325
PpsI GAGTC 1 cut(s) 325
Ppu21I YACGTR 2 cut(s) 241, 374
PspN4I GGNNCC 2 cut(s) 70, 104
PspPI GGNCC 3 cut(s) 7, 148, 544
PvuI CGATCG 1 cut(s) 50
RsaI GTAC 3 cut(s) 25, 239, 381
RsaNI GTAC 3 cut(s) 24, 238, 380
RseI CAYNNNNRTG 3 cut(s) 339, 345, 455
SaqAI TTAA 2 cut(s) 98, 489
SatI GCNGC 5 cut(s) 6, 39, 42, 76, 281
Sau3AI GATC 5 cut(s) 47, 57, 121, 204, 246
Sau96I GGNCC 3 cut(s) 7, 148, 544
SchI GAGTC 1 cut(s) 325
SfaNI GCATC 3 cut(s) 337, 578, 591
SfcI CTRYAG 1 cut(s) 181
SgrAI CRCCGGYG 1 cut(s) 29
SinI GGWCC 1 cut(s) 544
SmiMI CAYNNNNRTG 3 cut(s) 339, 345, 455
SmlI CTYRAG 2 cut(s) 97, 488
SmoI CTYRAG 2 cut(s) 97, 488
Sse9I AATT 3 cut(s) 434, 534, 581
SseBI AGGCCT 1 cut(s) 576
SsiI CCGC 4 cut(s) 5, 27, 39, 283
SspMI CTAG 1 cut(s) 309
StuI AGGCCT 1 cut(s) 576
TaaI ACNGT 1 cut(s) 648
TaiI ACGT 7 cut(s) 138, 197, 243, 266, 376, 621, 682
TaqI TCGA 4 cut(s) 207, 249, 525, 652
TasI AATT 3 cut(s) 434, 534, 581
TatI WGTACW 1 cut(s) 379
TauI GCSGC 2 cut(s) 8, 41
TfiI GAWTC 3 cut(s) 18, 304, 317
Tru1I TTAA 2 cut(s) 98, 489
Tru9I TTAA 2 cut(s) 98, 489
TscAI CASTG 3 cut(s) 462, 603, 653
TseFI GTSAC 1 cut(s) 675
TseI GCWGC 3 cut(s) 41, 75, 280
Tsp45I GTSAC 1 cut(s) 675
TspDTI ATGAA 1 cut(s) 351
TspGWI ACGGA 2 cut(s) 30, 76
TspRI CASTG 3 cut(s) 462, 603, 653
Vha464I CTTAAG 2 cut(s) 97, 488
VpaK11BI GGWCC 1 cut(s) 544
XceI RCATGY 1 cut(s) 344
XmnI GAANNNNTTC 1 cut(s) 263
XspI CTAG 1 cut(s) 309
Zsp2I ATGCAT 1 cut(s) 606
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.