RLG00000032151

Hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0009230
Physical Location & Seq
Forward (+)
11434603 .. 11438556
3954 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032151

Sequence Viewer

Length: 969 bp
ATGGCGGCCCAGGACGGAATCTGTACCGCCGGCGACGCCGCAGCAGCCCGATCGGCTGTGATCTTCGTCGGCACCGGCTGCTCCGGCTCCGTCCCCAACTTAATGTGCCTCATCCAGCCCTCCGATCCCCCCTGCCACGTCTGCTCTCGGGCCCTCTCCCTTCCCCCCGACCAAAACCCTAACTACAGGTGCAATACGTCGCTTCTGATCGATTATTGCCGGAGCGACGGCGAACACAGGTACGTGTTGATCGATGTGGGGAAGACGTTCAGGGAGCAAGTGCTGCGGTGGTTCACTCGCTATAAGATTCCTAGAGTTGATTCTATTATTTTGACTCATGAACATGCTGATGCAGTTCTTGGTCTGGATGACATACGTGCTGTACAGCCATATAGTTCCACAAATGACATTGAGCCTACTCCCATCTACCTAACTCAATTTGCAATGGAAAGCATTGCAGTGAAGTTCCCTTACTTGGTTCAGAAAAAAAGTAAGGAAGGCCAAGAAGTGAGACGAGTTGCACAACTCGACTGGAAAATTATTGAGGACCATTGTGAAAAACCTTTTGTTGCATCAGGCCTACAATTTGTTCCTTTGCCAGTGATGCATGGGGAAGATTATGTCTGCTTGGGGTATCTATTTGGTGAACAGTGTCGAGTAGCTTATATATCTGACGTGTCACGTTTTCTTTCAAGCACTGAGCATTTGATTTCAAAAGATGGAGGTGGGCAGTTGGATCTTCTTATCTTGGACACATTATACAAGAATGGATCGCACAATGTTCACTTTTGCTTGCCACAGTCTCTAGAAGCTGTAAAGAGGATTTGTCCTAAACGGGCCCTATTAATTGGAATGACTCATGAGTTTGACCACCATAAGGACAATGAATCTCTTATGGAATGGTCTAAGCGGGAAGGGATACCTGTGCAGCTTGCGCATGATGGTTTGAGAGTCCCCTTAGACCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00440 map00440
Pfam Domains
Protein Families
Lactamase_B_2

Protein Analysis

323

Amino Acids

36.07

Weight (kDa)

5.89

Isoelectric Point (pI)

43.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Anti-Pycsar_Apyc1 PF23023 78 - 267 2.5e-07 Anti-Pycsar protein Apyc1
Lactamase_B_2 PF12706 80 - 284 5.7e-22 Beta-lactamase superfamily domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 936
AccB1I GGYRCC 1 cut(s) 71
AciI CCGC 5 cut(s) 5, 27, 39, 286, 910
AclWI GGATC 3 cut(s) 119, 744, 778
AcyI GRCGYC 1 cut(s) 36
AfaI GTAC 3 cut(s) 25, 242, 384
AfiI CCNNNNNNNGG 2 cut(s) 475, 877
AflIII ACRYGT 2 cut(s) 243, 675
AgsI TTSAA 2 cut(s) 693, 714
AjiI CACGTC 2 cut(s) 139, 676
AjnI CCWGG 1 cut(s) 9
AluBI AGCT 3 cut(s) 662, 812, 931
AluI AGCT 3 cut(s) 662, 812, 931
Alw26I GTCTC 2 cut(s) 505, 807
AlwI GGATC 3 cut(s) 119, 744, 778
Ama87I CYCGRG 1 cut(s) 147
AoxI GGCC 5 cut(s) 6, 150, 499, 577, 837
ApaI GGGCCC 2 cut(s) 154, 841
ApeKI GCWGC 5 cut(s) 41, 44, 78, 283, 928
AseI ATTAAT 1 cut(s) 845
Asp700I GAANNNNTTC 1 cut(s) 266
AspLEI GCGC 1 cut(s) 937
AspS9I GGNCC 6 cut(s) 7, 150, 151, 547, 837, 838
AsuHPI GGTGA 1 cut(s) 656
AvaI CYCGRG 1 cut(s) 147
AvaII GGWCC 1 cut(s) 547
BaeGI GKGCMC 2 cut(s) 154, 841
BanI GGYRCC 1 cut(s) 71
BanII GRGCYC 2 cut(s) 154, 841
BarI GAAGNNNNNNTAC 2 cut(s) 455, 487
BbsI GAAGAC 1 cut(s) 269
BbvI GCAGC 5 cut(s) 53, 56, 65, 270, 940
BccI CCATC 3 cut(s) 431, 713, 935
BceAI ACGGC 1 cut(s) 244
BcgI CGANNNNNNTGC 2 cut(s) 33, 67
BciT130I CCWGG 1 cut(s) 11
BciVI GTATCC 1 cut(s) 912
BcoDI GTCTC 2 cut(s) 505, 807
BfaI CTAG 2 cut(s) 312, 806
BfmI CTRYAG 1 cut(s) 184
BfuI GTATCC 1 cut(s) 912
BglI GCCNNNNNGGC 1 cut(s) 53
BisI GCNGC 7 cut(s) 6, 39, 42, 45, 79, 284, 929
BlsI GCNGC 7 cut(s) 7, 40, 43, 46, 80, 285, 930
Bme1390I CCNGG 1 cut(s) 11
Bme18I GGWCC 1 cut(s) 547
BmeT110I CYCGRG 1 cut(s) 147
BmgBI CACGTC 2 cut(s) 139, 676
BmgT120I GGNCC 6 cut(s) 7, 150, 151, 547, 837, 838
BmiI GGNNCC 4 cut(s) 73, 88, 152, 839
BmrFI CCNGG 1 cut(s) 11
BmsI GCATC 3 cut(s) 340, 581, 594
BpiI GAAGAC 1 cut(s) 269
Bsa29I ATCGAT 2 cut(s) 210, 252
BsaAI YACGTR 2 cut(s) 244, 377
BsaHI GRCGYC 1 cut(s) 36
BsaJI CCNNGG 1 cut(s) 9
Bsc4I CCNNNNNNNGG 2 cut(s) 475, 877
Bse118I RCCGGY 2 cut(s) 29, 74
Bse1I ACTGG 2 cut(s) 536, 599
Bse3DI GCAATG 2 cut(s) 450, 453
BseBI CCWGG 1 cut(s) 11
BseCI ATCGAT 2 cut(s) 210, 252
BseDI CCNNGG 1 cut(s) 9
BseGI GGATG 2 cut(s) 111, 373
BseLI CCNNNNNNNGG 2 cut(s) 475, 877
BseMI GCAATG 2 cut(s) 450, 453
BseMII CTCAG 1 cut(s) 690
BseNI ACTGG 2 cut(s) 536, 599
BseSI GKGCMC 2 cut(s) 154, 841
BseXI GCAGC 5 cut(s) 53, 56, 65, 270, 940
BsgI GTGCAG 1 cut(s) 947
Bsh1285I CGRYCG 1 cut(s) 53
BshFI GGCC 5 cut(s) 8, 152, 501, 579, 839
BshNI GGYRCC 1 cut(s) 71
BshVI ATCGAT 2 cut(s) 210, 252
BsiEI CGRYCG 1 cut(s) 53
BsiHKCI CYCGRG 1 cut(s) 147
BsiSI CCGG 4 cut(s) 30, 75, 84, 220
BslFI GGGAC 2 cut(s) 77, 938
BslI CCNNNNNNNGG 2 cut(s) 475, 877
BsmAI GTCTC 2 cut(s) 505, 807
BsmBI CGTCTC 1 cut(s) 505
BsmFI GGGAC 2 cut(s) 77, 938
BsnI GGCC 5 cut(s) 8, 152, 501, 579, 839
BsoBI CYCGRG 1 cut(s) 147
Bsp120I GGGCCC 2 cut(s) 150, 837
Bsp1286I GDGCHC 2 cut(s) 154, 841
Bsp1407I TGTACA 1 cut(s) 382
Bsp143I GATC 7 cut(s) 50, 60, 124, 207, 249, 736, 770
BspACI CCGC 5 cut(s) 5, 27, 39, 286, 910
BspANI GGCC 5 cut(s) 8, 152, 501, 579, 839
BspCNI CTCAG 1 cut(s) 691
BspDI ATCGAT 2 cut(s) 210, 252
BspHI TCATGA 2 cut(s) 337, 859
BspLI GGNNCC 4 cut(s) 73, 88, 152, 839
BspPI GGATC 3 cut(s) 119, 744, 778
BspT107I GGYRCC 1 cut(s) 71
BsrDI GCAATG 2 cut(s) 450, 453
BsrFI RCCGGY 2 cut(s) 29, 74
BsrGI TGTACA 1 cut(s) 382
BsrI ACTGG 2 cut(s) 536, 599
BssAI RCCGGY 2 cut(s) 29, 74
BssECI CCNNGG 1 cut(s) 9
BssMI GATC 7 cut(s) 50, 60, 124, 207, 249, 736, 770
BssNI GRCGYC 1 cut(s) 36
Bst2UI CCWGG 1 cut(s) 11
Bst4CI ACNGT 2 cut(s) 651, 801
BstACI GRCGYC 1 cut(s) 36
BstAPI GCANNNNNTGC 2 cut(s) 78, 283
BstAUI TGTACA 1 cut(s) 382
BstBAI YACGTR 2 cut(s) 244, 377
BstC8I GCNNGC 3 cut(s) 31, 794, 933
BstDEI CTNAG 3 cut(s) 699, 906, 958
BstF5I GGATG 2 cut(s) 111, 373
BstHHI GCGC 1 cut(s) 937
BstKTI GATC 7 cut(s) 53, 63, 127, 210, 252, 739, 773
BstMAI GTCTC 2 cut(s) 505, 807
BstMBI GATC 7 cut(s) 50, 60, 124, 207, 249, 736, 770
BstMCI CGRYCG 1 cut(s) 53
BstMWI GCNNNNNNNGC 9 cut(s) 35, 44, 53, 78, 84, 141, 283, 604, 934
BstNI CCWGG 1 cut(s) 11
BstNSI RCATGY 1 cut(s) 347
BstSCI CCNGG 1 cut(s) 9
BstSFI CTRYAG 1 cut(s) 184
BstSLI GKGCMC 2 cut(s) 154, 841
BstV1I GCAGC 5 cut(s) 53, 56, 65, 270, 940
BstV2I GAAGAC 1 cut(s) 269
BstX2I RGATCY 1 cut(s) 736
BstYI RGATCY 1 cut(s) 736
Bsu15I ATCGAT 2 cut(s) 210, 252
BsuI GTATCC 1 cut(s) 912
BsuRI GGCC 5 cut(s) 8, 152, 501, 579, 839
BsuTUI ATCGAT 2 cut(s) 210, 252
BtrI CACGTC 2 cut(s) 139, 676
BtsCI GGATG 2 cut(s) 111, 373
BtsI GCAGTG 1 cut(s) 465
BtsIMutI CAGTG 4 cut(s) 465, 606, 656, 696
Cac8I GCNNGC 3 cut(s) 31, 794, 933
CciI TCATGA 2 cut(s) 337, 859
CfoI GCGC 1 cut(s) 937
Cfr10I RCCGGY 2 cut(s) 29, 74
Cfr13I GGNCC 6 cut(s) 7, 150, 151, 547, 837, 838
ClaI ATCGAT 2 cut(s) 210, 252
CseI GACGC 1 cut(s) 44
Csp6I GTAC 3 cut(s) 24, 241, 383
CviAII CATG 5 cut(s) 338, 344, 608, 860, 938
CviQI GTAC 3 cut(s) 24, 241, 383
DdeI CTNAG 3 cut(s) 699, 906, 958
DpnI GATC 7 cut(s) 52, 62, 126, 209, 251, 738, 772
DpnII GATC 7 cut(s) 50, 60, 124, 207, 249, 736, 770
Eco147I AGGCCT 1 cut(s) 579
Eco24I GRGCYC 2 cut(s) 154, 841
Eco47I GGWCC 1 cut(s) 547
Eco88I CYCGRG 1 cut(s) 147
EcoO109I RGGNCCY 2 cut(s) 151, 838
EcoRII CCWGG 1 cut(s) 9
EcoT22I ATGCAT 1 cut(s) 609
EcoT38I GRGCYC 2 cut(s) 154, 841
Esp3I CGTCTC 1 cut(s) 505
FaeI CATG 5 cut(s) 341, 347, 611, 863, 941
FaqI GGGAC 2 cut(s) 77, 938
FatI CATG 5 cut(s) 337, 343, 607, 859, 937
FauI CCCGC 1 cut(s) 903
Fnu4HI GCNGC 7 cut(s) 6, 39, 42, 45, 79, 284, 929
FokI GGATG 2 cut(s) 98, 380
FriOI GRGCYC 2 cut(s) 154, 841
Fsp4HI GCNGC 7 cut(s) 6, 39, 42, 45, 79, 284, 929
FspBI CTAG 2 cut(s) 312, 806
FspI TGCGCA 1 cut(s) 936
GlaI GCGC 1 cut(s) 936
GluI GCNGC 7 cut(s) 6, 39, 42, 45, 79, 284, 929
HaeIII GGCC 5 cut(s) 8, 152, 501, 579, 839
HapII CCGG 4 cut(s) 30, 75, 84, 220
HgaI GACGC 1 cut(s) 44
HhaI GCGC 1 cut(s) 937
Hin1I GRCGYC 1 cut(s) 36
Hin1II CATG 5 cut(s) 341, 347, 611, 863, 941
Hin6I GCGC 1 cut(s) 935
HinP1I GCGC 1 cut(s) 935
HinfI GANTC 7 cut(s) 18, 307, 320, 334, 856, 887, 951
HpaII CCGG 4 cut(s) 30, 75, 84, 220
HphI GGTGA 1 cut(s) 656
Hpy166II GTNNAC 3 cut(s) 294, 647, 784
Hpy188I TCNGA 4 cut(s) 124, 207, 483, 673
Hpy188III TCNNGA 4 cut(s) 338, 365, 806, 860
Hpy8I GTNNAC 3 cut(s) 294, 647, 784
Hpy99I CGWCG 4 cut(s) 38, 71, 202, 230
HpyAV CCTTC 3 cut(s) 170, 491, 908
HpyCH4III ACNGT 2 cut(s) 651, 801
HpyCH4IV ACGT 7 cut(s) 138, 197, 243, 266, 376, 675, 682
HpyCH4V TGCA 8 cut(s) 192, 353, 443, 458, 521, 572, 607, 928
HpyF10VI GCNNNNNNNGC 9 cut(s) 35, 44, 53, 78, 84, 141, 283, 604, 934
HpyF3I CTNAG 3 cut(s) 699, 906, 958
HpySE526I ACGT 7 cut(s) 138, 197, 243, 266, 376, 675, 682
Hsp92I GRCGYC 1 cut(s) 36
Hsp92II CATG 5 cut(s) 341, 347, 611, 863, 941
HspAI GCGC 1 cut(s) 935
KroI GCCGGC 1 cut(s) 29
KroNI GCCGGC 1 cut(s) 31
Kzo9I GATC 7 cut(s) 50, 60, 124, 207, 249, 736, 770
LmnI GCTCC 4 cut(s) 86, 92, 222, 274
Lsp1109I GCAGC 5 cut(s) 53, 56, 65, 270, 940
LweI GCATC 3 cut(s) 340, 581, 594
MaeI CTAG 2 cut(s) 312, 806
MaeII ACGT 7 cut(s) 138, 197, 243, 266, 376, 675, 682
MaeIII GTNAC 1 cut(s) 678
MalI GATC 7 cut(s) 52, 62, 126, 209, 251, 738, 772
MboI GATC 7 cut(s) 50, 60, 124, 207, 249, 736, 770
MboII GAAGA 4 cut(s) 55, 274, 626, 731
MflI RGATCY 1 cut(s) 736
MhlI GDGCHC 2 cut(s) 154, 841
MluCI AATT 4 cut(s) 437, 537, 584, 846
MlyI GAGTC 3 cut(s) 328, 850, 960
MmeI TCCRAC 1 cut(s) 714
MnlI CCTC 6 cut(s) 119, 130, 164, 538, 716, 813
Mph1103I ATGCAT 1 cut(s) 609
MreI CGCCGGCG 1 cut(s) 29
MroNI GCCGGC 1 cut(s) 29
MroXI GAANNNNTTC 1 cut(s) 266
MseI TTAA 2 cut(s) 101, 845
MslI CAYNNNNRTG 3 cut(s) 342, 348, 458
MspI CCGG 4 cut(s) 30, 75, 84, 220
MspR9I CCNGG 1 cut(s) 11
MvaI CCWGG 1 cut(s) 11
MwoI GCNNNNNNNGC 9 cut(s) 35, 44, 53, 78, 84, 141, 283, 604, 934
NaeI GCCGGC 1 cut(s) 31
NdeII GATC 7 cut(s) 50, 60, 124, 207, 249, 736, 770
NgoMIV GCCGGC 1 cut(s) 29
NlaIII CATG 5 cut(s) 341, 347, 611, 863, 941
NlaIV GGNNCC 4 cut(s) 73, 88, 152, 839
NmuCI GTSAC 1 cut(s) 678
NsbI TGCGCA 1 cut(s) 936
NsiI ATGCAT 1 cut(s) 609
NspI RCATGY 1 cut(s) 347
PagI TCATGA 2 cut(s) 337, 859
PceI AGGCCT 1 cut(s) 579
PcsI WCGNNNNNNNCGW 1 cut(s) 249
PdiI GCCGGC 1 cut(s) 31
PdmI GAANNNNTTC 1 cut(s) 266
PfeI GAWTC 4 cut(s) 18, 307, 320, 887
PkrI GCNGC 7 cut(s) 7, 40, 43, 46, 80, 285, 930
Ple19I CGATCG 1 cut(s) 53
PleI GAGTC 3 cut(s) 328, 850, 959
PpsI GAGTC 3 cut(s) 328, 850, 959
Ppu21I YACGTR 2 cut(s) 244, 377
PshBI ATTAAT 1 cut(s) 845
Psp6I CCWGG 1 cut(s) 9
PspGI CCWGG 1 cut(s) 9
PspN4I GGNNCC 4 cut(s) 73, 88, 152, 839
PspOMI GGGCCC 2 cut(s) 150, 837
PspPI GGNCC 6 cut(s) 7, 150, 151, 547, 837, 838
PsuI RGATCY 1 cut(s) 736
PvuI CGATCG 1 cut(s) 53
RsaI GTAC 3 cut(s) 25, 242, 384
RsaNI GTAC 3 cut(s) 24, 241, 383
RseI CAYNNNNRTG 3 cut(s) 342, 348, 458
SaqAI TTAA 2 cut(s) 101, 845
SatI GCNGC 7 cut(s) 6, 39, 42, 45, 79, 284, 929
Sau3AI GATC 7 cut(s) 50, 60, 124, 207, 249, 736, 770
Sau96I GGNCC 6 cut(s) 7, 150, 151, 547, 837, 838
SchI GAGTC 3 cut(s) 328, 850, 960
ScrFI CCNGG 1 cut(s) 11
SduI GDGCHC 2 cut(s) 154, 841
SfaNI GCATC 3 cut(s) 340, 581, 594
SfcI CTRYAG 1 cut(s) 184
SgrAI CRCCGGYG 1 cut(s) 29
SinI GGWCC 1 cut(s) 547
SmiMI CAYNNNNRTG 3 cut(s) 342, 348, 458
Sse9I AATT 4 cut(s) 437, 537, 584, 846
SseBI AGGCCT 1 cut(s) 579
SsiI CCGC 5 cut(s) 5, 27, 39, 286, 910
SspMI CTAG 2 cut(s) 312, 806
StuI AGGCCT 1 cut(s) 579
StyD4I CCNGG 1 cut(s) 9
TaaI ACNGT 2 cut(s) 651, 801
TaiI ACGT 7 cut(s) 141, 200, 246, 269, 379, 678, 685
TaqI TCGA 4 cut(s) 210, 252, 528, 655
TasI AATT 4 cut(s) 437, 537, 584, 846
TatI WGTACW 1 cut(s) 382
TauI GCSGC 2 cut(s) 8, 41
TfiI GAWTC 4 cut(s) 18, 307, 320, 887
Tru1I TTAA 2 cut(s) 101, 845
Tru9I TTAA 2 cut(s) 101, 845
TscAI CASTG 4 cut(s) 465, 606, 656, 703
TseFI GTSAC 1 cut(s) 678
TseI GCWGC 5 cut(s) 41, 44, 78, 283, 928
Tsp45I GTSAC 1 cut(s) 678
TspDTI ATGAA 2 cut(s) 354, 900
TspGWI ACGGA 2 cut(s) 30, 79
TspRI CASTG 4 cut(s) 465, 606, 656, 703
VpaK11BI GGWCC 1 cut(s) 547
VspI ATTAAT 1 cut(s) 845
XbaI TCTAGA 1 cut(s) 805
XceI RCATGY 1 cut(s) 347
XmnI GAANNNNTTC 1 cut(s) 266
XspI CTAG 2 cut(s) 312, 806
Zsp2I ATGCAT 1 cut(s) 609
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.