Rh5CG125700

Hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0009230
Physical Location & Seq
Forward (+)
10483576 .. 10488255
4680 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG125700.1

Sequence Viewer

Length: 921 bp
ATGGCGGCCCAGGACGGAATCTGTACCGCCGGCGACGCCGCAGCCCGATCGGCTCTGATATTCGTCGGCACCGGCTGCTCCAGCTCCGTCCCCAACTTAATGTGCCTCATCCAGCCCTCTGATCCCCCCTGCCACGTCTGCTCTCAGGCCCTCTCCCTTCCCCCCGACCAAAACCCTAACTACAGGTGCAATACGTCGCTTCTGATCGATTATTGCCGGAGCGACGGCGAACACAGGTACGTGTTGATCGACGTGGGGAAGACGTTCAGGGAGCAAGTGCTGCGGTGGTTCACTCGCTATAAGATTCCTAGAGTTGATTCTATTATTTTGACTCATGAACATGCTGATGCAGTTCTTGGTCTGGATGACATACGTGCTGTACAGCCACATAGTTCCACAAATGACATTGAGCCCACTCCCATCTACCTAACTCAATTTGCAATGGAAAGCATTGCAGTGAAGTTCCCTTACTTGGTTCAGAAAAAACTTAAGGAAGGCCAAGAAGTGAGACGAGTTGCACAACTCGACTGGAAAATTATTGAGGACCATTGTGAAAAACCTTTTGTTGCATCAGGCCTACAATTTGTTCCTTTGCCAGTGATGCATGGGGAAGATTATGTCTGCTTGGGGTATCTATTTGGTGAACAGTGTCGAGTAGCTTATATATCTGACGTGTCACGTTTTCTTTCAAGCACAGAGCATTTGATTTCAAAAGATGGCGGTGGGCAGTTGGATCTTCTTATCTTGGACACATTATACAAGAATGGATCGCACAATGTTCACTTGTGCTTGCCACAGTCTCTAGAAGCTGTAAAGAGGATTTGTCCTAAACGGGCCCTATTAATTGGAATGACTCATCAGTTTGACCACCATAAGGACAATGAATCTCTTAGGGAATGGTCTAAGCGGTACGAATTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00440 map00440
Pfam Domains
Protein Families
Lactamase_B_2

Protein Analysis

306

Amino Acids

34.48

Weight (kDa)

6.1

Isoelectric Point (pI)

44.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Anti-Pycsar_Apyc1 PF23023 77 - 264 2.7e-06 Anti-Pycsar protein Apyc1
Lactamase_B_2 PF12706 79 - 283 5.1e-22 Beta-lactamase superfamily domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 68
AciI CCGC 6 cut(s) 5, 27, 39, 283, 720, 907
AclWI GGATC 3 cut(s) 116, 741, 775
AcyI GRCGYC 1 cut(s) 36
AfaI GTAC 4 cut(s) 25, 239, 381, 911
AfiI CCNNNNNNNGG 2 cut(s) 472, 874
AflII CTTAAG 1 cut(s) 488
AflIII ACRYGT 2 cut(s) 240, 672
AgsI TTSAA 2 cut(s) 690, 711
AjiI CACGTC 3 cut(s) 136, 253, 673
AjnI CCWGG 1 cut(s) 9
AluBI AGCT 3 cut(s) 84, 659, 809
AluI AGCT 3 cut(s) 84, 659, 809
Alw26I GTCTC 2 cut(s) 502, 804
AlwI GGATC 3 cut(s) 116, 741, 775
AoxI GGCC 5 cut(s) 6, 147, 496, 574, 834
ApaI GGGCCC 1 cut(s) 838
ApeKI GCWGC 3 cut(s) 41, 75, 280
AseI ATTAAT 1 cut(s) 842
Asp700I GAANNNNTTC 1 cut(s) 263
AspS9I GGNCC 5 cut(s) 7, 148, 544, 834, 835
AsuHPI GGTGA 1 cut(s) 653
AvaII GGWCC 1 cut(s) 544
BaeGI GKGCMC 1 cut(s) 838
BanI GGYRCC 1 cut(s) 68
BanII GRGCYC 2 cut(s) 414, 838
BarI GAAGNNNNNNTAC 2 cut(s) 452, 484
BbsI GAAGAC 1 cut(s) 266
BbvI GCAGC 3 cut(s) 53, 62, 267
BccI CCATC 2 cut(s) 428, 710
BceAI ACGGC 1 cut(s) 241
BcgI CGANNNNNNTGC 2 cut(s) 30, 64
BciT130I CCWGG 1 cut(s) 11
BcoDI GTCTC 2 cut(s) 502, 804
BfaI CTAG 2 cut(s) 309, 803
BfmI CTRYAG 1 cut(s) 181
BfrI CTTAAG 1 cut(s) 488
BglI GCCNNNNNGGC 1 cut(s) 50
BisI GCNGC 5 cut(s) 6, 39, 42, 76, 281
BlsI GCNGC 5 cut(s) 7, 40, 43, 77, 282
Bme1390I CCNGG 1 cut(s) 11
Bme18I GGWCC 1 cut(s) 544
BmgBI CACGTC 3 cut(s) 136, 253, 673
BmgT120I GGNCC 5 cut(s) 7, 148, 544, 834, 835
BmiI GGNNCC 2 cut(s) 70, 836
BmrFI CCNGG 1 cut(s) 11
BmsI GCATC 3 cut(s) 337, 578, 591
BpiI GAAGAC 1 cut(s) 266
BpmI CTGGAG 1 cut(s) 64
Bsa29I ATCGAT 1 cut(s) 207
BsaAI YACGTR 2 cut(s) 241, 374
BsaHI GRCGYC 1 cut(s) 36
BsaJI CCNNGG 1 cut(s) 9
Bsc4I CCNNNNNNNGG 2 cut(s) 472, 874
Bse118I RCCGGY 2 cut(s) 29, 71
Bse1I ACTGG 2 cut(s) 533, 596
Bse3DI GCAATG 2 cut(s) 447, 450
BseBI CCWGG 1 cut(s) 11
BseCI ATCGAT 1 cut(s) 207
BseDI CCNNGG 1 cut(s) 9
BseGI GGATG 2 cut(s) 108, 370
BseLI CCNNNNNNNGG 2 cut(s) 472, 874
BseMI GCAATG 2 cut(s) 447, 450
BseMII CTCAG 1 cut(s) 158
BseNI ACTGG 2 cut(s) 533, 596
BseSI GKGCMC 1 cut(s) 838
BseXI GCAGC 3 cut(s) 53, 62, 267
Bsh1285I CGRYCG 1 cut(s) 50
BshFI GGCC 5 cut(s) 8, 149, 498, 576, 836
BshNI GGYRCC 1 cut(s) 68
BshVI ATCGAT 1 cut(s) 207
BsiEI CGRYCG 1 cut(s) 50
BsiSI CCGG 3 cut(s) 30, 72, 217
BslFI GGGAC 1 cut(s) 74
BslI CCNNNNNNNGG 2 cut(s) 472, 874
BsmAI GTCTC 2 cut(s) 502, 804
BsmBI CGTCTC 1 cut(s) 502
BsmFI GGGAC 1 cut(s) 74
BsnI GGCC 5 cut(s) 8, 149, 498, 576, 836
Bsp120I GGGCCC 1 cut(s) 834
Bsp1286I GDGCHC 2 cut(s) 414, 838
Bsp1407I TGTACA 1 cut(s) 379
Bsp143I GATC 6 cut(s) 47, 121, 204, 246, 733, 767
BspACI CCGC 6 cut(s) 5, 27, 39, 283, 720, 907
BspANI GGCC 5 cut(s) 8, 149, 498, 576, 836
BspCNI CTCAG 1 cut(s) 157
BspDI ATCGAT 1 cut(s) 207
BspHI TCATGA 1 cut(s) 334
BspLI GGNNCC 2 cut(s) 70, 836
BspPI GGATC 3 cut(s) 116, 741, 775
BspT107I GGYRCC 1 cut(s) 68
BspTI CTTAAG 1 cut(s) 488
BsrDI GCAATG 2 cut(s) 447, 450
BsrFI RCCGGY 2 cut(s) 29, 71
BsrGI TGTACA 1 cut(s) 379
BsrI ACTGG 2 cut(s) 533, 596
BssAI RCCGGY 2 cut(s) 29, 71
BssECI CCNNGG 1 cut(s) 9
BssMI GATC 6 cut(s) 47, 121, 204, 246, 733, 767
BssNI GRCGYC 1 cut(s) 36
Bst2UI CCWGG 1 cut(s) 11
Bst4CI ACNGT 2 cut(s) 648, 798
BstACI GRCGYC 1 cut(s) 36
BstAFI CTTAAG 1 cut(s) 488
BstAPI GCANNNNNTGC 2 cut(s) 75, 280
BstAUI TGTACA 1 cut(s) 379
BstBAI YACGTR 2 cut(s) 241, 374
BstC8I GCNNGC 2 cut(s) 31, 791
BstDEI CTNAG 3 cut(s) 144, 890, 903
BstF5I GGATG 2 cut(s) 108, 370
BstKTI GATC 6 cut(s) 50, 124, 207, 249, 736, 770
BstMAI GTCTC 2 cut(s) 502, 804
BstMBI GATC 6 cut(s) 47, 121, 204, 246, 733, 767
BstMCI CGRYCG 1 cut(s) 50
BstMWI GCNNNNNNNGC 7 cut(s) 35, 50, 75, 81, 138, 280, 601
BstNI CCWGG 1 cut(s) 11
BstNSI RCATGY 1 cut(s) 344
BstSCI CCNGG 1 cut(s) 9
BstSFI CTRYAG 1 cut(s) 181
BstSLI GKGCMC 1 cut(s) 838
BstV1I GCAGC 3 cut(s) 53, 62, 267
BstV2I GAAGAC 1 cut(s) 266
BstX2I RGATCY 1 cut(s) 733
BstYI RGATCY 1 cut(s) 733
Bsu15I ATCGAT 1 cut(s) 207
BsuRI GGCC 5 cut(s) 8, 149, 498, 576, 836
BsuTUI ATCGAT 1 cut(s) 207
BtrI CACGTC 3 cut(s) 136, 253, 673
BtsCI GGATG 2 cut(s) 108, 370
BtsI GCAGTG 1 cut(s) 462
BtsIMutI CAGTG 3 cut(s) 462, 603, 653
Cac8I GCNNGC 2 cut(s) 31, 791
CciI TCATGA 1 cut(s) 334
Cfr10I RCCGGY 2 cut(s) 29, 71
Cfr13I GGNCC 5 cut(s) 7, 148, 544, 834, 835
ClaI ATCGAT 1 cut(s) 207
CseI GACGC 1 cut(s) 44
Csp6I GTAC 4 cut(s) 24, 238, 380, 910
CviAII CATG 3 cut(s) 335, 341, 605
CviQI GTAC 4 cut(s) 24, 238, 380, 910
DdeI CTNAG 3 cut(s) 144, 890, 903
DpnI GATC 6 cut(s) 49, 123, 206, 248, 735, 769
DpnII GATC 6 cut(s) 47, 121, 204, 246, 733, 767
Eco147I AGGCCT 1 cut(s) 576
Eco24I GRGCYC 2 cut(s) 414, 838
Eco47I GGWCC 1 cut(s) 544
EcoO109I RGGNCCY 2 cut(s) 148, 835
EcoRII CCWGG 1 cut(s) 9
EcoT22I ATGCAT 1 cut(s) 606
EcoT38I GRGCYC 2 cut(s) 414, 838
Esp3I CGTCTC 1 cut(s) 502
FaeI CATG 3 cut(s) 338, 344, 608
FaqI GGGAC 1 cut(s) 74
FatI CATG 3 cut(s) 334, 340, 604
Fnu4HI GCNGC 5 cut(s) 6, 39, 42, 76, 281
FokI GGATG 2 cut(s) 95, 377
FriOI GRGCYC 2 cut(s) 414, 838
Fsp4HI GCNGC 5 cut(s) 6, 39, 42, 76, 281
FspBI CTAG 2 cut(s) 309, 803
GluI GCNGC 5 cut(s) 6, 39, 42, 76, 281
GsuI CTGGAG 1 cut(s) 64
HaeIII GGCC 5 cut(s) 8, 149, 498, 576, 836
HapII CCGG 3 cut(s) 30, 72, 217
HgaI GACGC 1 cut(s) 44
Hin1I GRCGYC 1 cut(s) 36
Hin1II CATG 3 cut(s) 338, 344, 608
HinfI GANTC 6 cut(s) 18, 304, 317, 331, 853, 884
HpaII CCGG 3 cut(s) 30, 72, 217
HphI GGTGA 1 cut(s) 653
Hpy166II GTNNAC 3 cut(s) 291, 644, 781
Hpy188I TCNGA 5 cut(s) 57, 121, 204, 480, 670
Hpy188III TCNNGA 3 cut(s) 335, 362, 803
Hpy8I GTNNAC 3 cut(s) 291, 644, 781
Hpy99I CGWCG 5 cut(s) 38, 68, 199, 227, 254
HpyAV CCTTC 2 cut(s) 167, 488
HpyCH4III ACNGT 2 cut(s) 648, 798
HpyCH4IV ACGT 8 cut(s) 135, 194, 240, 252, 263, 373, 672, 679
HpyCH4V TGCA 7 cut(s) 189, 350, 440, 455, 518, 569, 604
HpyF10VI GCNNNNNNNGC 7 cut(s) 35, 50, 75, 81, 138, 280, 601
HpyF3I CTNAG 3 cut(s) 144, 890, 903
HpySE526I ACGT 8 cut(s) 135, 194, 240, 252, 263, 373, 672, 679
Hsp92I GRCGYC 1 cut(s) 36
Hsp92II CATG 3 cut(s) 338, 344, 608
KroI GCCGGC 1 cut(s) 29
KroNI GCCGGC 1 cut(s) 31
Kzo9I GATC 6 cut(s) 47, 121, 204, 246, 733, 767
LmnI GCTCC 4 cut(s) 83, 89, 219, 271
Lsp1109I GCAGC 3 cut(s) 53, 62, 267
LweI GCATC 3 cut(s) 337, 578, 591
MaeI CTAG 2 cut(s) 309, 803
MaeII ACGT 8 cut(s) 135, 194, 240, 252, 263, 373, 672, 679
MaeIII GTNAC 1 cut(s) 675
MalI GATC 6 cut(s) 49, 123, 206, 248, 735, 769
MboI GATC 6 cut(s) 47, 121, 204, 246, 733, 767
MboII GAAGA 3 cut(s) 271, 623, 728
MflI RGATCY 1 cut(s) 733
MhlI GDGCHC 2 cut(s) 414, 838
MluCI AATT 5 cut(s) 434, 534, 581, 843, 914
MlyI GAGTC 2 cut(s) 325, 847
MmeI TCCRAC 1 cut(s) 711
MnlI CCTC 5 cut(s) 116, 127, 161, 535, 810
Mph1103I ATGCAT 1 cut(s) 606
MreI CGCCGGCG 1 cut(s) 29
MroNI GCCGGC 1 cut(s) 29
MroXI GAANNNNTTC 1 cut(s) 263
MseI TTAA 3 cut(s) 98, 489, 842
MslI CAYNNNNRTG 3 cut(s) 339, 345, 455
MspCI CTTAAG 1 cut(s) 488
MspI CCGG 3 cut(s) 30, 72, 217
MspR9I CCNGG 1 cut(s) 11
MvaI CCWGG 1 cut(s) 11
MwoI GCNNNNNNNGC 7 cut(s) 35, 50, 75, 81, 138, 280, 601
NaeI GCCGGC 1 cut(s) 31
NdeII GATC 6 cut(s) 47, 121, 204, 246, 733, 767
NgoMIV GCCGGC 1 cut(s) 29
NlaIII CATG 3 cut(s) 338, 344, 608
NlaIV GGNNCC 2 cut(s) 70, 836
NmuCI GTSAC 1 cut(s) 675
NsiI ATGCAT 1 cut(s) 606
NspI RCATGY 1 cut(s) 344
PagI TCATGA 1 cut(s) 334
PceI AGGCCT 1 cut(s) 576
PcsI WCGNNNNNNNCGW 1 cut(s) 246
PdiI GCCGGC 1 cut(s) 31
PdmI GAANNNNTTC 1 cut(s) 263
PfeI GAWTC 4 cut(s) 18, 304, 317, 884
PkrI GCNGC 5 cut(s) 7, 40, 43, 77, 282
Ple19I CGATCG 1 cut(s) 50
PleI GAGTC 2 cut(s) 325, 847
PpsI GAGTC 2 cut(s) 325, 847
Ppu21I YACGTR 2 cut(s) 241, 374
PshBI ATTAAT 1 cut(s) 842
Psp6I CCWGG 1 cut(s) 9
PspGI CCWGG 1 cut(s) 9
PspN4I GGNNCC 2 cut(s) 70, 836
PspOMI GGGCCC 1 cut(s) 834
PspPI GGNCC 5 cut(s) 7, 148, 544, 834, 835
PsuI RGATCY 1 cut(s) 733
PvuI CGATCG 1 cut(s) 50
RsaI GTAC 4 cut(s) 25, 239, 381, 911
RsaNI GTAC 4 cut(s) 24, 238, 380, 910
RseI CAYNNNNRTG 3 cut(s) 339, 345, 455
SaqAI TTAA 3 cut(s) 98, 489, 842
SatI GCNGC 5 cut(s) 6, 39, 42, 76, 281
Sau3AI GATC 6 cut(s) 47, 121, 204, 246, 733, 767
Sau96I GGNCC 5 cut(s) 7, 148, 544, 834, 835
SchI GAGTC 2 cut(s) 325, 847
ScrFI CCNGG 1 cut(s) 11
SduI GDGCHC 2 cut(s) 414, 838
SfaNI GCATC 3 cut(s) 337, 578, 591
SfcI CTRYAG 1 cut(s) 181
SgrAI CRCCGGYG 1 cut(s) 29
SinI GGWCC 1 cut(s) 544
SmiMI CAYNNNNRTG 3 cut(s) 339, 345, 455
SmlI CTYRAG 1 cut(s) 488
SmoI CTYRAG 1 cut(s) 488
Sse9I AATT 5 cut(s) 434, 534, 581, 843, 914
SseBI AGGCCT 1 cut(s) 576
SsiI CCGC 6 cut(s) 5, 27, 39, 283, 720, 907
SspMI CTAG 2 cut(s) 309, 803
StuI AGGCCT 1 cut(s) 576
StyD4I CCNGG 1 cut(s) 9
TaaI ACNGT 2 cut(s) 648, 798
TaiI ACGT 8 cut(s) 138, 197, 243, 255, 266, 376, 675, 682
TaqI TCGA 4 cut(s) 207, 249, 525, 652
TasI AATT 5 cut(s) 434, 534, 581, 843, 914
TatI WGTACW 1 cut(s) 379
TauI GCSGC 2 cut(s) 8, 41
TfiI GAWTC 4 cut(s) 18, 304, 317, 884
Tru1I TTAA 3 cut(s) 98, 489, 842
Tru9I TTAA 3 cut(s) 98, 489, 842
TscAI CASTG 3 cut(s) 462, 603, 653
TseFI GTSAC 1 cut(s) 675
TseI GCWGC 3 cut(s) 41, 75, 280
Tsp45I GTSAC 1 cut(s) 675
TspDTI ATGAA 2 cut(s) 351, 897
TspGWI ACGGA 2 cut(s) 30, 76
TspRI CASTG 3 cut(s) 462, 603, 653
Vha464I CTTAAG 1 cut(s) 488
VpaK11BI GGWCC 1 cut(s) 544
VspI ATTAAT 1 cut(s) 842
XbaI TCTAGA 1 cut(s) 802
XceI RCATGY 1 cut(s) 344
XmnI GAANNNNTTC 1 cut(s) 263
XspI CTAG 2 cut(s) 309, 803
Zsp2I ATGCAT 1 cut(s) 606
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.