Rh5AG117200

Hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0009230
Physical Location & Seq
Forward (+)
10878720 .. 10883491
4772 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG117200.1

Sequence Viewer

Length: 816 bp
ATGGCGGCCCAGGACGGAATCTGTACCGCCGGCGACGCCGCAGCCCGATCGGCTCTGATCTTCGTCGGCACCGGCTGCTCCAGCTCCGTCCCCAACTTAAGGTGCCTTATCCAGCCCTCCGATCCCCCCTGCCACGTCTGCTCTCAGGCCCTCTCCCTTCCCCCCGACCAAAACCCTAACTACAGGTGCAATACGTCGCTTCTGATCGATTATTGCCGGAGCGACGGCGAACACAGGTACGTGTTGATCGATGTGGGGAAGACGTTCAGGGAGCAAGTGCTGCGGTGGTTCACTCGCTATAAGATTCCTAGAGTTGATTCTATTATTTTGACTCATGAACATGCTGATGCAGTTCTTGGTCTGGATGACATACGTGCTGTACAGCCACATAGTTCCACAAATAACATTGAGCCTACTCCCATCTACCTAACTCAATTTGCAATGGAAAGCATTGCAGTGAAGTTCCCTTACTTGGTTCAGAAAAAACTTAAGGAAGGCCAAGAAGTGAGACGAGTTGCACAACTCGACTGGAAAATTATTGAGGACCATTGTGAAAAACCTTTTGTTGCATCAGGCCTACAATTTGTTCCTTTGCCAGTGATGCATGGGGAAGATTACGTCTGCTTGGGGTATCTATTTGGTGAACAGTCTCGAGTAGCTTATATATCTGACGTGTCACGTTTTCTTTCAAGCACAGAGCATTTGATTTCAAAAGATGGCGGTGGGCAGTTGGATCTTCTTATCTTGGACACATTATACAAGAATGGATCGCACAATGTTCACTTGTGCTTGCCACAGGTTTGGTTTATTTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00440 map00440
Pfam Domains
Protein Families
Lactamase_B_2

Protein Analysis

271

Amino Acids

30.38

Weight (kDa)

5.91

Isoelectric Point (pI)

47.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Anti-Pycsar_Apyc1 PF23023 77 - 232 1.1e-06 Anti-Pycsar protein Apyc1
Lactamase_B_2 PF12706 79 - 262 3.1e-18 Beta-lactamase superfamily domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 68, 102
AciI CCGC 5 cut(s) 5, 27, 39, 283, 720
AclWI GGATC 3 cut(s) 116, 741, 775
AcyI GRCGYC 1 cut(s) 36
AfaI GTAC 3 cut(s) 25, 239, 381
AfiI CCNNNNNNNGG 2 cut(s) 99, 472
AflII CTTAAG 2 cut(s) 97, 488
AflIII ACRYGT 2 cut(s) 240, 672
AgsI TTSAA 2 cut(s) 690, 711
AjiI CACGTC 2 cut(s) 136, 673
AjnI CCWGG 1 cut(s) 9
AluBI AGCT 2 cut(s) 84, 659
AluI AGCT 2 cut(s) 84, 659
Alw26I GTCTC 2 cut(s) 502, 654
AlwI GGATC 3 cut(s) 116, 741, 775
Ama87I CYCGRG 1 cut(s) 651
AoxI GGCC 4 cut(s) 6, 147, 496, 574
ApeKI GCWGC 3 cut(s) 41, 75, 280
Asp700I GAANNNNTTC 1 cut(s) 263
AspS9I GGNCC 3 cut(s) 7, 148, 544
AsuHPI GGTGA 1 cut(s) 653
AvaI CYCGRG 1 cut(s) 651
AvaII GGWCC 1 cut(s) 544
BanI GGYRCC 2 cut(s) 68, 102
BarI GAAGNNNNNNTAC 2 cut(s) 452, 484
BbsI GAAGAC 1 cut(s) 266
BbvI GCAGC 3 cut(s) 53, 62, 267
BccI CCATC 2 cut(s) 428, 710
BceAI ACGGC 1 cut(s) 241
BcgI CGANNNNNNTGC 2 cut(s) 30, 64
BciT130I CCWGG 1 cut(s) 11
BcoDI GTCTC 2 cut(s) 502, 654
BfaI CTAG 1 cut(s) 309
BfmI CTRYAG 1 cut(s) 181
BfrI CTTAAG 2 cut(s) 97, 488
BglI GCCNNNNNGGC 1 cut(s) 50
BisI GCNGC 5 cut(s) 6, 39, 42, 76, 281
BlsI GCNGC 5 cut(s) 7, 40, 43, 77, 282
Bme1390I CCNGG 1 cut(s) 11
Bme18I GGWCC 1 cut(s) 544
BmeT110I CYCGRG 1 cut(s) 651
BmgBI CACGTC 2 cut(s) 136, 673
BmgT120I GGNCC 3 cut(s) 7, 148, 544
BmiI GGNNCC 2 cut(s) 70, 104
BmrFI CCNGG 1 cut(s) 11
BmsI GCATC 3 cut(s) 337, 578, 591
BpiI GAAGAC 1 cut(s) 266
BpmI CTGGAG 1 cut(s) 64
Bsa29I ATCGAT 2 cut(s) 207, 249
BsaAI YACGTR 2 cut(s) 241, 374
BsaHI GRCGYC 1 cut(s) 36
BsaJI CCNNGG 1 cut(s) 9
Bsc4I CCNNNNNNNGG 2 cut(s) 99, 472
Bse118I RCCGGY 2 cut(s) 29, 71
Bse1I ACTGG 2 cut(s) 533, 596
Bse3DI GCAATG 2 cut(s) 447, 450
BseBI CCWGG 1 cut(s) 11
BseCI ATCGAT 2 cut(s) 207, 249
BseDI CCNNGG 1 cut(s) 9
BseGI GGATG 1 cut(s) 370
BseLI CCNNNNNNNGG 2 cut(s) 99, 472
BseMI GCAATG 2 cut(s) 447, 450
BseMII CTCAG 1 cut(s) 158
BseNI ACTGG 2 cut(s) 533, 596
BseXI GCAGC 3 cut(s) 53, 62, 267
Bsh1285I CGRYCG 1 cut(s) 50
BshFI GGCC 4 cut(s) 8, 149, 498, 576
BshNI GGYRCC 2 cut(s) 68, 102
BshVI ATCGAT 2 cut(s) 207, 249
BsiEI CGRYCG 1 cut(s) 50
BsiHKCI CYCGRG 1 cut(s) 651
BsiSI CCGG 3 cut(s) 30, 72, 217
BslFI GGGAC 1 cut(s) 74
BslI CCNNNNNNNGG 2 cut(s) 99, 472
BsmAI GTCTC 2 cut(s) 502, 654
BsmBI CGTCTC 1 cut(s) 502
BsmFI GGGAC 1 cut(s) 74
BsnI GGCC 4 cut(s) 8, 149, 498, 576
BsoBI CYCGRG 1 cut(s) 651
Bsp1407I TGTACA 1 cut(s) 379
Bsp143I GATC 7 cut(s) 47, 57, 121, 204, 246, 733, 767
BspACI CCGC 5 cut(s) 5, 27, 39, 283, 720
BspANI GGCC 4 cut(s) 8, 149, 498, 576
BspCNI CTCAG 1 cut(s) 157
BspDI ATCGAT 2 cut(s) 207, 249
BspHI TCATGA 1 cut(s) 334
BspLI GGNNCC 2 cut(s) 70, 104
BspPI GGATC 3 cut(s) 116, 741, 775
BspT107I GGYRCC 2 cut(s) 68, 102
BspTI CTTAAG 2 cut(s) 97, 488
BsrDI GCAATG 2 cut(s) 447, 450
BsrFI RCCGGY 2 cut(s) 29, 71
BsrGI TGTACA 1 cut(s) 379
BsrI ACTGG 2 cut(s) 533, 596
BssAI RCCGGY 2 cut(s) 29, 71
BssECI CCNNGG 1 cut(s) 9
BssMI GATC 7 cut(s) 47, 57, 121, 204, 246, 733, 767
BssNI GRCGYC 1 cut(s) 36
Bst2UI CCWGG 1 cut(s) 11
Bst4CI ACNGT 1 cut(s) 648
BstACI GRCGYC 1 cut(s) 36
BstAFI CTTAAG 2 cut(s) 97, 488
BstAPI GCANNNNNTGC 2 cut(s) 75, 280
BstAUI TGTACA 1 cut(s) 379
BstBAI YACGTR 2 cut(s) 241, 374
BstC8I GCNNGC 2 cut(s) 31, 791
BstDEI CTNAG 1 cut(s) 144
BstF5I GGATG 1 cut(s) 370
BstKTI GATC 7 cut(s) 50, 60, 124, 207, 249, 736, 770
BstMAI GTCTC 2 cut(s) 502, 654
BstMBI GATC 7 cut(s) 47, 57, 121, 204, 246, 733, 767
BstMCI CGRYCG 1 cut(s) 50
BstMWI GCNNNNNNNGC 7 cut(s) 35, 50, 75, 81, 138, 280, 601
BstNI CCWGG 1 cut(s) 11
BstNSI RCATGY 1 cut(s) 344
BstSCI CCNGG 1 cut(s) 9
BstSFI CTRYAG 1 cut(s) 181
BstV1I GCAGC 3 cut(s) 53, 62, 267
BstV2I GAAGAC 1 cut(s) 266
BstX2I RGATCY 1 cut(s) 733
BstXI CCANNNNNNTGG 1 cut(s) 801
BstYI RGATCY 1 cut(s) 733
Bsu15I ATCGAT 2 cut(s) 207, 249
BsuRI GGCC 4 cut(s) 8, 149, 498, 576
BsuTUI ATCGAT 2 cut(s) 207, 249
BtrI CACGTC 2 cut(s) 136, 673
BtsCI GGATG 1 cut(s) 370
BtsI GCAGTG 1 cut(s) 462
BtsIMutI CAGTG 2 cut(s) 462, 603
Cac8I GCNNGC 2 cut(s) 31, 791
CciI TCATGA 1 cut(s) 334
Cfr10I RCCGGY 2 cut(s) 29, 71
Cfr13I GGNCC 3 cut(s) 7, 148, 544
ClaI ATCGAT 2 cut(s) 207, 249
CseI GACGC 1 cut(s) 44
Csp6I GTAC 3 cut(s) 24, 238, 380
CviAII CATG 3 cut(s) 335, 341, 605
CviQI GTAC 3 cut(s) 24, 238, 380
DdeI CTNAG 1 cut(s) 144
DpnI GATC 7 cut(s) 49, 59, 123, 206, 248, 735, 769
DpnII GATC 7 cut(s) 47, 57, 121, 204, 246, 733, 767
Eco147I AGGCCT 1 cut(s) 576
Eco47I GGWCC 1 cut(s) 544
Eco88I CYCGRG 1 cut(s) 651
EcoO109I RGGNCCY 1 cut(s) 148
EcoRII CCWGG 1 cut(s) 9
EcoT22I ATGCAT 1 cut(s) 606
Esp3I CGTCTC 1 cut(s) 502
FaeI CATG 3 cut(s) 338, 344, 608
FaiI YATR 9 cut(s) 300, 336, 342, 371, 390, 606, 663, 665, 757
FaqI GGGAC 1 cut(s) 74
FatI CATG 3 cut(s) 334, 340, 604
Fnu4HI GCNGC 5 cut(s) 6, 39, 42, 76, 281
FokI GGATG 1 cut(s) 377
Fsp4HI GCNGC 5 cut(s) 6, 39, 42, 76, 281
FspBI CTAG 1 cut(s) 309
GluI GCNGC 5 cut(s) 6, 39, 42, 76, 281
GsuI CTGGAG 1 cut(s) 64
HaeIII GGCC 4 cut(s) 8, 149, 498, 576
HapII CCGG 3 cut(s) 30, 72, 217
HgaI GACGC 1 cut(s) 44
Hin1I GRCGYC 1 cut(s) 36
Hin1II CATG 3 cut(s) 338, 344, 608
HinfI GANTC 4 cut(s) 18, 304, 317, 331
HpaII CCGG 3 cut(s) 30, 72, 217
HphI GGTGA 1 cut(s) 653
Hpy166II GTNNAC 3 cut(s) 291, 644, 781
Hpy188I TCNGA 5 cut(s) 57, 121, 204, 480, 670
Hpy188III TCNNGA 3 cut(s) 335, 362, 651
Hpy8I GTNNAC 3 cut(s) 291, 644, 781
Hpy99I CGWCG 4 cut(s) 38, 68, 199, 227
HpyAV CCTTC 2 cut(s) 167, 488
HpyCH4III ACNGT 1 cut(s) 648
HpyCH4IV ACGT 8 cut(s) 135, 194, 240, 263, 373, 618, 672, 679
HpyCH4V TGCA 7 cut(s) 189, 350, 440, 455, 518, 569, 604
HpyF10VI GCNNNNNNNGC 7 cut(s) 35, 50, 75, 81, 138, 280, 601
HpyF3I CTNAG 1 cut(s) 144
HpySE526I ACGT 8 cut(s) 135, 194, 240, 263, 373, 618, 672, 679
Hsp92I GRCGYC 1 cut(s) 36
Hsp92II CATG 3 cut(s) 338, 344, 608
KroI GCCGGC 1 cut(s) 29
KroNI GCCGGC 1 cut(s) 31
Kzo9I GATC 7 cut(s) 47, 57, 121, 204, 246, 733, 767
LmnI GCTCC 4 cut(s) 83, 89, 219, 271
Lsp1109I GCAGC 3 cut(s) 53, 62, 267
LweI GCATC 3 cut(s) 337, 578, 591
MaeI CTAG 1 cut(s) 309
MaeII ACGT 8 cut(s) 135, 194, 240, 263, 373, 618, 672, 679
MaeIII GTNAC 1 cut(s) 675
MalI GATC 7 cut(s) 49, 59, 123, 206, 248, 735, 769
MboI GATC 7 cut(s) 47, 57, 121, 204, 246, 733, 767
MboII GAAGA 4 cut(s) 52, 271, 623, 728
MflI RGATCY 1 cut(s) 733
MluCI AATT 3 cut(s) 434, 534, 581
MlyI GAGTC 1 cut(s) 325
MmeI TCCRAC 1 cut(s) 711
MnlI CCTC 3 cut(s) 127, 161, 535
Mph1103I ATGCAT 1 cut(s) 606
MreI CGCCGGCG 1 cut(s) 29
MroNI GCCGGC 1 cut(s) 29
MroXI GAANNNNTTC 1 cut(s) 263
MseI TTAA 2 cut(s) 98, 489
MslI CAYNNNNRTG 3 cut(s) 339, 345, 455
MspCI CTTAAG 2 cut(s) 97, 488
MspI CCGG 3 cut(s) 30, 72, 217
MspR9I CCNGG 1 cut(s) 11
MvaI CCWGG 1 cut(s) 11
MwoI GCNNNNNNNGC 7 cut(s) 35, 50, 75, 81, 138, 280, 601
NaeI GCCGGC 1 cut(s) 31
NdeII GATC 7 cut(s) 47, 57, 121, 204, 246, 733, 767
NgoMIV GCCGGC 1 cut(s) 29
NlaIII CATG 3 cut(s) 338, 344, 608
NlaIV GGNNCC 2 cut(s) 70, 104
NmuCI GTSAC 1 cut(s) 675
NsiI ATGCAT 1 cut(s) 606
NspI RCATGY 1 cut(s) 344
PaeR7I CTCGAG 1 cut(s) 651
PagI TCATGA 1 cut(s) 334
PceI AGGCCT 1 cut(s) 576
PcsI WCGNNNNNNNCGW 1 cut(s) 246
PdiI GCCGGC 1 cut(s) 31
PdmI GAANNNNTTC 1 cut(s) 263
PfeI GAWTC 3 cut(s) 18, 304, 317
PkrI GCNGC 5 cut(s) 7, 40, 43, 77, 282
Ple19I CGATCG 1 cut(s) 50
PleI GAGTC 1 cut(s) 325
PpsI GAGTC 1 cut(s) 325
Ppu21I YACGTR 2 cut(s) 241, 374
Psp6I CCWGG 1 cut(s) 9
PspGI CCWGG 1 cut(s) 9
PspN4I GGNNCC 2 cut(s) 70, 104
PspPI GGNCC 3 cut(s) 7, 148, 544
PsuI RGATCY 1 cut(s) 733
PvuI CGATCG 1 cut(s) 50
RsaI GTAC 3 cut(s) 25, 239, 381
RsaNI GTAC 3 cut(s) 24, 238, 380
RseI CAYNNNNRTG 3 cut(s) 339, 345, 455
SaqAI TTAA 2 cut(s) 98, 489
SatI GCNGC 5 cut(s) 6, 39, 42, 76, 281
Sau3AI GATC 7 cut(s) 47, 57, 121, 204, 246, 733, 767
Sau96I GGNCC 3 cut(s) 7, 148, 544
SchI GAGTC 1 cut(s) 325
ScrFI CCNGG 1 cut(s) 11
SfaNI GCATC 3 cut(s) 337, 578, 591
SfcI CTRYAG 1 cut(s) 181
Sfr274I CTCGAG 1 cut(s) 651
SgrAI CRCCGGYG 1 cut(s) 29
SinI GGWCC 1 cut(s) 544
SlaI CTCGAG 1 cut(s) 651
SmiMI CAYNNNNRTG 3 cut(s) 339, 345, 455
SmlI CTYRAG 3 cut(s) 97, 488, 651
SmoI CTYRAG 3 cut(s) 97, 488, 651
Sse9I AATT 3 cut(s) 434, 534, 581
SseBI AGGCCT 1 cut(s) 576
SsiI CCGC 5 cut(s) 5, 27, 39, 283, 720
SspMI CTAG 1 cut(s) 309
StuI AGGCCT 1 cut(s) 576
StyD4I CCNGG 1 cut(s) 9
TaaI ACNGT 1 cut(s) 648
TaiI ACGT 8 cut(s) 138, 197, 243, 266, 376, 621, 675, 682
TaqI TCGA 4 cut(s) 207, 249, 525, 652
TasI AATT 3 cut(s) 434, 534, 581
TatI WGTACW 1 cut(s) 379
TauI GCSGC 2 cut(s) 8, 41
TfiI GAWTC 3 cut(s) 18, 304, 317
Tru1I TTAA 2 cut(s) 98, 489
Tru9I TTAA 2 cut(s) 98, 489
TscAI CASTG 2 cut(s) 462, 603
TseFI GTSAC 1 cut(s) 675
TseI GCWGC 3 cut(s) 41, 75, 280
Tsp45I GTSAC 1 cut(s) 675
TspDTI ATGAA 1 cut(s) 351
TspGWI ACGGA 2 cut(s) 30, 76
TspRI CASTG 2 cut(s) 462, 603
Vha464I CTTAAG 2 cut(s) 97, 488
VpaK11BI GGWCC 1 cut(s) 544
XceI RCATGY 1 cut(s) 344
XhoI CTCGAG 1 cut(s) 651
XmnI GAANNNNTTC 1 cut(s) 263
XspI CTAG 1 cut(s) 309
Zsp2I ATGCAT 1 cut(s) 606
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.