Rroxscaffold_1G00061650

Hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0009230
Physical Location & Seq
Reverse (-)
83744108 .. 83748717
4610 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00061650.1

Sequence Viewer

Length: 882 bp
ATGGCGGCCCAGGACGGAATCTGTACCGCCGGCGACGCCGCAGCCCGATCGGCTCTGATCTTCGTCGGCACCGGCTGCTCCAGCTCCGTCCCCAACTTAATGTGCAATACGTCGCTTCTGATCGATTATTGCCGGAGCGACGGCGAACACAGGTACGTGTTGATCGATGTGGGGAAGACGTTCAGGGAGCAAGTGCTGCGGTGGTTCACTCGCTATAAGATTCCTAGAGTTGATTCTATTATTTTGACTCATGAACATGCTGATGCAGTTCTTGGTCTGGATGACATACGTGCTGTACAGCCATATAGTTCCACAAATGACATTGAGCCTACTCCCATCTACCTAACTCAATTTGCAATGGAAAGCATTGCAGTGAAGTTCCCTTACTTGGTTCAGAAAAAACGTAAGGAAGGCCAAGAAGTGAGACGAGTTGCACAACTCGACTGGAGAATTATTGAGGACCATTGTGAAAAACCTTTTGTTGCATCAGGCCTACAATTTGTTCCTTTGCCAGTGATGCATGGGGAAGATTATGTCTGCTTGGGGTATCTATTTGGTGAACAGTGTCGAGTAGCTTATATATCTGACGTGTCACGTTTTCTTTCAAGCACAGAGCATTTGATTTCAAAAGATGGCGGTGGGCAGTTGGATCTTCTTATCTTGGACACATTATACAAGAATGGATCGCACAATGTTCACTTGTGCTTGCCACAGTCTCTAGAAGCTGTAAAGAGGATTTGTCCTAAACGGGCCCTATTAATTGGAATGACTCATGAGTTTGACCACCATAAGGACAATGAATCTCTTATGGAATGGTCTAAGCGGGAAGGGATACCTGTGCAGCTTGCGCATGATGGTTTGAGAGTCCCCTTAGACCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00440 map00440
Pfam Domains
Protein Families
Lactamase_B_2

Protein Analysis

293

Amino Acids

33.0

Weight (kDa)

5.94

Isoelectric Point (pI)

45.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Anti-Pycsar_Apyc1 PF23023 19 - 259 1.4e-09 Anti-Pycsar protein Apyc1
Lactamase_B_2 PF12706 51 - 255 2.8e-22 Beta-lactamase superfamily domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 849
AccB1I GGYRCC 1 cut(s) 68
AciI CCGC 6 cut(s) 5, 27, 39, 199, 636, 823
AclWI GGATC 2 cut(s) 657, 691
AcyI GRCGYC 1 cut(s) 36
AfaI GTAC 3 cut(s) 25, 155, 297
AfiI CCNNNNNNNGG 2 cut(s) 388, 790
AflIII ACRYGT 2 cut(s) 156, 588
AgsI TTSAA 2 cut(s) 606, 627
AjiI CACGTC 1 cut(s) 589
AjnI CCWGG 1 cut(s) 9
AluBI AGCT 4 cut(s) 84, 575, 725, 844
AluI AGCT 4 cut(s) 84, 575, 725, 844
Alw26I GTCTC 2 cut(s) 418, 720
AlwI GGATC 2 cut(s) 657, 691
AoxI GGCC 4 cut(s) 6, 412, 490, 750
ApaI GGGCCC 1 cut(s) 754
ApeKI GCWGC 4 cut(s) 41, 75, 196, 841
AseI ATTAAT 1 cut(s) 758
Asp700I GAANNNNTTC 1 cut(s) 179
AspLEI GCGC 1 cut(s) 850
AspS9I GGNCC 4 cut(s) 7, 460, 750, 751
AsuHPI GGTGA 1 cut(s) 569
AvaII GGWCC 1 cut(s) 460
BaeGI GKGCMC 1 cut(s) 754
BanI GGYRCC 1 cut(s) 68
BanII GRGCYC 1 cut(s) 754
BarI GAAGNNNNNNTAC 2 cut(s) 368, 400
BbsI GAAGAC 1 cut(s) 182
BbvI GCAGC 4 cut(s) 53, 62, 183, 853
BccI CCATC 3 cut(s) 344, 626, 848
BceAI ACGGC 1 cut(s) 157
BcgI CGANNNNNNTGC 2 cut(s) 30, 64
BciT130I CCWGG 1 cut(s) 11
BciVI GTATCC 1 cut(s) 825
BcoDI GTCTC 2 cut(s) 418, 720
BfaI CTAG 2 cut(s) 225, 719
BfuI GTATCC 1 cut(s) 825
BglI GCCNNNNNGGC 1 cut(s) 50
BisI GCNGC 6 cut(s) 6, 39, 42, 76, 197, 842
BlsI GCNGC 6 cut(s) 7, 40, 43, 77, 198, 843
Bme1390I CCNGG 1 cut(s) 11
Bme18I GGWCC 1 cut(s) 460
BmgBI CACGTC 1 cut(s) 589
BmgT120I GGNCC 4 cut(s) 7, 460, 750, 751
BmiI GGNNCC 2 cut(s) 70, 752
BmrFI CCNGG 1 cut(s) 11
BmsI GCATC 3 cut(s) 253, 494, 507
BpiI GAAGAC 1 cut(s) 182
BpmI CTGGAG 2 cut(s) 64, 466
Bsa29I ATCGAT 2 cut(s) 123, 165
BsaAI YACGTR 2 cut(s) 157, 290
BsaHI GRCGYC 1 cut(s) 36
BsaJI CCNNGG 1 cut(s) 9
Bsc4I CCNNNNNNNGG 2 cut(s) 388, 790
Bse118I RCCGGY 2 cut(s) 29, 71
Bse1I ACTGG 2 cut(s) 449, 512
Bse3DI GCAATG 2 cut(s) 363, 366
BseBI CCWGG 1 cut(s) 11
BseCI ATCGAT 2 cut(s) 123, 165
BseDI CCNNGG 1 cut(s) 9
BseGI GGATG 1 cut(s) 286
BseLI CCNNNNNNNGG 2 cut(s) 388, 790
BseMI GCAATG 2 cut(s) 363, 366
BseNI ACTGG 2 cut(s) 449, 512
BseSI GKGCMC 1 cut(s) 754
BseXI GCAGC 4 cut(s) 53, 62, 183, 853
BsgI GTGCAG 1 cut(s) 860
Bsh1285I CGRYCG 1 cut(s) 50
BshFI GGCC 4 cut(s) 8, 414, 492, 752
BshNI GGYRCC 1 cut(s) 68
BshVI ATCGAT 2 cut(s) 123, 165
BsiEI CGRYCG 1 cut(s) 50
BsiSI CCGG 3 cut(s) 30, 72, 133
BslFI GGGAC 2 cut(s) 74, 851
BslI CCNNNNNNNGG 2 cut(s) 388, 790
BsmAI GTCTC 2 cut(s) 418, 720
BsmBI CGTCTC 1 cut(s) 418
BsmFI GGGAC 2 cut(s) 74, 851
BsnI GGCC 4 cut(s) 8, 414, 492, 752
Bsp120I GGGCCC 1 cut(s) 750
Bsp1286I GDGCHC 1 cut(s) 754
Bsp1407I TGTACA 1 cut(s) 295
Bsp143I GATC 6 cut(s) 47, 57, 120, 162, 649, 683
BspACI CCGC 6 cut(s) 5, 27, 39, 199, 636, 823
BspANI GGCC 4 cut(s) 8, 414, 492, 752
BspDI ATCGAT 2 cut(s) 123, 165
BspHI TCATGA 2 cut(s) 250, 772
BspLI GGNNCC 2 cut(s) 70, 752
BspPI GGATC 2 cut(s) 657, 691
BspT107I GGYRCC 1 cut(s) 68
BsrDI GCAATG 2 cut(s) 363, 366
BsrFI RCCGGY 2 cut(s) 29, 71
BsrGI TGTACA 1 cut(s) 295
BsrI ACTGG 2 cut(s) 449, 512
BssAI RCCGGY 2 cut(s) 29, 71
BssECI CCNNGG 1 cut(s) 9
BssMI GATC 6 cut(s) 47, 57, 120, 162, 649, 683
BssNI GRCGYC 1 cut(s) 36
Bst2UI CCWGG 1 cut(s) 11
Bst4CI ACNGT 2 cut(s) 564, 714
BstACI GRCGYC 1 cut(s) 36
BstAPI GCANNNNNTGC 2 cut(s) 75, 196
BstAUI TGTACA 1 cut(s) 295
BstBAI YACGTR 2 cut(s) 157, 290
BstC8I GCNNGC 3 cut(s) 31, 707, 846
BstDEI CTNAG 2 cut(s) 819, 871
BstF5I GGATG 1 cut(s) 286
BstHHI GCGC 1 cut(s) 850
BstKTI GATC 6 cut(s) 50, 60, 123, 165, 652, 686
BstMAI GTCTC 2 cut(s) 418, 720
BstMBI GATC 6 cut(s) 47, 57, 120, 162, 649, 683
BstMCI CGRYCG 1 cut(s) 50
BstMWI GCNNNNNNNGC 7 cut(s) 35, 50, 75, 81, 196, 517, 847
BstNI CCWGG 1 cut(s) 11
BstNSI RCATGY 1 cut(s) 260
BstSCI CCNGG 1 cut(s) 9
BstSLI GKGCMC 1 cut(s) 754
BstV1I GCAGC 4 cut(s) 53, 62, 183, 853
BstV2I GAAGAC 1 cut(s) 182
BstX2I RGATCY 1 cut(s) 649
BstYI RGATCY 1 cut(s) 649
Bsu15I ATCGAT 2 cut(s) 123, 165
BsuI GTATCC 1 cut(s) 825
BsuRI GGCC 4 cut(s) 8, 414, 492, 752
BsuTUI ATCGAT 2 cut(s) 123, 165
BtrI CACGTC 1 cut(s) 589
BtsCI GGATG 1 cut(s) 286
BtsI GCAGTG 1 cut(s) 378
BtsIMutI CAGTG 3 cut(s) 378, 519, 569
Cac8I GCNNGC 3 cut(s) 31, 707, 846
CciI TCATGA 2 cut(s) 250, 772
CfoI GCGC 1 cut(s) 850
Cfr10I RCCGGY 2 cut(s) 29, 71
Cfr13I GGNCC 4 cut(s) 7, 460, 750, 751
ClaI ATCGAT 2 cut(s) 123, 165
CseI GACGC 1 cut(s) 44
Csp6I GTAC 3 cut(s) 24, 154, 296
CviAII CATG 5 cut(s) 251, 257, 521, 773, 851
CviQI GTAC 3 cut(s) 24, 154, 296
DdeI CTNAG 2 cut(s) 819, 871
DpnI GATC 6 cut(s) 49, 59, 122, 164, 651, 685
DpnII GATC 6 cut(s) 47, 57, 120, 162, 649, 683
Eco147I AGGCCT 1 cut(s) 492
Eco24I GRGCYC 1 cut(s) 754
Eco47I GGWCC 1 cut(s) 460
EcoO109I RGGNCCY 1 cut(s) 751
EcoRII CCWGG 1 cut(s) 9
EcoT22I ATGCAT 1 cut(s) 522
EcoT38I GRGCYC 1 cut(s) 754
Esp3I CGTCTC 1 cut(s) 418
FaeI CATG 5 cut(s) 254, 260, 524, 776, 854
FaqI GGGAC 2 cut(s) 74, 851
FatI CATG 5 cut(s) 250, 256, 520, 772, 850
FauI CCCGC 1 cut(s) 816
Fnu4HI GCNGC 6 cut(s) 6, 39, 42, 76, 197, 842
FokI GGATG 1 cut(s) 293
FriOI GRGCYC 1 cut(s) 754
Fsp4HI GCNGC 6 cut(s) 6, 39, 42, 76, 197, 842
FspBI CTAG 2 cut(s) 225, 719
FspI TGCGCA 1 cut(s) 849
GlaI GCGC 1 cut(s) 849
GluI GCNGC 6 cut(s) 6, 39, 42, 76, 197, 842
GsuI CTGGAG 2 cut(s) 64, 466
HaeIII GGCC 4 cut(s) 8, 414, 492, 752
HapII CCGG 3 cut(s) 30, 72, 133
HgaI GACGC 1 cut(s) 44
HhaI GCGC 1 cut(s) 850
Hin1I GRCGYC 1 cut(s) 36
Hin1II CATG 5 cut(s) 254, 260, 524, 776, 854
Hin6I GCGC 1 cut(s) 848
HinP1I GCGC 1 cut(s) 848
HinfI GANTC 7 cut(s) 18, 220, 233, 247, 769, 800, 864
HpaII CCGG 3 cut(s) 30, 72, 133
HphI GGTGA 1 cut(s) 569
Hpy166II GTNNAC 3 cut(s) 207, 560, 697
Hpy188I TCNGA 4 cut(s) 57, 120, 396, 586
Hpy188III TCNNGA 4 cut(s) 251, 278, 719, 773
Hpy8I GTNNAC 3 cut(s) 207, 560, 697
Hpy99I CGWCG 4 cut(s) 38, 68, 115, 143
HpyAV CCTTC 2 cut(s) 404, 821
HpyCH4III ACNGT 2 cut(s) 564, 714
HpyCH4IV ACGT 7 cut(s) 110, 156, 179, 289, 403, 588, 595
HpyCH4V TGCA 8 cut(s) 105, 266, 356, 371, 434, 485, 520, 841
HpyF10VI GCNNNNNNNGC 7 cut(s) 35, 50, 75, 81, 196, 517, 847
HpyF3I CTNAG 2 cut(s) 819, 871
HpySE526I ACGT 7 cut(s) 110, 156, 179, 289, 403, 588, 595
Hsp92I GRCGYC 1 cut(s) 36
Hsp92II CATG 5 cut(s) 254, 260, 524, 776, 854
HspAI GCGC 1 cut(s) 848
KroI GCCGGC 1 cut(s) 29
KroNI GCCGGC 1 cut(s) 31
Kzo9I GATC 6 cut(s) 47, 57, 120, 162, 649, 683
LmnI GCTCC 4 cut(s) 83, 89, 135, 187
Lsp1109I GCAGC 4 cut(s) 53, 62, 183, 853
LweI GCATC 3 cut(s) 253, 494, 507
MaeI CTAG 2 cut(s) 225, 719
MaeII ACGT 7 cut(s) 110, 156, 179, 289, 403, 588, 595
MaeIII GTNAC 1 cut(s) 591
MalI GATC 6 cut(s) 49, 59, 122, 164, 651, 685
MboI GATC 6 cut(s) 47, 57, 120, 162, 649, 683
MboII GAAGA 4 cut(s) 52, 187, 539, 644
MflI RGATCY 1 cut(s) 649
MhlI GDGCHC 1 cut(s) 754
MluCI AATT 4 cut(s) 350, 450, 497, 759
MlyI GAGTC 3 cut(s) 241, 763, 873
MmeI TCCRAC 1 cut(s) 627
MnlI CCTC 2 cut(s) 451, 726
Mph1103I ATGCAT 1 cut(s) 522
MreI CGCCGGCG 1 cut(s) 29
MroNI GCCGGC 1 cut(s) 29
MroXI GAANNNNTTC 1 cut(s) 179
MseI TTAA 2 cut(s) 98, 758
MslI CAYNNNNRTG 3 cut(s) 255, 261, 371
MspI CCGG 3 cut(s) 30, 72, 133
MspR9I CCNGG 1 cut(s) 11
MvaI CCWGG 1 cut(s) 11
MwoI GCNNNNNNNGC 7 cut(s) 35, 50, 75, 81, 196, 517, 847
NaeI GCCGGC 1 cut(s) 31
NdeII GATC 6 cut(s) 47, 57, 120, 162, 649, 683
NgoMIV GCCGGC 1 cut(s) 29
NlaIII CATG 5 cut(s) 254, 260, 524, 776, 854
NlaIV GGNNCC 2 cut(s) 70, 752
NmuCI GTSAC 1 cut(s) 591
NsbI TGCGCA 1 cut(s) 849
NsiI ATGCAT 1 cut(s) 522
NspI RCATGY 1 cut(s) 260
PagI TCATGA 2 cut(s) 250, 772
PceI AGGCCT 1 cut(s) 492
PcsI WCGNNNNNNNCGW 1 cut(s) 162
PdiI GCCGGC 1 cut(s) 31
PdmI GAANNNNTTC 1 cut(s) 179
PfeI GAWTC 4 cut(s) 18, 220, 233, 800
PkrI GCNGC 6 cut(s) 7, 40, 43, 77, 198, 843
Ple19I CGATCG 1 cut(s) 50
PleI GAGTC 3 cut(s) 241, 763, 872
PpsI GAGTC 3 cut(s) 241, 763, 872
Ppu21I YACGTR 2 cut(s) 157, 290
PshBI ATTAAT 1 cut(s) 758
Psp6I CCWGG 1 cut(s) 9
PspGI CCWGG 1 cut(s) 9
PspN4I GGNNCC 2 cut(s) 70, 752
PspOMI GGGCCC 1 cut(s) 750
PspPI GGNCC 4 cut(s) 7, 460, 750, 751
PsuI RGATCY 1 cut(s) 649
PvuI CGATCG 1 cut(s) 50
RsaI GTAC 3 cut(s) 25, 155, 297
RsaNI GTAC 3 cut(s) 24, 154, 296
RseI CAYNNNNRTG 3 cut(s) 255, 261, 371
SaqAI TTAA 2 cut(s) 98, 758
SatI GCNGC 6 cut(s) 6, 39, 42, 76, 197, 842
Sau3AI GATC 6 cut(s) 47, 57, 120, 162, 649, 683
Sau96I GGNCC 4 cut(s) 7, 460, 750, 751
SchI GAGTC 3 cut(s) 241, 763, 873
ScrFI CCNGG 1 cut(s) 11
SduI GDGCHC 1 cut(s) 754
SfaNI GCATC 3 cut(s) 253, 494, 507
SgrAI CRCCGGYG 1 cut(s) 29
SinI GGWCC 1 cut(s) 460
SmiMI CAYNNNNRTG 3 cut(s) 255, 261, 371
Sse9I AATT 4 cut(s) 350, 450, 497, 759
SseBI AGGCCT 1 cut(s) 492
SsiI CCGC 6 cut(s) 5, 27, 39, 199, 636, 823
SspMI CTAG 2 cut(s) 225, 719
StuI AGGCCT 1 cut(s) 492
StyD4I CCNGG 1 cut(s) 9
TaaI ACNGT 2 cut(s) 564, 714
TaiI ACGT 7 cut(s) 113, 159, 182, 292, 406, 591, 598
TaqI TCGA 4 cut(s) 123, 165, 441, 568
TasI AATT 4 cut(s) 350, 450, 497, 759
TatI WGTACW 1 cut(s) 295
TauI GCSGC 2 cut(s) 8, 41
TfiI GAWTC 4 cut(s) 18, 220, 233, 800
Tru1I TTAA 2 cut(s) 98, 758
Tru9I TTAA 2 cut(s) 98, 758
TscAI CASTG 3 cut(s) 378, 519, 569
TseFI GTSAC 1 cut(s) 591
TseI GCWGC 4 cut(s) 41, 75, 196, 841
Tsp45I GTSAC 1 cut(s) 591
TspDTI ATGAA 2 cut(s) 267, 813
TspGWI ACGGA 2 cut(s) 30, 76
TspRI CASTG 3 cut(s) 378, 519, 569
VpaK11BI GGWCC 1 cut(s) 460
VspI ATTAAT 1 cut(s) 758
XbaI TCTAGA 1 cut(s) 718
XceI RCATGY 1 cut(s) 260
XmnI GAANNNNTTC 1 cut(s) 179
XspI CTAG 2 cut(s) 225, 719
Zsp2I ATGCAT 1 cut(s) 522
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.