Rh5BG114200

Hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0009230
Physical Location & Seq
Forward (+)
10689397 .. 10693452
4056 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG114200.1

Sequence Viewer

Length: 966 bp
ATGGCGGCCCAGGACGGAATCTGTACCGCCGGCGACGCCGCAGCCCGATCGGCTCTGATCTTCGTCGGCACCGGCTGCTCCAGCTCCGTCCCCAACTTAATGTGCCTCATCCAGCCCTCCGATCCCCCCTGCCACGTCTGCTCTCAGGCCCTCTCCCTTCCCCCCGACCAAAACCCTAACTACAGGTGCAATACGTCGCTTCTGATCGATTATTGCCGGAGCGACGGCGAACACCGCTACGTGTTGATCGATGTGGGGAAGACGTTCAGGGAGCAAGTGCTGCGGTGGTTCACTCGCTATAAGATTCCTAGAGTTGATTCTATTATTTTGACTCATGAACATGCTGATGCAGTTCTTGGTCTGGATGACATACGTGCTGTACAGCCACATAGTTCCACAAATGACATTGAGCCTACTCCCATCTACCTAACTCAATTTGCAATGGAAAGCATTGCAGTGAAGTTCCCTTACTTGGTTCAGAAAAAACTTAAGGAAGGCCAAGAAGTGAGACGAGTTGCACAACTCGACTGGAAAATTATTGAGGACCATTGTGAAAAACCTTTTGTTGCATCAGGCCTACAATTTGTTCCTTTGCCAGTGATGCATGGGGAAGATTATGTCTGCTTGGGGTATCTATTTGGTGAACAGTGTCGAGTAGCTTATATATCTGACGTGTCACGTTTCCTTTCAAGCACAGAGCATTTGATTTCAAAAGATGGCGGTGGGCAGTTGGATCTTCTTATCTTGGACACCTTATACAAGAATGGATCGCACAATGTTCACTTGTGCTTGCCACAGTCTCTAGAAGCTGTAAAGAGAATTTGTCCTAAACGGGCCCTATTAATTGGAATGACTCATCAGTTTGACCACCATAAGGACAATGAATCTCTTATGGAATGGTCTACGCGGGAAGGGATACCTGTGCAGCTTGCGCATGATGGTTTGAGAGTCCCCTTAGACCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00440 map00440
Pfam Domains
Protein Families
Lactamase_B_2

Protein Analysis

321

Amino Acids

35.95

Weight (kDa)

5.84

Isoelectric Point (pI)

43.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Anti-Pycsar_Apyc1 PF23023 77 - 266 2.3e-06 Anti-Pycsar protein Apyc1
Lactamase_B_2 PF12706 79 - 283 5.9e-22 Beta-lactamase superfamily domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 933
AccB1I GGYRCC 1 cut(s) 68
AccI GTMKAC 1 cut(s) 902
AccII CGCG 1 cut(s) 907
AciI CCGC 7 cut(s) 5, 27, 39, 235, 283, 720, 907
AclWI GGATC 3 cut(s) 116, 741, 775
AcsI RAATTY 1 cut(s) 819
AcyI GRCGYC 1 cut(s) 36
AfaI GTAC 2 cut(s) 25, 381
AfiI CCNNNNNNNGG 2 cut(s) 472, 874
AflII CTTAAG 1 cut(s) 488
AflIII ACRYGT 2 cut(s) 240, 672
AgsI TTSAA 2 cut(s) 690, 711
AjiI CACGTC 2 cut(s) 136, 673
AjnI CCWGG 1 cut(s) 9
AluBI AGCT 4 cut(s) 84, 659, 809, 928
AluI AGCT 4 cut(s) 84, 659, 809, 928
Alw26I GTCTC 2 cut(s) 502, 804
AlwI GGATC 3 cut(s) 116, 741, 775
AoxI GGCC 5 cut(s) 6, 147, 496, 574, 834
ApaI GGGCCC 1 cut(s) 838
ApeKI GCWGC 4 cut(s) 41, 75, 280, 925
ApoI RAATTY 1 cut(s) 819
AseI ATTAAT 1 cut(s) 842
Asp700I GAANNNNTTC 1 cut(s) 263
AspLEI GCGC 1 cut(s) 934
AspS9I GGNCC 5 cut(s) 7, 148, 544, 834, 835
AsuHPI GGTGA 1 cut(s) 653
AvaII GGWCC 1 cut(s) 544
BaeGI GKGCMC 1 cut(s) 838
BanI GGYRCC 1 cut(s) 68
BanII GRGCYC 1 cut(s) 838
BarI GAAGNNNNNNTAC 2 cut(s) 452, 484
BbsI GAAGAC 1 cut(s) 266
BbvI GCAGC 4 cut(s) 53, 62, 267, 937
BccI CCATC 3 cut(s) 428, 710, 932
BceAI ACGGC 1 cut(s) 241
BcgI CGANNNNNNTGC 2 cut(s) 30, 64
BciT130I CCWGG 1 cut(s) 11
BciVI GTATCC 1 cut(s) 909
BcoDI GTCTC 2 cut(s) 502, 804
BfaI CTAG 2 cut(s) 309, 803
BfmI CTRYAG 1 cut(s) 181
BfrI CTTAAG 1 cut(s) 488
BfuI GTATCC 1 cut(s) 909
BglI GCCNNNNNGGC 1 cut(s) 50
BisI GCNGC 6 cut(s) 6, 39, 42, 76, 281, 926
BlsI GCNGC 6 cut(s) 7, 40, 43, 77, 282, 927
Bme1390I CCNGG 1 cut(s) 11
Bme18I GGWCC 1 cut(s) 544
BmgBI CACGTC 2 cut(s) 136, 673
BmgT120I GGNCC 5 cut(s) 7, 148, 544, 834, 835
BmiI GGNNCC 2 cut(s) 70, 836
BmrFI CCNGG 1 cut(s) 11
BmsI GCATC 3 cut(s) 337, 578, 591
BpiI GAAGAC 1 cut(s) 266
BpmI CTGGAG 1 cut(s) 64
Bsa29I ATCGAT 2 cut(s) 207, 249
BsaAI YACGTR 2 cut(s) 241, 374
BsaHI GRCGYC 1 cut(s) 36
BsaJI CCNNGG 1 cut(s) 9
Bsc4I CCNNNNNNNGG 2 cut(s) 472, 874
Bse118I RCCGGY 2 cut(s) 29, 71
Bse1I ACTGG 2 cut(s) 533, 596
Bse3DI GCAATG 2 cut(s) 447, 450
BseBI CCWGG 1 cut(s) 11
BseCI ATCGAT 2 cut(s) 207, 249
BseDI CCNNGG 1 cut(s) 9
BseGI GGATG 2 cut(s) 108, 370
BseLI CCNNNNNNNGG 2 cut(s) 472, 874
BseMI GCAATG 2 cut(s) 447, 450
BseMII CTCAG 1 cut(s) 158
BseNI ACTGG 2 cut(s) 533, 596
BseSI GKGCMC 1 cut(s) 838
BseXI GCAGC 4 cut(s) 53, 62, 267, 937
BsgI GTGCAG 1 cut(s) 944
Bsh1236I CGCG 1 cut(s) 907
Bsh1285I CGRYCG 1 cut(s) 50
BshFI GGCC 5 cut(s) 8, 149, 498, 576, 836
BshNI GGYRCC 1 cut(s) 68
BshVI ATCGAT 2 cut(s) 207, 249
BsiEI CGRYCG 1 cut(s) 50
BsiSI CCGG 3 cut(s) 30, 72, 217
BslFI GGGAC 2 cut(s) 74, 935
BslI CCNNNNNNNGG 2 cut(s) 472, 874
BsmAI GTCTC 2 cut(s) 502, 804
BsmBI CGTCTC 1 cut(s) 502
BsmFI GGGAC 2 cut(s) 74, 935
BsnI GGCC 5 cut(s) 8, 149, 498, 576, 836
Bsp120I GGGCCC 1 cut(s) 834
Bsp1286I GDGCHC 1 cut(s) 838
Bsp1407I TGTACA 1 cut(s) 379
Bsp143I GATC 7 cut(s) 47, 57, 121, 204, 246, 733, 767
BspACI CCGC 7 cut(s) 5, 27, 39, 235, 283, 720, 907
BspANI GGCC 5 cut(s) 8, 149, 498, 576, 836
BspCNI CTCAG 1 cut(s) 157
BspDI ATCGAT 2 cut(s) 207, 249
BspFNI CGCG 1 cut(s) 907
BspHI TCATGA 1 cut(s) 334
BspLI GGNNCC 2 cut(s) 70, 836
BspPI GGATC 3 cut(s) 116, 741, 775
BspT107I GGYRCC 1 cut(s) 68
BspTI CTTAAG 1 cut(s) 488
BsrDI GCAATG 2 cut(s) 447, 450
BsrFI RCCGGY 2 cut(s) 29, 71
BsrGI TGTACA 1 cut(s) 379
BsrI ACTGG 2 cut(s) 533, 596
BssAI RCCGGY 2 cut(s) 29, 71
BssECI CCNNGG 1 cut(s) 9
BssMI GATC 7 cut(s) 47, 57, 121, 204, 246, 733, 767
BssNI GRCGYC 1 cut(s) 36
Bst2UI CCWGG 1 cut(s) 11
Bst4CI ACNGT 2 cut(s) 648, 798
BstACI GRCGYC 1 cut(s) 36
BstAFI CTTAAG 1 cut(s) 488
BstAPI GCANNNNNTGC 2 cut(s) 75, 280
BstAUI TGTACA 1 cut(s) 379
BstBAI YACGTR 2 cut(s) 241, 374
BstC8I GCNNGC 3 cut(s) 31, 791, 930
BstDEI CTNAG 2 cut(s) 144, 955
BstF5I GGATG 2 cut(s) 108, 370
BstFNI CGCG 1 cut(s) 907
BstHHI GCGC 1 cut(s) 934
BstKTI GATC 7 cut(s) 50, 60, 124, 207, 249, 736, 770
BstMAI GTCTC 2 cut(s) 502, 804
BstMBI GATC 7 cut(s) 47, 57, 121, 204, 246, 733, 767
BstMCI CGRYCG 1 cut(s) 50
BstMWI GCNNNNNNNGC 9 cut(s) 35, 50, 75, 81, 138, 234, 280, 601, 931
BstNI CCWGG 1 cut(s) 11
BstNSI RCATGY 1 cut(s) 344
BstSCI CCNGG 1 cut(s) 9
BstSFI CTRYAG 1 cut(s) 181
BstSLI GKGCMC 1 cut(s) 838
BstUI CGCG 1 cut(s) 907
BstV1I GCAGC 4 cut(s) 53, 62, 267, 937
BstV2I GAAGAC 1 cut(s) 266
BstX2I RGATCY 1 cut(s) 733
BstYI RGATCY 1 cut(s) 733
Bsu15I ATCGAT 2 cut(s) 207, 249
BsuI GTATCC 1 cut(s) 909
BsuRI GGCC 5 cut(s) 8, 149, 498, 576, 836
BsuTUI ATCGAT 2 cut(s) 207, 249
BtrI CACGTC 2 cut(s) 136, 673
BtsCI GGATG 2 cut(s) 108, 370
BtsI GCAGTG 1 cut(s) 462
BtsIMutI CAGTG 3 cut(s) 462, 603, 653
Cac8I GCNNGC 3 cut(s) 31, 791, 930
CciI TCATGA 1 cut(s) 334
CfoI GCGC 1 cut(s) 934
Cfr10I RCCGGY 2 cut(s) 29, 71
Cfr13I GGNCC 5 cut(s) 7, 148, 544, 834, 835
ClaI ATCGAT 2 cut(s) 207, 249
CseI GACGC 1 cut(s) 44
Csp6I GTAC 2 cut(s) 24, 380
CviAII CATG 4 cut(s) 335, 341, 605, 935
CviQI GTAC 2 cut(s) 24, 380
DdeI CTNAG 2 cut(s) 144, 955
DpnI GATC 7 cut(s) 49, 59, 123, 206, 248, 735, 769
DpnII GATC 7 cut(s) 47, 57, 121, 204, 246, 733, 767
Eco147I AGGCCT 1 cut(s) 576
Eco24I GRGCYC 1 cut(s) 838
Eco47I GGWCC 1 cut(s) 544
EcoO109I RGGNCCY 2 cut(s) 148, 835
EcoRII CCWGG 1 cut(s) 9
EcoT22I ATGCAT 1 cut(s) 606
EcoT38I GRGCYC 1 cut(s) 838
Esp3I CGTCTC 1 cut(s) 502
FaeI CATG 4 cut(s) 338, 344, 608, 938
FaqI GGGAC 2 cut(s) 74, 935
FatI CATG 4 cut(s) 334, 340, 604, 934
FauI CCCGC 1 cut(s) 900
FblI GTMKAC 1 cut(s) 902
Fnu4HI GCNGC 6 cut(s) 6, 39, 42, 76, 281, 926
FokI GGATG 2 cut(s) 95, 377
FriOI GRGCYC 1 cut(s) 838
Fsp4HI GCNGC 6 cut(s) 6, 39, 42, 76, 281, 926
FspBI CTAG 2 cut(s) 309, 803
FspI TGCGCA 1 cut(s) 933
GlaI GCGC 1 cut(s) 933
GluI GCNGC 6 cut(s) 6, 39, 42, 76, 281, 926
GsuI CTGGAG 1 cut(s) 64
HaeIII GGCC 5 cut(s) 8, 149, 498, 576, 836
HapII CCGG 3 cut(s) 30, 72, 217
HgaI GACGC 1 cut(s) 44
HhaI GCGC 1 cut(s) 934
Hin1I GRCGYC 1 cut(s) 36
Hin1II CATG 4 cut(s) 338, 344, 608, 938
Hin6I GCGC 1 cut(s) 932
HinP1I GCGC 1 cut(s) 932
HinfI GANTC 7 cut(s) 18, 304, 317, 331, 853, 884, 948
HpaII CCGG 3 cut(s) 30, 72, 217
HphI GGTGA 1 cut(s) 653
Hpy166II GTNNAC 4 cut(s) 291, 644, 781, 903
Hpy188I TCNGA 5 cut(s) 57, 121, 204, 480, 670
Hpy188III TCNNGA 3 cut(s) 335, 362, 803
Hpy8I GTNNAC 4 cut(s) 291, 644, 781, 903
Hpy99I CGWCG 4 cut(s) 38, 68, 199, 227
HpyAV CCTTC 3 cut(s) 167, 488, 905
HpyCH4III ACNGT 2 cut(s) 648, 798
HpyCH4IV ACGT 7 cut(s) 135, 194, 240, 263, 373, 672, 679
HpyCH4V TGCA 8 cut(s) 189, 350, 440, 455, 518, 569, 604, 925
HpyF10VI GCNNNNNNNGC 9 cut(s) 35, 50, 75, 81, 138, 234, 280, 601, 931
HpyF3I CTNAG 2 cut(s) 144, 955
HpySE526I ACGT 7 cut(s) 135, 194, 240, 263, 373, 672, 679
Hsp92I GRCGYC 1 cut(s) 36
Hsp92II CATG 4 cut(s) 338, 344, 608, 938
HspAI GCGC 1 cut(s) 932
KroI GCCGGC 1 cut(s) 29
KroNI GCCGGC 1 cut(s) 31
Kzo9I GATC 7 cut(s) 47, 57, 121, 204, 246, 733, 767
LmnI GCTCC 4 cut(s) 83, 89, 219, 271
Lsp1109I GCAGC 4 cut(s) 53, 62, 267, 937
LweI GCATC 3 cut(s) 337, 578, 591
MaeI CTAG 2 cut(s) 309, 803
MaeII ACGT 7 cut(s) 135, 194, 240, 263, 373, 672, 679
MaeIII GTNAC 1 cut(s) 675
MalI GATC 7 cut(s) 49, 59, 123, 206, 248, 735, 769
MboI GATC 7 cut(s) 47, 57, 121, 204, 246, 733, 767
MboII GAAGA 4 cut(s) 52, 271, 623, 728
MflI RGATCY 1 cut(s) 733
MhlI GDGCHC 1 cut(s) 838
MluCI AATT 5 cut(s) 434, 534, 581, 819, 843
MlyI GAGTC 3 cut(s) 325, 847, 957
MmeI TCCRAC 1 cut(s) 711
MnlI CCTC 4 cut(s) 116, 127, 161, 535
Mph1103I ATGCAT 1 cut(s) 606
MreI CGCCGGCG 1 cut(s) 29
MroNI GCCGGC 1 cut(s) 29
MroXI GAANNNNTTC 1 cut(s) 263
MseI TTAA 3 cut(s) 98, 489, 842
MslI CAYNNNNRTG 3 cut(s) 339, 345, 455
MspCI CTTAAG 1 cut(s) 488
MspI CCGG 3 cut(s) 30, 72, 217
MspR9I CCNGG 1 cut(s) 11
MvaI CCWGG 1 cut(s) 11
MvnI CGCG 1 cut(s) 907
MwoI GCNNNNNNNGC 9 cut(s) 35, 50, 75, 81, 138, 234, 280, 601, 931
NaeI GCCGGC 1 cut(s) 31
NdeII GATC 7 cut(s) 47, 57, 121, 204, 246, 733, 767
NgoMIV GCCGGC 1 cut(s) 29
NlaIII CATG 4 cut(s) 338, 344, 608, 938
NlaIV GGNNCC 2 cut(s) 70, 836
NmuCI GTSAC 1 cut(s) 675
NsbI TGCGCA 1 cut(s) 933
NsiI ATGCAT 1 cut(s) 606
NspI RCATGY 1 cut(s) 344
PagI TCATGA 1 cut(s) 334
PceI AGGCCT 1 cut(s) 576
PcsI WCGNNNNNNNCGW 1 cut(s) 246
PdiI GCCGGC 1 cut(s) 31
PdmI GAANNNNTTC 1 cut(s) 263
PfeI GAWTC 4 cut(s) 18, 304, 317, 884
PkrI GCNGC 6 cut(s) 7, 40, 43, 77, 282, 927
Ple19I CGATCG 1 cut(s) 50
PleI GAGTC 3 cut(s) 325, 847, 956
PpsI GAGTC 3 cut(s) 325, 847, 956
Ppu21I YACGTR 2 cut(s) 241, 374
PshBI ATTAAT 1 cut(s) 842
Psp6I CCWGG 1 cut(s) 9
PspGI CCWGG 1 cut(s) 9
PspN4I GGNNCC 2 cut(s) 70, 836
PspOMI GGGCCC 1 cut(s) 834
PspPI GGNCC 5 cut(s) 7, 148, 544, 834, 835
PsuI RGATCY 1 cut(s) 733
PvuI CGATCG 1 cut(s) 50
RsaI GTAC 2 cut(s) 25, 381
RsaNI GTAC 2 cut(s) 24, 380
RseI CAYNNNNRTG 3 cut(s) 339, 345, 455
SaqAI TTAA 3 cut(s) 98, 489, 842
SatI GCNGC 6 cut(s) 6, 39, 42, 76, 281, 926
Sau3AI GATC 7 cut(s) 47, 57, 121, 204, 246, 733, 767
Sau96I GGNCC 5 cut(s) 7, 148, 544, 834, 835
SchI GAGTC 3 cut(s) 325, 847, 957
ScrFI CCNGG 1 cut(s) 11
SduI GDGCHC 1 cut(s) 838
SfaNI GCATC 3 cut(s) 337, 578, 591
SfcI CTRYAG 1 cut(s) 181
SgrAI CRCCGGYG 1 cut(s) 29
SinI GGWCC 1 cut(s) 544
SmiMI CAYNNNNRTG 3 cut(s) 339, 345, 455
SmlI CTYRAG 1 cut(s) 488
SmoI CTYRAG 1 cut(s) 488
Sse9I AATT 5 cut(s) 434, 534, 581, 819, 843
SseBI AGGCCT 1 cut(s) 576
SsiI CCGC 7 cut(s) 5, 27, 39, 235, 283, 720, 907
SspMI CTAG 2 cut(s) 309, 803
StuI AGGCCT 1 cut(s) 576
StyD4I CCNGG 1 cut(s) 9
TaaI ACNGT 2 cut(s) 648, 798
TaiI ACGT 7 cut(s) 138, 197, 243, 266, 376, 675, 682
TaqI TCGA 4 cut(s) 207, 249, 525, 652
TasI AATT 5 cut(s) 434, 534, 581, 819, 843
TatI WGTACW 1 cut(s) 379
TauI GCSGC 2 cut(s) 8, 41
TfiI GAWTC 4 cut(s) 18, 304, 317, 884
Tru1I TTAA 3 cut(s) 98, 489, 842
Tru9I TTAA 3 cut(s) 98, 489, 842
TscAI CASTG 3 cut(s) 462, 603, 653
TseFI GTSAC 1 cut(s) 675
TseI GCWGC 4 cut(s) 41, 75, 280, 925
Tsp45I GTSAC 1 cut(s) 675
TspDTI ATGAA 2 cut(s) 351, 897
TspGWI ACGGA 2 cut(s) 30, 76
TspRI CASTG 3 cut(s) 462, 603, 653
Vha464I CTTAAG 1 cut(s) 488
VpaK11BI GGWCC 1 cut(s) 544
VspI ATTAAT 1 cut(s) 842
XapI RAATTY 1 cut(s) 819
XbaI TCTAGA 1 cut(s) 802
XceI RCATGY 1 cut(s) 344
XmiI GTMKAC 1 cut(s) 902
XmnI GAANNNNTTC 1 cut(s) 263
XspI CTAG 2 cut(s) 309, 803
Zsp2I ATGCAT 1 cut(s) 606
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.