Rmu_sc0013610.1_g000001

Hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0013610.1
OG0009230
Physical Location & Seq
Reverse (-)
944 .. 4853
3910 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0013610.1_g000001.1.cds

Sequence Viewer

Length: 966 bp
atggcggcccaggacggaatctgtaccgccggcgacgcagcggcccgatcggctctgatcttcgtcggcaccggctgctccagctccgtccccaacttaaggtgccttatccagccctccgatcccccctgccacgtctgctctcaggccctctcccttccccccgaccaaaaccctaactacaggtgcaatacgtcgcttctgatcgattattgccggagcgacggcgaacacaggtacgtgttgatcgatgtggggaagacgttcagggagcaagtgctgcggtggttcactcgctataagattcctagagttgattctattattttgactcatgaacatgctgatgcagttcttggtctggatgacatacgtgctgtacagccacatagttccacaaataacattgagcctactcccatctacctaactcaatttgcaatggaaagcattgcagtgaagttcccttacttggttcagaaaaaacttaaggaaggccaagaagtgagacgagttgcacaactcgactggaaaattattgaggaccattgtgaaaaaccttttgttgcatcaggcctacaatttgttcctttgccagtgatgcatggggaagattacgtctgcttggggtatctatttggtgaacagtctcgagtagcttatatatctgacgtgtcacgttttctttcaagcacagagcatttgatttcaaaagatggcggtgggcagttggatcttcttatcttggacacattatacaagaatggatcgcacaatgttcacttgtgcttgccacagtctctagaagctgtaaagaggatttgtcctaaacgggccctattaattggaatgactcatgagtttgaccaccataaggacaatgaatctcttagggaatggtctaagcgggaagggatacctgtgcagcttgcgcatgatggtttgagagtccccttagacctgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00440 map00440
Pfam Domains
Protein Families
Lactamase_B_2

Protein Analysis

321

Amino Acids

36.01

Weight (kDa)

6.15

Isoelectric Point (pI)

49.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 933
AccB1I GGYRCC 2 cut(s) 68, 102
AciI CCGC 6 cut(s) 5, 27, 41, 283, 720, 907
AclWI GGATC 3 cut(s) 116, 741, 775
AfaI GTAC 3 cut(s) 25, 239, 381
AfiI CCNNNNNNNGG 3 cut(s) 99, 472, 874
AflII CTTAAG 2 cut(s) 97, 488
AflIII ACRYGT 2 cut(s) 240, 672
AgsI TTSAA 2 cut(s) 690, 711
AjiI CACGTC 2 cut(s) 136, 673
AjnI CCWGG 1 cut(s) 9
AluBI AGCT 4 cut(s) 84, 659, 809, 928
AluI AGCT 4 cut(s) 84, 659, 809, 928
Alw26I GTCTC 3 cut(s) 502, 654, 804
AlwI GGATC 3 cut(s) 116, 741, 775
Ama87I CYCGRG 1 cut(s) 651
AoxI GGCC 6 cut(s) 6, 42, 147, 496, 574, 834
ApaI GGGCCC 1 cut(s) 838
ApeKI GCWGC 4 cut(s) 38, 75, 280, 925
AseI ATTAAT 1 cut(s) 842
Asp700I GAANNNNTTC 1 cut(s) 263
AspLEI GCGC 1 cut(s) 934
AspS9I GGNCC 6 cut(s) 7, 43, 148, 544, 834, 835
AsuHPI GGTGA 1 cut(s) 653
AvaI CYCGRG 1 cut(s) 651
AvaII GGWCC 1 cut(s) 544
BaeGI GKGCMC 1 cut(s) 838
BanI GGYRCC 2 cut(s) 68, 102
BanII GRGCYC 1 cut(s) 838
BarI GAAGNNNNNNTAC 2 cut(s) 452, 484
BbsI GAAGAC 1 cut(s) 266
BbvI GCAGC 4 cut(s) 50, 62, 267, 937
BccI CCATC 3 cut(s) 428, 710, 932
BceAI ACGGC 1 cut(s) 241
BciT130I CCWGG 1 cut(s) 11
BciVI GTATCC 1 cut(s) 909
BcoDI GTCTC 3 cut(s) 502, 654, 804
BfaI CTAG 2 cut(s) 309, 803
BfmI CTRYAG 1 cut(s) 181
BfrI CTTAAG 2 cut(s) 97, 488
BfuI GTATCC 1 cut(s) 909
BglI GCCNNNNNGGC 1 cut(s) 50
BisI GCNGC 6 cut(s) 6, 39, 42, 76, 281, 926
BlsI GCNGC 6 cut(s) 7, 40, 43, 77, 282, 927
Bme1390I CCNGG 1 cut(s) 11
Bme18I GGWCC 1 cut(s) 544
BmeT110I CYCGRG 1 cut(s) 651
BmgBI CACGTC 2 cut(s) 136, 673
BmgT120I GGNCC 6 cut(s) 7, 43, 148, 544, 834, 835
BmiI GGNNCC 3 cut(s) 70, 104, 836
BmrFI CCNGG 1 cut(s) 11
BmsI GCATC 3 cut(s) 337, 578, 591
BpiI GAAGAC 1 cut(s) 266
BpmI CTGGAG 1 cut(s) 64
Bsa29I ATCGAT 2 cut(s) 207, 249
BsaAI YACGTR 2 cut(s) 241, 374
BsaJI CCNNGG 1 cut(s) 9
Bsc4I CCNNNNNNNGG 3 cut(s) 99, 472, 874
Bse118I RCCGGY 2 cut(s) 29, 71
Bse1I ACTGG 2 cut(s) 533, 596
Bse3DI GCAATG 2 cut(s) 447, 450
BseBI CCWGG 1 cut(s) 11
BseCI ATCGAT 2 cut(s) 207, 249
BseDI CCNNGG 1 cut(s) 9
BseGI GGATG 1 cut(s) 370
BseLI CCNNNNNNNGG 3 cut(s) 99, 472, 874
BseMI GCAATG 2 cut(s) 447, 450
BseMII CTCAG 1 cut(s) 158
BseNI ACTGG 2 cut(s) 533, 596
BseSI GKGCMC 1 cut(s) 838
BseXI GCAGC 4 cut(s) 50, 62, 267, 937
BsgI GTGCAG 1 cut(s) 944
Bsh1285I CGRYCG 1 cut(s) 50
BshFI GGCC 6 cut(s) 8, 44, 149, 498, 576, 836
BshNI GGYRCC 2 cut(s) 68, 102
BshVI ATCGAT 2 cut(s) 207, 249
BsiEI CGRYCG 1 cut(s) 50
BsiHKCI CYCGRG 1 cut(s) 651
BsiSI CCGG 3 cut(s) 30, 72, 217
BslFI GGGAC 2 cut(s) 74, 935
BslI CCNNNNNNNGG 3 cut(s) 99, 472, 874
BsmAI GTCTC 3 cut(s) 502, 654, 804
BsmBI CGTCTC 1 cut(s) 502
BsmFI GGGAC 2 cut(s) 74, 935
BsnI GGCC 6 cut(s) 8, 44, 149, 498, 576, 836
BsoBI CYCGRG 1 cut(s) 651
Bsp120I GGGCCC 1 cut(s) 834
Bsp1286I GDGCHC 1 cut(s) 838
Bsp1407I TGTACA 1 cut(s) 379
Bsp143I GATC 7 cut(s) 47, 57, 121, 204, 246, 733, 767
BspACI CCGC 6 cut(s) 5, 27, 41, 283, 720, 907
BspANI GGCC 6 cut(s) 8, 44, 149, 498, 576, 836
BspCNI CTCAG 1 cut(s) 157
BspDI ATCGAT 2 cut(s) 207, 249
BspHI TCATGA 2 cut(s) 334, 856
BspLI GGNNCC 3 cut(s) 70, 104, 836
BspPI GGATC 3 cut(s) 116, 741, 775
BspT107I GGYRCC 2 cut(s) 68, 102
BspTI CTTAAG 2 cut(s) 97, 488
BsrDI GCAATG 2 cut(s) 447, 450
BsrFI RCCGGY 2 cut(s) 29, 71
BsrGI TGTACA 1 cut(s) 379
BsrI ACTGG 2 cut(s) 533, 596
BssAI RCCGGY 2 cut(s) 29, 71
BssECI CCNNGG 1 cut(s) 9
BssMI GATC 7 cut(s) 47, 57, 121, 204, 246, 733, 767
Bst2UI CCWGG 1 cut(s) 11
Bst4CI ACNGT 2 cut(s) 648, 798
BstAFI CTTAAG 2 cut(s) 97, 488
BstAPI GCANNNNNTGC 2 cut(s) 75, 280
BstAUI TGTACA 1 cut(s) 379
BstBAI YACGTR 2 cut(s) 241, 374
BstC8I GCNNGC 3 cut(s) 31, 791, 930
BstDEI CTNAG 4 cut(s) 144, 890, 903, 955
BstF5I GGATG 1 cut(s) 370
BstHHI GCGC 1 cut(s) 934
BstKTI GATC 7 cut(s) 50, 60, 124, 207, 249, 736, 770
BstMAI GTCTC 3 cut(s) 502, 654, 804
BstMBI GATC 7 cut(s) 47, 57, 121, 204, 246, 733, 767
BstMCI CGRYCG 1 cut(s) 50
BstMWI GCNNNNNNNGC 8 cut(s) 35, 50, 75, 81, 138, 280, 601, 931
BstNI CCWGG 1 cut(s) 11
BstNSI RCATGY 1 cut(s) 344
BstSCI CCNGG 1 cut(s) 9
BstSFI CTRYAG 1 cut(s) 181
BstSLI GKGCMC 1 cut(s) 838
BstV1I GCAGC 4 cut(s) 50, 62, 267, 937
BstV2I GAAGAC 1 cut(s) 266
BstX2I RGATCY 1 cut(s) 733
BstYI RGATCY 1 cut(s) 733
Bsu15I ATCGAT 2 cut(s) 207, 249
BsuI GTATCC 1 cut(s) 909
BsuRI GGCC 6 cut(s) 8, 44, 149, 498, 576, 836
BsuTUI ATCGAT 2 cut(s) 207, 249
BtrI CACGTC 2 cut(s) 136, 673
BtsCI GGATG 1 cut(s) 370
BtsI GCAGTG 1 cut(s) 462
BtsIMutI CAGTG 2 cut(s) 462, 603
Cac8I GCNNGC 3 cut(s) 31, 791, 930
CciI TCATGA 2 cut(s) 334, 856
CfoI GCGC 1 cut(s) 934
Cfr10I RCCGGY 2 cut(s) 29, 71
Cfr13I GGNCC 6 cut(s) 7, 43, 148, 544, 834, 835
ClaI ATCGAT 2 cut(s) 207, 249
CseI GACGC 1 cut(s) 44
Csp6I GTAC 3 cut(s) 24, 238, 380
CviAII CATG 5 cut(s) 335, 341, 605, 857, 935
CviQI GTAC 3 cut(s) 24, 238, 380
DdeI CTNAG 4 cut(s) 144, 890, 903, 955
DpnI GATC 7 cut(s) 49, 59, 123, 206, 248, 735, 769
DpnII GATC 7 cut(s) 47, 57, 121, 204, 246, 733, 767
Eco147I AGGCCT 1 cut(s) 576
Eco24I GRGCYC 1 cut(s) 838
Eco47I GGWCC 1 cut(s) 544
Eco88I CYCGRG 1 cut(s) 651
EcoO109I RGGNCCY 2 cut(s) 148, 835
EcoRII CCWGG 1 cut(s) 9
EcoT22I ATGCAT 1 cut(s) 606
EcoT38I GRGCYC 1 cut(s) 838
Esp3I CGTCTC 1 cut(s) 502
FaeI CATG 5 cut(s) 338, 344, 608, 860, 938
FaqI GGGAC 2 cut(s) 74, 935
FatI CATG 5 cut(s) 334, 340, 604, 856, 934
FauI CCCGC 1 cut(s) 900
Fnu4HI GCNGC 6 cut(s) 6, 39, 42, 76, 281, 926
FokI GGATG 1 cut(s) 377
FriOI GRGCYC 1 cut(s) 838
Fsp4HI GCNGC 6 cut(s) 6, 39, 42, 76, 281, 926
FspBI CTAG 2 cut(s) 309, 803
FspI TGCGCA 1 cut(s) 933
GlaI GCGC 1 cut(s) 933
GluI GCNGC 6 cut(s) 6, 39, 42, 76, 281, 926
GsuI CTGGAG 1 cut(s) 64
HaeIII GGCC 6 cut(s) 8, 44, 149, 498, 576, 836
HapII CCGG 3 cut(s) 30, 72, 217
HgaI GACGC 1 cut(s) 44
HhaI GCGC 1 cut(s) 934
Hin1II CATG 5 cut(s) 338, 344, 608, 860, 938
Hin6I GCGC 1 cut(s) 932
HinP1I GCGC 1 cut(s) 932
HinfI GANTC 7 cut(s) 18, 304, 317, 331, 853, 884, 948
HpaII CCGG 3 cut(s) 30, 72, 217
HphI GGTGA 1 cut(s) 653
Hpy166II GTNNAC 3 cut(s) 291, 644, 781
Hpy188I TCNGA 5 cut(s) 57, 121, 204, 480, 670
Hpy188III TCNNGA 5 cut(s) 335, 362, 651, 803, 857
Hpy8I GTNNAC 3 cut(s) 291, 644, 781
Hpy99I CGWCG 4 cut(s) 38, 68, 199, 227
HpyAV CCTTC 3 cut(s) 167, 488, 905
HpyCH4III ACNGT 2 cut(s) 648, 798
HpyCH4IV ACGT 8 cut(s) 135, 194, 240, 263, 373, 618, 672, 679
HpyCH4V TGCA 8 cut(s) 189, 350, 440, 455, 518, 569, 604, 925
HpyF10VI GCNNNNNNNGC 8 cut(s) 35, 50, 75, 81, 138, 280, 601, 931
HpyF3I CTNAG 4 cut(s) 144, 890, 903, 955
HpySE526I ACGT 8 cut(s) 135, 194, 240, 263, 373, 618, 672, 679
Hsp92II CATG 5 cut(s) 338, 344, 608, 860, 938
HspAI GCGC 1 cut(s) 932
KroI GCCGGC 1 cut(s) 29
KroNI GCCGGC 1 cut(s) 31
Kzo9I GATC 7 cut(s) 47, 57, 121, 204, 246, 733, 767
LmnI GCTCC 4 cut(s) 83, 89, 219, 271
Lsp1109I GCAGC 4 cut(s) 50, 62, 267, 937
LweI GCATC 3 cut(s) 337, 578, 591
MaeI CTAG 2 cut(s) 309, 803
MaeII ACGT 8 cut(s) 135, 194, 240, 263, 373, 618, 672, 679
MaeIII GTNAC 1 cut(s) 675
MalI GATC 7 cut(s) 49, 59, 123, 206, 248, 735, 769
MboI GATC 7 cut(s) 47, 57, 121, 204, 246, 733, 767
MboII GAAGA 4 cut(s) 52, 271, 623, 728
MflI RGATCY 1 cut(s) 733
MhlI GDGCHC 1 cut(s) 838
MluCI AATT 4 cut(s) 434, 534, 581, 843
MlyI GAGTC 3 cut(s) 325, 847, 957
MmeI TCCRAC 1 cut(s) 711
MnlI CCTC 4 cut(s) 127, 161, 535, 810
Mph1103I ATGCAT 1 cut(s) 606
MreI CGCCGGCG 1 cut(s) 29
MroNI GCCGGC 1 cut(s) 29
MroXI GAANNNNTTC 1 cut(s) 263
MseI TTAA 3 cut(s) 98, 489, 842
MslI CAYNNNNRTG 3 cut(s) 339, 345, 455
MspA1I CMGCKG 1 cut(s) 41
MspCI CTTAAG 2 cut(s) 97, 488
MspI CCGG 3 cut(s) 30, 72, 217
MspR9I CCNGG 1 cut(s) 11
MvaI CCWGG 1 cut(s) 11
MwoI GCNNNNNNNGC 8 cut(s) 35, 50, 75, 81, 138, 280, 601, 931
NaeI GCCGGC 1 cut(s) 31
NdeII GATC 7 cut(s) 47, 57, 121, 204, 246, 733, 767
NgoMIV GCCGGC 1 cut(s) 29
NlaIII CATG 5 cut(s) 338, 344, 608, 860, 938
NlaIV GGNNCC 3 cut(s) 70, 104, 836
NmuCI GTSAC 1 cut(s) 675
NsbI TGCGCA 1 cut(s) 933
NsiI ATGCAT 1 cut(s) 606
NspI RCATGY 1 cut(s) 344
PaeR7I CTCGAG 1 cut(s) 651
PagI TCATGA 2 cut(s) 334, 856
PceI AGGCCT 1 cut(s) 576
PcsI WCGNNNNNNNCGW 1 cut(s) 246
PdiI GCCGGC 1 cut(s) 31
PdmI GAANNNNTTC 1 cut(s) 263
PfeI GAWTC 4 cut(s) 18, 304, 317, 884
PkrI GCNGC 6 cut(s) 7, 40, 43, 77, 282, 927
Ple19I CGATCG 1 cut(s) 50
PleI GAGTC 3 cut(s) 325, 847, 956
PpsI GAGTC 3 cut(s) 325, 847, 956
Ppu21I YACGTR 2 cut(s) 241, 374
PshBI ATTAAT 1 cut(s) 842
Psp6I CCWGG 1 cut(s) 9
PspGI CCWGG 1 cut(s) 9
PspN4I GGNNCC 3 cut(s) 70, 104, 836
PspOMI GGGCCC 1 cut(s) 834
PspPI GGNCC 6 cut(s) 7, 43, 148, 544, 834, 835
PsuI RGATCY 1 cut(s) 733
PvuI CGATCG 1 cut(s) 50
RsaI GTAC 3 cut(s) 25, 239, 381
RsaNI GTAC 3 cut(s) 24, 238, 380
RseI CAYNNNNRTG 3 cut(s) 339, 345, 455
SaqAI TTAA 3 cut(s) 98, 489, 842
SatI GCNGC 6 cut(s) 6, 39, 42, 76, 281, 926
Sau3AI GATC 7 cut(s) 47, 57, 121, 204, 246, 733, 767
Sau96I GGNCC 6 cut(s) 7, 43, 148, 544, 834, 835
SchI GAGTC 3 cut(s) 325, 847, 957
ScrFI CCNGG 1 cut(s) 11
SduI GDGCHC 1 cut(s) 838
SfaNI GCATC 3 cut(s) 337, 578, 591
SfcI CTRYAG 1 cut(s) 181
Sfr274I CTCGAG 1 cut(s) 651
SgrAI CRCCGGYG 1 cut(s) 29
SinI GGWCC 1 cut(s) 544
SlaI CTCGAG 1 cut(s) 651
SmiMI CAYNNNNRTG 3 cut(s) 339, 345, 455
SmlI CTYRAG 3 cut(s) 97, 488, 651
SmoI CTYRAG 3 cut(s) 97, 488, 651
Sse9I AATT 4 cut(s) 434, 534, 581, 843
SseBI AGGCCT 1 cut(s) 576
SsiI CCGC 6 cut(s) 5, 27, 41, 283, 720, 907
SspMI CTAG 2 cut(s) 309, 803
StuI AGGCCT 1 cut(s) 576
StyD4I CCNGG 1 cut(s) 9
TaaI ACNGT 2 cut(s) 648, 798
TaiI ACGT 8 cut(s) 138, 197, 243, 266, 376, 621, 675, 682
TaqI TCGA 4 cut(s) 207, 249, 525, 652
TasI AATT 4 cut(s) 434, 534, 581, 843
TatI WGTACW 1 cut(s) 379
TauI GCSGC 2 cut(s) 8, 44
TfiI GAWTC 4 cut(s) 18, 304, 317, 884
Tru1I TTAA 3 cut(s) 98, 489, 842
Tru9I TTAA 3 cut(s) 98, 489, 842
TscAI CASTG 2 cut(s) 462, 603
TseFI GTSAC 1 cut(s) 675
TseI GCWGC 4 cut(s) 38, 75, 280, 925
Tsp45I GTSAC 1 cut(s) 675
TspDTI ATGAA 2 cut(s) 351, 897
TspGWI ACGGA 2 cut(s) 30, 76
TspRI CASTG 2 cut(s) 462, 603
Vha464I CTTAAG 2 cut(s) 97, 488
VpaK11BI GGWCC 1 cut(s) 544
VspI ATTAAT 1 cut(s) 842
XbaI TCTAGA 1 cut(s) 802
XceI RCATGY 1 cut(s) 344
XhoI CTCGAG 1 cut(s) 651
XmnI GAANNNNTTC 1 cut(s) 263
XspI CTAG 2 cut(s) 309, 803
Zsp2I ATGCAT 1 cut(s) 606
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.