AT1G32050

Probably involved in membrane trafficking

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0007929
Physical Location & Seq
Forward (+)
11528385 .. 11531350
2966 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G32050.1

Sequence Viewer

Length: 795 bp
ATGAATCGCCACCACGATCCCAATCCTTTCGATGAGGACGAAGAAATCGTCAATCCTTTTTCGAAAGGTGGTGGAAGGGTTCCTGCTGCATCTAGGCCAGTTGAATATGGTCAAAGCCTTGATGCTACTGTTGATATTCCATTGGATAATATGAATGACTCTTCACAGAAACAGAGAAAGCTTGCTGACTGGGAAGCTGAGCTCAGGAAGAAAGAAATGGATATAAAGCGAAGAGAGGAAGCTATTGCTAAATTTGGTGTGCAGATAGATGATAAAAACTGGCCACCGTTTTTCCCAATCATACACCATGACATTGCTAAAGAGATACCAGTTCATGCACAAAAGCTGCAGTATCTGGCTTTCGCTAGTTGGTTAGGTATCGTTCTGTGTCTGGTATTCAATGTCATTGCAACGATGGTCTGCTGGATTAAAGGCGGAGGTGTTAAAATCTTTTTCCTGGCCACAATATATGCATTGATCGGATGTCCACTCTCTTATGTACTATGGTACAGGCCACTCTACCGAGCCATGAGGACTGACAGTGCTTTGAAGTTTGGTTGGTTTTTCTTCACCTACTTGATTCACATTGGCTTCTGCATCGTTGCTGCCATCGCCCCTCCAATCTTTTTCCATGGAAAATCATTAACGGGTGTGCTTGCAGCAATTGATGTCATCTCAGACAGTTTATTAGCTGGGATCTTCTACTTTATCGGATTCGGTCTCTTCTGCTTGGAGTCACTGCTGAGTCTATGGGTTCTTCAGAAAATTTACCTCTACTTTAGGGGAAACAAGTGA

Protein Analysis

264

Amino Acids

30.06

Weight (kDa)

7.66

Isoelectric Point (pI)

42.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SCAMP PF04144 92 - 262 1.2e-58 SCAMP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 435
AclWI GGATC 2 cut(s) 11, 704
AcoI YGGCCR 2 cut(s) 281, 459
AcsI RAATTY 2 cut(s) 251, 765
AcuI CTGAAG 1 cut(s) 743
AfaI GTAC 2 cut(s) 501, 509
AgsI TTSAA 3 cut(s) 104, 400, 550
AjnI CCWGG 1 cut(s) 456
AluBI AGCT 6 cut(s) 181, 197, 202, 242, 346, 692
AluI AGCT 6 cut(s) 181, 197, 202, 242, 346, 692
Alw21I GWGCWC 1 cut(s) 204
Alw26I GTCTC 1 cut(s) 725
AlwI GGATC 2 cut(s) 11, 704
AlwNI CAGNNNCTG 1 cut(s) 355
AoxI GGCC 4 cut(s) 95, 281, 459, 512
ApeKI GCWGC 4 cut(s) 86, 346, 605, 659
ApoI RAATTY 2 cut(s) 251, 765
ArsI GACNNNNNNTTYG 2 cut(s) 33, 65
AsuHPI GGTGA 1 cut(s) 562
AsuII TTCGAA 1 cut(s) 62
BalI TGGCCA 2 cut(s) 283, 461
BanII GRGCYC 1 cut(s) 204
Bbv12I GWGCWC 1 cut(s) 204
BbvI GCAGC 4 cut(s) 73, 333, 592, 671
BccI CCATC 2 cut(s) 409, 617
BciT130I CCWGG 1 cut(s) 458
BcoDI GTCTC 1 cut(s) 725
BfaI CTAG 2 cut(s) 93, 366
BfmI CTRYAG 1 cut(s) 347
BisI GCNGC 4 cut(s) 87, 347, 606, 660
BlpI GCTNAGC 1 cut(s) 198
BlsI GCNGC 4 cut(s) 88, 348, 607, 661
Bme1390I CCNGG 1 cut(s) 458
BmiI GGNNCC 1 cut(s) 81
BmrFI CCNGG 1 cut(s) 458
BmrI ACTGGG 1 cut(s) 199
BmsI GCATC 3 cut(s) 98, 112, 606
BmuI ACTGGG 1 cut(s) 199
Bpu10I CCTNAGC 1 cut(s) 203
Bpu1102I GCTNAGC 1 cut(s) 198
Bpu14I TTCGAA 1 cut(s) 62
BsaBI GATNNNNATC 1 cut(s) 21
BsaI GGTCTC 1 cut(s) 725
BsaJI CCNNGG 1 cut(s) 631
Bse1I ACTGG 4 cut(s) 98, 194, 284, 329
Bse3DI GCAATG 2 cut(s) 312, 405
Bse8I GATNNNNATC 1 cut(s) 21
BseBI CCWGG 1 cut(s) 458
BseDI CCNNGG 1 cut(s) 631
BseGI GGATG 1 cut(s) 488
BseJI GATNNNNATC 1 cut(s) 21
BseMI GCAATG 2 cut(s) 312, 405
BseMII CTCAG 4 cut(s) 189, 217, 690, 734
BseNI ACTGG 4 cut(s) 98, 194, 284, 329
BseXI GCAGC 4 cut(s) 73, 333, 592, 671
BseYI CCCAGC 1 cut(s) 692
BsgI GTGCAG 1 cut(s) 281
BshFI GGCC 4 cut(s) 97, 283, 461, 514
BsiHKAI GWGCWC 1 cut(s) 204
BsmAI GTCTC 1 cut(s) 725
BsnI GGCC 4 cut(s) 97, 283, 461, 514
Bso31I GGTCTC 1 cut(s) 725
Bsp119I TTCGAA 1 cut(s) 62
Bsp1286I GDGCHC 1 cut(s) 204
Bsp143I GATC 3 cut(s) 16, 477, 696
Bsp1720I GCTNAGC 1 cut(s) 198
Bsp19I CCATGG 1 cut(s) 631
BspACI CCGC 1 cut(s) 435
BspANI GGCC 4 cut(s) 97, 283, 461, 514
BspCNI CTCAG 4 cut(s) 190, 216, 689, 735
BspLI GGNNCC 1 cut(s) 81
BspMAI CTGCAG 1 cut(s) 351
BspPI GGATC 2 cut(s) 11, 704
BspT104I TTCGAA 1 cut(s) 62
BspTNI GGTCTC 1 cut(s) 725
BsrDI GCAATG 2 cut(s) 312, 405
BsrI ACTGG 4 cut(s) 98, 194, 284, 329
BssECI CCNNGG 1 cut(s) 631
BssMI GATC 3 cut(s) 16, 477, 696
BssT1I CCWWGG 1 cut(s) 631
Bst2UI CCWGG 1 cut(s) 458
Bst4CI ACNGT 4 cut(s) 130, 288, 542, 683
Bst6I CTCTTC 3 cut(s) 166, 226, 728
BstBI TTCGAA 1 cut(s) 62
BstC8I GCNNGC 2 cut(s) 183, 657
BstDEI CTNAG 4 cut(s) 198, 203, 676, 743
BstDSI CCRYGG 1 cut(s) 631
BstF5I GGATG 1 cut(s) 488
BstKTI GATC 3 cut(s) 19, 480, 699
BstMAI GTCTC 1 cut(s) 725
BstMBI GATC 3 cut(s) 16, 477, 696
BstMWI GCNNNNNNNGC 1 cut(s) 611
BstNI CCWGG 1 cut(s) 458
BstSCI CCNGG 1 cut(s) 456
BstSFI CTRYAG 1 cut(s) 347
BstV1I GCAGC 4 cut(s) 73, 333, 592, 671
BstX2I RGATCY 1 cut(s) 696
BstYI RGATCY 1 cut(s) 696
BsuRI GGCC 4 cut(s) 97, 283, 461, 514
BtgI CCRYGG 1 cut(s) 631
BtgZI GCGATG 1 cut(s) 595
BtsCI GGATG 1 cut(s) 488
BtsI GCAGTG 1 cut(s) 737
BtsIMutI CAGTG 2 cut(s) 547, 737
Cac8I GCNNGC 2 cut(s) 183, 657
CaiI CAGNNNCTG 1 cut(s) 355
Csp6I GTAC 2 cut(s) 500, 508
CviAII CATG 4 cut(s) 308, 335, 529, 632
CviQI GTAC 2 cut(s) 500, 508
DdeI CTNAG 4 cut(s) 198, 203, 676, 743
DpnI GATC 3 cut(s) 18, 479, 698
DpnII GATC 3 cut(s) 16, 477, 696
EaeI YGGCCR 2 cut(s) 281, 459
Eam1104I CTCTTC 3 cut(s) 166, 226, 728
EarI CTCTTC 3 cut(s) 166, 226, 728
EciI GGCGGA 1 cut(s) 450
Ecl136II GAGCTC 1 cut(s) 202
Eco130I CCWWGG 1 cut(s) 631
Eco24I GRGCYC 1 cut(s) 204
Eco31I GGTCTC 1 cut(s) 725
Eco53kI GAGCTC 1 cut(s) 202
Eco57I CTGAAG 1 cut(s) 743
EcoICRI GAGCTC 1 cut(s) 202
EcoRII CCWGG 1 cut(s) 456
EcoT14I CCWWGG 1 cut(s) 631
EcoT22I ATGCAT 1 cut(s) 475
EcoT38I GRGCYC 1 cut(s) 204
ErhI CCWWGG 1 cut(s) 631
FaeI CATG 4 cut(s) 311, 338, 532, 635
FatI CATG 4 cut(s) 307, 334, 528, 631
Fnu4HI GCNGC 4 cut(s) 87, 347, 606, 660
FokI GGATG 1 cut(s) 495
FriOI GRGCYC 1 cut(s) 204
Fsp4HI GCNGC 4 cut(s) 87, 347, 606, 660
FspBI CTAG 2 cut(s) 93, 366
GluI GCNGC 4 cut(s) 87, 347, 606, 660
GsaI CCCAGC 1 cut(s) 696
HaeIII GGCC 4 cut(s) 97, 283, 461, 514
Hin1II CATG 4 cut(s) 311, 338, 532, 635
HindIII AAGCTT 1 cut(s) 179
HinfI GANTC 6 cut(s) 4, 158, 580, 714, 734, 745
HphI GGTGA 1 cut(s) 562
Hpy166II GTNNAC 1 cut(s) 488
Hpy188I TCNGA 4 cut(s) 482, 679, 713, 762
Hpy188III TCNNGA 1 cut(s) 205
Hpy8I GTNNAC 1 cut(s) 488
HpyAV CCTTC 1 cut(s) 69
HpyCH4III ACNGT 4 cut(s) 130, 288, 542, 683
HpyCH4V TGCA 8 cut(s) 89, 262, 338, 349, 410, 473, 597, 659
HpyF10VI GCNNNNNNNGC 1 cut(s) 611
HpyF3I CTNAG 4 cut(s) 198, 203, 676, 743
Hsp92II CATG 4 cut(s) 311, 338, 532, 635
Kzo9I GATC 3 cut(s) 16, 477, 696
Lsp1109I GCAGC 4 cut(s) 73, 333, 592, 671
LweI GCATC 3 cut(s) 98, 112, 606
MaeI CTAG 2 cut(s) 93, 366
MaeIII GTNAC 1 cut(s) 735
MalI GATC 3 cut(s) 18, 479, 698
MboI GATC 3 cut(s) 16, 477, 696
MboII GAAGA 8 cut(s) 53, 153, 220, 243, 559, 691, 715, 749
MfeI CAATTG 1 cut(s) 663
MflI RGATCY 1 cut(s) 696
MhlI GDGCHC 1 cut(s) 204
MlsI TGGCCA 2 cut(s) 283, 461
MluCI AATT 3 cut(s) 251, 663, 765
MluNI TGGCCA 2 cut(s) 283, 461
MlyI GAGTC 3 cut(s) 152, 743, 754
MnlI CCTC 6 cut(s) 28, 229, 431, 525, 627, 782
Mox20I TGGCCA 2 cut(s) 283, 461
Mph1103I ATGCAT 1 cut(s) 475
MscI TGGCCA 2 cut(s) 283, 461
MseI TTAA 3 cut(s) 429, 444, 644
Msp20I TGGCCA 2 cut(s) 283, 461
MspR9I CCNGG 1 cut(s) 458
MunI CAATTG 1 cut(s) 663
MvaI CCWGG 1 cut(s) 458
MwoI GCNNNNNNNGC 1 cut(s) 611
NcoI CCATGG 1 cut(s) 631
NdeII GATC 3 cut(s) 16, 477, 696
NlaIII CATG 4 cut(s) 311, 338, 532, 635
NlaIV GGNNCC 1 cut(s) 81
NmuCI GTSAC 1 cut(s) 735
NsiI ATGCAT 1 cut(s) 475
NspV TTCGAA 1 cut(s) 62
PcsI WCGNNNNNNNCGW 2 cut(s) 36, 45
PfeI GAWTC 3 cut(s) 4, 580, 714
PkrI GCNGC 4 cut(s) 88, 348, 607, 661
PleI GAGTC 3 cut(s) 152, 742, 753
PpsI GAGTC 3 cut(s) 152, 742, 753
Psp124BI GAGCTC 1 cut(s) 204
Psp6I CCWGG 1 cut(s) 456
PspFI CCCAGC 1 cut(s) 692
PspGI CCWGG 1 cut(s) 456
PspN4I GGNNCC 1 cut(s) 81
PstI CTGCAG 1 cut(s) 351
PstNI CAGNNNCTG 1 cut(s) 355
PsuI RGATCY 1 cut(s) 696
RsaI GTAC 2 cut(s) 501, 509
RsaNI GTAC 2 cut(s) 500, 508
SacI GAGCTC 1 cut(s) 204
SaqAI TTAA 3 cut(s) 429, 444, 644
SatI GCNGC 4 cut(s) 87, 347, 606, 660
Sau3AI GATC 3 cut(s) 16, 477, 696
SchI GAGTC 3 cut(s) 152, 743, 754
ScrFI CCNGG 1 cut(s) 458
SduI GDGCHC 1 cut(s) 204
SfaNI GCATC 3 cut(s) 98, 112, 606
SfcI CTRYAG 1 cut(s) 347
SfuI TTCGAA 1 cut(s) 62
Sse9I AATT 3 cut(s) 251, 663, 765
SsiI CCGC 1 cut(s) 435
SspMI CTAG 2 cut(s) 93, 366
SstI GAGCTC 1 cut(s) 204
StyD4I CCNGG 1 cut(s) 456
StyI CCWWGG 1 cut(s) 631
TaaI ACNGT 4 cut(s) 130, 288, 542, 683
TaqI TCGA 2 cut(s) 30, 62
TaqII GACCGA 1 cut(s) 707
TasI AATT 3 cut(s) 251, 663, 765
TatI WGTACW 1 cut(s) 499
TfiI GAWTC 3 cut(s) 4, 580, 714
Tru1I TTAA 3 cut(s) 429, 444, 644
Tru9I TTAA 3 cut(s) 429, 444, 644
TscAI CASTG 2 cut(s) 547, 744
TseFI GTSAC 1 cut(s) 735
TseI GCWGC 4 cut(s) 86, 346, 605, 659
Tsp45I GTSAC 1 cut(s) 735
TspDTI ATGAA 3 cut(s) 17, 167, 323
TspRI CASTG 2 cut(s) 547, 744
XapI RAATTY 2 cut(s) 251, 765
XspI CTAG 2 cut(s) 93, 366
Zsp2I ATGCAT 1 cut(s) 475
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.