AT1G33780

Uncharacterized ACR, COG1678

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0013496
Physical Location & Seq
Reverse (-)
12244560 .. 12246231
1672 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G33780.1

Sequence Viewer

Length: 978 bp
ATGGATCTATGGACTCTTCAATTAAAAGACGTCACGATCCGAAACCCTATCTTCTCATCATCGATCCCTGAGAAATCATCGTCGTTTTCTAGGAAACTTTGTGAATTGGAGTTTAGGTTTCTCAATCGTAAGGTTTCTGCTTCTCCGTATCGTTCCTTGGTTGTTCGAGCTACCTCGAAGAAGAGTAACGACGATTCTTCTTCTCCTGGAGATGCTTCTCAGGAAAACAAACCTTCTAACGGCAACAAGTCCGGCGATTCCGCGGCTCCGAAATCGTTCGGTTTAAACACGGATTGGAGAGAGTTCAGAGCGAACTTGTTTATGAAAGAGCAGGAAGAAAAGGCGGAAGCTGAGGGTCATGAATCGGAACCTATAGGTTTGAAATGGGCACATCCGATTCCGTTTCCTGAGACTGGTTGTGTTCTAGTTGCTACAGAGAAGCTAGATGGTTATAGAACATTTGCGAGAACTGTTGTGCTTCTTCTTAGAGCAGGTACTAGACACCCACAAGAAGGGCCGTTTGGTGTTGTTATCAACCGTCCACTTCATAAAAACATCAAGCATATGAAATCAACGAAAACCGAGCTCGCTACTACGTTTTCTGAATGTTCTTTGTACTTTGGTGGTCCTCTTGAAGCAAGCATGTTTTTGTTGAAAACTGGTGATAAAACCAAGATACCTGGGTTTGAAGAAGTCATGCCGGGGCTTAACTTTGGTACTCGAAATAGTTTGGATGAAGCTGCAGTGCTTGTAAAGAAGGGAGTTCTTAAGCCACAGGAGTTTAGATTCTTTGTTGGTTACGCCGGTTGGCAGCTAGATCAGCTCAGAGAAGAGATTGAATCAGATTACTGGCATGTTGCTGCGTGCAGCTCGGATTTAATATGCGGAGCTTCTTCGGAAAACTTGTGGGAGGAGATTTTACAGTTAATGGGCGGTCAATACTCCGAGCTTAGCCGAAAACCTAAGCTGGATATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF179

Protein Analysis

325

Amino Acids

36.39

Weight (kDa)

6.63

Isoelectric Point (pI)

48.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF179 PF02622 153 - 311 2.4e-32 AlgH-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013496)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G33780
fragaria_vesca FvH4_5g33090
malus_domestica MD08G1215000.v1.1 MD15G1398000.v1.1
prunus_persica Prupe.1G546400_v2.0.a1
pyrus_communis pycom08g18590 pycom15g35770
rosa_chinensis RchiOBHm_Chr7g0231261
rosa_laevigata RLG00000001423
rosa_multiflora Rmu_sc0003396.1_g000004
rosa_roxburghii Rroxscaffold_3G00229370
rosa_rugosa Rorug07G0265600 Rorug07G0265600
rosa_samantha Rh7AG419300 Rh7BG394500 Rh7CG439000 Rh7DG412900
rosa_wichuraiana Rw7G034660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 33
Acc36I ACCTGC 1 cut(s) 482
AccII CGCG 1 cut(s) 263
AciI CCGC 5 cut(s) 261, 263, 344, 885, 933
AclWI GGATC 3 cut(s) 12, 31, 58
AcyI GRCGYC 1 cut(s) 30
AfaI GTAC 3 cut(s) 496, 617, 718
AfiI CCNNNNNNNGG 3 cut(s) 239, 413, 512
AflII CTTAAG 1 cut(s) 767
AgsI TTSAA 6 cut(s) 20, 382, 635, 655, 689, 839
AjnI CCWGG 2 cut(s) 205, 679
AjuI GAANNNNNNNTTGG 2 cut(s) 504, 536
Alw21I GWGCWC 1 cut(s) 588
Alw26I GTCTC 1 cut(s) 404
AlwI GGATC 3 cut(s) 12, 31, 58
AoxI GGCC 1 cut(s) 515
ApeKI GCWGC 4 cut(s) 740, 811, 860, 867
Asp700I GAANNNNTTC 1 cut(s) 275
AspS9I GGNCC 2 cut(s) 515, 626
AsuC2I CCSGG 1 cut(s) 702
AsuHPI GGTGA 1 cut(s) 674
AvaII GGWCC 1 cut(s) 626
BaeGI GKGCMC 1 cut(s) 391
BaeI ACNNNNGTAYC 2 cut(s) 668, 701
BanII GRGCYC 1 cut(s) 588
Bbv12I GWGCWC 1 cut(s) 588
BbvCI CCTCAGC 1 cut(s) 351
BbvI GCAGC 4 cut(s) 727, 823, 847, 879
BccI CCATC 1 cut(s) 440
BceAI ACGGC 2 cut(s) 256, 502
BciT130I CCWGG 2 cut(s) 207, 681
BcnI CCSGG 1 cut(s) 702
BcoDI GTCTC 1 cut(s) 404
BfaI CTAG 5 cut(s) 90, 425, 443, 498, 815
BfmI CTRYAG 3 cut(s) 372, 432, 741
BfrI CTTAAG 1 cut(s) 767
BfuAI ACCTGC 1 cut(s) 482
BisI GCNGC 5 cut(s) 264, 741, 812, 861, 868
BlpI GCTNAGC 1 cut(s) 950
BlsI GCNGC 5 cut(s) 265, 742, 813, 862, 869
Bme1390I CCNGG 3 cut(s) 207, 681, 702
Bme18I GGWCC 1 cut(s) 626
BmgT120I GGNCC 2 cut(s) 515, 626
BmiI GGNNCC 2 cut(s) 267, 369
BmrFI CCNGG 3 cut(s) 207, 681, 702
BmsI GCATC 1 cut(s) 202
BpmI CTGGAG 1 cut(s) 228
Bpu10I CCTNAGC 2 cut(s) 351, 963
Bpu1102I GCTNAGC 1 cut(s) 950
BpuMI CCSGG 1 cut(s) 702
Bsa29I ATCGAT 1 cut(s) 62
BsaHI GRCGYC 1 cut(s) 30
BsaJI CCNNGG 4 cut(s) 156, 261, 680, 701
BsaXI ACNNNNNCTCC 2 cut(s) 101, 131
Bsc4I CCNNNNNNNGG 3 cut(s) 239, 413, 512
Bse118I RCCGGY 1 cut(s) 803
Bse1I ACTGG 3 cut(s) 418, 664, 854
BseBI CCWGG 2 cut(s) 207, 681
BseCI ATCGAT 1 cut(s) 62
BseDI CCNNGG 4 cut(s) 156, 261, 680, 701
BseGI GGATG 2 cut(s) 391, 739
BseLI CCNNNNNNNGG 3 cut(s) 239, 413, 512
BseMII CTCAG 5 cut(s) 60, 233, 342, 399, 838
BseNI ACTGG 3 cut(s) 418, 664, 854
BseRI GAGGAG 1 cut(s) 926
BseSI GKGCMC 1 cut(s) 391
BseXI GCAGC 4 cut(s) 727, 823, 847, 879
BsgI GTGCAG 1 cut(s) 886
Bsh1236I CGCG 1 cut(s) 263
BshFI GGCC 1 cut(s) 517
BshVI ATCGAT 1 cut(s) 62
BsiHKAI GWGCWC 1 cut(s) 588
BsiSI CCGG 3 cut(s) 252, 701, 804
BslI CCNNNNNNNGG 3 cut(s) 239, 413, 512
BsmAI GTCTC 1 cut(s) 404
BsnI GGCC 1 cut(s) 517
Bsp1286I GDGCHC 2 cut(s) 391, 588
Bsp143I GATC 4 cut(s) 4, 36, 63, 817
Bsp1720I GCTNAGC 1 cut(s) 950
BspACI CCGC 5 cut(s) 261, 263, 344, 885, 933
BspANI GGCC 1 cut(s) 517
BspCNI CTCAG 5 cut(s) 61, 232, 343, 400, 837
BspDI ATCGAT 1 cut(s) 62
BspFNI CGCG 1 cut(s) 263
BspHI TCATGA 1 cut(s) 358
BspLI GGNNCC 2 cut(s) 267, 369
BspMAI CTGCAG 1 cut(s) 745
BspMI ACCTGC 1 cut(s) 482
BspPI GGATC 3 cut(s) 12, 31, 58
BspTI CTTAAG 1 cut(s) 767
BsrFI RCCGGY 1 cut(s) 803
BsrI ACTGG 3 cut(s) 418, 664, 854
BssAI RCCGGY 1 cut(s) 803
BssECI CCNNGG 4 cut(s) 156, 261, 680, 701
BssMI GATC 4 cut(s) 4, 36, 63, 817
BssNI GRCGYC 1 cut(s) 30
BssT1I CCWWGG 1 cut(s) 156
Bst2UI CCWGG 2 cut(s) 207, 681
Bst4CI ACNGT 3 cut(s) 472, 539, 924
Bst6I CTCTTC 3 cut(s) 21, 176, 825
BstACI GRCGYC 1 cut(s) 30
BstAFI CTTAAG 1 cut(s) 767
BstC8I GCNNGC 3 cut(s) 588, 640, 865
BstDEI CTNAG 8 cut(s) 69, 219, 351, 408, 485, 824, 950, 963
BstDSI CCRYGG 1 cut(s) 261
BstF5I GGATG 2 cut(s) 391, 739
BstFNI CGCG 1 cut(s) 263
BstKTI GATC 4 cut(s) 7, 39, 66, 820
BstMAI GTCTC 1 cut(s) 404
BstMBI GATC 4 cut(s) 4, 36, 63, 817
BstMWI GCNNNNNNNGC 1 cut(s) 820
BstNI CCWGG 2 cut(s) 207, 681
BstNSI RCATGY 2 cut(s) 646, 857
BstSCI CCNGG 3 cut(s) 205, 679, 700
BstSFI CTRYAG 3 cut(s) 372, 432, 741
BstSLI GKGCMC 1 cut(s) 391
BstUI CGCG 1 cut(s) 263
BstV1I GCAGC 4 cut(s) 727, 823, 847, 879
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
Bsu15I ATCGAT 1 cut(s) 62
BsuRI GGCC 1 cut(s) 517
BsuTUI ATCGAT 1 cut(s) 62
BtgI CCRYGG 1 cut(s) 261
BtsCI GGATG 2 cut(s) 391, 739
BtsI GCAGTG 1 cut(s) 750
BtsIMutI CAGTG 1 cut(s) 750
BveI ACCTGC 1 cut(s) 482
Cac8I GCNNGC 3 cut(s) 588, 640, 865
CciI TCATGA 1 cut(s) 358
Cfr10I RCCGGY 1 cut(s) 803
Cfr13I GGNCC 2 cut(s) 515, 626
Cfr42I CCGCGG 1 cut(s) 264
ClaI ATCGAT 1 cut(s) 62
Csp6I GTAC 3 cut(s) 495, 616, 717
CviAII CATG 4 cut(s) 359, 643, 697, 854
CviQI GTAC 3 cut(s) 495, 616, 717
DdeI CTNAG 8 cut(s) 69, 219, 351, 408, 485, 824, 950, 963
DpnI GATC 4 cut(s) 6, 38, 65, 819
DpnII GATC 4 cut(s) 4, 36, 63, 817
DraI TTTAAA 1 cut(s) 285
Eam1104I CTCTTC 3 cut(s) 21, 176, 825
EarI CTCTTC 3 cut(s) 21, 176, 825
EciI GGCGGA 1 cut(s) 359
Ecl136II GAGCTC 1 cut(s) 586
Eco130I CCWWGG 1 cut(s) 156
Eco24I GRGCYC 1 cut(s) 588
Eco32I GATATC 1 cut(s) 973
Eco47I GGWCC 1 cut(s) 626
Eco53kI GAGCTC 1 cut(s) 586
EcoICRI GAGCTC 1 cut(s) 586
EcoRII CCWGG 2 cut(s) 205, 679
EcoRV GATATC 1 cut(s) 973
EcoT14I CCWWGG 1 cut(s) 156
EcoT38I GRGCYC 1 cut(s) 588
ErhI CCWWGG 1 cut(s) 156
FaeI CATG 4 cut(s) 362, 646, 700, 857
FatI CATG 4 cut(s) 358, 642, 696, 853
FauNDI CATATG 1 cut(s) 564
Fnu4HI GCNGC 5 cut(s) 264, 741, 812, 861, 868
FokI GGATG 2 cut(s) 378, 746
FriOI GRGCYC 1 cut(s) 588
Fsp4HI GCNGC 5 cut(s) 264, 741, 812, 861, 868
FspBI CTAG 5 cut(s) 90, 425, 443, 498, 815
GluI GCNGC 5 cut(s) 264, 741, 812, 861, 868
GsuI CTGGAG 1 cut(s) 228
HaeIII GGCC 1 cut(s) 517
HapII CCGG 3 cut(s) 252, 701, 804
Hin1I GRCGYC 1 cut(s) 30
Hin1II CATG 4 cut(s) 362, 646, 700, 857
HinfI GANTC 7 cut(s) 13, 194, 257, 362, 397, 786, 839
HpaII CCGG 3 cut(s) 252, 701, 804
HphI GGTGA 1 cut(s) 674
Hpy166II GTNNAC 1 cut(s) 542
Hpy188III TCNNGA 5 cut(s) 34, 221, 359, 407, 632
Hpy8I GTNNAC 1 cut(s) 542
Hpy99I CGWCG 2 cut(s) 85, 194
HpyAV CCTTC 3 cut(s) 243, 506, 751
HpyCH4III ACNGT 3 cut(s) 472, 539, 924
HpyCH4IV ACGT 2 cut(s) 30, 596
HpyCH4V TGCA 2 cut(s) 743, 867
HpyF10VI GCNNNNNNNGC 1 cut(s) 820
HpyF3I CTNAG 8 cut(s) 69, 219, 351, 408, 485, 824, 950, 963
HpySE526I ACGT 2 cut(s) 30, 596
Hsp92I GRCGYC 1 cut(s) 30
Hsp92II CATG 4 cut(s) 362, 646, 700, 857
KspI CCGCGG 1 cut(s) 264
Kzo9I GATC 4 cut(s) 4, 36, 63, 817
LmnI GCTCC 2 cut(s) 271, 887
Lsp1109I GCAGC 4 cut(s) 727, 823, 847, 879
LweI GCATC 1 cut(s) 202
MaeI CTAG 5 cut(s) 90, 425, 443, 498, 815
MaeII ACGT 2 cut(s) 30, 596
MaeIII GTNAC 3 cut(s) 31, 185, 797
MalI GATC 4 cut(s) 6, 38, 65, 819
MboI GATC 4 cut(s) 4, 36, 63, 817
MflI RGATCY 1 cut(s) 4
MhlI GDGCHC 2 cut(s) 391, 588
MluCI AATT 2 cut(s) 20, 104
MlyI GAGTC 1 cut(s) 7
MnlI CCTC 4 cut(s) 184, 346, 639, 904
MroXI GAANNNNTTC 1 cut(s) 275
MseI TTAA 6 cut(s) 23, 284, 708, 768, 878, 926
MspA1I CMGCKG 1 cut(s) 263
MspCI CTTAAG 1 cut(s) 767
MspI CCGG 3 cut(s) 252, 701, 804
MspR9I CCNGG 3 cut(s) 207, 681, 702
MssI GTTTAAAC 1 cut(s) 285
MvaI CCWGG 2 cut(s) 207, 681
MvnI CGCG 1 cut(s) 263
MwoI GCNNNNNNNGC 1 cut(s) 820
NciI CCSGG 1 cut(s) 702
NdeI CATATG 1 cut(s) 564
NdeII GATC 4 cut(s) 4, 36, 63, 817
NlaIII CATG 4 cut(s) 362, 646, 700, 857
NlaIV GGNNCC 2 cut(s) 267, 369
NmuCI GTSAC 1 cut(s) 31
NspI RCATGY 2 cut(s) 646, 857
PagI TCATGA 1 cut(s) 358
PdmI GAANNNNTTC 1 cut(s) 275
PfeI GAWTC 6 cut(s) 194, 257, 362, 397, 786, 839
PfoI TCCNGGA 1 cut(s) 205
PkrI GCNGC 5 cut(s) 265, 742, 813, 862, 869
PleI GAGTC 1 cut(s) 7
PmeI GTTTAAAC 1 cut(s) 285
PpsI GAGTC 1 cut(s) 7
Psp124BI GAGCTC 1 cut(s) 588
Psp6I CCWGG 2 cut(s) 205, 679
PspGI CCWGG 2 cut(s) 205, 679
PspN4I GGNNCC 2 cut(s) 267, 369
PspPI GGNCC 2 cut(s) 515, 626
PstI CTGCAG 1 cut(s) 745
PsuI RGATCY 1 cut(s) 4
RsaI GTAC 3 cut(s) 496, 617, 718
RsaNI GTAC 3 cut(s) 495, 616, 717
SacI GAGCTC 1 cut(s) 588
SacII CCGCGG 1 cut(s) 264
SaqAI TTAA 6 cut(s) 23, 284, 708, 768, 878, 926
SatI GCNGC 5 cut(s) 264, 741, 812, 861, 868
Sau3AI GATC 4 cut(s) 4, 36, 63, 817
Sau96I GGNCC 2 cut(s) 515, 626
SchI GAGTC 1 cut(s) 7
ScrFI CCNGG 3 cut(s) 207, 681, 702
SduI GDGCHC 2 cut(s) 391, 588
SfaNI GCATC 1 cut(s) 202
SfcI CTRYAG 3 cut(s) 372, 432, 741
Sfr303I CCGCGG 1 cut(s) 264
SgrBI CCGCGG 1 cut(s) 264
SinI GGWCC 1 cut(s) 626
SmlI CTYRAG 1 cut(s) 767
SmoI CTYRAG 1 cut(s) 767
Sse9I AATT 2 cut(s) 20, 104
SsiI CCGC 5 cut(s) 261, 263, 344, 885, 933
SspMI CTAG 5 cut(s) 90, 425, 443, 498, 815
SstI GAGCTC 1 cut(s) 588
StyD4I CCNGG 3 cut(s) 205, 679, 700
StyI CCWWGG 1 cut(s) 156
TaaI ACNGT 3 cut(s) 472, 539, 924
TaiI ACGT 2 cut(s) 33, 599
TaqI TCGA 4 cut(s) 62, 166, 176, 721
TasI AATT 2 cut(s) 20, 104
TatI WGTACW 1 cut(s) 615
TauI GCSGC 1 cut(s) 266
TfiI GAWTC 6 cut(s) 194, 257, 362, 397, 786, 839
Tru1I TTAA 6 cut(s) 23, 284, 708, 768, 878, 926
Tru9I TTAA 6 cut(s) 23, 284, 708, 768, 878, 926
TscAI CASTG 1 cut(s) 750
TseFI GTSAC 1 cut(s) 31
TseI GCWGC 4 cut(s) 740, 811, 860, 867
Tsp45I GTSAC 1 cut(s) 31
TspDTI ATGAA 5 cut(s) 338, 375, 536, 581, 750
TspGWI ACGGA 3 cut(s) 135, 305, 390
TspRI CASTG 1 cut(s) 750
Vha464I CTTAAG 1 cut(s) 767
VpaK11BI GGWCC 1 cut(s) 626
XceI RCATGY 2 cut(s) 646, 857
XmnI GAANNNNTTC 1 cut(s) 275
XspI CTAG 5 cut(s) 90, 425, 443, 498, 815
ZraI GACGTC 1 cut(s) 31
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.