Prupe.1G546400_v2.0.a1

Uncharacterized ACR, COG1678

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0013496
Physical Location & Seq
Reverse (-)
44647210 .. 44652823
5614 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G546400.1

Sequence Viewer

Length: 1089 bp
ATGGATCTGTGCTCCCTGCACGTCAAGAACATCAACAGAAGCCCTTTTATGCATAGGAACTCGTTTACTGAGAAACCCATTTCTTGGAACTTCGGAAATGTGAGGCCTTCTTGGGCTGGTGTTGAAGATGGGAGGGACTTTGAGAGTGGGGTCTTGAATTCGAAGCGCAGGGTTTCCTCTAATGGGTTTCGTTCTTTGGTTGTGAGAGCCATGGGGAAGAAGAACAATAATGGAAATTCATCTAACTCTTCCTCTTCTTCAGGAAATGGTGACCGCTCTATTCCAGAAGGTGATGGTGAAAAAGAAAACGATTCTTCAGAAAACTTCAGAGACACTTCTTCCCAGAAAAATTATCGTATAAACTTGGATTGGAGAGAGTTCAGAGCAAAGCTATATACTGGGTGGCAGGGAGAACAAGCGGAATCTGATGCTCAGAACCAAGGTGGGACTCCCTACCAATCGAAACCTCTTGGCCTGAAGTGGGCTCATCCTATTTCCGTACCTGAGACAGGCTGTGTCCTGGTTGCCACAGAAAAGCTTGATGGAGTTCGCACCTTTGAAAGAACTGTTGTTCTTCTCCTCAGATCAGGAACAAGACACCCAGAAGAGGGGCCATTTGGAGTTGTTATCAACAGGCCACTCCACAAAAGGATCAAACACATGAAGCCCACAAATCTTGATCTTGCAACCACATTTTCTGATTGTTCTTTGCATTTTGGTGGGCCTCTCGAGGCAAGCATGTTTTTGTTAAAAACAGCGGGCAAGACAAAGCTTCCTGGGTTTGAAGAAGTGATCCCTGGCCTTTGTTTTGGTGCTCGTAACAGCCTGGATGAAGCTGCTGGGCTAGTAAAGAAGGGTCTTGTTAAGCCCCAGGATTTCAGATTCTTTGTTGGTTATGCTGGGTGGCAGCTGGATCAGTTGACAGAGGAGATTGAATCGGATTATTGGTACGTGGCTGCTTGCAGCTCAAATTTGATTTACGGGGCTTCATCAGAGTCTTCGTCAGAAAGTTTGTGGGAAGAAATTTTGCAGCTAATGGGTGGCCAGTATTCTGAATTGAGTCGAAAGCCTAAGCAGCAGGATATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF179

Protein Analysis

363

Amino Acids

40.32

Weight (kDa)

7.06

Isoelectric Point (pI)

48.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013496)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G33780
fragaria_vesca FvH4_5g33090
malus_domestica MD08G1215000.v1.1 MD15G1398000.v1.1
prunus_persica Prupe.1G546400_v2.0.a1
pyrus_communis pycom08g18590 pycom15g35770
rosa_chinensis RchiOBHm_Chr7g0231261
rosa_laevigata RLG00000001423
rosa_multiflora Rmu_sc0003396.1_g000004
rosa_roxburghii Rroxscaffold_3G00229370
rosa_rugosa Rorug07G0265600 Rorug07G0265600
rosa_samantha Rh7AG419300 Rh7BG394500 Rh7CG439000 Rh7DG412900
rosa_wichuraiana Rw7G034660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 84
AccBSI CCGCTC 1 cut(s) 276
AciI CCGC 3 cut(s) 274, 419, 758
AclWI GGATC 4 cut(s) 12, 659, 787, 921
AcoI YGGCCR 1 cut(s) 1042
AcsI RAATTY 4 cut(s) 157, 235, 970, 1023
AcuI CTGAAG 4 cut(s) 243, 300, 310, 497
AfaI GTAC 2 cut(s) 501, 950
AfiI CCNNNNNNNGG 6 cut(s) 84, 183, 481, 509, 607, 608
AgsI TTSAA 5 cut(s) 125, 157, 560, 785, 935
AjiI CACGTC 1 cut(s) 22
AjnI CCWGG 5 cut(s) 519, 775, 796, 825, 870
AluBI AGCT 7 cut(s) 391, 538, 772, 836, 910, 966, 1033
AluI AGCT 7 cut(s) 391, 538, 772, 836, 910, 966, 1033
Alw21I GWGCWC 2 cut(s) 14, 817
Alw26I GTCTC 2 cut(s) 324, 500
AlwI GGATC 4 cut(s) 12, 659, 787, 921
Ama87I CYCGRG 1 cut(s) 728
AoxI GGCC 7 cut(s) 104, 472, 611, 635, 722, 799, 1042
ApeKI GCWGC 6 cut(s) 836, 907, 956, 963, 1030, 1075
ApoI RAATTY 4 cut(s) 157, 235, 970, 1023
AspLEI GCGC 1 cut(s) 168
AspS9I GGNCC 2 cut(s) 611, 722
AsuHPI GGTGA 3 cut(s) 281, 302, 308
AsuII TTCGAA 1 cut(s) 161
AvaI CYCGRG 1 cut(s) 728
BalI TGGCCA 1 cut(s) 1044
BanII GRGCYC 1 cut(s) 487
BbsI GAAGAC 1 cut(s) 990
Bbv12I GWGCWC 2 cut(s) 14, 817
BbvI GCAGC 5 cut(s) 823, 919, 943, 975, 1042
BccI CCATC 3 cut(s) 122, 287, 536
BciT130I CCWGG 5 cut(s) 521, 777, 798, 827, 872
BcoDI GTCTC 2 cut(s) 324, 500
BfaI CTAG 1 cut(s) 845
BisI GCNGC 6 cut(s) 837, 908, 957, 964, 1031, 1076
BlsI GCNGC 6 cut(s) 838, 909, 958, 965, 1032, 1077
Bme1390I CCNGG 5 cut(s) 521, 777, 798, 827, 872
BmeT110I CYCGRG 1 cut(s) 728
BmgBI CACGTC 1 cut(s) 22
BmgT120I GGNCC 2 cut(s) 611, 722
BmiI GGNNCC 1 cut(s) 612
BmrFI CCNGG 5 cut(s) 521, 777, 798, 827, 872
BmrI ACTGGG 1 cut(s) 408
BmsI GCATC 1 cut(s) 418
BmuI ACTGGG 1 cut(s) 408
BpiI GAAGAC 1 cut(s) 990
Bpu10I CCTNAGC 1 cut(s) 1071
Bpu14I TTCGAA 1 cut(s) 161
BsaAI YACGTR 1 cut(s) 952
BsaJI CCNNGG 5 cut(s) 210, 439, 776, 796, 870
Bsc4I CCNNNNNNNGG 6 cut(s) 84, 183, 481, 509, 607, 608
Bse1I ACTGG 2 cut(s) 403, 1045
BseBI CCWGG 5 cut(s) 521, 777, 798, 827, 872
BseDI CCNNGG 5 cut(s) 210, 439, 776, 796, 870
BseGI GGATG 2 cut(s) 487, 835
BseLI CCNNNNNNNGG 6 cut(s) 84, 183, 481, 509, 607, 608
BseMII CTCAG 4 cut(s) 60, 446, 495, 595
BseNI ACTGG 2 cut(s) 403, 1045
BseRI GAGGAG 2 cut(s) 569, 941
BseXI GCAGC 5 cut(s) 823, 919, 943, 975, 1042
BseYI CCCAGC 2 cut(s) 839, 899
BshFI GGCC 7 cut(s) 106, 474, 613, 637, 724, 801, 1044
BsiHKAI GWGCWC 2 cut(s) 14, 817
BsiHKCI CYCGRG 1 cut(s) 728
BslFI GGGAC 2 cut(s) 149, 460
BslI CCNNNNNNNGG 6 cut(s) 84, 183, 481, 509, 607, 608
BsmAI GTCTC 2 cut(s) 324, 500
BsmFI GGGAC 2 cut(s) 149, 460
BsnI GGCC 7 cut(s) 106, 474, 613, 637, 724, 801, 1044
BsoBI CYCGRG 1 cut(s) 728
Bsp119I TTCGAA 1 cut(s) 161
Bsp1286I GDGCHC 3 cut(s) 14, 487, 817
Bsp143I GATC 6 cut(s) 4, 584, 651, 679, 792, 913
Bsp19I CCATGG 1 cut(s) 210
BspACI CCGC 3 cut(s) 274, 419, 758
BspANI GGCC 7 cut(s) 106, 474, 613, 637, 724, 801, 1044
BspCNI CTCAG 4 cut(s) 61, 445, 496, 594
BspLI GGNNCC 1 cut(s) 612
BspPI GGATC 4 cut(s) 12, 659, 787, 921
BspT104I TTCGAA 1 cut(s) 161
BsrBI CCGCTC 1 cut(s) 276
BsrI ACTGG 2 cut(s) 403, 1045
BssECI CCNNGG 5 cut(s) 210, 439, 776, 796, 870
BssMI GATC 6 cut(s) 4, 584, 651, 679, 792, 913
BssT1I CCWWGG 2 cut(s) 210, 439
Bst2UI CCWGG 5 cut(s) 521, 777, 798, 827, 872
Bst4CI ACNGT 1 cut(s) 568
Bst6I CTCTTC 3 cut(s) 253, 259, 600
BstBAI YACGTR 1 cut(s) 952
BstBI TTCGAA 1 cut(s) 161
BstC8I GCNNGC 3 cut(s) 736, 760, 961
BstDEI CTNAG 5 cut(s) 69, 432, 504, 581, 1071
BstDSI CCRYGG 1 cut(s) 210
BstEII GGTNACC 1 cut(s) 269
BstENI CCTNNNNNAGG 1 cut(s) 507
BstF5I GGATG 2 cut(s) 487, 835
BstHHI GCGC 1 cut(s) 168
BstKTI GATC 6 cut(s) 7, 587, 654, 682, 795, 916
BstMAI GTCTC 2 cut(s) 324, 500
BstMBI GATC 6 cut(s) 4, 584, 651, 679, 792, 913
BstMWI GCNNNNNNNGC 1 cut(s) 1075
BstNI CCWGG 5 cut(s) 521, 777, 798, 827, 872
BstNSI RCATGY 1 cut(s) 742
BstPI GGTNACC 1 cut(s) 269
BstSCI CCNGG 5 cut(s) 519, 775, 796, 825, 870
BstV1I GCAGC 5 cut(s) 823, 919, 943, 975, 1042
BstV2I GAAGAC 1 cut(s) 990
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
BsuRI GGCC 7 cut(s) 106, 474, 613, 637, 724, 801, 1044
BtgI CCRYGG 1 cut(s) 210
BtrI CACGTC 1 cut(s) 22
BtsCI GGATG 2 cut(s) 487, 835
Cac8I GCNNGC 3 cut(s) 736, 760, 961
CfoI GCGC 1 cut(s) 168
Cfr13I GGNCC 2 cut(s) 611, 722
Csp6I GTAC 2 cut(s) 500, 949
CspCI CAANNNNNGTGG 2 cut(s) 658, 693
CviAII CATG 3 cut(s) 211, 661, 739
CviQI GTAC 2 cut(s) 500, 949
DdeI CTNAG 5 cut(s) 69, 432, 504, 581, 1071
DpnI GATC 6 cut(s) 6, 586, 653, 681, 794, 915
DpnII GATC 6 cut(s) 4, 584, 651, 679, 792, 913
EaeI YGGCCR 1 cut(s) 1042
Eam1104I CTCTTC 3 cut(s) 253, 259, 600
EarI CTCTTC 3 cut(s) 253, 259, 600
Eco130I CCWWGG 2 cut(s) 210, 439
Eco147I AGGCCT 1 cut(s) 106
Eco24I GRGCYC 1 cut(s) 487
Eco57I CTGAAG 4 cut(s) 243, 300, 310, 497
Eco88I CYCGRG 1 cut(s) 728
Eco91I GGTNACC 1 cut(s) 269
EcoNI CCTNNNNNAGG 1 cut(s) 507
EcoO65I GGTNACC 1 cut(s) 269
EcoRI GAATTC 1 cut(s) 157
EcoRII CCWGG 5 cut(s) 519, 775, 796, 825, 870
EcoT14I CCWWGG 2 cut(s) 210, 439
EcoT22I ATGCAT 1 cut(s) 54
EcoT38I GRGCYC 1 cut(s) 487
ErhI CCWWGG 2 cut(s) 210, 439
FaeI CATG 3 cut(s) 214, 664, 742
FaqI GGGAC 2 cut(s) 149, 460
FatI CATG 3 cut(s) 210, 660, 738
FauI CCCGC 1 cut(s) 751
Fnu4HI GCNGC 6 cut(s) 837, 908, 957, 964, 1031, 1076
FokI GGATG 2 cut(s) 474, 842
FriOI GRGCYC 1 cut(s) 487
Fsp4HI GCNGC 6 cut(s) 837, 908, 957, 964, 1031, 1076
FspBI CTAG 1 cut(s) 845
GlaI GCGC 1 cut(s) 167
GluI GCNGC 6 cut(s) 837, 908, 957, 964, 1031, 1076
GsaI CCCAGC 2 cut(s) 843, 903
HaeIII GGCC 7 cut(s) 106, 474, 613, 637, 724, 801, 1044
HhaI GCGC 1 cut(s) 168
Hin1II CATG 3 cut(s) 214, 664, 742
Hin6I GCGC 1 cut(s) 166
HinP1I GCGC 1 cut(s) 166
HincII GTYRAC 1 cut(s) 921
HindII GTYRAC 1 cut(s) 921
HindIII AAGCTT 2 cut(s) 536, 770
HinfI GANTC 7 cut(s) 311, 422, 448, 882, 935, 995, 1060
HphI GGTGA 3 cut(s) 281, 302, 308
Hpy166II GTNNAC 2 cut(s) 66, 921
Hpy188III TCNNGA 7 cut(s) 25, 154, 261, 284, 588, 677, 728
Hpy8I GTNNAC 2 cut(s) 66, 921
HpyAV CCTTC 3 cut(s) 117, 281, 847
HpyCH4III ACNGT 1 cut(s) 568
HpyCH4IV ACGT 2 cut(s) 21, 951
HpyCH4V TGCA 6 cut(s) 19, 52, 686, 712, 963, 1030
HpyF10VI GCNNNNNNNGC 1 cut(s) 1075
HpyF3I CTNAG 5 cut(s) 69, 432, 504, 581, 1071
HpySE526I ACGT 2 cut(s) 21, 951
Hsp92II CATG 3 cut(s) 214, 664, 742
HspAI GCGC 1 cut(s) 166
Kzo9I GATC 6 cut(s) 4, 584, 651, 679, 792, 913
LmnI GCTCC 1 cut(s) 17
Lsp1109I GCAGC 5 cut(s) 823, 919, 943, 975, 1042
LweI GCATC 1 cut(s) 418
MaeI CTAG 1 cut(s) 845
MaeII ACGT 2 cut(s) 21, 951
MaeIII GTNAC 2 cut(s) 269, 818
MalI GATC 6 cut(s) 6, 586, 653, 681, 794, 915
MbiI CCGCTC 1 cut(s) 276
MboI GATC 6 cut(s) 4, 584, 651, 679, 792, 913
MflI RGATCY 1 cut(s) 4
MhlI GDGCHC 3 cut(s) 14, 487, 817
MlsI TGGCCA 1 cut(s) 1044
MluCI AATT 6 cut(s) 157, 235, 349, 970, 1023, 1055
MluNI TGGCCA 1 cut(s) 1044
MlyI GAGTC 3 cut(s) 442, 1004, 1069
Mox20I TGGCCA 1 cut(s) 1044
Mph1103I ATGCAT 1 cut(s) 54
MscI TGGCCA 1 cut(s) 1044
MseI TTAA 2 cut(s) 749, 864
MslI CAYNNNNRTG 1 cut(s) 717
Msp20I TGGCCA 1 cut(s) 1044
MspA1I CMGCKG 2 cut(s) 758, 910
MspR9I CCNGG 5 cut(s) 521, 777, 798, 827, 872
MvaI CCWGG 5 cut(s) 521, 777, 798, 827, 872
MwoI GCNNNNNNNGC 1 cut(s) 1075
NcoI CCATGG 1 cut(s) 210
NdeII GATC 6 cut(s) 4, 584, 651, 679, 792, 913
NlaIII CATG 3 cut(s) 214, 664, 742
NlaIV GGNNCC 1 cut(s) 612
NmuCI GTSAC 1 cut(s) 269
NsiI ATGCAT 1 cut(s) 54
NspI RCATGY 1 cut(s) 742
NspV TTCGAA 1 cut(s) 161
PaeR7I CTCGAG 1 cut(s) 728
PceI AGGCCT 1 cut(s) 106
PfeI GAWTC 4 cut(s) 311, 422, 882, 935
PflMI CCANNNNNTGG 1 cut(s) 84
PkrI GCNGC 6 cut(s) 838, 909, 958, 965, 1032, 1077
PleI GAGTC 3 cut(s) 442, 1003, 1068
PpsI GAGTC 3 cut(s) 442, 1003, 1068
Ppu21I YACGTR 1 cut(s) 952
Psp6I CCWGG 5 cut(s) 519, 775, 796, 825, 870
PspEI GGTNACC 1 cut(s) 269
PspFI CCCAGC 2 cut(s) 839, 899
PspGI CCWGG 5 cut(s) 519, 775, 796, 825, 870
PspN4I GGNNCC 1 cut(s) 612
PspPI GGNCC 2 cut(s) 611, 722
PsuI RGATCY 1 cut(s) 4
PvuII CAGCTG 1 cut(s) 910
RsaI GTAC 2 cut(s) 501, 950
RsaNI GTAC 2 cut(s) 500, 949
RseI CAYNNNNRTG 1 cut(s) 717
SaqAI TTAA 2 cut(s) 749, 864
SatI GCNGC 6 cut(s) 837, 908, 957, 964, 1031, 1076
Sau3AI GATC 6 cut(s) 4, 584, 651, 679, 792, 913
Sau96I GGNCC 2 cut(s) 611, 722
SchI GAGTC 3 cut(s) 442, 1004, 1069
ScrFI CCNGG 5 cut(s) 521, 777, 798, 827, 872
SduI GDGCHC 3 cut(s) 14, 487, 817
SfaNI GCATC 1 cut(s) 418
Sfr274I CTCGAG 1 cut(s) 728
SfuI TTCGAA 1 cut(s) 161
SlaI CTCGAG 1 cut(s) 728
SmiMI CAYNNNNRTG 1 cut(s) 717
SmlI CTYRAG 1 cut(s) 728
SmoI CTYRAG 1 cut(s) 728
Sse9I AATT 6 cut(s) 157, 235, 349, 970, 1023, 1055
SseBI AGGCCT 1 cut(s) 106
SsiI CCGC 3 cut(s) 274, 419, 758
SspMI CTAG 1 cut(s) 845
StuI AGGCCT 1 cut(s) 106
StyD4I CCNGG 5 cut(s) 519, 775, 796, 825, 870
StyI CCWWGG 2 cut(s) 210, 439
TaaI ACNGT 1 cut(s) 568
TaiI ACGT 2 cut(s) 24, 954
TaqI TCGA 4 cut(s) 161, 461, 729, 1063
TasI AATT 6 cut(s) 157, 235, 349, 970, 1023, 1055
TfiI GAWTC 4 cut(s) 311, 422, 882, 935
Tru1I TTAA 2 cut(s) 749, 864
Tru9I TTAA 2 cut(s) 749, 864
TseFI GTSAC 1 cut(s) 269
TseI GCWGC 6 cut(s) 836, 907, 956, 963, 1030, 1075
Tsp45I GTSAC 1 cut(s) 269
TspDTI ATGAA 4 cut(s) 228, 677, 846, 978
TspGWI ACGGA 1 cut(s) 487
Van91I CCANNNNNTGG 1 cut(s) 84
XagI CCTNNNNNAGG 1 cut(s) 507
XapI RAATTY 4 cut(s) 157, 235, 970, 1023
XceI RCATGY 1 cut(s) 742
XhoI CTCGAG 1 cut(s) 728
XspI CTAG 1 cut(s) 845
Zsp2I ATGCAT 1 cut(s) 54
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.