pycom15g35770

Uncharacterized ACR, COG1678

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0013496
Physical Location & Seq
Reverse (-)
35832651 .. 35837816
5166 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g35770.1

Sequence Viewer

Length: 1254 bp
ATGATACTATTCATTTTACTCTTTATTTTATCTTTATCATTAAAACTCAAAGTTTCTCAACTATTTTCATTAGTTTCTTTCAAATTACTCAGTTTTTATTTCATTTACTTCCGTAAGTTTGGCCGCCGGCGCATACACTTGTTCACAGATACGCCTATAAAATACCACACTAAAATCACAAGGCTGTGCAATAAGCATATTCTCTGCTTCACACCGTCGTTGTCCGCTCTCGTCTCATCTCGTTCTGATTGGTCTCTCCGTTTTGTTTGTTTGTTTGTTTTTTATTTTTGTATCCGTTACCGATTTTCTCGCCCTCCACTCTCTCTCCTCTCTCGCACTCTCATCGTTTCCATCGTTGAGAAGAACAGTGAACTCTCTCTCCCGACTCCTATTTTCTTCTCCCCGGAAAACTCCTCTTCCGGCAAGGCCGCCATGGATCTGTGGTCCGTGCGGGCGAATAACATCGGCAGAAGCCCTTTTCTGCTCAGAAACTCGTTTTCTGAGAAACCCATTTCGTTGAGTTTTGTAAATGTGCGGCCTTCCTGGGTTGGCGTTGAAGATGGGAAGGATTTCGACATTGGGGTCTTGAATTTGAAGCGCAGGATTTCGTCTAAAGGGTTTCGTTCTTCAGTTGTCAGAGCCATGGGGAAGAAGAACAATAATGGAAATTCGTCCAACTCTTCGTCTTCGTCAGGAAATGGTGACCGCTCTATTCCAGAAGGTGATAGTGAACAAGAAAACTATTCTTCTGAAAACTTCAGTGACGCTTCTTCCCAGAAAAATTATCAGATAAAGTCGGACTGGAGAGAGGTCAGAGCAAGGCTGTATACTATAGAGCAGGAAGAAAATGCTGAGTCTGATGCTCAGAACCAAGGTGGGGCACCCCACCAGTCGAAACCTCTTGGCCTGAAATGGGCTCATCCTATTTCCGTACCAGAGACAGGCTGTGTCCTTGTTGCCACAGAAAAGCTTGACGGGGTTCGCACCTTTGAAAGAACTGTTGTTCTCCTCCTCAGATCAGGAACCAGACACCCAGAAGAGGGACCATTTGGAGTTGTTATCAACAGGCCACTCCATAAAAAGATTAAACACATGAAGCCCACAAATCCTGATCTTGCAACCACATTTTCTGATTGTTCGTTGCATTTTGGTGGGCCTCTTGAGGCAAGCATGTTTCTGTTAAAAAGCGGGGGCAAGTCAAAGCTTCCTGGGTTTGAAGAGGTCGATCCCTGGCCTTTGTTTTGGTGCTCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF179

Protein Analysis

418

Amino Acids

47.07

Weight (kDa)

9.67

Isoelectric Point (pI)

47.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF179 PF02622 329 - 397 1e-09 AlgH-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013496)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G33780
fragaria_vesca FvH4_5g33090
malus_domestica MD08G1215000.v1.1 MD15G1398000.v1.1
prunus_persica Prupe.1G546400_v2.0.a1
pyrus_communis pycom08g18590 pycom15g35770
rosa_chinensis RchiOBHm_Chr7g0231261
rosa_laevigata RLG00000001423
rosa_multiflora Rmu_sc0003396.1_g000004
rosa_roxburghii Rroxscaffold_3G00229370
rosa_rugosa Rorug07G0265600 Rorug07G0265600
rosa_samantha Rh7AG419300 Rh7BG394500 Rh7CG439000 Rh7DG412900
rosa_wichuraiana Rw7G034660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 581
AccB1I GGYRCC 1 cut(s) 880
AccBSI CCGCTC 2 cut(s) 227, 708
AccI GTMKAC 1 cut(s) 827
AciI CCGC 7 cut(s) 124, 225, 429, 451, 535, 706, 1188
AclWI GGATC 2 cut(s) 444, 1220
AcoI YGGCCR 1 cut(s) 121
AcsI RAATTY 2 cut(s) 589, 667
AcuI CTGAAG 2 cut(s) 612, 742
AfaI GTAC 1 cut(s) 933
AfiI CCNNNNNNNGG 5 cut(s) 877, 913, 941, 1039, 1040
AgsI TTSAA 6 cut(s) 82, 557, 589, 595, 992, 1217
AjnI CCWGG 3 cut(s) 542, 1207, 1229
AloI GAACNNNNNNTCC 2 cut(s) 363, 395
AluBI AGCT 2 cut(s) 970, 1204
AluI AGCT 2 cut(s) 970, 1204
Alw21I GWGCWC 1 cut(s) 1250
Alw26I GTCTC 3 cut(s) 238, 258, 932
AlwI GGATC 2 cut(s) 444, 1220
AoxI GGCC 7 cut(s) 121, 426, 536, 904, 1067, 1154, 1232
ApoI RAATTY 2 cut(s) 589, 667
Asp700I GAANNNNTTC 1 cut(s) 569
AspLEI GCGC 2 cut(s) 132, 600
AspS9I GGNCC 3 cut(s) 444, 1043, 1154
AsuC2I CCSGG 1 cut(s) 404
AsuHPI GGTGA 2 cut(s) 713, 734
AvaII GGWCC 2 cut(s) 444, 1043
BaeGI GKGCMC 1 cut(s) 883
BanI GGYRCC 1 cut(s) 880
BanII GRGCYC 1 cut(s) 919
BbsI GAAGAC 1 cut(s) 678
Bbv12I GWGCWC 1 cut(s) 1250
BccI CCATC 2 cut(s) 359, 554
BcgI CGANNNNNNTGC 2 cut(s) 325, 359
BciT130I CCWGG 3 cut(s) 544, 1209, 1231
BciVI GTATCC 1 cut(s) 302
BcnI CCSGG 1 cut(s) 404
BcoDI GTCTC 3 cut(s) 238, 258, 932
BfmI CTRYAG 1 cut(s) 831
BfuI GTATCC 1 cut(s) 302
BisI GCNGC 3 cut(s) 124, 429, 536
BlsI GCNGC 3 cut(s) 125, 430, 537
Bme1390I CCNGG 4 cut(s) 404, 544, 1209, 1231
Bme18I GGWCC 2 cut(s) 444, 1043
BmgT120I GGNCC 3 cut(s) 444, 1043, 1154
BmiI GGNNCC 3 cut(s) 882, 1024, 1044
BmrFI CCNGG 4 cut(s) 404, 544, 1209, 1231
BmsI GCATC 1 cut(s) 850
BpiI GAAGAC 1 cut(s) 678
BpmI CTGGAG 1 cut(s) 823
BpuEI CTTGAG 1 cut(s) 1181
BpuMI CCSGG 1 cut(s) 404
BsaI GGTCTC 1 cut(s) 258
BsaJI CCNNGG 7 cut(s) 402, 432, 543, 642, 871, 1208, 1229
BsaXI ACNNNNNCTCC 4 cut(s) 309, 339, 363, 393
Bsc4I CCNNNNNNNGG 5 cut(s) 877, 913, 941, 1039, 1040
Bse118I RCCGGY 1 cut(s) 126
Bse1I ACTGG 2 cut(s) 806, 889
BseBI CCWGG 3 cut(s) 544, 1209, 1231
BseDI CCNNGG 7 cut(s) 402, 432, 543, 642, 871, 1208, 1229
BseGI GGATG 1 cut(s) 919
BseLI CCNNNNNNNGG 5 cut(s) 877, 913, 941, 1039, 1040
BseMII CTCAG 6 cut(s) 103, 492, 499, 843, 878, 1027
BseNI ACTGG 2 cut(s) 806, 889
BseRI GAGGAG 4 cut(s) 317, 403, 998, 1001
BseSI GKGCMC 1 cut(s) 883
BshFI GGCC 7 cut(s) 123, 428, 538, 906, 1069, 1156, 1234
BshNI GGYRCC 1 cut(s) 880
BsiHKAI GWGCWC 1 cut(s) 1250
BsiSI CCGG 3 cut(s) 127, 404, 420
BslFI GGGAC 1 cut(s) 1056
BslI CCNNNNNNNGG 5 cut(s) 877, 913, 941, 1039, 1040
BsmAI GTCTC 3 cut(s) 238, 258, 932
BsmBI CGTCTC 1 cut(s) 238
BsmFI GGGAC 1 cut(s) 1056
BsnI GGCC 7 cut(s) 123, 428, 538, 906, 1069, 1156, 1234
Bso31I GGTCTC 1 cut(s) 258
Bsp1286I GDGCHC 3 cut(s) 883, 919, 1250
Bsp143I GATC 4 cut(s) 436, 1016, 1111, 1225
Bsp19I CCATGG 2 cut(s) 432, 642
BspACI CCGC 7 cut(s) 124, 225, 429, 451, 535, 706, 1188
BspANI GGCC 7 cut(s) 123, 428, 538, 906, 1069, 1156, 1234
BspCNI CTCAG 6 cut(s) 102, 493, 498, 844, 877, 1026
BspLI GGNNCC 3 cut(s) 882, 1024, 1044
BspPI GGATC 2 cut(s) 444, 1220
BspT107I GGYRCC 1 cut(s) 880
BspTNI GGTCTC 1 cut(s) 258
BsrBI CCGCTC 2 cut(s) 227, 708
BsrFI RCCGGY 1 cut(s) 126
BsrI ACTGG 2 cut(s) 806, 889
BssAI RCCGGY 1 cut(s) 126
BssECI CCNNGG 7 cut(s) 402, 432, 543, 642, 871, 1208, 1229
BssMI GATC 4 cut(s) 436, 1016, 1111, 1225
BssNAI GTATAC 1 cut(s) 828
BssT1I CCWWGG 3 cut(s) 432, 642, 871
Bst1107I GTATAC 1 cut(s) 828
Bst2UI CCWGG 3 cut(s) 544, 1209, 1231
Bst4CI ACNGT 3 cut(s) 216, 368, 1000
Bst6I CTCTTC 4 cut(s) 421, 685, 1032, 1212
BstC8I GCNNGC 3 cut(s) 128, 453, 1168
BstDEI CTNAG 6 cut(s) 89, 485, 501, 852, 864, 1013
BstDSI CCRYGG 2 cut(s) 432, 642
BstEII GGTNACC 1 cut(s) 701
BstF5I GGATG 1 cut(s) 919
BstHHI GCGC 2 cut(s) 132, 600
BstKTI GATC 4 cut(s) 439, 1019, 1114, 1228
BstMAI GTCTC 3 cut(s) 238, 258, 932
BstMBI GATC 4 cut(s) 436, 1016, 1111, 1225
BstMWI GCNNNNNNNGC 1 cut(s) 129
BstNI CCWGG 3 cut(s) 544, 1209, 1231
BstNSI RCATGY 1 cut(s) 1174
BstPI GGTNACC 1 cut(s) 701
BstSCI CCNGG 4 cut(s) 402, 542, 1207, 1229
BstSFI CTRYAG 1 cut(s) 831
BstSLI GKGCMC 1 cut(s) 883
BstV2I GAAGAC 1 cut(s) 678
BstX2I RGATCY 1 cut(s) 436
BstYI RGATCY 1 cut(s) 436
BstZ17I GTATAC 1 cut(s) 828
BsuI GTATCC 1 cut(s) 302
BsuRI GGCC 7 cut(s) 123, 428, 538, 906, 1069, 1156, 1234
BtgI CCRYGG 2 cut(s) 432, 642
BtsCI GGATG 1 cut(s) 919
BtsIMutI CAGTG 2 cut(s) 373, 766
Cac8I GCNNGC 3 cut(s) 128, 453, 1168
CfoI GCGC 2 cut(s) 132, 600
Cfr10I RCCGGY 1 cut(s) 126
Cfr13I GGNCC 3 cut(s) 444, 1043, 1154
CseI GACGC 1 cut(s) 773
Csp6I GTAC 1 cut(s) 932
CviAII CATG 4 cut(s) 433, 643, 1093, 1171
CviQI GTAC 1 cut(s) 932
DdeI CTNAG 6 cut(s) 89, 485, 501, 852, 864, 1013
DpnI GATC 4 cut(s) 438, 1018, 1113, 1227
DpnII GATC 4 cut(s) 436, 1016, 1111, 1225
DrdI GACNNNNNNGTC 1 cut(s) 581
DseDI GACNNNNNNGTC 1 cut(s) 581
EaeI YGGCCR 1 cut(s) 121
Eam1104I CTCTTC 4 cut(s) 421, 685, 1032, 1212
EarI CTCTTC 4 cut(s) 421, 685, 1032, 1212
Eco130I CCWWGG 3 cut(s) 432, 642, 871
Eco24I GRGCYC 1 cut(s) 919
Eco31I GGTCTC 1 cut(s) 258
Eco47I GGWCC 2 cut(s) 444, 1043
Eco57I CTGAAG 2 cut(s) 612, 742
Eco91I GGTNACC 1 cut(s) 701
EcoO65I GGTNACC 1 cut(s) 701
EcoRII CCWGG 3 cut(s) 542, 1207, 1229
EcoT14I CCWWGG 3 cut(s) 432, 642, 871
EcoT38I GRGCYC 1 cut(s) 919
ErhI CCWWGG 3 cut(s) 432, 642, 871
Esp3I CGTCTC 1 cut(s) 238
FaeI CATG 4 cut(s) 436, 646, 1096, 1174
FaqI GGGAC 1 cut(s) 1056
FatI CATG 4 cut(s) 432, 642, 1092, 1170
FauI CCCGC 2 cut(s) 444, 1181
FblI GTMKAC 1 cut(s) 827
Fnu4HI GCNGC 3 cut(s) 124, 429, 536
FokI GGATG 1 cut(s) 906
FriOI GRGCYC 1 cut(s) 919
Fsp4HI GCNGC 3 cut(s) 124, 429, 536
GlaI GCGC 2 cut(s) 131, 599
GluI GCNGC 3 cut(s) 124, 429, 536
GsuI CTGGAG 1 cut(s) 823
HaeIII GGCC 7 cut(s) 123, 428, 538, 906, 1069, 1156, 1234
HapII CCGG 3 cut(s) 127, 404, 420
HgaI GACGC 1 cut(s) 773
HhaI GCGC 2 cut(s) 132, 600
Hin1II CATG 4 cut(s) 436, 646, 1096, 1174
Hin6I GCGC 2 cut(s) 130, 598
HinP1I GCGC 2 cut(s) 130, 598
HindIII AAGCTT 2 cut(s) 968, 1202
HinfI GANTC 2 cut(s) 385, 854
HpaII CCGG 3 cut(s) 127, 404, 420
HphI GGTGA 2 cut(s) 713, 734
Hpy166II GTNNAC 4 cut(s) 144, 371, 731, 828
Hpy188III TCNNGA 7 cut(s) 382, 586, 693, 716, 1020, 1109, 1160
Hpy8I GTNNAC 4 cut(s) 144, 371, 731, 828
Hpy99I CGWCG 1 cut(s) 220
HpyAV CCTTC 3 cut(s) 549, 559, 713
HpyCH4III ACNGT 3 cut(s) 216, 368, 1000
HpyCH4V TGCA 3 cut(s) 189, 1118, 1144
HpyF10VI GCNNNNNNNGC 1 cut(s) 129
HpyF3I CTNAG 6 cut(s) 89, 485, 501, 852, 864, 1013
Hsp92II CATG 4 cut(s) 436, 646, 1096, 1174
HspAI GCGC 2 cut(s) 130, 598
KroI GCCGGC 1 cut(s) 126
KroNI GCCGGC 1 cut(s) 128
Kzo9I GATC 4 cut(s) 436, 1016, 1111, 1225
LweI GCATC 1 cut(s) 850
MaeIII GTNAC 3 cut(s) 296, 701, 761
MalI GATC 4 cut(s) 438, 1018, 1113, 1227
MbiI CCGCTC 2 cut(s) 227, 708
MboI GATC 4 cut(s) 436, 1016, 1111, 1225
MflI RGATCY 1 cut(s) 436
MhlI GDGCHC 3 cut(s) 883, 919, 1250
MluCI AATT 4 cut(s) 83, 589, 667, 781
MlyI GAGTC 2 cut(s) 379, 863
MmeI TCCRAC 2 cut(s) 699, 777
MreI CGCCGGCG 1 cut(s) 126
MroNI GCCGGC 1 cut(s) 126
MroXI GAANNNNTTC 1 cut(s) 569
MseI TTAA 3 cut(s) 41, 1086, 1181
MslI CAYNNNNRTG 1 cut(s) 1149
MspI CCGG 3 cut(s) 127, 404, 420
MspR9I CCNGG 4 cut(s) 404, 544, 1209, 1231
MvaI CCWGG 3 cut(s) 544, 1209, 1231
MwoI GCNNNNNNNGC 1 cut(s) 129
NaeI GCCGGC 1 cut(s) 128
NciI CCSGG 1 cut(s) 404
NcoI CCATGG 2 cut(s) 432, 642
NdeII GATC 4 cut(s) 436, 1016, 1111, 1225
NgoMIV GCCGGC 1 cut(s) 126
NlaIII CATG 4 cut(s) 436, 646, 1096, 1174
NlaIV GGNNCC 3 cut(s) 882, 1024, 1044
NmuCI GTSAC 2 cut(s) 701, 761
NspI RCATGY 1 cut(s) 1174
PcsI WCGNNNNNNNCGW 1 cut(s) 351
PdiI GCCGGC 1 cut(s) 128
PdmI GAANNNNTTC 1 cut(s) 569
PkrI GCNGC 3 cut(s) 125, 430, 537
PleI GAGTC 2 cut(s) 379, 862
PpsI GAGTC 2 cut(s) 379, 862
Psp6I CCWGG 3 cut(s) 542, 1207, 1229
PspEI GGTNACC 1 cut(s) 701
PspGI CCWGG 3 cut(s) 542, 1207, 1229
PspN4I GGNNCC 3 cut(s) 882, 1024, 1044
PspPI GGNCC 3 cut(s) 444, 1043, 1154
PsuI RGATCY 1 cut(s) 436
RsaI GTAC 1 cut(s) 933
RsaNI GTAC 1 cut(s) 932
RseI CAYNNNNRTG 1 cut(s) 1149
SaqAI TTAA 3 cut(s) 41, 1086, 1181
SatI GCNGC 3 cut(s) 124, 429, 536
Sau3AI GATC 4 cut(s) 436, 1016, 1111, 1225
Sau96I GGNCC 3 cut(s) 444, 1043, 1154
SchI GAGTC 2 cut(s) 379, 863
ScrFI CCNGG 4 cut(s) 404, 544, 1209, 1231
SduI GDGCHC 3 cut(s) 883, 919, 1250
SetI ASST 8 cut(s) 724, 813, 877, 901, 972, 989, 1206, 1224
SfaNI GCATC 1 cut(s) 850
SfcI CTRYAG 1 cut(s) 831
SgrAI CRCCGGYG 1 cut(s) 126
SinI GGWCC 2 cut(s) 444, 1043
SmiMI CAYNNNNRTG 1 cut(s) 1149
SmlI CTYRAG 1 cut(s) 1160
SmoI CTYRAG 1 cut(s) 1160
Sse9I AATT 4 cut(s) 83, 589, 667, 781
SsiI CCGC 7 cut(s) 124, 225, 429, 451, 535, 706, 1188
StyD4I CCNGG 4 cut(s) 402, 542, 1207, 1229
StyI CCWWGG 3 cut(s) 432, 642, 871
TaaI ACNGT 3 cut(s) 216, 368, 1000
TaqI TCGA 3 cut(s) 573, 893, 1224
TasI AATT 4 cut(s) 83, 589, 667, 781
TauI GCSGC 3 cut(s) 126, 431, 538
Tru1I TTAA 3 cut(s) 41, 1086, 1181
Tru9I TTAA 3 cut(s) 41, 1086, 1181
TscAI CASTG 2 cut(s) 373, 766
TseFI GTSAC 2 cut(s) 701, 761
Tsp45I GTSAC 2 cut(s) 701, 761
TspDTI ATGAA 3 cut(s) 57, 91, 1109
TspGWI ACGGA 5 cut(s) 101, 248, 284, 436, 919
TspRI CASTG 2 cut(s) 373, 766
VpaK11BI GGWCC 2 cut(s) 444, 1043
XapI RAATTY 2 cut(s) 589, 667
XceI RCATGY 1 cut(s) 1174
XmiI GTMKAC 1 cut(s) 827
XmnI GAANNNNTTC 1 cut(s) 569
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.