Rroxscaffold_3G00229370

Uncharacterized ACR, COG1678

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0013496
Physical Location & Seq
Reverse (-)
13837191 .. 13842956
5766 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00229370.1

Sequence Viewer

Length: 1050 bp
ATGGATCTGTGGTCCGTTCACGTCAAGAACAACGTCACCAGAGCAGCCCCCTTTCTGCCCAGAAACTCCTTCTCCGAGAAACCCATTTCCTGGAACTGCGGAAAGCCCAGGCCTTTGTGGCTCCGACTTGAAGATGGTGGGAGGGTGTTCGACATTCGCGACTTGAATTTGAAGCCGCGCAGCAGGGACGCTTTGGTGGTCAGAGCCATGGGCAAGAAGAACAACAATCAGGGCAATTCTTCCTCCTCTTCCTCGGGAAATGATGACCACTCTATTCCAGAAGGAGATGGTGCAAAAGAAAACAACTCTGAAAAAAGCAAAGACACTTCTTCCCAGAAAAGTTATCATACAAATTTGGATTGGAGAGAGGTCAGAGCAATGTTTATTCGCAGGGAGCAGGCTGAAGCTGATCCTCAGTACCAGTCGAAACCTCTTGGCGAGAAGTGGGCTCATCCCCTTGCAGTACCTGAGACAGGCTGTATCCTCGTTGCAACAGAAAAGCTTGACGGAGTGCGCACCTTTGAAAGAACTGTTGTTCTCCTCCTCCGATCAGGAAACAGACACCCAGAAGAGGGACCATTTGGTGTTGTTATCAACAGGCCACTACACAAAAAGATTAAACACATGAGACCCACAAATCTTGATCTTGCAACCACATTCTCTGATTGCTCTCTGCATTTTGGTGGGCCTCTTGAGGCAAGCATGTTCTTGTTGAAAACAGGGGGAAGGTCAAAGCTTCCCGGGTTTGAAGAGGTGATCCCTGGTCTTTGTTTTGGTGCTCGTAACAGTCTGGATGAAGCTGCTGGTCTAGTGAAGAAAGGGCTTCTTAAGCCTCAAGATTTCAGATTTTTTGTTGGCTATGCAGGGTGGCAGCTGGACCAGTTGACAGAGGAGATTGAATCCGATTACTGGTATGCGGCTGCTTGCAGCCCTAATTTGATATATGGAGGTTCATCAGATTCTTCGTCAGAGAGTTTGTGGGAGGAAATCTTGCAGCTAATGGGTGGCCAGTATTCTGAATTGAGTCGAAAGCCTAAGCAGGATATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF179

Protein Analysis

349

Amino Acids

39.07

Weight (kDa)

7.66

Isoelectric Point (pI)

48.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF179 PF02622 173 - 335 4.3e-30 AlgH-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013496)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G33780
fragaria_vesca FvH4_5g33090
malus_domestica MD08G1215000.v1.1 MD15G1398000.v1.1
prunus_persica Prupe.1G546400_v2.0.a1
pyrus_communis pycom08g18590 pycom15g35770
rosa_chinensis RchiOBHm_Chr7g0231261
rosa_laevigata RLG00000001423
rosa_multiflora Rmu_sc0003396.1_g000004
rosa_roxburghii Rroxscaffold_3G00229370
rosa_rugosa Rorug07G0265600 Rorug07G0265600
rosa_samantha Rh7AG419300 Rh7BG394500 Rh7CG439000 Rh7DG412900
rosa_wichuraiana Rw7G034660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 515
AccB7I CCANNNNNTGG 1 cut(s) 90
AccII CGCG 2 cut(s) 159, 178
AciI CCGC 3 cut(s) 99, 176, 917
AclWI GGATC 3 cut(s) 12, 404, 751
AcoI YGGCCR 1 cut(s) 1006
AcsI RAATTY 2 cut(s) 166, 352
AcuI CTGAAG 1 cut(s) 423
AfaI GTAC 2 cut(s) 419, 465
AfiI CCNNNNNNNGG 5 cut(s) 90, 473, 571, 572, 909
AflII CTTAAG 1 cut(s) 827
AgsI TTSAA 7 cut(s) 131, 166, 172, 524, 715, 749, 899
AjiI CACGTC 1 cut(s) 22
AjnI CCWGG 3 cut(s) 89, 107, 760
AluBI AGCT 6 cut(s) 407, 502, 736, 800, 874, 997
AluI AGCT 6 cut(s) 407, 502, 736, 800, 874, 997
Alw21I GWGCWC 1 cut(s) 781
Alw26I GTCTC 2 cut(s) 464, 622
AlwI GGATC 3 cut(s) 12, 404, 751
AlwNI CAGNNNCTG 1 cut(s) 467
Ama87I CYCGRG 2 cut(s) 253, 740
AoxI GGCC 4 cut(s) 110, 599, 686, 1006
ApeKI GCWGC 7 cut(s) 44, 180, 800, 871, 920, 927, 994
ApoI RAATTY 2 cut(s) 166, 352
AspLEI GCGC 2 cut(s) 180, 516
AspS9I GGNCC 4 cut(s) 12, 575, 686, 877
AsuC2I CCSGG 2 cut(s) 741, 742
AsuHPI GGTGA 2 cut(s) 28, 766
AvaI CYCGRG 2 cut(s) 253, 740
AvaII GGWCC 3 cut(s) 12, 575, 877
BalI TGGCCA 1 cut(s) 1008
BanII GRGCYC 1 cut(s) 451
Bbv12I GWGCWC 1 cut(s) 781
BbvI GCAGC 7 cut(s) 56, 192, 787, 883, 907, 939, 1006
BccI CCATC 2 cut(s) 128, 281
BciT130I CCWGG 3 cut(s) 91, 109, 762
BciVI GTATCC 1 cut(s) 491
BcnI CCSGG 2 cut(s) 741, 742
BcoDI GTCTC 2 cut(s) 464, 622
BfaI CTAG 1 cut(s) 809
BfrI CTTAAG 1 cut(s) 827
BfuI GTATCC 1 cut(s) 491
BglI GCCNNNNNGGC 1 cut(s) 118
BisI GCNGC 9 cut(s) 45, 176, 181, 801, 872, 918, 921, 928, 995
BlsI GCNGC 9 cut(s) 46, 177, 182, 802, 873, 919, 922, 929, 996
Bme1390I CCNGG 5 cut(s) 91, 109, 741, 742, 762
Bme18I GGWCC 3 cut(s) 12, 575, 877
BmeT110I CYCGRG 2 cut(s) 253, 740
BmgBI CACGTC 1 cut(s) 22
BmgT120I GGNCC 4 cut(s) 12, 575, 686, 877
BmiI GGNNCC 2 cut(s) 122, 576
BmrFI CCNGG 5 cut(s) 91, 109, 741, 742, 762
Bpu10I CCTNAGC 1 cut(s) 1035
BpuEI CTTGAG 2 cut(s) 713, 819
BpuMI CCSGG 2 cut(s) 741, 742
BsaI GGTCTC 1 cut(s) 622
BsaJI CCNNGG 5 cut(s) 107, 207, 252, 740, 760
BsaXI ACNNNNNCTCC 2 cut(s) 56, 86
Bsc4I CCNNNNNNNGG 5 cut(s) 90, 473, 571, 572, 909
Bse1I ACTGG 4 cut(s) 421, 880, 914, 1009
Bse3DI GCAATG 1 cut(s) 384
BseBI CCWGG 3 cut(s) 91, 109, 762
BseDI CCNNGG 5 cut(s) 107, 207, 252, 740, 760
BseGI GGATG 2 cut(s) 451, 799
BseLI CCNNNNNNNGG 5 cut(s) 90, 473, 571, 572, 909
BseMI GCAATG 1 cut(s) 384
BseMII CTCAG 2 cut(s) 428, 459
BseNI ACTGG 4 cut(s) 421, 880, 914, 1009
BseRI GAGGAG 4 cut(s) 235, 530, 533, 905
BseXI GCAGC 7 cut(s) 56, 192, 787, 883, 907, 939, 1006
Bsh1236I CGCG 2 cut(s) 159, 178
BshFI GGCC 4 cut(s) 112, 601, 688, 1008
BsiHKAI GWGCWC 1 cut(s) 781
BsiHKCI CYCGRG 2 cut(s) 253, 740
BsiSI CCGG 1 cut(s) 741
BslFI GGGAC 2 cut(s) 200, 588
BslI CCNNNNNNNGG 5 cut(s) 90, 473, 571, 572, 909
BsmAI GTCTC 2 cut(s) 464, 622
BsmFI GGGAC 2 cut(s) 200, 588
BsnI GGCC 4 cut(s) 112, 601, 688, 1008
Bso31I GGTCTC 1 cut(s) 622
BsoBI CYCGRG 2 cut(s) 253, 740
Bsp1286I GDGCHC 2 cut(s) 451, 781
Bsp143I GATC 5 cut(s) 4, 409, 548, 643, 756
Bsp19I CCATGG 1 cut(s) 207
Bsp68I TCGCGA 1 cut(s) 159
BspACI CCGC 3 cut(s) 99, 176, 917
BspANI GGCC 4 cut(s) 112, 601, 688, 1008
BspCNI CTCAG 2 cut(s) 427, 460
BspFNI CGCG 2 cut(s) 159, 178
BspLI GGNNCC 2 cut(s) 122, 576
BspPI GGATC 3 cut(s) 12, 404, 751
BspTI CTTAAG 1 cut(s) 827
BspTNI GGTCTC 1 cut(s) 622
BsrDI GCAATG 1 cut(s) 384
BsrI ACTGG 4 cut(s) 421, 880, 914, 1009
BssECI CCNNGG 5 cut(s) 107, 207, 252, 740, 760
BssMI GATC 5 cut(s) 4, 409, 548, 643, 756
BssT1I CCWWGG 1 cut(s) 207
Bst2UI CCWGG 3 cut(s) 91, 109, 762
Bst4CI ACNGT 2 cut(s) 532, 788
Bst6I CTCTTC 3 cut(s) 253, 564, 744
BstAFI CTTAAG 1 cut(s) 827
BstC8I GCNNGC 3 cut(s) 399, 700, 925
BstDEI CTNAG 3 cut(s) 414, 468, 1035
BstDSI CCRYGG 1 cut(s) 207
BstENI CCTNNNNNAGG 1 cut(s) 471
BstF5I GGATG 2 cut(s) 451, 799
BstFNI CGCG 2 cut(s) 159, 178
BstHHI GCGC 2 cut(s) 180, 516
BstKTI GATC 5 cut(s) 7, 412, 551, 646, 759
BstMAI GTCTC 2 cut(s) 464, 622
BstMBI GATC 5 cut(s) 4, 409, 548, 643, 756
BstMWI GCNNNNNNNGC 2 cut(s) 118, 829
BstNI CCWGG 3 cut(s) 91, 109, 762
BstNSI RCATGY 1 cut(s) 706
BstSCI CCNGG 5 cut(s) 89, 107, 739, 740, 760
BstUI CGCG 2 cut(s) 159, 178
BstV1I GCAGC 7 cut(s) 56, 192, 787, 883, 907, 939, 1006
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
BsuI GTATCC 1 cut(s) 491
BsuRI GGCC 4 cut(s) 112, 601, 688, 1008
BtgI CCRYGG 1 cut(s) 207
BtrI CACGTC 1 cut(s) 22
BtsCI GGATG 2 cut(s) 451, 799
BtuMI TCGCGA 1 cut(s) 159
Cac8I GCNNGC 3 cut(s) 399, 700, 925
CaiI CAGNNNCTG 1 cut(s) 467
CfoI GCGC 2 cut(s) 180, 516
Cfr13I GGNCC 4 cut(s) 12, 575, 686, 877
Cfr9I CCCGGG 1 cut(s) 740
CseI GACGC 1 cut(s) 197
Csp6I GTAC 2 cut(s) 418, 464
CspCI CAANNNNNGTGG 2 cut(s) 622, 657
CviAII CATG 3 cut(s) 208, 625, 703
CviQI GTAC 2 cut(s) 418, 464
DdeI CTNAG 3 cut(s) 414, 468, 1035
DpnI GATC 5 cut(s) 6, 411, 550, 645, 758
DpnII GATC 5 cut(s) 4, 409, 548, 643, 756
EaeI YGGCCR 1 cut(s) 1006
Eam1104I CTCTTC 3 cut(s) 253, 564, 744
EarI CTCTTC 3 cut(s) 253, 564, 744
Eco130I CCWWGG 1 cut(s) 207
Eco147I AGGCCT 1 cut(s) 112
Eco24I GRGCYC 1 cut(s) 451
Eco31I GGTCTC 1 cut(s) 622
Eco47I GGWCC 3 cut(s) 12, 575, 877
Eco57I CTGAAG 1 cut(s) 423
Eco88I CYCGRG 2 cut(s) 253, 740
EcoNI CCTNNNNNAGG 1 cut(s) 471
EcoRII CCWGG 3 cut(s) 89, 107, 760
EcoT14I CCWWGG 1 cut(s) 207
EcoT38I GRGCYC 1 cut(s) 451
ErhI CCWWGG 1 cut(s) 207
FaeI CATG 3 cut(s) 211, 628, 706
FaiI YATR 9 cut(s) 209, 348, 626, 704, 861, 915, 943, 945, 1046
FalI AAGNNNNNCTT 2 cut(s) 810, 842
FaqI GGGAC 2 cut(s) 200, 588
FatI CATG 3 cut(s) 207, 624, 702
Fnu4HI GCNGC 9 cut(s) 45, 176, 181, 801, 872, 918, 921, 928, 995
FokI GGATG 2 cut(s) 438, 806
FriOI GRGCYC 1 cut(s) 451
Fsp4HI GCNGC 9 cut(s) 45, 176, 181, 801, 872, 918, 921, 928, 995
FspAI RTGCGCAY 1 cut(s) 515
FspBI CTAG 1 cut(s) 809
FspI TGCGCA 1 cut(s) 515
GlaI GCGC 2 cut(s) 179, 515
GluI GCNGC 9 cut(s) 45, 176, 181, 801, 872, 918, 921, 928, 995
HaeIII GGCC 4 cut(s) 112, 601, 688, 1008
HapII CCGG 1 cut(s) 741
HgaI GACGC 1 cut(s) 197
HhaI GCGC 2 cut(s) 180, 516
Hin1II CATG 3 cut(s) 211, 628, 706
Hin6I GCGC 2 cut(s) 178, 514
HinP1I GCGC 2 cut(s) 178, 514
HincII GTYRAC 1 cut(s) 885
HindII GTYRAC 1 cut(s) 885
HindIII AAGCTT 2 cut(s) 500, 734
HinfI GANTC 3 cut(s) 899, 959, 1024
HpaII CCGG 1 cut(s) 741
HphI GGTGA 2 cut(s) 28, 766
Hpy166II GTNNAC 2 cut(s) 19, 885
Hpy188III TCNNGA 9 cut(s) 25, 158, 255, 278, 552, 641, 692, 791, 836
Hpy8I GTNNAC 2 cut(s) 19, 885
HpyAV CCTTC 3 cut(s) 79, 275, 720
HpyCH4III ACNGT 2 cut(s) 532, 788
HpyCH4IV ACGT 2 cut(s) 21, 33
HpyCH4V TGCA 8 cut(s) 293, 461, 491, 650, 676, 863, 927, 994
HpyF10VI GCNNNNNNNGC 2 cut(s) 118, 829
HpyF3I CTNAG 3 cut(s) 414, 468, 1035
HpySE526I ACGT 2 cut(s) 21, 33
Hsp92II CATG 3 cut(s) 211, 628, 706
HspAI GCGC 2 cut(s) 178, 514
Kzo9I GATC 5 cut(s) 4, 409, 548, 643, 756
LmnI GCTCC 2 cut(s) 126, 394
Lsp1109I GCAGC 7 cut(s) 56, 192, 787, 883, 907, 939, 1006
MaeI CTAG 1 cut(s) 809
MaeII ACGT 2 cut(s) 21, 33
MaeIII GTNAC 2 cut(s) 34, 782
MalI GATC 5 cut(s) 6, 411, 550, 645, 758
MboI GATC 5 cut(s) 4, 409, 548, 643, 756
MboII GAAGA 9 cut(s) 143, 229, 231, 240, 321, 581, 761, 826, 954
MflI RGATCY 1 cut(s) 4
MhlI GDGCHC 2 cut(s) 451, 781
MlsI TGGCCA 1 cut(s) 1008
MluCI AATT 5 cut(s) 166, 235, 352, 934, 1019
MluNI TGGCCA 1 cut(s) 1008
MlyI GAGTC 1 cut(s) 1033
MmeI TCCRAC 1 cut(s) 148
Mox20I TGGCCA 1 cut(s) 1008
MscI TGGCCA 1 cut(s) 1008
MseI TTAA 2 cut(s) 618, 828
MslI CAYNNNNRTG 1 cut(s) 681
Msp20I TGGCCA 1 cut(s) 1008
MspA1I CMGCKG 1 cut(s) 874
MspCI CTTAAG 1 cut(s) 827
MspI CCGG 1 cut(s) 741
MspR9I CCNGG 5 cut(s) 91, 109, 741, 742, 762
MvaI CCWGG 3 cut(s) 91, 109, 762
MvnI CGCG 2 cut(s) 159, 178
MwoI GCNNNNNNNGC 2 cut(s) 118, 829
NciI CCSGG 2 cut(s) 741, 742
NcoI CCATGG 1 cut(s) 207
NdeII GATC 5 cut(s) 4, 409, 548, 643, 756
NlaIII CATG 3 cut(s) 211, 628, 706
NlaIV GGNNCC 2 cut(s) 122, 576
NmuCI GTSAC 1 cut(s) 34
NruI TCGCGA 1 cut(s) 159
NsbI TGCGCA 1 cut(s) 515
NspI RCATGY 1 cut(s) 706
PceI AGGCCT 1 cut(s) 112
PcsI WCGNNNNNNNCGW 1 cut(s) 156
PfeI GAWTC 2 cut(s) 899, 959
PflMI CCANNNNNTGG 1 cut(s) 90
PfoI TCCNGGA 1 cut(s) 89
PkrI GCNGC 9 cut(s) 46, 177, 182, 802, 873, 919, 922, 929, 996
PleI GAGTC 1 cut(s) 1032
PpsI GAGTC 1 cut(s) 1032
Psp6I CCWGG 3 cut(s) 89, 107, 760
PspGI CCWGG 3 cut(s) 89, 107, 760
PspN4I GGNNCC 2 cut(s) 122, 576
PspPI GGNCC 4 cut(s) 12, 575, 686, 877
PstNI CAGNNNCTG 1 cut(s) 467
PsuI RGATCY 1 cut(s) 4
PvuII CAGCTG 1 cut(s) 874
RruI TCGCGA 1 cut(s) 159
RsaI GTAC 2 cut(s) 419, 465
RsaNI GTAC 2 cut(s) 418, 464
RseI CAYNNNNRTG 1 cut(s) 681
SaqAI TTAA 2 cut(s) 618, 828
SatI GCNGC 9 cut(s) 45, 176, 181, 801, 872, 918, 921, 928, 995
Sau3AI GATC 5 cut(s) 4, 409, 548, 643, 756
Sau96I GGNCC 4 cut(s) 12, 575, 686, 877
SchI GAGTC 1 cut(s) 1033
ScrFI CCNGG 5 cut(s) 91, 109, 741, 742, 762
SduI GDGCHC 2 cut(s) 451, 781
SinI GGWCC 3 cut(s) 12, 575, 877
SmaI CCCGGG 1 cut(s) 742
SmiMI CAYNNNNRTG 1 cut(s) 681
SmlI CTYRAG 3 cut(s) 692, 827, 834
SmoI CTYRAG 3 cut(s) 692, 827, 834
Sse9I AATT 5 cut(s) 166, 235, 352, 934, 1019
SseBI AGGCCT 1 cut(s) 112
SsiI CCGC 3 cut(s) 99, 176, 917
SspMI CTAG 1 cut(s) 809
StuI AGGCCT 1 cut(s) 112
StyD4I CCNGG 5 cut(s) 89, 107, 739, 740, 760
StyI CCWWGG 1 cut(s) 207
TaaI ACNGT 2 cut(s) 532, 788
TaiI ACGT 2 cut(s) 24, 36
TaqI TCGA 3 cut(s) 150, 425, 1027
TasI AATT 5 cut(s) 166, 235, 352, 934, 1019
TauI GCSGC 2 cut(s) 178, 920
TfiI GAWTC 2 cut(s) 899, 959
Tru1I TTAA 2 cut(s) 618, 828
Tru9I TTAA 2 cut(s) 618, 828
TseFI GTSAC 1 cut(s) 34
TseI GCWGC 7 cut(s) 44, 180, 800, 871, 920, 927, 994
Tsp45I GTSAC 1 cut(s) 34
TspDTI ATGAA 2 cut(s) 810, 942
TspGWI ACGGA 2 cut(s) 4, 522
TspMI CCCGGG 1 cut(s) 740
Van91I CCANNNNNTGG 1 cut(s) 90
Vha464I CTTAAG 1 cut(s) 827
VpaK11BI GGWCC 3 cut(s) 12, 575, 877
XagI CCTNNNNNAGG 1 cut(s) 471
XapI RAATTY 2 cut(s) 166, 352
XceI RCATGY 1 cut(s) 706
XmaI CCCGGG 1 cut(s) 740
XspI CTAG 1 cut(s) 809
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.