MD08G1215000.v1.1

Uncharacterized ACR, COG1678

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0013496
Physical Location & Seq
Reverse (-)
27775506 .. 27784412
8907 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1215000.v1.1.491

Sequence Viewer

Length: 1086 bp
ATGGATCTGTGGTCCGTGCAGGCGAAGAACATCGGCAGAAGCCCTTTTCTACTCAGAAACTCGTTTTCTGATATACCCATTTCGTGGAATTTTGTAAATCTGCGGCCTTCCTGGGTTGGCGTTGAAGACGGGAAGGATTTCGAGATTGGGGTTTTGAATTTGAAGCGCAGGATTTCGTCTAATGGGTTTCGTTCTTCGGCTATCAGAGCCATGGGGAAGAAGAACAATCATGGCAATTCGTCCAACTCTTCGTCTTCGTCAGGAAATGATGACCGATCTATTCCAGAAGGTGATAGTGAACAAGAAAACAATTCTTCTGAAAACTTCAATGACACTTCATCCCAGAAAAATTGTCAGATAAAGTCGGACTGGAGAGAGGTCAGAGCAAGGCTATATACTATGGAGCAGGAAGAAAAGGCTGAGCCTGATGCTCAGAACCAAGGTGGGGAACCCCACCAGTCAAAACCTCTAGGCCTGAAGTGGGCTCATCCTATTTCCGTTCCAGAGACGGGCTGTGTCCTTGTTGCCACAGAAAAGCTTGACGGGGTTCGCACCTTTGAAAGAACTGTCGTTCTCCTCCTCAGATCAGGAAGCAGACATCCAGAAGAGGGACCATTTGGAGTTGTTATCAACAGGCCACTCCATAAAAAGATTAAACACATGAAGCCCACAAATCTTGATCTTGCAACCACATTTTCTGATTGTTCGTTGCATTTTGGTGGGCCTCTTGAGGCAAGCATGTTTTTGTTAAAGAGCGGGGGCAAGTCAAACCTTCCTGGGTTTGAAGAGGTGATCCCTGGGCTTTGTTTTGGTGCTCGTAACAGCCTGGATGAAGCTGCAGCGCTAGTAAAGAAGGGTGTTCTTAAGCCTCAGGATTTCAGATTCTTTGTCGGCTACGCTGGGTGGCAGCTGGATCAGTTGGCAGAGGAGATTGAATCGGATTATTGGTATGTAGCTGCCTGCAGCTCAAATTTGATTTTTGGGGCTTCATCGGAGTCCTCAAAAGAAAGTTTGTGGGAAGAAATTTTGCAGGAAATGGGTGGCCGGTATTCTGAATTGAGTCGAAAGCCGAAGCAGGATATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF179

Protein Analysis

362

Amino Acids

40.17

Weight (kDa)

5.9

Isoelectric Point (pI)

51.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF179 PF02622 185 - 347 2.7e-32 AlgH-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013496)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G33780
fragaria_vesca FvH4_5g33090
malus_domestica MD08G1215000.v1.1 MD15G1398000.v1.1
prunus_persica Prupe.1G546400_v2.0.a1
pyrus_communis pycom08g18590 pycom15g35770
rosa_chinensis RchiOBHm_Chr7g0231261
rosa_laevigata RLG00000001423
rosa_multiflora Rmu_sc0003396.1_g000004
rosa_roxburghii Rroxscaffold_3G00229370
rosa_rugosa Rorug07G0265600 Rorug07G0265600
rosa_samantha Rh7AG419300 Rh7BG394500 Rh7CG439000 Rh7DG412900
rosa_wichuraiana Rw7G034660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 84
AccBSI CCGCTC 1 cut(s) 756
AciI CCGC 2 cut(s) 103, 756
AclWI GGATC 3 cut(s) 12, 787, 921
AcoI YGGCCR 1 cut(s) 1042
AcsI RAATTY 4 cut(s) 88, 157, 970, 1023
AcuI CTGAAG 1 cut(s) 497
AfeI AGCGCT 1 cut(s) 843
AfiI CCNNNNNNNGG 5 cut(s) 84, 445, 481, 509, 608
AflII CTTAAG 1 cut(s) 863
AgsI TTSAA 7 cut(s) 125, 157, 163, 328, 560, 785, 935
AjnI CCWGG 4 cut(s) 110, 775, 796, 825
AluBI AGCT 5 cut(s) 538, 836, 910, 956, 966
AluI AGCT 5 cut(s) 538, 836, 910, 956, 966
Alw21I GWGCWC 1 cut(s) 817
Alw26I GTCTC 1 cut(s) 500
AlwI GGATC 3 cut(s) 12, 787, 921
Aor51HI AGCGCT 1 cut(s) 843
AoxI GGCC 5 cut(s) 104, 472, 635, 722, 1042
ApeKI GCWGC 5 cut(s) 836, 839, 907, 956, 963
ApoI RAATTY 4 cut(s) 88, 157, 970, 1023
Asp700I GAANNNNTTC 1 cut(s) 137
AspLEI GCGC 2 cut(s) 168, 844
AspS9I GGNCC 3 cut(s) 12, 611, 722
AsuHPI GGTGA 2 cut(s) 302, 802
AvaII GGWCC 2 cut(s) 12, 611
AxyI CCTNAGG 1 cut(s) 870
BanII GRGCYC 1 cut(s) 487
BbsI GAAGAC 2 cut(s) 132, 246
Bbv12I GWGCWC 1 cut(s) 817
BbvI GCAGC 5 cut(s) 823, 851, 919, 943, 975
BciT130I CCWGG 4 cut(s) 112, 777, 798, 827
BcoDI GTCTC 1 cut(s) 500
BfaI CTAG 2 cut(s) 470, 845
BfmI CTRYAG 2 cut(s) 837, 961
BfoI RGCGCY 1 cut(s) 845
BfrI CTTAAG 1 cut(s) 863
BisI GCNGC 6 cut(s) 104, 837, 840, 908, 957, 964
BlpI GCTNAGC 1 cut(s) 420
BlsI GCNGC 6 cut(s) 105, 838, 841, 909, 958, 965
Bme1390I CCNGG 4 cut(s) 112, 777, 798, 827
Bme18I GGWCC 2 cut(s) 12, 611
BmgT120I GGNCC 3 cut(s) 12, 611, 722
BmiI GGNNCC 2 cut(s) 450, 612
BmrFI CCNGG 4 cut(s) 112, 777, 798, 827
BmsI GCATC 1 cut(s) 418
BpiI GAAGAC 2 cut(s) 132, 246
BpmI CTGGAG 1 cut(s) 391
Bpu1102I GCTNAGC 1 cut(s) 420
BpuEI CTTGAG 1 cut(s) 749
BsaJI CCNNGG 6 cut(s) 111, 210, 439, 776, 796, 797
Bsc4I CCNNNNNNNGG 5 cut(s) 84, 445, 481, 509, 608
Bse118I RCCGGY 1 cut(s) 1044
Bse1I ACTGG 2 cut(s) 374, 457
Bse21I CCTNAGG 1 cut(s) 870
BseBI CCWGG 4 cut(s) 112, 777, 798, 827
BseDI CCNNGG 6 cut(s) 111, 210, 439, 776, 796, 797
BseGI GGATG 4 cut(s) 338, 487, 598, 835
BseLI CCNNNNNNNGG 5 cut(s) 84, 445, 481, 509, 608
BseMII CTCAG 5 cut(s) 67, 411, 446, 595, 884
BseNI ACTGG 2 cut(s) 374, 457
BseRI GAGGAG 3 cut(s) 566, 569, 941
BseXI GCAGC 5 cut(s) 823, 851, 919, 943, 975
BseYI CCCAGC 1 cut(s) 899
BsgI GTGCAG 1 cut(s) 38
BshFI GGCC 5 cut(s) 106, 474, 637, 724, 1044
BsiHKAI GWGCWC 1 cut(s) 817
BsiSI CCGG 1 cut(s) 1045
BslFI GGGAC 1 cut(s) 624
BslI CCNNNNNNNGG 5 cut(s) 84, 445, 481, 509, 608
BsmAI GTCTC 1 cut(s) 500
BsmBI CGTCTC 1 cut(s) 500
BsmFI GGGAC 1 cut(s) 624
BsnI GGCC 5 cut(s) 106, 474, 637, 724, 1044
Bsp1286I GDGCHC 2 cut(s) 487, 817
Bsp143I GATC 6 cut(s) 4, 275, 584, 679, 792, 913
Bsp1720I GCTNAGC 1 cut(s) 420
Bsp19I CCATGG 1 cut(s) 210
BspACI CCGC 2 cut(s) 103, 756
BspANI GGCC 5 cut(s) 106, 474, 637, 724, 1044
BspCNI CTCAG 5 cut(s) 66, 412, 445, 594, 883
BspLI GGNNCC 2 cut(s) 450, 612
BspMAI CTGCAG 2 cut(s) 841, 965
BspPI GGATC 3 cut(s) 12, 787, 921
BspTI CTTAAG 1 cut(s) 863
BsrBI CCGCTC 1 cut(s) 756
BsrFI RCCGGY 1 cut(s) 1044
BsrI ACTGG 2 cut(s) 374, 457
BssAI RCCGGY 1 cut(s) 1044
BssECI CCNNGG 6 cut(s) 111, 210, 439, 776, 796, 797
BssMI GATC 6 cut(s) 4, 275, 584, 679, 792, 913
BssT1I CCWWGG 2 cut(s) 210, 439
Bst2UI CCWGG 4 cut(s) 112, 777, 798, 827
Bst4CI ACNGT 1 cut(s) 568
Bst6I CTCTTC 3 cut(s) 253, 600, 780
BstAFI CTTAAG 1 cut(s) 863
BstC8I GCNNGC 3 cut(s) 21, 736, 961
BstDEI CTNAG 5 cut(s) 53, 420, 432, 581, 870
BstDSI CCRYGG 1 cut(s) 210
BstF5I GGATG 4 cut(s) 338, 487, 598, 835
BstH2I RGCGCY 1 cut(s) 845
BstHHI GCGC 2 cut(s) 168, 844
BstKTI GATC 6 cut(s) 7, 278, 587, 682, 795, 916
BstMAI GTCTC 1 cut(s) 500
BstMBI GATC 6 cut(s) 4, 275, 584, 679, 792, 913
BstMWI GCNNNNNNNGC 1 cut(s) 206
BstNI CCWGG 4 cut(s) 112, 777, 798, 827
BstNSI RCATGY 1 cut(s) 742
BstSCI CCNGG 4 cut(s) 110, 775, 796, 825
BstSFI CTRYAG 2 cut(s) 837, 961
BstV1I GCAGC 5 cut(s) 823, 851, 919, 943, 975
BstV2I GAAGAC 2 cut(s) 132, 246
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
Bsu36I CCTNAGG 1 cut(s) 870
BsuRI GGCC 5 cut(s) 106, 474, 637, 724, 1044
BtgI CCRYGG 1 cut(s) 210
BtsCI GGATG 4 cut(s) 338, 487, 598, 835
Cac8I GCNNGC 3 cut(s) 21, 736, 961
CfoI GCGC 2 cut(s) 168, 844
Cfr10I RCCGGY 1 cut(s) 1044
Cfr13I GGNCC 3 cut(s) 12, 611, 722
CspCI CAANNNNNGTGG 2 cut(s) 658, 693
CviAII CATG 4 cut(s) 211, 230, 661, 739
DdeI CTNAG 5 cut(s) 53, 420, 432, 581, 870
DpnI GATC 6 cut(s) 6, 277, 586, 681, 794, 915
DpnII GATC 6 cut(s) 4, 275, 584, 679, 792, 913
EaeI YGGCCR 1 cut(s) 1042
Eam1104I CTCTTC 3 cut(s) 253, 600, 780
EarI CTCTTC 3 cut(s) 253, 600, 780
Eco130I CCWWGG 2 cut(s) 210, 439
Eco147I AGGCCT 1 cut(s) 474
Eco24I GRGCYC 1 cut(s) 487
Eco47I GGWCC 2 cut(s) 12, 611
Eco47III AGCGCT 1 cut(s) 843
Eco57I CTGAAG 1 cut(s) 497
Eco81I CCTNAGG 1 cut(s) 870
EcoRII CCWGG 4 cut(s) 110, 775, 796, 825
EcoT14I CCWWGG 2 cut(s) 210, 439
EcoT38I GRGCYC 1 cut(s) 487
ErhI CCWWGG 2 cut(s) 210, 439
Esp3I CGTCTC 1 cut(s) 500
FaeI CATG 4 cut(s) 214, 233, 664, 742
FaqI GGGAC 1 cut(s) 624
FatI CATG 4 cut(s) 210, 229, 660, 738
FauI CCCGC 1 cut(s) 749
Fnu4HI GCNGC 6 cut(s) 104, 837, 840, 908, 957, 964
FokI GGATG 4 cut(s) 325, 474, 585, 842
FriOI GRGCYC 1 cut(s) 487
Fsp4HI GCNGC 6 cut(s) 104, 837, 840, 908, 957, 964
FspBI CTAG 2 cut(s) 470, 845
GlaI GCGC 2 cut(s) 167, 843
GluI GCNGC 6 cut(s) 104, 837, 840, 908, 957, 964
GsaI CCCAGC 1 cut(s) 903
GsuI CTGGAG 1 cut(s) 391
HaeII RGCGCY 1 cut(s) 845
HaeIII GGCC 5 cut(s) 106, 474, 637, 724, 1044
HapII CCGG 1 cut(s) 1045
HhaI GCGC 2 cut(s) 168, 844
Hin1II CATG 4 cut(s) 214, 233, 664, 742
Hin6I GCGC 2 cut(s) 166, 842
HinP1I GCGC 2 cut(s) 166, 842
HindIII AAGCTT 1 cut(s) 536
HinfI GANTC 4 cut(s) 882, 935, 995, 1060
HpaII CCGG 1 cut(s) 1045
HphI GGTGA 2 cut(s) 302, 802
Hpy166II GTNNAC 1 cut(s) 299
Hpy188III TCNNGA 9 cut(s) 142, 261, 284, 503, 588, 602, 677, 728, 872
Hpy8I GTNNAC 1 cut(s) 299
HpyAV CCTTC 5 cut(s) 117, 127, 281, 782, 847
HpyCH4III ACNGT 1 cut(s) 568
HpyCH4V TGCA 6 cut(s) 19, 686, 712, 839, 963, 1030
HpyF10VI GCNNNNNNNGC 1 cut(s) 206
HpyF3I CTNAG 5 cut(s) 53, 420, 432, 581, 870
Hsp92II CATG 4 cut(s) 214, 233, 664, 742
HspAI GCGC 2 cut(s) 166, 842
Kzo9I GATC 6 cut(s) 4, 275, 584, 679, 792, 913
LmnI GCTCC 1 cut(s) 403
Lsp1109I GCAGC 5 cut(s) 823, 851, 919, 943, 975
LweI GCATC 1 cut(s) 418
MaeI CTAG 2 cut(s) 470, 845
MaeIII GTNAC 1 cut(s) 818
MalI GATC 6 cut(s) 6, 277, 586, 681, 794, 915
MbiI CCGCTC 1 cut(s) 756
MboI GATC 6 cut(s) 4, 275, 584, 679, 792, 913
MflI RGATCY 1 cut(s) 4
MhlI GDGCHC 2 cut(s) 487, 817
MluCI AATT 8 cut(s) 88, 157, 235, 310, 349, 970, 1023, 1055
MlyI GAGTC 2 cut(s) 1004, 1069
MmeI TCCRAC 2 cut(s) 267, 345
MroXI GAANNNNTTC 1 cut(s) 137
MseI TTAA 3 cut(s) 654, 749, 864
MslI CAYNNNNRTG 1 cut(s) 717
MspA1I CMGCKG 1 cut(s) 910
MspCI CTTAAG 1 cut(s) 863
MspI CCGG 1 cut(s) 1045
MspR9I CCNGG 4 cut(s) 112, 777, 798, 827
MvaI CCWGG 4 cut(s) 112, 777, 798, 827
MwoI GCNNNNNNNGC 1 cut(s) 206
NcoI CCATGG 1 cut(s) 210
NdeII GATC 6 cut(s) 4, 275, 584, 679, 792, 913
NlaIII CATG 4 cut(s) 214, 233, 664, 742
NlaIV GGNNCC 2 cut(s) 450, 612
NspI RCATGY 1 cut(s) 742
PasI CCCWGGG 1 cut(s) 797
PceI AGGCCT 1 cut(s) 474
PdmI GAANNNNTTC 1 cut(s) 137
PfeI GAWTC 2 cut(s) 882, 935
PflMI CCANNNNNTGG 1 cut(s) 84
PkrI GCNGC 6 cut(s) 105, 838, 841, 909, 958, 965
PleI GAGTC 2 cut(s) 1003, 1068
PpsI GAGTC 2 cut(s) 1003, 1068
Psp6I CCWGG 4 cut(s) 110, 775, 796, 825
PspFI CCCAGC 1 cut(s) 899
PspGI CCWGG 4 cut(s) 110, 775, 796, 825
PspN4I GGNNCC 2 cut(s) 450, 612
PspPI GGNCC 3 cut(s) 12, 611, 722
PstI CTGCAG 2 cut(s) 841, 965
PsuI RGATCY 1 cut(s) 4
PvuII CAGCTG 1 cut(s) 910
RseI CAYNNNNRTG 1 cut(s) 717
SaqAI TTAA 3 cut(s) 654, 749, 864
SatI GCNGC 6 cut(s) 104, 837, 840, 908, 957, 964
Sau3AI GATC 6 cut(s) 4, 275, 584, 679, 792, 913
Sau96I GGNCC 3 cut(s) 12, 611, 722
SchI GAGTC 2 cut(s) 1004, 1069
ScrFI CCNGG 4 cut(s) 112, 777, 798, 827
SduI GDGCHC 2 cut(s) 487, 817
SfaNI GCATC 1 cut(s) 418
SfcI CTRYAG 2 cut(s) 837, 961
SinI GGWCC 2 cut(s) 12, 611
SmiMI CAYNNNNRTG 1 cut(s) 717
SmlI CTYRAG 2 cut(s) 728, 863
SmoI CTYRAG 2 cut(s) 728, 863
Sse9I AATT 8 cut(s) 88, 157, 235, 310, 349, 970, 1023, 1055
SseBI AGGCCT 1 cut(s) 474
SsiI CCGC 2 cut(s) 103, 756
SspMI CTAG 2 cut(s) 470, 845
StuI AGGCCT 1 cut(s) 474
StyD4I CCNGG 4 cut(s) 110, 775, 796, 825
StyI CCWWGG 2 cut(s) 210, 439
TaaI ACNGT 1 cut(s) 568
TaqI TCGA 2 cut(s) 141, 1063
TaqII GACCGA 1 cut(s) 288
TasI AATT 8 cut(s) 88, 157, 235, 310, 349, 970, 1023, 1055
TauI GCSGC 1 cut(s) 106
TfiI GAWTC 2 cut(s) 882, 935
Tru1I TTAA 3 cut(s) 654, 749, 864
Tru9I TTAA 3 cut(s) 654, 749, 864
TseI GCWGC 5 cut(s) 836, 839, 907, 956, 963
TspDTI ATGAA 4 cut(s) 327, 677, 846, 978
TspGWI ACGGA 2 cut(s) 4, 487
Van91I CCANNNNNTGG 1 cut(s) 84
Vha464I CTTAAG 1 cut(s) 863
VpaK11BI GGWCC 2 cut(s) 12, 611
XapI RAATTY 4 cut(s) 88, 157, 970, 1023
XceI RCATGY 1 cut(s) 742
XmnI GAANNNNTTC 1 cut(s) 137
XspI CTAG 2 cut(s) 470, 845
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.