FvH4_5g33090

Uncharacterized ACR, COG1678

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0013496
Physical Location & Seq
Forward (+)
23905209 .. 23910509
5301 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g33090.t1

Sequence Viewer

Length: 1056 bp
ATGGATCTGTGGTCCGTTCACGTCACCAACAACGTCACCAGAGCCCCCTTCCTCCTCAGAAACTCCTGGAGCTGCGGCAAGGCCAGAGCTTCCTGGTGGCTCGGAGGAGGAGGAGTCGAAGACAGTGGGAGGGTGTTTGATGTTCGAGACTTGAATTTGAATTCGAAGCCGCCGCGGAGAGACGGCGGCGGCTTGATTGTGGTCAGAGCCACCGGGAAGAAGAACAACAATCAGGGAAATTCTTCGTCCTCTTCTTCTTCCGGAAATGATGACCACTCTATTCCAGAAGGAGATGGTGCAAAAGGAAACAACTCTGAAAAAATCAAAGACACTTCTTCTGAGAAAAGTTATCATATAAGTTCGGATTGGAGAGAGGTCAGAGCACAGTTTATTCGCGGGGAGCAGGCTGAAACGGATCCTCAGTACCAGTCGAAACCTCTTGGCGAGAAGTGGGCTCATCCGCTTGCAGTGCCTGAGACAGGCTGTATCCTTGTTGCAACAGAAAAGCTTGACGGAGTGCGCACCTTTGAAAGAACTGTTGTTCTCCTCCTGCGGTCAGGAAATAGACACCCAGAAGAGGGGCCATTTGGAGTTGTTATCAACAGGCCACTACACAAAAAGATTAAACACATGAGGCCCACGAATATTGATCTTGCAACCACATTCTCGGACTGCTCTCTGCATTTTGGTGGGCCTCTCGAGGCAAGCATGTTCTTGTTGAAAACAGGGGGAAAGTCGAAGCTTCCCGGGTTTGAAGAGGTGATGCCTGGTCTTTGTTTTGGTGCTCGTAACAGTCTGGATGAAGCTGCTGGTCTAGTGAAGAAGGGGGTTCTTAAGCCTCAAGATTTCAGATTTTTTGTTGGCTATGCAGGGTGGCAGCTGGACCAGTTGGCCGAGGAGATTGAATCTGATTACTGGTATGTGGCTGCTTGCAGCCCTAATTTAATATATGGAAGTTCATCAGATTCTTCGTCAGAAAGTTTGTGGGAGGAAATTTTGCAGCTAATGGGTGGCCAATATTCTGAATTGAGTCGAAAGCCTAAGCAAGATATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF179

Protein Analysis

352

Amino Acids

38.68

Weight (kDa)

6.47

Isoelectric Point (pI)

48.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF179 PF02622 175 - 337 3.5e-31 AlgH-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013496)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G33780
fragaria_vesca FvH4_5g33090
malus_domestica MD08G1215000.v1.1 MD15G1398000.v1.1
prunus_persica Prupe.1G546400_v2.0.a1
pyrus_communis pycom08g18590 pycom15g35770
rosa_chinensis RchiOBHm_Chr7g0231261
rosa_laevigata RLG00000001423
rosa_multiflora Rmu_sc0003396.1_g000004
rosa_roxburghii Rroxscaffold_3G00229370
rosa_rugosa Rorug07G0265600 Rorug07G0265600
rosa_samantha Rh7AG419300 Rh7BG394500 Rh7CG439000 Rh7DG412900
rosa_wichuraiana Rw7G034660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 521
AccII CGCG 2 cut(s) 175, 396
AccIII TCCGGA 1 cut(s) 260
AciI CCGC 9 cut(s) 75, 170, 173, 175, 186, 189, 396, 461, 553
AclWI GGATC 3 cut(s) 12, 410, 423
AcoI YGGCCR 2 cut(s) 891, 1012
AcsI RAATTY 4 cut(s) 154, 160, 238, 993
AfaI GTAC 1 cut(s) 425
AfiI CCNNNNNNNGG 3 cut(s) 479, 577, 578
AflII CTTAAG 1 cut(s) 833
AgsI TTSAA 6 cut(s) 154, 160, 530, 721, 755, 905
AjiI CACGTC 1 cut(s) 22
AjnI CCWGG 3 cut(s) 65, 92, 766
AluBI AGCT 7 cut(s) 72, 89, 508, 742, 806, 880, 1003
AluI AGCT 7 cut(s) 72, 89, 508, 742, 806, 880, 1003
Alw21I GWGCWC 2 cut(s) 385, 787
Alw26I GTCTC 3 cut(s) 141, 174, 470
AlwI GGATC 3 cut(s) 12, 410, 423
AlwNI CAGNNNCTG 1 cut(s) 473
Ama87I CYCGRG 2 cut(s) 698, 746
Aor13HI TCCGGA 1 cut(s) 260
AoxI GGCC 7 cut(s) 81, 581, 605, 635, 692, 891, 1012
ApeKI GCWGC 6 cut(s) 72, 806, 877, 926, 933, 1000
ApoI RAATTY 4 cut(s) 154, 160, 238, 993
Asp700I GAANNNNTTC 1 cut(s) 241
AspLEI GCGC 1 cut(s) 522
AspS9I GGNCC 5 cut(s) 12, 581, 636, 692, 883
AsuC2I CCSGG 3 cut(s) 214, 747, 748
AsuHPI GGTGA 3 cut(s) 16, 28, 772
AsuII TTCGAA 1 cut(s) 164
AvaI CYCGRG 2 cut(s) 698, 746
AvaII GGWCC 2 cut(s) 12, 883
BalI TGGCCA 1 cut(s) 1014
BamHI GGATCC 1 cut(s) 415
BanII GRGCYC 2 cut(s) 46, 457
BbsI GAAGAC 1 cut(s) 126
Bbv12I GWGCWC 2 cut(s) 385, 787
BbvI GCAGC 6 cut(s) 59, 793, 889, 913, 945, 1012
BccI CCATC 1 cut(s) 287
BceAI ACGGC 1 cut(s) 199
BciT130I CCWGG 3 cut(s) 67, 94, 768
BciVI GTATCC 1 cut(s) 497
BcnI CCSGG 3 cut(s) 214, 747, 748
BcoDI GTCTC 3 cut(s) 141, 174, 470
BfaI CTAG 1 cut(s) 815
BfrI CTTAAG 1 cut(s) 833
BfuI GTATCC 1 cut(s) 497
Bme1390I CCNGG 6 cut(s) 67, 94, 214, 747, 748, 768
Bme18I GGWCC 2 cut(s) 12, 883
BmeT110I CYCGRG 2 cut(s) 698, 746
BmgBI CACGTC 1 cut(s) 22
BmgT120I GGNCC 5 cut(s) 12, 581, 636, 692, 883
BmiI GGNNCC 2 cut(s) 417, 582
BmrFI CCNGG 6 cut(s) 67, 94, 214, 747, 748, 768
BmsI GCATC 1 cut(s) 753
BpiI GAAGAC 1 cut(s) 126
BpmI CTGGAG 1 cut(s) 88
Bpu10I CCTNAGC 1 cut(s) 1041
Bpu14I TTCGAA 1 cut(s) 164
BpuEI CTTGAG 1 cut(s) 825
BpuMI CCSGG 3 cut(s) 214, 747, 748
BsaJI CCNNGG 3 cut(s) 173, 746, 894
BsaWI WCCGGW 1 cut(s) 260
BsaXI ACNNNNNCTCC 2 cut(s) 99, 129
Bsc4I CCNNNNNNNGG 3 cut(s) 479, 577, 578
Bse1I ACTGG 3 cut(s) 427, 886, 920
BseAI TCCGGA 1 cut(s) 260
BseBI CCWGG 3 cut(s) 67, 94, 768
BseDI CCNNGG 3 cut(s) 173, 746, 894
BseGI GGATG 2 cut(s) 457, 805
BseLI CCNNNNNNNGG 3 cut(s) 479, 577, 578
BseMII CTCAG 4 cut(s) 70, 330, 434, 465
BseNI ACTGG 3 cut(s) 427, 886, 920
BseRI GAGGAG 6 cut(s) 44, 120, 123, 126, 536, 911
BseXI GCAGC 6 cut(s) 59, 793, 889, 913, 945, 1012
Bsh1236I CGCG 2 cut(s) 175, 396
BshFI GGCC 7 cut(s) 83, 583, 607, 637, 694, 893, 1014
BsiHKAI GWGCWC 2 cut(s) 385, 787
BsiHKCI CYCGRG 2 cut(s) 698, 746
BsiSI CCGG 3 cut(s) 213, 261, 747
BslI CCNNNNNNNGG 3 cut(s) 479, 577, 578
BsmAI GTCTC 3 cut(s) 141, 174, 470
BsmBI CGTCTC 1 cut(s) 174
BsnI GGCC 7 cut(s) 83, 583, 607, 637, 694, 893, 1014
BsoBI CYCGRG 2 cut(s) 698, 746
Bsp119I TTCGAA 1 cut(s) 164
Bsp1286I GDGCHC 4 cut(s) 46, 385, 457, 787
Bsp13I TCCGGA 1 cut(s) 260
Bsp143I GATC 3 cut(s) 4, 415, 649
BspACI CCGC 9 cut(s) 75, 170, 173, 175, 186, 189, 396, 461, 553
BspANI GGCC 7 cut(s) 83, 583, 607, 637, 694, 893, 1014
BspCNI CTCAG 4 cut(s) 69, 331, 433, 466
BspEI TCCGGA 1 cut(s) 260
BspFNI CGCG 2 cut(s) 175, 396
BspLI GGNNCC 2 cut(s) 417, 582
BspPI GGATC 3 cut(s) 12, 410, 423
BspT104I TTCGAA 1 cut(s) 164
BspTI CTTAAG 1 cut(s) 833
BsrI ACTGG 3 cut(s) 427, 886, 920
BssECI CCNNGG 3 cut(s) 173, 746, 894
BssMI GATC 3 cut(s) 4, 415, 649
Bst2UI CCWGG 3 cut(s) 67, 94, 768
Bst4CI ACNGT 4 cut(s) 125, 387, 538, 794
Bst6I CTCTTC 3 cut(s) 256, 570, 750
BstAFI CTTAAG 1 cut(s) 833
BstBI TTCGAA 1 cut(s) 164
BstC8I GCNNGC 4 cut(s) 405, 465, 706, 931
BstDEI CTNAG 5 cut(s) 56, 339, 420, 474, 1041
BstDSI CCRYGG 1 cut(s) 173
BstENI CCTNNNNNAGG 1 cut(s) 477
BstF5I GGATG 2 cut(s) 457, 805
BstFNI CGCG 2 cut(s) 175, 396
BstHHI GCGC 1 cut(s) 522
BstKTI GATC 3 cut(s) 7, 418, 652
BstMAI GTCTC 3 cut(s) 141, 174, 470
BstMBI GATC 3 cut(s) 4, 415, 649
BstMWI GCNNNNNNNGC 1 cut(s) 469
BstNI CCWGG 3 cut(s) 67, 94, 768
BstNSI RCATGY 1 cut(s) 712
BstSCI CCNGG 6 cut(s) 65, 92, 212, 745, 746, 766
BstUI CGCG 2 cut(s) 175, 396
BstV1I GCAGC 6 cut(s) 59, 793, 889, 913, 945, 1012
BstV2I GAAGAC 1 cut(s) 126
BstX2I RGATCY 2 cut(s) 4, 415
BstYI RGATCY 2 cut(s) 4, 415
BsuI GTATCC 1 cut(s) 497
BsuRI GGCC 7 cut(s) 83, 583, 607, 637, 694, 893, 1014
BtgI CCRYGG 1 cut(s) 173
BtrI CACGTC 1 cut(s) 22
BtsCI GGATG 2 cut(s) 457, 805
BtsI GCAGTG 1 cut(s) 474
BtsIMutI CAGTG 2 cut(s) 130, 474
Cac8I GCNNGC 4 cut(s) 405, 465, 706, 931
CaiI CAGNNNCTG 1 cut(s) 473
CfoI GCGC 1 cut(s) 522
Cfr13I GGNCC 5 cut(s) 12, 581, 636, 692, 883
Cfr42I CCGCGG 1 cut(s) 176
Cfr9I CCCGGG 1 cut(s) 746
Csp6I GTAC 1 cut(s) 424
CspCI CAANNNNNGTGG 2 cut(s) 628, 663
CviAII CATG 2 cut(s) 631, 709
CviQI GTAC 1 cut(s) 424
DdeI CTNAG 5 cut(s) 56, 339, 420, 474, 1041
DpnI GATC 3 cut(s) 6, 417, 651
DpnII GATC 3 cut(s) 4, 415, 649
EaeI YGGCCR 2 cut(s) 891, 1012
Eam1104I CTCTTC 3 cut(s) 256, 570, 750
EarI CTCTTC 3 cut(s) 256, 570, 750
Eco24I GRGCYC 2 cut(s) 46, 457
Eco47I GGWCC 2 cut(s) 12, 883
Eco88I CYCGRG 2 cut(s) 698, 746
EcoNI CCTNNNNNAGG 1 cut(s) 477
EcoRI GAATTC 1 cut(s) 160
EcoRII CCWGG 3 cut(s) 65, 92, 766
EcoT38I GRGCYC 2 cut(s) 46, 457
Esp3I CGTCTC 1 cut(s) 174
FaeI CATG 2 cut(s) 634, 712
FaiI YATR 9 cut(s) 354, 356, 632, 710, 867, 921, 949, 951, 1052
FatI CATG 2 cut(s) 630, 708
FauI CCCGC 1 cut(s) 389
FokI GGATG 2 cut(s) 444, 812
FriOI GRGCYC 2 cut(s) 46, 457
FspAI RTGCGCAY 1 cut(s) 521
FspBI CTAG 1 cut(s) 815
FspI TGCGCA 1 cut(s) 521
GlaI GCGC 1 cut(s) 521
GsuI CTGGAG 1 cut(s) 88
HaeIII GGCC 7 cut(s) 83, 583, 607, 637, 694, 893, 1014
HapII CCGG 3 cut(s) 213, 261, 747
HhaI GCGC 1 cut(s) 522
Hin1II CATG 2 cut(s) 634, 712
Hin6I GCGC 1 cut(s) 520
HinP1I GCGC 1 cut(s) 520
HindIII AAGCTT 2 cut(s) 506, 740
HinfI GANTC 4 cut(s) 114, 905, 965, 1030
HpaII CCGG 3 cut(s) 213, 261, 747
HphI GGTGA 3 cut(s) 16, 28, 772
Hpy166II GTNNAC 1 cut(s) 19
Hpy188III TCNNGA 7 cut(s) 146, 261, 284, 558, 698, 797, 842
Hpy8I GTNNAC 1 cut(s) 19
HpyAV CCTTC 3 cut(s) 58, 281, 817
HpyCH4III ACNGT 4 cut(s) 125, 387, 538, 794
HpyCH4IV ACGT 2 cut(s) 21, 33
HpyCH4V TGCA 8 cut(s) 299, 467, 497, 656, 682, 869, 933, 1000
HpyF10VI GCNNNNNNNGC 1 cut(s) 469
HpyF3I CTNAG 5 cut(s) 56, 339, 420, 474, 1041
HpySE526I ACGT 2 cut(s) 21, 33
Hsp92II CATG 2 cut(s) 634, 712
HspAI GCGC 1 cut(s) 520
Kpn2I TCCGGA 1 cut(s) 260
KspI CCGCGG 1 cut(s) 176
Kzo9I GATC 3 cut(s) 4, 415, 649
LmnI GCTCC 2 cut(s) 69, 400
Lsp1109I GCAGC 6 cut(s) 59, 793, 889, 913, 945, 1012
LweI GCATC 1 cut(s) 753
MaeI CTAG 1 cut(s) 815
MaeII ACGT 2 cut(s) 21, 33
MaeIII GTNAC 3 cut(s) 22, 34, 788
MalI GATC 3 cut(s) 6, 417, 651
MboI GATC 3 cut(s) 4, 415, 649
MflI RGATCY 2 cut(s) 4, 415
MhlI GDGCHC 4 cut(s) 46, 385, 457, 787
MlsI TGGCCA 1 cut(s) 1014
MluCI AATT 6 cut(s) 154, 160, 238, 940, 993, 1025
MluNI TGGCCA 1 cut(s) 1014
MlyI GAGTC 2 cut(s) 123, 1039
Mox20I TGGCCA 1 cut(s) 1014
MroI TCCGGA 1 cut(s) 260
MroXI GAANNNNTTC 1 cut(s) 241
MscI TGGCCA 1 cut(s) 1014
MseI TTAA 3 cut(s) 624, 834, 944
MslI CAYNNNNRTG 1 cut(s) 687
Msp20I TGGCCA 1 cut(s) 1014
MspA1I CMGCKG 2 cut(s) 175, 880
MspCI CTTAAG 1 cut(s) 833
MspI CCGG 3 cut(s) 213, 261, 747
MspR9I CCNGG 6 cut(s) 67, 94, 214, 747, 748, 768
MvaI CCWGG 3 cut(s) 67, 94, 768
MvnI CGCG 2 cut(s) 175, 396
MwoI GCNNNNNNNGC 1 cut(s) 469
NciI CCSGG 3 cut(s) 214, 747, 748
NdeII GATC 3 cut(s) 4, 415, 649
NlaIII CATG 2 cut(s) 634, 712
NlaIV GGNNCC 2 cut(s) 417, 582
NmeAIII GCCGAG 1 cut(s) 919
NmuCI GTSAC 2 cut(s) 22, 34
NsbI TGCGCA 1 cut(s) 521
NspI RCATGY 1 cut(s) 712
NspV TTCGAA 1 cut(s) 164
PaeR7I CTCGAG 1 cut(s) 698
PdmI GAANNNNTTC 1 cut(s) 241
PfeI GAWTC 2 cut(s) 905, 965
PfoI TCCNGGA 1 cut(s) 65
PleI GAGTC 2 cut(s) 122, 1038
PpsI GAGTC 2 cut(s) 122, 1038
Psp6I CCWGG 3 cut(s) 65, 92, 766
PspGI CCWGG 3 cut(s) 65, 92, 766
PspN4I GGNNCC 2 cut(s) 417, 582
PspPI GGNCC 5 cut(s) 12, 581, 636, 692, 883
PstNI CAGNNNCTG 1 cut(s) 473
PsuI RGATCY 2 cut(s) 4, 415
PvuII CAGCTG 1 cut(s) 880
RsaI GTAC 1 cut(s) 425
RsaNI GTAC 1 cut(s) 424
RseI CAYNNNNRTG 1 cut(s) 687
SacII CCGCGG 1 cut(s) 176
SaqAI TTAA 3 cut(s) 624, 834, 944
Sau3AI GATC 3 cut(s) 4, 415, 649
Sau96I GGNCC 5 cut(s) 12, 581, 636, 692, 883
SchI GAGTC 2 cut(s) 123, 1039
ScrFI CCNGG 6 cut(s) 67, 94, 214, 747, 748, 768
SduI GDGCHC 4 cut(s) 46, 385, 457, 787
SfaNI GCATC 1 cut(s) 753
Sfr274I CTCGAG 1 cut(s) 698
Sfr303I CCGCGG 1 cut(s) 176
SfuI TTCGAA 1 cut(s) 164
SgrBI CCGCGG 1 cut(s) 176
SinI GGWCC 2 cut(s) 12, 883
SlaI CTCGAG 1 cut(s) 698
SmaI CCCGGG 1 cut(s) 748
SmiMI CAYNNNNRTG 1 cut(s) 687
SmlI CTYRAG 3 cut(s) 698, 833, 840
SmoI CTYRAG 3 cut(s) 698, 833, 840
Sse9I AATT 6 cut(s) 154, 160, 238, 940, 993, 1025
SsiI CCGC 9 cut(s) 75, 170, 173, 175, 186, 189, 396, 461, 553
SspI AATATT 2 cut(s) 646, 1019
SspMI CTAG 1 cut(s) 815
StyD4I CCNGG 6 cut(s) 65, 92, 212, 745, 746, 766
TaaI ACNGT 4 cut(s) 125, 387, 538, 794
TaiI ACGT 2 cut(s) 24, 36
TaqI TCGA 7 cut(s) 117, 145, 164, 431, 699, 737, 1033
TasI AATT 6 cut(s) 154, 160, 238, 940, 993, 1025
TauI GCSGC 5 cut(s) 78, 172, 175, 189, 192
TfiI GAWTC 2 cut(s) 905, 965
Tru1I TTAA 3 cut(s) 624, 834, 944
Tru9I TTAA 3 cut(s) 624, 834, 944
TscAI CASTG 2 cut(s) 130, 474
TseFI GTSAC 2 cut(s) 22, 34
TseI GCWGC 6 cut(s) 72, 806, 877, 926, 933, 1000
Tsp45I GTSAC 2 cut(s) 22, 34
TspDTI ATGAA 2 cut(s) 816, 948
TspGWI ACGGA 3 cut(s) 4, 428, 528
TspMI CCCGGG 1 cut(s) 746
TspRI CASTG 2 cut(s) 130, 474
Vha464I CTTAAG 1 cut(s) 833
VpaK11BI GGWCC 2 cut(s) 12, 883
XagI CCTNNNNNAGG 1 cut(s) 477
XapI RAATTY 4 cut(s) 154, 160, 238, 993
XceI RCATGY 1 cut(s) 712
XhoI CTCGAG 1 cut(s) 698
XmaI CCCGGG 1 cut(s) 746
XmnI GAANNNNTTC 1 cut(s) 241
XspI CTAG 1 cut(s) 815
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.