MD15G1398000.v1.1

Uncharacterized ACR, COG1678

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0013496
Physical Location & Seq
Reverse (-)
49841984 .. 49851768
9785 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1398000.v1.1.491

Sequence Viewer

Length: 1065 bp
ATGGATCTGTGGTCCGTGCAGGCGAACAACATCGGCAGAAGCCCTTTTCTGCTCAGAAACTCGTTTTCTGAGAAACCCATTTCGTGGAGTTTTGTAAATGTGCGACCTTCCTGGGTTGGCGTTGAAGATGGGAAGGATTTCGAGATTGGGGTCTTGAATTGGAAGCGCAGGATTTCGTCTAAAGGGTTTCGTTCTTCGGTTGTCAGAGCCATGGGGAAGAAGAACAATAATGGAAATTCGTCGAACTCTTCGTCTTCGTCAGGAAATGGTGACCGCTCTATTCCAGAAGGTGATAGTGAACAAGAAAACTATTCTTCTGAAAACTTCAGTGACGCTTCATCCTCTGACTGGAGAGAGGTCAGAGCAAGGCTGTATACTATAGAGCAGGAAGAAAATGCTGAGTCTGATGCTCAGAACCAAGGTGGGGCACCCCACCAGTCGAAACCTCTTGGCCTGAAGTGGGCTCATCCTATTTCCGTACCAGAGACAGGCTGTGTCCTTGTTGCCACAGAAAAGCTTGACGGGGTTCGTACCTTTGAAAGAACTGTTGTTCTCCTCCTCAGATCAGGAACCAGACACCCAGAAGAGGGACCATTTGGAGTTGTTATCAACAGGCCACTCCATAAAAAGATTAAACACATGAAGCCCACGAATCCTGATCTTGCAACCACATTTTCTGATTGTTCGTTGCATTTTGGTGGGCCTCTTGAGGCAAGCATGTTTTTGTTAAAAAGCGGGGGCAAGTCAAAGCTCCCTGGGTTTGAAGAGGTGATCCCTGGCCTTTGTTTTGGTGCTCGTAACAGCCTGGATGAAGCTGCAGGGTTAGTAAAGAAGGGTGTTCTTAAGCCTCAAGATTTCAGATTCTTTGTTGGCTATGCTGGGTGGCAGCTGGATCAGTTGGCAGAGGAGATTGAATCGGATTATTGGTACGTGGCTGCCTGCAGCGCAAATTTGATTTTTGGGGCTTCATCGGAGTCCTCACAAGAAAGTTTGTGGGAAGAAATTTTGCAGGAAATGGGTGGCCAGTATTCTGAAGTGAGTCGAAAGCCGAAGCAGGATATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF179

Protein Analysis

355

Amino Acids

39.15

Weight (kDa)

5.68

Isoelectric Point (pI)

53.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF179 PF02622 178 - 340 2.1e-31 AlgH-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013496)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G33780
fragaria_vesca FvH4_5g33090
malus_domestica MD08G1215000.v1.1 MD15G1398000.v1.1
prunus_persica Prupe.1G546400_v2.0.a1
pyrus_communis pycom08g18590 pycom15g35770
rosa_chinensis RchiOBHm_Chr7g0231261
rosa_laevigata RLG00000001423
rosa_multiflora Rmu_sc0003396.1_g000004
rosa_roxburghii Rroxscaffold_3G00229370
rosa_rugosa Rorug07G0265600 Rorug07G0265600
rosa_samantha Rh7AG419300 Rh7BG394500 Rh7CG439000 Rh7DG412900
rosa_wichuraiana Rw7G034660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 427
AccB7I CCANNNNNTGG 1 cut(s) 84
AccBSI CCGCTC 1 cut(s) 276
AccI GTMKAC 1 cut(s) 374
AciI CCGC 2 cut(s) 274, 735
AclWI GGATC 3 cut(s) 12, 766, 900
AcoI YGGCCR 1 cut(s) 1021
AcsI RAATTY 3 cut(s) 235, 949, 1002
AcuI CTGAAG 3 cut(s) 310, 476, 1053
AfaI GTAC 3 cut(s) 480, 532, 929
AfiI CCNNNNNNNGG 7 cut(s) 84, 348, 424, 460, 488, 586, 587
AflII CTTAAG 1 cut(s) 842
AgsI TTSAA 5 cut(s) 125, 157, 539, 764, 914
AjnI CCWGG 4 cut(s) 110, 754, 775, 804
AluBI AGCT 4 cut(s) 517, 751, 815, 889
AluI AGCT 4 cut(s) 517, 751, 815, 889
Alw21I GWGCWC 1 cut(s) 796
Alw26I GTCTC 1 cut(s) 479
AlwI GGATC 3 cut(s) 12, 766, 900
AoxI GGCC 5 cut(s) 451, 614, 701, 778, 1021
ApeKI GCWGC 4 cut(s) 815, 886, 935, 942
ApoI RAATTY 3 cut(s) 235, 949, 1002
ArsI GACNNNNNNTTYG 2 cut(s) 236, 268
Asp700I GAANNNNTTC 1 cut(s) 137
AspLEI GCGC 2 cut(s) 168, 947
AspS9I GGNCC 3 cut(s) 12, 590, 701
AsuHPI GGTGA 3 cut(s) 281, 302, 781
AvaII GGWCC 2 cut(s) 12, 590
BaeGI GKGCMC 1 cut(s) 430
BalI TGGCCA 1 cut(s) 1023
BanI GGYRCC 1 cut(s) 427
BanII GRGCYC 1 cut(s) 466
BbsI GAAGAC 1 cut(s) 246
Bbv12I GWGCWC 1 cut(s) 796
BbvI GCAGC 4 cut(s) 802, 898, 922, 954
BccI CCATC 1 cut(s) 122
BciT130I CCWGG 4 cut(s) 112, 756, 777, 806
BcoDI GTCTC 1 cut(s) 479
BfmI CTRYAG 3 cut(s) 378, 816, 940
BfrI CTTAAG 1 cut(s) 842
BisI GCNGC 4 cut(s) 816, 887, 936, 943
BlsI GCNGC 4 cut(s) 817, 888, 937, 944
Bme1390I CCNGG 4 cut(s) 112, 756, 777, 806
Bme18I GGWCC 2 cut(s) 12, 590
BmgT120I GGNCC 3 cut(s) 12, 590, 701
BmiI GGNNCC 3 cut(s) 429, 571, 591
BmrFI CCNGG 4 cut(s) 112, 756, 777, 806
BmsI GCATC 1 cut(s) 397
BpiI GAAGAC 1 cut(s) 246
BpmI CTGGAG 1 cut(s) 370
BpuEI CTTGAG 2 cut(s) 728, 834
BsaAI YACGTR 1 cut(s) 931
BsaJI CCNNGG 6 cut(s) 111, 210, 418, 754, 755, 775
Bsc4I CCNNNNNNNGG 7 cut(s) 84, 348, 424, 460, 488, 586, 587
Bse1I ACTGG 3 cut(s) 353, 436, 1024
BseBI CCWGG 4 cut(s) 112, 756, 777, 806
BseDI CCNNGG 6 cut(s) 111, 210, 418, 754, 755, 775
BseGI GGATG 3 cut(s) 338, 466, 814
BseLI CCNNNNNNNGG 7 cut(s) 84, 348, 424, 460, 488, 586, 587
BseMII CTCAG 5 cut(s) 60, 67, 390, 425, 574
BseNI ACTGG 3 cut(s) 353, 436, 1024
BseRI GAGGAG 3 cut(s) 545, 548, 920
BseSI GKGCMC 1 cut(s) 430
BseXI GCAGC 4 cut(s) 802, 898, 922, 954
BseYI CCCAGC 1 cut(s) 878
BsgI GTGCAG 1 cut(s) 38
BshFI GGCC 5 cut(s) 453, 616, 703, 780, 1023
BshNI GGYRCC 1 cut(s) 427
BsiHKAI GWGCWC 1 cut(s) 796
BslFI GGGAC 1 cut(s) 603
BslI CCNNNNNNNGG 7 cut(s) 84, 348, 424, 460, 488, 586, 587
BsmAI GTCTC 1 cut(s) 479
BsmFI GGGAC 1 cut(s) 603
BsnI GGCC 5 cut(s) 453, 616, 703, 780, 1023
Bsp1286I GDGCHC 3 cut(s) 430, 466, 796
Bsp143I GATC 5 cut(s) 4, 563, 658, 771, 892
Bsp19I CCATGG 1 cut(s) 210
BspACI CCGC 2 cut(s) 274, 735
BspANI GGCC 5 cut(s) 453, 616, 703, 780, 1023
BspCNI CTCAG 5 cut(s) 61, 66, 391, 424, 573
BspLI GGNNCC 3 cut(s) 429, 571, 591
BspMAI CTGCAG 2 cut(s) 820, 944
BspPI GGATC 3 cut(s) 12, 766, 900
BspT107I GGYRCC 1 cut(s) 427
BspTI CTTAAG 1 cut(s) 842
BsrBI CCGCTC 1 cut(s) 276
BsrI ACTGG 3 cut(s) 353, 436, 1024
BssECI CCNNGG 6 cut(s) 111, 210, 418, 754, 755, 775
BssMI GATC 5 cut(s) 4, 563, 658, 771, 892
BssNAI GTATAC 1 cut(s) 375
BssT1I CCWWGG 2 cut(s) 210, 418
Bst1107I GTATAC 1 cut(s) 375
Bst2UI CCWGG 4 cut(s) 112, 756, 777, 806
Bst4CI ACNGT 1 cut(s) 547
Bst6I CTCTTC 3 cut(s) 253, 579, 759
BstAFI CTTAAG 1 cut(s) 842
BstBAI YACGTR 1 cut(s) 931
BstC8I GCNNGC 3 cut(s) 21, 715, 940
BstDEI CTNAG 5 cut(s) 53, 69, 399, 411, 560
BstDSI CCRYGG 1 cut(s) 210
BstEII GGTNACC 1 cut(s) 269
BstF5I GGATG 3 cut(s) 338, 466, 814
BstHHI GCGC 2 cut(s) 168, 947
BstKTI GATC 5 cut(s) 7, 566, 661, 774, 895
BstMAI GTCTC 1 cut(s) 479
BstMBI GATC 5 cut(s) 4, 563, 658, 771, 892
BstMWI GCNNNNNNNGC 1 cut(s) 944
BstNI CCWGG 4 cut(s) 112, 756, 777, 806
BstNSI RCATGY 1 cut(s) 721
BstPI GGTNACC 1 cut(s) 269
BstSCI CCNGG 4 cut(s) 110, 754, 775, 804
BstSFI CTRYAG 3 cut(s) 378, 816, 940
BstSLI GKGCMC 1 cut(s) 430
BstV1I GCAGC 4 cut(s) 802, 898, 922, 954
BstV2I GAAGAC 1 cut(s) 246
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
BstZ17I GTATAC 1 cut(s) 375
BsuRI GGCC 5 cut(s) 453, 616, 703, 780, 1023
BtgI CCRYGG 1 cut(s) 210
BtsCI GGATG 3 cut(s) 338, 466, 814
BtsIMutI CAGTG 1 cut(s) 334
Cac8I GCNNGC 3 cut(s) 21, 715, 940
CfoI GCGC 2 cut(s) 168, 947
Cfr13I GGNCC 3 cut(s) 12, 590, 701
CseI GACGC 1 cut(s) 341
Csp6I GTAC 3 cut(s) 479, 531, 928
CviAII CATG 3 cut(s) 211, 640, 718
CviQI GTAC 3 cut(s) 479, 531, 928
DdeI CTNAG 5 cut(s) 53, 69, 399, 411, 560
DpnI GATC 5 cut(s) 6, 565, 660, 773, 894
DpnII GATC 5 cut(s) 4, 563, 658, 771, 892
EaeI YGGCCR 1 cut(s) 1021
Eam1104I CTCTTC 3 cut(s) 253, 579, 759
EarI CTCTTC 3 cut(s) 253, 579, 759
Eco130I CCWWGG 2 cut(s) 210, 418
Eco24I GRGCYC 1 cut(s) 466
Eco47I GGWCC 2 cut(s) 12, 590
Eco57I CTGAAG 3 cut(s) 310, 476, 1053
Eco91I GGTNACC 1 cut(s) 269
EcoO65I GGTNACC 1 cut(s) 269
EcoRII CCWGG 4 cut(s) 110, 754, 775, 804
EcoT14I CCWWGG 2 cut(s) 210, 418
EcoT38I GRGCYC 1 cut(s) 466
ErhI CCWWGG 2 cut(s) 210, 418
FaeI CATG 3 cut(s) 214, 643, 721
FaiI YATR 8 cut(s) 212, 375, 380, 624, 641, 719, 876, 1061
FaqI GGGAC 1 cut(s) 603
FatI CATG 3 cut(s) 210, 639, 717
FauI CCCGC 1 cut(s) 728
FblI GTMKAC 1 cut(s) 374
Fnu4HI GCNGC 4 cut(s) 816, 887, 936, 943
FokI GGATG 3 cut(s) 325, 453, 821
FriOI GRGCYC 1 cut(s) 466
Fsp4HI GCNGC 4 cut(s) 816, 887, 936, 943
GlaI GCGC 2 cut(s) 167, 946
GluI GCNGC 4 cut(s) 816, 887, 936, 943
GsaI CCCAGC 1 cut(s) 882
GsuI CTGGAG 1 cut(s) 370
HaeIII GGCC 5 cut(s) 453, 616, 703, 780, 1023
HgaI GACGC 1 cut(s) 341
HhaI GCGC 2 cut(s) 168, 947
Hin1II CATG 3 cut(s) 214, 643, 721
Hin6I GCGC 2 cut(s) 166, 945
HinP1I GCGC 2 cut(s) 166, 945
HindIII AAGCTT 1 cut(s) 515
HinfI GANTC 6 cut(s) 401, 652, 861, 914, 974, 1039
HphI GGTGA 3 cut(s) 281, 302, 781
Hpy166II GTNNAC 2 cut(s) 299, 375
Hpy188III TCNNGA 8 cut(s) 142, 154, 261, 284, 567, 656, 707, 851
Hpy8I GTNNAC 2 cut(s) 299, 375
Hpy99I CGWCG 1 cut(s) 244
HpyAV CCTTC 4 cut(s) 117, 127, 281, 826
HpyCH4III ACNGT 1 cut(s) 547
HpyCH4IV ACGT 1 cut(s) 930
HpyCH4V TGCA 6 cut(s) 19, 665, 691, 818, 942, 1009
HpyF10VI GCNNNNNNNGC 1 cut(s) 944
HpyF3I CTNAG 5 cut(s) 53, 69, 399, 411, 560
HpySE526I ACGT 1 cut(s) 930
Hsp92II CATG 3 cut(s) 214, 643, 721
HspAI GCGC 2 cut(s) 166, 945
Kzo9I GATC 5 cut(s) 4, 563, 658, 771, 892
LmnI GCTCC 1 cut(s) 756
Lsp1109I GCAGC 4 cut(s) 802, 898, 922, 954
LweI GCATC 1 cut(s) 397
MaeII ACGT 1 cut(s) 930
MaeIII GTNAC 3 cut(s) 269, 329, 797
MalI GATC 5 cut(s) 6, 565, 660, 773, 894
MbiI CCGCTC 1 cut(s) 276
MboI GATC 5 cut(s) 4, 563, 658, 771, 892
MflI RGATCY 1 cut(s) 4
MhlI GDGCHC 3 cut(s) 430, 466, 796
MlsI TGGCCA 1 cut(s) 1023
MluCI AATT 4 cut(s) 157, 235, 949, 1002
MluNI TGGCCA 1 cut(s) 1023
MlyI GAGTC 3 cut(s) 410, 983, 1048
Mox20I TGGCCA 1 cut(s) 1023
MroXI GAANNNNTTC 1 cut(s) 137
MscI TGGCCA 1 cut(s) 1023
MseI TTAA 3 cut(s) 633, 728, 843
MslI CAYNNNNRTG 1 cut(s) 696
Msp20I TGGCCA 1 cut(s) 1023
MspA1I CMGCKG 1 cut(s) 889
MspCI CTTAAG 1 cut(s) 842
MspR9I CCNGG 4 cut(s) 112, 756, 777, 806
MvaI CCWGG 4 cut(s) 112, 756, 777, 806
MwoI GCNNNNNNNGC 1 cut(s) 944
NcoI CCATGG 1 cut(s) 210
NdeII GATC 5 cut(s) 4, 563, 658, 771, 892
NlaIII CATG 3 cut(s) 214, 643, 721
NlaIV GGNNCC 3 cut(s) 429, 571, 591
NmuCI GTSAC 2 cut(s) 269, 329
NspI RCATGY 1 cut(s) 721
PasI CCCWGGG 1 cut(s) 755
PcsI WCGNNNNNNNCGW 1 cut(s) 248
PdmI GAANNNNTTC 1 cut(s) 137
PfeI GAWTC 3 cut(s) 652, 861, 914
PflMI CCANNNNNTGG 1 cut(s) 84
PkrI GCNGC 4 cut(s) 817, 888, 937, 944
PleI GAGTC 3 cut(s) 409, 982, 1047
PpsI GAGTC 3 cut(s) 409, 982, 1047
Ppu21I YACGTR 1 cut(s) 931
Psp6I CCWGG 4 cut(s) 110, 754, 775, 804
PspEI GGTNACC 1 cut(s) 269
PspFI CCCAGC 1 cut(s) 878
PspGI CCWGG 4 cut(s) 110, 754, 775, 804
PspN4I GGNNCC 3 cut(s) 429, 571, 591
PspPI GGNCC 3 cut(s) 12, 590, 701
PstI CTGCAG 2 cut(s) 820, 944
PsuI RGATCY 1 cut(s) 4
PvuII CAGCTG 1 cut(s) 889
RsaI GTAC 3 cut(s) 480, 532, 929
RsaNI GTAC 3 cut(s) 479, 531, 928
RseI CAYNNNNRTG 1 cut(s) 696
SaqAI TTAA 3 cut(s) 633, 728, 843
SatI GCNGC 4 cut(s) 816, 887, 936, 943
Sau3AI GATC 5 cut(s) 4, 563, 658, 771, 892
Sau96I GGNCC 3 cut(s) 12, 590, 701
SchI GAGTC 3 cut(s) 410, 983, 1048
ScrFI CCNGG 4 cut(s) 112, 756, 777, 806
SduI GDGCHC 3 cut(s) 430, 466, 796
SfaNI GCATC 1 cut(s) 397
SfcI CTRYAG 3 cut(s) 378, 816, 940
SinI GGWCC 2 cut(s) 12, 590
SmiMI CAYNNNNRTG 1 cut(s) 696
SmlI CTYRAG 3 cut(s) 707, 842, 849
SmoI CTYRAG 3 cut(s) 707, 842, 849
Sse9I AATT 4 cut(s) 157, 235, 949, 1002
SsiI CCGC 2 cut(s) 274, 735
StyD4I CCNGG 4 cut(s) 110, 754, 775, 804
StyI CCWWGG 2 cut(s) 210, 418
TaaI ACNGT 1 cut(s) 547
TaiI ACGT 1 cut(s) 933
TaqI TCGA 4 cut(s) 141, 242, 440, 1042
TasI AATT 4 cut(s) 157, 235, 949, 1002
TfiI GAWTC 3 cut(s) 652, 861, 914
Tru1I TTAA 3 cut(s) 633, 728, 843
Tru9I TTAA 3 cut(s) 633, 728, 843
TscAI CASTG 1 cut(s) 334
TseFI GTSAC 2 cut(s) 269, 329
TseI GCWGC 4 cut(s) 815, 886, 935, 942
Tsp45I GTSAC 2 cut(s) 269, 329
TspDTI ATGAA 4 cut(s) 327, 656, 825, 957
TspGWI ACGGA 2 cut(s) 4, 466
TspRI CASTG 1 cut(s) 334
Van91I CCANNNNNTGG 1 cut(s) 84
Vha464I CTTAAG 1 cut(s) 842
VpaK11BI GGWCC 2 cut(s) 12, 590
XapI RAATTY 3 cut(s) 235, 949, 1002
XceI RCATGY 1 cut(s) 721
XmiI GTMKAC 1 cut(s) 374
XmnI GAANNNNTTC 1 cut(s) 137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.