AT1G35910

Removes the phosphate from trehalose 6-phosphate to produce free trehalose

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0006485
Physical Location & Seq
Reverse (-)
13362962 .. 13365172
2211 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G35910.1

Sequence Viewer

Length: 1110 bp
ATGACAAACCATAATGCCTTAATCTCTGATGCTAAAGGCAGCATCGGAGTTGCGGTTAGAGTTCCAAACCAATCTCTGTTTTCTCCCGGAGGTGGCCGATACATCAGCATTCCCCGGAAGAAACTCGTGCAGAAGCTAGAGGCCGACCCGAGTCAAACCCGTATCCACACTTGGATCGAAGCCATGAGGGCTTCTTCCCCAACCCGTACCCGACCGGGGAACATATCTCCCCTCCCGGAGTCCGATGAGGAGGATGAATACTCTTCTTGGATGGCTCAACACCCGTCAGCTTTAACCATGTTTGAAGAGATAGCTGAAGCTTCAAAAGGGAAACAAATCGTGATGTTTCTCGACTATGACGGTACATTATCCCCCATTGTTGAAAACCCTGATCGAGCTTACATGTCTGAAGAGATGAGAGAGGCAGTGAAAGGCGTGGCTAGATATTTCCCGACCGCGATTGTCACTGGAAGATGCCGTGATAAGGTTCGTAGATTTGTGAAACTTCCCGGACTTTACTATGCAGGTAGCCATGGAATGGACATCAAAGGACCTTCCAAAAGAAACAAACATAACAAGAACAATAAAGGAGTTCTTTTCCAAGCGGCGAATGAGTTTTTGCCTATGATTGACAAGGTCTCTAAGTGTCTAGTAGAGAAAATGAGAGACATAGAAGGAGCAAACGTCGAGAACAACAAGTTTTGTGTCTCCGTACATTACCGTTGTGTTGATCAAAAGGACTGGGGATTGGTAGCGGAACACGTGACATCGATATTGAGTGAGTATCCGAAACTGAGGTTGACACAAGGAAGAAAAGTCTTAGAGATTCGACCAACCATCAAATGGGATAAAGGCAAAGCTCTCGAGTTCTTGCTCGAATCCTTAGGATTCGCTAACTCTAACGATGTTTTGCCCATCTATATAGGAGATGATCGTACGGACGAGGATGCTTTCAAGGTTTTGAGAAACAAAGGACAAGGCTTTGGTATACTTGTGTCCAAAATTCCAAAGGAAACGAGTGCTACATATTCTCTACAAGAACCTTCCGAGGTAGGAGAGTTTTTGCAGCGACTCGTGGAATGGAAACAAATGTCACTAAGAGGAAGATAG

Protein Analysis

369

Amino Acids

41.6

Weight (kDa)

9.03

Isoelectric Point (pI)

44.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Trehalose_PPase PF02358 116 - 349 7.4e-74 Trehalose-phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 515
AccB7I CCANNNNNTGG 2 cut(s) 538, 843
AccI GTMKAC 1 cut(s) 988
AccII CGCG 1 cut(s) 458
AciI CCGC 4 cut(s) 53, 456, 605, 755
AclWI GGATC 1 cut(s) 182
AcoI YGGCCR 1 cut(s) 94
AcsI RAATTY 1 cut(s) 1002
AcuI CTGAAG 2 cut(s) 336, 429
AcvI CACGTG 1 cut(s) 763
AfaI GTAC 4 cut(s) 208, 364, 714, 937
AfiI CCNNNNNNNGG 5 cut(s) 92, 216, 484, 538, 843
AflIII ACRYGT 2 cut(s) 402, 760
AgsI TTSAA 4 cut(s) 305, 324, 383, 955
AluBI AGCT 6 cut(s) 136, 290, 314, 320, 398, 860
AluI AGCT 6 cut(s) 136, 290, 314, 320, 398, 860
Alw26I GTCTC 3 cut(s) 643, 660, 712
AlwI GGATC 1 cut(s) 182
Ama87I CYCGRG 2 cut(s) 148, 863
AoxI GGCC 2 cut(s) 94, 141
ApeKI GCWGC 2 cut(s) 39, 1066
ApoI RAATTY 1 cut(s) 1002
AspS9I GGNCC 1 cut(s) 551
AsuC2I CCSGG 5 cut(s) 87, 115, 216, 236, 510
AvaI CYCGRG 2 cut(s) 148, 863
AvaII GGWCC 1 cut(s) 551
AxyI CCTNAGG 1 cut(s) 883
BauI CACGAG 2 cut(s) 125, 1073
BbrPI CACGTG 1 cut(s) 763
BbvI GCAGC 2 cut(s) 51, 1078
BccI CCATC 3 cut(s) 265, 845, 923
BceAI ACGGC 1 cut(s) 462
BcgI CGANNNNNNTGC 2 cut(s) 844, 878
BciVI GTATCC 2 cut(s) 173, 795
BclI TGATCA 1 cut(s) 730
BcnI CCSGG 5 cut(s) 87, 115, 216, 236, 510
BcoDI GTCTC 3 cut(s) 643, 660, 712
BfaI CTAG 3 cut(s) 137, 441, 650
BfuAI ACCTGC 1 cut(s) 515
BfuI GTATCC 2 cut(s) 173, 795
BglI GCCNNNNNGGC 1 cut(s) 188
BisI GCNGC 3 cut(s) 40, 606, 1067
BlsI GCNGC 3 cut(s) 41, 607, 1068
Bme1390I CCNGG 5 cut(s) 87, 115, 216, 236, 510
Bme18I GGWCC 1 cut(s) 551
BmeT110I CYCGRG 2 cut(s) 148, 863
BmgT120I GGNCC 1 cut(s) 551
BmrFI CCNGG 5 cut(s) 87, 115, 216, 236, 510
BmrI ACTGGG 1 cut(s) 751
BmsI GCATC 4 cut(s) 19, 51, 464, 937
BmuI ACTGGG 1 cut(s) 751
BoxI GACNNNNGTC 1 cut(s) 150
BpuMI CCSGG 5 cut(s) 87, 115, 216, 236, 510
Bsa29I ATCGAT 1 cut(s) 770
BsaAI YACGTR 1 cut(s) 763
BsaI GGTCTC 1 cut(s) 643
BsaJI CCNNGG 4 cut(s) 113, 215, 532, 1047
BsaXI ACNNNNNCTCC 4 cut(s) 39, 69, 669, 699
Bsc4I CCNNNNNNNGG 5 cut(s) 92, 216, 484, 538, 843
Bse1I ACTGG 2 cut(s) 472, 746
Bse21I CCTNAGG 1 cut(s) 883
BseCI ATCGAT 1 cut(s) 770
BseDI CCNNGG 4 cut(s) 113, 215, 532, 1047
BseGI GGATG 3 cut(s) 259, 276, 952
BseLI CCNNNNNNNGG 5 cut(s) 92, 216, 484, 538, 843
BseMII CTCAG 1 cut(s) 785
BseNI ACTGG 2 cut(s) 472, 746
BseRI GAGGAG 1 cut(s) 263
BseXI GCAGC 2 cut(s) 51, 1078
BsgI GTGCAG 1 cut(s) 149
Bsh1236I CGCG 1 cut(s) 458
Bsh1285I CGRYCG 2 cut(s) 215, 456
BshFI GGCC 2 cut(s) 96, 143
BshVI ATCGAT 1 cut(s) 770
BsiEI CGRYCG 2 cut(s) 215, 456
BsiHKCI CYCGRG 2 cut(s) 148, 863
BsiSI CCGG 5 cut(s) 87, 115, 215, 236, 510
BsiWI CGTACG 1 cut(s) 935
BslI CCNNNNNNNGG 5 cut(s) 92, 216, 484, 538, 843
BsmAI GTCTC 3 cut(s) 643, 660, 712
BsmI GAATGC 1 cut(s) 108
BsnI GGCC 2 cut(s) 96, 143
Bso31I GGTCTC 1 cut(s) 643
BsoBI CYCGRG 2 cut(s) 148, 863
Bsp143I GATC 4 cut(s) 174, 391, 730, 931
Bsp19I CCATGG 1 cut(s) 532
BspACI CCGC 4 cut(s) 53, 456, 605, 755
BspANI GGCC 2 cut(s) 96, 143
BspCNI CTCAG 1 cut(s) 786
BspDI ATCGAT 1 cut(s) 770
BspFNI CGCG 1 cut(s) 458
BspMI ACCTGC 1 cut(s) 515
BspPI GGATC 1 cut(s) 182
BspTNI GGTCTC 1 cut(s) 643
BsrI ACTGG 2 cut(s) 472, 746
BssECI CCNNGG 4 cut(s) 113, 215, 532, 1047
BssMI GATC 4 cut(s) 174, 391, 730, 931
BssNAI GTATAC 1 cut(s) 989
BssSI CACGAG 2 cut(s) 125, 1073
BssT1I CCWWGG 1 cut(s) 532
Bst1107I GTATAC 1 cut(s) 989
Bst2BI CACGAG 2 cut(s) 125, 1073
Bst4CI ACNGT 2 cut(s) 362, 722
Bst6I CTCTTC 3 cut(s) 268, 300, 405
BstBAI YACGTR 1 cut(s) 763
BstDEI CTNAG 5 cut(s) 642, 794, 820, 883, 1097
BstDSI CCRYGG 1 cut(s) 532
BstF5I GGATG 3 cut(s) 259, 276, 952
BstFNI CGCG 1 cut(s) 458
BstKTI GATC 4 cut(s) 177, 394, 733, 934
BstMAI GTCTC 3 cut(s) 643, 660, 712
BstMBI GATC 4 cut(s) 174, 391, 730, 931
BstMCI CGRYCG 2 cut(s) 215, 456
BstMWI GCNNNNNNNGC 1 cut(s) 188
BstNSI RCATGY 1 cut(s) 406
BstPAI GACNNNNGTC 1 cut(s) 150
BstSCI CCNGG 5 cut(s) 85, 113, 214, 234, 508
BstUI CGCG 1 cut(s) 458
BstV1I GCAGC 2 cut(s) 51, 1078
BstZ17I GTATAC 1 cut(s) 989
Bsu15I ATCGAT 1 cut(s) 770
Bsu36I CCTNAGG 1 cut(s) 883
BsuI GTATCC 2 cut(s) 173, 795
BsuRI GGCC 2 cut(s) 96, 143
BsuTUI ATCGAT 1 cut(s) 770
BtgI CCRYGG 1 cut(s) 532
BtsCI GGATG 3 cut(s) 259, 276, 952
BtsI GCAGTG 1 cut(s) 432
BtsIMutI CAGTG 2 cut(s) 432, 465
BveI ACCTGC 1 cut(s) 515
Cfr13I GGNCC 1 cut(s) 551
ClaI ATCGAT 1 cut(s) 770
Csp6I GTAC 4 cut(s) 207, 363, 713, 936
CviAII CATG 4 cut(s) 184, 298, 403, 533
CviQI GTAC 4 cut(s) 207, 363, 713, 936
DdeI CTNAG 5 cut(s) 642, 794, 820, 883, 1097
DpnI GATC 4 cut(s) 176, 393, 732, 933
DpnII GATC 4 cut(s) 174, 391, 730, 931
EaeI YGGCCR 1 cut(s) 94
Eam1104I CTCTTC 3 cut(s) 268, 300, 405
EarI CTCTTC 3 cut(s) 268, 300, 405
Eco130I CCWWGG 1 cut(s) 532
Eco31I GGTCTC 1 cut(s) 643
Eco47I GGWCC 1 cut(s) 551
Eco57I CTGAAG 2 cut(s) 336, 429
Eco72I CACGTG 1 cut(s) 763
Eco81I CCTNAGG 1 cut(s) 883
Eco88I CYCGRG 2 cut(s) 148, 863
EcoO109I RGGNCCY 1 cut(s) 551
EcoT14I CCWWGG 1 cut(s) 532
ErhI CCWWGG 1 cut(s) 532
FaeI CATG 4 cut(s) 187, 301, 406, 536
FalI AAGNNNNNCTT 2 cut(s) 579, 611
FatI CATG 4 cut(s) 183, 297, 402, 532
FbaI TGATCA 1 cut(s) 730
FblI GTMKAC 1 cut(s) 988
Fnu4HI GCNGC 3 cut(s) 40, 606, 1067
FokI GGATG 3 cut(s) 266, 283, 959
Fsp4HI GCNGC 3 cut(s) 40, 606, 1067
FspBI CTAG 3 cut(s) 137, 441, 650
GluI GCNGC 3 cut(s) 40, 606, 1067
HaeIII GGCC 2 cut(s) 96, 143
HapII CCGG 5 cut(s) 87, 115, 215, 236, 510
Hin1II CATG 4 cut(s) 187, 301, 406, 536
HincII GTYRAC 1 cut(s) 801
HindII GTYRAC 1 cut(s) 801
HindIII AAGCTT 1 cut(s) 318
HinfI GANTC 6 cut(s) 151, 239, 826, 878, 888, 1071
HpaII CCGG 5 cut(s) 87, 115, 215, 236, 510
Hpy166II GTNNAC 2 cut(s) 801, 989
Hpy188I TCNGA 6 cut(s) 28, 47, 244, 409, 789, 1048
Hpy188III TCNNGA 5 cut(s) 340, 350, 451, 688, 863
Hpy8I GTNNAC 2 cut(s) 801, 989
Hpy99I CGWCG 1 cut(s) 689
HpyAV CCTTC 3 cut(s) 564, 668, 1053
HpyCH4III ACNGT 2 cut(s) 362, 722
HpyCH4IV ACGT 2 cut(s) 684, 762
HpyCH4V TGCA 3 cut(s) 130, 524, 1066
HpyF10VI GCNNNNNNNGC 1 cut(s) 188
HpyF3I CTNAG 5 cut(s) 642, 794, 820, 883, 1097
HpySE526I ACGT 2 cut(s) 684, 762
Hsp92II CATG 4 cut(s) 187, 301, 406, 536
Ksp22I TGATCA 1 cut(s) 730
Kzo9I GATC 4 cut(s) 174, 391, 730, 931
LmnI GCTCC 1 cut(s) 677
LpnPI CCDG 9 cut(s) 100, 128, 228, 249, 402, 453, 510, 523, 727
Lsp1109I GCAGC 2 cut(s) 51, 1078
LweI GCATC 4 cut(s) 19, 51, 464, 937
MaeI CTAG 3 cut(s) 137, 441, 650
MaeII ACGT 2 cut(s) 684, 762
MaeIII GTNAC 3 cut(s) 463, 763, 1092
MalI GATC 4 cut(s) 176, 393, 732, 933
MboI GATC 4 cut(s) 174, 391, 730, 931
MboII GAAGA 7 cut(s) 130, 186, 255, 317, 422, 483, 822
MluCI AATT 1 cut(s) 1002
MlyI GAGTC 3 cut(s) 160, 248, 1065
MseI TTAA 2 cut(s) 20, 293
MspI CCGG 5 cut(s) 87, 115, 215, 236, 510
MspR9I CCNGG 5 cut(s) 87, 115, 216, 236, 510
Mva1269I GAATGC 1 cut(s) 108
MvnI CGCG 1 cut(s) 458
MwoI GCNNNNNNNGC 1 cut(s) 188
NciI CCSGG 5 cut(s) 87, 115, 216, 236, 510
NcoI CCATGG 1 cut(s) 532
NdeII GATC 4 cut(s) 174, 391, 730, 931
NlaIII CATG 4 cut(s) 187, 301, 406, 536
NmuCI GTSAC 3 cut(s) 463, 763, 1092
NspI RCATGY 1 cut(s) 406
PaeR7I CTCGAG 1 cut(s) 863
PciI ACATGT 1 cut(s) 402
PctI GAATGC 1 cut(s) 108
PfeI GAWTC 3 cut(s) 826, 878, 888
Pfl23II CGTACG 1 cut(s) 935
PflFI GACNNNGTC 1 cut(s) 635
PflMI CCANNNNNTGG 2 cut(s) 538, 843
PfoI TCCNGGA 3 cut(s) 85, 234, 508
PkrI GCNGC 3 cut(s) 41, 607, 1068
PleI GAGTC 3 cut(s) 159, 247, 1065
PmaCI CACGTG 1 cut(s) 763
PmlI CACGTG 1 cut(s) 763
PpsI GAGTC 3 cut(s) 159, 247, 1065
Ppu21I YACGTR 1 cut(s) 763
PpuMI RGGWCCY 1 cut(s) 551
PscI ACATGT 1 cut(s) 402
PshAI GACNNNNGTC 1 cut(s) 150
Psp5II RGGWCCY 1 cut(s) 551
PspCI CACGTG 1 cut(s) 763
PspLI CGTACG 1 cut(s) 935
PspPI GGNCC 1 cut(s) 551
PspPPI RGGWCCY 1 cut(s) 551
PsyI GACNNNGTC 1 cut(s) 635
RsaI GTAC 4 cut(s) 208, 364, 714, 937
RsaNI GTAC 4 cut(s) 207, 363, 713, 936
SaqAI TTAA 2 cut(s) 20, 293
SatI GCNGC 3 cut(s) 40, 606, 1067
Sau3AI GATC 4 cut(s) 174, 391, 730, 931
Sau96I GGNCC 1 cut(s) 551
SchI GAGTC 3 cut(s) 160, 248, 1065
ScrFI CCNGG 5 cut(s) 87, 115, 216, 236, 510
SfaNI GCATC 4 cut(s) 19, 51, 464, 937
Sfr274I CTCGAG 1 cut(s) 863
SinI GGWCC 1 cut(s) 551
SlaI CTCGAG 1 cut(s) 863
SmlI CTYRAG 1 cut(s) 863
SmoI CTYRAG 1 cut(s) 863
Sse9I AATT 1 cut(s) 1002
SsiI CCGC 4 cut(s) 53, 456, 605, 755
SspMI CTAG 3 cut(s) 137, 441, 650
StyD4I CCNGG 5 cut(s) 85, 113, 214, 234, 508
StyI CCWWGG 1 cut(s) 532
TaaI ACNGT 2 cut(s) 362, 722
TaiI ACGT 2 cut(s) 687, 765
TaqI TCGA 8 cut(s) 177, 351, 394, 687, 770, 829, 864, 876
TasI AATT 1 cut(s) 1002
TauI GCSGC 1 cut(s) 608
TfiI GAWTC 3 cut(s) 826, 878, 888
Tru1I TTAA 2 cut(s) 20, 293
Tru9I TTAA 2 cut(s) 20, 293
TscAI CASTG 2 cut(s) 432, 472
TseFI GTSAC 3 cut(s) 463, 763, 1092
TseI GCWGC 2 cut(s) 39, 1066
Tsp45I GTSAC 3 cut(s) 463, 763, 1092
TspDTI ATGAA 1 cut(s) 270
TspGWI ACGGA 2 cut(s) 700, 953
TspRI CASTG 2 cut(s) 432, 472
Tth111I GACNNNGTC 1 cut(s) 635
Van91I CCANNNNNTGG 2 cut(s) 538, 843
VpaK11BI GGWCC 1 cut(s) 551
XapI RAATTY 1 cut(s) 1002
XceI RCATGY 1 cut(s) 406
XcmI CCANNNNNNNNNTGG 1 cut(s) 840
XhoI CTCGAG 1 cut(s) 863
XmiI GTMKAC 1 cut(s) 988
XspI CTAG 3 cut(s) 137, 441, 650
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.