FvH4_3g04100

Removes the phosphate from trehalose 6-phosphate to produce free trehalose

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0006485
Physical Location & Seq
Reverse (-)
2288504 .. 2292230
3727 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g04100.t1

Sequence Viewer

Length: 1230 bp
ATGAACACTTTGAGGTTTTGCTTATTTTGCTTTGTGCTTGTTTTTGATATCAAAGGCGTGGCTATGGCTATGGCTGTTGCTGCCATGAATTCGAAGAACGACCGGTCTATGTTCTCTTCATCGGTGGCGAGTTCCGTGACTGAATCAGGAGGAGTACTTGGAGGGTGTAATTTCACCATTCCCAGGAGTACTATTAAGCTCTTGAAGGTTGAGACTACTGCAGAAGTTGGTGGTGATGGTGGTGGAGCTGCCCGAGTCAGTGCTTGGGTAGACTCAATGAGAGCTTCTTCTCCGCCGCGTGTTAGATCCACCATTACTTCCCCCTCCACTCTTTCTCAAACTGGGGATGAACAAAACTCTTGGAACCTTAGTCACCCATCAGCTATAAACATGTTTGAGGAGATCACAAATGCTTCAAAGGGGAAGCAGATTGTGATGTTTCTTGACTACGATGGAACCCTTTCTCCAATTGTTGAAGACCCTGACAAAGCCTTCATGTCCAATGAGATGAGAGAAGCTGTGAGAGCAGTTGCTAAATATTTTCCTACAGCCATAGTTAGTGGGCGGAGTCGACACAAGGTTTATAGCTTTGTCAAACTAGCAGAGCTTTACTATGCAGGAAGCCATGGGATGGACATTAAAGGACCATCCAAGAGAAACAAGTACAAGAATGCTATTAATCAAGCAGTTCTGTACCAACCAGCCAGTGCCTTTGTACCCATGATTGCTGAGGTATGCAAAATACTAGTGAAGAAAACCAAATCCATCTCAGGAGCTAAGGTGGAAAATAATAAGTTCTGTTTATCCGTACACTTCCGTTGTGTTGAGGAGAAGAGTTGGGATGCTCTGGCAAAGCAAGTAAGCTTAGTGCTCGATGAGTATCCAGAGCTGAAGTTGACTCAAGGGAGAAAGGTCTTAGAGATCAAGCCAGATATCAAATGGGACAAGGGCAAAGCACTTGAATTCTTGCTAAAGTCATTAGGATACGCCAATAACTCAAGTGAAGTATTACCGGTCTATATTGGAGATGATAGAACAGATGAGGATGCTTTCAAGGTTTTACGTGACAGAGGACAAGGACAAGGAATTGGAATTCTTGTTTCTAAAGTTCCGAAAGAAACAAAAGCTTCCTATACCTTGCAAGAACCAGCAGAGGTCAAGGACTTTTTACAACGTCTAGTGAAGTGGAAAAGAATGACGTCGCTGCAGTCACAAAGAATGGAAGCCTAA

Protein Analysis

410

Amino Acids

45.16

Weight (kDa)

9.25

Isoelectric Point (pI)

37.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Trehalose_PPase PF02358 147 - 383 3.1e-72 Trehalose-phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1202
AccB7I CCANNNNNTGG 1 cut(s) 631
AccI GTMKAC 2 cut(s) 270, 571
AccII CGCG 1 cut(s) 298
AciI CCGC 3 cut(s) 293, 296, 565
AclWI GGATC 1 cut(s) 300
AcsI RAATTY 3 cut(s) 88, 962, 1092
AcuI CTGAAG 1 cut(s) 911
AcyI GRCGYC 1 cut(s) 1199
AfaI GTAC 6 cut(s) 156, 190, 665, 695, 717, 810
AfiI CCNNNNNNNGG 2 cut(s) 183, 631
AflIII ACRYGT 1 cut(s) 390
AgeI ACCGGT 2 cut(s) 102, 1012
AgsI TTSAA 5 cut(s) 205, 417, 476, 962, 1054
AhlI ACTAGT 1 cut(s) 745
AjnI CCWGG 1 cut(s) 182
AloI GAACNNNNNNTCC 2 cut(s) 448, 480
Alw21I GWGCWC 1 cut(s) 873
Alw26I GTCTC 1 cut(s) 206
AlwI GGATC 1 cut(s) 300
Ama87I CYCGRG 1 cut(s) 252
ApeKI GCWGC 3 cut(s) 80, 248, 1204
ApoI RAATTY 3 cut(s) 88, 962, 1092
AseI ATTAAT 1 cut(s) 678
AsiGI ACCGGT 2 cut(s) 102, 1012
Asp700I GAANNNNTTC 1 cut(s) 460
AspS9I GGNCC 1 cut(s) 644
AsuHPI GGTGA 3 cut(s) 166, 245, 365
AsuII TTCGAA 1 cut(s) 92
AvaI CYCGRG 1 cut(s) 252
AvaII GGWCC 1 cut(s) 644
BbsI GAAGAC 1 cut(s) 483
Bbv12I GWGCWC 1 cut(s) 873
BbvCI CCTCAGC 1 cut(s) 729
BbvI GCAGC 3 cut(s) 67, 235, 1191
BccI CCATC 6 cut(s) 230, 385, 446, 625, 655, 773
BciT130I CCWGG 1 cut(s) 184
BciVI GTATCC 2 cut(s) 891, 977
BcoDI GTCTC 1 cut(s) 206
BcuI ACTAGT 1 cut(s) 745
BfaI CTAG 3 cut(s) 599, 746, 1178
BfmI CTRYAG 3 cut(s) 219, 546, 1205
BfuI GTATCC 2 cut(s) 891, 977
BisI GCNGC 4 cut(s) 81, 249, 296, 1205
BlsI GCNGC 4 cut(s) 82, 250, 297, 1206
BmcAI AGTACT 2 cut(s) 156, 190
Bme1390I CCNGG 1 cut(s) 184
Bme18I GGWCC 1 cut(s) 644
BmeT110I CYCGRG 1 cut(s) 252
BmgT120I GGNCC 1 cut(s) 644
BmiI GGNNCC 2 cut(s) 365, 457
BmrFI CCNGG 1 cut(s) 184
BmrI ACTGGG 1 cut(s) 351
BmsI GCATC 2 cut(s) 832, 1036
BmuI ACTGGG 1 cut(s) 351
BpiI GAAGAC 1 cut(s) 483
Bpu10I CCTNAGC 2 cut(s) 729, 777
Bpu14I TTCGAA 1 cut(s) 92
BpuEI CTTGAG 2 cut(s) 885, 982
BsaAI YACGTR 1 cut(s) 1064
BsaBI GATNNNNATC 1 cut(s) 879
BsaHI GRCGYC 1 cut(s) 1199
BsaJI CCNNGG 2 cut(s) 182, 625
BsaWI WCCGGW 2 cut(s) 102, 1012
BsaXI ACNNNNNCTCC 4 cut(s) 448, 478, 765, 795
Bsc4I CCNNNNNNNGG 2 cut(s) 183, 631
Bse118I RCCGGY 2 cut(s) 102, 1012
Bse1I ACTGG 2 cut(s) 346, 705
Bse8I GATNNNNATC 1 cut(s) 879
BseBI CCWGG 1 cut(s) 184
BseDI CCNNGG 2 cut(s) 182, 625
BseGI GGATG 5 cut(s) 352, 636, 647, 847, 1051
BseJI GATNNNNATC 1 cut(s) 879
BseLI CCNNNNNNNGG 2 cut(s) 183, 631
BseMII CTCAG 2 cut(s) 720, 783
BseNI ACTGG 2 cut(s) 346, 705
BseRI GAGGAG 3 cut(s) 165, 413, 842
BseXI GCAGC 3 cut(s) 67, 235, 1191
Bsh1236I CGCG 1 cut(s) 298
Bsh1285I CGRYCG 1 cut(s) 103
BshTI ACCGGT 2 cut(s) 102, 1012
BsiEI CGRYCG 1 cut(s) 103
BsiHKAI GWGCWC 1 cut(s) 873
BsiHKCI CYCGRG 1 cut(s) 252
BsiSI CCGG 2 cut(s) 103, 1013
BslFI GGGAC 1 cut(s) 956
BslI CCNNNNNNNGG 2 cut(s) 183, 631
BsmAI GTCTC 1 cut(s) 206
BsmFI GGGAC 1 cut(s) 956
BsmI GAATGC 1 cut(s) 676
BsoBI CYCGRG 1 cut(s) 252
Bsp119I TTCGAA 1 cut(s) 92
Bsp1286I GDGCHC 1 cut(s) 873
Bsp143I GATC 3 cut(s) 305, 402, 921
Bsp19I CCATGG 1 cut(s) 625
BspACI CCGC 3 cut(s) 293, 296, 565
BspCNI CTCAG 2 cut(s) 721, 782
BspFNI CGCG 1 cut(s) 298
BspLI GGNNCC 2 cut(s) 365, 457
BspMAI CTGCAG 2 cut(s) 223, 1209
BspPI GGATC 1 cut(s) 300
BspT104I TTCGAA 1 cut(s) 92
BsrFI RCCGGY 2 cut(s) 102, 1012
BsrI ACTGG 2 cut(s) 346, 705
BssAI RCCGGY 2 cut(s) 102, 1012
BssECI CCNNGG 2 cut(s) 182, 625
BssMI GATC 3 cut(s) 305, 402, 921
BssNI GRCGYC 1 cut(s) 1199
BssT1I CCWWGG 1 cut(s) 625
Bst2UI CCWGG 1 cut(s) 184
Bst6I CTCTTC 2 cut(s) 121, 827
BstACI GRCGYC 1 cut(s) 1199
BstBAI YACGTR 1 cut(s) 1064
BstBI TTCGAA 1 cut(s) 92
BstDEI CTNAG 6 cut(s) 368, 729, 769, 777, 865, 916
BstDSI CCRYGG 1 cut(s) 625
BstF5I GGATG 5 cut(s) 352, 636, 647, 847, 1051
BstFNI CGCG 1 cut(s) 298
BstKTI GATC 3 cut(s) 308, 405, 924
BstMAI GTCTC 1 cut(s) 206
BstMBI GATC 3 cut(s) 305, 402, 921
BstMCI CGRYCG 1 cut(s) 103
BstMWI GCNNNNNNNGC 3 cut(s) 27, 80, 524
BstNI CCWGG 1 cut(s) 184
BstNSI RCATGY 1 cut(s) 394
BstSCI CCNGG 1 cut(s) 182
BstSFI CTRYAG 3 cut(s) 219, 546, 1205
BstUI CGCG 1 cut(s) 298
BstV1I GCAGC 3 cut(s) 67, 235, 1191
BstV2I GAAGAC 1 cut(s) 483
BstX2I RGATCY 1 cut(s) 305
BstYI RGATCY 1 cut(s) 305
BsuI GTATCC 2 cut(s) 891, 977
BtgI CCRYGG 1 cut(s) 625
BtsCI GGATG 5 cut(s) 352, 636, 647, 847, 1051
BtsIMutI CAGTG 2 cut(s) 265, 712
Cfr10I RCCGGY 2 cut(s) 102, 1012
Cfr13I GGNCC 1 cut(s) 644
Csp6I GTAC 6 cut(s) 155, 189, 664, 694, 716, 809
CspAI ACCGGT 2 cut(s) 102, 1012
CviAII CATG 5 cut(s) 85, 391, 496, 626, 721
CviQI GTAC 6 cut(s) 155, 189, 664, 694, 716, 809
DdeI CTNAG 6 cut(s) 368, 729, 769, 777, 865, 916
DpnI GATC 3 cut(s) 307, 404, 923
DpnII GATC 3 cut(s) 305, 402, 921
Eam1104I CTCTTC 2 cut(s) 121, 827
EarI CTCTTC 2 cut(s) 121, 827
EciI GGCGGA 2 cut(s) 282, 580
Eco130I CCWWGG 1 cut(s) 625
Eco32I GATATC 2 cut(s) 49, 934
Eco47I GGWCC 1 cut(s) 644
Eco57I CTGAAG 1 cut(s) 911
Eco88I CYCGRG 1 cut(s) 252
EcoRI GAATTC 3 cut(s) 88, 962, 1092
EcoRII CCWGG 1 cut(s) 182
EcoRV GATATC 2 cut(s) 49, 934
EcoT14I CCWWGG 1 cut(s) 625
ErhI CCWWGG 1 cut(s) 625
FaeI CATG 5 cut(s) 88, 394, 499, 629, 724
FaqI GGGAC 1 cut(s) 956
FatI CATG 5 cut(s) 84, 390, 495, 625, 720
FblI GTMKAC 2 cut(s) 270, 571
Fnu4HI GCNGC 4 cut(s) 81, 249, 296, 1205
FokI GGATG 5 cut(s) 359, 634, 643, 854, 1058
Fsp4HI GCNGC 4 cut(s) 81, 249, 296, 1205
FspBI CTAG 3 cut(s) 599, 746, 1178
GluI GCNGC 4 cut(s) 81, 249, 296, 1205
HapII CCGG 2 cut(s) 103, 1013
Hin1I GRCGYC 1 cut(s) 1199
Hin1II CATG 5 cut(s) 88, 394, 499, 629, 724
HincII GTYRAC 2 cut(s) 572, 897
HindII GTYRAC 2 cut(s) 572, 897
HindIII AAGCTT 2 cut(s) 862, 1125
HinfI GANTC 5 cut(s) 143, 255, 272, 568, 898
HpaII CCGG 2 cut(s) 103, 1013
HphI GGTGA 3 cut(s) 166, 245, 365
Hpy166II GTNNAC 4 cut(s) 271, 572, 811, 897
Hpy188I TCNGA 1 cut(s) 1113
Hpy188III TCNNGA 5 cut(s) 147, 202, 443, 771, 884
Hpy8I GTNNAC 4 cut(s) 271, 572, 811, 897
Hpy99I CGWCG 1 cut(s) 1204
HpyAV CCTTC 2 cut(s) 199, 502
HpyCH4IV ACGT 3 cut(s) 1063, 1174, 1199
HpyCH4V TGCA 5 cut(s) 221, 617, 738, 1141, 1207
HpyF10VI GCNNNNNNNGC 3 cut(s) 27, 80, 524
HpyF3I CTNAG 6 cut(s) 368, 729, 769, 777, 865, 916
HpySE526I ACGT 3 cut(s) 1063, 1174, 1199
Hsp92I GRCGYC 1 cut(s) 1199
Hsp92II CATG 5 cut(s) 88, 394, 499, 629, 724
Kzo9I GATC 3 cut(s) 305, 402, 921
LmnI GCTCC 2 cut(s) 245, 773
Lsp1109I GCAGC 3 cut(s) 67, 235, 1191
LweI GCATC 2 cut(s) 832, 1036
MaeI CTAG 3 cut(s) 599, 746, 1178
MaeII ACGT 3 cut(s) 1063, 1174, 1199
MaeIII GTNAC 4 cut(s) 136, 371, 1064, 1209
MalI GATC 3 cut(s) 307, 404, 923
MboI GATC 3 cut(s) 305, 402, 921
MboII GAAGA 6 cut(s) 106, 108, 279, 488, 763, 844
MfeI CAATTG 1 cut(s) 468
MflI RGATCY 1 cut(s) 305
MhlI GDGCHC 1 cut(s) 873
MluCI AATT 6 cut(s) 88, 169, 468, 962, 1086, 1092
MlyI GAGTC 4 cut(s) 264, 266, 577, 892
MroXI GAANNNNTTC 1 cut(s) 460
MseI TTAA 3 cut(s) 195, 639, 678
MspI CCGG 2 cut(s) 103, 1013
MspR9I CCNGG 1 cut(s) 184
MunI CAATTG 1 cut(s) 468
Mva1269I GAATGC 1 cut(s) 676
MvaI CCWGG 1 cut(s) 184
MvnI CGCG 1 cut(s) 298
MwoI GCNNNNNNNGC 3 cut(s) 27, 80, 524
NcoI CCATGG 1 cut(s) 625
NdeII GATC 3 cut(s) 305, 402, 921
NlaIII CATG 5 cut(s) 88, 394, 499, 629, 724
NlaIV GGNNCC 2 cut(s) 365, 457
NmuCI GTSAC 4 cut(s) 136, 371, 1064, 1209
NspI RCATGY 1 cut(s) 394
NspV TTCGAA 1 cut(s) 92
PciI ACATGT 1 cut(s) 390
PctI GAATGC 1 cut(s) 676
PdmI GAANNNNTTC 1 cut(s) 460
PfeI GAWTC 1 cut(s) 143
PflMI CCANNNNNTGG 1 cut(s) 631
PinAI ACCGGT 2 cut(s) 102, 1012
PkrI GCNGC 4 cut(s) 82, 250, 297, 1206
PleI GAGTC 4 cut(s) 263, 266, 576, 892
PpsI GAGTC 4 cut(s) 263, 266, 576, 892
Ppu21I YACGTR 1 cut(s) 1064
PscI ACATGT 1 cut(s) 390
PshBI ATTAAT 1 cut(s) 678
Psp6I CCWGG 1 cut(s) 182
PspGI CCWGG 1 cut(s) 182
PspN4I GGNNCC 2 cut(s) 365, 457
PspPI GGNCC 1 cut(s) 644
PstI CTGCAG 2 cut(s) 223, 1209
PsuI RGATCY 1 cut(s) 305
RsaI GTAC 6 cut(s) 156, 190, 665, 695, 717, 810
RsaNI GTAC 6 cut(s) 155, 189, 664, 694, 716, 809
SalI GTCGAC 1 cut(s) 570
SaqAI TTAA 3 cut(s) 195, 639, 678
SatI GCNGC 4 cut(s) 81, 249, 296, 1205
Sau3AI GATC 3 cut(s) 305, 402, 921
Sau96I GGNCC 1 cut(s) 644
ScaI AGTACT 2 cut(s) 156, 190
SchI GAGTC 4 cut(s) 264, 266, 577, 892
ScrFI CCNGG 1 cut(s) 184
SduI GDGCHC 1 cut(s) 873
SfaNI GCATC 2 cut(s) 832, 1036
SfcI CTRYAG 3 cut(s) 219, 546, 1205
SfuI TTCGAA 1 cut(s) 92
SinI GGWCC 1 cut(s) 644
SmlI CTYRAG 2 cut(s) 900, 997
SmoI CTYRAG 2 cut(s) 900, 997
SpeI ACTAGT 1 cut(s) 745
Sse9I AATT 6 cut(s) 88, 169, 468, 962, 1086, 1092
SsiI CCGC 3 cut(s) 293, 296, 565
SspI AATATT 1 cut(s) 539
SspMI CTAG 3 cut(s) 599, 746, 1178
StyD4I CCNGG 1 cut(s) 182
StyI CCWWGG 1 cut(s) 625
TaiI ACGT 3 cut(s) 1066, 1177, 1202
TaqI TCGA 3 cut(s) 92, 571, 873
TasI AATT 6 cut(s) 88, 169, 468, 962, 1086, 1092
TatI WGTACW 3 cut(s) 154, 188, 663
TauI GCSGC 1 cut(s) 298
TfiI GAWTC 1 cut(s) 143
Tru1I TTAA 3 cut(s) 195, 639, 678
Tru9I TTAA 3 cut(s) 195, 639, 678
TscAI CASTG 2 cut(s) 265, 712
TseFI GTSAC 4 cut(s) 136, 371, 1064, 1209
TseI GCWGC 3 cut(s) 80, 248, 1204
Tsp45I GTSAC 4 cut(s) 136, 371, 1064, 1209
TspDTI ATGAA 5 cut(s) 17, 101, 108, 363, 484
TspGWI ACGGA 3 cut(s) 124, 796, 806
TspRI CASTG 2 cut(s) 265, 712
Van91I CCANNNNNTGG 1 cut(s) 631
VpaK11BI GGWCC 1 cut(s) 644
VspI ATTAAT 1 cut(s) 678
XapI RAATTY 3 cut(s) 88, 962, 1092
XceI RCATGY 1 cut(s) 394
XcmI CCANNNNNNNNNTGG 1 cut(s) 936
XmiI GTMKAC 2 cut(s) 270, 571
XmnI GAANNNNTTC 1 cut(s) 460
XspI CTAG 3 cut(s) 599, 746, 1178
ZraI GACGTC 1 cut(s) 1200
ZrmI AGTACT 2 cut(s) 156, 190
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.