Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0006485

Physical Location & Seq

Genomic Coordinates Forward (+)

22265888 .. 22268679

Gene Structure 2792 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1180400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1092 bp

ATGGCTATTACAGTCACGGTTGCTGCAAAAAAGGCTATGTTTTCACAGTCGGTTCCGAAACCGATGACTGAGGGAGGAGGAGGAGGTTGCTTCACCATTCCCATGAACATCAAGGTTTTGAAAGTTGACACAGAAGGCGGAGCAGCCAGAGTCAATGCTTGGGTTGACTCCTTGAGAGCATCTTCTCCAACTCGTGTCAAATCAGCCACTCCTCAGTCTCTTTCTGAAACCGTGGATTATAACTCTTGGACTATTCATCATCCATCAGCCATGAGCATGTTCGAGCAGATAACCAATGCTTCAAAGGGCAAGCAGATTGTAATGTTTCTTGACTATGATGGCACCCTCTCCCCCATTGTAGAAGACCCAGATAGTGCTTTCATGTCCAATGAGATGAGAAAAGCAGTGAGGGACGCTGCTAGATACTTTCCCACAGCAATAGTTAGCGGGAGGTGCAGAGACAAGGTTTATAGCTTTGTACAATTAGGAGAGCTTTATTATGCAGGCAGCCATGGGATGGACATCAGGGGACCATCCAAAAGTAGCAAATATAAGAATGATAATCAAGCGGTTCTCTTCCAACCAGCCAGTGAGTTTGTACCAATGATTGATGAGGTGTACAAAACTTTATTGGAGAAAACTAGATCCATCTCAGGATCTAGGGTAGAAAATAATAAATTTTGCTTATCTGTACACTTCCGTTGTGTTGATGAGAAGAGTTGGGCAGCTTTAGCAGAGCAAGTTAGACTTGTACTCAATGAGTACCCAAAGCTCAGATTGACTCAAGGGAGGAAGGTATTAGAGATCAGGCCAACAATCAAATGGGACAAAGGCAAAGCTCTTGAATTCTTGCTAGAGTCATTAGGATATGCGAACTCAAATGAAGTATTTCCAGTCTATATTGGAGATGATCGAACAGACGAGGATGCCTTCAAGGTGCTACGAGATAGAGGACAAGGATTTGGTATTCTAGTTTCTAAAGTTCCAAAAGAAACAAATGCTTCTTATACTTTACAAGAACCAGCGGAGGTTAAGGACTTTTTGCGGCGTTTGGTGAAATGGAAGAGATTGTCGAACCAACAAAGGAACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004805 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006793 GO:0006796 GO:0006950 GO:0006970 GO:0006979 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009628 GO:0009651 GO:0009987 GO:0015927 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0016798 GO:0019203 GO:0042578 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044424 GO:0044444 GO:0044464 GO:0050896

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 map00500 map01100

Pfam Domains

Protein Families

Trehalose_PPase

Protein Analysis

364

Amino Acids

40.73

Weight (kDa)

9.2

Isoelectric Point (pI)

34.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Trehalose_PPase	PF02358	109 - 341	2.4e-73	Trehalose-phosphatase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0006485)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G35910
fragaria_vesca	FvH4_3g04100 FvH4_3g04100 FvH4_3g04100 FvH4_3g04100 FvH4_5g37780 FvH4_5g37780 FvH4_5g37780 FvH4_5g37780
malus_domestica	MD08G1180400.v1.1 MD15G1365900.v1.1
prunus_persica	Prupe.1G513800_v2.0.a1
pyrus_communis	pycom08g15440 pycom15g32820
rosa_chinensis	RchiOBHm_Chr7g0239531
rosa_laevigata	RLG00000000747
rosa_multiflora	Rmu_sc0003379.1_g000024
rosa_roxburghii	Rroxscaffold_3G00220860
rosa_rugosa	Rorug07G0328400 Rorug07G0328400
rosa_samantha	Rh7AG482300 Rh7BG286700 Rh7BG455100 Rh7CG500000 Rh7DG468200
rosa_wichuraiana	Rw7G040390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	240
AccB1I	GGYRCC	1 cut(s)	341
AccB7I	CCANNNNNTGG	1 cut(s)	517
AciI	CCGC	5 cut(s)	138, 447, 569, 1025, 1045
AclWI	GGATC	2 cut(s)	639, 664
AcsI	RAATTY	2 cut(s)	677, 845
AfaI	GTAC	6 cut(s)	480, 600, 620, 693, 753, 764
AfiI	CCNNNNNNNGG	1 cut(s)	517
AgsI	TTSAA	4 cut(s)	121, 303, 845, 934
AluBI	AGCT	5 cut(s)	474, 493, 728, 772, 839
AluI	AGCT	5 cut(s)	474, 493, 728, 772, 839
Alw26I	GTCTC	2 cut(s)	222, 453
AlwI	GGATC	2 cut(s)	639, 664
AoxI	GGCC	1 cut(s)	809
ApeKI	GCWGC	5 cut(s)	23, 143, 416, 507, 725
ApoI	RAATTY	2 cut(s)	677, 845
Asp700I	GAANNNNTTC	1 cut(s)	888
AspS9I	GGNCC	1 cut(s)	530
AsuHPI	GGTGA	2 cut(s)	85, 1066
AvaII	GGWCC	1 cut(s)	530
BanI	GGYRCC	1 cut(s)	341
BauI	CACGAG	1 cut(s)	192
BbsI	GAAGAC	1 cut(s)	369
BbvI	GCAGC	5 cut(s)	10, 155, 403, 519, 737
BccI	CCATC	5 cut(s)	271, 332, 511, 541, 656
BcoDI	GTCTC	2 cut(s)	222, 453
BfaI	CTAG	6 cut(s)	420, 642, 660, 854, 971, 1090
BisI	GCNGC	6 cut(s)	24, 144, 417, 508, 726, 1046
BlsI	GCNGC	6 cut(s)	25, 145, 418, 509, 727, 1047
Bme18I	GGWCC	1 cut(s)	530
BmgT120I	GGNCC	1 cut(s)	530
BmiI	GGNNCC	3 cut(s)	54, 343, 531
BmsI	GCATC	2 cut(s)	188, 916
BpiI	GAAGAC	1 cut(s)	369
BpuEI	CTTGAG	2 cut(s)	193, 768
BsaBI	GATNNNNATC	1 cut(s)	521
BsaJI	CCNNGG	2 cut(s)	231, 511
Bsc4I	CCNNNNNNNGG	1 cut(s)	517
Bse1I	ACTGG	2 cut(s)	588, 893
Bse8I	GATNNNNATC	1 cut(s)	521
BseDI	CCNNGG	2 cut(s)	231, 511
BseGI	GGATG	4 cut(s)	259, 522, 533, 931
BseJI	GATNNNNATC	1 cut(s)	521
BseLI	CCNNNNNNNGG	1 cut(s)	517
BseMII	CTCAG	4 cut(s)	60, 227, 666, 787
BseNI	ACTGG	2 cut(s)	588, 893
BseRI	GAGGAG	4 cut(s)	90, 93, 96, 201
BseXI	GCAGC	5 cut(s)	10, 155, 403, 519, 737
BsgI	GTGCAG	1 cut(s)	475
BshFI	GGCC	1 cut(s)	811
BshNI	GGYRCC	1 cut(s)	341
BslFI	GGGAC	3 cut(s)	425, 543, 839
BslI	CCNNNNNNNGG	1 cut(s)	517
BsmAI	GTCTC	2 cut(s)	222, 453
BsmFI	GGGAC	3 cut(s)	425, 543, 839
BsnI	GGCC	1 cut(s)	811
Bsp1407I	TGTACA	3 cut(s)	478, 618, 691
Bsp143I	GATC	4 cut(s)	644, 656, 804, 910
Bsp19I	CCATGG	1 cut(s)	511
BspACI	CCGC	5 cut(s)	138, 447, 569, 1025, 1045
BspANI	GGCC	1 cut(s)	811
BspCNI	CTCAG	4 cut(s)	61, 226, 665, 786
BspLI	GGNNCC	3 cut(s)	54, 343, 531
BspPI	GGATC	2 cut(s)	639, 664
BspT107I	GGYRCC	1 cut(s)	341
BsrGI	TGTACA	3 cut(s)	478, 618, 691
BsrI	ACTGG	2 cut(s)	588, 893
BssECI	CCNNGG	2 cut(s)	231, 511
BssMI	GATC	4 cut(s)	644, 656, 804, 910
BssSI	CACGAG	1 cut(s)	192
BssT1I	CCWWGG	1 cut(s)	511
Bst2BI	CACGAG	1 cut(s)	192
Bst4CI	ACNGT	4 cut(s)	13, 19, 48, 232
Bst6I	CTCTTC	3 cut(s)	581, 710, 1058
BstAUI	TGTACA	3 cut(s)	478, 618, 691
BstC8I	GCNNGC	2 cut(s)	311, 505
BstDEI	CTNAG	4 cut(s)	69, 213, 652, 773
BstDSI	CCRYGG	2 cut(s)	231, 511
BstF5I	GGATG	4 cut(s)	259, 522, 533, 931
BstKTI	GATC	4 cut(s)	647, 659, 807, 913
BstMAI	GTCTC	2 cut(s)	222, 453
BstMBI	GATC	4 cut(s)	644, 656, 804, 910
BstMWI	GCNNNNNNNGC	3 cut(s)	32, 453, 731
BstNSI	RCATGY	1 cut(s)	280
BstV1I	GCAGC	5 cut(s)	10, 155, 403, 519, 737
BstV2I	GAAGAC	1 cut(s)	369
BstX2I	RGATCY	2 cut(s)	644, 656
BstYI	RGATCY	2 cut(s)	644, 656
BsuRI	GGCC	1 cut(s)	811
BtgI	CCRYGG	2 cut(s)	231, 511
BtsCI	GGATG	4 cut(s)	259, 522, 533, 931
BtsI	GCAGTG	1 cut(s)	411
BtsIMutI	CAGTG	2 cut(s)	411, 595
Cac8I	GCNNGC	2 cut(s)	311, 505
Cfr13I	GGNCC	1 cut(s)	530
CseI	GACGC	1 cut(s)	422
Csp6I	GTAC	6 cut(s)	479, 599, 619, 692, 752, 763
CviAII	CATG	5 cut(s)	103, 271, 277, 382, 512
CviQI	GTAC	6 cut(s)	479, 599, 619, 692, 752, 763
DdeI	CTNAG	4 cut(s)	69, 213, 652, 773
DpnI	GATC	4 cut(s)	646, 658, 806, 912
DpnII	GATC	4 cut(s)	644, 656, 804, 910
Eam1104I	CTCTTC	3 cut(s)	581, 710, 1058
EarI	CTCTTC	3 cut(s)	581, 710, 1058
EciI	GGCGGA	1 cut(s)	153
Eco130I	CCWWGG	1 cut(s)	511
Eco47I	GGWCC	1 cut(s)	530
EcoRI	GAATTC	1 cut(s)	845
EcoT14I	CCWWGG	1 cut(s)	511
ErhI	CCWWGG	1 cut(s)	511
FaeI	CATG	5 cut(s)	106, 274, 280, 385, 515
FalI	AAGNNNNNCTT	2 cut(s)	732, 764
FaqI	GGGAC	3 cut(s)	425, 543, 839
FatI	CATG	5 cut(s)	102, 270, 276, 381, 511
FauI	CCCGC	1 cut(s)	440
Fnu4HI	GCNGC	6 cut(s)	24, 144, 417, 508, 726, 1046
FokI	GGATG	4 cut(s)	246, 520, 529, 938
Fsp4HI	GCNGC	6 cut(s)	24, 144, 417, 508, 726, 1046
FspBI	CTAG	6 cut(s)	420, 642, 660, 854, 971, 1090
GluI	GCNGC	6 cut(s)	24, 144, 417, 508, 726, 1046
HaeIII	GGCC	1 cut(s)	811
HgaI	GACGC	1 cut(s)	422
Hin1II	CATG	5 cut(s)	106, 274, 280, 385, 515
HincII	GTYRAC	2 cut(s)	127, 166
HindII	GTYRAC	2 cut(s)	127, 166
HinfI	GANTC	4 cut(s)	150, 167, 781, 857
HphI	GGTGA	2 cut(s)	85, 1066
Hpy166II	GTNNAC	4 cut(s)	127, 166, 619, 694
Hpy188I	TCNGA	3 cut(s)	57, 226, 776
Hpy188III	TCNNGA	3 cut(s)	329, 654, 842
Hpy8I	GTNNAC	4 cut(s)	127, 166, 619, 694
HpyAV	CCTTC	3 cut(s)	128, 787, 940
HpyCH4III	ACNGT	4 cut(s)	13, 19, 48, 232
HpyCH4V	TGCA	3 cut(s)	26, 456, 503
HpyF10VI	GCNNNNNNNGC	3 cut(s)	32, 453, 731
HpyF3I	CTNAG	4 cut(s)	69, 213, 652, 773
Hsp92II	CATG	5 cut(s)	106, 274, 280, 385, 515
Kzo9I	GATC	4 cut(s)	644, 656, 804, 910
LmnI	GCTCC	1 cut(s)	140
Lsp1109I	GCAGC	5 cut(s)	10, 155, 403, 519, 737
LweI	GCATC	2 cut(s)	188, 916
MaeI	CTAG	6 cut(s)	420, 642, 660, 854, 971, 1090
MaeIII	GTNAC	1 cut(s)	13
MalI	GATC	4 cut(s)	646, 658, 806, 912
MboI	GATC	4 cut(s)	644, 656, 804, 910
MboII	GAAGA	5 cut(s)	174, 374, 568, 727, 1075
MflI	RGATCY	2 cut(s)	644, 656
MluCI	AATT	3 cut(s)	482, 677, 845
MlyI	GAGTC	4 cut(s)	159, 161, 775, 866
MmeI	TCCRAC	2 cut(s)	212, 604
MroXI	GAANNNNTTC	1 cut(s)	888
MseI	TTAA	1 cut(s)	1032
MslI	CAYNNNNRTG	2 cut(s)	101, 275
MspA1I	CMGCKG	1 cut(s)	1025
MwoI	GCNNNNNNNGC	3 cut(s)	32, 453, 731
NcoI	CCATGG	1 cut(s)	511
NdeII	GATC	4 cut(s)	644, 656, 804, 910
NlaIII	CATG	5 cut(s)	106, 274, 280, 385, 515
NlaIV	GGNNCC	3 cut(s)	54, 343, 531
NmuCI	GTSAC	1 cut(s)	13
NspI	RCATGY	1 cut(s)	280
PdmI	GAANNNNTTC	1 cut(s)	888
PflMI	CCANNNNNTGG	1 cut(s)	517
PkrI	GCNGC	6 cut(s)	25, 145, 418, 509, 727, 1047
PleI	GAGTC	4 cut(s)	158, 161, 775, 865
PpsI	GAGTC	4 cut(s)	158, 161, 775, 865
PsiI	TTATAA	1 cut(s)	240
PspN4I	GGNNCC	3 cut(s)	54, 343, 531
PspPI	GGNCC	1 cut(s)	530
PsuI	RGATCY	2 cut(s)	644, 656
RsaI	GTAC	6 cut(s)	480, 600, 620, 693, 753, 764
RsaNI	GTAC	6 cut(s)	479, 599, 619, 692, 752, 763
RseI	CAYNNNNRTG	2 cut(s)	101, 275
SaqAI	TTAA	1 cut(s)	1032
SatI	GCNGC	6 cut(s)	24, 144, 417, 508, 726, 1046
Sau3AI	GATC	4 cut(s)	644, 656, 804, 910
Sau96I	GGNCC	1 cut(s)	530
SchI	GAGTC	4 cut(s)	159, 161, 775, 866
SfaNI	GCATC	2 cut(s)	188, 916
SinI	GGWCC	1 cut(s)	530
SmiMI	CAYNNNNRTG	2 cut(s)	101, 275
SmlI	CTYRAG	2 cut(s)	172, 783
SmoI	CTYRAG	2 cut(s)	172, 783
Sse9I	AATT	3 cut(s)	482, 677, 845
SsiI	CCGC	5 cut(s)	138, 447, 569, 1025, 1045
SspMI	CTAG	6 cut(s)	420, 642, 660, 854, 971, 1090
StyI	CCWWGG	1 cut(s)	511
TaaI	ACNGT	4 cut(s)	13, 19, 48, 232
TaqI	TCGA	3 cut(s)	282, 913, 1073
TasI	AATT	3 cut(s)	482, 677, 845
TatI	WGTACW	4 cut(s)	478, 618, 691, 751
TauI	GCSGC	1 cut(s)	1048
Tru1I	TTAA	1 cut(s)	1032
Tru9I	TTAA	1 cut(s)	1032
TscAI	CASTG	2 cut(s)	411, 595
TseFI	GTSAC	1 cut(s)	13
TseI	GCWGC	5 cut(s)	23, 143, 416, 507, 725
Tsp45I	GTSAC	1 cut(s)	13
TspDTI	ATGAA	4 cut(s)	119, 245, 370, 897
TspGWI	ACGGA	1 cut(s)	689
TspRI	CASTG	2 cut(s)	411, 595
Van91I	CCANNNNNTGG	1 cut(s)	517
VpaK11BI	GGWCC	1 cut(s)	530
XapI	RAATTY	2 cut(s)	677, 845
XceI	RCATGY	1 cut(s)	280
XcmI	CCANNNNNNNNNTGG	1 cut(s)	819
XmnI	GAANNNNTTC	1 cut(s)	888
XspI	CTAG	6 cut(s)	420, 642, 660, 854, 971, 1090

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.