AT1G44180

Peptidase family M20/M25/M40

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0006711
Physical Location & Seq
Reverse (-)
16807828 .. 16810565
2738 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G44180.2

Sequence Viewer

Length: 1308 bp
ATGGCGGTGTCTCTTCTTCTCTGGACTCTCTTCCTCTTCTCATTTATCTTCTCCCTCCAATCACACAGCAAAGAAGAAGAAGAAGACACACCAATCACACGATTCCAGCAATACTTAAGATTCAACACCGCTCATCCAAACCCTAACTACACCGCACCAATCTCTTTTCTTCTTGATCAAGCAAGATCAATTGGTCTTACTTCTCGAACCATCGAGTTTGTTCCTGGTAAGCCAGTTCTTCTCCTCACATGGCTAGGCTCAAACCTTAACCTTTCTTCAATTCTCTTCAACTCTCATCTCGATTCTGTTCCCGCGGAATCCGAGAAGTGGATACATCCACCATTCTCAGCTCACCGAACCATCGATGGTCATATCTACGCTCGCGGCGCGCAAGATGACAAGTGTATAGGAGTTCAGTATCTAGAAGCAATCAGGAACTTGAAATCAAGAAGCTTCTCTCCTCTTCGTACGATCCATATCTCTTACGTACCTGAAGAAGAGATCGGTGGATTCGGTGGGATGATGAAATTCGCGGCGTCTTCGGAGTTTAAAGATTTGAATTTGGGATTTGTTATGGATGAAGGACAAGCGAGTCCCGGAGATGAGTTCAGGGTGTTTTACGCTGAACGAACTCCTTGGCATCTCGCTATCCGAGCTAATGGTATGCCAGGTCATGGTGCTAAGCTTTATGATAATTCAGCTATGGAGAATTTGATGAAGAGTGTTGAGTTGATTTCTAGGTTTAGAGAGTCACAGTTTGATTTTGTTAAAGCTGGTAAAGCTGCGAATTCGGAGGTTATCTCTGTGAATCCAGTTTATCTTAAAGCTGGAACTCCCTCCACTACTGGATTTGTGATGAACATGCAACCTTCAGAAGCAGAAGTTGGGTATGATCTAAGATTGCCTCCAATGGCAGATCCAGTTGCTTTGAAGAAAAGGATTGCTGAGGAATGGGCTCCTTCTATTAGGAACATGACCTACACGCTAAAACAACAAGGAAAGTTAACAGACCATTTAGGGCGACCTATAATGACTACAACAAATGATACCAATCCTTGGTGGTCTATCTTCAAGCAAGCTGTTGAAGCAACTGGAGGAAAACTCGCAAAGCCTGAAATATTGATTTCAACTACAGATTCACGCTTTATTCGCACTTTAGGAATACCAGTTCTAGGTTTCTCTCCAATGATCAATACACCTATTTTAGTGCATGACCATAACGAGTTCCTTAAAGATACCGTGTTCATGAAAGGAATAGGAGTATATGAATCAGTTATCAGCGCTCTAAGTTCCTTTGAGGCTGCTTGA

Protein Analysis

435

Amino Acids

48.61

Weight (kDa)

6.2

Isoelectric Point (pI)

43.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M20 PF01546 95 - 427 5.7e-30 Peptidase family M20/M25/M40
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 591
AccB7I CCANNNNNTGG 2 cut(s) 674, 1056
AccBSI CCGCTC 1 cut(s) 131
AccII CGCG 4 cut(s) 314, 384, 389, 533
AciI CCGC 7 cut(s) 5, 129, 153, 312, 314, 384, 533
AclWI GGATC 2 cut(s) 466, 911
AcsI RAATTY 4 cut(s) 527, 559, 709, 787
AcuI CTGAAG 2 cut(s) 513, 855
AcyI GRCGYC 1 cut(s) 536
AfaI GTAC 2 cut(s) 469, 489
AfeI AGCGCT 1 cut(s) 1282
AfiI CCNNNNNNNGG 4 cut(s) 327, 674, 1056, 1172
AflII CTTAAG 1 cut(s) 115
AgsI TTSAA 9 cut(s) 124, 279, 289, 442, 559, 931, 1072, 1085, 1128
AjnI CCWGG 2 cut(s) 223, 667
AjuI GAANNNNNNNTTGG 2 cut(s) 867, 899
AloI GAACNNNNNNTCC 2 cut(s) 1152, 1184
AluBI AGCT 9 cut(s) 350, 453, 656, 685, 701, 773, 782, 827, 1079
AluI AGCT 9 cut(s) 350, 453, 656, 685, 701, 773, 782, 827, 1079
Alw26I GTCTC 1 cut(s) 15
AlwI GGATC 2 cut(s) 466, 911
Aor51HI AGCGCT 1 cut(s) 1282
ApeKI GCWGC 2 cut(s) 782, 1301
ApoI RAATTY 4 cut(s) 527, 559, 709, 787
AspLEI GCGC 3 cut(s) 389, 391, 1283
AsuC2I CCSGG 1 cut(s) 597
AsuHPI GGTGA 1 cut(s) 344
BanII GRGCYC 1 cut(s) 958
BbsI GAAGAC 2 cut(s) 90, 531
BbvCI CCTCAGC 1 cut(s) 945
BbvI GCAGC 2 cut(s) 769, 1288
BccI CCATC 3 cut(s) 218, 359, 368
BciT130I CCWGG 2 cut(s) 225, 669
BciVI GTATCC 1 cut(s) 324
BclI TGATCA 2 cut(s) 175, 1188
BcnI CCSGG 1 cut(s) 597
BcoDI GTCTC 1 cut(s) 15
BfaI CTAG 4 cut(s) 254, 422, 738, 1172
BfmI CTRYAG 1 cut(s) 1131
BfoI RGCGCY 1 cut(s) 1284
BfrI CTTAAG 1 cut(s) 115
BfuI GTATCC 1 cut(s) 324
BisI GCNGC 4 cut(s) 385, 534, 783, 1302
BlpI GCTNAGC 1 cut(s) 681
BlsI GCNGC 4 cut(s) 386, 535, 784, 1303
Bme1390I CCNGG 3 cut(s) 225, 597, 669
BmiI GGNNCC 1 cut(s) 957
BmrFI CCNGG 3 cut(s) 225, 597, 669
BmsI GCATC 1 cut(s) 649
BpiI GAAGAC 2 cut(s) 90, 531
BplI GAGNNNNNCTC 4 cut(s) 785, 817, 1086, 1118
BpmI CTGGAG 1 cut(s) 1113
Bpu10I CCTNAGC 1 cut(s) 945
Bpu1102I GCTNAGC 1 cut(s) 681
BpuMI CCSGG 1 cut(s) 597
Bsa29I ATCGAT 1 cut(s) 363
BsaAI YACGTR 1 cut(s) 487
BsaBI GATNNNNATC 2 cut(s) 476, 1050
BsaHI GRCGYC 1 cut(s) 536
BsaJI CCNNGG 3 cut(s) 312, 635, 1055
Bsc4I CCNNNNNNNGG 4 cut(s) 327, 674, 1056, 1172
Bse1I ACTGG 6 cut(s) 233, 812, 850, 920, 1096, 1166
Bse8I GATNNNNATC 2 cut(s) 476, 1050
BseBI CCWGG 2 cut(s) 225, 669
BseCI ATCGAT 1 cut(s) 363
BseDI CCNNGG 3 cut(s) 312, 635, 1055
BseGI GGATG 4 cut(s) 133, 334, 525, 583
BseJI GATNNNNATC 2 cut(s) 476, 1050
BseLI CCNNNNNNNGG 4 cut(s) 327, 674, 1056, 1172
BseMII CTCAG 2 cut(s) 360, 936
BseNI ACTGG 6 cut(s) 233, 812, 850, 920, 1096, 1166
BsePI GCGCGC 1 cut(s) 387
BseRI GAGGAG 2 cut(s) 233, 450
BseXI GCAGC 2 cut(s) 769, 1288
Bsh1236I CGCG 4 cut(s) 314, 384, 389, 533
BshVI ATCGAT 1 cut(s) 363
BsiSI CCGG 1 cut(s) 597
BsiWI CGTACG 1 cut(s) 467
BslFI GGGAC 1 cut(s) 579
BslI CCNNNNNNNGG 4 cut(s) 327, 674, 1056, 1172
BsmAI GTCTC 1 cut(s) 15
BsmFI GGGAC 1 cut(s) 579
Bsp1286I GDGCHC 1 cut(s) 958
Bsp143I GATC 7 cut(s) 175, 185, 471, 501, 892, 916, 1188
Bsp1720I GCTNAGC 1 cut(s) 681
BspACI CCGC 7 cut(s) 5, 129, 153, 312, 314, 384, 533
BspCNI CTCAG 2 cut(s) 359, 937
BspDI ATCGAT 1 cut(s) 363
BspFNI CGCG 4 cut(s) 314, 384, 389, 533
BspHI TCATGA 1 cut(s) 1245
BspLI GGNNCC 1 cut(s) 957
BspPI GGATC 2 cut(s) 466, 911
BspTI CTTAAG 1 cut(s) 115
BsrBI CCGCTC 1 cut(s) 131
BsrI ACTGG 6 cut(s) 233, 812, 850, 920, 1096, 1166
BssECI CCNNGG 3 cut(s) 312, 635, 1055
BssHII GCGCGC 1 cut(s) 387
BssMI GATC 7 cut(s) 175, 185, 471, 501, 892, 916, 1188
BssNI GRCGYC 1 cut(s) 536
BssT1I CCWWGG 2 cut(s) 635, 1055
Bst2UI CCWGG 2 cut(s) 225, 669
Bst4CI ACNGT 2 cut(s) 756, 1240
Bst6I CTCTTC 7 cut(s) 18, 35, 41, 290, 468, 492, 713
BstACI GRCGYC 1 cut(s) 536
BstAFI CTTAAG 1 cut(s) 115
BstBAI YACGTR 1 cut(s) 487
BstC8I GCNNGC 3 cut(s) 382, 389, 1077
BstDEI CTNAG 5 cut(s) 346, 681, 896, 945, 1286
BstDSI CCRYGG 1 cut(s) 312
BstF5I GGATG 4 cut(s) 133, 334, 525, 583
BstFNI CGCG 4 cut(s) 314, 384, 389, 533
BstH2I RGCGCY 1 cut(s) 1284
BstHHI GCGC 3 cut(s) 389, 391, 1283
BstKTI GATC 7 cut(s) 178, 188, 474, 504, 895, 919, 1191
BstMAI GTCTC 1 cut(s) 15
BstMBI GATC 7 cut(s) 175, 185, 471, 501, 892, 916, 1188
BstMWI GCNNNNNNNGC 5 cut(s) 386, 653, 779, 1085, 1149
BstNI CCWGG 2 cut(s) 225, 669
BstNSI RCATGY 1 cut(s) 865
BstSCI CCNGG 3 cut(s) 223, 595, 667
BstSFI CTRYAG 1 cut(s) 1131
BstSNI TACGTA 1 cut(s) 487
BstUI CGCG 4 cut(s) 314, 384, 389, 533
BstV1I GCAGC 2 cut(s) 769, 1288
BstV2I GAAGAC 2 cut(s) 90, 531
BstX2I RGATCY 1 cut(s) 916
BstYI RGATCY 1 cut(s) 916
Bsu15I ATCGAT 1 cut(s) 363
BsuI GTATCC 1 cut(s) 324
BsuTUI ATCGAT 1 cut(s) 363
BtgI CCRYGG 1 cut(s) 312
BtsCI GGATG 4 cut(s) 133, 334, 525, 583
Cac8I GCNNGC 3 cut(s) 382, 389, 1077
CciI TCATGA 1 cut(s) 1245
CfoI GCGC 3 cut(s) 389, 391, 1283
Cfr42I CCGCGG 1 cut(s) 315
ClaI ATCGAT 1 cut(s) 363
CseI GACGC 1 cut(s) 525
Csp6I GTAC 2 cut(s) 468, 488
CviAII CATG 6 cut(s) 249, 674, 862, 973, 1211, 1246
CviQI GTAC 2 cut(s) 468, 488
DdeI CTNAG 5 cut(s) 346, 681, 896, 945, 1286
DpnI GATC 7 cut(s) 177, 187, 473, 503, 894, 918, 1190
DpnII GATC 7 cut(s) 175, 185, 471, 501, 892, 916, 1188
DraI TTTAAA 1 cut(s) 550
DrdI GACNNNNNNGTC 1 cut(s) 591
DseDI GACNNNNNNGTC 1 cut(s) 591
Eam1104I CTCTTC 7 cut(s) 18, 35, 41, 290, 468, 492, 713
EarI CTCTTC 7 cut(s) 18, 35, 41, 290, 468, 492, 713
Eco105I TACGTA 1 cut(s) 487
Eco130I CCWWGG 2 cut(s) 635, 1055
Eco24I GRGCYC 1 cut(s) 958
Eco47III AGCGCT 1 cut(s) 1282
Eco57I CTGAAG 2 cut(s) 513, 855
EcoRI GAATTC 1 cut(s) 787
EcoRII CCWGG 2 cut(s) 223, 667
EcoT14I CCWWGG 2 cut(s) 635, 1055
EcoT38I GRGCYC 1 cut(s) 958
ErhI CCWWGG 2 cut(s) 635, 1055
FaeI CATG 6 cut(s) 252, 677, 865, 976, 1214, 1249
FaqI GGGAC 1 cut(s) 579
FatI CATG 6 cut(s) 248, 673, 861, 972, 1210, 1245
FauI CCCGC 1 cut(s) 319
FbaI TGATCA 2 cut(s) 175, 1188
Fnu4HI GCNGC 4 cut(s) 385, 534, 783, 1302
FokI GGATG 4 cut(s) 120, 321, 532, 590
FriOI GRGCYC 1 cut(s) 958
Fsp4HI GCNGC 4 cut(s) 385, 534, 783, 1302
FspBI CTAG 4 cut(s) 254, 422, 738, 1172
GlaI GCGC 3 cut(s) 388, 390, 1282
GluI GCNGC 4 cut(s) 385, 534, 783, 1302
GsuI CTGGAG 1 cut(s) 1113
HaeII RGCGCY 1 cut(s) 1284
HapII CCGG 1 cut(s) 597
HgaI GACGC 1 cut(s) 525
HhaI GCGC 3 cut(s) 389, 391, 1283
Hin1I GRCGYC 1 cut(s) 536
Hin1II CATG 6 cut(s) 252, 677, 865, 976, 1214, 1249
Hin6I GCGC 3 cut(s) 387, 389, 1281
HinP1I GCGC 3 cut(s) 387, 389, 1281
HincII GTYRAC 1 cut(s) 1005
HindII GTYRAC 1 cut(s) 1005
HindIII AAGCTT 2 cut(s) 451, 683
HpaI GTTAAC 1 cut(s) 1005
HpaII CCGG 1 cut(s) 597
HphI GGTGA 1 cut(s) 344
Hpy166II GTNNAC 1 cut(s) 1005
Hpy188I TCNGA 5 cut(s) 322, 544, 653, 793, 874
Hpy188III TCNNGA 8 cut(s) 22, 173, 204, 299, 422, 433, 447, 1246
Hpy8I GTNNAC 1 cut(s) 1005
HpyAV CCTTC 3 cut(s) 575, 879, 969
HpyCH4III ACNGT 2 cut(s) 756, 1240
HpyCH4IV ACGT 1 cut(s) 486
HpyCH4V TGCA 2 cut(s) 865, 1210
HpyF10VI GCNNNNNNNGC 5 cut(s) 386, 653, 779, 1085, 1149
HpyF3I CTNAG 5 cut(s) 346, 681, 896, 945, 1286
HpySE526I ACGT 1 cut(s) 486
Hsp92I GRCGYC 1 cut(s) 536
Hsp92II CATG 6 cut(s) 252, 677, 865, 976, 1214, 1249
HspAI GCGC 3 cut(s) 387, 389, 1281
Ksp22I TGATCA 2 cut(s) 175, 1188
KspAI GTTAAC 1 cut(s) 1005
KspI CCGCGG 1 cut(s) 315
Kzo9I GATC 7 cut(s) 175, 185, 471, 501, 892, 916, 1188
LmnI GCTCC 1 cut(s) 961
Lsp1109I GCAGC 2 cut(s) 769, 1288
LweI GCATC 1 cut(s) 649
MaeI CTAG 4 cut(s) 254, 422, 738, 1172
MaeII ACGT 1 cut(s) 486
MaeIII GTNAC 1 cut(s) 750
MalI GATC 7 cut(s) 177, 187, 473, 503, 894, 918, 1190
MbiI CCGCTC 1 cut(s) 131
MboI GATC 7 cut(s) 175, 185, 471, 501, 892, 916, 1188
MfeI CAATTG 1 cut(s) 189
MflI RGATCY 1 cut(s) 916
MhlI GDGCHC 1 cut(s) 958
MluCI AATT 7 cut(s) 189, 279, 527, 559, 694, 709, 787
MlyI GAGTC 3 cut(s) 19, 601, 758
MseI TTAA 7 cut(s) 116, 267, 549, 768, 822, 1004, 1230
MspA1I CMGCKG 1 cut(s) 314
MspCI CTTAAG 1 cut(s) 115
MspI CCGG 1 cut(s) 597
MspR9I CCNGG 3 cut(s) 225, 597, 669
MunI CAATTG 1 cut(s) 189
MvaI CCWGG 2 cut(s) 225, 669
MvnI CGCG 4 cut(s) 314, 384, 389, 533
MwoI GCNNNNNNNGC 5 cut(s) 386, 653, 779, 1085, 1149
NciI CCSGG 1 cut(s) 597
NdeII GATC 7 cut(s) 175, 185, 471, 501, 892, 916, 1188
NlaIII CATG 6 cut(s) 252, 677, 865, 976, 1214, 1249
NlaIV GGNNCC 1 cut(s) 957
NmuCI GTSAC 1 cut(s) 750
NspI RCATGY 1 cut(s) 865
PagI TCATGA 1 cut(s) 1245
PauI GCGCGC 1 cut(s) 387
PfeI GAWTC 8 cut(s) 102, 120, 302, 317, 510, 808, 1136, 1268
Pfl23II CGTACG 1 cut(s) 467
PflMI CCANNNNNTGG 2 cut(s) 674, 1056
PfoI TCCNGGA 1 cut(s) 595
PkrI GCNGC 4 cut(s) 386, 535, 784, 1303
PleI GAGTC 3 cut(s) 19, 600, 757
PpsI GAGTC 3 cut(s) 19, 600, 757
Ppu21I YACGTR 1 cut(s) 487
Psp6I CCWGG 2 cut(s) 223, 667
PspGI CCWGG 2 cut(s) 223, 667
PspLI CGTACG 1 cut(s) 467
PspN4I GGNNCC 1 cut(s) 957
PsrI GAACNNNNNNTAC 2 cut(s) 962, 994
PsuI RGATCY 1 cut(s) 916
PteI GCGCGC 1 cut(s) 387
RsaI GTAC 2 cut(s) 469, 489
RsaNI GTAC 2 cut(s) 468, 488
SacII CCGCGG 1 cut(s) 315
SaqAI TTAA 7 cut(s) 116, 267, 549, 768, 822, 1004, 1230
SatI GCNGC 4 cut(s) 385, 534, 783, 1302
Sau3AI GATC 7 cut(s) 175, 185, 471, 501, 892, 916, 1188
SchI GAGTC 3 cut(s) 19, 601, 758
ScrFI CCNGG 3 cut(s) 225, 597, 669
SduI GDGCHC 1 cut(s) 958
SfaNI GCATC 1 cut(s) 649
SfcI CTRYAG 1 cut(s) 1131
Sfr303I CCGCGG 1 cut(s) 315
SgrBI CCGCGG 1 cut(s) 315
SmlI CTYRAG 1 cut(s) 115
SmoI CTYRAG 1 cut(s) 115
SnaBI TACGTA 1 cut(s) 487
Sse9I AATT 7 cut(s) 189, 279, 527, 559, 694, 709, 787
SsiI CCGC 7 cut(s) 5, 129, 153, 312, 314, 384, 533
SspI AATATT 1 cut(s) 1119
SspMI CTAG 4 cut(s) 254, 422, 738, 1172
StyD4I CCNGG 3 cut(s) 223, 595, 667
StyI CCWWGG 2 cut(s) 635, 1055
TaaI ACNGT 2 cut(s) 756, 1240
TaiI ACGT 1 cut(s) 489
TaqI TCGA 4 cut(s) 205, 213, 300, 363
TasI AATT 7 cut(s) 189, 279, 527, 559, 694, 709, 787
TauI GCSGC 2 cut(s) 387, 536
TfiI GAWTC 8 cut(s) 102, 120, 302, 317, 510, 808, 1136, 1268
Tru1I TTAA 7 cut(s) 116, 267, 549, 768, 822, 1004, 1230
Tru9I TTAA 7 cut(s) 116, 267, 549, 768, 822, 1004, 1230
TseFI GTSAC 1 cut(s) 750
TseI GCWGC 2 cut(s) 782, 1301
Tsp45I GTSAC 1 cut(s) 750
TspDTI ATGAA 7 cut(s) 539, 594, 731, 872, 1234, 1262, 1281
Van91I CCANNNNNTGG 2 cut(s) 674, 1056
Vha464I CTTAAG 1 cut(s) 115
XapI RAATTY 4 cut(s) 527, 559, 709, 787
XbaI TCTAGA 1 cut(s) 421
XceI RCATGY 1 cut(s) 865
XspI CTAG 4 cut(s) 254, 422, 738, 1172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.