AT1G44820

Peptidase family M20/M25/M40

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0006711
Physical Location & Seq
Forward (+)
16926297 .. 16929196
2900 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G44820.1

Sequence Viewer

Length: 1317 bp
ATGGCGATATCTCCTCTTCTCTGGACTCTTATCATCTTCTCCATAATCTTCTCACTCCAATCCTCCTCCTCCGAAGAAGACACACCAATCACACGATTCCAACAATACTTAAGATTCAACACAGCTCATCCAAACCCTAACTACACCGCACCAATCTCCTTCCTCATTAACCAAGCTCAATCAATCGGTCTCACTACCAAAACAATCGAATTCATCTCTGGCAAACCAATTCTTCTCATCACATGGCTAGGCTCAAACCCTAACCTCCCATCAATTCTCTTCAACTCTCATCTCGATTCAGTCCCCGCCGAATCAGAGAAATGGACATATCCACCGTTCTCAGCTCACAAAACCATCGACGGCCACATCTACGCTCGAGGAGCGCAAGACGACAAGTGTATCGGAGTTCAATATCTAGAATCAATCAGAAACTTGAAATCGAGAGGCTTCTCACCTCTCCGTACGATTCATATCTCTTACGTTCCTGAAGAAGAGATCGGTGGATTCGACGGGATGATGAAATTCGCGGCGTCATCGGAGTTTAAGGATTTGAATTTGGGATTTGCTATGGATGAAGGACAAGCTAATCCTGGCGATGAATTTAGGGTTTTTTACGCTGATCGAGTTCCATGGCATTTTGTTATTAAAGCTGAGGGGATTCCAGGTCATGGCGCTAAGCTTTATGATAATTCAGCTATGGAGAATTTGATGAAGAGTGTTGAGTTGATTTCTAGGTTTAGAGAGTCACAGTTTGATTTTGTTAAAGCTGGTAAAGCTGCGTATTCGGAGGTTATCTCTGTGAATCCAGTTTATCTTAAAGCTGGAACTCCTACTACTACTGGGTTTGTGATGAATATGCAGCCTTCAGAAGCAGAAGCTGGGTATGATTTAAGGTTGCCTCCAATGGCAGATCCTGATGTTATGAAGAAAAGAATTGCTGAAGAATGGGCTCCTTCTATTAGGAACATGACCTACTCGATACAAGAGAAAGGAAAGTTAAGAGATCATTTAGGGCGACCTATAATGACTCCAGTAAATGATTCTAATCCTTGGTGGTCTATCTTCAAGCAAGCTGTTGAAGCAATGGGAGGAAAACTCGCAAAGCCTGAAATTTTGGCTTCAACTACAGATTCACGCTTTATTCGCACTTTGGGAATTCCAACTTTTGGGTTCTCTCCAATGACCAATACGCCTATTTTATTGCATGACCATAACGAGTTCCTAAAGGATACTGTATTCATGAAAGGAATAGAAGTATATGAATCGGTTATCAGCGCTCTAAGTTCCTTTGAGGGAGAATCTGCTCAAGTGATTTGA

Protein Analysis

438

Amino Acids

48.96

Weight (kDa)

5.65

Isoelectric Point (pI)

43.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M20 PF01546 93 - 425 4.9e-31 Peptidase family M20/M25/M40
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 668, 1166
AccII CGCG 1 cut(s) 527
AciI CCGC 3 cut(s) 147, 306, 527
AclWI GGATC 1 cut(s) 905
AcoI YGGCCR 1 cut(s) 361
AcsI RAATTY 7 cut(s) 209, 521, 553, 599, 703, 1110, 1155
AcuI CTGAAG 3 cut(s) 507, 849, 960
AcyI GRCGYC 1 cut(s) 530
AfaI GTAC 1 cut(s) 463
AfeI AGCGCT 1 cut(s) 1276
AfiI CCNNNNNNNGG 2 cut(s) 668, 1166
AflII CTTAAG 1 cut(s) 109
AgsI TTSAA 8 cut(s) 118, 283, 410, 436, 553, 1066, 1079, 1122
AjnI CCWGG 2 cut(s) 589, 661
Alw26I GTCTC 1 cut(s) 194
AlwI GGATC 1 cut(s) 905
AlwNI CAGNNNCTG 2 cut(s) 878, 914
Ama87I CYCGRG 1 cut(s) 375
Aor51HI AGCGCT 1 cut(s) 1276
AoxI GGCC 1 cut(s) 361
ApeKI GCWGC 2 cut(s) 776, 859
ApoI RAATTY 7 cut(s) 209, 521, 553, 599, 703, 1110, 1155
AspLEI GCGC 3 cut(s) 385, 674, 1277
AsuHPI GGTGA 1 cut(s) 444
AvaI CYCGRG 1 cut(s) 375
BaeI ACNNNNGTAYC 2 cut(s) 382, 415
BanII GRGCYC 1 cut(s) 952
BbsI GAAGAC 1 cut(s) 84
BbvCI CCTCAGC 1 cut(s) 651
BbvI GCAGC 2 cut(s) 763, 871
BccI CCATC 2 cut(s) 277, 362
BceAI ACGGC 1 cut(s) 376
BciT130I CCWGG 2 cut(s) 591, 663
BciVI GTATCC 1 cut(s) 1222
BcoDI GTCTC 1 cut(s) 194
BfaI CTAG 3 cut(s) 248, 416, 732
BfmI CTRYAG 1 cut(s) 1125
BfoI RGCGCY 2 cut(s) 675, 1278
BfrI CTTAAG 1 cut(s) 109
BfuI GTATCC 1 cut(s) 1222
BisI GCNGC 3 cut(s) 528, 777, 860
BlpI GCTNAGC 1 cut(s) 675
BlsI GCNGC 3 cut(s) 529, 778, 861
Bme1390I CCNGG 2 cut(s) 591, 663
BmeT110I CYCGRG 1 cut(s) 375
BmiI GGNNCC 1 cut(s) 951
BmrFI CCNGG 2 cut(s) 591, 663
BmrI ACTGGG 1 cut(s) 849
BmuI ACTGGG 1 cut(s) 849
BpiI GAAGAC 1 cut(s) 84
BplI GAGNNNNNCTC 4 cut(s) 779, 811, 1080, 1112
BpmI CTGGAG 1 cut(s) 1014
Bpu10I CCTNAGC 1 cut(s) 651
Bpu1102I GCTNAGC 1 cut(s) 675
BpuEI CTTGAG 1 cut(s) 1290
BsaBI GATNNNNATC 2 cut(s) 470, 1044
BsaHI GRCGYC 1 cut(s) 530
BsaI GGTCTC 1 cut(s) 194
BsaJI CCNNGG 2 cut(s) 629, 1049
Bsc4I CCNNNNNNNGG 2 cut(s) 668, 1166
Bse1I ACTGG 3 cut(s) 806, 844, 1031
Bse3DI GCAATG 1 cut(s) 1089
Bse8I GATNNNNATC 2 cut(s) 470, 1044
BseBI CCWGG 2 cut(s) 591, 663
BseDI CCNNGG 2 cut(s) 629, 1049
BseGI GGATG 3 cut(s) 127, 519, 577
BseJI GATNNNNATC 2 cut(s) 470, 1044
BseLI CCNNNNNNNGG 2 cut(s) 668, 1166
BseMI GCAATG 1 cut(s) 1089
BseMII CTCAG 2 cut(s) 354, 642
BseNI ACTGG 3 cut(s) 806, 844, 1031
BseRI GAGGAG 3 cut(s) 55, 58, 393
BseXI GCAGC 2 cut(s) 763, 871
BseYI CCCAGC 1 cut(s) 878
Bsh1236I CGCG 1 cut(s) 527
BshFI GGCC 1 cut(s) 363
BsiHKCI CYCGRG 1 cut(s) 375
BsiWI CGTACG 1 cut(s) 461
BslFI GGGAC 1 cut(s) 287
BslI CCNNNNNNNGG 2 cut(s) 668, 1166
BsmAI GTCTC 1 cut(s) 194
BsmFI GGGAC 1 cut(s) 287
BsnI GGCC 1 cut(s) 363
Bso31I GGTCTC 1 cut(s) 194
BsoBI CYCGRG 1 cut(s) 375
Bsp1286I GDGCHC 1 cut(s) 952
Bsp143I GATC 4 cut(s) 495, 619, 910, 1003
Bsp1720I GCTNAGC 1 cut(s) 675
Bsp19I CCATGG 1 cut(s) 629
BspACI CCGC 3 cut(s) 147, 306, 527
BspANI GGCC 1 cut(s) 363
BspCNI CTCAG 2 cut(s) 353, 643
BspFNI CGCG 1 cut(s) 527
BspHI TCATGA 1 cut(s) 1239
BspLI GGNNCC 1 cut(s) 951
BspPI GGATC 1 cut(s) 905
BspTI CTTAAG 1 cut(s) 109
BspTNI GGTCTC 1 cut(s) 194
BsrDI GCAATG 1 cut(s) 1089
BsrI ACTGG 3 cut(s) 806, 844, 1031
BssECI CCNNGG 2 cut(s) 629, 1049
BssMI GATC 4 cut(s) 495, 619, 910, 1003
BssNI GRCGYC 1 cut(s) 530
BssT1I CCWWGG 2 cut(s) 629, 1049
Bst2UI CCWGG 2 cut(s) 591, 663
Bst4CI ACNGT 3 cut(s) 336, 750, 1234
Bst6I CTCTTC 4 cut(s) 21, 284, 486, 707
BstACI GRCGYC 1 cut(s) 530
BstAFI CTTAAG 1 cut(s) 109
BstC8I GCNNGC 1 cut(s) 1071
BstDEI CTNAG 4 cut(s) 340, 651, 675, 1280
BstDSI CCRYGG 1 cut(s) 629
BstF5I GGATG 3 cut(s) 127, 519, 577
BstFNI CGCG 1 cut(s) 527
BstH2I RGCGCY 2 cut(s) 675, 1278
BstHHI GCGC 3 cut(s) 385, 674, 1277
BstKTI GATC 4 cut(s) 498, 622, 913, 1006
BstMAI GTCTC 1 cut(s) 194
BstMBI GATC 4 cut(s) 495, 619, 910, 1003
BstMWI GCNNNNNNNGC 4 cut(s) 380, 773, 1079, 1143
BstNI CCWGG 2 cut(s) 591, 663
BstSCI CCNGG 2 cut(s) 589, 661
BstSFI CTRYAG 1 cut(s) 1125
BstUI CGCG 1 cut(s) 527
BstV1I GCAGC 2 cut(s) 763, 871
BstV2I GAAGAC 1 cut(s) 84
BstX2I RGATCY 1 cut(s) 910
BstYI RGATCY 1 cut(s) 910
BsuI GTATCC 1 cut(s) 1222
BsuRI GGCC 1 cut(s) 363
BtgI CCRYGG 1 cut(s) 629
BtgZI GCGATG 1 cut(s) 609
BtsCI GGATG 3 cut(s) 127, 519, 577
Cac8I GCNNGC 1 cut(s) 1071
CaiI CAGNNNCTG 2 cut(s) 878, 914
CciI TCATGA 1 cut(s) 1239
CfoI GCGC 3 cut(s) 385, 674, 1277
CseI GACGC 1 cut(s) 519
Csp6I GTAC 1 cut(s) 462
CviAII CATG 6 cut(s) 243, 630, 668, 967, 1205, 1240
CviQI GTAC 1 cut(s) 462
DdeI CTNAG 4 cut(s) 340, 651, 675, 1280
DpnI GATC 4 cut(s) 497, 621, 912, 1005
DpnII GATC 4 cut(s) 495, 619, 910, 1003
EaeI YGGCCR 1 cut(s) 361
Eam1104I CTCTTC 4 cut(s) 21, 284, 486, 707
EarI CTCTTC 4 cut(s) 21, 284, 486, 707
Eco130I CCWWGG 2 cut(s) 629, 1049
Eco24I GRGCYC 1 cut(s) 952
Eco31I GGTCTC 1 cut(s) 194
Eco32I GATATC 1 cut(s) 9
Eco47III AGCGCT 1 cut(s) 1276
Eco57I CTGAAG 3 cut(s) 507, 849, 960
Eco88I CYCGRG 1 cut(s) 375
EcoRI GAATTC 2 cut(s) 209, 1155
EcoRII CCWGG 2 cut(s) 589, 661
EcoRV GATATC 1 cut(s) 9
EcoT14I CCWWGG 2 cut(s) 629, 1049
EcoT38I GRGCYC 1 cut(s) 952
ErhI CCWWGG 2 cut(s) 629, 1049
FaeI CATG 6 cut(s) 246, 633, 671, 970, 1208, 1243
FaqI GGGAC 1 cut(s) 287
FatI CATG 6 cut(s) 242, 629, 667, 966, 1204, 1239
FauI CCCGC 1 cut(s) 313
Fnu4HI GCNGC 3 cut(s) 528, 777, 860
FokI GGATG 3 cut(s) 114, 526, 584
FriOI GRGCYC 1 cut(s) 952
Fsp4HI GCNGC 3 cut(s) 528, 777, 860
FspBI CTAG 3 cut(s) 248, 416, 732
GlaI GCGC 3 cut(s) 384, 673, 1276
GluI GCNGC 3 cut(s) 528, 777, 860
GsaI CCCAGC 1 cut(s) 882
GsuI CTGGAG 1 cut(s) 1014
HaeII RGCGCY 2 cut(s) 675, 1278
HaeIII GGCC 1 cut(s) 363
HgaI GACGC 1 cut(s) 519
HhaI GCGC 3 cut(s) 385, 674, 1277
Hin1I GRCGYC 1 cut(s) 530
Hin1II CATG 6 cut(s) 246, 633, 671, 970, 1208, 1243
Hin6I GCGC 3 cut(s) 383, 672, 1275
HinP1I GCGC 3 cut(s) 383, 672, 1275
HindIII AAGCTT 1 cut(s) 677
HphI GGTGA 1 cut(s) 444
Hpy188I TCNGA 7 cut(s) 73, 316, 404, 428, 538, 787, 868
Hpy188III TCNNGA 7 cut(s) 22, 293, 416, 441, 485, 914, 1240
Hpy99I CGWCG 2 cut(s) 362, 512
HpyAV CCTTC 4 cut(s) 169, 569, 873, 963
HpyCH4III ACNGT 3 cut(s) 336, 750, 1234
HpyCH4IV ACGT 1 cut(s) 480
HpyCH4V TGCA 2 cut(s) 859, 1204
HpyF10VI GCNNNNNNNGC 4 cut(s) 380, 773, 1079, 1143
HpyF3I CTNAG 4 cut(s) 340, 651, 675, 1280
HpySE526I ACGT 1 cut(s) 480
Hsp92I GRCGYC 1 cut(s) 530
Hsp92II CATG 6 cut(s) 246, 633, 671, 970, 1208, 1243
HspAI GCGC 3 cut(s) 383, 672, 1275
Kzo9I GATC 4 cut(s) 495, 619, 910, 1003
LmnI GCTCC 2 cut(s) 380, 955
Lsp1109I GCAGC 2 cut(s) 763, 871
MaeI CTAG 3 cut(s) 248, 416, 732
MaeII ACGT 1 cut(s) 480
MaeIII GTNAC 1 cut(s) 744
MalI GATC 4 cut(s) 497, 621, 912, 1005
MboI GATC 4 cut(s) 495, 619, 910, 1003
MflI RGATCY 1 cut(s) 910
MhlI GDGCHC 1 cut(s) 952
MlyI GAGTC 3 cut(s) 19, 752, 1021
MmeI TCCRAC 2 cut(s) 124, 1184
MseI TTAA 8 cut(s) 110, 168, 543, 645, 762, 816, 890, 998
MspCI CTTAAG 1 cut(s) 109
MspR9I CCNGG 2 cut(s) 591, 663
MvaI CCWGG 2 cut(s) 591, 663
MvnI CGCG 1 cut(s) 527
MwoI GCNNNNNNNGC 4 cut(s) 380, 773, 1079, 1143
NcoI CCATGG 1 cut(s) 629
NdeII GATC 4 cut(s) 495, 619, 910, 1003
NlaIII CATG 6 cut(s) 246, 633, 671, 970, 1208, 1243
NlaIV GGNNCC 1 cut(s) 951
NmuCI GTSAC 1 cut(s) 744
PaeR7I CTCGAG 1 cut(s) 375
PagI TCATGA 1 cut(s) 1239
PcsI WCGNNNNNNNCGW 1 cut(s) 504
Pfl23II CGTACG 1 cut(s) 461
PflMI CCANNNNNTGG 2 cut(s) 668, 1166
PkrI GCNGC 3 cut(s) 529, 778, 861
PleI GAGTC 3 cut(s) 19, 751, 1021
PpsI GAGTC 3 cut(s) 19, 751, 1021
Psp6I CCWGG 2 cut(s) 589, 661
PspFI CCCAGC 1 cut(s) 878
PspGI CCWGG 2 cut(s) 589, 661
PspLI CGTACG 1 cut(s) 461
PspN4I GGNNCC 1 cut(s) 951
PspXI VCTCGAGB 1 cut(s) 375
PsrI GAACNNNNNNTAC 4 cut(s) 817, 849, 956, 988
PstNI CAGNNNCTG 2 cut(s) 878, 914
PsuI RGATCY 1 cut(s) 910
RsaI GTAC 1 cut(s) 463
RsaNI GTAC 1 cut(s) 462
SaqAI TTAA 8 cut(s) 110, 168, 543, 645, 762, 816, 890, 998
SatI GCNGC 3 cut(s) 528, 777, 860
Sau3AI GATC 4 cut(s) 495, 619, 910, 1003
SchI GAGTC 3 cut(s) 19, 752, 1021
ScrFI CCNGG 2 cut(s) 591, 663
SduI GDGCHC 1 cut(s) 952
SfcI CTRYAG 1 cut(s) 1125
Sfr274I CTCGAG 1 cut(s) 375
SlaI CTCGAG 1 cut(s) 375
SmlI CTYRAG 3 cut(s) 109, 375, 1305
SmoI CTYRAG 3 cut(s) 109, 375, 1305
SsiI CCGC 3 cut(s) 147, 306, 527
SspMI CTAG 3 cut(s) 248, 416, 732
StyD4I CCNGG 2 cut(s) 589, 661
StyI CCWWGG 2 cut(s) 629, 1049
TaaI ACNGT 3 cut(s) 336, 750, 1234
TaiI ACGT 1 cut(s) 483
TaqI TCGA 8 cut(s) 207, 294, 357, 376, 440, 507, 622, 977
TaqII GACCGA 1 cut(s) 176
TauI GCSGC 1 cut(s) 530
Tru1I TTAA 8 cut(s) 110, 168, 543, 645, 762, 816, 890, 998
Tru9I TTAA 8 cut(s) 110, 168, 543, 645, 762, 816, 890, 998
TseFI GTSAC 1 cut(s) 744
TseI GCWGC 2 cut(s) 776, 859
Tsp45I GTSAC 1 cut(s) 744
TspGWI ACGGA 1 cut(s) 449
Van91I CCANNNNNTGG 2 cut(s) 668, 1166
Vha464I CTTAAG 1 cut(s) 109
XapI RAATTY 7 cut(s) 209, 521, 553, 599, 703, 1110, 1155
XbaI TCTAGA 1 cut(s) 415
XhoI CTCGAG 1 cut(s) 375
XspI CTAG 3 cut(s) 248, 416, 732
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.