RLG00000028911

Peptidase family M20/M25/M40

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0006711
Physical Location & Seq
Reverse (-)
30150410 .. 30151902
1493 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028911

Sequence Viewer

Length: 600 bp
ATGAAACCCTGGCAATTGCAAAATGAAACCCTAGCAAACCCTAAAGAGAAACTAAAGAATGTAGATGGGCAAGCCGAGCCCTCCAAGTGGGCCCACTCACCCTTCTCGGCCCACCGCACCCCCGAAGGCCACATCTTCACTTGCGGCGCCCAGGACGACAAGTGCATCGCCATCCAGTACCTCGAGGCCTTCCGCAATCTCAAAGCCGCAGGCTTCAACCCAATCCGCACCTTCCACGTGTCCTACGTCCCCGACGAGGAGATCGGTGGCCTCGACGGCGCCGCCAAGTTAGCAGTCTCTGACGAGTTCAAGGAACTGAAGGTCGGGTTTGTTTTGGACGAGGGTCAGGCCAGCCCCGACAATGAGATTCGGGTCTTTTACACTGATCGGACGCTGTGGAATTTGATCGTCAAGGCGAAGGGGACACCAAAATCCAGGTGGGTCTTGTCGGAAATTCAGTCCGGGAATAACAGTATCATCAGGCTCTCTGGTTATTATTACTTGAGGAGGAGGAGGTTCAGGGTCTGCTGCAAAGCTCAAGAATCAGATAACAAAAGCAATGGCAAGCTCTTCAATTTTGCTATTTGGTTTCGTTTCTAG

Protein Analysis

200

Amino Acids

22.76

Weight (kDa)

9.14

Isoelectric Point (pI)

39.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M20 PF01546 25 - 112 4e-14 Peptidase family M20/M25/M40
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 182
AccB1I GGYRCC 2 cut(s) 146, 278
AciI CCGC 6 cut(s) 115, 144, 193, 207, 226, 282
AcsI RAATTY 2 cut(s) 400, 453
AcuI CTGAAG 1 cut(s) 338
AcvI CACGTG 1 cut(s) 238
AcyI GRCGYC 2 cut(s) 147, 279
AfaI GTAC 1 cut(s) 179
AfiI CCNNNNNNNGG 2 cut(s) 87, 256
AflIII ACRYGT 1 cut(s) 237
AgsI TTSAA 3 cut(s) 217, 310, 574
AjnI CCWGG 3 cut(s) 8, 150, 434
AluBI AGCT 2 cut(s) 536, 568
AluI AGCT 2 cut(s) 536, 568
Alw26I GTCTC 1 cut(s) 301
AlwNI CAGNNNCTG 2 cut(s) 299, 525
Ama87I CYCGRG 1 cut(s) 182
AoxI GGCC 6 cut(s) 90, 108, 127, 186, 268, 348
ApaI GGGCCC 1 cut(s) 94
ApeKI GCWGC 1 cut(s) 528
ApoI RAATTY 2 cut(s) 400, 453
AspLEI GCGC 2 cut(s) 149, 281
AspS9I GGNCC 3 cut(s) 90, 91, 109
AsuC2I CCSGG 1 cut(s) 463
AsuHPI GGTGA 1 cut(s) 90
AvaI CYCGRG 1 cut(s) 182
BaeGI GKGCMC 1 cut(s) 94
BanI GGYRCC 2 cut(s) 146, 278
BanII GRGCYC 2 cut(s) 81, 94
BbrPI CACGTG 1 cut(s) 238
BbvI GCAGC 1 cut(s) 515
BccI CCATC 2 cut(s) 59, 179
BceAI ACGGC 1 cut(s) 292
BciT130I CCWGG 3 cut(s) 10, 152, 436
BcnI CCSGG 1 cut(s) 463
BcoDI GTCTC 1 cut(s) 301
BfaI CTAG 2 cut(s) 32, 598
BfoI RGCGCY 2 cut(s) 150, 282
BglI GCCNNNNNGGC 1 cut(s) 276
BisI GCNGC 4 cut(s) 145, 207, 282, 529
BlsI GCNGC 4 cut(s) 146, 208, 283, 530
Bme1390I CCNGG 4 cut(s) 10, 152, 436, 463
BmeT110I CYCGRG 1 cut(s) 182
BmgT120I GGNCC 3 cut(s) 90, 91, 109
BmiI GGNNCC 3 cut(s) 92, 148, 280
BmrFI CCNGG 4 cut(s) 10, 152, 436, 463
BmsI GCATC 1 cut(s) 174
BoxI GACNNNNGTC 1 cut(s) 342
BpuEI CTTGAG 2 cut(s) 522, 523
BpuMI CCSGG 1 cut(s) 463
BsaAI YACGTR 1 cut(s) 238
BsaHI GRCGYC 2 cut(s) 147, 279
BsaJI CCNNGG 2 cut(s) 8, 150
Bsc4I CCNNNNNNNGG 2 cut(s) 87, 256
Bse1I ACTGG 1 cut(s) 175
Bse3DI GCAATG 1 cut(s) 565
BseBI CCWGG 3 cut(s) 10, 152, 436
BseDI CCNNGG 2 cut(s) 8, 150
BseGI GGATG 1 cut(s) 171
BseLI CCNNNNNNNGG 2 cut(s) 87, 256
BseMI GCAATG 1 cut(s) 565
BseNI ACTGG 1 cut(s) 175
BseRI GAGGAG 4 cut(s) 272, 520, 523, 526
BseSI GKGCMC 1 cut(s) 94
BseXI GCAGC 1 cut(s) 515
BshFI GGCC 6 cut(s) 92, 110, 129, 188, 270, 350
BshNI GGYRCC 2 cut(s) 146, 278
BsiHKCI CYCGRG 1 cut(s) 182
BsiSI CCGG 1 cut(s) 462
BslFI GGGAC 2 cut(s) 233, 436
BslI CCNNNNNNNGG 2 cut(s) 87, 256
BsmAI GTCTC 1 cut(s) 301
BsmFI GGGAC 2 cut(s) 233, 436
BsnI GGCC 6 cut(s) 92, 110, 129, 188, 270, 350
BsoBI CYCGRG 1 cut(s) 182
Bsp120I GGGCCC 1 cut(s) 90
Bsp1286I GDGCHC 2 cut(s) 81, 94
Bsp143I GATC 3 cut(s) 261, 385, 405
BspACI CCGC 6 cut(s) 115, 144, 193, 207, 226, 282
BspANI GGCC 6 cut(s) 92, 110, 129, 188, 270, 350
BspLI GGNNCC 3 cut(s) 92, 148, 280
BspQI GCTCTTC 1 cut(s) 575
BspT107I GGYRCC 2 cut(s) 146, 278
BsrDI GCAATG 1 cut(s) 565
BsrI ACTGG 1 cut(s) 175
BssECI CCNNGG 2 cut(s) 8, 150
BssMI GATC 3 cut(s) 261, 385, 405
BssNI GRCGYC 2 cut(s) 147, 279
Bst2UI CCWGG 3 cut(s) 10, 152, 436
Bst4CI ACNGT 1 cut(s) 473
Bst6I CTCTTC 1 cut(s) 575
BstACI GRCGYC 2 cut(s) 147, 279
BstBAI YACGTR 1 cut(s) 238
BstC8I GCNNGC 4 cut(s) 72, 211, 352, 566
BstF5I GGATG 1 cut(s) 171
BstH2I RGCGCY 2 cut(s) 150, 282
BstHHI GCGC 2 cut(s) 149, 281
BstKTI GATC 3 cut(s) 264, 388, 408
BstMAI GTCTC 1 cut(s) 301
BstMBI GATC 3 cut(s) 261, 385, 405
BstMWI GCNNNNNNNGC 3 cut(s) 76, 276, 290
BstNI CCWGG 3 cut(s) 10, 152, 436
BstPAI GACNNNNGTC 1 cut(s) 342
BstSCI CCNGG 4 cut(s) 8, 150, 434, 461
BstSLI GKGCMC 1 cut(s) 94
BstV1I GCAGC 1 cut(s) 515
BsuRI GGCC 6 cut(s) 92, 110, 129, 188, 270, 350
BtgZI GCGATG 1 cut(s) 151
BtsCI GGATG 1 cut(s) 171
BtsIMutI CAGTG 1 cut(s) 381
Cac8I GCNNGC 4 cut(s) 72, 211, 352, 566
CaiI CAGNNNCTG 2 cut(s) 299, 525
CfoI GCGC 2 cut(s) 149, 281
Cfr13I GGNCC 3 cut(s) 90, 91, 109
CseI GACGC 1 cut(s) 400
Csp6I GTAC 1 cut(s) 178
CviQI GTAC 1 cut(s) 178
DinI GGCGCC 2 cut(s) 148, 280
DpnI GATC 3 cut(s) 263, 387, 407
DpnII GATC 3 cut(s) 261, 385, 405
Eam1104I CTCTTC 1 cut(s) 575
EarI CTCTTC 1 cut(s) 575
Eco147I AGGCCT 1 cut(s) 188
Eco24I GRGCYC 2 cut(s) 81, 94
Eco57I CTGAAG 1 cut(s) 338
Eco72I CACGTG 1 cut(s) 238
Eco88I CYCGRG 1 cut(s) 182
EcoRII CCWGG 3 cut(s) 8, 150, 434
EcoT38I GRGCYC 2 cut(s) 81, 94
EgeI GGCGCC 2 cut(s) 148, 280
EheI GGCGCC 2 cut(s) 148, 280
FaqI GGGAC 2 cut(s) 233, 436
Fnu4HI GCNGC 4 cut(s) 145, 207, 282, 529
FokI GGATG 1 cut(s) 158
FriOI GRGCYC 2 cut(s) 81, 94
Fsp4HI GCNGC 4 cut(s) 145, 207, 282, 529
FspBI CTAG 2 cut(s) 32, 598
GlaI GCGC 2 cut(s) 148, 280
GluI GCNGC 4 cut(s) 145, 207, 282, 529
HaeII RGCGCY 2 cut(s) 150, 282
HaeIII GGCC 6 cut(s) 92, 110, 129, 188, 270, 350
HapII CCGG 1 cut(s) 462
HgaI GACGC 1 cut(s) 400
HhaI GCGC 2 cut(s) 149, 281
Hin1I GRCGYC 2 cut(s) 147, 279
Hin6I GCGC 2 cut(s) 147, 279
HinP1I GCGC 2 cut(s) 147, 279
HinfI GANTC 2 cut(s) 367, 542
HpaII CCGG 1 cut(s) 462
HphI GGTGA 1 cut(s) 90
Hpy188I TCNGA 4 cut(s) 301, 390, 451, 547
Hpy188III TCNNGA 1 cut(s) 539
Hpy99I CGWCG 2 cut(s) 257, 278
HpyAV CCTTC 6 cut(s) 112, 119, 199, 241, 313, 412
HpyCH4III ACNGT 1 cut(s) 473
HpyCH4IV ACGT 2 cut(s) 237, 246
HpyCH4V TGCA 3 cut(s) 19, 165, 531
HpyF10VI GCNNNNNNNGC 3 cut(s) 76, 276, 290
HpySE526I ACGT 2 cut(s) 237, 246
Hsp92I GRCGYC 2 cut(s) 147, 279
HspAI GCGC 2 cut(s) 147, 279
KasI GGCGCC 2 cut(s) 146, 278
Kzo9I GATC 3 cut(s) 261, 385, 405
LguI GCTCTTC 1 cut(s) 575
Lsp1109I GCAGC 1 cut(s) 515
LweI GCATC 1 cut(s) 174
MaeI CTAG 2 cut(s) 32, 598
MaeII ACGT 2 cut(s) 237, 246
MalI GATC 3 cut(s) 263, 387, 407
MboI GATC 3 cut(s) 261, 385, 405
MboII GAAGA 2 cut(s) 127, 562
MfeI CAATTG 1 cut(s) 14
MhlI GDGCHC 2 cut(s) 81, 94
MluCI AATT 4 cut(s) 14, 400, 453, 574
Mly113I GGCGCC 2 cut(s) 147, 279
MmeI TCCRAC 1 cut(s) 429
MspI CCGG 1 cut(s) 462
MspR9I CCNGG 4 cut(s) 10, 152, 436, 463
MunI CAATTG 1 cut(s) 14
MvaI CCWGG 3 cut(s) 10, 152, 436
MwoI GCNNNNNNNGC 3 cut(s) 76, 276, 290
NarI GGCGCC 2 cut(s) 147, 279
NciI CCSGG 1 cut(s) 463
NdeII GATC 3 cut(s) 261, 385, 405
NlaIV GGNNCC 3 cut(s) 92, 148, 280
NmeAIII GCCGAG 2 cut(s) 86, 100
PaeR7I CTCGAG 1 cut(s) 182
PceI AGGCCT 1 cut(s) 188
PciSI GCTCTTC 1 cut(s) 575
PcsI WCGNNNNNNNCGW 3 cut(s) 243, 252, 270
PfeI GAWTC 2 cut(s) 367, 542
PfoI TCCNGGA 1 cut(s) 461
PkrI GCNGC 4 cut(s) 146, 208, 283, 530
PluTI GGCGCC 2 cut(s) 150, 282
PmaCI CACGTG 1 cut(s) 238
PmlI CACGTG 1 cut(s) 238
Ppu21I YACGTR 1 cut(s) 238
PshAI GACNNNNGTC 1 cut(s) 342
Psp6I CCWGG 3 cut(s) 8, 150, 434
PspCI CACGTG 1 cut(s) 238
PspGI CCWGG 3 cut(s) 8, 150, 434
PspN4I GGNNCC 3 cut(s) 92, 148, 280
PspOMI GGGCCC 1 cut(s) 90
PspPI GGNCC 3 cut(s) 90, 91, 109
PspXI VCTCGAGB 1 cut(s) 182
PstNI CAGNNNCTG 2 cut(s) 299, 525
RsaI GTAC 1 cut(s) 179
RsaNI GTAC 1 cut(s) 178
SapI GCTCTTC 1 cut(s) 575
SatI GCNGC 4 cut(s) 145, 207, 282, 529
Sau3AI GATC 3 cut(s) 261, 385, 405
Sau96I GGNCC 3 cut(s) 90, 91, 109
ScrFI CCNGG 4 cut(s) 10, 152, 436, 463
SduI GDGCHC 2 cut(s) 81, 94
SetI ASST 9 cut(s) 183, 233, 240, 249, 324, 440, 518, 538, 570
SfaNI GCATC 1 cut(s) 174
SfoI GGCGCC 2 cut(s) 148, 280
Sfr274I CTCGAG 1 cut(s) 182
SlaI CTCGAG 1 cut(s) 182
SmlI CTYRAG 3 cut(s) 182, 502, 537
SmoI CTYRAG 3 cut(s) 182, 502, 537
Sse9I AATT 4 cut(s) 14, 400, 453, 574
SseBI AGGCCT 1 cut(s) 188
SsiI CCGC 6 cut(s) 115, 144, 193, 207, 226, 282
SspDI GGCGCC 2 cut(s) 146, 278
SspMI CTAG 2 cut(s) 32, 598
StuI AGGCCT 1 cut(s) 188
StyD4I CCNGG 4 cut(s) 8, 150, 434, 461
TaaI ACNGT 1 cut(s) 473
TaiI ACGT 2 cut(s) 240, 249
TaqI TCGA 2 cut(s) 183, 273
TasI AATT 4 cut(s) 14, 400, 453, 574
TauI GCSGC 3 cut(s) 147, 209, 284
TfiI GAWTC 2 cut(s) 367, 542
TscAI CASTG 1 cut(s) 388
TseI GCWGC 1 cut(s) 528
TspDTI ATGAA 2 cut(s) 17, 39
TspRI CASTG 1 cut(s) 388
XapI RAATTY 2 cut(s) 400, 453
XcmI CCANNNNNNNNNTGG 1 cut(s) 435
XhoI CTCGAG 1 cut(s) 182
XspI CTAG 2 cut(s) 32, 598
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.