AT1G48450

mRNA level of the MEB5.2 gene (At3g17800) remains unchanged after cutting the inflorescence stem

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0004076
Physical Location & Seq
Reverse (-)
17908503 .. 17910523
2021 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G48450.2

Sequence Viewer

Length: 1272 bp
ATGGACGCTGTGGCGGCGAATTTGTTTCAATCTCCTTCCTTACTGCGTCAAACTTCCGAAAGGTTGATATTTTCGACGCCCAACTGTCCTGGCTTCATGCGAATTAGCTCCGGACCTCAATTTCGTCTCAGACAAAACTCTTCTCTTAAATTCTCGAGACCATTTCAAAGCGGAACAACTTGTGTTAAATCTCGGAGAAGCTTTGTGGTTAAAGCTTCAGCTTCTGGTGATGCTTCAACAGAATCAATCGCGCCGCTTCAGTTGAAGTCTCCTGTGGGACAGTTTCTGTCACAGATATTAGTGAGCCATCCTCATCTTGTTCCAGCAGCTGTTGAACAGCAGCTTGAACAGCTCCAAATTGATCGAGACGCTGAAGAACAGAGTAAAGATGCATCCTCTGTCCTTGGAACAGACATTGTTCTCTACAGGAGAATTGCTGAAGTTAAGGAGAAAGAGAGAAGAAGGGCCTTGGAAGAGATTTTGTATGCGTTAGTGGTTCAGAAGTTCATGGATGCTAATGTTACACTTGTACCATCCATAACCTCTTCATCTGCGGATCCATCTGGTCGAGTTGATACTTGGCCAACTCTAGATGGGGAACTTGAGAGACTTCACTCGCCTGAAGTTTATGAGATGATTCAGAATCATCTTTCCATCATTCTTAAAAACCGCACTGATGATCTGACAGCAGTTGCGCAGATAAGCAAACTTGGAGTTGGACAGGTATATGCTGCTTCGGTGATGTATGGGTATTTCCTGAAGAGGATCGATCAAAGGTTTCAGCTTGAGAAGACGATGAGGATTCTCCCAGGTGGGTCAGATGAAGGTGAGACTAGCATTGAGCAAGCAGGCCGAGATGTAGAGAGAAACTTCTATGAAGAAGCAGAAGAGACTTATCAGGCTGTTTCCTCAAACCAAGACGTTGGTTCGTTTGTAGGAGGGATCAATGCCAGTGGCGGTTTTAGCAGTGATATGAAGCAATCCCGGTTAAAGACATACGTTATGTCCTTTGATGGAGAAACCCTTCAAAGGTACGCTACAATAAGATCTAGAGAATCGGTTGGGATAATTGAGAAGCATACAGAAGCATTGTTTGGGAGGCCAGAGATTGTGATAACGCCACAAGGCACTATTGATTCCTCGAAGGACGAGCATATCAAGATCAGCTTCAAGGGATTGAAGAGGCTTGTGTTGGAGGCTGTGACGTTTGGTTCTTTCCTCTGGGACGTGGAGAGCCATGTAGACTCAAGGTATCACTTCGTACTGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000302 GO:0001101 GO:0006950 GO:0006979 GO:0008150 GO:0009314 GO:0009411 GO:0009416 GO:0009611 GO:0009628 GO:0010035 GO:0010193 GO:0010224 GO:0042221 GO:0042493 GO:0046677 GO:0050896 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF760

Protein Analysis

423

Amino Acids

47.19

Weight (kDa)

5.86

Isoelectric Point (pI)

55.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF760 PF05542 141 - 266 8.1e-21 Protein of unknown function (DUF760)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 696
AccI GTMKAC 1 cut(s) 1242
AccII CGCG 1 cut(s) 251
AccIII TCCGGA 1 cut(s) 110
AciI CCGC 6 cut(s) 14, 171, 254, 554, 670, 957
AclWI GGATC 4 cut(s) 551, 564, 773, 950
AcoI YGGCCR 1 cut(s) 581
AcsI RAATTY 2 cut(s) 19, 149
AcuI CTGAAG 6 cut(s) 201, 242, 393, 459, 642, 779
AcyI GRCGYC 1 cut(s) 77
AfaI GTAC 3 cut(s) 531, 1034, 1263
AfiI CCNNNNNNNGG 1 cut(s) 1029
AgsI TTSAA 9 cut(s) 29, 167, 237, 265, 335, 347, 1028, 1171, 1180
AjiI CACGTC 1 cut(s) 1228
AjnI CCWGG 2 cut(s) 88, 808
AjuI GAANNNNNNNTTGG 2 cut(s) 1077, 1109
AluBI AGCT 9 cut(s) 108, 201, 215, 221, 329, 343, 352, 784, 1167
AluI AGCT 9 cut(s) 108, 201, 215, 221, 329, 343, 352, 784, 1167
Alw26I GTCTC 7 cut(s) 131, 151, 273, 360, 601, 824, 884
AlwI GGATC 4 cut(s) 551, 564, 773, 950
AlwNI CAGNNNCTG 3 cut(s) 224, 286, 329
Ama87I CYCGRG 1 cut(s) 154
Aor13HI TCCGGA 1 cut(s) 110
AoxI GGCC 4 cut(s) 465, 581, 850, 1100
ApeKI GCWGC 3 cut(s) 326, 340, 731
ApoI RAATTY 2 cut(s) 19, 149
ArsI GACNNNNNNTTYG 2 cut(s) 105, 137
Asp700I GAANNNNTTC 1 cut(s) 1023
AspLEI GCGC 2 cut(s) 253, 697
AspS9I GGNCC 2 cut(s) 113, 465
AsuC2I CCSGG 1 cut(s) 985
AsuHPI GGTGA 3 cut(s) 239, 751, 839
AvaI CYCGRG 1 cut(s) 154
AvaII GGWCC 1 cut(s) 113
BaeI ACNNNNGTAYC 2 cut(s) 513, 546
BalI TGGCCA 1 cut(s) 583
BamHI GGATCC 1 cut(s) 556
BbsI GAAGAC 1 cut(s) 797
BbvI GCAGC 3 cut(s) 338, 352, 718
BccI CCATC 6 cut(s) 315, 541, 568, 587, 662, 1007
BciT130I CCWGG 2 cut(s) 90, 810
BcnI CCSGG 1 cut(s) 985
BcoDI GTCTC 7 cut(s) 131, 151, 273, 360, 601, 824, 884
BfaI CTAG 3 cut(s) 590, 834, 1050
BfmI CTRYAG 1 cut(s) 424
BglII AGATCT 1 cut(s) 1046
BisI GCNGC 5 cut(s) 15, 254, 327, 341, 732
BlsI GCNGC 5 cut(s) 16, 255, 328, 342, 733
Bme1390I CCNGG 3 cut(s) 90, 810, 985
Bme18I GGWCC 1 cut(s) 113
BmeT110I CYCGRG 1 cut(s) 154
BmgBI CACGTC 1 cut(s) 1228
BmgT120I GGNCC 2 cut(s) 113, 465
BmiI GGNNCC 1 cut(s) 558
BmrFI CCNGG 3 cut(s) 90, 810, 985
BmsI GCATC 4 cut(s) 220, 379, 401, 502
BpiI GAAGAC 1 cut(s) 797
BplI GAGNNNNNCTC 2 cut(s) 295, 327
BpuEI CTTGAG 3 cut(s) 623, 806, 1231
BpuMI CCSGG 1 cut(s) 985
Bsa29I ATCGAT 1 cut(s) 768
BsaHI GRCGYC 1 cut(s) 77
BsaI GGTCTC 1 cut(s) 151
BsaJI CCNNGG 3 cut(s) 403, 468, 808
BsaWI WCCGGW 1 cut(s) 110
Bsc4I CCNNNNNNNGG 1 cut(s) 1029
Bse1I ACTGG 1 cut(s) 951
BseAI TCCGGA 1 cut(s) 110
BseBI CCWGG 2 cut(s) 90, 810
BseCI ATCGAT 1 cut(s) 768
BseDI CCNNGG 3 cut(s) 403, 468, 808
BseGI GGATG 4 cut(s) 307, 392, 517, 533
BseLI CCNNNNNNNGG 1 cut(s) 1029
BseMII CTCAG 1 cut(s) 142
BseNI ACTGG 1 cut(s) 951
BseXI GCAGC 3 cut(s) 338, 352, 718
Bsh1236I CGCG 1 cut(s) 251
BshFI GGCC 4 cut(s) 467, 583, 852, 1102
BshVI ATCGAT 1 cut(s) 768
BsiHKCI CYCGRG 1 cut(s) 154
BsiSI CCGG 2 cut(s) 111, 985
BslFI GGGAC 2 cut(s) 291, 1238
BslI CCNNNNNNNGG 1 cut(s) 1029
BsmAI GTCTC 7 cut(s) 131, 151, 273, 360, 601, 824, 884
BsmBI CGTCTC 2 cut(s) 131, 360
BsmFI GGGAC 2 cut(s) 291, 1238
BsnI GGCC 4 cut(s) 467, 583, 852, 1102
Bso31I GGTCTC 1 cut(s) 151
BsoBI CYCGRG 1 cut(s) 154
Bsp13I TCCGGA 1 cut(s) 110
Bsp143I GATC 8 cut(s) 361, 556, 679, 765, 769, 942, 1046, 1161
BspACI CCGC 6 cut(s) 14, 171, 254, 554, 670, 957
BspANI GGCC 4 cut(s) 467, 583, 852, 1102
BspCNI CTCAG 1 cut(s) 141
BspDI ATCGAT 1 cut(s) 768
BspEI TCCGGA 1 cut(s) 110
BspFNI CGCG 1 cut(s) 251
BspLI GGNNCC 1 cut(s) 558
BspPI GGATC 4 cut(s) 551, 564, 773, 950
BspTNI GGTCTC 1 cut(s) 151
BsrI ACTGG 1 cut(s) 951
BssECI CCNNGG 3 cut(s) 403, 468, 808
BssMI GATC 8 cut(s) 361, 556, 679, 765, 769, 942, 1046, 1161
BssNI GRCGYC 1 cut(s) 77
BssT1I CCWWGG 2 cut(s) 403, 468
Bst2UI CCWGG 2 cut(s) 90, 810
Bst4CI ACNGT 2 cut(s) 86, 282
Bst6I CTCTTC 6 cut(s) 145, 468, 550, 755, 882, 1175
BstACI GRCGYC 1 cut(s) 77
BstC8I GCNNGC 2 cut(s) 846, 850
BstDEI CTNAG 1 cut(s) 128
BstF5I GGATG 4 cut(s) 307, 392, 517, 533
BstFNI CGCG 1 cut(s) 251
BstHHI GCGC 2 cut(s) 253, 697
BstKTI GATC 8 cut(s) 364, 559, 682, 768, 772, 945, 1049, 1164
BstMAI GTCTC 7 cut(s) 131, 151, 273, 360, 601, 824, 884
BstMBI GATC 8 cut(s) 361, 556, 679, 765, 769, 942, 1046, 1161
BstMWI GCNNNNNNNGC 3 cut(s) 14, 349, 963
BstNI CCWGG 2 cut(s) 90, 810
BstSCI CCNGG 3 cut(s) 88, 808, 983
BstSFI CTRYAG 1 cut(s) 424
BstUI CGCG 1 cut(s) 251
BstV1I GCAGC 3 cut(s) 338, 352, 718
BstV2I GAAGAC 1 cut(s) 797
BstX2I RGATCY 2 cut(s) 556, 1046
BstXI CCANNNNNNTGG 1 cut(s) 923
BstYI RGATCY 2 cut(s) 556, 1046
Bsu15I ATCGAT 1 cut(s) 768
BsuRI GGCC 4 cut(s) 467, 583, 852, 1102
BsuTUI ATCGAT 1 cut(s) 768
BtrI CACGTC 1 cut(s) 1228
BtsCI GGATG 4 cut(s) 307, 392, 517, 533
BtsI GCAGTG 1 cut(s) 973
BtsIMutI CAGTG 3 cut(s) 672, 958, 973
Cac8I GCNNGC 2 cut(s) 846, 850
CaiI CAGNNNCTG 3 cut(s) 224, 286, 329
CfoI GCGC 2 cut(s) 253, 697
Cfr13I GGNCC 2 cut(s) 113, 465
ClaI ATCGAT 1 cut(s) 768
CseI GACGC 4 cut(s) 14, 35, 85, 377
Csp6I GTAC 3 cut(s) 530, 1033, 1262
CspCI CAANNNNNGTGG 2 cut(s) 934, 969
CviAII CATG 3 cut(s) 97, 508, 1238
CviQI GTAC 3 cut(s) 530, 1033, 1262
DdeI CTNAG 1 cut(s) 128
DpnI GATC 8 cut(s) 363, 558, 681, 767, 771, 944, 1048, 1163
DpnII GATC 8 cut(s) 361, 556, 679, 765, 769, 942, 1046, 1161
EaeI YGGCCR 1 cut(s) 581
Eam1104I CTCTTC 6 cut(s) 145, 468, 550, 755, 882, 1175
EarI CTCTTC 6 cut(s) 145, 468, 550, 755, 882, 1175
Eco130I CCWWGG 2 cut(s) 403, 468
Eco31I GGTCTC 1 cut(s) 151
Eco47I GGWCC 1 cut(s) 113
Eco57I CTGAAG 6 cut(s) 201, 242, 393, 459, 642, 779
Eco88I CYCGRG 1 cut(s) 154
EcoO109I RGGNCCY 1 cut(s) 465
EcoRII CCWGG 2 cut(s) 88, 808
EcoT14I CCWWGG 2 cut(s) 403, 468
EcoT22I ATGCAT 1 cut(s) 394
ErhI CCWWGG 2 cut(s) 403, 468
Esp3I CGTCTC 2 cut(s) 131, 360
FaeI CATG 3 cut(s) 100, 511, 1241
FalI AAGNNNNNCTT 2 cut(s) 1151, 1183
FaqI GGGAC 2 cut(s) 291, 1238
FatI CATG 3 cut(s) 96, 507, 1237
FblI GTMKAC 1 cut(s) 1242
Fnu4HI GCNGC 5 cut(s) 15, 254, 327, 341, 732
FokI GGATG 4 cut(s) 294, 379, 520, 524
Fsp4HI GCNGC 5 cut(s) 15, 254, 327, 341, 732
FspBI CTAG 3 cut(s) 590, 834, 1050
FspI TGCGCA 1 cut(s) 696
GlaI GCGC 2 cut(s) 252, 696
GluI GCNGC 5 cut(s) 15, 254, 327, 341, 732
HaeIII GGCC 4 cut(s) 467, 583, 852, 1102
HapII CCGG 2 cut(s) 111, 985
HgaI GACGC 4 cut(s) 14, 35, 85, 377
HhaI GCGC 2 cut(s) 253, 697
Hin1I GRCGYC 1 cut(s) 77
Hin1II CATG 3 cut(s) 100, 511, 1241
Hin6I GCGC 2 cut(s) 251, 695
HinP1I GCGC 2 cut(s) 251, 695
HindIII AAGCTT 2 cut(s) 199, 213
HinfI GANTC 7 cut(s) 242, 637, 643, 802, 1055, 1136, 1244
HpaII CCGG 2 cut(s) 111, 985
HphI GGTGA 3 cut(s) 239, 751, 839
Hpy166II GTNNAC 1 cut(s) 1243
Hpy188I TCNGA 7 cut(s) 58, 131, 195, 501, 642, 684, 820
Hpy188III TCNNGA 8 cut(s) 111, 154, 156, 365, 590, 757, 1050, 1159
Hpy8I GTNNAC 1 cut(s) 1243
Hpy99I CGWCG 1 cut(s) 79
HpyAV CCTTC 5 cut(s) 45, 456, 818, 1034, 1138
HpyCH4III ACNGT 2 cut(s) 86, 282
HpyCH4IV ACGT 4 cut(s) 921, 999, 1205, 1227
HpyCH4V TGCA 1 cut(s) 392
HpyF10VI GCNNNNNNNGC 3 cut(s) 14, 349, 963
HpyF3I CTNAG 1 cut(s) 128
HpySE526I ACGT 4 cut(s) 921, 999, 1205, 1227
Hsp92I GRCGYC 1 cut(s) 77
Hsp92II CATG 3 cut(s) 100, 511, 1241
HspAI GCGC 2 cut(s) 251, 695
Kpn2I TCCGGA 1 cut(s) 110
Kzo9I GATC 8 cut(s) 361, 556, 679, 765, 769, 942, 1046, 1161
LmnI GCTCC 2 cut(s) 113, 357
Lsp1109I GCAGC 3 cut(s) 338, 352, 718
LweI GCATC 4 cut(s) 220, 379, 401, 502
MaeI CTAG 3 cut(s) 590, 834, 1050
MaeII ACGT 4 cut(s) 921, 999, 1205, 1227
MaeIII GTNAC 3 cut(s) 288, 520, 1201
MalI GATC 8 cut(s) 363, 558, 681, 767, 771, 944, 1048, 1163
MboI GATC 8 cut(s) 361, 556, 679, 765, 769, 942, 1046, 1161
MflI RGATCY 2 cut(s) 556, 1046
MlsI TGGCCA 1 cut(s) 583
MluCI AATT 7 cut(s) 19, 102, 119, 149, 357, 432, 1068
MluNI TGGCCA 1 cut(s) 583
MlyI GAGTC 1 cut(s) 1238
MmeI TCCRAC 2 cut(s) 697, 1173
Mox20I TGGCCA 1 cut(s) 583
Mph1103I ATGCAT 1 cut(s) 394
MroI TCCGGA 1 cut(s) 110
MroXI GAANNNNTTC 1 cut(s) 1023
MscI TGGCCA 1 cut(s) 583
MseI TTAA 6 cut(s) 147, 186, 210, 444, 663, 989
Msp20I TGGCCA 1 cut(s) 583
MspA1I CMGCKG 1 cut(s) 329
MspI CCGG 2 cut(s) 111, 985
MspR9I CCNGG 3 cut(s) 90, 810, 985
MvaI CCWGG 2 cut(s) 90, 810
MvnI CGCG 1 cut(s) 251
MwoI GCNNNNNNNGC 3 cut(s) 14, 349, 963
NciI CCSGG 1 cut(s) 985
NdeII GATC 8 cut(s) 361, 556, 679, 765, 769, 942, 1046, 1161
NlaIII CATG 3 cut(s) 100, 511, 1241
NlaIV GGNNCC 1 cut(s) 558
NmeAIII GCCGAG 1 cut(s) 878
NmuCI GTSAC 2 cut(s) 288, 1201
NsbI TGCGCA 1 cut(s) 696
NsiI ATGCAT 1 cut(s) 394
PaeR7I CTCGAG 1 cut(s) 154
PdmI GAANNNNTTC 1 cut(s) 1023
PfeI GAWTC 6 cut(s) 242, 637, 643, 802, 1055, 1136
PkrI GCNGC 5 cut(s) 16, 255, 328, 342, 733
PleI GAGTC 1 cut(s) 1238
PpsI GAGTC 1 cut(s) 1238
Psp6I CCWGG 2 cut(s) 88, 808
PspGI CCWGG 2 cut(s) 88, 808
PspN4I GGNNCC 1 cut(s) 558
PspPI GGNCC 2 cut(s) 113, 465
PstNI CAGNNNCTG 3 cut(s) 224, 286, 329
PsuI RGATCY 2 cut(s) 556, 1046
PvuII CAGCTG 1 cut(s) 329
RsaI GTAC 3 cut(s) 531, 1034, 1263
RsaNI GTAC 3 cut(s) 530, 1033, 1262
SaqAI TTAA 6 cut(s) 147, 186, 210, 444, 663, 989
SatI GCNGC 5 cut(s) 15, 254, 327, 341, 732
Sau3AI GATC 8 cut(s) 361, 556, 679, 765, 769, 942, 1046, 1161
Sau96I GGNCC 2 cut(s) 113, 465
SchI GAGTC 1 cut(s) 1238
ScrFI CCNGG 3 cut(s) 90, 810, 985
SfaNI GCATC 4 cut(s) 220, 379, 401, 502
SfcI CTRYAG 1 cut(s) 424
Sfr274I CTCGAG 1 cut(s) 154
SinI GGWCC 1 cut(s) 113
SlaI CTCGAG 1 cut(s) 154
SmlI CTYRAG 4 cut(s) 154, 602, 785, 1246
SmoI CTYRAG 4 cut(s) 154, 602, 785, 1246
Sse9I AATT 7 cut(s) 19, 102, 119, 149, 357, 432, 1068
SsiI CCGC 6 cut(s) 14, 171, 254, 554, 670, 957
SspMI CTAG 3 cut(s) 590, 834, 1050
StyD4I CCNGG 3 cut(s) 88, 808, 983
StyI CCWWGG 2 cut(s) 403, 468
TaaI ACNGT 2 cut(s) 86, 282
TaiI ACGT 4 cut(s) 924, 1002, 1208, 1230
TaqI TCGA 6 cut(s) 74, 155, 364, 568, 768, 1142
TasI AATT 7 cut(s) 19, 102, 119, 149, 357, 432, 1068
TauI GCSGC 2 cut(s) 17, 256
TfiI GAWTC 6 cut(s) 242, 637, 643, 802, 1055, 1136
Tru1I TTAA 6 cut(s) 147, 186, 210, 444, 663, 989
Tru9I TTAA 6 cut(s) 147, 186, 210, 444, 663, 989
TscAI CASTG 3 cut(s) 679, 958, 973
TseFI GTSAC 2 cut(s) 288, 1201
TseI GCWGC 3 cut(s) 326, 340, 731
Tsp45I GTSAC 2 cut(s) 288, 1201
TspDTI ATGAA 6 cut(s) 85, 496, 537, 837, 891, 989
TspRI CASTG 3 cut(s) 679, 958, 973
VpaK11BI GGWCC 1 cut(s) 113
XapI RAATTY 2 cut(s) 19, 149
XbaI TCTAGA 2 cut(s) 589, 1049
XhoI CTCGAG 1 cut(s) 154
XmiI GTMKAC 1 cut(s) 1242
XmnI GAANNNNTTC 1 cut(s) 1023
XspI CTAG 3 cut(s) 590, 834, 1050
Zsp2I ATGCAT 1 cut(s) 394
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.