FvH4_5g17760

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0004076
Physical Location & Seq
Forward (+)
10167541 .. 10169791
2251 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g17760.t1

Sequence Viewer

Length: 1242 bp
ATGGAAGCAGCAGCCACGGCGAGCTTTGTCCACTCATCAGTCGCGGCCTTCAGGCCTTCCAATTCAGTCGGAAGGTCGCCTCTTCTGATGGGCCCCAAGTTTCTGCCTTTGGGACCTTTGGGTGCCAAATCTCACTCCTCCATCAAGCAGCATCAATTGGGTTTTGGAAGGAGGAGGTGCTTGGTTGTTAGGGCGGCGTCTTCGTCTTCAGACTCTGGTGGTTCTAGTGCTCCAATTGCTCCACTTCAGCTGGAGTCTCCGATTGGTCAGTTTTTATCTCAGATGATGATAAGCCACCCGCATCTTGTGCCTACTGCTGTCGAGCAGCAGCTGGAACAGCTCCAGACGGCCCGCGATGCTGAGGAAAAGAAGGAGAAGGAAGAGGCTTCCGCGTCGGGCACTGATCTTGTTTTATATAGGAGAATTGCCGAGGTTAAAGCTAATGAAAGGAAGAAGGCTCTCGAAGAGATATTATATGCGTTGGTGGTGCAGAAATTCATGGATGCTAATGTTTCTTTGGTACCCTCTATTGCCCCGGCTGCTTCAGACCCTTCTGGCCGAGTTGATGCATGGCCGAGTCAGGATGACAAGCTTGAGCGACTGCATTCTCCTGAAGCCTACGAGATGATTCAGAATCACCTGTCTCTGATCCTAGGGAATCGGTTGGACGACTCGACCTCTTTAGCGCAGATAAGCAAGCTCAAAGTGGGGCAGGTTTATGCGGCATCTGTAATGTATGGATACTTCCTCAAGCGGGTTGATCAGAGGTTTCAGCTTGAGAAGACAATGAAGATCCTTCCAGGTTCACTGGGCGGAGAAGACAGTGATATTGAAAAGGCAGTGGGTGAGGACAAGAGGCTTGGTGGTGTGGATAATTCAGCTTCCCATCCTGAAGTGTCTTCTTGGTCTGGGGGCTTGGGTTCTGGAGGTTTTGGTAACTTGATCAAACCATCTCGCTTGAGAACCTATGTTATGTCTGTTGATGGAGAGACCCTCCAAAGATATGCAACAATCAGATCAAGAGAGGCTGTTAGCATCATTGAAAAGCATACCGAGGCATTGTTTGGGAGACCTGATATTGTTATAACTCCTCAGGGGACAGTTGATTCTTCCAACGATGAACACATCAAAATCAGCTTTGGTGGTTTGAAGAGACTTGTTCTGGAAGCTGTGACTTTCGGTTCTTTCCTCTGGGATGTTGAGAGCTATGTGGATTCCAGGTACCATTTTGTTACAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000302 GO:0001101 GO:0006950 GO:0006979 GO:0008150 GO:0009314 GO:0009411 GO:0009416 GO:0009611 GO:0009628 GO:0010035 GO:0010193 GO:0010224 GO:0042221 GO:0042493 GO:0046677 GO:0050896 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF760

Protein Analysis

414

Amino Acids

45.0

Weight (kDa)

6.44

Isoelectric Point (pI)

49.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF760 PF05542 138 - 263 4.7e-21 Protein of unknown function (DUF760)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1085
Acc36I ACCTGC 1 cut(s) 703
Acc65I GGTACC 2 cut(s) 520, 1221
AccB1I GGYRCC 3 cut(s) 122, 520, 1221
AccII CGCG 3 cut(s) 44, 354, 392
AciI CCGC 8 cut(s) 44, 194, 299, 352, 390, 722, 754, 813
AclWI GGATC 2 cut(s) 643, 787
AcoI YGGCCR 2 cut(s) 556, 572
AcsI RAATTY 1 cut(s) 494
AcuI CTGAAG 6 cut(s) 34, 192, 230, 528, 633, 912
AcyI GRCGYC 1 cut(s) 197
AfaI GTAC 2 cut(s) 522, 1223
AfiI CCNNNNNNNGG 1 cut(s) 754
AgsI TTSAA 3 cut(s) 833, 1043, 1150
AjnI CCWGG 2 cut(s) 799, 1217
Alw21I GWGCWC 1 cut(s) 232
Alw26I GTCTC 5 cut(s) 261, 648, 983, 1063, 1147
AlwI GGATC 2 cut(s) 643, 787
AlwNI CAGNNNCTG 2 cut(s) 215, 331
AoxI GGCC 6 cut(s) 45, 53, 91, 348, 556, 572
ApaI GGGCCC 1 cut(s) 95
ApeKI GCWGC 6 cut(s) 8, 11, 148, 325, 328, 539
ApoI RAATTY 1 cut(s) 494
Asp718I GGTACC 2 cut(s) 520, 1221
AspA2I CCTAGG 1 cut(s) 652
AspLEI GCGC 1 cut(s) 688
AspS9I GGNCC 4 cut(s) 91, 92, 113, 349
AsuC2I CCSGG 1 cut(s) 536
AsuHPI GGTGA 2 cut(s) 629, 857
AvaII GGWCC 1 cut(s) 113
AvrII CCTAGG 1 cut(s) 652
AxyI CCTNAGG 1 cut(s) 1092
BaeGI GKGCMC 2 cut(s) 95, 401
BanI GGYRCC 3 cut(s) 122, 520, 1221
BanII GRGCYC 1 cut(s) 95
BarI GAAGNNNNNNTAC 2 cut(s) 728, 760
BbsI GAAGAC 5 cut(s) 192, 198, 788, 825, 891
Bbv12I GWGCWC 1 cut(s) 232
BbvCI CCTCAGC 1 cut(s) 360
BbvI GCAGC 6 cut(s) 20, 23, 160, 337, 340, 526
BccI CCATC 5 cut(s) 82, 149, 894, 958, 977
BceAI ACGGC 2 cut(s) 33, 363
BciT130I CCWGG 2 cut(s) 801, 1219
BciVI GTATCC 1 cut(s) 734
BclI TGATCA 2 cut(s) 760, 942
BcnI CCSGG 1 cut(s) 536
BcoDI GTCTC 5 cut(s) 261, 648, 983, 1063, 1147
BfaI CTAG 2 cut(s) 225, 653
BfuAI ACCTGC 1 cut(s) 703
BfuI GTATCC 1 cut(s) 734
BisI GCNGC 9 cut(s) 9, 12, 45, 149, 195, 326, 329, 540, 723
BlnI CCTAGG 1 cut(s) 652
BlsI GCNGC 9 cut(s) 10, 13, 46, 150, 196, 327, 330, 541, 724
Bme1390I CCNGG 3 cut(s) 536, 801, 1219
Bme18I GGWCC 1 cut(s) 113
BmgT120I GGNCC 4 cut(s) 91, 92, 113, 349
BmiI GGNNCC 6 cut(s) 93, 94, 114, 124, 522, 1223
BmrFI CCNGG 3 cut(s) 536, 801, 1219
BmrI ACTGGG 1 cut(s) 818
BmsI GCATC 7 cut(s) 160, 310, 346, 493, 556, 734, 1044
BmuI ACTGGG 1 cut(s) 818
BpiI GAAGAC 5 cut(s) 192, 198, 788, 825, 891
BplI GAGNNNNNCTC 2 cut(s) 978, 1010
BpmI CTGGAG 3 cut(s) 272, 326, 945
Bpu10I CCTNAGC 1 cut(s) 360
BpuEI CTTGAG 4 cut(s) 614, 734, 797, 979
BpuMI CCSGG 1 cut(s) 536
BsaHI GRCGYC 1 cut(s) 197
BsaI GGTCTC 2 cut(s) 983, 1063
BsaJI CCNNGG 5 cut(s) 15, 429, 534, 652, 1053
Bsc4I CCNNNNNNNGG 1 cut(s) 754
Bse1I ACTGG 1 cut(s) 813
Bse21I CCTNAGG 1 cut(s) 1092
BseBI CCWGG 2 cut(s) 801, 1219
BseDI CCNNGG 5 cut(s) 15, 429, 534, 652, 1053
BseGI GGATG 4 cut(s) 508, 589, 886, 1201
BseLI CCNNNNNNNGG 1 cut(s) 754
BseMII CTCAG 3 cut(s) 293, 351, 1106
BseNI ACTGG 1 cut(s) 813
BseRI GAGGAG 3 cut(s) 127, 187, 1080
BseSI GKGCMC 2 cut(s) 95, 401
BseXI GCAGC 6 cut(s) 20, 23, 160, 337, 340, 526
BsgI GTGCAG 1 cut(s) 509
Bsh1236I CGCG 3 cut(s) 44, 354, 392
BshFI GGCC 6 cut(s) 47, 55, 93, 350, 558, 574
BshNI GGYRCC 3 cut(s) 122, 520, 1221
BsiHKAI GWGCWC 1 cut(s) 232
BsiSI CCGG 1 cut(s) 536
BslFI GGGAC 2 cut(s) 126, 1111
BslI CCNNNNNNNGG 1 cut(s) 754
BsmAI GTCTC 5 cut(s) 261, 648, 983, 1063, 1147
BsmFI GGGAC 2 cut(s) 126, 1111
BsmI GAATGC 1 cut(s) 604
BsnI GGCC 6 cut(s) 47, 55, 93, 350, 558, 574
Bso31I GGTCTC 2 cut(s) 983, 1063
Bsp120I GGGCCC 1 cut(s) 91
Bsp1286I GDGCHC 3 cut(s) 95, 232, 401
Bsp143I GATC 6 cut(s) 403, 648, 760, 792, 942, 1016
BspACI CCGC 8 cut(s) 44, 194, 299, 352, 390, 722, 754, 813
BspANI GGCC 6 cut(s) 47, 55, 93, 350, 558, 574
BspCNI CTCAG 3 cut(s) 292, 352, 1105
BspFNI CGCG 3 cut(s) 44, 354, 392
BspLI GGNNCC 6 cut(s) 93, 94, 114, 124, 522, 1223
BspMI ACCTGC 1 cut(s) 703
BspPI GGATC 2 cut(s) 643, 787
BspT107I GGYRCC 3 cut(s) 122, 520, 1221
BspTNI GGTCTC 2 cut(s) 983, 1063
BsrI ACTGG 1 cut(s) 813
BssECI CCNNGG 5 cut(s) 15, 429, 534, 652, 1053
BssMI GATC 6 cut(s) 403, 648, 760, 792, 942, 1016
BssNI GRCGYC 1 cut(s) 197
BssT1I CCWWGG 1 cut(s) 652
Bst2UI CCWGG 2 cut(s) 801, 1219
Bst4CI ACNGT 2 cut(s) 824, 1102
Bst6I CTCTTC 4 cut(s) 87, 375, 459, 1145
BstACI GRCGYC 1 cut(s) 197
BstAPI GCANNNNNTGC 1 cut(s) 307
BstC8I GCNNGC 3 cut(s) 22, 352, 698
BstDEI CTNAG 3 cut(s) 279, 360, 1092
BstDSI CCRYGG 1 cut(s) 15
BstF5I GGATG 4 cut(s) 508, 589, 886, 1201
BstFNI CGCG 3 cut(s) 44, 354, 392
BstHHI GCGC 1 cut(s) 688
BstKTI GATC 6 cut(s) 406, 651, 763, 795, 945, 1019
BstMAI GTCTC 5 cut(s) 261, 648, 983, 1063, 1147
BstMBI GATC 6 cut(s) 403, 648, 760, 792, 942, 1016
BstMWI GCNNNNNNNGC 6 cut(s) 17, 236, 307, 337, 356, 539
BstNI CCWGG 2 cut(s) 801, 1219
BstSCI CCNGG 3 cut(s) 534, 799, 1217
BstSLI GKGCMC 2 cut(s) 95, 401
BstUI CGCG 3 cut(s) 44, 354, 392
BstV1I GCAGC 6 cut(s) 20, 23, 160, 337, 340, 526
BstV2I GAAGAC 5 cut(s) 192, 198, 788, 825, 891
BstX2I RGATCY 1 cut(s) 792
BstYI RGATCY 1 cut(s) 792
Bsu36I CCTNAGG 1 cut(s) 1092
BsuI GTATCC 1 cut(s) 734
BsuRI GGCC 6 cut(s) 47, 55, 93, 350, 558, 574
BtgI CCRYGG 1 cut(s) 15
BtgZI GCGATG 1 cut(s) 369
BtsCI GGATG 4 cut(s) 508, 589, 886, 1201
BtsI GCAGTG 1 cut(s) 846
BtsIMutI CAGTG 4 cut(s) 399, 806, 829, 846
BveI ACCTGC 1 cut(s) 703
Cac8I GCNNGC 3 cut(s) 22, 352, 698
CaiI CAGNNNCTG 2 cut(s) 215, 331
CfoI GCGC 1 cut(s) 688
Cfr13I GGNCC 4 cut(s) 91, 92, 113, 349
CseI GACGC 2 cut(s) 186, 381
Csp6I GTAC 2 cut(s) 521, 1222
CviAII CATG 2 cut(s) 499, 570
CviQI GTAC 2 cut(s) 521, 1222
DdeI CTNAG 3 cut(s) 279, 360, 1092
DpnI GATC 6 cut(s) 405, 650, 762, 794, 944, 1018
DpnII GATC 6 cut(s) 403, 648, 760, 792, 942, 1016
EaeI YGGCCR 2 cut(s) 556, 572
Eam1104I CTCTTC 4 cut(s) 87, 375, 459, 1145
EarI CTCTTC 4 cut(s) 87, 375, 459, 1145
EciI GGCGGA 1 cut(s) 828
Eco130I CCWWGG 1 cut(s) 652
Eco147I AGGCCT 1 cut(s) 55
Eco24I GRGCYC 1 cut(s) 95
Eco31I GGTCTC 2 cut(s) 983, 1063
Eco47I GGWCC 1 cut(s) 113
Eco57I CTGAAG 6 cut(s) 34, 192, 230, 528, 633, 912
Eco81I CCTNAGG 1 cut(s) 1092
EcoO109I RGGNCCY 2 cut(s) 92, 113
EcoRII CCWGG 2 cut(s) 799, 1217
EcoT14I CCWWGG 1 cut(s) 652
EcoT22I ATGCAT 1 cut(s) 571
EcoT38I GRGCYC 1 cut(s) 95
ErhI CCWWGG 1 cut(s) 652
FaeI CATG 2 cut(s) 502, 573
FaqI GGGAC 2 cut(s) 126, 1111
FatI CATG 2 cut(s) 498, 569
FauI CCCGC 3 cut(s) 306, 359, 747
FbaI TGATCA 2 cut(s) 760, 942
Fnu4HI GCNGC 9 cut(s) 9, 12, 45, 149, 195, 326, 329, 540, 723
FokI GGATG 4 cut(s) 515, 596, 873, 1208
FriOI GRGCYC 1 cut(s) 95
Fsp4HI GCNGC 9 cut(s) 9, 12, 45, 149, 195, 326, 329, 540, 723
FspBI CTAG 2 cut(s) 225, 653
GlaI GCGC 1 cut(s) 687
GluI GCNGC 9 cut(s) 9, 12, 45, 149, 195, 326, 329, 540, 723
GsuI CTGGAG 3 cut(s) 272, 326, 945
HaeIII GGCC 6 cut(s) 47, 55, 93, 350, 558, 574
HapII CCGG 1 cut(s) 536
HgaI GACGC 2 cut(s) 186, 381
HhaI GCGC 1 cut(s) 688
Hin1I GRCGYC 1 cut(s) 197
Hin1II CATG 2 cut(s) 502, 573
Hin6I GCGC 1 cut(s) 686
HinP1I GCGC 1 cut(s) 686
HindIII AAGCTT 1 cut(s) 590
HinfI GANTC 9 cut(s) 212, 254, 577, 628, 634, 658, 671, 1106, 1214
HpaII CCGG 1 cut(s) 536
HphI GGTGA 2 cut(s) 629, 857
Hpy166II GTNNAC 2 cut(s) 31, 806
Hpy188III TCNNGA 8 cut(s) 343, 461, 581, 611, 890, 924, 1020, 1163
Hpy8I GTNNAC 2 cut(s) 31, 806
Hpy99I CGWCG 1 cut(s) 397
HpyAV CCTTC 9 cut(s) 58, 66, 66, 162, 364, 370, 448, 561, 806
HpyCH4III ACNGT 2 cut(s) 824, 1102
HpyCH4V TGCA 4 cut(s) 490, 569, 604, 1007
HpyF10VI GCNNNNNNNGC 6 cut(s) 17, 236, 307, 337, 356, 539
HpyF3I CTNAG 3 cut(s) 279, 360, 1092
Hsp92I GRCGYC 1 cut(s) 197
Hsp92II CATG 2 cut(s) 502, 573
HspAI GCGC 1 cut(s) 686
KpnI GGTACC 2 cut(s) 524, 1225
Ksp22I TGATCA 2 cut(s) 760, 942
Kzo9I GATC 6 cut(s) 403, 648, 760, 792, 942, 1016
LmnI GCTCC 3 cut(s) 235, 244, 345
Lsp1109I GCAGC 6 cut(s) 20, 23, 160, 337, 340, 526
LweI GCATC 7 cut(s) 160, 310, 346, 493, 556, 734, 1044
MaeI CTAG 2 cut(s) 225, 653
MaeIII GTNAC 3 cut(s) 935, 1171, 1231
MalI GATC 6 cut(s) 405, 650, 762, 794, 944, 1018
MboI GATC 6 cut(s) 403, 648, 760, 792, 942, 1016
MfeI CAATTG 2 cut(s) 155, 234
MflI RGATCY 1 cut(s) 792
MhlI GDGCHC 3 cut(s) 95, 232, 401
MluCI AATT 6 cut(s) 61, 155, 234, 423, 494, 874
MlyI GAGTC 4 cut(s) 206, 263, 586, 665
MmeI TCCRAC 3 cut(s) 49, 645, 1137
Mph1103I ATGCAT 1 cut(s) 571
MseI TTAA 1 cut(s) 435
MspA1I CMGCKG 2 cut(s) 250, 331
MspI CCGG 1 cut(s) 536
MspR9I CCNGG 3 cut(s) 536, 801, 1219
MunI CAATTG 2 cut(s) 155, 234
Mva1269I GAATGC 1 cut(s) 604
MvaI CCWGG 2 cut(s) 801, 1219
MvnI CGCG 3 cut(s) 44, 354, 392
MwoI GCNNNNNNNGC 6 cut(s) 17, 236, 307, 337, 356, 539
NciI CCSGG 1 cut(s) 536
NdeII GATC 6 cut(s) 403, 648, 760, 792, 942, 1016
NlaIII CATG 2 cut(s) 502, 573
NlaIV GGNNCC 6 cut(s) 93, 94, 114, 124, 522, 1223
NmeAIII GCCGAG 3 cut(s) 454, 584, 600
NmuCI GTSAC 1 cut(s) 1171
NsiI ATGCAT 1 cut(s) 571
PceI AGGCCT 1 cut(s) 55
PctI GAATGC 1 cut(s) 604
PfeI GAWTC 5 cut(s) 628, 634, 658, 1106, 1214
PkrI GCNGC 9 cut(s) 10, 13, 46, 150, 196, 327, 330, 541, 724
PleI GAGTC 4 cut(s) 206, 262, 585, 665
PpsI GAGTC 4 cut(s) 206, 262, 585, 665
PpuMI RGGWCCY 1 cut(s) 113
PsiI TTATAA 1 cut(s) 1085
Psp5II RGGWCCY 1 cut(s) 113
Psp6I CCWGG 2 cut(s) 799, 1217
PspGI CCWGG 2 cut(s) 799, 1217
PspN4I GGNNCC 6 cut(s) 93, 94, 114, 124, 522, 1223
PspOMI GGGCCC 1 cut(s) 91
PspPI GGNCC 4 cut(s) 91, 92, 113, 349
PspPPI RGGWCCY 1 cut(s) 113
PstNI CAGNNNCTG 2 cut(s) 215, 331
PsuI RGATCY 1 cut(s) 792
PvuII CAGCTG 2 cut(s) 250, 331
RsaI GTAC 2 cut(s) 522, 1223
RsaNI GTAC 2 cut(s) 521, 1222
SaqAI TTAA 1 cut(s) 435
SatI GCNGC 9 cut(s) 9, 12, 45, 149, 195, 326, 329, 540, 723
Sau3AI GATC 6 cut(s) 403, 648, 760, 792, 942, 1016
Sau96I GGNCC 4 cut(s) 91, 92, 113, 349
SchI GAGTC 4 cut(s) 206, 263, 586, 665
ScrFI CCNGG 3 cut(s) 536, 801, 1219
SduI GDGCHC 3 cut(s) 95, 232, 401
SfaNI GCATC 7 cut(s) 160, 310, 346, 493, 556, 734, 1044
SinI GGWCC 1 cut(s) 113
SmlI CTYRAG 4 cut(s) 593, 749, 776, 958
SmoI CTYRAG 4 cut(s) 593, 749, 776, 958
Sse9I AATT 6 cut(s) 61, 155, 234, 423, 494, 874
SseBI AGGCCT 1 cut(s) 55
SsiI CCGC 8 cut(s) 44, 194, 299, 352, 390, 722, 754, 813
SspMI CTAG 2 cut(s) 225, 653
StuI AGGCCT 1 cut(s) 55
StyD4I CCNGG 3 cut(s) 534, 799, 1217
StyI CCWWGG 1 cut(s) 652
TaaI ACNGT 2 cut(s) 824, 1102
TaqI TCGA 3 cut(s) 321, 462, 674
TasI AATT 6 cut(s) 61, 155, 234, 423, 494, 874
TauI GCSGC 3 cut(s) 47, 197, 725
TfiI GAWTC 5 cut(s) 628, 634, 658, 1106, 1214
Tru1I TTAA 1 cut(s) 435
Tru9I TTAA 1 cut(s) 435
TscAI CASTG 4 cut(s) 406, 813, 829, 846
TseFI GTSAC 1 cut(s) 1171
TseI GCWGC 6 cut(s) 8, 11, 148, 325, 328, 539
Tsp45I GTSAC 1 cut(s) 1171
TspDTI ATGAA 4 cut(s) 459, 487, 803, 1134
TspRI CASTG 4 cut(s) 406, 813, 829, 846
VpaK11BI GGWCC 1 cut(s) 113
XapI RAATTY 1 cut(s) 494
XmaJI CCTAGG 1 cut(s) 652
XspI CTAG 2 cut(s) 225, 653
Zsp2I ATGCAT 1 cut(s) 571
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.