AT1G48650

RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0013882
Physical Location & Seq
Reverse (-)
17989131 .. 17995339
6209 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G48650.2

Sequence Viewer

Length: 3594 bp
ATGCAACTTTACTCTGCAATTTTCCGAGGACATATCAGACCTAACGCCGTCGTCGGTGCTGCCTCCATGTTTATTCAACATAATTCCATCCAGCTTCATCGGAGCCCTAAATTGTTGTTGAGACCTTCCTCTGTAGTAAGGTCTCTTCATTGCCGGAGGTCAGGTGGATTGGTTACGCATTCTCAGAGGTCAAGGGTCTTGTGTGTGAAAGCAGCTCGTGGTGATGCTTCTTCTTCGACTCTTGGAATAGAGTGGAGAGCAGCTAATTTACCATATTTTCAGAGGCAAAACTCTGGCTATGGACGTATTGCTTACAATGACTATGAATCAAGTGATGAGTCGGATAGAGATGTTGGGTCCTCTCAGTCTCAGCAAATGGCGGGTTCGACTCTTGATAACATTGATCAATGGAGATTTAAACTAACTATGCTTCTCCGGAACAAAGAAGACCAAGAAGTTGTTTCAAGGGAGAGAAAAGATCGGCGTGATTTTGATCATATCTCTGCTTTGGCAACTAGGATGGGATTACACAGCCGTCAGTATTCGAAGATTGTTGTCATTAGTAAAGCTCCTTTGCCAAATTATAGACCTGATCTTGATGATAAACGCCCACAAAGAGAGGTGGTTTTGCCTTTTGGTCTACAAAGCGAAGTAGATGCCCATCTACATTCTTTCCTCGATCAGAAAAAAACATTGATTCCGGAGATGCCCAGGCAAAATAGTAGTGAAAGTTTAGCAAACGGTTATGGAAACTATGAGACACCAGAGACCGTGATGCAAAATAGTCTTGCTAGGGAAAGAATACTCCGCCCAAGAAGCTTACAACTGAAGAGCAAGCAACAGCAATGGGTGGATTCTCCAGAAGGCCAGAAAATGGTAGGGTTTCGCAAAACCCTTCCTGCTTACAAAGAGAAAGATGCACTACTGAAAGCCATTGCAGCAAACCAGGTCGTAGTGGTTTCTGGAGAAACTGGATGTGGTAAAACAACACAGCTTCCTCAGTACATATTAGAATCTGAGATCGAGGCTGCCCGTGGAGCTACATGCAGCATCATATGTACCCAGCCTAGAAGAATATCGGCTATTTCTGTTTCTGAACGAGTTGCTGCAGAACGTGGAGAACAAATTGGAGAATCTGTTGGCTACAAAGTGCGGCTTGAAGGAATGAGAGGGAGAGATACACGTCTTCTTTTTTGTACAACTGGTGTTTTACTTAGAAGGTTACTTGTGGATCGTAGCCTGAAAGGTGTGACTCATGTCGTAGTTGATGAGATTCATGAACGTGGAATGAATGAAGATTTCCTGCTTATTGTCCTGAAAGATCTTCTTCCTCGTCGACCTGACCTAAAGTTGATTTTAATGAGTGCTACTTTGAACGCTGAGCTCTTTTCTTCTTATTTTGGTGGTGCACCAGCCATGCATATCCCAGGTTTTACATACCCTGTCCGAGCCCATTTCTTGGAGGATTATCTTGAGACGAGTGGGTACAGGCTGACAACATATAATCAAATTGATGATTATGGTGAGGAAAAGACATGGAAGATGCAAAAACAAGCTCAGTTCAAAAAAAGAAAATCCCTAATATCCTCTGCAGTAGAGGATGCTCTTGAAGCTGCAGACTTCAAAGGATACAACTTTCGTACTAGAGATTCTTTGTCATGCTGGAGTCCTGATTCAATAGGCTTTAACCTCATTGAAAATGTACTCTGTCACATCGTTAAAGGAGAGAGACCTGGTGCTGTTTTGGTATTCATGACCGGTTGGGACGATATAAACTCCTTGAAGAATCAGCTTGAAGCTCATTCTCTTCTAGGAGACCCGAACAAAGTTTTGTTACTTGCCTGTCATGGATCCATGGCCAGCTCTGAGCAGAGGCTGATTTTTGATAGACCTCCAGAAGGAATTAGGAAAATAGTACTGGCCACCAATATGGCTGAGACAAGTATCACCATCAATGATGTTGTATATGTGATTGATTGTGGGAAAGCAAAAGAAACATCTTATGATGCGCTTAACAATACCCCTTGTCTGCTTCCATCTTGGATTTCAAAGGCAGCTGCTCGCCAAAGAAGAGGAAGAGCTGGTCGTGTTATGCCTGGAGAATGTTATCACCTCTATCCTAGATGTGTCTACGAAGCTTTTGCTGACTACCAGCAGCCGGAACTTCTAAGGACGCCGTTGCAGTCTCTGTGTTTACAAATTAAAAGCCTAGGACTTGGAAGCATTTCGGAGTTCCTCTCCAGGGCATTGCAACCTCCTGAAGCATTATCGGTCCAAAATGCTGTTGAGTATCTAAAAATCATTGGGGCTCTTGATGACGATGAAAATTTAACACCTTTAGGGAAAAATCTGTCAATGCTTCCTGTGGAGCCTAAACTTGGAAAAATGCTTATTTTAGGGGCTATCTTCAACTGTCTAGATCCAGTAATGACAGTTGTTGCTGGTCTTAGTGTCAGAGACCCATTCCTTATGCCATTTGACAAAAAAGATCTAGCAGAAACAGCAAGGTCCAAGTTCTCTGGCCGTGATTACAGTGATCATTTGACATTGGTTCGAGCATACAATGGTTGGAAAGATGCTGAAAGAACACATTCTGGTTACGACTATTGCTGGAAGAACTTTCTCTCTTCTCAAACTCTTAAGGCCATGGACTCTATGCGGAAACAATTCTTCAATCTCCTCAAGGAGGCTTCTCTGATTGATAACATTGAAGGTTGCAGTAAGCTGAGTCATGATGAACATCTTGTACGCGCAATCATTTGTGCTGGCATGTTCCCTGGAGTCTGTTCTGTTGTTAATAAGGAAAAGTCAATTACGCTCAAGACAATGGAAGATGGACAAGTGCTTCTATACTCGAGTTCTGTGAATGGCAATGTACCAATGATTCCCTTCCCTTGGCTAGTCTTCAACGACAAAGTGAAAGTTAATTCTGTGTTTCTGCGAGACTCTACTGCTGTATCTGACTCTGTTCTTCTTTTGTTTGGAGATAAAATCTCTTCCGGTGGATTTGATGGTCATCTCAAAATGCTTGGAGGATATCTGGAGTTTTTCATGAAACCTACTTTAGCATATACATACCTTTCGTTAAAAAGAGAGCTCGACGAACTTATTCAGAATAAGCTTGTGAATCCAAAACTTGATATTCAACTATACGACAAACTTATGACTGCCATTAGATTACTTGTGTCAGAGGACCAATGTGAGGGCAGATTCGTCTATGGCCGGAAAGCTCTAAGCCCTACACCAGCAAAGAAGTTAAAAGATGTAGGGGCTCAGCTTCAGAACAGCGGTGGTGAAAACAACAAGAACCAACTTCAGACATTACTTGCCCGAGCAGGACACGGATCACCTGTGTACAAGACAAGACAGCTTAAGAACAACCAGTTCCGCTCTATGGTTACCTTCAATGGATTGGATTTCATGGGAAAGCCATGTGGTAGTAAGAAAAATGCAGAGAAGGATGCAGCTCATGAGGCTTTGTTGTGGTTACAAGGCGAGTCCAAGTCAAGTCTGAATGATCTTAACCACATGTCAATGCTCTTAAAGAAGAACAAAAGTAAAAACCACGCCAAGGCATCAACTAAATGGGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001047 GO:0001067 GO:0001503 GO:0001817 GO:0001819 GO:0002151 GO:0002791 GO:0002793 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003697 GO:0003723 GO:0003724 GO:0003725 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006355 GO:0006357 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0008186 GO:0009605 GO:0009607 GO:0009615 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010501 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010638 GO:0014070 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0019899 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031330 GO:0032204 GO:0032206 GO:0032479 GO:0032481 GO:0032501 GO:0032647 GO:0032727 GO:0032879 GO:0032880 GO:0033043 GO:0033044 GO:0034641 GO:0042221 GO:0042623 GO:0042826 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043330 GO:0043331 GO:0043487 GO:0043489 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050707 GO:0050708 GO:0050714 GO:0050715 GO:0050789 GO:0050794 GO:0050896 GO:0051046 GO:0051047 GO:0051049 GO:0051050 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051222 GO:0051223 GO:0051239 GO:0051240 GO:0051252 GO:0051253 GO:0051254 GO:0051704 GO:0051707 GO:0051880 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070035 GO:0070201 GO:0071704 GO:0080090 GO:0090087 GO:0090304 GO:0090669 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1902369 GO:1902680 GO:1902739 GO:1902741 GO:1903506 GO:1903508 GO:1903530 GO:1903532 GO:1904951 GO:1990837 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind dsrm

Protein Analysis

1197

Amino Acids

134.25

Weight (kDa)

9.1

Isoelectric Point (pI)

42.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 309 - 461 5.6e-10 DEAD/DEAH box helicase
Helicase_C PF00271 567 - 697 1.5e-12 Helicase conserved C-terminal domain
WHD_HA2 PF04408 761 - 789 4.5e-09 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 790 - 850 1.2e-15 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 916 - 998 1.3e-18 Oligonucleotide/oligosaccharide-binding (OB)-fold
RNA_hel_CTD PF26026 1006 - 1064 1.3e-20 C-terminal domain in RNA helicases
dsrm PF00035 1102 - 1163 1e-06 Double-stranded RNA binding motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013882)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G48650 AT1G48650
fragaria_vesca FvH4_5g16860
malus_domestica MD06G1222500.v1.1 MD14G1231600.v1.1
prunus_persica Prupe.5G227100_v2.0.a1
pyrus_communis pycom06g19810 pycom06g19820 pycom14g19280
rosa_chinensis RchiOBHm_Chr7g0179211
rosa_laevigata RLG00000005374
rosa_multiflora Rmu_sc0009798.1_g000005 Rmu_sc0021799.1_g000001
rosa_roxburghii Rroxscaffold_3G00274220
rosa_rugosa Rorug06G0422200
rosa_samantha Rh7BG021500
rosa_wichuraiana Rw7G001770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 874, 1459
AccBSI CCGCTC 1 cut(s) 3390
AccI GTMKAC 3 cut(s) 640, 1336, 2130
AccII CGCG 1 cut(s) 2751
AccIII TCCGGA 2 cut(s) 435, 700
AciI CCGC 6 cut(s) 380, 808, 1153, 2659, 3288, 3388
AclWI GGATC 5 cut(s) 1239, 1845, 1858, 2414, 3352
AcoI YGGCCR 4 cut(s) 1857, 1920, 2521, 3220
AcsI RAATTY 1 cut(s) 2326
AcuI CTGAAG 4 cut(s) 848, 2280, 3263, 3299
AcyI GRCGYC 1 cut(s) 2174
AflII CTTAAG 2 cut(s) 2639, 3371
AflIII ACRYGT 2 cut(s) 1181, 3528
AgeI ACCGGT 1 cut(s) 1757
AjiI CACGTC 1 cut(s) 1184
AjnI CCWGG 7 cut(s) 710, 945, 1426, 1732, 2095, 2240, 2776
Alw21I GWGCWC 3 cut(s) 1386, 1411, 3099
Alw44I GTGCAC 1 cut(s) 1407
AlwI GGATC 5 cut(s) 1239, 1845, 1858, 2414, 3352
AlwNI CAGNNNCTG 2 cut(s) 1876, 2188
Ama87I CYCGRG 2 cut(s) 2854, 3330
Aor13HI TCCGGA 2 cut(s) 435, 700
AoxI GGCC 6 cut(s) 865, 1857, 1920, 2521, 2643, 3220
ApaLI GTGCAC 1 cut(s) 1407
ApoI RAATTY 1 cut(s) 2326
AsiGI ACCGGT 1 cut(s) 1757
Asp700I GAANNNNTTC 4 cut(s) 2224, 2590, 2666, 3108
AspA2I CCTAGG 1 cut(s) 2209
AspLEI GCGC 2 cut(s) 2011, 2753
AspS9I GGNCC 4 cut(s) 357, 2272, 2508, 3193
AsuHPI GGTGA 6 cut(s) 233, 1535, 1939, 2102, 3305, 3339
AsuII TTCGAA 1 cut(s) 545
AvaI CYCGRG 2 cut(s) 2854, 3330
AvaII GGWCC 4 cut(s) 357, 2272, 2508, 3193
AvrII CCTAGG 1 cut(s) 2209
BaeGI GKGCMC 1 cut(s) 1411
BaeI ACNNNNGTAYC 2 cut(s) 2940, 2973
BalI TGGCCA 2 cut(s) 1859, 1922
BamHI GGATCC 1 cut(s) 1850
BanII GRGCYC 6 cut(s) 107, 1386, 1453, 2311, 3099, 3274
BarI GAAGNNNNNNTAC 2 cut(s) 129, 161
BauI CACGAG 1 cut(s) 216
BbsI GAAGAC 3 cut(s) 453, 1178, 2896
Bbv12I GWGCWC 3 cut(s) 1386, 1411, 3099
BccI CCATC 7 cut(s) 95, 514, 669, 1958, 2044, 2828, 3005
BceAI ACGGC 4 cut(s) 32, 519, 2161, 2508
BcgI CGANNNNNNTGC 4 cut(s) 638, 672, 2123, 2157
BciT130I CCWGG 7 cut(s) 712, 947, 1428, 1734, 2097, 2242, 2778
BciVI GTATCC 1 cut(s) 1622
BclI TGATCA 3 cut(s) 403, 493, 2536
BfmI CTRYAG 4 cut(s) 132, 1107, 1590, 1614
BfrI CTTAAG 2 cut(s) 2639, 3371
BfuI GTATCC 1 cut(s) 1622
BglII AGATCT 2 cut(s) 1321, 2488
BlnI CCTAGG 1 cut(s) 2209
BlpI GCTNAGC 2 cut(s) 1380, 3273
BmcAI AGTACT 1 cut(s) 1917
Bme1390I CCNGG 7 cut(s) 712, 947, 1428, 1734, 2097, 2242, 2778
Bme18I GGWCC 4 cut(s) 357, 2272, 2508, 3193
BmeT110I CYCGRG 2 cut(s) 2854, 3330
BmgBI CACGTC 1 cut(s) 1184
BmgT120I GGNCC 4 cut(s) 357, 2272, 2508, 3193
BmiI GGNNCC 4 cut(s) 104, 358, 1852, 2370
BmrFI CCNGG 7 cut(s) 712, 947, 1428, 1734, 2097, 2242, 2778
BoxI GACNNNNGTC 1 cut(s) 1256
BpiI GAAGAC 3 cut(s) 453, 1178, 2896
BplI GAGNNNNNCTC 2 cut(s) 2222, 2254
BpmI CTGGAG 8 cut(s) 843, 984, 1684, 1878, 2118, 2224, 2799, 3062
Bpu1102I GCTNAGC 2 cut(s) 1380, 3273
Bpu14I TTCGAA 1 cut(s) 545
BpuEI CTTGAG 3 cut(s) 1493, 2666, 2804
BsaBI GATNNNNATC 4 cut(s) 492, 660, 2739, 3015
BsaHI GRCGYC 1 cut(s) 2174
BsaI GGTCTC 6 cut(s) 115, 147, 761, 1723, 1809, 2451
BsaWI WCCGGW 4 cut(s) 435, 700, 1757, 2999
BsaXI ACNNNNNCTCC 4 cut(s) 148, 178, 1122, 1152
Bse118I RCCGGY 1 cut(s) 1757
Bse1I ACTGG 5 cut(s) 976, 1207, 1923, 2423, 3382
Bse3DI GCAATG 5 cut(s) 148, 851, 933, 2246, 2878
Bse8I GATNNNNATC 4 cut(s) 492, 660, 2739, 3015
BseAI TCCGGA 2 cut(s) 435, 700
BseBI CCWGG 7 cut(s) 712, 947, 1428, 1734, 2097, 2242, 2778
BseGI GGATG 5 cut(s) 87, 525, 980, 1606, 3466
BseJI GATNNNNATC 4 cut(s) 492, 660, 2739, 3015
BseMI GCAATG 5 cut(s) 148, 851, 933, 2246, 2878
BseNI ACTGG 5 cut(s) 976, 1207, 1923, 2423, 3382
BseRI GAGGAG 1 cut(s) 2669
BseSI GKGCMC 1 cut(s) 1411
BseYI CCCAGC 1 cut(s) 1062
Bsh1236I CGCG 1 cut(s) 2751
BshFI GGCC 6 cut(s) 867, 1859, 1922, 2523, 2645, 3222
BshTI ACCGGT 1 cut(s) 1757
BsiHKAI GWGCWC 3 cut(s) 1386, 1411, 3099
BsiHKCI CYCGRG 2 cut(s) 2854, 3330
BsiSI CCGG 7 cut(s) 154, 436, 701, 1758, 2159, 3000, 3223
BslFI GGGAC 1 cut(s) 1778
BsmBI CGTCTC 1 cut(s) 1469
BsmFI GGGAC 1 cut(s) 1778
BsmI GAATGC 1 cut(s) 178
BsnI GGCC 6 cut(s) 867, 1859, 1922, 2523, 2645, 3222
Bso31I GGTCTC 6 cut(s) 115, 147, 761, 1723, 1809, 2451
BsoBI CYCGRG 2 cut(s) 2854, 3330
Bsp119I TTCGAA 1 cut(s) 545
Bsp1286I GDGCHC 7 cut(s) 107, 1386, 1411, 1453, 2311, 3099, 3274
Bsp13I TCCGGA 2 cut(s) 435, 700
Bsp1407I TGTACA 2 cut(s) 1196, 3354
Bsp1720I GCTNAGC 2 cut(s) 1380, 3273
Bsp19I CCATGG 2 cut(s) 1854, 2646
BspACI CCGC 6 cut(s) 380, 808, 1153, 2659, 3288, 3388
BspANI GGCC 6 cut(s) 867, 1859, 1922, 2523, 2645, 3222
BspEI TCCGGA 2 cut(s) 435, 700
BspFNI CGCG 1 cut(s) 2751
BspHI TCATGA 5 cut(s) 1276, 1752, 2731, 3051, 3469
BspLI GGNNCC 4 cut(s) 104, 358, 1852, 2370
BspMAI CTGCAG 3 cut(s) 1111, 1594, 1618
BspPI GGATC 5 cut(s) 1239, 1845, 1858, 2414, 3352
BspQI GCTCTTC 2 cut(s) 824, 2071
BspT104I TTCGAA 1 cut(s) 545
BspTI CTTAAG 2 cut(s) 2639, 3371
BspTNI GGTCTC 6 cut(s) 115, 147, 761, 1723, 1809, 2451
BsrBI CCGCTC 1 cut(s) 3390
BsrDI GCAATG 5 cut(s) 148, 851, 933, 2246, 2878
BsrFI RCCGGY 1 cut(s) 1757
BsrGI TGTACA 2 cut(s) 1196, 3354
BsrI ACTGG 5 cut(s) 976, 1207, 1923, 2423, 3382
BssAI RCCGGY 1 cut(s) 1757
BssNI GRCGYC 1 cut(s) 2174
BssSI CACGAG 1 cut(s) 216
BssT1I CCWWGG 5 cut(s) 1854, 2209, 2646, 2894, 3570
Bst2BI CACGAG 1 cut(s) 216
Bst2UI CCWGG 7 cut(s) 712, 947, 1428, 1734, 2097, 2242, 2778
Bst4CI ACNGT 5 cut(s) 743, 772, 2414, 2434, 2534
Bst6I CTCTTC 7 cut(s) 150, 824, 1812, 2065, 2071, 2632, 3001
BstACI GRCGYC 1 cut(s) 2174
BstAFI CTTAAG 2 cut(s) 2639, 3371
BstAUI TGTACA 2 cut(s) 1196, 3354
BstBI TTCGAA 1 cut(s) 545
BstC8I GCNNGC 4 cut(s) 836, 1861, 2062, 2767
BstDSI CCRYGG 3 cut(s) 1033, 1854, 2646
BstEII GGTNACC 1 cut(s) 3397
BstENI CCTNNNNNAGG 2 cut(s) 1896, 2684
BstF5I GGATG 5 cut(s) 87, 525, 980, 1606, 3466
BstFNI CGCG 1 cut(s) 2751
BstHHI GCGC 2 cut(s) 2011, 2753
BstMWI GCNNNNNNNGC 6 cut(s) 816, 938, 1037, 1610, 2501, 3473
BstNI CCWGG 7 cut(s) 712, 947, 1428, 1734, 2097, 2242, 2778
BstNSI RCATGY 3 cut(s) 1047, 2773, 3532
BstPAI GACNNNNGTC 1 cut(s) 1256
BstPI GGTNACC 1 cut(s) 3397
BstSCI CCNGG 7 cut(s) 710, 945, 1426, 1732, 2095, 2240, 2776
BstSFI CTRYAG 4 cut(s) 132, 1107, 1590, 1614
BstSLI GKGCMC 1 cut(s) 1411
BstUI CGCG 1 cut(s) 2751
BstV2I GAAGAC 3 cut(s) 453, 1178, 2896
BstX2I RGATCY 4 cut(s) 1321, 1850, 2419, 2488
BstXI CCANNNNNNTGG 1 cut(s) 1930
BstYI RGATCY 4 cut(s) 1321, 1850, 2419, 2488
BsuI GTATCC 1 cut(s) 1622
BsuRI GGCC 6 cut(s) 867, 1859, 1922, 2523, 2645, 3222
BtgI CCRYGG 3 cut(s) 1033, 1854, 2646
BtrI CACGTC 1 cut(s) 1184
BtsCI GGATG 5 cut(s) 87, 525, 980, 1606, 3466
BtsIMutI CAGTG 1 cut(s) 2539
Cac8I GCNNGC 4 cut(s) 836, 1861, 2062, 2767
CaiI CAGNNNCTG 2 cut(s) 1876, 2188
CciI TCATGA 5 cut(s) 1276, 1752, 2731, 3051, 3469
CfoI GCGC 2 cut(s) 2011, 2753
Cfr10I RCCGGY 1 cut(s) 1757
Cfr13I GGNCC 4 cut(s) 357, 2272, 2508, 3193
CseI GACGC 1 cut(s) 2182
CsiI ACCWGGT 2 cut(s) 945, 1732
CspAI ACCGGT 1 cut(s) 1757
DraI TTTAAA 1 cut(s) 418
EaeI YGGCCR 4 cut(s) 1857, 1920, 2521, 3220
Eam1104I CTCTTC 7 cut(s) 150, 824, 1812, 2065, 2071, 2632, 3001
EarI CTCTTC 7 cut(s) 150, 824, 1812, 2065, 2071, 2632, 3001
EciI GGCGGA 1 cut(s) 797
Ecl136II GAGCTC 2 cut(s) 1384, 3097
Eco130I CCWWGG 5 cut(s) 1854, 2209, 2646, 2894, 3570
Eco24I GRGCYC 6 cut(s) 107, 1386, 1453, 2311, 3099, 3274
Eco31I GGTCTC 6 cut(s) 115, 147, 761, 1723, 1809, 2451
Eco32I GATATC 1 cut(s) 3038
Eco47I GGWCC 4 cut(s) 357, 2272, 2508, 3193
Eco53kI GAGCTC 2 cut(s) 1384, 3097
Eco57I CTGAAG 4 cut(s) 848, 2280, 3263, 3299
Eco88I CYCGRG 2 cut(s) 2854, 3330
Eco91I GGTNACC 1 cut(s) 3397
EcoICRI GAGCTC 2 cut(s) 1384, 3097
EcoNI CCTNNNNNAGG 2 cut(s) 1896, 2684
EcoO109I RGGNCCY 1 cut(s) 357
EcoO65I GGTNACC 1 cut(s) 3397
EcoRII CCWGG 7 cut(s) 710, 945, 1426, 1732, 2095, 2240, 2776
EcoRV GATATC 1 cut(s) 3038
EcoT14I CCWWGG 5 cut(s) 1854, 2209, 2646, 2894, 3570
EcoT22I ATGCAT 1 cut(s) 1422
EcoT38I GRGCYC 6 cut(s) 107, 1386, 1453, 2311, 3099, 3274
ErhI CCWWGG 5 cut(s) 1854, 2209, 2646, 2894, 3570
Esp3I CGTCTC 1 cut(s) 1469
FalI AAGNNNNNCTT 4 cut(s) 1140, 1172, 1311, 1343
FaqI GGGAC 1 cut(s) 1778
FauI CCCGC 1 cut(s) 373
FauNDI CATATG 1 cut(s) 1055
FbaI TGATCA 3 cut(s) 403, 493, 2536
FblI GTMKAC 3 cut(s) 640, 1336, 2130
FokI GGATG 5 cut(s) 74, 532, 987, 1613, 3473
FriOI GRGCYC 6 cut(s) 107, 1386, 1453, 2311, 3099, 3274
GlaI GCGC 2 cut(s) 2010, 2752
GsaI CCCAGC 1 cut(s) 1066
GsuI CTGGAG 8 cut(s) 843, 984, 1684, 1878, 2118, 2224, 2799, 3062
HaeIII GGCC 6 cut(s) 867, 1859, 1922, 2523, 2645, 3222
HapII CCGG 7 cut(s) 154, 436, 701, 1758, 2159, 3000, 3223
HgaI GACGC 1 cut(s) 2182
HhaI GCGC 2 cut(s) 2011, 2753
Hin1I GRCGYC 1 cut(s) 2174
Hin6I GCGC 2 cut(s) 2009, 2751
HinP1I GCGC 2 cut(s) 2009, 2751
HincII GTYRAC 1 cut(s) 1337
HindII GTYRAC 1 cut(s) 1337
HindIII AAGCTT 3 cut(s) 817, 2136, 3119
HpaII CCGG 7 cut(s) 154, 436, 701, 1758, 2159, 3000, 3223
HphI GGTGA 6 cut(s) 233, 1535, 1939, 2102, 3305, 3339
Hpy166II GTNNAC 6 cut(s) 641, 1337, 1409, 2131, 2195, 3355
Hpy8I GTNNAC 6 cut(s) 641, 1337, 1409, 2131, 2195, 3355
Hpy99I CGWCG 4 cut(s) 53, 56, 1338, 3104
HpyCH4III ACNGT 5 cut(s) 743, 772, 2414, 2434, 2534
HpyCH4IV ACGT 4 cut(s) 304, 1114, 1183, 1282
HpyF10VI GCNNNNNNNGC 6 cut(s) 816, 938, 1037, 1610, 2501, 3473
HpySE526I ACGT 4 cut(s) 304, 1114, 1183, 1282
Hsp92I GRCGYC 1 cut(s) 2174
HspAI GCGC 2 cut(s) 2009, 2751
Kpn2I TCCGGA 2 cut(s) 435, 700
Ksp22I TGATCA 3 cut(s) 403, 493, 2536
LguI GCTCTTC 2 cut(s) 824, 2071
LmnI GCTCC 4 cut(s) 102, 574, 1037, 2368
MabI ACCWGGT 2 cut(s) 945, 1732
MaeII ACGT 4 cut(s) 304, 1114, 1183, 1282
MaeIII GTNAC 8 cut(s) 172, 1221, 1249, 1709, 1833, 2597, 3397, 3486
MbiI CCGCTC 1 cut(s) 3390
MflI RGATCY 4 cut(s) 1321, 1850, 2419, 2488
MhlI GDGCHC 7 cut(s) 107, 1386, 1411, 1453, 2311, 3099, 3274
MlsI TGGCCA 2 cut(s) 1859, 1922
MluNI TGGCCA 2 cut(s) 1859, 1922
MmeI TCCRAC 2 cut(s) 321, 2549
Mox20I TGGCCA 2 cut(s) 1859, 1922
Mph1103I ATGCAT 1 cut(s) 1422
MroI TCCGGA 2 cut(s) 435, 700
MroXI GAANNNNTTC 4 cut(s) 2224, 2590, 2666, 3108
MscI TGGCCA 2 cut(s) 1859, 1922
MslI CAYNNNNRTG 3 cut(s) 1281, 1928, 3533
Msp20I TGGCCA 2 cut(s) 1859, 1922
MspA1I CMGCKG 2 cut(s) 2057, 3288
MspCI CTTAAG 2 cut(s) 2639, 3371
MspI CCGG 7 cut(s) 154, 436, 701, 1758, 2159, 3000, 3223
MspR9I CCNGG 7 cut(s) 712, 947, 1428, 1734, 2097, 2242, 2778
Mva1269I GAATGC 1 cut(s) 178
MvaI CCWGG 7 cut(s) 712, 947, 1428, 1734, 2097, 2242, 2778
MvnI CGCG 1 cut(s) 2751
MwoI GCNNNNNNNGC 6 cut(s) 816, 938, 1037, 1610, 2501, 3473
NcoI CCATGG 2 cut(s) 1854, 2646
NdeI CATATG 1 cut(s) 1055
NlaIV GGNNCC 4 cut(s) 104, 358, 1852, 2370
NmuCI GTSAC 2 cut(s) 1249, 1709
NsiI ATGCAT 1 cut(s) 1422
NspI RCATGY 3 cut(s) 1047, 2773, 3532
NspV TTCGAA 1 cut(s) 545
PaeR7I CTCGAG 1 cut(s) 2854
PagI TCATGA 5 cut(s) 1276, 1752, 2731, 3051, 3469
PciI ACATGT 1 cut(s) 3528
PciSI GCTCTTC 2 cut(s) 824, 2071
PctI GAATGC 1 cut(s) 178
PdmI GAANNNNTTC 4 cut(s) 2224, 2590, 2666, 3108
PflMI CCANNNNNTGG 2 cut(s) 874, 1459
PinAI ACCGGT 1 cut(s) 1757
PpuMI RGGWCCY 1 cut(s) 357
PscI ACATGT 1 cut(s) 3528
PshAI GACNNNNGTC 1 cut(s) 1256
Psp124BI GAGCTC 2 cut(s) 1386, 3099
Psp5II RGGWCCY 1 cut(s) 357
Psp6I CCWGG 7 cut(s) 710, 945, 1426, 1732, 2095, 2240, 2776
PspEI GGTNACC 1 cut(s) 3397
PspFI CCCAGC 1 cut(s) 1062
PspGI CCWGG 7 cut(s) 710, 945, 1426, 1732, 2095, 2240, 2776
PspN4I GGNNCC 4 cut(s) 104, 358, 1852, 2370
PspPI GGNCC 4 cut(s) 357, 2272, 2508, 3193
PspPPI RGGWCCY 1 cut(s) 357
PspXI VCTCGAGB 1 cut(s) 2854
PsrI GAACNNNNNNTAC 2 cut(s) 2730, 2762
PstI CTGCAG 3 cut(s) 1111, 1594, 1618
PstNI CAGNNNCTG 2 cut(s) 1876, 2188
PsuI RGATCY 4 cut(s) 1321, 1850, 2419, 2488
PvuII CAGCTG 1 cut(s) 2057
RseI CAYNNNNRTG 3 cut(s) 1281, 1928, 3533
SacI GAGCTC 2 cut(s) 1386, 3099
SalI GTCGAC 1 cut(s) 1335
SapI GCTCTTC 2 cut(s) 824, 2071
Sau96I GGNCC 4 cut(s) 357, 2272, 2508, 3193
ScaI AGTACT 1 cut(s) 1917
ScrFI CCNGG 7 cut(s) 712, 947, 1428, 1734, 2097, 2242, 2778
SduI GDGCHC 7 cut(s) 107, 1386, 1411, 1453, 2311, 3099, 3274
SexAI ACCWGGT 2 cut(s) 945, 1732
SfcI CTRYAG 4 cut(s) 132, 1107, 1590, 1614
Sfr274I CTCGAG 1 cut(s) 2854
SfuI TTCGAA 1 cut(s) 545
SinI GGWCC 4 cut(s) 357, 2272, 2508, 3193
SlaI CTCGAG 1 cut(s) 2854
SmiMI CAYNNNNRTG 3 cut(s) 1281, 1928, 3533
SmlI CTYRAG 6 cut(s) 1472, 2639, 2681, 2819, 2854, 3371
SmoI CTYRAG 6 cut(s) 1472, 2639, 2681, 2819, 2854, 3371
SsiI CCGC 6 cut(s) 380, 808, 1153, 2659, 3288, 3388
SstI GAGCTC 2 cut(s) 1386, 3099
StyD4I CCNGG 7 cut(s) 710, 945, 1426, 1732, 2095, 2240, 2776
StyI CCWWGG 5 cut(s) 1854, 2209, 2646, 2894, 3570
TaaI ACNGT 5 cut(s) 743, 772, 2414, 2434, 2534
TaiI ACGT 4 cut(s) 307, 1117, 1186, 1285
TaqI TCGA 9 cut(s) 236, 386, 545, 678, 1023, 1336, 2554, 2855, 3099
TaqII GACCGA 1 cut(s) 2260
TatI WGTACW 5 cut(s) 1002, 1196, 1702, 1915, 3354
TauI GCSGC 1 cut(s) 1156
TscAI CASTG 1 cut(s) 2539
TseFI GTSAC 2 cut(s) 1249, 1709
Tsp45I GTSAC 2 cut(s) 1249, 1709
TspGWI ACGGA 1 cut(s) 3357
TspRI CASTG 1 cut(s) 2539
Van91I CCANNNNNTGG 2 cut(s) 874, 1459
Vha464I CTTAAG 2 cut(s) 2639, 3371
VneI GTGCAC 1 cut(s) 1407
VpaK11BI GGWCC 4 cut(s) 357, 2272, 2508, 3193
XagI CCTNNNNNAGG 2 cut(s) 1896, 2684
XapI RAATTY 1 cut(s) 2326
XbaI TCTAGA 1 cut(s) 2416
XceI RCATGY 3 cut(s) 1047, 2773, 3532
XhoI CTCGAG 1 cut(s) 2854
XmaJI CCTAGG 1 cut(s) 2209
XmiI GTMKAC 3 cut(s) 640, 1336, 2130
XmnI GAANNNNTTC 4 cut(s) 2224, 2590, 2666, 3108
ZrmI AGTACT 1 cut(s) 1917
Zsp2I ATGCAT 1 cut(s) 1422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.