RchiOBHm_Chr7g0179211

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0013882
Physical Location & Seq
Reverse (-)
1572154 .. 1580144
7991 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ15973

Sequence Viewer

Length: 3678 bp
ATGCCTTACTCCGCCTTCTTCCACCGCTCTATAATAATCCGCACCACCTCCACCGCCATGTCCCTCAAACCCCGCCACCACCACTTCTCTCTCCTCCGGCCGCCGTCGCTCCTCCTCCTCGGATGCTTCATGAACACCAACGGCCACTACCACACTCACCCCGTCACCGGCCTGCTCAAATGGAGTTCCTCCGGCTGCTGCCGTCACGCTGACGTCACTTGCCTGGCCTCCTCGTCCTCGTCGCGTGCGGCGGAGAGGACGGATTGGAAGCAGCGGCGGAGGAGCGTTGCGGCCGTGCCGGCGTGGTACCAGCAGCAGCAGAAGCAGGGGTATGGGAGGTTTGCGTACCAGGACGAGTCGGGTAGTGAAGATTCGGACCGGGAGTTCGGGTCGGGTCAGAGCTCTCGCCGGGAGATGACTGGTTCGACCATTGACAACATCGATGAGTGGAGATGGAAGTTAACCATGCTTGTTCGCAACAAAGATGAGCAAGAAGTGGTGTCGAGGGACAGGAAGGACAGGCGTGATTTTGATCATCTTTCAGAACTGGCAAGAGGGATGGGTTTATATAGCCATCAGTATTCAAAGGTTGTAGTCTTTAGTAAAGTCCCGCAGCCAAATTACAGACCTGATCTGGATGATAGGCGACCACAAAGAGAGGTTGTCTTACCTTTTGGACTGCATAAAGATGTTGATGCTCATCTCAAAGCTCATCTTTCTCAAAAGCCAATCAGCAGAGGAAATTTATCGCATACTTCAATGTCAAGGTCAAATAGTAATGGAAGTATAGCTAAAGACGGGGGACTCTACGAGCAGGAGGAGCCCTTGATACAGAATAGTGTTGCCATGGAGAGAATTCTTCAGCAGAAAAGCTTGCGATTGCTCAATAAGCAACAAGAATGGCAGGAATCCATCGAAGGTCAAAAGATGCTTGAACTTCGTAGAAGTCTGCCTGCATATAAAGAGAAAGATTCCTTGTTAAAAGCCATATCCGAAAATCAGGTCATAGTTGTCTCAGGTGAAACAGGTTGTGGTAAAACTACACAACTTCCTCAGTACATTTTAGAATCTGAGATTGAAGCTGGTCGTGGAGGTGTCTGTAGTATTATTTGTACTCAGCCTAGACGAATATCTGCCATGTCTGTTTCTGAAAGAGTTGCTGCAGAACGAGGGGAGAATCTGGGGGAATCAGTTGGTTACAAAGTTAGGCTGGAAGGAATGAAAGGGAGGGACACACGGCTTCTTTTTTGCACCACAGGCATACTATTGAGAAGATTACTTGTCGACAGAAAGTTGAGAGGTGTTACACATGTCATTGTTGATGAGATTCATGAACGTGGAATGAATGAAGATTTTCTTCTTATCGTTCTGAAAGAACTTCTCTCTCGCCGTCCTGAGTTGAGATTGATTTTGATGAGTGCAACCTTAAATGCTGAGCTTTTCTCCTCGTACTTTTATGGTGCTCCAATGATCCATATACCTGGTTTTACATACCCGGTCCGAGCACATTTTCTGGAGAATATTCTAGAAATGACTGGATATCGGTTAAATCAATATAATCAAATTGATGATTATGGTCAAGATAAGACGTGGAAAATGCAGAAACAAGCTCAAGCTTTCAAAAAGAGGAAGAGCCAAATTGCTTCTACCGTTGAGGATGCACTTGAAGCTGCTGACTTTAGGGAGTACAGTCCAAGGACTCAGGAGTCTCTGTCCTGTTGGAATCCGGACTCCATTGGTTTTAATCTCATTGAGCATGTGCTTTGCCACATAGTCAGGAAAGAAAGGCCTGGTGCTATTTTGGTTTTTATGACTGGTTGGGATGACATAAACTCCTTGAAAGATCAACTCCAATCTCATCCCCTTTTAGGAGATCCTAGTAGGGTCCTGCTGCTTGCCTGTCATGGTTCCATGCCTAGCTCTGAACAGAGGTTGATATTTGATAAACCAGAAGATGGAGTTAGGAAAATCGTTCTGGCTACAAACATGGCTGAGACCAGTATCACTATCAATGATGTAGTATTTGTGATTGATTGTGGAAAGGCGAAAGAAACATCATATGATGCACTCAATAATACTCCCTGTTTGCTTCCATCCTGGATATCAAAGGCTGCTTCCCGGCAAAGAAGAGGAAGAGCCGGTCGTGTTCAACCCGGCGAGTGTTACCATCTATATCCCCGATGTGTATATGATGCCTTTGCTGATTATCAATTGCCTGAACTTTTGAGGACGCCTCTGCAGTCTCTATGTTTACAAATCAAGAGTCTACAACTTGGAAGTATTTCTGAGTTCCTTTCTAAGGCACTGCAGTCACCGGAACCTTTGTCGGTTCAAAATGCTGTTGATTATCTGAAGATCATAGGGGCTTTAGATGACAATGAAGATCTCACAGTATTAGGTCGCCACCTGTCAATGCTTCCTGTTGAGCCAAAACTTGGGAAAATGCTCATATTAGGGGCTATCTTCAACTGTTTAGATCCAATAATGACTATTGTTGCAGGCCTTAGTATGAGAGATCCATTCATGATGCCATTTGACAAGAAGGATCTTGCGGAATCGGCGAAAGCACAATTTTCTGGCCGTGATAGCAGCGATCATCTGGCTCTTATCCGAGCATATGATGGTTGGAAAAATGCTGAAAGATCGCAAGCTGGTTATGAGTACTGCTGGAGAAATTTTCTTTCTGCACAAACTCTAAAAGCCATTGACTCTCTTCGGAAGCAGTTCTTCTTCTTGCTCAAGGATGCTGGTTTGGTTGATCACAACATTGAGAACTGCAATACATTGAGCTATGATGAACAGCTTATCCGAGCAATCATCTGTGCAGGATTATTTCCTGGAATATGTTCTGTTGTGAACAAAGAGAAGTCGATTTCGTTGAAAACAATGGAAGATGGACAAGTACTTCTATACTCGAATTCTGTCAATGCTTCGGTGCCTAAAATTCCATATCCATGGCTAGTTTTCAATGAAAAGGTGAAAGTGAATTCAGTATTCCTTCGGGATTCAACCGGCGTATCTGATTCGGTGCTCCTTTTGTTTGGAGGCAACATTTCCAGGGGTGGACTGGATGGGCACCTGAAGATGTTGGGAGGATACTTGGAGTTTTTCATGAATCCTGCATTAGCAAATACATATGTTAGCTTAAAGAGGGAACTTGAGGAACTGATTCATAATAAGCTTATAGATCCCAAATTGGATATGCAATCTCATATTAACCTCCTATCAGCCCTAAGATTACTGGTCTCTGAAGACAGATGTGATGGTAGATTTGTCTTTGGTCGCAAGATGCCAGTACCCTCAAAGAAGATAACGAAAGAGTTAGGTCCAGGCACCTTGAGGGTTGGCGATAATGGTAAAAGCGGGGGTAACAATTCCAAAAGTCAGCTGCAAACATTGCTTGTCAGAGCAGGACATGAAGCTCCCACCTACAAAACAAAACAACTGAAAAACAACCAGTTTCGGTCCACAGTGATATTCAATGGGTTGAATTTTGTAGGGCAGCCTTCCAATAGTAAGAAACAAGCAGAGAAGGAAGCAGCTGCTGAAGCTGTGCTCTGGTTAAAGGGCGATAACCATTCATCTTCCACAGATATTGACCATATGTCAATGCTTCTAAACAAGAGTAGAAAGAAAATCCATAAAACGACCTCATTTGATAGCGCTAAGTGGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001047 GO:0001067 GO:0001503 GO:0001817 GO:0001819 GO:0002151 GO:0002791 GO:0002793 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003697 GO:0003723 GO:0003724 GO:0003725 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006355 GO:0006357 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0008186 GO:0009605 GO:0009607 GO:0009615 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010501 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010638 GO:0014070 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0019899 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031330 GO:0032204 GO:0032206 GO:0032479 GO:0032481 GO:0032501 GO:0032647 GO:0032727 GO:0032879 GO:0032880 GO:0033043 GO:0033044 GO:0034641 GO:0042221 GO:0042623 GO:0042826 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043330 GO:0043331 GO:0043487 GO:0043489 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050707 GO:0050708 GO:0050714 GO:0050715 GO:0050789 GO:0050794 GO:0050896 GO:0051046 GO:0051047 GO:0051049 GO:0051050 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051222 GO:0051223 GO:0051239 GO:0051240 GO:0051252 GO:0051253 GO:0051254 GO:0051704 GO:0051707 GO:0051880 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070035 GO:0070201 GO:0071704 GO:0080090 GO:0090087 GO:0090304 GO:0090669 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1902369 GO:1902680 GO:1902739 GO:1902741 GO:1903506 GO:1903508 GO:1903530 GO:1903532 GO:1904951 GO:1990837 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind dsrm

Protein Analysis

1225

Amino Acids

138.14

Weight (kDa)

8.97

Isoelectric Point (pI)

51.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 326 - 479 2.3e-10 DEAD/DEAH box helicase
Helicase_C PF00271 587 - 716 9.1e-13 Helicase conserved C-terminal domain
WHD_HA2 PF04408 780 - 808 3.3e-08 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 809 - 869 1.8e-14 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 936 - 1018 9.9e-20 Oligonucleotide/oligosaccharide-binding (OB)-fold
RNA_hel_CTD PF26026 1026 - 1084 5.7e-18 C-terminal domain in RNA helicases
dsrm PF00035 1127 - 1188 1.1e-08 Double-stranded RNA binding motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013882)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G48650 AT1G48650
fragaria_vesca FvH4_5g16860
malus_domestica MD06G1222500.v1.1 MD14G1231600.v1.1
prunus_persica Prupe.5G227100_v2.0.a1
pyrus_communis pycom06g19810 pycom06g19820 pycom14g19280
rosa_chinensis RchiOBHm_Chr7g0179211
rosa_laevigata RLG00000005374
rosa_multiflora Rmu_sc0009798.1_g000005 Rmu_sc0021799.1_g000001
rosa_roxburghii Rroxscaffold_3G00274220
rosa_rugosa Rorug06G0422200
rosa_samantha Rh7BG021500
rosa_wichuraiana Rw7G001770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1705
AatII GACGTC 1 cut(s) 216
Acc65I GGTACC 1 cut(s) 306
AccB1I GGYRCC 4 cut(s) 306, 2935, 3075, 3332
AccB7I CCANNNNNTGG 2 cut(s) 1955, 2435
AccBSI CCGCTC 1 cut(s) 27
AccI GTMKAC 2 cut(s) 1284, 2266
AccII CGCG 1 cut(s) 244
AccIII TCCGGA 1 cut(s) 1726
AclWI GGATC 6 cut(s) 1465, 1868, 2471, 2510, 2553, 3182
AcoI YGGCCR 4 cut(s) 98, 142, 291, 2578
AcsI RAATTY 7 cut(s) 742, 855, 2674, 2917, 2943, 2986, 3490
AcuI CTGAAG 5 cut(s) 845, 2372, 3101, 3270, 3567
AcyI GRCGYC 2 cut(s) 213, 2231
AfaI GTAC 9 cut(s) 308, 347, 1058, 1114, 1451, 1688, 2663, 2904, 3297
AfeI AGCGCT 1 cut(s) 3664
AfiI CCNNNNNNNGG 5 cut(s) 167, 1868, 1955, 2299, 2435
AflIII ACRYGT 1 cut(s) 1309
AhdI GACNNNNNGTC 1 cut(s) 3604
AjiI CACGTC 1 cut(s) 1590
AjnI CCWGG 8 cut(s) 222, 348, 1480, 1789, 2096, 2836, 3056, 3328
AloI GAACNNNNNNTCC 4 cut(s) 368, 400, 2790, 2822
Alw21I GWGCWC 5 cut(s) 404, 1465, 1507, 3033, 3558
Alw26I GTCTC 5 cut(s) 1018, 1713, 1988, 2247, 3250
AlwI GGATC 6 cut(s) 1465, 1868, 2471, 2510, 2553, 3182
AlwNI CAGNNNCTG 1 cut(s) 3545
Aor13HI TCCGGA 1 cut(s) 1726
Aor51HI AGCGCT 1 cut(s) 3664
AoxI GGCC 8 cut(s) 98, 142, 169, 225, 291, 1787, 2500, 2578
ApoI RAATTY 7 cut(s) 742, 855, 2674, 2917, 2943, 2986, 3490
ArsI GACNNNNNNTTYG 6 cut(s) 368, 400, 987, 1019, 2248, 2280
Asp700I GAANNNNTTC 5 cut(s) 1353, 2281, 2723, 2845, 3168
Asp718I GGTACC 1 cut(s) 306
AspLEI GCGC 1 cut(s) 3665
AspS9I GGNCC 5 cut(s) 376, 1498, 1885, 3326, 3465
AsuC2I CCSGG 5 cut(s) 380, 410, 1496, 2119, 2154
AsuHPI GGTGA 5 cut(s) 149, 157, 1031, 2304, 2989
AvaII GGWCC 5 cut(s) 376, 1498, 1885, 3326, 3465
BaeGI GKGCMC 1 cut(s) 3078
BaeI ACNNNNGTAYC 2 cut(s) 3000, 3033
BanI GGYRCC 4 cut(s) 306, 2935, 3075, 3332
BanII GRGCYC 2 cut(s) 404, 825
BbsI GAAGAC 1 cut(s) 3258
Bbv12I GWGCWC 5 cut(s) 404, 1465, 1507, 3033, 3558
BceAI ACGGC 7 cut(s) 88, 157, 186, 278, 1253, 1374, 2565
BciT130I CCWGG 8 cut(s) 224, 350, 1482, 1791, 2098, 2838, 3058, 3330
BciVI GTATCC 1 cut(s) 3089
BclI TGATCA 2 cut(s) 532, 2758
BcnI CCSGG 5 cut(s) 380, 410, 1496, 2119, 2154
BcoDI GTCTC 5 cut(s) 1018, 1713, 1988, 2247, 3250
BfaI CTAG 5 cut(s) 1122, 1526, 1878, 1917, 2960
BfmI CTRYAG 4 cut(s) 1099, 1161, 2237, 2306
BfoI RGCGCY 1 cut(s) 3666
BfuI GTATCC 1 cut(s) 3089
BglI GCCNNNNNGGC 1 cut(s) 299
BglII AGATCT 1 cut(s) 2383
BlpI GCTNAGC 1 cut(s) 1434
BmcAI AGTACT 2 cut(s) 2663, 2904
Bme18I GGWCC 5 cut(s) 376, 1498, 1885, 3326, 3465
BmeRI GACNNNNNGTC 1 cut(s) 3604
BmgBI CACGTC 1 cut(s) 1590
BmgT120I GGNCC 5 cut(s) 376, 1498, 1885, 3326, 3465
BmiI GGNNCC 8 cut(s) 308, 822, 1886, 1909, 2319, 2937, 3077, 3334
BmsI GCATC 9 cut(s) 113, 685, 918, 1648, 2053, 2182, 2517, 2734, 3279
BpiI GAAGAC 1 cut(s) 3258
BplI GAGNNNNNCTC 4 cut(s) 1427, 1459, 2218, 2250
BpmI CTGGAG 2 cut(s) 1535, 2689
Bpu1102I GCTNAGC 1 cut(s) 1434
BpuEI CTTGAG 4 cut(s) 1596, 2723, 3179, 3358
BpuMI CCSGG 5 cut(s) 380, 410, 1496, 2119, 2154
Bsa29I ATCGAT 1 cut(s) 441
BsaBI GATNNNNATC 2 cut(s) 531, 699
BsaHI GRCGYC 2 cut(s) 213, 2231
BsaI GGTCTC 2 cut(s) 1988, 3250
BsaJI CCNNGG 5 cut(s) 118, 846, 1694, 2954, 3057
BsaWI WCCGGW 2 cut(s) 1726, 2314
BsaXI ACNNNNNCTCC 6 cut(s) 374, 404, 1697, 1727, 1817, 1847
Bsc4I CCNNNNNNNGG 5 cut(s) 167, 1868, 1955, 2299, 2435
Bse118I RCCGGY 4 cut(s) 167, 298, 2138, 3011
Bse1I ACTGG 9 cut(s) 424, 552, 1540, 1819, 1998, 3072, 3246, 3293, 3457
Bse3DI GCAATG 1 cut(s) 3395
Bse8I GATNNNNATC 2 cut(s) 531, 699
BseAI TCCGGA 1 cut(s) 1726
BseBI CCWGG 8 cut(s) 224, 350, 1482, 1791, 2098, 2838, 3058, 3330
BseCI ATCGAT 1 cut(s) 441
BseDI CCNNGG 5 cut(s) 118, 846, 1694, 2954, 3057
BseGI GGATG 9 cut(s) 128, 564, 643, 1663, 1828, 1858, 2093, 2749, 3076
BseJI GATNNNNATC 2 cut(s) 531, 699
BseLI CCNNNNNNNGG 5 cut(s) 167, 1868, 1955, 2299, 2435
BseMI GCAATG 1 cut(s) 3395
BseMII CTCAG 9 cut(s) 1029, 1062, 1067, 1130, 1386, 1425, 1715, 1983, 2277
BseNI ACTGG 9 cut(s) 424, 552, 1540, 1819, 1998, 3072, 3246, 3293, 3457
BseRI GAGGAG 8 cut(s) 83, 101, 104, 107, 220, 295, 833, 1435
BseSI GKGCMC 1 cut(s) 3078
BseX3I CGGCCG 2 cut(s) 98, 291
BsgI GTGCAG 2 cut(s) 2670, 2844
Bsh1236I CGCG 1 cut(s) 244
Bsh1285I CGRYCG 3 cut(s) 101, 294, 2143
BshFI GGCC 8 cut(s) 100, 144, 171, 227, 293, 1789, 2502, 2580
BshNI GGYRCC 4 cut(s) 306, 2935, 3075, 3332
BshVI ATCGAT 1 cut(s) 441
BsiEI CGRYCG 3 cut(s) 101, 294, 2143
BsiHKAI GWGCWC 5 cut(s) 404, 1465, 1507, 3033, 3558
BslFI GGGAC 5 cut(s) 46, 521, 593, 816, 1244
BslI CCNNNNNNNGG 5 cut(s) 167, 1868, 1955, 2299, 2435
BsmAI GTCTC 5 cut(s) 1018, 1713, 1988, 2247, 3250
BsmFI GGGAC 5 cut(s) 46, 521, 593, 816, 1244
BsnI GGCC 8 cut(s) 100, 144, 171, 227, 293, 1789, 2502, 2580
Bso31I GGTCTC 2 cut(s) 1988, 3250
Bsp1286I GDGCHC 7 cut(s) 404, 825, 1465, 1507, 3033, 3078, 3558
Bsp13I TCCGGA 1 cut(s) 1726
Bsp1720I GCTNAGC 1 cut(s) 1434
Bsp19I CCATGG 2 cut(s) 846, 2954
BspANI GGCC 8 cut(s) 100, 144, 171, 227, 293, 1789, 2502, 2580
BspCNI CTCAG 9 cut(s) 1028, 1063, 1066, 1129, 1387, 1426, 1714, 1984, 2278
BspDI ATCGAT 1 cut(s) 441
BspEI TCCGGA 1 cut(s) 1726
BspFNI CGCG 1 cut(s) 244
BspHI TCATGA 4 cut(s) 129, 1330, 2523, 3111
BspLI GGNNCC 8 cut(s) 308, 822, 1886, 1909, 2319, 2937, 3077, 3334
BspMAI CTGCAG 3 cut(s) 1165, 2241, 2310
BspPI GGATC 6 cut(s) 1465, 1868, 2471, 2510, 2553, 3182
BspQI GCTCTTC 2 cut(s) 1625, 2128
BspT107I GGYRCC 4 cut(s) 306, 2935, 3075, 3332
BspTNI GGTCTC 2 cut(s) 1988, 3250
BsrBI CCGCTC 1 cut(s) 27
BsrDI GCAATG 1 cut(s) 3395
BsrFI RCCGGY 4 cut(s) 167, 298, 2138, 3011
BsrI ACTGG 9 cut(s) 424, 552, 1540, 1819, 1998, 3072, 3246, 3293, 3457
BssAI RCCGGY 4 cut(s) 167, 298, 2138, 3011
BssECI CCNNGG 5 cut(s) 118, 846, 1694, 2954, 3057
BssNI GRCGYC 2 cut(s) 213, 2231
BssT1I CCWWGG 3 cut(s) 846, 1694, 2954
Bst2UI CCWGG 8 cut(s) 224, 350, 1482, 1791, 2098, 2838, 3058, 3330
Bst4CI ACNGT 5 cut(s) 1651, 1691, 2392, 2471, 3472
Bst6I CTCTTC 4 cut(s) 1625, 2122, 2128, 2718
BstACI GRCGYC 2 cut(s) 213, 2231
BstAPI GCANNNNNTGC 1 cut(s) 3397
BstC8I GCNNGC 8 cut(s) 173, 246, 300, 875, 954, 1896, 2500, 2649
BstDSI CCRYGG 2 cut(s) 846, 2954
BstENI CCTNNNNNAGG 1 cut(s) 2297
BstF5I GGATG 9 cut(s) 128, 564, 643, 1663, 1828, 1858, 2093, 2749, 3076
BstFNI CGCG 1 cut(s) 244
BstH2I RGCGCY 1 cut(s) 3666
BstHHI GCGC 1 cut(s) 3665
BstMAI GTCTC 5 cut(s) 1018, 1713, 1988, 2247, 3250
BstMCI CGRYCG 3 cut(s) 101, 294, 2143
BstNI CCWGG 8 cut(s) 224, 350, 1482, 1791, 2098, 2838, 3058, 3330
BstNSI RCATGY 2 cut(s) 1313, 1760
BstSFI CTRYAG 4 cut(s) 1099, 1161, 2237, 2306
BstSLI GKGCMC 1 cut(s) 3078
BstUI CGCG 1 cut(s) 244
BstV2I GAAGAC 1 cut(s) 3258
BstX2I RGATCY 6 cut(s) 1873, 2383, 2476, 2515, 2545, 3187
BstXI CCANNNNNNTGG 2 cut(s) 1481, 2955
BstYI RGATCY 6 cut(s) 1873, 2383, 2476, 2515, 2545, 3187
BstZI CGGCCG 2 cut(s) 98, 291
Bsu15I ATCGAT 1 cut(s) 441
BsuI GTATCC 1 cut(s) 3089
BsuRI GGCC 8 cut(s) 100, 144, 171, 227, 293, 1789, 2502, 2580
BsuTUI ATCGAT 1 cut(s) 441
BtgI CCRYGG 2 cut(s) 846, 2954
BtrI CACGTC 1 cut(s) 1590
BtsCI GGATG 9 cut(s) 128, 564, 643, 1663, 1828, 1858, 2093, 2749, 3076
BtsI GCAGTG 1 cut(s) 2303
BtsIMutI CAGTG 2 cut(s) 2303, 3477
Cac8I GCNNGC 8 cut(s) 173, 246, 300, 875, 954, 1896, 2500, 2649
CaiI CAGNNNCTG 1 cut(s) 3545
CciI TCATGA 4 cut(s) 129, 1330, 2523, 3111
CfoI GCGC 1 cut(s) 3665
Cfr10I RCCGGY 4 cut(s) 167, 298, 2138, 3011
Cfr13I GGNCC 5 cut(s) 376, 1498, 1885, 3326, 3465
ClaI ATCGAT 1 cut(s) 441
CpoI CGGWCCG 2 cut(s) 376, 1498
CseI GACGC 1 cut(s) 2239
CsiI ACCWGGT 1 cut(s) 1480
Csp6I GTAC 9 cut(s) 307, 346, 1057, 1113, 1450, 1687, 2662, 2903, 3296
CspCI CAANNNNNGTGG 2 cut(s) 3577, 3612
CspI CGGWCCG 2 cut(s) 376, 1498
CviQI GTAC 9 cut(s) 307, 346, 1057, 1113, 1450, 1687, 2662, 2903, 3296
DrdI GACNNNNNNGTC 1 cut(s) 1705
DriI GACNNNNNGTC 1 cut(s) 3604
DseDI GACNNNNNNGTC 1 cut(s) 1705
EaeI YGGCCR 4 cut(s) 98, 142, 291, 2578
EagI CGGCCG 2 cut(s) 98, 291
Eam1104I CTCTTC 4 cut(s) 1625, 2122, 2128, 2718
Eam1105I GACNNNNNGTC 1 cut(s) 3604
EarI CTCTTC 4 cut(s) 1625, 2122, 2128, 2718
EciI GGCGGA 2 cut(s) 266, 292
Ecl136II GAGCTC 1 cut(s) 402
EclXI CGGCCG 2 cut(s) 98, 291
Eco130I CCWWGG 3 cut(s) 846, 1694, 2954
Eco147I AGGCCT 2 cut(s) 1789, 2502
Eco24I GRGCYC 2 cut(s) 404, 825
Eco31I GGTCTC 2 cut(s) 1988, 3250
Eco32I GATATC 2 cut(s) 1541, 2103
Eco47I GGWCC 5 cut(s) 376, 1498, 1885, 3326, 3465
Eco47III AGCGCT 1 cut(s) 3664
Eco52I CGGCCG 2 cut(s) 98, 291
Eco53kI GAGCTC 1 cut(s) 402
Eco57I CTGAAG 5 cut(s) 845, 2372, 3101, 3270, 3567
EcoICRI GAGCTC 1 cut(s) 402
EcoNI CCTNNNNNAGG 1 cut(s) 2297
EcoO109I RGGNCCY 1 cut(s) 1885
EcoRI GAATTC 3 cut(s) 855, 2917, 2986
EcoRII CCWGG 8 cut(s) 222, 348, 1480, 1789, 2096, 2836, 3056, 3328
EcoRV GATATC 2 cut(s) 1541, 2103
EcoT14I CCWWGG 3 cut(s) 846, 1694, 2954
EcoT38I GRGCYC 2 cut(s) 404, 825
ErhI CCWWGG 3 cut(s) 846, 1694, 2954
FalI AAGNNNNNCTT 6 cut(s) 699, 731, 1341, 1373, 2711, 2743
FaqI GGGAC 5 cut(s) 46, 521, 593, 816, 1244
FauI CCCGC 3 cut(s) 80, 618, 3356
FauNDI CATATG 4 cut(s) 2059, 2617, 3136, 3603
FbaI TGATCA 2 cut(s) 532, 2758
FblI GTMKAC 2 cut(s) 1284, 2266
FokI GGATG 9 cut(s) 135, 571, 650, 1670, 1835, 1845, 2080, 2756, 3083
FriOI GRGCYC 2 cut(s) 404, 825
FspBI CTAG 5 cut(s) 1122, 1526, 1878, 1917, 2960
GlaI GCGC 1 cut(s) 3664
GsuI CTGGAG 2 cut(s) 1535, 2689
HaeII RGCGCY 1 cut(s) 3666
HaeIII GGCC 8 cut(s) 100, 144, 171, 227, 293, 1789, 2502, 2580
HgaI GACGC 1 cut(s) 2239
HhaI GCGC 1 cut(s) 3665
Hin1I GRCGYC 2 cut(s) 213, 2231
Hin6I GCGC 1 cut(s) 3663
HinP1I GCGC 1 cut(s) 3663
HincII GTYRAC 2 cut(s) 462, 1285
HindII GTYRAC 2 cut(s) 462, 1285
HindIII AAGCTT 3 cut(s) 871, 1614, 3179
HpaI GTTAAC 1 cut(s) 462
HphI GGTGA 5 cut(s) 149, 157, 1031, 2304, 2989
Hpy166II GTNNAC 7 cut(s) 462, 1285, 2252, 2267, 2857, 3065, 3468
Hpy8I GTNNAC 7 cut(s) 462, 1285, 2252, 2267, 2857, 3065, 3468
Hpy99I CGWCG 2 cut(s) 109, 244
HpyAV CCTTC 8 cut(s) 25, 508, 911, 1208, 2536, 3008, 3516, 3526
HpyCH4III ACNGT 5 cut(s) 1651, 1691, 2392, 2471, 3472
HpyCH4IV ACGT 3 cut(s) 213, 1336, 1589
HpySE526I ACGT 3 cut(s) 213, 1336, 1589
Hsp92I GRCGYC 2 cut(s) 213, 2231
HspAI GCGC 1 cut(s) 3663
Kpn2I TCCGGA 1 cut(s) 1726
KpnI GGTACC 1 cut(s) 310
KroI GCCGGC 1 cut(s) 298
KroNI GCCGGC 1 cut(s) 300
Ksp22I TGATCA 2 cut(s) 532, 2758
KspAI GTTAAC 1 cut(s) 462
LguI GCTCTTC 2 cut(s) 1625, 2128
LmnI GCTCC 6 cut(s) 114, 282, 820, 1468, 3036, 3427
LweI GCATC 9 cut(s) 113, 685, 918, 1648, 2053, 2182, 2517, 2734, 3279
MabI ACCWGGT 1 cut(s) 1480
MaeI CTAG 5 cut(s) 1122, 1526, 1878, 1917, 2960
MaeII ACGT 3 cut(s) 213, 1336, 1589
MaeIII GTNAC 8 cut(s) 163, 203, 214, 1196, 1303, 2162, 2310, 3368
MbiI CCGCTC 1 cut(s) 27
MfeI CAATTG 1 cut(s) 2210
MflI RGATCY 6 cut(s) 1873, 2383, 2476, 2515, 2545, 3187
MhlI GDGCHC 7 cut(s) 404, 825, 1465, 1507, 3033, 3078, 3558
MlyI GAGTC 7 cut(s) 365, 798, 1693, 1715, 1724, 2272, 2702
MmeI TCCRAC 2 cut(s) 1700, 2606
MroI TCCGGA 1 cut(s) 1726
MroNI GCCGGC 1 cut(s) 298
MroXI GAANNNNTTC 5 cut(s) 1353, 2281, 2723, 2845, 3168
MseI TTAA 8 cut(s) 461, 980, 1427, 1547, 1743, 3146, 3216, 3563
MslI CAYNNNNRTG 4 cut(s) 56, 687, 1335, 2953
MspA1I CMGCKG 3 cut(s) 274, 3388, 3542
MunI CAATTG 1 cut(s) 2210
MvaI CCWGG 8 cut(s) 224, 350, 1482, 1791, 2098, 2838, 3058, 3330
MvnI CGCG 1 cut(s) 244
NaeI GCCGGC 1 cut(s) 300
NciI CCSGG 5 cut(s) 380, 410, 1496, 2119, 2154
NcoI CCATGG 2 cut(s) 846, 2954
NdeI CATATG 4 cut(s) 2059, 2617, 3136, 3603
NgoMIV GCCGGC 1 cut(s) 298
NlaIV GGNNCC 8 cut(s) 308, 822, 1886, 1909, 2319, 2937, 3077, 3334
NmuCI GTSAC 4 cut(s) 163, 203, 214, 2310
NspI RCATGY 2 cut(s) 1313, 1760
PagI TCATGA 4 cut(s) 129, 1330, 2523, 3111
PceI AGGCCT 2 cut(s) 1789, 2502
PciI ACATGT 1 cut(s) 1309
PciSI GCTCTTC 2 cut(s) 1625, 2128
PdiI GCCGGC 1 cut(s) 300
PdmI GAANNNNTTC 5 cut(s) 1353, 2281, 2723, 2845, 3168
PflMI CCANNNNNTGG 2 cut(s) 1955, 2435
PfoI TCCNGGA 2 cut(s) 2096, 2836
PleI GAGTC 7 cut(s) 364, 798, 1693, 1714, 1724, 2271, 2702
PpsI GAGTC 7 cut(s) 364, 798, 1693, 1714, 1724, 2271, 2702
PpuMI RGGWCCY 1 cut(s) 1885
PscI ACATGT 1 cut(s) 1309
Psp124BI GAGCTC 1 cut(s) 404
Psp5II RGGWCCY 1 cut(s) 1885
Psp6I CCWGG 8 cut(s) 222, 348, 1480, 1789, 2096, 2836, 3056, 3328
PspGI CCWGG 8 cut(s) 222, 348, 1480, 1789, 2096, 2836, 3056, 3328
PspN4I GGNNCC 8 cut(s) 308, 822, 1886, 1909, 2319, 2937, 3077, 3334
PspPI GGNCC 5 cut(s) 376, 1498, 1885, 3326, 3465
PspPPI RGGWCCY 1 cut(s) 1885
PstI CTGCAG 3 cut(s) 1165, 2241, 2310
PstNI CAGNNNCTG 1 cut(s) 3545
PsuI RGATCY 6 cut(s) 1873, 2383, 2476, 2515, 2545, 3187
PvuII CAGCTG 2 cut(s) 3388, 3542
RsaI GTAC 9 cut(s) 308, 347, 1058, 1114, 1451, 1688, 2663, 2904, 3297
RsaNI GTAC 9 cut(s) 307, 346, 1057, 1113, 1450, 1687, 2662, 2903, 3296
RseI CAYNNNNRTG 4 cut(s) 56, 687, 1335, 2953
Rsr2I CGGWCCG 2 cut(s) 376, 1498
RsrII CGGWCCG 2 cut(s) 376, 1498
SacI GAGCTC 1 cut(s) 404
SalI GTCGAC 1 cut(s) 1283
SapI GCTCTTC 2 cut(s) 1625, 2128
SaqAI TTAA 8 cut(s) 461, 980, 1427, 1547, 1743, 3146, 3216, 3563
Sau96I GGNCC 5 cut(s) 376, 1498, 1885, 3326, 3465
ScaI AGTACT 2 cut(s) 2663, 2904
SchI GAGTC 7 cut(s) 365, 798, 1693, 1715, 1724, 2272, 2702
SduI GDGCHC 7 cut(s) 404, 825, 1465, 1507, 3033, 3078, 3558
SexAI ACCWGGT 1 cut(s) 1480
SfaNI GCATC 9 cut(s) 113, 685, 918, 1648, 2053, 2182, 2517, 2734, 3279
SfcI CTRYAG 4 cut(s) 1099, 1161, 2237, 2306
SinI GGWCC 5 cut(s) 376, 1498, 1885, 3326, 3465
SmiMI CAYNNNNRTG 4 cut(s) 56, 687, 1335, 2953
SmlI CTYRAG 4 cut(s) 1611, 2738, 3158, 3337
SmoI CTYRAG 4 cut(s) 1611, 2738, 3158, 3337
SseBI AGGCCT 2 cut(s) 1789, 2502
SspI AATATT 1 cut(s) 1522
SspMI CTAG 5 cut(s) 1122, 1526, 1878, 1917, 2960
SstI GAGCTC 1 cut(s) 404
StuI AGGCCT 2 cut(s) 1789, 2502
StyI CCWWGG 3 cut(s) 846, 1694, 2954
TaaI ACNGT 5 cut(s) 1651, 1691, 2392, 2471, 3472
TaiI ACGT 3 cut(s) 216, 1339, 1592
TaqI TCGA 7 cut(s) 425, 441, 503, 915, 1284, 2870, 2915
TaqII GACCGA 1 cut(s) 3453
TatI WGTACW 5 cut(s) 1056, 1112, 1686, 2661, 2902
TauI GCSGC 4 cut(s) 103, 251, 277, 293
Tru1I TTAA 8 cut(s) 461, 980, 1427, 1547, 1743, 3146, 3216, 3563
Tru9I TTAA 8 cut(s) 461, 980, 1427, 1547, 1743, 3146, 3216, 3563
TscAI CASTG 2 cut(s) 2310, 3477
TseFI GTSAC 4 cut(s) 163, 203, 214, 2310
Tsp45I GTSAC 4 cut(s) 163, 203, 214, 2310
TspGWI ACGGA 1 cut(s) 275
TspRI CASTG 2 cut(s) 2310, 3477
Van91I CCANNNNNTGG 2 cut(s) 1955, 2435
VpaK11BI GGWCC 5 cut(s) 376, 1498, 1885, 3326, 3465
XagI CCTNNNNNAGG 1 cut(s) 2297
XapI RAATTY 7 cut(s) 742, 855, 2674, 2917, 2943, 2986, 3490
XbaI TCTAGA 1 cut(s) 1525
XceI RCATGY 2 cut(s) 1313, 1760
XcmI CCANNNNNNNNNTGG 1 cut(s) 3064
XmiI GTMKAC 2 cut(s) 1284, 2266
XmnI GAANNNNTTC 5 cut(s) 1353, 2281, 2723, 2845, 3168
XspI CTAG 5 cut(s) 1122, 1526, 1878, 1917, 2960
ZraI GACGTC 1 cut(s) 214
ZrmI AGTACT 2 cut(s) 2663, 2904
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.