Rroxscaffold_3G00274220

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0013882
Physical Location & Seq
Forward (+)
65867858 .. 65875767
7910 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00274220.1

Sequence Viewer

Length: 3684 bp
ATGCCTTACTCCGCCTTCTTTCACCGCTCTATAATTATCCGCACCACCTCCACCGCCATGTCCCTCAAACCCCGCCACCACCACTTCTCCCTCCTCCGGCCGCCCTCGCTCCTCCTCGGATGCTTCATGAACGCCAACGGCCACTACCACACTCACCCCGTCACCGGCTTGCTCAAATGGAATTCCGCCGCCGGCTGCTGCCGTCACGCTGACGTCACTTGCCTGGCCTCCTCGTCCTCCTCCTCGTCGCGTGCGGCGGAGAGGACGGATTGGAAGCAGCGGCGGAGGAGCGTTGCCGCCGTGCCGGCGTGGTACCAGCAGCAGCAGAAGCAGGGGTATGGGAGGTTTGCGTACCAGGACGAGTCGGGTAGTGAAGATTCGGACCGGGAGTTCGGGTCGGGTCAGAGCTCTCGCCGGGAAATGACTGGTTCGACCCTTGACAACATCGATGAGTGGAGATGGAAGTTAACCATGCTTGTTCGCAACAAAGATGAGCAAGAAGTGGTGTCGAGGGAAAGGAAGGACAGGCGTGATTTTGATCATCTTTCAGAACTGGCAAGAGGGATGGGTTTATATAGCCGTCAGTATTCAAAGGTTGTAGTCTTTAGTAAAGTCCCGCAGCCAAATTACAGACCTGATCTGGATGATAGGCGACCACAAAGAGAGGTTCTCTTACCTTTTGGACTGCATAAAGATGTAGATGCTCATCTCAAAGCTCATCTTTCTCAAAAGCCAATGAGCAGAGGAAATTTATCGCATACTTCAATGTCAAGGTCAAATAGTAATGGAAGTATAGCTAAAGACGGGGGACTCTACGAGCAGGAGGAGCCCTTGATACAGAATAGTGTTGCCATGGAGAGAATTCTTCAGCAGAAAAGCTTGCGATTGCTCAATAAGCAACAAGAATGGCAGGAATCCATCGAAGGTCAAAAGATGCTTGAACTTCGTAGAAGTCTGCCTGCATATAAAGAGAAAGATTCCTTGTTAAAAGCCATATCCGAAAATCAGGTCATAGTTGTCTCAGGTGAAACAGGTTGTGGTAAAACTACACAACTTCCTCAGTACATTTTAGAATCTGAGATTGAAGCTGGTCGTGGAGGTGTCTGTAGTATTATTTGTACTCAGCCTAGACGAATATCTGCCATGTCTGTTTCTGAAAGAGTTGCTGCAGAACGAGGGGAGAATCTGGGGGAATCAGTTGGTTACAAAGTTAGGCTGGAAGGAATGAAAGGGAGGGACACGCGGCTTCTTTTTTGCACCACAGGCATACTATTGAGAAGATTACTTGTCGACAGAAAGTTGAGAGGTGTTACACATGTCATTGTTGATGAGATTCATGAACGTGGAATGAATGAAGATTTTCTTCTTATCGTTCTGAAAGAACTTCTCTCTCGCCGTCCTGAGTTGAGATTGATTTTGATGAGTGCAACCTTAAATGCTGAGCTTTTCTCCTCGTACTTTAATGGTGCTCCAATGATCCATATACCTGGTTTTACATACCCGGTCCGAGCACATTTTCTGGAGAATATTCTAGAAATGACTGGATATCGGTTAAATCAATATAATCAAATTGATGATTATGGTCAAGATAAGACGTGGAAAATGCAGAAACAAGCTCAAGCTTTCAAAAAGAGGAAGAGCCAAATTGCTTCTACCGTTGAGGATGCACTTGAAGCTGCTGACTTTAGGGAGTACAGTCCACGGACTCAGGAGTCTCTGTCCTGTTGGAATCCGGACTCCATTGGTTTTAATCTCATTGAGCATGTGCTTTGCCACATAGTCAGGAAAGAAAGGCCTGGTGCTATTTTGGTTTTTATGACTGGCTGGGATGACATAAACTCCTTGAAGGATCAACTCCAATCTCATCCCCTTTTAGGAGATCCTAGTAGGGTCCTGCTGCTTGCCTGTCATGGTTCCATGCCTAGCTCTGAACAGAGGTTGATATTTGATAAACCAGAAGATGGAGTTAGGAAAATCGTTCTGGCTACAAACATGGCTGAGACTAGTATCACTATCAATGATGTAGTATTTGTGATTGATTGTGGAAAGGCGAAAGAAACATCATATGATGCACTCAATAATACTCCCTGTTTGCTTCCATCCTGGATATCAAAGGCTGCTTCCCGGCAAAGAAGAGGAAGAGCCGGTCGTGTTCAACCCGGCGAGTGTTACCATCTATATCCCCGATGTGTATATGATGCCTTTGCTGATTATCAATTGCCTGAACTTTTGAGGACGCCTCTGCAGTCTCTATGTTTACAAATCAAGAGTCTACAACTTGGAAGTATTTCTGAGTTCCTTTCTAAGGCACTGCAGTCACCGGAACCTTTGTCGGTTCAAAATGCTGTTGATTATCTAAAGATCATAGGGGCTTTAGATGACAATGAAGATCTCACAGTATTAGGTCGCCACCTGTCAATGCTTCCTGTTGAGCCAAAACTTGGGAAAATGCTCATATTAGGGGCTATCTTCAACTGTTTAGATCCAATAATGACTATTGTTGCAGGCCTTAGTATGAGAGATCCATTCATGATGCCATTTGACAAGAAGGATCTTGCGGAATCGGCAAAAGCACAATTTTCTGGCCATGATAGCAGCGATCATCTGGCTCTTATCCGAGCATATGATGGTTGGAAAAATGCTGAAAGATCGCAAGCTGGTTATGAGTACTGCTGGAGAAATTTTCTTTCTGCACAAACTCTAAAAGCCATTGACTCTCTTCGGAAGCAGTTCTTCTTCTTGCTCAAGGATGCTGGTTTGGTTGATCACAACATCGAGAACTGCAATACATTGAGCTATGATGAACAGCTTATCCGAGCAATCATCTGTGCAGGATTATTTCCTGGAATATGTTCTGTTGTGAACAAAGAGAAGTCGATTTCATTGAAAACGATGGAAGATGGACAAGTACTTCTATACTCGAATTCTGTCAATGCTTCGGTGCCTAAAATTCCATATCCATGGCTAGTTTTCAATGAAAAGGTGAAAGTGAATTCAGTATTCCTTCGGGATTCAACTGGCGTATCTGATTCTGTGCTCCTTTTGTTTGGAGGCAACATTTCCAGGGGTGGACTGGATGGGCACCTGAAGATGTTGGGAGGATACTTGGAGTTTTTCATGAATCCTGCATTAGCAAATACATATGTTAGCTTAAAGAGGGAACTTGAGGAACTGATTCATAATAAGCTTATAGATCCCAAATTGGATATGCAATCTCATACTAACCTCCTATCAGCCCTAAGATTACTGGTCTCTGAAGACAGATGTGATGGTAGATTTGTCTTTGGTCGCAAGATGCCAGTACCCGCAAAGAAGATAACCAAAGAGTTAGGTCCAGGCACCTTGAGGGTTGGCGATAATGGTAAAAGCGGGGGTAACAATTCCAAAAGTCAGCTGCAAACGTTGCTTGTCAGAGCAGGACATGAAGCTCCCACCTACAAAACAAAACAACTGAAAAACAACCAGTTTCGGTCCACAGTGATCTTCAATGGGTTGAACTTTGTAGGGCAGCCTTCCAATAGTAAGAAACAAGCAGAGAAGGAAGCGGCTGCTGAAGCTGTGCTCTGGTTAAAGGGCGATAACCATTCATCTTCCACAGATATTGACCATATGTCAATGCTTCTAAAGAAGAGTAGAAAGAAAATCCATAAAACGACCTCATTTGATAGCGCTAAGTGGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001047 GO:0001067 GO:0001503 GO:0001817 GO:0001819 GO:0002151 GO:0002791 GO:0002793 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003697 GO:0003723 GO:0003724 GO:0003725 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006355 GO:0006357 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0008186 GO:0009605 GO:0009607 GO:0009615 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010501 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010638 GO:0014070 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0019899 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031330 GO:0032204 GO:0032206 GO:0032479 GO:0032481 GO:0032501 GO:0032647 GO:0032727 GO:0032879 GO:0032880 GO:0033043 GO:0033044 GO:0034641 GO:0042221 GO:0042623 GO:0042826 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043330 GO:0043331 GO:0043487 GO:0043489 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050707 GO:0050708 GO:0050714 GO:0050715 GO:0050789 GO:0050794 GO:0050896 GO:0051046 GO:0051047 GO:0051049 GO:0051050 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051222 GO:0051223 GO:0051239 GO:0051240 GO:0051252 GO:0051253 GO:0051254 GO:0051704 GO:0051707 GO:0051880 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070035 GO:0070201 GO:0071704 GO:0080090 GO:0090087 GO:0090304 GO:0090669 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1902369 GO:1902680 GO:1902739 GO:1902741 GO:1903506 GO:1903508 GO:1903530 GO:1903532 GO:1904951 GO:1990837 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind dsrm

Protein Analysis

1227

Amino Acids

138.24

Weight (kDa)

9.0

Isoelectric Point (pI)

51.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 328 - 481 2.4e-10 DEAD/DEAH box helicase
Helicase_C PF00271 589 - 718 9.1e-13 Helicase conserved C-terminal domain
WHD_HA2 PF04408 782 - 810 3.3e-08 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 811 - 871 1.7e-14 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 938 - 1020 9.9e-20 Oligonucleotide/oligosaccharide-binding (OB)-fold
RNA_hel_CTD PF26026 1028 - 1086 2.1e-18 C-terminal domain in RNA helicases
dsrm PF00035 1129 - 1190 1.1e-08 Double-stranded RNA binding motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013882)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G48650 AT1G48650
fragaria_vesca FvH4_5g16860
malus_domestica MD06G1222500.v1.1 MD14G1231600.v1.1
prunus_persica Prupe.5G227100_v2.0.a1
pyrus_communis pycom06g19810 pycom06g19820 pycom14g19280
rosa_chinensis RchiOBHm_Chr7g0179211
rosa_laevigata RLG00000005374
rosa_multiflora Rmu_sc0009798.1_g000005 Rmu_sc0021799.1_g000001
rosa_roxburghii Rroxscaffold_3G00274220
rosa_rugosa Rorug06G0422200
rosa_samantha Rh7BG021500
rosa_wichuraiana Rw7G001770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1711
AatII GACGTC 1 cut(s) 216
Acc65I GGTACC 1 cut(s) 312
AccB1I GGYRCC 4 cut(s) 312, 2941, 3081, 3338
AccB7I CCANNNNNTGG 2 cut(s) 1961, 2441
AccBSI CCGCTC 1 cut(s) 27
AccI GTMKAC 2 cut(s) 1290, 2272
AccII CGCG 2 cut(s) 250, 1243
AccIII TCCGGA 1 cut(s) 1732
AclI AACGTT 1 cut(s) 3401
AclWI GGATC 7 cut(s) 1471, 1857, 1874, 2477, 2516, 2559, 3188
AcoI YGGCCR 3 cut(s) 98, 139, 2584
AcsI RAATTY 7 cut(s) 181, 748, 861, 2680, 2923, 2949, 2992
AcuI CTGAAG 4 cut(s) 851, 3107, 3276, 3573
AcyI GRCGYC 2 cut(s) 213, 2237
AfaI GTAC 9 cut(s) 314, 353, 1064, 1120, 1457, 1694, 2669, 2910, 3303
AfeI AGCGCT 1 cut(s) 3670
AfiI CCNNNNNNNGG 6 cut(s) 96, 164, 1874, 1961, 2305, 2441
AflIII ACRYGT 1 cut(s) 1315
AhdI GACNNNNNGTC 1 cut(s) 3610
AhlI ACTAGT 1 cut(s) 2003
AjiI CACGTC 1 cut(s) 1596
AjnI CCWGG 8 cut(s) 222, 354, 1486, 1795, 2102, 2842, 3062, 3334
AloI GAACNNNNNNTCC 4 cut(s) 374, 406, 2796, 2828
Alw21I GWGCWC 5 cut(s) 410, 1471, 1513, 3039, 3564
Alw26I GTCTC 5 cut(s) 1024, 1719, 1994, 2253, 3256
AlwI GGATC 7 cut(s) 1471, 1857, 1874, 2477, 2516, 2559, 3188
Aor13HI TCCGGA 1 cut(s) 1732
Aor51HI AGCGCT 1 cut(s) 3670
AoxI GGCC 6 cut(s) 98, 139, 225, 1793, 2506, 2584
ApoI RAATTY 7 cut(s) 181, 748, 861, 2680, 2923, 2949, 2992
ArsI GACNNNNNNTTYG 6 cut(s) 374, 406, 993, 1025, 2254, 2286
Asp700I GAANNNNTTC 5 cut(s) 1359, 2287, 2729, 2851, 3174
Asp718I GGTACC 1 cut(s) 312
AspLEI GCGC 1 cut(s) 3671
AspS9I GGNCC 5 cut(s) 382, 1504, 1891, 3332, 3471
AsuC2I CCSGG 5 cut(s) 386, 416, 1502, 2125, 2160
AsuHPI GGTGA 6 cut(s) 14, 146, 154, 1037, 2310, 2995
AvaII GGWCC 5 cut(s) 382, 1504, 1891, 3332, 3471
BaeGI GKGCMC 1 cut(s) 3084
BaeI ACNNNNGTAYC 2 cut(s) 3006, 3039
BalI TGGCCA 1 cut(s) 2586
BanI GGYRCC 4 cut(s) 312, 2941, 3081, 3338
BanII GRGCYC 2 cut(s) 410, 831
BbsI GAAGAC 1 cut(s) 3264
Bbv12I GWGCWC 5 cut(s) 410, 1471, 1513, 3039, 3564
BceAI ACGGC 5 cut(s) 154, 186, 284, 564, 1380
BciT130I CCWGG 8 cut(s) 224, 356, 1488, 1797, 2104, 2844, 3064, 3336
BciVI GTATCC 1 cut(s) 3095
BclI TGATCA 2 cut(s) 538, 2764
BcnI CCSGG 5 cut(s) 386, 416, 1502, 2125, 2160
BcoDI GTCTC 5 cut(s) 1024, 1719, 1994, 2253, 3256
BcuI ACTAGT 1 cut(s) 2003
BfaI CTAG 6 cut(s) 1128, 1532, 1884, 1923, 2004, 2966
BfmI CTRYAG 4 cut(s) 1105, 1167, 2243, 2312
BfoI RGCGCY 1 cut(s) 3672
BfuI GTATCC 1 cut(s) 3095
BglI GCCNNNNNGGC 1 cut(s) 305
BglII AGATCT 1 cut(s) 2389
BlpI GCTNAGC 1 cut(s) 1440
BmcAI AGTACT 2 cut(s) 2669, 2910
Bme18I GGWCC 5 cut(s) 382, 1504, 1891, 3332, 3471
BmeRI GACNNNNNGTC 1 cut(s) 3610
BmgBI CACGTC 1 cut(s) 1596
BmgT120I GGNCC 5 cut(s) 382, 1504, 1891, 3332, 3471
BmiI GGNNCC 8 cut(s) 314, 828, 1892, 1915, 2325, 2943, 3083, 3340
BmsI GCATC 9 cut(s) 110, 691, 924, 1654, 2059, 2188, 2523, 2740, 3285
BpiI GAAGAC 1 cut(s) 3264
BplI GAGNNNNNCTC 6 cut(s) 654, 686, 1433, 1465, 2224, 2256
BpmI CTGGAG 2 cut(s) 1541, 2695
Bpu1102I GCTNAGC 1 cut(s) 1440
BpuEI CTTGAG 4 cut(s) 1602, 2729, 3185, 3364
BpuMI CCSGG 5 cut(s) 386, 416, 1502, 2125, 2160
Bsa29I ATCGAT 1 cut(s) 447
BsaBI GATNNNNATC 2 cut(s) 537, 705
BsaHI GRCGYC 2 cut(s) 213, 2237
BsaI GGTCTC 1 cut(s) 3256
BsaJI CCNNGG 5 cut(s) 115, 852, 1700, 2960, 3063
BsaWI WCCGGW 2 cut(s) 1732, 2320
BsaXI ACNNNNNCTCC 8 cut(s) 71, 101, 380, 410, 1703, 1733, 1823, 1853
Bsc4I CCNNNNNNNGG 6 cut(s) 96, 164, 1874, 1961, 2305, 2441
Bse118I RCCGGY 4 cut(s) 164, 191, 304, 2144
Bse1I ACTGG 9 cut(s) 430, 558, 1546, 1825, 3022, 3078, 3252, 3299, 3463
Bse8I GATNNNNATC 2 cut(s) 537, 705
BseAI TCCGGA 1 cut(s) 1732
BseBI CCWGG 8 cut(s) 224, 356, 1488, 1797, 2104, 2844, 3064, 3336
BseCI ATCGAT 1 cut(s) 447
BseDI CCNNGG 5 cut(s) 115, 852, 1700, 2960, 3063
BseGI GGATG 9 cut(s) 125, 570, 649, 1669, 1834, 1864, 2099, 2755, 3082
BseJI GATNNNNATC 2 cut(s) 537, 705
BseLI CCNNNNNNNGG 6 cut(s) 96, 164, 1874, 1961, 2305, 2441
BseMII CTCAG 9 cut(s) 1035, 1068, 1073, 1136, 1392, 1431, 1721, 1989, 2283
BseNI ACTGG 9 cut(s) 430, 558, 1546, 1825, 3022, 3078, 3252, 3299, 3463
BseRI GAGGAG 9 cut(s) 83, 101, 104, 220, 229, 232, 301, 839, 1441
BseSI GKGCMC 1 cut(s) 3084
BseX3I CGGCCG 1 cut(s) 98
BseYI CCCAGC 1 cut(s) 1824
BsgI GTGCAG 2 cut(s) 2676, 2850
Bsh1236I CGCG 2 cut(s) 250, 1243
Bsh1285I CGRYCG 2 cut(s) 101, 2149
BshFI GGCC 6 cut(s) 100, 141, 227, 1795, 2508, 2586
BshNI GGYRCC 4 cut(s) 312, 2941, 3081, 3338
BshVI ATCGAT 1 cut(s) 447
BsiEI CGRYCG 2 cut(s) 101, 2149
BsiHKAI GWGCWC 5 cut(s) 410, 1471, 1513, 3039, 3564
BslFI GGGAC 4 cut(s) 46, 599, 822, 1250
BslI CCNNNNNNNGG 6 cut(s) 96, 164, 1874, 1961, 2305, 2441
BsmAI GTCTC 5 cut(s) 1024, 1719, 1994, 2253, 3256
BsmFI GGGAC 4 cut(s) 46, 599, 822, 1250
BsnI GGCC 6 cut(s) 100, 141, 227, 1795, 2508, 2586
Bso31I GGTCTC 1 cut(s) 3256
Bsp1286I GDGCHC 7 cut(s) 410, 831, 1471, 1513, 3039, 3084, 3564
Bsp13I TCCGGA 1 cut(s) 1732
Bsp1720I GCTNAGC 1 cut(s) 1440
Bsp19I CCATGG 2 cut(s) 852, 2960
BspANI GGCC 6 cut(s) 100, 141, 227, 1795, 2508, 2586
BspCNI CTCAG 9 cut(s) 1034, 1069, 1072, 1135, 1393, 1432, 1720, 1990, 2284
BspDI ATCGAT 1 cut(s) 447
BspEI TCCGGA 1 cut(s) 1732
BspFNI CGCG 2 cut(s) 250, 1243
BspHI TCATGA 4 cut(s) 126, 1336, 2529, 3117
BspLI GGNNCC 8 cut(s) 314, 828, 1892, 1915, 2325, 2943, 3083, 3340
BspMAI CTGCAG 3 cut(s) 1171, 2247, 2316
BspPI GGATC 7 cut(s) 1471, 1857, 1874, 2477, 2516, 2559, 3188
BspQI GCTCTTC 2 cut(s) 1631, 2134
BspT107I GGYRCC 4 cut(s) 312, 2941, 3081, 3338
BspTNI GGTCTC 1 cut(s) 3256
BsrBI CCGCTC 1 cut(s) 27
BsrFI RCCGGY 4 cut(s) 164, 191, 304, 2144
BsrI ACTGG 9 cut(s) 430, 558, 1546, 1825, 3022, 3078, 3252, 3299, 3463
BssAI RCCGGY 4 cut(s) 164, 191, 304, 2144
BssECI CCNNGG 5 cut(s) 115, 852, 1700, 2960, 3063
BssNI GRCGYC 2 cut(s) 213, 2237
BssT1I CCWWGG 2 cut(s) 852, 2960
Bst2UI CCWGG 8 cut(s) 224, 356, 1488, 1797, 2104, 2844, 3064, 3336
Bst4CI ACNGT 5 cut(s) 1657, 1697, 2398, 2477, 3478
Bst6I CTCTTC 5 cut(s) 1631, 2128, 2134, 2724, 3623
BstACI GRCGYC 2 cut(s) 213, 2237
BstAPI GCANNNNNTGC 1 cut(s) 3403
BstC8I GCNNGC 9 cut(s) 170, 193, 252, 306, 881, 960, 1902, 2506, 2655
BstDSI CCRYGG 3 cut(s) 852, 1700, 2960
BstENI CCTNNNNNAGG 1 cut(s) 2303
BstF5I GGATG 9 cut(s) 125, 570, 649, 1669, 1834, 1864, 2099, 2755, 3082
BstFNI CGCG 2 cut(s) 250, 1243
BstH2I RGCGCY 1 cut(s) 3672
BstHHI GCGC 1 cut(s) 3671
BstMAI GTCTC 5 cut(s) 1024, 1719, 1994, 2253, 3256
BstMCI CGRYCG 2 cut(s) 101, 2149
BstNI CCWGG 8 cut(s) 224, 356, 1488, 1797, 2104, 2844, 3064, 3336
BstNSI RCATGY 2 cut(s) 1319, 1766
BstSFI CTRYAG 4 cut(s) 1105, 1167, 2243, 2312
BstSLI GKGCMC 1 cut(s) 3084
BstUI CGCG 2 cut(s) 250, 1243
BstV2I GAAGAC 1 cut(s) 3264
BstX2I RGATCY 6 cut(s) 1879, 2389, 2482, 2521, 2551, 3193
BstXI CCANNNNNNTGG 2 cut(s) 1487, 2961
BstYI RGATCY 6 cut(s) 1879, 2389, 2482, 2521, 2551, 3193
BstZI CGGCCG 1 cut(s) 98
Bsu15I ATCGAT 1 cut(s) 447
BsuI GTATCC 1 cut(s) 3095
BsuRI GGCC 6 cut(s) 100, 141, 227, 1795, 2508, 2586
BsuTUI ATCGAT 1 cut(s) 447
BtgI CCRYGG 3 cut(s) 852, 1700, 2960
BtrI CACGTC 1 cut(s) 1596
BtsCI GGATG 9 cut(s) 125, 570, 649, 1669, 1834, 1864, 2099, 2755, 3082
BtsI GCAGTG 1 cut(s) 2309
BtsIMutI CAGTG 2 cut(s) 2309, 3483
Cac8I GCNNGC 9 cut(s) 170, 193, 252, 306, 881, 960, 1902, 2506, 2655
CciI TCATGA 4 cut(s) 126, 1336, 2529, 3117
CfoI GCGC 1 cut(s) 3671
Cfr10I RCCGGY 4 cut(s) 164, 191, 304, 2144
Cfr13I GGNCC 5 cut(s) 382, 1504, 1891, 3332, 3471
ClaI ATCGAT 1 cut(s) 447
CpoI CGGWCCG 2 cut(s) 382, 1504
CseI GACGC 1 cut(s) 2245
CsiI ACCWGGT 1 cut(s) 1486
Csp6I GTAC 9 cut(s) 313, 352, 1063, 1119, 1456, 1693, 2668, 2909, 3302
CspCI CAANNNNNGTGG 2 cut(s) 3583, 3618
CspI CGGWCCG 2 cut(s) 382, 1504
CviQI GTAC 9 cut(s) 313, 352, 1063, 1119, 1456, 1693, 2668, 2909, 3302
DrdI GACNNNNNNGTC 1 cut(s) 1711
DriI GACNNNNNGTC 1 cut(s) 3610
DseDI GACNNNNNNGTC 1 cut(s) 1711
EaeI YGGCCR 3 cut(s) 98, 139, 2584
EagI CGGCCG 1 cut(s) 98
Eam1104I CTCTTC 5 cut(s) 1631, 2128, 2134, 2724, 3623
Eam1105I GACNNNNNGTC 1 cut(s) 3610
EarI CTCTTC 5 cut(s) 1631, 2128, 2134, 2724, 3623
EciI GGCGGA 3 cut(s) 175, 272, 298
Ecl136II GAGCTC 1 cut(s) 408
EclXI CGGCCG 1 cut(s) 98
Eco130I CCWWGG 2 cut(s) 852, 2960
Eco147I AGGCCT 2 cut(s) 1795, 2508
Eco24I GRGCYC 2 cut(s) 410, 831
Eco31I GGTCTC 1 cut(s) 3256
Eco32I GATATC 2 cut(s) 1547, 2109
Eco47I GGWCC 5 cut(s) 382, 1504, 1891, 3332, 3471
Eco47III AGCGCT 1 cut(s) 3670
Eco52I CGGCCG 1 cut(s) 98
Eco53kI GAGCTC 1 cut(s) 408
Eco57I CTGAAG 4 cut(s) 851, 3107, 3276, 3573
EcoICRI GAGCTC 1 cut(s) 408
EcoNI CCTNNNNNAGG 1 cut(s) 2303
EcoO109I RGGNCCY 1 cut(s) 1891
EcoRI GAATTC 4 cut(s) 181, 861, 2923, 2992
EcoRII CCWGG 8 cut(s) 222, 354, 1486, 1795, 2102, 2842, 3062, 3334
EcoRV GATATC 2 cut(s) 1547, 2109
EcoT14I CCWWGG 2 cut(s) 852, 2960
EcoT38I GRGCYC 2 cut(s) 410, 831
ErhI CCWWGG 2 cut(s) 852, 2960
FalI AAGNNNNNCTT 6 cut(s) 705, 737, 1347, 1379, 2717, 2749
FaqI GGGAC 4 cut(s) 46, 599, 822, 1250
FauI CCCGC 4 cut(s) 80, 624, 3313, 3362
FauNDI CATATG 4 cut(s) 2065, 2623, 3142, 3609
FbaI TGATCA 2 cut(s) 538, 2764
FblI GTMKAC 2 cut(s) 1290, 2272
FokI GGATG 9 cut(s) 132, 577, 656, 1676, 1841, 1851, 2086, 2762, 3089
FriOI GRGCYC 2 cut(s) 410, 831
FspBI CTAG 6 cut(s) 1128, 1532, 1884, 1923, 2004, 2966
GlaI GCGC 1 cut(s) 3670
GsaI CCCAGC 1 cut(s) 1828
GsuI CTGGAG 2 cut(s) 1541, 2695
HaeII RGCGCY 1 cut(s) 3672
HaeIII GGCC 6 cut(s) 100, 141, 227, 1795, 2508, 2586
HgaI GACGC 1 cut(s) 2245
HhaI GCGC 1 cut(s) 3671
Hin1I GRCGYC 2 cut(s) 213, 2237
Hin6I GCGC 1 cut(s) 3669
HinP1I GCGC 1 cut(s) 3669
HincII GTYRAC 2 cut(s) 468, 1291
HindII GTYRAC 2 cut(s) 468, 1291
HindIII AAGCTT 3 cut(s) 877, 1620, 3185
HpaI GTTAAC 1 cut(s) 468
HphI GGTGA 6 cut(s) 14, 146, 154, 1037, 2310, 2995
Hpy166II GTNNAC 8 cut(s) 468, 1291, 1700, 2258, 2273, 2863, 3071, 3474
Hpy8I GTNNAC 8 cut(s) 468, 1291, 1700, 2258, 2273, 2863, 3071, 3474
Hpy99I CGWCG 1 cut(s) 250
HpyAV CCTTC 9 cut(s) 25, 514, 917, 1214, 1840, 2542, 3014, 3522, 3532
HpyCH4III ACNGT 5 cut(s) 1657, 1697, 2398, 2477, 3478
HpyCH4IV ACGT 4 cut(s) 213, 1342, 1595, 3401
HpySE526I ACGT 4 cut(s) 213, 1342, 1595, 3401
Hsp92I GRCGYC 2 cut(s) 213, 2237
HspAI GCGC 1 cut(s) 3669
Kpn2I TCCGGA 1 cut(s) 1732
KpnI GGTACC 1 cut(s) 316
KroI GCCGGC 2 cut(s) 191, 304
KroNI GCCGGC 2 cut(s) 193, 306
Ksp22I TGATCA 2 cut(s) 538, 2764
KspAI GTTAAC 1 cut(s) 468
LguI GCTCTTC 2 cut(s) 1631, 2134
LmnI GCTCC 6 cut(s) 114, 288, 826, 1474, 3042, 3433
LweI GCATC 9 cut(s) 110, 691, 924, 1654, 2059, 2188, 2523, 2740, 3285
MabI ACCWGGT 1 cut(s) 1486
MaeI CTAG 6 cut(s) 1128, 1532, 1884, 1923, 2004, 2966
MaeII ACGT 4 cut(s) 213, 1342, 1595, 3401
MaeIII GTNAC 8 cut(s) 160, 203, 214, 1202, 1309, 2168, 2316, 3374
MbiI CCGCTC 1 cut(s) 27
MfeI CAATTG 1 cut(s) 2216
MflI RGATCY 6 cut(s) 1879, 2389, 2482, 2521, 2551, 3193
MhlI GDGCHC 7 cut(s) 410, 831, 1471, 1513, 3039, 3084, 3564
MlsI TGGCCA 1 cut(s) 2586
MluNI TGGCCA 1 cut(s) 2586
MlyI GAGTC 7 cut(s) 371, 804, 1699, 1721, 1730, 2278, 2708
MmeI TCCRAC 2 cut(s) 1706, 2612
Mox20I TGGCCA 1 cut(s) 2586
MroI TCCGGA 1 cut(s) 1732
MroNI GCCGGC 2 cut(s) 191, 304
MroXI GAANNNNTTC 5 cut(s) 1359, 2287, 2729, 2851, 3174
MscI TGGCCA 1 cut(s) 2586
MseI TTAA 8 cut(s) 467, 986, 1433, 1461, 1553, 1749, 3152, 3569
MslI CAYNNNNRTG 4 cut(s) 56, 693, 1341, 2959
Msp20I TGGCCA 1 cut(s) 2586
MspA1I CMGCKG 2 cut(s) 280, 3394
MunI CAATTG 1 cut(s) 2216
MvaI CCWGG 8 cut(s) 224, 356, 1488, 1797, 2104, 2844, 3064, 3336
MvnI CGCG 2 cut(s) 250, 1243
NaeI GCCGGC 2 cut(s) 193, 306
NciI CCSGG 5 cut(s) 386, 416, 1502, 2125, 2160
NcoI CCATGG 2 cut(s) 852, 2960
NdeI CATATG 4 cut(s) 2065, 2623, 3142, 3609
NgoMIV GCCGGC 2 cut(s) 191, 304
NlaIV GGNNCC 8 cut(s) 314, 828, 1892, 1915, 2325, 2943, 3083, 3340
NmuCI GTSAC 4 cut(s) 160, 203, 214, 2316
NspI RCATGY 2 cut(s) 1319, 1766
PagI TCATGA 4 cut(s) 126, 1336, 2529, 3117
PceI AGGCCT 2 cut(s) 1795, 2508
PciI ACATGT 1 cut(s) 1315
PciSI GCTCTTC 2 cut(s) 1631, 2134
PdiI GCCGGC 2 cut(s) 193, 306
PdmI GAANNNNTTC 5 cut(s) 1359, 2287, 2729, 2851, 3174
PflMI CCANNNNNTGG 2 cut(s) 1961, 2441
PfoI TCCNGGA 2 cut(s) 2102, 2842
PleI GAGTC 7 cut(s) 370, 804, 1699, 1720, 1730, 2277, 2708
PpsI GAGTC 7 cut(s) 370, 804, 1699, 1720, 1730, 2277, 2708
PpuMI RGGWCCY 1 cut(s) 1891
PscI ACATGT 1 cut(s) 1315
Psp124BI GAGCTC 1 cut(s) 410
Psp1406I AACGTT 1 cut(s) 3401
Psp5II RGGWCCY 1 cut(s) 1891
Psp6I CCWGG 8 cut(s) 222, 354, 1486, 1795, 2102, 2842, 3062, 3334
PspFI CCCAGC 1 cut(s) 1824
PspGI CCWGG 8 cut(s) 222, 354, 1486, 1795, 2102, 2842, 3062, 3334
PspN4I GGNNCC 8 cut(s) 314, 828, 1892, 1915, 2325, 2943, 3083, 3340
PspPI GGNCC 5 cut(s) 382, 1504, 1891, 3332, 3471
PspPPI RGGWCCY 1 cut(s) 1891
PstI CTGCAG 3 cut(s) 1171, 2247, 2316
PsuI RGATCY 6 cut(s) 1879, 2389, 2482, 2521, 2551, 3193
PvuII CAGCTG 1 cut(s) 3394
RsaI GTAC 9 cut(s) 314, 353, 1064, 1120, 1457, 1694, 2669, 2910, 3303
RsaNI GTAC 9 cut(s) 313, 352, 1063, 1119, 1456, 1693, 2668, 2909, 3302
RseI CAYNNNNRTG 4 cut(s) 56, 693, 1341, 2959
Rsr2I CGGWCCG 2 cut(s) 382, 1504
RsrII CGGWCCG 2 cut(s) 382, 1504
SacI GAGCTC 1 cut(s) 410
SalI GTCGAC 1 cut(s) 1289
SapI GCTCTTC 2 cut(s) 1631, 2134
SaqAI TTAA 8 cut(s) 467, 986, 1433, 1461, 1553, 1749, 3152, 3569
Sau96I GGNCC 5 cut(s) 382, 1504, 1891, 3332, 3471
ScaI AGTACT 2 cut(s) 2669, 2910
SchI GAGTC 7 cut(s) 371, 804, 1699, 1721, 1730, 2278, 2708
SduI GDGCHC 7 cut(s) 410, 831, 1471, 1513, 3039, 3084, 3564
SexAI ACCWGGT 1 cut(s) 1486
SfaNI GCATC 9 cut(s) 110, 691, 924, 1654, 2059, 2188, 2523, 2740, 3285
SfcI CTRYAG 4 cut(s) 1105, 1167, 2243, 2312
SinI GGWCC 5 cut(s) 382, 1504, 1891, 3332, 3471
SmiMI CAYNNNNRTG 4 cut(s) 56, 693, 1341, 2959
SmlI CTYRAG 4 cut(s) 1617, 2744, 3164, 3343
SmoI CTYRAG 4 cut(s) 1617, 2744, 3164, 3343
SpeI ACTAGT 1 cut(s) 2003
SseBI AGGCCT 2 cut(s) 1795, 2508
SspI AATATT 1 cut(s) 1528
SspMI CTAG 6 cut(s) 1128, 1532, 1884, 1923, 2004, 2966
SstI GAGCTC 1 cut(s) 410
StuI AGGCCT 2 cut(s) 1795, 2508
StyI CCWWGG 2 cut(s) 852, 2960
TaaI ACNGT 5 cut(s) 1657, 1697, 2398, 2477, 3478
TaiI ACGT 4 cut(s) 216, 1345, 1598, 3404
TaqI TCGA 8 cut(s) 431, 447, 509, 921, 1290, 2775, 2876, 2921
TaqII GACCGA 1 cut(s) 3459
TatI WGTACW 5 cut(s) 1062, 1118, 1692, 2667, 2908
TauI GCSGC 7 cut(s) 103, 191, 257, 283, 299, 1246, 3548
Tru1I TTAA 8 cut(s) 467, 986, 1433, 1461, 1553, 1749, 3152, 3569
Tru9I TTAA 8 cut(s) 467, 986, 1433, 1461, 1553, 1749, 3152, 3569
TscAI CASTG 2 cut(s) 2316, 3483
TseFI GTSAC 4 cut(s) 160, 203, 214, 2316
Tsp45I GTSAC 4 cut(s) 160, 203, 214, 2316
TspGWI ACGGA 2 cut(s) 281, 1717
TspRI CASTG 2 cut(s) 2316, 3483
Van91I CCANNNNNTGG 2 cut(s) 1961, 2441
VpaK11BI GGWCC 5 cut(s) 382, 1504, 1891, 3332, 3471
XagI CCTNNNNNAGG 1 cut(s) 2303
XapI RAATTY 7 cut(s) 181, 748, 861, 2680, 2923, 2949, 2992
XbaI TCTAGA 1 cut(s) 1531
XceI RCATGY 2 cut(s) 1319, 1766
XcmI CCANNNNNNNNNTGG 1 cut(s) 3070
XmiI GTMKAC 2 cut(s) 1290, 2272
XmnI GAANNNNTTC 5 cut(s) 1359, 2287, 2729, 2851, 3174
XspI CTAG 6 cut(s) 1128, 1532, 1884, 1923, 2004, 2966
ZraI GACGTC 1 cut(s) 214
ZrmI AGTACT 2 cut(s) 2669, 2910
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.