MD06G1222500.v1.1

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0013882
Physical Location & Seq
Forward (+)
35394590 .. 35402539
7950 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1222500.v1.1.491

Sequence Viewer

Length: 3699 bp
ATGCCGTACTCCGCCCTCTTCCAGTGCTATATATTCCGAACCACCACCATGTCCCTCAAACACTCCACCACTTCCACCACCCCCGTTCTCGTCTCTCTACGCGGAACCCACCACCACCACCGCCACCGCCACCACGTAGCTTTGATAAGGCCGTCGCTGCTACTGCGATGCTCCATGATGAACAGCCGGTACCACCACTCGCTCTGCCTTGCTCCGGCCCGGAATTGGAATGCGTGCTATTATCTGCCCCGGTACGCGGGGGACGTCACGTGCCAGGCATCGTCTCGGGCGGCGGAGCTGGATTGGAAGCAGAGGCAGCAGCTGAGGAGTTCGGCGGTGCCGTTTTACCAGCAGAATTTAAGCTATGGGCGGTTTGCGTACCAGGACGCCTCCGCGAGTGAGGAGTCCGATGCCGAATTGGGGTCGTCTCAGCGCCAAAAGGGTGGTTCGACCCTTGATAACATTGATGAATGGAGATGGAAGTTAACCATGCTTCTTCGCAACAAAGACGAGCAAGAATTGGTGTCCAGGGAAAAAAAGGATAGACGCGATTTTGATCATCTTTCAGCACTGGCAAGCAGGATGGGTTTGTATAGCCGTCAATATTCTAGAGTTGTAGTATTTAGCAAAGTTCCTCAGCCCAATTACAGACCTGATCTTGATGATAAGCGGCCACAAAGAGAGGTTGTCTTACCTTTTGGACTTCATAGAGAAGTAGATGCTCATCTCAAAGCCTACCTATCTCAAAAGCCAATGAGCCAAGGAAATTTGTCAGACTTTTCCTTGTCAAGATCAAACAGTAATAGAAGCATTACTAATGATGGAGGTCATTATGAGCAGCAGGAGCCCTTGATACAGAATACAGATGCCATGGAGAAAATTCTTCAGCGGAAAAGCTTGCAACTGCATAATAGGCAACGACATTGGCAGGAATCGCCAGAAGGTCAAAAGATGCTTGAGCTTCGTAAAAGTCTGCCTGCATATAAGGAGAAAGATGCACTGTTAAAAGCTGTATCAGAAAATCAGGTCGTAGTTGTCTCCGGCGAAACAGGTTGTGGTAAAACTACACAACTTCCTCAGTACATTTTAGAATCTGAGATTGAAGCTGATCGCGGAGCTTCCTGCAGTATTATTTGTACCCAGCCTAGACGGATATCTGCCATGGCTGTTTCTGAAAGAGTAGCTGCGGAACGAGGGGAGAGTCTGGGGGAATCTGTTGGTTACAAAGTTAGGCTTGAATGGATGAAAGGGCGGGACACCCGGCTTCTTTTTTGCACCACAGGCATATTATTAAGGAGATTACTTGTTGACAGAAAGTTGAGAGGTGTTACACACGTCATTGTTGACGAGATTCATGAACGGGGAATGAATGAAGATTTTCTACTTATTGTTCTGAAAGAACTTCTCCCTCGCCGGCCAGAGTTGAGGTTGATTTTGATGAGTGCCACCCTAAATGCTGAGCTTTTCTCCTCCTACTTTGGTCGTGCTCCAACGATCCATATTCCCGGGTTTACTTACCCAGTCCGAACACATTTTCTGGAGAACATTCTAGAAATGACTGGGTATCGGTTAAATCAATATAATCAGATTGATGATTATGGTCATGAAAAGTCGTGGAAAATGCAGAAACAAGCTCAAGGTTTAAAAAAGAGGAAGAGCCAAATTGCTTCTGCCGTAGAGGATGTACTTGAAGCTGCCGACTTTAGGGAGTATAGTCCAAGGACTCAGGAGTCTCTGTCCTGTTGGAATCCTGACTCTATTGGTTTTAACCTCATTGAGAGTCTTCTTTTACACATAGTCAGGAAAGAACGGCCTGGCGCAATTTTAGTTTTTATGACGGGTTGGGATGACATAAACTCCTTGAAGGATCAACTCCAAGCTCATCCCCTATTAGGAGATCCTAGCAGGGTCCTACTGCTGGCATGTCATGGTTCCATGCCTAGCTCTGAGCAGAAATTGATATTTGATAAGCCAGAAGATGGTGTTCGGAAAATAGTTCTAGCTACAAACATGGCTGAGACTAGTATCACTATCAATGATGTAGTTTTTGTTGTTGATTGTGGAAAGGCGAAAGAGACATCATATGATGCACTAAATAACACTCCCTGTTTGCTTCCTTCCTGGATTTCAAAGGCTGCTTCTCGGCAAAGAAGAGGCAGAGCTGGTCGTGTTCAACCTGGCGAGTGTTACCATCTCTACCCCAGATGTGTATATGATGCCTTTGCTGATTATCAGTTGCCTGAACTTTTGAGGACTCCATTGCAATCATTATGTTTACAAATCAAAAGTCTACAACTTGGAAGTATTTCAGAGTTCCTATCTAAGGCCTTGCAGCCGCCGGAACCTCTGTCGGTTCAAAATGCTGTTGAGTATTTAAAGATCATTGGGGCTTTAGATGATTATGAGGATCTGACAGTGTTAGGACGTCACCTGTCAGTGCTTCCCGTTGAGCCTAAACTTGGAAAAATGCTCATATTGGGGTCTATTTTCAACTGTTTGGATCCAGTAATGACTGTTGTCGCAGGCCTTAGTATGAGAGACCCATTCCTTATGCCGCATGACAGGAAGGATCTTGCAGAGTCCGCAAAAGCACAGTTCTCTGCTCATGATAACAGTGATCATCTTGCTCTTGTCCGAGCTTATGATGGTTGGAGAAGTGCTGAAAGAACACAATCTGGCTATGAGTACTGCTGGAGAAACTTTCTTTCTGTACAAACTCTGAAAGCCATTGACTCTCTTCGGAAGCAGTTCTTCTTTTTGCTCAAGGATGCAGGTCTGGCTGACCATAATACAGAAAGCTGCAATACATGGAGCCATGATGAGCATCTTGTCCGAGCGGTCATCTGTGCTGGATTATTTCCTGGAATATGCTCTGTCGTGAACAAAGAGAAGTCGATTATGCTTAAAACACAGGAAGATGGACAAGTGATGCTATACTCGAGTTCTGTCAACGGAAGCATACCCAAAATTCCATATCCATGGCTAGTCTTCAACGAAAAGGTGAAAGTGAATTCGGTTTTTCTCCGGGATTCAACTGGTATATCTGATTCTGTGCTCCTTTTGTTCGGGGGCAACATTTCCATGGGTGGCCTGAATGGGCACTTGAAGATGTTGGGAGGATACTTGGAGTTTTTCATGAATCCCACATTAGCAGATACATATCTTTGCTTAAAGAAGGAGCTTGATGAACTGATTCATAATAAGCTCCTGAATCCCAAATTGGATATGCAATCACATACAAACCTCCTATCGGCTCTAAGATTACTGGTCTCAGAGGATCAATGTGAGGGTAGATTTGTATTTGGTCGCAAGGTGCCAGTGCCCTCAATGAAGGCAACACAAGAGAAGTTACCAAACTTAAAAGGCACCACGAAGAAAGTTGCGGAGAACAATAATTACAAAAATCACCTGCAAACATTGCTCACCAGGGCCGGGCATGATATACCCACCTACAAAACAAAACCATTGAAAAACAACCAGTTCCGTTCCACAGTGATCTTCAATGGGTTGAACTTTGTAGGGAAGCCTTGCAATAGTAAGAAAGAAGCAGAGAAAGATGCAGCTGCAGAAGCTGTACTCTGGTTGAAGGGAGAAAACCATTCTTCGTCCACAGATATTGACCATATGTCGATGCTTCTTAAGAAAAGTAGAAAGACAAGCCAGAAAAGGACCTCATTTGATAGTGCCAAGTGGGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001047 GO:0001067 GO:0001503 GO:0001817 GO:0001819 GO:0002151 GO:0002791 GO:0002793 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003697 GO:0003723 GO:0003724 GO:0003725 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006355 GO:0006357 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0008186 GO:0009605 GO:0009607 GO:0009615 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010501 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010638 GO:0014070 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0019899 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031330 GO:0032204 GO:0032206 GO:0032479 GO:0032481 GO:0032501 GO:0032647 GO:0032727 GO:0032879 GO:0032880 GO:0033043 GO:0033044 GO:0034641 GO:0042221 GO:0042623 GO:0042826 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043330 GO:0043331 GO:0043487 GO:0043489 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050707 GO:0050708 GO:0050714 GO:0050715 GO:0050789 GO:0050794 GO:0050896 GO:0051046 GO:0051047 GO:0051049 GO:0051050 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051222 GO:0051223 GO:0051239 GO:0051240 GO:0051252 GO:0051253 GO:0051254 GO:0051704 GO:0051707 GO:0051880 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070035 GO:0070201 GO:0071704 GO:0080090 GO:0090087 GO:0090304 GO:0090669 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1902369 GO:1902680 GO:1902739 GO:1902741 GO:1903506 GO:1903508 GO:1903530 GO:1903532 GO:1904951 GO:1990837 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind dsrm

Protein Analysis

1233

Amino Acids

139.73

Weight (kDa)

8.98

Isoelectric Point (pI)

45.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 335 - 487 2.7e-11 DEAD/DEAH box helicase
Helicase_C PF00271 595 - 724 3.4e-12 Helicase conserved C-terminal domain
WHD_HA2 PF04408 788 - 816 8.5e-08 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 817 - 877 2.2e-14 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 944 - 1026 8.3e-19 Oligonucleotide/oligosaccharide-binding (OB)-fold
RNA_hel_CTD PF26026 1034 - 1092 8.4e-20 C-terminal domain in RNA helicases
dsrm PF00035 1133 - 1195 3.4e-09 Double-stranded RNA binding motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013882)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G48650 AT1G48650
fragaria_vesca FvH4_5g16860
malus_domestica MD06G1222500.v1.1 MD14G1231600.v1.1
prunus_persica Prupe.5G227100_v2.0.a1
pyrus_communis pycom06g19810 pycom06g19820 pycom14g19280
rosa_chinensis RchiOBHm_Chr7g0179211
rosa_laevigata RLG00000005374
rosa_multiflora Rmu_sc0009798.1_g000005 Rmu_sc0021799.1_g000001
rosa_roxburghii Rroxscaffold_3G00274220
rosa_rugosa Rorug06G0422200
rosa_samantha Rh7BG021500
rosa_wichuraiana Rw7G001770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3417
AasI GACNNNNNNGTC 1 cut(s) 1729
AatII GACGTC 2 cut(s) 267, 2428
Acc36I ACCTGC 2 cut(s) 2762, 3417
Acc65I GGTACC 1 cut(s) 189
AccB1I GGYRCC 4 cut(s) 189, 337, 3313, 3365
AccB7I CCANNNNNTGG 1 cut(s) 1979
AccBSI CCGCTC 1 cut(s) 2837
AccI GTMKAC 1 cut(s) 2290
AccII CGCG 5 cut(s) 102, 257, 395, 549, 1113
AclWI GGATC 8 cut(s) 1489, 1875, 1892, 2415, 2495, 2508, 2577, 3285
AcoI YGGCCR 2 cut(s) 671, 1415
AcsI RAATTY 5 cut(s) 355, 766, 879, 2967, 3010
AcuI CTGAAG 1 cut(s) 869
AcvI CACGTG 1 cut(s) 270
AcyI GRCGYC 3 cut(s) 264, 387, 2425
AflII CTTAAG 1 cut(s) 3638
AflIII ACRYGT 1 cut(s) 1333
AhdI GACNNNNNGTC 1 cut(s) 3625
AhlI ACTAGT 1 cut(s) 2021
AjiI CACGTC 1 cut(s) 1336
AjnI CCWGG 8 cut(s) 273, 381, 527, 1813, 2120, 2176, 2860, 3425
AjuI GAANNNNNNNTTGG 2 cut(s) 3203, 3235
Alw21I GWGCWC 2 cut(s) 1489, 3057
Alw26I GTCTC 9 cut(s) 97, 288, 432, 1042, 1737, 2012, 2069, 2532, 3274
AlwI GGATC 8 cut(s) 1489, 1875, 1892, 2415, 2495, 2508, 2577, 3285
AlwNI CAGNNNCTG 2 cut(s) 322, 3572
Ama87I CYCGRG 3 cut(s) 285, 1505, 2938
AoxI GGCC 9 cut(s) 149, 216, 671, 1415, 1811, 2325, 2524, 3088, 3429
ApoI RAATTY 5 cut(s) 355, 766, 879, 2967, 3010
ArsI GACNNNNNNTTYG 2 cut(s) 2272, 2304
Asp700I GAANNNNTTC 4 cut(s) 1377, 2305, 2747, 3192
Asp718I GGTACC 1 cut(s) 189
AspLEI GCGC 2 cut(s) 435, 1820
AspS9I GGNCC 4 cut(s) 217, 1909, 3429, 3669
AsuC2I CCSGG 7 cut(s) 220, 250, 1261, 1506, 1507, 3026, 3433
AsuHPI GGTGA 4 cut(s) 2420, 3013, 3398, 3415
AvaI CYCGRG 3 cut(s) 285, 1505, 2938
AvaII GGWCC 2 cut(s) 1909, 3669
BaeGI GKGCMC 2 cut(s) 3102, 3324
BamHI GGATCC 1 cut(s) 2500
BanI GGYRCC 4 cut(s) 189, 337, 3313, 3365
BanII GRGCYC 1 cut(s) 849
BarI GAAGNNNNNNTAC 2 cut(s) 1365, 1397
BbrPI CACGTG 1 cut(s) 270
BbsI GAAGAC 2 cut(s) 1775, 2980
Bbv12I GWGCWC 2 cut(s) 1489, 3057
BbvCI CCTCAGC 2 cut(s) 323, 636
BccI CCATC 7 cut(s) 471, 577, 815, 1973, 2199, 2639, 2912
BceAI ACGGC 5 cut(s) 136, 325, 582, 1658, 1826
BciT130I CCWGG 8 cut(s) 275, 383, 529, 1815, 2122, 2178, 2862, 3427
BciVI GTATCC 1 cut(s) 3113
BclI TGATCA 2 cut(s) 556, 2617
BcnI CCSGG 7 cut(s) 220, 250, 1261, 1506, 1507, 3026, 3433
BcoDI GTCTC 9 cut(s) 97, 288, 432, 1042, 1737, 2012, 2069, 2532, 3274
BcuI ACTAGT 1 cut(s) 2021
BfaI CTAG 8 cut(s) 609, 1146, 1550, 1902, 1941, 2000, 2022, 2984
BfmI CTRYAG 2 cut(s) 1123, 3564
BfoI RGCGCY 1 cut(s) 436
BfrI CTTAAG 1 cut(s) 3638
BfuAI ACCTGC 2 cut(s) 2762, 3417
BfuI GTATCC 1 cut(s) 3113
BlpI GCTNAGC 1 cut(s) 1458
BmcAI AGTACT 1 cut(s) 2687
Bme18I GGWCC 2 cut(s) 1909, 3669
BmeRI GACNNNNNGTC 1 cut(s) 3625
BmeT110I CYCGRG 3 cut(s) 285, 1505, 2938
BmgBI CACGTC 1 cut(s) 1336
BmgT120I GGNCC 4 cut(s) 217, 1909, 3429, 3669
BmrI ACTGGG 2 cut(s) 1514, 1569
BmuI ACTGGG 2 cut(s) 1514, 1569
BoxI GACNNNNGTC 1 cut(s) 2516
BpiI GAAGAC 2 cut(s) 1775, 2980
BplI GAGNNNNNCTC 2 cut(s) 1451, 1483
BpmI CTGGAG 2 cut(s) 1559, 2713
Bpu10I CCTNAGC 2 cut(s) 323, 636
Bpu1102I GCTNAGC 1 cut(s) 1458
BpuEI CTTGAG 3 cut(s) 977, 1620, 2747
BpuMI CCSGG 7 cut(s) 220, 250, 1261, 1506, 1507, 3026, 3433
BsaAI YACGTR 2 cut(s) 136, 270
BsaBI GATNNNNATC 4 cut(s) 555, 723, 2823, 3159
BsaHI GRCGYC 3 cut(s) 264, 387, 2425
BsaI GGTCTC 2 cut(s) 2532, 3274
BsaXI ACNNNNNCTCC 4 cut(s) 1721, 1751, 1841, 1871
Bse118I RCCGGY 2 cut(s) 186, 1413
Bse1I ACTGG 9 cut(s) 22, 576, 1520, 1564, 2504, 3040, 3270, 3317, 3478
Bse3DI GCAATG 2 cut(s) 2258, 3416
Bse8I GATNNNNATC 4 cut(s) 555, 723, 2823, 3159
BseBI CCWGG 8 cut(s) 275, 383, 529, 1815, 2122, 2178, 2862, 3427
BseGI GGATG 6 cut(s) 588, 1248, 1687, 1852, 1882, 2773
BseJI GATNNNNATC 4 cut(s) 555, 723, 2823, 3159
BseMI GCAATG 2 cut(s) 2258, 3416
BseNI ACTGG 9 cut(s) 22, 576, 1520, 1564, 2504, 3040, 3270, 3317, 3478
BseRI GAGGAG 3 cut(s) 340, 416, 1459
BseSI GKGCMC 2 cut(s) 3102, 3324
BseYI CCCAGC 1 cut(s) 1140
Bsh1236I CGCG 5 cut(s) 102, 257, 395, 549, 1113
BshFI GGCC 9 cut(s) 151, 218, 673, 1417, 1813, 2327, 2526, 3090, 3431
BshNI GGYRCC 4 cut(s) 189, 337, 3313, 3365
BsiHKAI GWGCWC 2 cut(s) 1489, 3057
BsiHKCI CYCGRG 3 cut(s) 285, 1505, 2938
BslFI GGGAC 3 cut(s) 37, 275, 1268
BsmAI GTCTC 9 cut(s) 97, 288, 432, 1042, 1737, 2012, 2069, 2532, 3274
BsmBI CGTCTC 3 cut(s) 97, 288, 432
BsmFI GGGAC 3 cut(s) 37, 275, 1268
BsmI GAATGC 1 cut(s) 235
BsnI GGCC 9 cut(s) 151, 218, 673, 1417, 1813, 2327, 2526, 3090, 3431
Bso31I GGTCTC 2 cut(s) 2532, 3274
BsoBI CYCGRG 3 cut(s) 285, 1505, 2938
Bsp1286I GDGCHC 5 cut(s) 849, 1489, 3057, 3102, 3324
Bsp1407I TGTACA 1 cut(s) 2710
Bsp1720I GCTNAGC 1 cut(s) 1458
Bsp19I CCATGG 4 cut(s) 870, 1161, 2978, 3081
BspANI GGCC 9 cut(s) 151, 218, 673, 1417, 1813, 2327, 2526, 3090, 3431
BspFNI CGCG 5 cut(s) 102, 257, 395, 549, 1113
BspHI TCATGA 4 cut(s) 1354, 1603, 2605, 3135
BspMAI CTGCAG 2 cut(s) 1127, 3568
BspMI ACCTGC 2 cut(s) 2762, 3417
BspPI GGATC 8 cut(s) 1489, 1875, 1892, 2415, 2495, 2508, 2577, 3285
BspQI GCTCTTC 1 cut(s) 1649
BspT107I GGYRCC 4 cut(s) 189, 337, 3313, 3365
BspTI CTTAAG 1 cut(s) 3638
BspTNI GGTCTC 2 cut(s) 2532, 3274
BsrBI CCGCTC 1 cut(s) 2837
BsrDI GCAATG 2 cut(s) 2258, 3416
BsrFI RCCGGY 2 cut(s) 186, 1413
BsrGI TGTACA 1 cut(s) 2710
BsrI ACTGG 9 cut(s) 22, 576, 1520, 1564, 2504, 3040, 3270, 3317, 3478
BssAI RCCGGY 2 cut(s) 186, 1413
BssNI GRCGYC 3 cut(s) 264, 387, 2425
BssT1I CCWWGG 6 cut(s) 760, 870, 1161, 1718, 2978, 3081
Bst2UI CCWGG 8 cut(s) 275, 383, 529, 1815, 2122, 2178, 2862, 3427
Bst4CI ACNGT 8 cut(s) 800, 1002, 2416, 2495, 2515, 2595, 2615, 3493
Bst6I CTCTTC 4 cut(s) 23, 1649, 2146, 2742
BstACI GRCGYC 3 cut(s) 264, 387, 2425
BstAFI CTTAAG 1 cut(s) 3638
BstAPI GCANNNNNTGC 1 cut(s) 3418
BstAUI TGTACA 1 cut(s) 2710
BstBAI YACGTR 2 cut(s) 136, 270
BstC8I GCNNGC 7 cut(s) 235, 577, 899, 978, 1415, 1920, 2524
BstDSI CCRYGG 4 cut(s) 870, 1161, 2978, 3081
BstENI CCTNNNNNAGG 1 cut(s) 2321
BstF5I GGATG 6 cut(s) 588, 1248, 1687, 1852, 1882, 2773
BstFNI CGCG 5 cut(s) 102, 257, 395, 549, 1113
BstH2I RGCGCY 1 cut(s) 436
BstHHI GCGC 2 cut(s) 435, 1820
BstMAI GTCTC 9 cut(s) 97, 288, 432, 1042, 1737, 2012, 2069, 2532, 3274
BstNI CCWGG 8 cut(s) 275, 383, 529, 1815, 2122, 2178, 2862, 3427
BstNSI RCATGY 1 cut(s) 1926
BstPAI GACNNNNGTC 1 cut(s) 2516
BstSFI CTRYAG 2 cut(s) 1123, 3564
BstSLI GKGCMC 2 cut(s) 3102, 3324
BstUI CGCG 5 cut(s) 102, 257, 395, 549, 1113
BstV2I GAAGAC 2 cut(s) 1775, 2980
BstX2I RGATCY 4 cut(s) 1897, 2407, 2500, 2569
BstXI CCANNNNNNTGG 2 cut(s) 443, 2979
BstYI RGATCY 4 cut(s) 1897, 2407, 2500, 2569
BsuI GTATCC 1 cut(s) 3113
BsuRI GGCC 9 cut(s) 151, 218, 673, 1417, 1813, 2327, 2526, 3090, 3431
BtgI CCRYGG 4 cut(s) 870, 1161, 2978, 3081
BtgZI GCGATG 1 cut(s) 181
BtrI CACGTC 1 cut(s) 1336
BtsCI GGATG 6 cut(s) 588, 1248, 1687, 1852, 1882, 2773
BtsIMutI CAGTG 8 cut(s) 29, 569, 998, 2421, 2442, 2620, 3324, 3498
BveI ACCTGC 2 cut(s) 2762, 3417
Cac8I GCNNGC 7 cut(s) 235, 577, 899, 978, 1415, 1920, 2524
CaiI CAGNNNCTG 2 cut(s) 322, 3572
CciI TCATGA 4 cut(s) 1354, 1603, 2605, 3135
CfoI GCGC 2 cut(s) 435, 1820
Cfr10I RCCGGY 2 cut(s) 186, 1413
Cfr13I GGNCC 4 cut(s) 217, 1909, 3429, 3669
Cfr9I CCCGGG 1 cut(s) 1505
CseI GACGC 2 cut(s) 395, 555
CspCI CAANNNNNGTGG 2 cut(s) 3598, 3633
DraI TTTAAA 2 cut(s) 1644, 2376
DrdI GACNNNNNNGTC 1 cut(s) 1729
DriI GACNNNNNGTC 1 cut(s) 3625
DseDI GACNNNNNNGTC 1 cut(s) 1729
EaeI YGGCCR 2 cut(s) 671, 1415
Eam1104I CTCTTC 4 cut(s) 23, 1649, 2146, 2742
Eam1105I GACNNNNNGTC 1 cut(s) 3625
EarI CTCTTC 4 cut(s) 23, 1649, 2146, 2742
EciI GGCGGA 1 cut(s) 308
Eco130I CCWWGG 6 cut(s) 760, 870, 1161, 1718, 2978, 3081
Eco147I AGGCCT 2 cut(s) 2327, 2526
Eco24I GRGCYC 1 cut(s) 849
Eco31I GGTCTC 2 cut(s) 2532, 3274
Eco32I GATATC 1 cut(s) 1155
Eco47I GGWCC 2 cut(s) 1909, 3669
Eco57I CTGAAG 1 cut(s) 869
Eco72I CACGTG 1 cut(s) 270
Eco88I CYCGRG 3 cut(s) 285, 1505, 2938
EcoNI CCTNNNNNAGG 1 cut(s) 2321
EcoO109I RGGNCCY 2 cut(s) 1909, 3669
EcoRI GAATTC 1 cut(s) 3010
EcoRII CCWGG 8 cut(s) 273, 381, 527, 1813, 2120, 2176, 2860, 3425
EcoRV GATATC 1 cut(s) 1155
EcoT14I CCWWGG 6 cut(s) 760, 870, 1161, 1718, 2978, 3081
EcoT38I GRGCYC 1 cut(s) 849
ErhI CCWWGG 6 cut(s) 760, 870, 1161, 1718, 2978, 3081
Esp3I CGTCTC 3 cut(s) 97, 288, 432
FalI AAGNNNNNCTT 4 cut(s) 1218, 1250, 2735, 2767
FaqI GGGAC 3 cut(s) 37, 275, 1268
FauI CCCGC 2 cut(s) 250, 1245
FauNDI CATATG 2 cut(s) 2083, 3624
FbaI TGATCA 2 cut(s) 556, 2617
FblI GTMKAC 1 cut(s) 2290
FokI GGATG 6 cut(s) 595, 1255, 1694, 1859, 1869, 2780
FriOI GRGCYC 1 cut(s) 849
FspBI CTAG 8 cut(s) 609, 1146, 1550, 1902, 1941, 2000, 2022, 2984
GlaI GCGC 2 cut(s) 434, 1819
GsaI CCCAGC 1 cut(s) 1144
GsuI CTGGAG 2 cut(s) 1559, 2713
HaeII RGCGCY 1 cut(s) 436
HaeIII GGCC 9 cut(s) 151, 218, 673, 1417, 1813, 2327, 2526, 3090, 3431
HgaI GACGC 2 cut(s) 395, 555
HhaI GCGC 2 cut(s) 435, 1820
Hin1I GRCGYC 3 cut(s) 264, 387, 2425
Hin6I GCGC 2 cut(s) 433, 1818
HinP1I GCGC 2 cut(s) 433, 1818
HincII GTYRAC 4 cut(s) 486, 1309, 1345, 2950
HindII GTYRAC 4 cut(s) 486, 1309, 1345, 2950
HindIII AAGCTT 1 cut(s) 895
HpaI GTTAAC 1 cut(s) 486
HphI GGTGA 4 cut(s) 2420, 3013, 3398, 3415
Hpy166II GTNNAC 9 cut(s) 486, 1309, 1345, 1512, 2276, 2291, 2881, 2950, 3609
Hpy8I GTNNAC 9 cut(s) 486, 1309, 1345, 1512, 2276, 2291, 2881, 2950, 3609
Hpy99I CGWCG 1 cut(s) 157
HpyAV CCTTC 7 cut(s) 935, 1858, 2127, 2560, 3169, 3325, 3580
HpyCH4III ACNGT 8 cut(s) 800, 1002, 2416, 2495, 2515, 2595, 2615, 3493
HpyCH4IV ACGT 5 cut(s) 135, 264, 269, 1335, 2425
HpySE526I ACGT 5 cut(s) 135, 264, 269, 1335, 2425
Hsp92I GRCGYC 3 cut(s) 264, 387, 2425
HspAI GCGC 2 cut(s) 433, 1818
KpnI GGTACC 1 cut(s) 193
KroI GCCGGC 1 cut(s) 1413
KroNI GCCGGC 1 cut(s) 1415
Ksp22I TGATCA 2 cut(s) 556, 2617
KspAI GTTAAC 1 cut(s) 486
LguI GCTCTTC 1 cut(s) 1649
MaeI CTAG 8 cut(s) 609, 1146, 1550, 1902, 1941, 2000, 2022, 2984
MaeII ACGT 5 cut(s) 135, 264, 269, 1335, 2425
MaeIII GTNAC 6 cut(s) 265, 1220, 1327, 2186, 2426, 3348
MbiI CCGCTC 1 cut(s) 2837
MflI RGATCY 4 cut(s) 1897, 2407, 2500, 2569
MhlI GDGCHC 5 cut(s) 849, 1489, 3057, 3102, 3324
MlyI GAGTC 9 cut(s) 413, 1210, 1717, 1739, 1748, 1789, 2248, 2588, 2726
MmeI TCCRAC 3 cut(s) 1514, 1724, 2630
MroNI GCCGGC 1 cut(s) 1413
MroXI GAANNNNTTC 4 cut(s) 1377, 2305, 2747, 3192
MslI CAYNNNNRTG 3 cut(s) 47, 2977, 3080
MspA1I CMGCKG 3 cut(s) 322, 889, 3563
MspCI CTTAAG 1 cut(s) 3638
Mva1269I GAATGC 1 cut(s) 235
MvaI CCWGG 8 cut(s) 275, 383, 529, 1815, 2122, 2178, 2862, 3427
MvnI CGCG 5 cut(s) 102, 257, 395, 549, 1113
NaeI GCCGGC 1 cut(s) 1415
NciI CCSGG 7 cut(s) 220, 250, 1261, 1506, 1507, 3026, 3433
NcoI CCATGG 4 cut(s) 870, 1161, 2978, 3081
NdeI CATATG 2 cut(s) 2083, 3624
NgoMIV GCCGGC 1 cut(s) 1413
NmeAIII GCCGAG 1 cut(s) 2122
NmuCI GTSAC 2 cut(s) 265, 2426
NspI RCATGY 1 cut(s) 1926
PaeR7I CTCGAG 1 cut(s) 2938
PagI TCATGA 4 cut(s) 1354, 1603, 2605, 3135
PaqCI CACCTGC 1 cut(s) 3417
PceI AGGCCT 2 cut(s) 2327, 2526
PciSI GCTCTTC 1 cut(s) 1649
PcsI WCGNNNNNNNCGW 2 cut(s) 261, 338
PctI GAATGC 1 cut(s) 235
PdiI GCCGGC 1 cut(s) 1415
PdmI GAANNNNTTC 4 cut(s) 1377, 2305, 2747, 3192
PflMI CCANNNNNTGG 1 cut(s) 1979
PfoI TCCNGGA 3 cut(s) 2120, 2860, 3024
PleI GAGTC 9 cut(s) 412, 1209, 1717, 1738, 1748, 1788, 2248, 2587, 2726
PmaCI CACGTG 1 cut(s) 270
PmlI CACGTG 1 cut(s) 270
PpsI GAGTC 9 cut(s) 412, 1209, 1717, 1738, 1748, 1788, 2248, 2587, 2726
Ppu21I YACGTR 2 cut(s) 136, 270
PpuMI RGGWCCY 2 cut(s) 1909, 3669
PshAI GACNNNNGTC 1 cut(s) 2516
Psp5II RGGWCCY 2 cut(s) 1909, 3669
Psp6I CCWGG 8 cut(s) 273, 381, 527, 1813, 2120, 2176, 2860, 3425
PspCI CACGTG 1 cut(s) 270
PspFI CCCAGC 1 cut(s) 1140
PspGI CCWGG 8 cut(s) 273, 381, 527, 1813, 2120, 2176, 2860, 3425
PspPI GGNCC 4 cut(s) 217, 1909, 3429, 3669
PspPPI RGGWCCY 2 cut(s) 1909, 3669
PspXI VCTCGAGB 1 cut(s) 2938
PsrI GAACNNNNNNTAC 4 cut(s) 173, 205, 3380, 3412
PstI CTGCAG 2 cut(s) 1127, 3568
PstNI CAGNNNCTG 2 cut(s) 322, 3572
PsuI RGATCY 4 cut(s) 1897, 2407, 2500, 2569
PvuII CAGCTG 2 cut(s) 322, 3563
RseI CAYNNNNRTG 3 cut(s) 47, 2977, 3080
SapI GCTCTTC 1 cut(s) 1649
Sau96I GGNCC 4 cut(s) 217, 1909, 3429, 3669
ScaI AGTACT 1 cut(s) 2687
SchI GAGTC 9 cut(s) 413, 1210, 1717, 1739, 1748, 1789, 2248, 2588, 2726
SduI GDGCHC 5 cut(s) 849, 1489, 3057, 3102, 3324
SfcI CTRYAG 2 cut(s) 1123, 3564
Sfr274I CTCGAG 1 cut(s) 2938
SinI GGWCC 2 cut(s) 1909, 3669
SlaI CTCGAG 1 cut(s) 2938
SmaI CCCGGG 1 cut(s) 1507
SmiMI CAYNNNNRTG 3 cut(s) 47, 2977, 3080
SmlI CTYRAG 5 cut(s) 956, 1635, 2762, 2938, 3638
SmoI CTYRAG 5 cut(s) 956, 1635, 2762, 2938, 3638
SpeI ACTAGT 1 cut(s) 2021
SseBI AGGCCT 2 cut(s) 2327, 2526
SspI AATATT 1 cut(s) 605
SspMI CTAG 8 cut(s) 609, 1146, 1550, 1902, 1941, 2000, 2022, 2984
StuI AGGCCT 2 cut(s) 2327, 2526
StyI CCWWGG 6 cut(s) 760, 870, 1161, 1718, 2978, 3081
TaaI ACNGT 8 cut(s) 800, 1002, 2416, 2495, 2515, 2595, 2615, 3493
TaiI ACGT 5 cut(s) 138, 267, 272, 1338, 2428
TaqI TCGA 4 cut(s) 449, 2894, 2939, 3629
TatI WGTACW 5 cut(s) 1080, 1684, 2685, 2710, 3574
TauI GCSGC 4 cut(s) 293, 673, 2338, 2557
TscAI CASTG 8 cut(s) 29, 576, 1005, 2421, 2442, 2620, 3324, 3498
TseFI GTSAC 2 cut(s) 265, 2426
Tsp45I GTSAC 2 cut(s) 265, 2426
TspGWI ACGGA 3 cut(s) 1165, 2967, 3473
TspMI CCCGGG 1 cut(s) 1505
TspRI CASTG 8 cut(s) 29, 576, 1005, 2421, 2442, 2620, 3324, 3498
Van91I CCANNNNNTGG 1 cut(s) 1979
Vha464I CTTAAG 1 cut(s) 3638
VpaK11BI GGWCC 2 cut(s) 1909, 3669
XagI CCTNNNNNAGG 1 cut(s) 2321
XapI RAATTY 5 cut(s) 355, 766, 879, 2967, 3010
XbaI TCTAGA 2 cut(s) 608, 1549
XceI RCATGY 1 cut(s) 1926
XhoI CTCGAG 1 cut(s) 2938
XmaI CCCGGG 1 cut(s) 1505
XmiI GTMKAC 1 cut(s) 2290
XmnI GAANNNNTTC 4 cut(s) 1377, 2305, 2747, 3192
XspI CTAG 8 cut(s) 609, 1146, 1550, 1902, 1941, 2000, 2022, 2984
ZraI GACGTC 2 cut(s) 265, 2426
ZrmI AGTACT 1 cut(s) 2687
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.