MD14G1231600.v1.1

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0013882
Physical Location & Seq
Forward (+)
31207096 .. 31215170
8075 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1231600.v1.1.491

Sequence Viewer

Length: 3705 bp
ATGCCGTACTCCGCCCTCTACCAGGGATATATATTCAGGACCACCACCATGTCCCTCAAACCCTCCACCACTTCCACCACTACCGTTCTCGTCTCTCTCCGCGGGACCCCCCACCGCCGCCACCACGTAGCTTTGGTAAGGCCGTCGATGCTGCTGGGATGCTCCATGAGGAACAGCCGGTACCACCACACGCACTGCCTTGCTCCGCCGCTGAATTGGAATGCGTGCTATTATCTGCCGCGTCATGTGGGGGACGTCGCGTGCCAGGCTTCGTCTCGGGCGGCGGAGCTGGATTGGAAGCAGAGGAAGCAGCTGAGGAGTTCCGCGGTGCCGTTTTACCAGCAGAATTTGAGCTATGGGCGGTTTGCCTACCAGGACGCCTCCGCGAGTGAGGAGTCCGACGCCGAATTGGGGTCGTCTCAGCGCCAAATGGGTGGTTCGACCCTTGATAACATTGACGAATGGAGATGGAAGTTAACCATGCTTCTTCGCAACAAAGATGAGCAAGAATTGGTGTCCAGGGAAAGAAAGGATAGACGCGATTTTGATCATCTTTCAGCACTGGCAAGTAGGATGGGTTTATATAGCCGTCAGTATTCTAGAGTTGTAGTATTTAGCAAAATTCCTCAGCCAAATTACAGACCTGATCTGGACGATAAGCGGCCACAAAGAGAGGTTGTCTTACCTTTTGGACTTCATAGAGAAGTAGATGCTCATCTCAAAGCCTACCTATCTGAAAAGCCAATGAGCCAAGGAAATGTATCAGATTCTTCCTTGTCAAGATCAAACAGTAGTAGAAGCATTACTAATGATGGAGGACATTATGAGCAGCAGGAGCCCTTGATACAGAATACTGATGCCATGGAGAAAATTCTTCAGCGGAAAAGCTTGCAACTGCGTAATAGGCAACGACATTGGCAGGAATCTCCAGAAGGTCAAAAGATGCTTGAACTTCGTAAAAGTCTGCCTGCATACAAGAAGAAAGATGCACTGTTAAAAGCTGTGTCAGAAAATCAGGTCTTAGTTGTCTCAGGCGAAACTGGTTGTGGTAAAACTACGCAGCTTCCTCAGTACATTTTAGAATCTGAGATTGAAGCTGATCGCGGAGCTTCCTGCAGTATTATTTGTACCCAGCCTAGACGGATATCTGCCATGGCTGTTTCTGAAAGAGTTGCTGCAGAACGAGGGGAGAATCTGGGGGAATCTGTTGGTTACAAAGTTAGGCTTGAAGGGATGAAAGGGCGGGACACCCGGCTTCTTTTTTGCACCACGGGTATATTATTGAGGAGATTACTTGTTGACAGAAAGTTGAGAGGTGTTACACATGTCGTTGTTGATGAGATTCATGAACGCGGAATGAATGAAGATTTTCTTCTTATTGTTCTGAAAGAACTTCTCCCTCGCCGGCCAGAGTTGAGGTTGATTTTGATGAGTGCCACGCTAAATGCTGAGCTTTTCTCCTCCTACTTTGGTGGTGCTCCAATGATCCATATTCCCGGGTTTACATACCCAGTCCGAGCACATTTTCTGGAGAATATTCTAGAAATGACTGGGTATCAGTTGAATCAATATAATCAGATTGATGATTATGGTCATGAAAAGTCGTGGAAAATGCAGAAACAAGCTCAAGGTTTCAAAAAGAGGAAGAGCCAAATTGCTTCTAGTGTTGAGGATGTACTTGAAGCTGCTGACTTTAGGGAGTACAGTCCAAGGACTCAGGAGTCTTTGTCCTATTGGAATCCTGACTCCATTGGTTTTAACCTCATTGGGAATCTTCTTTTACACATAGTCAAGAAAGAACGGCCTGGTGCAATTTTGGTTTTTATGACGGGTTGGGATGACATAAATTCCTTGAAGGATCAACTCCAAGCTCATCCCCTATTAGGAGATCCTAGCAGGGTCCTACTGCTAGCATGTCATGGTTCCATGCCTAGCTCTGAGCAGAAATTGATATTTGATAAGCCAGAAGATGGTGTTCGGAAAATAGTTCTAGCTACAAACATGGCTGAGACTAGTATCACTATCAATGATGTAGTTTTTGTTGTTGATTGTGGAAAGGCGAAAGAGACATCATATGATGCACTAAATAACACTCCCTGTTTGCTTCCATCCTGGATTTCAAAGGCTGCTTCTCGGCAAAGGAGAGGTAGAGCTGGTCGTGTTCAACCTGGTGAGTGTTACCATCTCTACCCCAGATGTGTATATAATGCCTTTGCTGATTATCAGTTGCCTGAACTTTTGAGGACTCCCTTGCAATCTTTATGTTTACAAATCAAAAGTCTACAACTTGGAAGTATTTCAGAGTTCCTATCTAAGGCCTTGCAGCCACCTGAACCTCTGTCGGTTCAAAATGCTGTTGAGTATTTGAAGATCATTGGGGCTTTAGATGATAGTGAAGATCTGACAGTGTTAGGACATCACCTGTCAGTGCTTCCCGTTGAGCCTAAACTTGGAAAAATGCTCATATTAGGGTGTATTTTCAACTGTCTGGATCCAGTAATGACTGTTGTCGCAGGCCTCAGTATGAGAGATCCATTCCTTATGCCGCATGACAAGAAGGATCTTGCAGAGTCGGCAAAAGCACAGTTCTCTGCTCGTGAAAACAGCGATCATCTTGCTCTCGTCCGAGCTTATGATGGTTGGAGAAGTGCTGAAAGAACAGAGTCTGGCTATGAGTACTGCTGGAGAAACTTCCTTTCTGTGCAAACTCTGAAAGCCATTGACTCTCTTCGGAAGCAGTTCTTCTTTTTGCTCAAGGATGCGGGTCTGGTTGACCATAATACAGAAAACTGCAATACATGGAGCCATGATGAGCATCTTGTCCGAGCAGTCATCTGTGCTGGATTATTTCCGGGAATATGCTCTGTCGTGAACAAAGAGAAGTCAATTATGCTGAAAACACAGGAAGATGGACAAGTGATGCTATACTCGAGTTCTGTCAATGGAAACATACCCAAAATTCCATATCCATGGCTTGTCTTCAACGAAAAGGTGAAAGTGAATTCAGTTTTTCTCCGGGATTCAACTGGTATATCTGATTCTGTGCTCCTTTTGTTCGGAGGCAACATTTCCAGGGGTGGCGTGGATGGGCACTTGAAGATGCTGGGAGGATACTTAGAGTTTTTCATGAATCCCGCATTAGCAGAAACATATCTTTGCTTAAAGAGGGAGCTTGATGAACTGATACATAATAAGCTCCTGAATCCCAAATTGGATATGCAATCACACACGAGCCTCCTATCAGCGCTAAGATTACTGGTCTCAGAGGATCAATGTGAGGGTAGATTTGTATTTGGCCGCAAGGTGCCAGTGCCCTCAAAGAAGGCAACACAAGAGAAGGTACCAAAGTTAAAAGGCACCATGAAGAAAGTTGCGGAGAACAATAATTACAAAAATCACCTCCAAACATTGCTCACCAGAGCCGGGCATGATGCACCCACCTACAAAACAAAACCATCGAAAAACAACCAGTTCCGTTCCACAGTGATCTTCAATGGGTTGAACTTTGTAGGCAAACCTTGCAATAGTAAGAAAGAAGCAGAGAAGGACGCAGCTGCTGAAGCTGTACTCTGGTTAAAGGGAGAGAATCATTCATCGTCCACAGGTATTGACCATATGTCGATGCTGCTTAAGAAAAGTAGAAAGAAAAGCCAGAAAAGGACCTCATTTGATAGTGCCAATGGGGTTAAGGGTTGCTCGTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001047 GO:0001067 GO:0001503 GO:0001817 GO:0001819 GO:0002151 GO:0002791 GO:0002793 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003697 GO:0003723 GO:0003724 GO:0003725 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006355 GO:0006357 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0008186 GO:0009605 GO:0009607 GO:0009615 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010501 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010638 GO:0014070 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0019899 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031330 GO:0032204 GO:0032206 GO:0032479 GO:0032481 GO:0032501 GO:0032647 GO:0032727 GO:0032879 GO:0032880 GO:0033043 GO:0033044 GO:0034641 GO:0042221 GO:0042623 GO:0042826 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043330 GO:0043331 GO:0043487 GO:0043489 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050707 GO:0050708 GO:0050714 GO:0050715 GO:0050789 GO:0050794 GO:0050896 GO:0051046 GO:0051047 GO:0051049 GO:0051050 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051222 GO:0051223 GO:0051239 GO:0051240 GO:0051252 GO:0051253 GO:0051254 GO:0051704 GO:0051707 GO:0051880 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070035 GO:0070201 GO:0071704 GO:0080090 GO:0090087 GO:0090304 GO:0090669 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1902369 GO:1902680 GO:1902739 GO:1902741 GO:1903506 GO:1903508 GO:1903530 GO:1903532 GO:1904951 GO:1990837 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind dsrm

Protein Analysis

1235

Amino Acids

139.2

Weight (kDa)

9.08

Isoelectric Point (pI)

46.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 331 - 484 1.4e-10 DEAD/DEAH box helicase
Helicase_C PF00271 592 - 721 3.2e-12 Helicase conserved C-terminal domain
WHD_HA2 PF04408 785 - 813 2.4e-08 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 814 - 874 1.3e-14 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 941 - 1023 4.4e-19 Oligonucleotide/oligosaccharide-binding (OB)-fold
RNA_hel_CTD PF26026 1031 - 1089 4e-20 C-terminal domain in RNA helicases
dsrm PF00035 1130 - 1192 1.3e-09 Double-stranded RNA binding motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013882)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G48650 AT1G48650
fragaria_vesca FvH4_5g16860
malus_domestica MD06G1222500.v1.1 MD14G1231600.v1.1
prunus_persica Prupe.5G227100_v2.0.a1
pyrus_communis pycom06g19810 pycom06g19820 pycom14g19280
rosa_chinensis RchiOBHm_Chr7g0179211
rosa_laevigata RLG00000005374
rosa_multiflora Rmu_sc0009798.1_g000005 Rmu_sc0021799.1_g000001
rosa_roxburghii Rroxscaffold_3G00274220
rosa_rugosa Rorug06G0422200
rosa_samantha Rh7BG021500
rosa_wichuraiana Rw7G001770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1720
AatII GACGTC 1 cut(s) 258
Acc65I GGTACC 2 cut(s) 180, 3340
AccB1I GGYRCC 5 cut(s) 180, 328, 3304, 3340, 3356
AccB7I CCANNNNNTGG 1 cut(s) 1970
AccI GTMKAC 1 cut(s) 2281
AccII CGCG 8 cut(s) 102, 241, 260, 326, 386, 540, 1104, 1353
AclWI GGATC 8 cut(s) 1480, 1866, 1883, 2486, 2499, 2525, 2568, 3276
AcoI YGGCCR 3 cut(s) 662, 1406, 3295
AcsI RAATTY 6 cut(s) 346, 621, 870, 1846, 2958, 3001
AcuI CTGAAG 2 cut(s) 860, 3579
AcyI GRCGYC 3 cut(s) 255, 378, 402
AfaI GTAC 9 cut(s) 8, 182, 1073, 1129, 1677, 1703, 2678, 3342, 3567
AfeI AGCGCT 1 cut(s) 3246
AfiI CCNNNNNNNGG 7 cut(s) 22, 411, 1883, 1970, 2314, 2450, 3423
AflII CTTAAG 1 cut(s) 3629
AflIII ACRYGT 1 cut(s) 1324
AhdI GACNNNNNGTC 1 cut(s) 3616
AhlI ACTAGT 1 cut(s) 2012
AjnI CCWGG 8 cut(s) 21, 264, 372, 518, 1804, 2111, 2167, 3071
AjuI GAANNNNNNNTTGG 2 cut(s) 3194, 3226
Alw21I GWGCWC 3 cut(s) 1480, 1522, 3048
Alw26I GTCTC 7 cut(s) 97, 279, 423, 1033, 2003, 2060, 3265
AlwI GGATC 8 cut(s) 1480, 1866, 1883, 2486, 2499, 2525, 2568, 3276
AlwNI CAGNNNCTG 2 cut(s) 2666, 3557
Ama87I CYCGRG 3 cut(s) 276, 1496, 2929
Aor51HI AGCGCT 1 cut(s) 3246
AoxI GGCC 7 cut(s) 140, 662, 1406, 1802, 2316, 2515, 3295
ApoI RAATTY 6 cut(s) 346, 621, 870, 1846, 2958, 3001
ArsI GACNNNNNNTTYG 2 cut(s) 2263, 2295
Asp700I GAANNNNTTC 3 cut(s) 1368, 2296, 2738
Asp718I GGTACC 2 cut(s) 180, 3340
AspLEI GCGC 2 cut(s) 426, 3247
AspS9I GGNCC 4 cut(s) 39, 105, 1900, 3660
AsuC2I CCSGG 6 cut(s) 1252, 1497, 1498, 2853, 3017, 3424
AsuHPI GGTGA 5 cut(s) 2183, 2411, 3004, 3389, 3406
AsuNHI GCTAGC 1 cut(s) 1909
AvaI CYCGRG 3 cut(s) 276, 1496, 2929
AvaII GGWCC 4 cut(s) 39, 105, 1900, 3660
BaeGI GKGCMC 2 cut(s) 3093, 3315
BaeI ACNNNNGTAYC 2 cut(s) 3332, 3365
BamHI GGATCC 1 cut(s) 2491
BanI GGYRCC 5 cut(s) 180, 328, 3304, 3340, 3356
BanII GRGCYC 1 cut(s) 840
BauI CACGAG 2 cut(s) 2595, 3229
BbsI GAAGAC 1 cut(s) 2971
Bbv12I GWGCWC 3 cut(s) 1480, 1522, 3048
BbvCI CCTCAGC 2 cut(s) 314, 627
BceAI ACGGC 4 cut(s) 127, 316, 573, 1817
BcgI CGANNNNNNTGC 2 cut(s) 2597, 2631
BciT130I CCWGG 8 cut(s) 23, 266, 374, 520, 1806, 2113, 2169, 3073
BciVI GTATCC 1 cut(s) 3104
BclI TGATCA 1 cut(s) 547
BcnI CCSGG 6 cut(s) 1252, 1497, 1498, 2853, 3017, 3424
BcoDI GTCTC 7 cut(s) 97, 279, 423, 1033, 2003, 2060, 3265
BcuI ACTAGT 1 cut(s) 2012
BfaI CTAG 9 cut(s) 600, 1137, 1541, 1662, 1893, 1910, 1932, 1991, 2013
BfmI CTRYAG 2 cut(s) 1114, 1176
BfoI RGCGCY 2 cut(s) 427, 3248
BfrI CTTAAG 1 cut(s) 3629
BfuI GTATCC 1 cut(s) 3104
BglII AGATCT 1 cut(s) 2398
BlpI GCTNAGC 1 cut(s) 1449
BmcAI AGTACT 1 cut(s) 2678
Bme18I GGWCC 4 cut(s) 39, 105, 1900, 3660
BmeRI GACNNNNNGTC 1 cut(s) 3616
BmeT110I CYCGRG 3 cut(s) 276, 1496, 2929
BmgT120I GGNCC 4 cut(s) 39, 105, 1900, 3660
BmrI ACTGGG 2 cut(s) 1505, 1560
BmtI GCTAGC 1 cut(s) 1913
BmuI ACTGGG 2 cut(s) 1505, 1560
BoxI GACNNNNGTC 1 cut(s) 2507
BpiI GAAGAC 1 cut(s) 2971
BplI GAGNNNNNCTC 2 cut(s) 1442, 1474
BpmI CTGGAG 3 cut(s) 912, 1550, 2704
Bpu10I CCTNAGC 2 cut(s) 314, 627
Bpu1102I GCTNAGC 1 cut(s) 1449
BpuEI CTTGAG 2 cut(s) 1611, 2738
BpuMI CCSGG 6 cut(s) 1252, 1497, 1498, 2853, 3017, 3424
BsaAI YACGTR 1 cut(s) 127
BsaBI GATNNNNATC 3 cut(s) 546, 714, 2814
BsaHI GRCGYC 3 cut(s) 255, 378, 402
BsaI GGTCTC 1 cut(s) 3265
BsaXI ACNNNNNCTCC 2 cut(s) 1712, 1742
Bsc4I CCNNNNNNNGG 7 cut(s) 22, 411, 1883, 1970, 2314, 2450, 3423
Bse118I RCCGGY 2 cut(s) 177, 1404
Bse1I ACTGG 9 cut(s) 567, 1045, 1511, 1555, 2495, 3031, 3261, 3308, 3469
Bse3DI GCAATG 1 cut(s) 3407
Bse8I GATNNNNATC 3 cut(s) 546, 714, 2814
BseBI CCWGG 8 cut(s) 23, 266, 374, 520, 1806, 2113, 2169, 3073
BseGI GGATG 9 cut(s) 164, 579, 1239, 1678, 1843, 1873, 2108, 2764, 3091
BseJI GATNNNNATC 3 cut(s) 546, 714, 2814
BseLI CCNNNNNNNGG 7 cut(s) 22, 411, 1883, 1970, 2314, 2450, 3423
BseMI GCAATG 1 cut(s) 3407
BseNI ACTGG 9 cut(s) 567, 1045, 1511, 1555, 2495, 3031, 3261, 3308, 3469
BseRI GAGGAG 4 cut(s) 331, 407, 1300, 1450
BseSI GKGCMC 2 cut(s) 3093, 3315
BseYI CCCAGC 3 cut(s) 154, 1131, 3103
Bsh1236I CGCG 8 cut(s) 102, 241, 260, 326, 386, 540, 1104, 1353
BshFI GGCC 7 cut(s) 142, 664, 1408, 1804, 2318, 2517, 3297
BshNI GGYRCC 5 cut(s) 180, 328, 3304, 3340, 3356
BsiHKAI GWGCWC 3 cut(s) 1480, 1522, 3048
BsiHKCI CYCGRG 3 cut(s) 276, 1496, 2929
BsiSI CCGG 7 cut(s) 178, 1252, 1405, 1497, 2852, 3016, 3423
BslFI GGGAC 4 cut(s) 37, 118, 266, 1259
BslI CCNNNNNNNGG 7 cut(s) 22, 411, 1883, 1970, 2314, 2450, 3423
BsmAI GTCTC 7 cut(s) 97, 279, 423, 1033, 2003, 2060, 3265
BsmBI CGTCTC 3 cut(s) 97, 279, 423
BsmFI GGGAC 4 cut(s) 37, 118, 266, 1259
BsmI GAATGC 1 cut(s) 226
BsnI GGCC 7 cut(s) 142, 664, 1408, 1804, 2318, 2517, 3297
Bso31I GGTCTC 1 cut(s) 3265
BsoBI CYCGRG 3 cut(s) 276, 1496, 2929
Bsp1286I GDGCHC 6 cut(s) 840, 1480, 1522, 3048, 3093, 3315
Bsp1720I GCTNAGC 1 cut(s) 1449
Bsp19I CCATGG 3 cut(s) 861, 1152, 2969
BspANI GGCC 7 cut(s) 142, 664, 1408, 1804, 2318, 2517, 3297
BspFNI CGCG 8 cut(s) 102, 241, 260, 326, 386, 540, 1104, 1353
BspHI TCATGA 3 cut(s) 1345, 1594, 3126
BspMAI CTGCAG 2 cut(s) 1118, 1180
BspOI GCTAGC 1 cut(s) 1913
BspPI GGATC 8 cut(s) 1480, 1866, 1883, 2486, 2499, 2525, 2568, 3276
BspQI GCTCTTC 1 cut(s) 1640
BspT107I GGYRCC 5 cut(s) 180, 328, 3304, 3340, 3356
BspTI CTTAAG 1 cut(s) 3629
BspTNI GGTCTC 1 cut(s) 3265
BsrDI GCAATG 1 cut(s) 3407
BsrFI RCCGGY 2 cut(s) 177, 1404
BsrI ACTGG 9 cut(s) 567, 1045, 1511, 1555, 2495, 3031, 3261, 3308, 3469
BssAI RCCGGY 2 cut(s) 177, 1404
BssNI GRCGYC 3 cut(s) 255, 378, 402
BssSI CACGAG 2 cut(s) 2595, 3229
BssT1I CCWWGG 5 cut(s) 751, 861, 1152, 1709, 2969
Bst2BI CACGAG 2 cut(s) 2595, 3229
Bst2UI CCWGG 8 cut(s) 23, 266, 374, 520, 1806, 2113, 2169, 3073
Bst4CI ACNGT 9 cut(s) 85, 791, 993, 1706, 2407, 2486, 2506, 2586, 3484
Bst6I CTCTTC 2 cut(s) 1640, 2733
BstACI GRCGYC 3 cut(s) 255, 378, 402
BstAFI CTTAAG 1 cut(s) 3629
BstAPI GCANNNNNTGC 1 cut(s) 3519
BstBAI YACGTR 1 cut(s) 127
BstC8I GCNNGC 7 cut(s) 226, 262, 890, 969, 1406, 1911, 2515
BstDSI CCRYGG 6 cut(s) 100, 324, 861, 1152, 1269, 2969
BstENI CCTNNNNNAGG 2 cut(s) 20, 2312
BstF5I GGATG 9 cut(s) 164, 579, 1239, 1678, 1843, 1873, 2108, 2764, 3091
BstFNI CGCG 8 cut(s) 102, 241, 260, 326, 386, 540, 1104, 1353
BstH2I RGCGCY 2 cut(s) 427, 3248
BstHHI GCGC 2 cut(s) 426, 3247
BstMAI GTCTC 7 cut(s) 97, 279, 423, 1033, 2003, 2060, 3265
BstMWI GCNNNNNNNGC 8 cut(s) 148, 266, 307, 835, 904, 2573, 3519, 3560
BstNI CCWGG 8 cut(s) 23, 266, 374, 520, 1806, 2113, 2169, 3073
BstNSI RCATGY 2 cut(s) 1328, 1917
BstPAI GACNNNNGTC 1 cut(s) 2507
BstSFI CTRYAG 2 cut(s) 1114, 1176
BstSLI GKGCMC 2 cut(s) 3093, 3315
BstUI CGCG 8 cut(s) 102, 241, 260, 326, 386, 540, 1104, 1353
BstV2I GAAGAC 1 cut(s) 2971
BstX2I RGATCY 5 cut(s) 1888, 2398, 2491, 2530, 2560
BstXI CCANNNNNNTGG 2 cut(s) 434, 2970
BstYI RGATCY 5 cut(s) 1888, 2398, 2491, 2530, 2560
BsuI GTATCC 1 cut(s) 3104
BsuRI GGCC 7 cut(s) 142, 664, 1408, 1804, 2318, 2517, 3297
BtgI CCRYGG 6 cut(s) 100, 324, 861, 1152, 1269, 2969
BtsCI GGATG 9 cut(s) 164, 579, 1239, 1678, 1843, 1873, 2108, 2764, 3091
BtsI GCAGTG 1 cut(s) 193
BtsIMutI CAGTG 7 cut(s) 193, 560, 989, 2412, 2433, 3315, 3489
Cac8I GCNNGC 7 cut(s) 226, 262, 890, 969, 1406, 1911, 2515
CaiI CAGNNNCTG 2 cut(s) 2666, 3557
CciI TCATGA 3 cut(s) 1345, 1594, 3126
CfoI GCGC 2 cut(s) 426, 3247
Cfr10I RCCGGY 2 cut(s) 177, 1404
Cfr13I GGNCC 4 cut(s) 39, 105, 1900, 3660
Cfr42I CCGCGG 2 cut(s) 103, 327
Cfr9I CCCGGG 1 cut(s) 1496
CseI GACGC 5 cut(s) 230, 386, 410, 546, 3557
CsiI ACCWGGT 1 cut(s) 2167
Csp6I GTAC 9 cut(s) 7, 181, 1072, 1128, 1676, 1702, 2677, 3341, 3566
CspCI CAANNNNNGTGG 2 cut(s) 3589, 3624
CviQI GTAC 9 cut(s) 7, 181, 1072, 1128, 1676, 1702, 2677, 3341, 3566
DrdI GACNNNNNNGTC 1 cut(s) 1720
DriI GACNNNNNGTC 1 cut(s) 3616
DseDI GACNNNNNNGTC 1 cut(s) 1720
EaeI YGGCCR 3 cut(s) 662, 1406, 3295
Eam1104I CTCTTC 2 cut(s) 1640, 2733
Eam1105I GACNNNNNGTC 1 cut(s) 3616
EarI CTCTTC 2 cut(s) 1640, 2733
EciI GGCGGA 2 cut(s) 195, 299
Eco130I CCWWGG 5 cut(s) 751, 861, 1152, 1709, 2969
Eco147I AGGCCT 2 cut(s) 2318, 2517
Eco24I GRGCYC 1 cut(s) 840
Eco31I GGTCTC 1 cut(s) 3265
Eco32I GATATC 1 cut(s) 1146
Eco47I GGWCC 4 cut(s) 39, 105, 1900, 3660
Eco47III AGCGCT 1 cut(s) 3246
Eco57I CTGAAG 2 cut(s) 860, 3579
Eco88I CYCGRG 3 cut(s) 276, 1496, 2929
EcoNI CCTNNNNNAGG 2 cut(s) 20, 2312
EcoO109I RGGNCCY 3 cut(s) 105, 1900, 3660
EcoRI GAATTC 1 cut(s) 3001
EcoRII CCWGG 8 cut(s) 21, 264, 372, 518, 1804, 2111, 2167, 3071
EcoRV GATATC 1 cut(s) 1146
EcoT14I CCWWGG 5 cut(s) 751, 861, 1152, 1709, 2969
EcoT38I GRGCYC 1 cut(s) 840
ErhI CCWWGG 5 cut(s) 751, 861, 1152, 1709, 2969
Esp3I CGTCTC 3 cut(s) 97, 279, 423
FalI AAGNNNNNCTT 6 cut(s) 1209, 1241, 1356, 1388, 2726, 2758
FaqI GGGAC 4 cut(s) 37, 118, 266, 1259
FauI CCCGC 4 cut(s) 95, 1236, 2755, 3142
FauNDI CATATG 2 cut(s) 2074, 3615
FbaI TGATCA 1 cut(s) 547
FblI GTMKAC 1 cut(s) 2281
FokI GGATG 9 cut(s) 171, 586, 1246, 1685, 1850, 1860, 2095, 2771, 3098
FriOI GRGCYC 1 cut(s) 840
FspBI CTAG 9 cut(s) 600, 1137, 1541, 1662, 1893, 1910, 1932, 1991, 2013
GlaI GCGC 2 cut(s) 425, 3246
GsaI CCCAGC 3 cut(s) 158, 1135, 3107
GsuI CTGGAG 3 cut(s) 912, 1550, 2704
HaeII RGCGCY 2 cut(s) 427, 3248
HaeIII GGCC 7 cut(s) 142, 664, 1408, 1804, 2318, 2517, 3297
HapII CCGG 7 cut(s) 178, 1252, 1405, 1497, 2852, 3016, 3423
HgaI GACGC 5 cut(s) 230, 386, 410, 546, 3557
HhaI GCGC 2 cut(s) 426, 3247
Hin1I GRCGYC 3 cut(s) 255, 378, 402
Hin6I GCGC 2 cut(s) 424, 3245
HinP1I GCGC 2 cut(s) 424, 3245
HincII GTYRAC 3 cut(s) 477, 1300, 2773
HindII GTYRAC 3 cut(s) 477, 1300, 2773
HindIII AAGCTT 1 cut(s) 886
HpaI GTTAAC 1 cut(s) 477
HpaII CCGG 7 cut(s) 178, 1252, 1405, 1497, 2852, 3016, 3423
HphI GGTGA 5 cut(s) 2183, 2411, 3004, 3389, 3406
Hpy166II GTNNAC 8 cut(s) 477, 1300, 1503, 2267, 2282, 2773, 2872, 3600
Hpy8I GTNNAC 8 cut(s) 477, 1300, 1503, 2267, 2282, 2773, 2872, 3600
Hpy99I CGWCG 3 cut(s) 148, 260, 404
HpyAV CCTTC 7 cut(s) 926, 1223, 1849, 2551, 3316, 3331, 3538
HpyCH4III ACNGT 9 cut(s) 85, 791, 993, 1706, 2407, 2486, 2506, 2586, 3484
HpyCH4IV ACGT 2 cut(s) 126, 255
HpyF10VI GCNNNNNNNGC 8 cut(s) 148, 266, 307, 835, 904, 2573, 3519, 3560
HpySE526I ACGT 2 cut(s) 126, 255
Hsp92I GRCGYC 3 cut(s) 255, 378, 402
HspAI GCGC 2 cut(s) 424, 3245
KflI GGGWCCC 1 cut(s) 105
KpnI GGTACC 2 cut(s) 184, 3344
KroI GCCGGC 1 cut(s) 1404
KroNI GCCGGC 1 cut(s) 1406
Ksp22I TGATCA 1 cut(s) 547
KspAI GTTAAC 1 cut(s) 477
KspI CCGCGG 2 cut(s) 103, 327
LguI GCTCTTC 1 cut(s) 1640
MabI ACCWGGT 1 cut(s) 2167
MaeI CTAG 9 cut(s) 600, 1137, 1541, 1662, 1893, 1910, 1932, 1991, 2013
MaeII ACGT 2 cut(s) 126, 255
MaeIII GTNAC 3 cut(s) 1211, 1318, 2177
MflI RGATCY 5 cut(s) 1888, 2398, 2491, 2530, 2560
MhlI GDGCHC 6 cut(s) 840, 1480, 1522, 3048, 3093, 3315
MlyI GAGTC 8 cut(s) 404, 1708, 1730, 1739, 2239, 2579, 2672, 2717
MmeI TCCRAC 2 cut(s) 423, 2621
MroNI GCCGGC 1 cut(s) 1404
MroXI GAANNNNTTC 3 cut(s) 1368, 2296, 2738
MseI TTAA 8 cut(s) 476, 995, 1758, 3161, 3350, 3575, 3630, 3687
MslI CAYNNNNRTG 2 cut(s) 47, 2968
MspA1I CMGCKG 6 cut(s) 102, 211, 313, 326, 880, 3554
MspCI CTTAAG 1 cut(s) 3629
MspI CCGG 7 cut(s) 178, 1252, 1405, 1497, 2852, 3016, 3423
Mva1269I GAATGC 1 cut(s) 226
MvaI CCWGG 8 cut(s) 23, 266, 374, 520, 1806, 2113, 2169, 3073
MvnI CGCG 8 cut(s) 102, 241, 260, 326, 386, 540, 1104, 1353
MwoI GCNNNNNNNGC 8 cut(s) 148, 266, 307, 835, 904, 2573, 3519, 3560
NaeI GCCGGC 1 cut(s) 1406
NciI CCSGG 6 cut(s) 1252, 1497, 1498, 2853, 3017, 3424
NcoI CCATGG 3 cut(s) 861, 1152, 2969
NdeI CATATG 2 cut(s) 2074, 3615
NgoMIV GCCGGC 1 cut(s) 1404
NheI GCTAGC 1 cut(s) 1909
NmeAIII GCCGAG 1 cut(s) 2113
NspI RCATGY 2 cut(s) 1328, 1917
PaeR7I CTCGAG 1 cut(s) 2929
PagI TCATGA 3 cut(s) 1345, 1594, 3126
PceI AGGCCT 2 cut(s) 2318, 2517
PciI ACATGT 1 cut(s) 1324
PciSI GCTCTTC 1 cut(s) 1640
PctI GAATGC 1 cut(s) 226
PdiI GCCGGC 1 cut(s) 1406
PdmI GAANNNNTTC 3 cut(s) 1368, 2296, 2738
PflMI CCANNNNNTGG 1 cut(s) 1970
PfoI TCCNGGA 3 cut(s) 2111, 2851, 3015
PleI GAGTC 8 cut(s) 403, 1708, 1729, 1739, 2239, 2578, 2671, 2717
PpsI GAGTC 8 cut(s) 403, 1708, 1729, 1739, 2239, 2578, 2671, 2717
Ppu21I YACGTR 1 cut(s) 127
PpuMI RGGWCCY 3 cut(s) 105, 1900, 3660
PscI ACATGT 1 cut(s) 1324
PshAI GACNNNNGTC 1 cut(s) 2507
Psp5II RGGWCCY 3 cut(s) 105, 1900, 3660
Psp6I CCWGG 8 cut(s) 21, 264, 372, 518, 1804, 2111, 2167, 3071
PspFI CCCAGC 3 cut(s) 154, 1131, 3103
PspGI CCWGG 8 cut(s) 21, 264, 372, 518, 1804, 2111, 2167, 3071
PspPI GGNCC 4 cut(s) 39, 105, 1900, 3660
PspPPI RGGWCCY 3 cut(s) 105, 1900, 3660
PspXI VCTCGAGB 1 cut(s) 2929
PsrI GAACNNNNNNTAC 4 cut(s) 164, 196, 3371, 3403
PstI CTGCAG 2 cut(s) 1118, 1180
PstNI CAGNNNCTG 2 cut(s) 2666, 3557
PsuI RGATCY 5 cut(s) 1888, 2398, 2491, 2530, 2560
PvuII CAGCTG 2 cut(s) 313, 3554
RsaI GTAC 9 cut(s) 8, 182, 1073, 1129, 1677, 1703, 2678, 3342, 3567
RsaNI GTAC 9 cut(s) 7, 181, 1072, 1128, 1676, 1702, 2677, 3341, 3566
RseI CAYNNNNRTG 2 cut(s) 47, 2968
SacII CCGCGG 2 cut(s) 103, 327
SapI GCTCTTC 1 cut(s) 1640
SaqAI TTAA 8 cut(s) 476, 995, 1758, 3161, 3350, 3575, 3630, 3687
Sau96I GGNCC 4 cut(s) 39, 105, 1900, 3660
ScaI AGTACT 1 cut(s) 2678
SchI GAGTC 8 cut(s) 404, 1708, 1730, 1739, 2239, 2579, 2672, 2717
SduI GDGCHC 6 cut(s) 840, 1480, 1522, 3048, 3093, 3315
SexAI ACCWGGT 1 cut(s) 2167
SfcI CTRYAG 2 cut(s) 1114, 1176
Sfr274I CTCGAG 1 cut(s) 2929
Sfr303I CCGCGG 2 cut(s) 103, 327
SgrBI CCGCGG 2 cut(s) 103, 327
SinI GGWCC 4 cut(s) 39, 105, 1900, 3660
SlaI CTCGAG 1 cut(s) 2929
SmaI CCCGGG 1 cut(s) 1498
SmiMI CAYNNNNRTG 2 cut(s) 47, 2968
SmlI CTYRAG 4 cut(s) 1626, 2753, 2929, 3629
SmoI CTYRAG 4 cut(s) 1626, 2753, 2929, 3629
SpeI ACTAGT 1 cut(s) 2012
SseBI AGGCCT 2 cut(s) 2318, 2517
SspI AATATT 1 cut(s) 1537
SspMI CTAG 9 cut(s) 600, 1137, 1541, 1662, 1893, 1910, 1932, 1991, 2013
StuI AGGCCT 2 cut(s) 2318, 2517
StyI CCWWGG 5 cut(s) 751, 861, 1152, 1709, 2969
TaaI ACNGT 9 cut(s) 85, 791, 993, 1706, 2407, 2486, 2506, 2586, 3484
TaiI ACGT 2 cut(s) 129, 258
TaqI TCGA 5 cut(s) 146, 440, 2930, 3458, 3620
TatI WGTACW 5 cut(s) 1071, 1675, 1701, 2676, 3565
TauI GCSGC 7 cut(s) 120, 211, 241, 284, 664, 2548, 3300
Tru1I TTAA 8 cut(s) 476, 995, 1758, 3161, 3350, 3575, 3630, 3687
Tru9I TTAA 8 cut(s) 476, 995, 1758, 3161, 3350, 3575, 3630, 3687
TscAI CASTG 7 cut(s) 200, 567, 996, 2412, 2433, 3315, 3489
TspGWI ACGGA 2 cut(s) 1156, 3464
TspMI CCCGGG 1 cut(s) 1496
TspRI CASTG 7 cut(s) 200, 567, 996, 2412, 2433, 3315, 3489
Van91I CCANNNNNTGG 1 cut(s) 1970
Vha464I CTTAAG 1 cut(s) 3629
VpaK11BI GGWCC 4 cut(s) 39, 105, 1900, 3660
XagI CCTNNNNNAGG 2 cut(s) 20, 2312
XapI RAATTY 6 cut(s) 346, 621, 870, 1846, 2958, 3001
XbaI TCTAGA 2 cut(s) 599, 1540
XceI RCATGY 2 cut(s) 1328, 1917
XcmI CCANNNNNNNNNTGG 1 cut(s) 3079
XhoI CTCGAG 1 cut(s) 2929
XmaI CCCGGG 1 cut(s) 1496
XmiI GTMKAC 1 cut(s) 2281
XmnI GAANNNNTTC 3 cut(s) 1368, 2296, 2738
XspI CTAG 9 cut(s) 600, 1137, 1541, 1662, 1893, 1910, 1932, 1991, 2013
ZraI GACGTC 1 cut(s) 256
ZrmI AGTACT 1 cut(s) 2678
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.