RLG00000005374

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0013882
Physical Location & Seq
Forward (+)
67176532 .. 67183654
7123 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005374

Sequence Viewer

Length: 3681 bp
ATGCCTTACTCCGCCTTCTTTCACCGCTCTATAATAATCCGCACCACCTCCACCGCCATGTCCCTCAAACCCCGCCACCACCACTTCTCTCTCCTCCGGCCGCCGTCGCTCCTCCTCGGATGCTTCATGAACACCAACGGCCACTACCACACTCACCCCGTCACCGGCCTGCTCAAATGGAATTCCGCCGGCTGCTGCCGTCACCCTGACGTCACGTGCCTGGCTTCCTCCTCCTCCTCGTCTTCGCGTGCGGCGGAGAGGACGGATTGGAAGCAGCGGCGGAGGAGCGTTGCGGCCGTGCCGGCGTGGTATCAGCAGCAGCAGAAGCTGGGGTACGGGAGGTTTGCGTACCAGGACGAGTCGAGTAGTGAAGAATCGGACCGGGAGTTCGGGTCGGGTCAGAGTTCTCGCCGGGAAATGACTGGTTCGACCCTTGACAACATCGATGAGTGGAGATGGAAGTTAACCATGCTTGTTCGCAACAAAGATGAGCAAGAAGTGGTGTCGAGGGAAAGGAAGGACAGGCGTGATTTTGATCATCTTTCAGAACTGGCAAGAGGGATGGGTTTATATAGCCGTCAGTATTCAAAAGTTGTAGTCTTTAGTAAAGTCCCGCAGCCAAATTACAGACCTGATCTGGATGATAGGCGACCACAAAGAGAGGTTGTCTTACCTTTTGGACTGCATAAAGGTGTAGATGCTCATCTCAAAGCTCATCTTTCTCAAAAGCCAATGAGCAGAGGAAATTTATCACATACTTCAATGTCAAGGTCAAATAGTAATGGAAGTATAGCTAAAGATGGGGGACTCTACGAGCAGGAGGAGCCCTTGATACAGAATAGTGTTGCCATGGAGAGAATTCTTCAGCAGAAAAGCTTGCGATTGCGCAATAAGCAACAAGAATGGCAGGAATCCATCGAAGGTCAAAAGATGCTTGAACTTCGTAGAAGTCTGCCTGCATATAAAGAGAAAGATTTCTTGTTAAAAGCCATATCCGAAAATCAGGTCATAGTTGTTTCAGGTGAAACAGGTTGTGGTAAAACTACACAACTTCCTCAGTACATTTTAGAATCTGAGATTGAAGCTGGTCGTGGAGGTGTCTGTAGTATTATTTGTACTCAGCCTAGACGAATATCTGCCATGTCTGTTTCTGAAAGAGTTGCTGCAGAACGAGGGGAGAATCTGGGGGAATCAGTTGGTTACAAAGTTAGGCTGGAAGGAATGAAAGGGAGGGACACGCGGCTTCTTTTTTGCACCACAGGCATACTATTGAGAAGATTACTTGTCGACAGAAAGTTGAGAGGTGTTACACATGTCATTGTTGATGAGATTCATGAACGCGGAATGAATGAAGATTTTCTTCTTATCGTTCTGAAAGAACTTCTCTCTCGTCGTCCTGAGTTGAGATTGATTTTGATGAGTGCAACCTTAAATGCTGAGCTTTTCTCCTTGTACTTTAATGGTGCTCCAATGATCCATATACCTGGTTTTACATACCCAGTCCGAGCACATTTTCTGGAGAATATTCTAGAAATGACTGGATATCGGTTAAATCAATATAATCAAATTGATGATTATGGTCAAGATAAGACGTGGAAAATGCAGAAACAAGCTCAAGCTTTCAAAAAGAGGAAGAGCCAAATTGCTTCTACTGTTGAGGATGCACTTGAAGCTGCTGACTTTAGGGAGTACAGTCCAAGGACTCAGGAGTCTCTGTCCTGTTGGAATCCGGACTCCATTGGTTTTAATCTAATTGAGCATGTGCTTTGCCACATAGTCAGGAAAGAACGGCCTGGTGCTATTTTGGTTTTTATGACTGGTTGGGATGACATAAACTCCTTGAAGGATCAACTCCAATCTCATCCCCTTTTAGGAGATCCTAGTAGGGTCTTGCTGCTTGCCTGTCATGGTTCTATGCCTAGCTCTGAACAGAGGTTGATATTTGATAAACCAGAAGATGGAGTTAGGAAAATCGTTCTGGCTACAAACATGGCTGAGACCAGTATCACTATCAATGATGTAGTATTCGTGATTGATTGTGGAAAGGCGAAAGAAACATCATATGATGCACTCAATAATACTCCCTGTTTGCTTCCATCCTGGATATCAAAGGCTGCTTCCCGGCAAAGAAGAGGAAGAGCCGGTCGTGTTCAACCTGGCGAGTGTTACCATCTATATCCCCGATGTGTATATGATGCCTTTGCTGATTATCAATTGCCTGAACTTTTGAGGACGCCTCTGCAGTCTCTATGTTTACAAATCAAGAGTCTACAACTTGGAAGTATTTCTGAGTTCCTTTCTAAGGCACTGCAGTCACCGGAACCTTTGTCGGTTCAAAATGCTGTTGATTATCTGAAGATCATAGGGGCTTTAGATGATAATGAAGATCTCACAGTATTAGGTCGCCACCTGTCAATGCTTCCGGTTGAGCCAAAACTTGGGAAAATGCTCATATTAGGGGCTATCTTCAACTGTTTAGATCCAATAATGACTATTGTTGCAGGCCTTAGTATGAGAGATCCATTCATGATGCCATTTGATAAGAAGGATCTTGCGGAATCGGCAAAAGCACAATTTTCTGGCCGTGATAGCAGTGATCATCTGGCTCTTATCCGAGCATATGATGGTTGGAAAAATGCTGAAAGATCGCAAGCTGGTTATGAGTACTGCTGGAGAAATTTTCTTTCTGCACAAACTCTAAAAGCCATCGACTCTCTTCGGAAGCAGTTCTTCTTCTTGCTCAAGGATGCTGGTTTGGTTGATCACAACATCGAGACCTGCAATACATTGAGCCATGATGAACATCTTATCCGAGCAATCATCTGTGCAGGATTATTTCCTGGAATATGTTCTGTTGTGAACAAAGAGAAGTCGATTTCATTGAAAACAATGGAAGATGGACAAGTACTTCTATACTCGAATTCTGTCAATGCTTCGGTGCCTAAAATTCCATATCCATGGCTAGTTTTCAACGAAAAGGTGAAAGTGAATTCAGTATTCCTTCGGGATTCAACTGGCGTATCTGATTCTGTGCTCCTTTTGTTTGGAGGCAACATTTCCAGGGGTGGACTGGATGGACACCTGAAGATGTTGGGAGGATACTTGGAGTTCTTCATGAATCCTGCATTAGCAAATACATATGTTAGCTTAAAGAGGGAACTTGAGGAACTGATTCATAATAAGCTTATAGATCCCAAATTGGATATGCAATCTCATACTAACCTCCTATCAGCCCTGAGATTACTGGTCTCTGAAGACCGATGTGATGGTAGATTTGTCTTTGGTCGCAAGATGCCAGTACCCTCAAAGAAGATAACAAAAGAGTTAGGTCCAGGCACCTTGAGGGTTGGCGATAATGGTAAAAGCGGGGGTAACAATTCCAAAAGTCAGCTGCAAACATTGCTTGTCAGAGCAGGACATGAAGCTCCCACCTACAAAACAAAACAACTGAAAAACAACCAGTTTCGGTCCACAGTGATCTTCAATGGGTTGAACTTTGTAGGGCAGCCTTCCAATAGTAAGAAACAAGCAGAGAAGGAAGCGGCTGCTGAAGCTGTGCTCTGGTTAAAGGGCGATAACCATTCATCTTCCACAGATATTGACCATATGTCAATGCTTCTAAAGAAGAGTAGAAAGAAAATCCATAAAACGACCTCATTTGATAGCGCTAAGTGGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001047 GO:0001067 GO:0001503 GO:0001817 GO:0001819 GO:0002151 GO:0002791 GO:0002793 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003697 GO:0003723 GO:0003724 GO:0003725 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006355 GO:0006357 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0008186 GO:0009605 GO:0009607 GO:0009615 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010501 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010638 GO:0014070 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0019899 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031330 GO:0032204 GO:0032206 GO:0032479 GO:0032481 GO:0032501 GO:0032647 GO:0032727 GO:0032879 GO:0032880 GO:0033043 GO:0033044 GO:0034641 GO:0042221 GO:0042623 GO:0042826 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043330 GO:0043331 GO:0043487 GO:0043489 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050707 GO:0050708 GO:0050714 GO:0050715 GO:0050789 GO:0050794 GO:0050896 GO:0051046 GO:0051047 GO:0051049 GO:0051050 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051222 GO:0051223 GO:0051239 GO:0051240 GO:0051252 GO:0051253 GO:0051254 GO:0051704 GO:0051707 GO:0051880 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070035 GO:0070201 GO:0071704 GO:0080090 GO:0090087 GO:0090304 GO:0090669 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1902369 GO:1902680 GO:1902739 GO:1902741 GO:1903506 GO:1903508 GO:1903530 GO:1903532 GO:1904951 GO:1990837 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind dsrm

Protein Analysis

1227

Amino Acids

138.32

Weight (kDa)

9.08

Isoelectric Point (pI)

50.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 329 - 480 3.3e-10 DEAD/DEAH box helicase
Helicase_C PF00271 588 - 717 9.1e-13 Helicase conserved C-terminal domain
WHD_HA2 PF04408 781 - 809 3.3e-08 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 810 - 870 1.8e-14 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 937 - 1019 9.9e-20 Oligonucleotide/oligosaccharide-binding (OB)-fold
RNA_hel_CTD PF26026 1027 - 1085 2.1e-18 C-terminal domain in RNA helicases
dsrm PF00035 1128 - 1189 1.1e-08 Double-stranded RNA binding motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013882)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G48650 AT1G48650
fragaria_vesca FvH4_5g16860
malus_domestica MD06G1222500.v1.1 MD14G1231600.v1.1
prunus_persica Prupe.5G227100_v2.0.a1
pyrus_communis pycom06g19810 pycom06g19820 pycom14g19280
rosa_chinensis RchiOBHm_Chr7g0179211
rosa_laevigata RLG00000005374
rosa_multiflora Rmu_sc0009798.1_g000005 Rmu_sc0021799.1_g000001
rosa_roxburghii Rroxscaffold_3G00274220
rosa_rugosa Rorug06G0422200
rosa_samantha Rh7BG021500
rosa_wichuraiana Rw7G001770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1708
AatII GACGTC 1 cut(s) 213
Acc16I TGCGCA 1 cut(s) 887
Acc36I ACCTGC 1 cut(s) 2786
AccB1I GGYRCC 2 cut(s) 2938, 3335
AccB7I CCANNNNNTGG 2 cut(s) 1958, 2438
AccBSI CCGCTC 1 cut(s) 27
AccI GTMKAC 2 cut(s) 1287, 2269
AccII CGCG 3 cut(s) 247, 1240, 1341
AccIII TCCGGA 1 cut(s) 1729
AclWI GGATC 7 cut(s) 1468, 1854, 1871, 2474, 2513, 2556, 3185
AcoI YGGCCR 4 cut(s) 98, 139, 294, 2581
AcsI RAATTY 7 cut(s) 181, 745, 858, 2677, 2920, 2946, 2989
AcuI CTGAAG 5 cut(s) 848, 2375, 3104, 3273, 3570
AcvI CACGTG 1 cut(s) 216
AcyI GRCGYC 2 cut(s) 210, 2234
AfaI GTAC 9 cut(s) 335, 350, 1061, 1117, 1454, 1691, 2666, 2907, 3300
AfeI AGCGCT 1 cut(s) 3667
AfiI CCNNNNNNNGG 5 cut(s) 164, 1871, 1958, 2302, 2438
AflIII ACRYGT 1 cut(s) 1312
AhdI GACNNNNNGTC 1 cut(s) 3607
AjiI CACGTC 1 cut(s) 1593
AjnI CCWGG 9 cut(s) 219, 351, 1483, 1792, 2099, 2155, 2839, 3059, 3331
AloI GAACNNNNNNTCC 4 cut(s) 371, 403, 2793, 2825
Alw21I GWGCWC 4 cut(s) 1468, 1510, 3036, 3561
Alw26I GTCTC 5 cut(s) 1716, 1991, 2250, 2768, 3253
AlwI GGATC 7 cut(s) 1468, 1854, 1871, 2474, 2513, 2556, 3185
AlwNI CAGNNNCTG 1 cut(s) 328
Aor13HI TCCGGA 1 cut(s) 1729
Aor51HI AGCGCT 1 cut(s) 3667
AoxI GGCC 7 cut(s) 98, 139, 166, 294, 1790, 2503, 2581
ApoI RAATTY 7 cut(s) 181, 745, 858, 2677, 2920, 2946, 2989
ArsI GACNNNNNNTTYG 6 cut(s) 371, 403, 990, 1022, 2251, 2283
Asp700I GAANNNNTTC 6 cut(s) 974, 1356, 2284, 2726, 2848, 3171
AspLEI GCGC 2 cut(s) 888, 3668
AspS9I GGNCC 3 cut(s) 379, 3329, 3468
AsuC2I CCSGG 3 cut(s) 383, 413, 2122
AsuHPI GGTGA 7 cut(s) 14, 146, 154, 194, 1034, 2307, 2992
AvaII GGWCC 3 cut(s) 379, 3329, 3468
BaeI ACNNNNGTAYC 2 cut(s) 3003, 3036
BanI GGYRCC 2 cut(s) 2938, 3335
BanII GRGCYC 1 cut(s) 828
BarI GAAGNNNNNNTAC 2 cut(s) 317, 349
BbrPI CACGTG 1 cut(s) 216
BbsI GAAGAC 2 cut(s) 234, 3261
Bbv12I GWGCWC 4 cut(s) 1468, 1510, 3036, 3561
BceAI ACGGC 7 cut(s) 88, 154, 183, 281, 561, 1805, 2568
BciT130I CCWGG 9 cut(s) 221, 353, 1485, 1794, 2101, 2157, 2841, 3061, 3333
BciVI GTATCC 1 cut(s) 3092
BclI TGATCA 3 cut(s) 535, 2596, 2761
BcnI CCSGG 3 cut(s) 383, 413, 2122
BcoDI GTCTC 5 cut(s) 1716, 1991, 2250, 2768, 3253
BfaI CTAG 5 cut(s) 1125, 1529, 1881, 1920, 2963
BfmI CTRYAG 4 cut(s) 1102, 1164, 2240, 2309
BfoI RGCGCY 1 cut(s) 3669
BfuAI ACCTGC 1 cut(s) 2786
BfuI GTATCC 1 cut(s) 3092
BglI GCCNNNNNGGC 1 cut(s) 302
BglII AGATCT 1 cut(s) 2386
BlpI GCTNAGC 1 cut(s) 1437
BmcAI AGTACT 2 cut(s) 2666, 2907
Bme18I GGWCC 3 cut(s) 379, 3329, 3468
BmeRI GACNNNNNGTC 1 cut(s) 3607
BmgBI CACGTC 1 cut(s) 1593
BmgT120I GGNCC 3 cut(s) 379, 3329, 3468
BmiI GGNNCC 4 cut(s) 825, 2322, 2940, 3337
BmrI ACTGGG 1 cut(s) 1493
BmsI GCATC 9 cut(s) 110, 688, 921, 1651, 2056, 2185, 2520, 2737, 3282
BmuI ACTGGG 1 cut(s) 1493
BpiI GAAGAC 2 cut(s) 234, 3261
BplI GAGNNNNNCTC 4 cut(s) 1430, 1462, 2221, 2253
BpmI CTGGAG 2 cut(s) 1538, 2692
Bpu1102I GCTNAGC 1 cut(s) 1437
BpuEI CTTGAG 4 cut(s) 1599, 2726, 3182, 3361
BpuMI CCSGG 3 cut(s) 383, 413, 2122
Bsa29I ATCGAT 1 cut(s) 444
BsaAI YACGTR 1 cut(s) 216
BsaBI GATNNNNATC 3 cut(s) 534, 702, 2802
BsaHI GRCGYC 2 cut(s) 210, 2234
BsaI GGTCTC 3 cut(s) 1991, 2768, 3253
BsaJI CCNNGG 5 cut(s) 115, 849, 1697, 2957, 3060
BsaWI WCCGGW 3 cut(s) 1729, 2317, 2422
BsaXI ACNNNNNCTCC 6 cut(s) 377, 407, 1700, 1730, 1820, 1850
Bsc4I CCNNNNNNNGG 5 cut(s) 164, 1871, 1958, 2302, 2438
Bse118I RCCGGY 4 cut(s) 164, 188, 301, 2141
Bse3DI GCAATG 1 cut(s) 3398
Bse8I GATNNNNATC 3 cut(s) 534, 702, 2802
BseAI TCCGGA 1 cut(s) 1729
BseBI CCWGG 9 cut(s) 221, 353, 1485, 1794, 2101, 2157, 2841, 3061, 3333
BseCI ATCGAT 1 cut(s) 444
BseDI CCNNGG 5 cut(s) 115, 849, 1697, 2957, 3060
BseGI GGATG 9 cut(s) 125, 567, 646, 1666, 1831, 1861, 2096, 2752, 3079
BseJI GATNNNNATC 3 cut(s) 534, 702, 2802
BseLI CCNNNNNNNGG 5 cut(s) 164, 1871, 1958, 2302, 2438
BseMI GCAATG 1 cut(s) 3398
BseMII CTCAG 9 cut(s) 1065, 1070, 1133, 1389, 1428, 1718, 1986, 2280, 3227
BseRI GAGGAG 8 cut(s) 83, 101, 104, 220, 223, 226, 298, 836
BseX3I CGGCCG 2 cut(s) 98, 294
BseYI CCCAGC 1 cut(s) 328
BsgI GTGCAG 2 cut(s) 2673, 2847
Bsh1236I CGCG 3 cut(s) 247, 1240, 1341
Bsh1285I CGRYCG 3 cut(s) 101, 297, 2146
BshFI GGCC 7 cut(s) 100, 141, 168, 296, 1792, 2505, 2583
BshNI GGYRCC 2 cut(s) 2938, 3335
BshVI ATCGAT 1 cut(s) 444
BsiEI CGRYCG 3 cut(s) 101, 297, 2146
BsiHKAI GWGCWC 4 cut(s) 1468, 1510, 3036, 3561
BslFI GGGAC 4 cut(s) 46, 596, 819, 1247
BslI CCNNNNNNNGG 5 cut(s) 164, 1871, 1958, 2302, 2438
BsmAI GTCTC 5 cut(s) 1716, 1991, 2250, 2768, 3253
BsmFI GGGAC 4 cut(s) 46, 596, 819, 1247
BsnI GGCC 7 cut(s) 100, 141, 168, 296, 1792, 2505, 2583
Bso31I GGTCTC 3 cut(s) 1991, 2768, 3253
Bsp1286I GDGCHC 5 cut(s) 828, 1468, 1510, 3036, 3561
Bsp13I TCCGGA 1 cut(s) 1729
Bsp1720I GCTNAGC 1 cut(s) 1437
Bsp19I CCATGG 2 cut(s) 849, 2957
BspANI GGCC 7 cut(s) 100, 141, 168, 296, 1792, 2505, 2583
BspCNI CTCAG 9 cut(s) 1066, 1069, 1132, 1390, 1429, 1717, 1987, 2281, 3228
BspDI ATCGAT 1 cut(s) 444
BspEI TCCGGA 1 cut(s) 1729
BspFNI CGCG 3 cut(s) 247, 1240, 1341
BspHI TCATGA 4 cut(s) 126, 1333, 2526, 3114
BspLI GGNNCC 4 cut(s) 825, 2322, 2940, 3337
BspMAI CTGCAG 3 cut(s) 1168, 2244, 2313
BspMI ACCTGC 1 cut(s) 2786
BspPI GGATC 7 cut(s) 1468, 1854, 1871, 2474, 2513, 2556, 3185
BspQI GCTCTTC 2 cut(s) 1628, 2131
BspT107I GGYRCC 2 cut(s) 2938, 3335
BspTNI GGTCTC 3 cut(s) 1991, 2768, 3253
BsrBI CCGCTC 1 cut(s) 27
BsrDI GCAATG 1 cut(s) 3398
BsrFI RCCGGY 4 cut(s) 164, 188, 301, 2141
BssAI RCCGGY 4 cut(s) 164, 188, 301, 2141
BssECI CCNNGG 5 cut(s) 115, 849, 1697, 2957, 3060
BssNI GRCGYC 2 cut(s) 210, 2234
BssT1I CCWWGG 3 cut(s) 849, 1697, 2957
Bst2UI CCWGG 9 cut(s) 221, 353, 1485, 1794, 2101, 2157, 2841, 3061, 3333
Bst4CI ACNGT 5 cut(s) 1654, 1694, 2395, 2474, 3475
Bst6I CTCTTC 5 cut(s) 1628, 2125, 2131, 2721, 3620
BstACI GRCGYC 2 cut(s) 210, 2234
BstAPI GCANNNNNTGC 1 cut(s) 3400
BstBAI YACGTR 1 cut(s) 216
BstC8I GCNNGC 9 cut(s) 170, 190, 249, 303, 878, 957, 1899, 2503, 2652
BstDSI CCRYGG 2 cut(s) 849, 2957
BstENI CCTNNNNNAGG 1 cut(s) 2300
BstF5I GGATG 9 cut(s) 125, 567, 646, 1666, 1831, 1861, 2096, 2752, 3079
BstFNI CGCG 3 cut(s) 247, 1240, 1341
BstH2I RGCGCY 1 cut(s) 3669
BstHHI GCGC 2 cut(s) 888, 3668
BstMAI GTCTC 5 cut(s) 1716, 1991, 2250, 2768, 3253
BstMCI CGRYCG 3 cut(s) 101, 297, 2146
BstNI CCWGG 9 cut(s) 221, 353, 1485, 1794, 2101, 2157, 2841, 3061, 3333
BstNSI RCATGY 2 cut(s) 1316, 1763
BstSFI CTRYAG 4 cut(s) 1102, 1164, 2240, 2309
BstUI CGCG 3 cut(s) 247, 1240, 1341
BstV2I GAAGAC 2 cut(s) 234, 3261
BstX2I RGATCY 6 cut(s) 1876, 2386, 2479, 2518, 2548, 3190
BstXI CCANNNNNNTGG 2 cut(s) 1484, 2958
BstYI RGATCY 6 cut(s) 1876, 2386, 2479, 2518, 2548, 3190
BstZI CGGCCG 2 cut(s) 98, 294
Bsu15I ATCGAT 1 cut(s) 444
BsuI GTATCC 1 cut(s) 3092
BsuRI GGCC 7 cut(s) 100, 141, 168, 296, 1792, 2505, 2583
BsuTUI ATCGAT 1 cut(s) 444
BtgI CCRYGG 2 cut(s) 849, 2957
BtrI CACGTC 1 cut(s) 1593
BtsCI GGATG 9 cut(s) 125, 567, 646, 1666, 1831, 1861, 2096, 2752, 3079
BtsI GCAGTG 2 cut(s) 2306, 2599
BtsIMutI CAGTG 3 cut(s) 2306, 2599, 3480
BveI ACCTGC 1 cut(s) 2786
Cac8I GCNNGC 9 cut(s) 170, 190, 249, 303, 878, 957, 1899, 2503, 2652
CaiI CAGNNNCTG 1 cut(s) 328
CciI TCATGA 4 cut(s) 126, 1333, 2526, 3114
CfoI GCGC 2 cut(s) 888, 3668
Cfr10I RCCGGY 4 cut(s) 164, 188, 301, 2141
Cfr13I GGNCC 3 cut(s) 379, 3329, 3468
ClaI ATCGAT 1 cut(s) 444
CpoI CGGWCCG 1 cut(s) 379
CseI GACGC 1 cut(s) 2242
CsiI ACCWGGT 1 cut(s) 1483
Csp6I GTAC 9 cut(s) 334, 349, 1060, 1116, 1453, 1690, 2665, 2906, 3299
CspCI CAANNNNNGTGG 2 cut(s) 3580, 3615
CspI CGGWCCG 1 cut(s) 379
CviQI GTAC 9 cut(s) 334, 349, 1060, 1116, 1453, 1690, 2665, 2906, 3299
DrdI GACNNNNNNGTC 1 cut(s) 1708
DriI GACNNNNNGTC 1 cut(s) 3607
DseDI GACNNNNNNGTC 1 cut(s) 1708
EaeI YGGCCR 4 cut(s) 98, 139, 294, 2581
EagI CGGCCG 2 cut(s) 98, 294
Eam1104I CTCTTC 5 cut(s) 1628, 2125, 2131, 2721, 3620
Eam1105I GACNNNNNGTC 1 cut(s) 3607
EarI CTCTTC 5 cut(s) 1628, 2125, 2131, 2721, 3620
EciI GGCGGA 3 cut(s) 175, 269, 295
EclXI CGGCCG 2 cut(s) 98, 294
Eco130I CCWWGG 3 cut(s) 849, 1697, 2957
Eco147I AGGCCT 1 cut(s) 2505
Eco24I GRGCYC 1 cut(s) 828
Eco31I GGTCTC 3 cut(s) 1991, 2768, 3253
Eco32I GATATC 2 cut(s) 1544, 2106
Eco47I GGWCC 3 cut(s) 379, 3329, 3468
Eco47III AGCGCT 1 cut(s) 3667
Eco52I CGGCCG 2 cut(s) 98, 294
Eco57I CTGAAG 5 cut(s) 848, 2375, 3104, 3273, 3570
Eco72I CACGTG 1 cut(s) 216
EcoNI CCTNNNNNAGG 1 cut(s) 2300
EcoRI GAATTC 4 cut(s) 181, 858, 2920, 2989
EcoRII CCWGG 9 cut(s) 219, 351, 1483, 1792, 2099, 2155, 2839, 3059, 3331
EcoRV GATATC 2 cut(s) 1544, 2106
EcoT14I CCWWGG 3 cut(s) 849, 1697, 2957
EcoT38I GRGCYC 1 cut(s) 828
ErhI CCWWGG 3 cut(s) 849, 1697, 2957
FalI AAGNNNNNCTT 6 cut(s) 702, 734, 1344, 1376, 2714, 2746
FaqI GGGAC 4 cut(s) 46, 596, 819, 1247
FauI CCCGC 3 cut(s) 80, 621, 3359
FauNDI CATATG 4 cut(s) 2062, 2620, 3139, 3606
FbaI TGATCA 3 cut(s) 535, 2596, 2761
FblI GTMKAC 2 cut(s) 1287, 2269
FokI GGATG 9 cut(s) 132, 574, 653, 1673, 1838, 1848, 2083, 2759, 3086
FriOI GRGCYC 1 cut(s) 828
FspBI CTAG 5 cut(s) 1125, 1529, 1881, 1920, 2963
FspI TGCGCA 1 cut(s) 887
GlaI GCGC 2 cut(s) 887, 3667
GsaI CCCAGC 1 cut(s) 332
GsuI CTGGAG 2 cut(s) 1538, 2692
HaeII RGCGCY 1 cut(s) 3669
HaeIII GGCC 7 cut(s) 100, 141, 168, 296, 1792, 2505, 2583
HgaI GACGC 1 cut(s) 2242
HhaI GCGC 2 cut(s) 888, 3668
Hin1I GRCGYC 2 cut(s) 210, 2234
Hin6I GCGC 2 cut(s) 886, 3666
HinP1I GCGC 2 cut(s) 886, 3666
HincII GTYRAC 2 cut(s) 465, 1288
HindII GTYRAC 2 cut(s) 465, 1288
HindIII AAGCTT 3 cut(s) 874, 1617, 3182
HpaI GTTAAC 1 cut(s) 465
HphI GGTGA 7 cut(s) 14, 146, 154, 194, 1034, 2307, 2992
Hpy166II GTNNAC 7 cut(s) 465, 1288, 2255, 2270, 2860, 3068, 3471
Hpy8I GTNNAC 7 cut(s) 465, 1288, 2255, 2270, 2860, 3068, 3471
Hpy99I CGWCG 2 cut(s) 109, 1395
HpyAV CCTTC 9 cut(s) 25, 511, 914, 1211, 1837, 2539, 3011, 3519, 3529
HpyCH4III ACNGT 5 cut(s) 1654, 1694, 2395, 2474, 3475
HpyCH4IV ACGT 3 cut(s) 210, 215, 1592
HpySE526I ACGT 3 cut(s) 210, 215, 1592
Hsp92I GRCGYC 2 cut(s) 210, 2234
HspAI GCGC 2 cut(s) 886, 3666
Kpn2I TCCGGA 1 cut(s) 1729
KroI GCCGGC 2 cut(s) 188, 301
KroNI GCCGGC 2 cut(s) 190, 303
Ksp22I TGATCA 3 cut(s) 535, 2596, 2761
KspAI GTTAAC 1 cut(s) 465
LguI GCTCTTC 2 cut(s) 1628, 2131
LmnI GCTCC 6 cut(s) 114, 285, 823, 1471, 3039, 3430
LweI GCATC 9 cut(s) 110, 688, 921, 1651, 2056, 2185, 2520, 2737, 3282
MabI ACCWGGT 1 cut(s) 1483
MaeI CTAG 5 cut(s) 1125, 1529, 1881, 1920, 2963
MaeII ACGT 3 cut(s) 210, 215, 1592
MaeIII GTNAC 8 cut(s) 160, 200, 211, 1199, 1306, 2165, 2313, 3371
MbiI CCGCTC 1 cut(s) 27
MfeI CAATTG 1 cut(s) 2213
MflI RGATCY 6 cut(s) 1876, 2386, 2479, 2518, 2548, 3190
MhlI GDGCHC 5 cut(s) 828, 1468, 1510, 3036, 3561
MlyI GAGTC 7 cut(s) 368, 801, 1696, 1718, 1727, 2275, 2705
MmeI TCCRAC 2 cut(s) 1703, 2609
MroI TCCGGA 1 cut(s) 1729
MroNI GCCGGC 2 cut(s) 188, 301
MroXI GAANNNNTTC 6 cut(s) 974, 1356, 2284, 2726, 2848, 3171
MseI TTAA 8 cut(s) 464, 983, 1430, 1458, 1550, 1746, 3149, 3566
MslI CAYNNNNRTG 3 cut(s) 56, 690, 2956
MspA1I CMGCKG 2 cut(s) 277, 3391
MunI CAATTG 1 cut(s) 2213
MvaI CCWGG 9 cut(s) 221, 353, 1485, 1794, 2101, 2157, 2841, 3061, 3333
MvnI CGCG 3 cut(s) 247, 1240, 1341
NaeI GCCGGC 2 cut(s) 190, 303
NciI CCSGG 3 cut(s) 383, 413, 2122
NcoI CCATGG 2 cut(s) 849, 2957
NdeI CATATG 4 cut(s) 2062, 2620, 3139, 3606
NgoMIV GCCGGC 2 cut(s) 188, 301
NlaIV GGNNCC 4 cut(s) 825, 2322, 2940, 3337
NmuCI GTSAC 4 cut(s) 160, 200, 211, 2313
NsbI TGCGCA 1 cut(s) 887
NspI RCATGY 2 cut(s) 1316, 1763
PagI TCATGA 4 cut(s) 126, 1333, 2526, 3114
PceI AGGCCT 1 cut(s) 2505
PciI ACATGT 1 cut(s) 1312
PciSI GCTCTTC 2 cut(s) 1628, 2131
PdiI GCCGGC 2 cut(s) 190, 303
PdmI GAANNNNTTC 6 cut(s) 974, 1356, 2284, 2726, 2848, 3171
PflMI CCANNNNNTGG 2 cut(s) 1958, 2438
PfoI TCCNGGA 2 cut(s) 2099, 2839
PleI GAGTC 7 cut(s) 367, 801, 1696, 1717, 1727, 2274, 2705
PmaCI CACGTG 1 cut(s) 216
PmlI CACGTG 1 cut(s) 216
PpsI GAGTC 7 cut(s) 367, 801, 1696, 1717, 1727, 2274, 2705
Ppu21I YACGTR 1 cut(s) 216
PscI ACATGT 1 cut(s) 1312
Psp6I CCWGG 9 cut(s) 219, 351, 1483, 1792, 2099, 2155, 2839, 3059, 3331
PspCI CACGTG 1 cut(s) 216
PspFI CCCAGC 1 cut(s) 328
PspGI CCWGG 9 cut(s) 219, 351, 1483, 1792, 2099, 2155, 2839, 3059, 3331
PspN4I GGNNCC 4 cut(s) 825, 2322, 2940, 3337
PspPI GGNCC 3 cut(s) 379, 3329, 3468
PstI CTGCAG 3 cut(s) 1168, 2244, 2313
PstNI CAGNNNCTG 1 cut(s) 328
PsuI RGATCY 6 cut(s) 1876, 2386, 2479, 2518, 2548, 3190
PvuII CAGCTG 1 cut(s) 3391
RsaI GTAC 9 cut(s) 335, 350, 1061, 1117, 1454, 1691, 2666, 2907, 3300
RsaNI GTAC 9 cut(s) 334, 349, 1060, 1116, 1453, 1690, 2665, 2906, 3299
RseI CAYNNNNRTG 3 cut(s) 56, 690, 2956
Rsr2I CGGWCCG 1 cut(s) 379
RsrII CGGWCCG 1 cut(s) 379
SalI GTCGAC 1 cut(s) 1286
SapI GCTCTTC 2 cut(s) 1628, 2131
SaqAI TTAA 8 cut(s) 464, 983, 1430, 1458, 1550, 1746, 3149, 3566
Sau96I GGNCC 3 cut(s) 379, 3329, 3468
ScaI AGTACT 2 cut(s) 2666, 2907
SchI GAGTC 7 cut(s) 368, 801, 1696, 1718, 1727, 2275, 2705
SduI GDGCHC 5 cut(s) 828, 1468, 1510, 3036, 3561
SexAI ACCWGGT 1 cut(s) 1483
SfaNI GCATC 9 cut(s) 110, 688, 921, 1651, 2056, 2185, 2520, 2737, 3282
SfcI CTRYAG 4 cut(s) 1102, 1164, 2240, 2309
SinI GGWCC 3 cut(s) 379, 3329, 3468
SmiMI CAYNNNNRTG 3 cut(s) 56, 690, 2956
SmlI CTYRAG 4 cut(s) 1614, 2741, 3161, 3340
SmoI CTYRAG 4 cut(s) 1614, 2741, 3161, 3340
SseBI AGGCCT 1 cut(s) 2505
SspI AATATT 1 cut(s) 1525
SspMI CTAG 5 cut(s) 1125, 1529, 1881, 1920, 2963
StuI AGGCCT 1 cut(s) 2505
StyI CCWWGG 3 cut(s) 849, 1697, 2957
TaaI ACNGT 5 cut(s) 1654, 1694, 2395, 2474, 3475
TaiI ACGT 3 cut(s) 213, 218, 1595
TaqII GACCGA 2 cut(s) 3273, 3456
TatI WGTACW 6 cut(s) 1059, 1115, 1452, 1689, 2664, 2905
TauI GCSGC 6 cut(s) 103, 254, 280, 296, 1243, 3545
Tru1I TTAA 8 cut(s) 464, 983, 1430, 1458, 1550, 1746, 3149, 3566
Tru9I TTAA 8 cut(s) 464, 983, 1430, 1458, 1550, 1746, 3149, 3566
TscAI CASTG 3 cut(s) 2313, 2599, 3480
TseFI GTSAC 4 cut(s) 160, 200, 211, 2313
Tsp45I GTSAC 4 cut(s) 160, 200, 211, 2313
TspGWI ACGGA 1 cut(s) 278
TspRI CASTG 3 cut(s) 2313, 2599, 3480
Van91I CCANNNNNTGG 2 cut(s) 1958, 2438
VpaK11BI GGWCC 3 cut(s) 379, 3329, 3468
XagI CCTNNNNNAGG 1 cut(s) 2300
XapI RAATTY 7 cut(s) 181, 745, 858, 2677, 2920, 2946, 2989
XbaI TCTAGA 1 cut(s) 1528
XceI RCATGY 2 cut(s) 1316, 1763
XcmI CCANNNNNNNNNTGG 1 cut(s) 3067
XmiI GTMKAC 2 cut(s) 1287, 2269
XmnI GAANNNNTTC 6 cut(s) 974, 1356, 2284, 2726, 2848, 3171
XspI CTAG 5 cut(s) 1125, 1529, 1881, 1920, 2963
ZraI GACGTC 1 cut(s) 211
ZrmI AGTACT 2 cut(s) 2666, 2907
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.