FvH4_5g16860

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0013882
Physical Location & Seq
Forward (+)
9629867 .. 9638035
8169 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g16860.t1

Sequence Viewer

Length: 3651 bp
ATGCCTTGCTCCGCCTTCTTAATCCGCACCACCGCCACCATGTCCCTCAAACCCCGCCTCCGCCACTGCCGCCACGTCGCCCTACTCCGCCCCTCGCTTCTCCTCTCTTGCTTCATGAACACCAACAACGCCCTCTACCACACCCACTCCGTCACCGGCTTGCTCCGATGGAACTCCGCCCAGGCCGCTGACGTCGTGTGCATGTCATCTTCGCGTGCGGCGGAGAGGTCGACGGATTGGAAGCCCGCGCGGCGGCGGAAGGACGCCGCCGTGCCGAGCTGGTGCCACCAGCGGCAGCAGGGGTACGGGAGATTTGCGTACCAGGACGCCTCGGCTAGTGAAGATTCGGACCGTGAATTCGGGTCGTCGGGTCAGAGTTCTCGCCGGGAAATGACTGGTGCGACTCTTGAAAACATCGATGAGTGGAGATGGAAGTTGACCATGCTTGTTCGCAACAAAGATGAGCAAGAAGTGGTGTCGAGGGAAAGGAAGGACAGACGTGATTTTGATCATCTTGCAGAACTGGCAAGAGGGATGGGTTTATATAGCCGTCAGTATTCAAAGGTTGTAGTCTTTAGTAAAGTCCCTCAGCCAAATTACAGACCTGATCTGGATGATAGGCGACCACAGAGAGAGGTCGTCTTACCTTTTGGACTGCATAAAGATGTTGATGCTCATCTCAGAGCTCATCTTTCTCAAAAGCCAATGAACAGAGGAAATTTGTCGCATAATTCAATGTCAAGGTCAAATGGTAATGGAAGTATAGCTAAAAATGGGGGACTCTACGAGCAGGAGGAACCCTTAATACAGAATAGTGTTGCCATGGAGAGAATTCTTCAGCAGAGAAGCTTGCGATTGCGGAATAAGCAACAAGAATGGCAGGAATCTGTCGAAGGTCAAAAGATGCTTGAACTTCGTAGAAGTCTGCCTGCATATAAGGAGAAAGATTTCTTGTTAAAAGCCGTATCTGAAAATCAGGTCATAGTTGTCTCAGGTGAAACAGGTTGTGGTAAAACTACACAACTTCCTCAGTACATTTTAGAGTCTGAGATTGAAGCTGGTCGTGGAGGTGTCTGTAGTATTATTTGTACTCAACCTAGACGAATATCTGCCATGTCTGTTTCTGAAAGAGTTGCTGCAGAACGAGGGGAGAATCTGGGGGAATCAGTTGGTTATAAAGTTAGGCTGGAAGGAATGAAGGGCAGGGACACGCGGCTTCTTTTTTGCACCACAGGCATACTATTGAGGAGATTACTTGTTGACAGAAAGTTGAAAGGTGTTACACATGTCATTGTTGATGAGATCCATGAACGTGGAATGAATGAAGATTTTCTTCTTATCATTCTGAAAGAACTTCTCTTGCATCGCCCTGAGTTGAGATTGATTTTGATGAGTGCAACTTTAAATGCTGAGCTTTTCTCCTCGTACTTTAATGGTGCTCCAATGATCCATATACCTGGTTTTACATACCCAGTCCGAGCACATTTTCTGGAGAATATTTTAGAAATGACTGGATATCGGTTAAATCAATATAATCAAATTGATGATTATGGTCAAGATAAGACGTGGAAAATGCAGAAACAAGCTCAAGCTTTCAAAAAGAGGAAGAGTCAAATTGCATCTACCGTTGAGGATGCACTTGAAGCTGCTGACTTTAGGGGGTACAGTCCAAGGACTCAGGAGTCTCTTTCATGTTGGAACCCGGACTCCATTGGTTTTAATCTTATTGAACACGTGCTTTGCCACATAGTCAGGAAAGAACGACCTGGTGCTGTTTTGATTTTTATGACTGGTTGGGATGACATAAACTCCTTGAAGGATCAACTCCAATCTCATCCTCTTTTAGGAGATCCTAATAGGGTCCTGCTGCTTGCATGTCATGGTTCCATGCCTAGTGCTGAACAGAGGTTGATATTTGATAAACCAGAAGATGGGGTTAGGAAAATCGTTCTGGCTACAAACATGGCTGAGACCAGTATCACTATCAATGATGTAGTATTTGTGATTGATTGTGGAAAGGCGAAAGAAACATCATATGATGCACTCAATAACACTCCCTGTTTGCTTCCATCCTGGATATCAAAGGCTGCGTCCCGGCAGAGAAGGGGAAGAGCCGGTCGTGTTCAACCTGGCGAGTGTTACCATCTTTATCCCCGATGTGTATATGATGCCTTTGCTGATTATCAATTGCCTGAACTTTTGAGGACGCCTCTGCAGTCTCTATGTTTACAGATCAAGAGTCTACAGCTTGGAAGTATTGCTGAGTTCCTTTCTAAGGCACTGCAGTCACCGGAACCATTGTCGGTTCAAAATGCTGTTGATTATTTGAAGATCATTGGGGCTTTAGATGAAAATGAAGATCTCACAGTATTAGGTCGCCATCTGTCAACGCTTCCGGTTGAGCCAAAACTTGGGAAAATGCTTATATTAGGGGCTATCTTCAACTGTTTAGATCCAATAATGACTATTGTTGCAGGCCTGAGTATGAGAGATCCATTCATGATGCCATATGACAAGAAGGATCTTGCGGAATCGGCAAAAGCGCAATTCGCTGGCCGTGATAGCAGTGATCATCTTGCTCTTATCCGAGCATATGATGGCTGGAAAAATGCTGAAAGATCACAATCTGGTTATGAGTACTGCTGGAGAAATTTTCTTTCTGCACAAACTCTAAAAGCCATTGACTCTCTTCGGAAGCAGTTCTTCTTTTTGCTCAAGGATGCAGGTTTGGTTGACAACACCGAGAACTGTAATACATTGAGCCATGATGAACATCTTATCCGAGCAATCATCTGTGCGGGATTATTTCCTGGAATATGTTCTGTTGTGAACAAAGAGAAGTCCATTTCGTTGAAAACGATGGAAGATGGACAAGTACTTCTATACTCGAATTCAGTCAATGCTACGGTGCCTAAAATTCCATATCCATGGCTAGTGTTCAATGAAAAGGTGAAAGTGAATTCAGTATTCATCCGGGACTCAACTGGTGTATCTGATTCTGTGCTGCTTTTGTTTGGAGGGAACATTTCCAGGGGTGGGCTGGATGGACACCTGAAGATGTTGGGAGGATACTTGGAGTTTTTCATGAATCCTGCATTAGCAAATACATATGTTAGCTTGAAGAGGGAACTTGAGGAACTGATTCATAATAAGCTTCTAGATCCCAAATCGGACATGCAATCTCATAATAACCTCCTAGCAGCCCTAAGATTACTGGTCTCTGAAGACCGATGTGATGGAAGATTTGTCTATGGCCGCAAGATGCCAGTACCCTCCAAGAAGATAACAAAAGAGATAGGACCAGGCACCTTGAGGGTTCGCGATAATGGTAATAGTGGGGGTAATAATTCCAAAAGTCAGCTGCAAACATTGCTTGTCAGAGTAGGTCATGAAGCTCCCACCTACAAAACCAAACAATTGAAAAACAACCAGTTTTGTTCCACAGTGATCTTCAACGGGTTGAACTTTGTAGGGGAGCCTCGCAATAGTAAGAAAGAAGCAGAGAAGGAAGCAGCTGCTGAAGCTGTGCTCTGGTTAAAGGGCGAGAATCATTCATCTTCCAGAGATATTGACCATATGTCAATGCTTCTAAAGAAGAGTACAAAGAAAATCCGTAGAACGACCTCATTAGATATTGCCAAGTGGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001047 GO:0001067 GO:0001503 GO:0001817 GO:0001819 GO:0002151 GO:0002791 GO:0002793 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003697 GO:0003723 GO:0003724 GO:0003725 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006355 GO:0006357 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0008186 GO:0009605 GO:0009607 GO:0009615 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010501 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010638 GO:0014070 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0019899 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031330 GO:0032204 GO:0032206 GO:0032479 GO:0032481 GO:0032501 GO:0032647 GO:0032727 GO:0032879 GO:0032880 GO:0033043 GO:0033044 GO:0034641 GO:0042221 GO:0042623 GO:0042826 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043330 GO:0043331 GO:0043487 GO:0043489 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050707 GO:0050708 GO:0050714 GO:0050715 GO:0050789 GO:0050794 GO:0050896 GO:0051046 GO:0051047 GO:0051049 GO:0051050 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051222 GO:0051223 GO:0051239 GO:0051240 GO:0051252 GO:0051253 GO:0051254 GO:0051704 GO:0051707 GO:0051880 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070035 GO:0070201 GO:0071704 GO:0080090 GO:0090087 GO:0090304 GO:0090669 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1902369 GO:1902680 GO:1902739 GO:1902741 GO:1903506 GO:1903508 GO:1903530 GO:1903532 GO:1904951 GO:1990837 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind dsrm

Protein Analysis

1217

Amino Acids

137.2

Weight (kDa)

9.07

Isoelectric Point (pI)

47.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 320 - 471 3.2e-10 DEAD/DEAH box helicase
Helicase_C PF00271 579 - 708 6.5e-13 Helicase conserved C-terminal domain
WHD_HA2 PF04408 772 - 800 7.9e-08 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 801 - 861 2.2e-14 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 927 - 1009 7.5e-20 Oligonucleotide/oligosaccharide-binding (OB)-fold
RNA_hel_CTD PF26026 1017 - 1075 2.6e-18 C-terminal domain in RNA helicases
dsrm PF00035 1118 - 1179 1.2e-06 Double-stranded RNA binding motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013882)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G48650 AT1G48650
fragaria_vesca FvH4_5g16860
malus_domestica MD06G1222500.v1.1 MD14G1231600.v1.1
prunus_persica Prupe.5G227100_v2.0.a1
pyrus_communis pycom06g19810 pycom06g19820 pycom14g19280
rosa_chinensis RchiOBHm_Chr7g0179211
rosa_laevigata RLG00000005374
rosa_multiflora Rmu_sc0009798.1_g000005 Rmu_sc0021799.1_g000001
rosa_roxburghii Rroxscaffold_3G00274220
rosa_rugosa Rorug06G0422200
rosa_samantha Rh7BG021500
rosa_wichuraiana Rw7G001770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1176
AasI GACNNNNNNGTC 1 cut(s) 1681
AatII GACGTC 1 cut(s) 195
Acc36I ACCTGC 1 cut(s) 2714
AccB1I GGYRCC 3 cut(s) 282, 2908, 3305
AccB7I CCANNNNNTGG 2 cut(s) 1931, 2411
AccI GTMKAC 2 cut(s) 230, 2242
AccII CGCG 5 cut(s) 214, 248, 250, 1213, 3321
AclWI GGATC 8 cut(s) 1297, 1441, 1827, 1844, 2447, 2486, 2529, 3155
AcoI YGGCCR 2 cut(s) 2554, 3253
AcsI RAATTY 7 cut(s) 356, 718, 831, 2650, 2890, 2916, 2959
AcuI CTGAAG 4 cut(s) 821, 3074, 3243, 3540
AcvI CACGTG 1 cut(s) 1735
AcyI GRCGYC 4 cut(s) 192, 264, 327, 2207
AfiI CCNNNNNNNGG 6 cut(s) 252, 1844, 1931, 2275, 2411, 3036
AflIII ACRYGT 2 cut(s) 1285, 1732
AhdI GACNNNNNGTC 1 cut(s) 3577
AjiI CACGTC 3 cut(s) 76, 500, 1566
AjnI CCWGG 9 cut(s) 180, 321, 1456, 1765, 2072, 2128, 2809, 3029, 3301
AloI GAACNNNNNNTCC 4 cut(s) 1691, 1723, 2763, 2795
Alw21I GWGCWC 4 cut(s) 688, 1441, 1483, 3531
Alw26I GTCTC 5 cut(s) 994, 1689, 1964, 2223, 3223
AlwI GGATC 8 cut(s) 1297, 1441, 1827, 1844, 2447, 2486, 2529, 3155
AlwNI CAGNNNCTG 1 cut(s) 3518
AoxI GGCC 4 cut(s) 183, 2476, 2554, 3253
ApoI RAATTY 7 cut(s) 356, 718, 831, 2650, 2890, 2916, 2959
ArsI GACNNNNNNTTYG 4 cut(s) 341, 373, 2075, 2107
Asp700I GAANNNNTTC 5 cut(s) 947, 1329, 2699, 2818, 3141
AspLEI GCGC 2 cut(s) 250, 2545
AspS9I GGNCC 3 cut(s) 349, 1861, 3299
AsuC2I CCSGG 4 cut(s) 386, 1703, 2095, 2975
AsuHPI GGTGA 4 cut(s) 145, 1007, 2280, 2962
AvaII GGWCC 3 cut(s) 349, 1861, 3299
BaeI ACNNNNGTAYC 2 cut(s) 2973, 3006
BanI GGYRCC 3 cut(s) 282, 2908, 3305
BanII GRGCYC 1 cut(s) 688
BbrPI CACGTG 1 cut(s) 1735
BbsI GAAGAC 1 cut(s) 3231
Bbv12I GWGCWC 4 cut(s) 688, 1441, 1483, 3531
BbvCI CCTCAGC 1 cut(s) 588
BceAI ACGGC 4 cut(s) 254, 534, 947, 2541
BciT130I CCWGG 9 cut(s) 182, 323, 1458, 1767, 2074, 2130, 2811, 3031, 3303
BciVI GTATCC 1 cut(s) 3062
BclI TGATCA 2 cut(s) 508, 2569
BcnI CCSGG 4 cut(s) 386, 1703, 2095, 2975
BcoDI GTCTC 5 cut(s) 994, 1689, 1964, 2223, 3223
BfaI CTAG 6 cut(s) 336, 1098, 1893, 2933, 3158, 3197
BfmI CTRYAG 5 cut(s) 1075, 1137, 2213, 2243, 2282
BfuAI ACCTGC 1 cut(s) 2714
BfuI GTATCC 1 cut(s) 3062
BglI GCCNNNNNGGC 1 cut(s) 250
BglII AGATCT 1 cut(s) 2359
BlpI GCTNAGC 1 cut(s) 1410
BmcAI AGTACT 2 cut(s) 2639, 2877
Bme18I GGWCC 3 cut(s) 349, 1861, 3299
BmeRI GACNNNNNGTC 1 cut(s) 3577
BmgBI CACGTC 3 cut(s) 76, 500, 1566
BmgT120I GGNCC 3 cut(s) 349, 1861, 3299
BmiI GGNNCC 9 cut(s) 284, 798, 1700, 1862, 1885, 2295, 2910, 3307, 3477
BmrI ACTGGG 1 cut(s) 1466
BmuI ACTGGG 1 cut(s) 1466
BpiI GAAGAC 1 cut(s) 3231
BplI GAGNNNNNCTC 4 cut(s) 1403, 1435, 2194, 2226
BpmI CTGGAG 2 cut(s) 1511, 2665
Bpu10I CCTNAGC 1 cut(s) 588
Bpu1102I GCTNAGC 1 cut(s) 1410
BpuEI CTTGAG 4 cut(s) 1572, 2699, 3152, 3331
BpuMI CCSGG 4 cut(s) 386, 1703, 2095, 2975
Bsa29I ATCGAT 1 cut(s) 417
BsaAI YACGTR 1 cut(s) 1735
BsaBI GATNNNNATC 4 cut(s) 507, 675, 2623, 2772
BsaHI GRCGYC 4 cut(s) 192, 264, 327, 2207
BsaI GGTCTC 2 cut(s) 1964, 3223
BsaJI CCNNGG 6 cut(s) 180, 330, 822, 1670, 2927, 3030
BsaWI WCCGGW 2 cut(s) 2290, 2395
BsaXI ACNNNNNCTCC 8 cut(s) 42, 72, 131, 161, 1691, 1721, 1793, 1823
Bsc4I CCNNNNNNNGG 6 cut(s) 252, 1844, 1931, 2275, 2411, 3036
Bse118I RCCGGY 2 cut(s) 155, 2114
Bse3DI GCAATG 1 cut(s) 3368
Bse8I GATNNNNATC 4 cut(s) 507, 675, 2623, 2772
BseBI CCWGG 9 cut(s) 182, 323, 1458, 1767, 2074, 2130, 2811, 3031, 3303
BseCI ATCGAT 1 cut(s) 417
BseDI CCNNGG 6 cut(s) 180, 330, 822, 1670, 2927, 3030
BseGI GGATG 9 cut(s) 540, 619, 1639, 1804, 1834, 2069, 2725, 2970, 3049
BseJI GATNNNNATC 4 cut(s) 507, 675, 2623, 2772
BseLI CCNNNNNNNGG 6 cut(s) 252, 1844, 1931, 2275, 2411, 3036
BseMI GCAATG 1 cut(s) 3368
BseRI GAGGAG 3 cut(s) 92, 1261, 1411
BsgI GTGCAG 1 cut(s) 2646
Bsh1236I CGCG 5 cut(s) 214, 248, 250, 1213, 3321
Bsh1285I CGRYCG 1 cut(s) 2119
BshFI GGCC 4 cut(s) 185, 2478, 2556, 3255
BshNI GGYRCC 3 cut(s) 282, 2908, 3305
BshVI ATCGAT 1 cut(s) 417
BsiEI CGRYCG 1 cut(s) 2119
BsiHKAI GWGCWC 4 cut(s) 688, 1441, 1483, 3531
BsiSI CCGG 8 cut(s) 156, 385, 1703, 2095, 2115, 2291, 2396, 2974
BslFI GGGAC 6 cut(s) 28, 569, 792, 1220, 2077, 2990
BslI CCNNNNNNNGG 6 cut(s) 252, 1844, 1931, 2275, 2411, 3036
BsmAI GTCTC 5 cut(s) 994, 1689, 1964, 2223, 3223
BsmFI GGGAC 6 cut(s) 28, 569, 792, 1220, 2077, 2990
BsnI GGCC 4 cut(s) 185, 2478, 2556, 3255
Bso31I GGTCTC 2 cut(s) 1964, 3223
Bsp1286I GDGCHC 4 cut(s) 688, 1441, 1483, 3531
Bsp1720I GCTNAGC 1 cut(s) 1410
Bsp19I CCATGG 2 cut(s) 822, 2927
Bsp68I TCGCGA 1 cut(s) 3321
BspANI GGCC 4 cut(s) 185, 2478, 2556, 3255
BspDI ATCGAT 1 cut(s) 417
BspFNI CGCG 5 cut(s) 214, 248, 250, 1213, 3321
BspHI TCATGA 4 cut(s) 114, 2499, 3084, 3388
BspLI GGNNCC 9 cut(s) 284, 798, 1700, 1862, 1885, 2295, 2910, 3307, 3477
BspMAI CTGCAG 3 cut(s) 1141, 2217, 2286
BspMI ACCTGC 1 cut(s) 2714
BspPI GGATC 8 cut(s) 1297, 1441, 1827, 1844, 2447, 2486, 2529, 3155
BspQI GCTCTTC 1 cut(s) 2104
BspT107I GGYRCC 3 cut(s) 282, 2908, 3305
BspTNI GGTCTC 2 cut(s) 1964, 3223
BsrDI GCAATG 1 cut(s) 3368
BsrFI RCCGGY 2 cut(s) 155, 2114
BssAI RCCGGY 2 cut(s) 155, 2114
BssECI CCNNGG 6 cut(s) 180, 330, 822, 1670, 2927, 3030
BssNI GRCGYC 4 cut(s) 192, 264, 327, 2207
BssT1I CCWWGG 3 cut(s) 822, 1670, 2927
Bst2UI CCWGG 9 cut(s) 182, 323, 1458, 1767, 2074, 2130, 2811, 3031, 3303
Bst4CI ACNGT 8 cut(s) 353, 1627, 1667, 2368, 2447, 2750, 2908, 3445
Bst6I CTCTTC 5 cut(s) 1601, 2104, 2694, 3116, 3590
BstACI GRCGYC 4 cut(s) 192, 264, 327, 2207
BstAPI GCANNNNNTGC 1 cut(s) 3370
BstBAI YACGTR 1 cut(s) 1735
BstC8I GCNNGC 8 cut(s) 161, 216, 246, 851, 930, 1872, 2476, 2554
BstDSI CCRYGG 2 cut(s) 822, 2927
BstENI CCTNNNNNAGG 2 cut(s) 1842, 2273
BstF5I GGATG 9 cut(s) 540, 619, 1639, 1804, 1834, 2069, 2725, 2970, 3049
BstFNI CGCG 5 cut(s) 214, 248, 250, 1213, 3321
BstHHI GCGC 2 cut(s) 250, 2545
BstMAI GTCTC 5 cut(s) 994, 1689, 1964, 2223, 3223
BstMCI CGRYCG 1 cut(s) 2119
BstNI CCWGG 9 cut(s) 182, 323, 1458, 1767, 2074, 2130, 2811, 3031, 3303
BstNSI RCATGY 4 cut(s) 205, 1289, 1878, 3178
BstSFI CTRYAG 5 cut(s) 1075, 1137, 2213, 2243, 2282
BstUI CGCG 5 cut(s) 214, 248, 250, 1213, 3321
BstV2I GAAGAC 1 cut(s) 3231
BstX2I RGATCY 7 cut(s) 1302, 1849, 2359, 2452, 2491, 2521, 3160
BstXI CCANNNNNNTGG 3 cut(s) 1313, 1457, 2928
BstYI RGATCY 7 cut(s) 1302, 1849, 2359, 2452, 2491, 2521, 3160
Bsu15I ATCGAT 1 cut(s) 417
BsuI GTATCC 1 cut(s) 3062
BsuRI GGCC 4 cut(s) 185, 2478, 2556, 3255
BsuTUI ATCGAT 1 cut(s) 417
BtgI CCRYGG 2 cut(s) 822, 2927
BtgZI GCGATG 1 cut(s) 1349
BtrI CACGTC 3 cut(s) 76, 500, 1566
BtsCI GGATG 9 cut(s) 540, 619, 1639, 1804, 1834, 2069, 2725, 2970, 3049
BtsI GCAGTG 3 cut(s) 64, 2279, 2572
BtsIMutI CAGTG 4 cut(s) 64, 2279, 2572, 3450
BtuMI TCGCGA 1 cut(s) 3321
BveI ACCTGC 1 cut(s) 2714
Cac8I GCNNGC 8 cut(s) 161, 216, 246, 851, 930, 1872, 2476, 2554
CaiI CAGNNNCTG 1 cut(s) 3518
CciI TCATGA 4 cut(s) 114, 2499, 3084, 3388
CfoI GCGC 2 cut(s) 250, 2545
Cfr10I RCCGGY 2 cut(s) 155, 2114
Cfr13I GGNCC 3 cut(s) 349, 1861, 3299
ClaI ATCGAT 1 cut(s) 417
CpoI CGGWCCG 1 cut(s) 349
CseI GACGC 4 cut(s) 272, 335, 2079, 2215
CsiI ACCWGGT 2 cut(s) 1456, 1765
CspI CGGWCCG 1 cut(s) 349
DraI TTTAAA 1 cut(s) 1404
DrdI GACNNNNNNGTC 1 cut(s) 1681
DriI GACNNNNNGTC 1 cut(s) 3577
DseDI GACNNNNNNGTC 1 cut(s) 1681
EaeI YGGCCR 2 cut(s) 2554, 3253
Eam1104I CTCTTC 5 cut(s) 1601, 2104, 2694, 3116, 3590
Eam1105I GACNNNNNGTC 1 cut(s) 3577
EarI CTCTTC 5 cut(s) 1601, 2104, 2694, 3116, 3590
EciI GGCGGA 5 cut(s) 50, 77, 166, 236, 271
Ecl136II GAGCTC 1 cut(s) 686
Eco130I CCWWGG 3 cut(s) 822, 1670, 2927
Eco147I AGGCCT 1 cut(s) 2478
Eco24I GRGCYC 1 cut(s) 688
Eco31I GGTCTC 2 cut(s) 1964, 3223
Eco32I GATATC 2 cut(s) 1517, 2079
Eco47I GGWCC 3 cut(s) 349, 1861, 3299
Eco53kI GAGCTC 1 cut(s) 686
Eco57I CTGAAG 4 cut(s) 821, 3074, 3243, 3540
Eco72I CACGTG 1 cut(s) 1735
EcoICRI GAGCTC 1 cut(s) 686
EcoNI CCTNNNNNAGG 2 cut(s) 1842, 2273
EcoO109I RGGNCCY 1 cut(s) 1861
EcoRI GAATTC 4 cut(s) 356, 831, 2890, 2959
EcoRII CCWGG 9 cut(s) 180, 321, 1456, 1765, 2072, 2128, 2809, 3029, 3301
EcoRV GATATC 2 cut(s) 1517, 2079
EcoT14I CCWWGG 3 cut(s) 822, 1670, 2927
EcoT38I GRGCYC 1 cut(s) 688
ErhI CCWWGG 3 cut(s) 822, 1670, 2927
FalI AAGNNNNNCTT 4 cut(s) 1317, 1349, 2687, 2719
FaqI GGGAC 6 cut(s) 28, 569, 792, 1220, 2077, 2990
FauI CCCGC 3 cut(s) 62, 253, 2791
FauNDI CATATG 5 cut(s) 2035, 2509, 2593, 3109, 3576
FbaI TGATCA 2 cut(s) 508, 2569
FblI GTMKAC 2 cut(s) 230, 2242
FokI GGATG 9 cut(s) 547, 626, 1646, 1811, 1821, 2056, 2732, 2957, 3056
FriOI GRGCYC 1 cut(s) 688
FspBI CTAG 6 cut(s) 336, 1098, 1893, 2933, 3158, 3197
GlaI GCGC 2 cut(s) 249, 2544
GsuI CTGGAG 2 cut(s) 1511, 2665
HaeIII GGCC 4 cut(s) 185, 2478, 2556, 3255
HapII CCGG 8 cut(s) 156, 385, 1703, 2095, 2115, 2291, 2396, 2974
HgaI GACGC 4 cut(s) 272, 335, 2079, 2215
HhaI GCGC 2 cut(s) 250, 2545
Hin1I GRCGYC 4 cut(s) 192, 264, 327, 2207
Hin6I GCGC 2 cut(s) 248, 2543
HinP1I GCGC 2 cut(s) 248, 2543
HincII GTYRAC 5 cut(s) 231, 438, 1261, 2388, 2734
HindII GTYRAC 5 cut(s) 231, 438, 1261, 2388, 2734
HindIII AAGCTT 3 cut(s) 847, 1590, 3152
HpaII CCGG 8 cut(s) 156, 385, 1703, 2095, 2115, 2291, 2396, 2974
HphI GGTGA 4 cut(s) 145, 1007, 2280, 2962
Hpy166II GTNNAC 8 cut(s) 231, 438, 1261, 2228, 2243, 2388, 2734, 2830
Hpy8I GTNNAC 8 cut(s) 231, 438, 1261, 2228, 2243, 2388, 2734, 2830
Hpy99I CGWCG 4 cut(s) 80, 197, 235, 370
HpyCH4III ACNGT 8 cut(s) 353, 1627, 1667, 2368, 2447, 2750, 2908, 3445
HpyCH4IV ACGT 6 cut(s) 75, 192, 499, 1312, 1565, 1734
HpySE526I ACGT 6 cut(s) 75, 192, 499, 1312, 1565, 1734
Hsp92I GRCGYC 4 cut(s) 192, 264, 327, 2207
HspAI GCGC 2 cut(s) 248, 2543
Ksp22I TGATCA 2 cut(s) 508, 2569
LguI GCTCTTC 1 cut(s) 2104
LmnI GCTCC 5 cut(s) 14, 168, 1444, 3400, 3475
MabI ACCWGGT 2 cut(s) 1456, 1765
MaeI CTAG 6 cut(s) 336, 1098, 1893, 2933, 3158, 3197
MaeII ACGT 6 cut(s) 75, 192, 499, 1312, 1565, 1734
MaeIII GTNAC 4 cut(s) 151, 1279, 2138, 2286
MfeI CAATTG 2 cut(s) 2186, 3416
MflI RGATCY 7 cut(s) 1302, 1849, 2359, 2452, 2491, 2521, 3160
MhlI GDGCHC 4 cut(s) 688, 1441, 1483, 3531
MmeI TCCRAC 1 cut(s) 1676
MroXI GAANNNNTTC 5 cut(s) 947, 1329, 2699, 2818, 3141
MseI TTAA 8 cut(s) 20, 803, 956, 1403, 1431, 1523, 1719, 3536
MslI CAYNNNNRTG 3 cut(s) 663, 1311, 2926
MspA1I CMGCKG 4 cut(s) 188, 292, 3361, 3515
MspI CCGG 8 cut(s) 156, 385, 1703, 2095, 2115, 2291, 2396, 2974
MunI CAATTG 2 cut(s) 2186, 3416
MvaI CCWGG 9 cut(s) 182, 323, 1458, 1767, 2074, 2130, 2811, 3031, 3303
MvnI CGCG 5 cut(s) 214, 248, 250, 1213, 3321
NciI CCSGG 4 cut(s) 386, 1703, 2095, 2975
NcoI CCATGG 2 cut(s) 822, 2927
NdeI CATATG 5 cut(s) 2035, 2509, 2593, 3109, 3576
NlaIV GGNNCC 9 cut(s) 284, 798, 1700, 1862, 1885, 2295, 2910, 3307, 3477
NmeAIII GCCGAG 2 cut(s) 300, 311
NmuCI GTSAC 2 cut(s) 151, 2286
NruI TCGCGA 1 cut(s) 3321
NspI RCATGY 4 cut(s) 205, 1289, 1878, 3178
PagI TCATGA 4 cut(s) 114, 2499, 3084, 3388
PceI AGGCCT 1 cut(s) 2478
PciI ACATGT 1 cut(s) 1285
PciSI GCTCTTC 1 cut(s) 2104
PcsI WCGNNNNNNNCGW 1 cut(s) 2855
PdmI GAANNNNTTC 5 cut(s) 947, 1329, 2699, 2818, 3141
PfeI GAWTC 9 cut(s) 344, 884, 1153, 1163, 2531, 2996, 3088, 3142, 3547
PflMI CCANNNNNTGG 2 cut(s) 1931, 2411
PfoI TCCNGGA 3 cut(s) 2072, 2809, 2973
PmaCI CACGTG 1 cut(s) 1735
PmlI CACGTG 1 cut(s) 1735
Ppu21I YACGTR 1 cut(s) 1735
PpuMI RGGWCCY 1 cut(s) 1861
PscI ACATGT 1 cut(s) 1285
PsiI TTATAA 1 cut(s) 1176
Psp124BI GAGCTC 1 cut(s) 688
Psp5II RGGWCCY 1 cut(s) 1861
Psp6I CCWGG 9 cut(s) 180, 321, 1456, 1765, 2072, 2128, 2809, 3029, 3301
PspCI CACGTG 1 cut(s) 1735
PspGI CCWGG 9 cut(s) 180, 321, 1456, 1765, 2072, 2128, 2809, 3029, 3301
PspN4I GGNNCC 9 cut(s) 284, 798, 1700, 1862, 1885, 2295, 2910, 3307, 3477
PspPI GGNCC 3 cut(s) 349, 1861, 3299
PspPPI RGGWCCY 1 cut(s) 1861
PsrI GAACNNNNNNTAC 2 cut(s) 789, 821
PstI CTGCAG 3 cut(s) 1141, 2217, 2286
PstNI CAGNNNCTG 1 cut(s) 3518
PsuI RGATCY 7 cut(s) 1302, 1849, 2359, 2452, 2491, 2521, 3160
PvuII CAGCTG 2 cut(s) 3361, 3515
RruI TCGCGA 1 cut(s) 3321
RseI CAYNNNNRTG 3 cut(s) 663, 1311, 2926
Rsr2I CGGWCCG 1 cut(s) 349
RsrII CGGWCCG 1 cut(s) 349
SacI GAGCTC 1 cut(s) 688
SalI GTCGAC 1 cut(s) 229
SapI GCTCTTC 1 cut(s) 2104
SaqAI TTAA 8 cut(s) 20, 803, 956, 1403, 1431, 1523, 1719, 3536
Sau96I GGNCC 3 cut(s) 349, 1861, 3299
ScaI AGTACT 2 cut(s) 2639, 2877
SduI GDGCHC 4 cut(s) 688, 1441, 1483, 3531
SexAI ACCWGGT 2 cut(s) 1456, 1765
SfcI CTRYAG 5 cut(s) 1075, 1137, 2213, 2243, 2282
SinI GGWCC 3 cut(s) 349, 1861, 3299
SmiMI CAYNNNNRTG 3 cut(s) 663, 1311, 2926
SmlI CTYRAG 4 cut(s) 1587, 2714, 3131, 3310
SmoI CTYRAG 4 cut(s) 1587, 2714, 3131, 3310
SseBI AGGCCT 1 cut(s) 2478
SspI AATATT 1 cut(s) 1498
SspMI CTAG 6 cut(s) 336, 1098, 1893, 2933, 3158, 3197
SstI GAGCTC 1 cut(s) 688
StuI AGGCCT 1 cut(s) 2478
StyI CCWWGG 3 cut(s) 822, 1670, 2927
TaaI ACNGT 8 cut(s) 353, 1627, 1667, 2368, 2447, 2750, 2908, 3445
TaiI ACGT 6 cut(s) 78, 195, 502, 1315, 1568, 1737
TaqI TCGA 5 cut(s) 230, 417, 479, 891, 2888
TaqII GACCGA 1 cut(s) 3243
TatI WGTACW 5 cut(s) 1032, 1088, 2637, 2875, 3599
TauI GCSGC 9 cut(s) 72, 188, 221, 253, 256, 269, 295, 1216, 3258
TfiI GAWTC 9 cut(s) 344, 884, 1153, 1163, 2531, 2996, 3088, 3142, 3547
Tru1I TTAA 8 cut(s) 20, 803, 956, 1403, 1431, 1523, 1719, 3536
Tru9I TTAA 8 cut(s) 20, 803, 956, 1403, 1431, 1523, 1719, 3536
TscAI CASTG 4 cut(s) 71, 2286, 2572, 3450
TseFI GTSAC 2 cut(s) 151, 2286
Tsp45I GTSAC 2 cut(s) 151, 2286
TspGWI ACGGA 3 cut(s) 139, 248, 3602
TspRI CASTG 4 cut(s) 71, 2286, 2572, 3450
Van91I CCANNNNNTGG 2 cut(s) 1931, 2411
VpaK11BI GGWCC 3 cut(s) 349, 1861, 3299
XagI CCTNNNNNAGG 2 cut(s) 1842, 2273
XapI RAATTY 7 cut(s) 356, 718, 831, 2650, 2890, 2916, 2959
XbaI TCTAGA 1 cut(s) 3157
XceI RCATGY 4 cut(s) 205, 1289, 1878, 3178
XcmI CCANNNNNNNNNTGG 1 cut(s) 3037
XmiI GTMKAC 2 cut(s) 230, 2242
XmnI GAANNNNTTC 5 cut(s) 947, 1329, 2699, 2818, 3141
XspI CTAG 6 cut(s) 336, 1098, 1893, 2933, 3158, 3197
ZraI GACGTC 1 cut(s) 193
ZrmI AGTACT 2 cut(s) 2639, 2877
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.