Rw7G001770

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0013882
Physical Location & Seq
Reverse (-)
1443823 .. 1451783
7961 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G001770.1

Sequence Viewer

Length: 3690 bp
ATGCCTTACTCCGCCTTCTTCCACCGCTCTATAATAATCCGCACCACCTCCACCGCCATGTCCCTCAAACCCCGCCACCGCCACTTCTCCCTCCTCCGGCCGCCGTCCCTCCTCCTCCTCGGATGCTTCATGAACACCAACGGCCACTACCACACTCACCCCGTCACCGGCCTGCTCAAATGGAATTCCGCCGGCTGCTGCCGTCACGCTGACGTCACTTGCCTGGCCTCGTCCTCCTCGTCGTCGTCGTCGTCGCGTGCGGCGGAGAGGACGGATTGGAAGCAGCGGCGGAGGAGCGTTGCGGCCGTGCCGGCGTGGTACCAGCAGCAGCAGAAGCAGGGGTATGGGAGGTTTGCGTACCAGGACGAGTCGGCTAGTGAAGATTCGGACCGGGAGTTCGGGTCGGGTCAGAGCTCTCGCCGGGAAATGACTGGTTCGACCATTGACAACATCGATGAGTGGAGATGGAAGTTAACCTTGCTTGTTCGCAACAAAGATGAGCAAGAATTGGTGTCGAGGGAAAGGAAGGACAGGCGTGATTTTGATCATCTTTCAGAACTGGCAAGAGGGATGGGTTTATATAGCCGTCAGTATTCAAAGGTTGTAGTCTTTAGTAAAGTCCCGCAGCCAAATTACAGACCTGATCTGGATGATAGGCGACCACAAAGAGAGGTTGTCTTACCTTTTGGACTGCATAAAGATGTAGATGCTCATCTCAAAGCTCATCTTTCTCAAAAGCCAATGAGCAGAGGAAATTTATCGCATGCTTCAATGTCAAGGTCAAATAGTAATGGAAGTATAGCTAAAGACGGGGGACTCTACGAGCAGGAGGAGCCCTTGATACAGAATAGTGTTGCCATGGAGAGAATTCTTCAGCAGAAAAGCTTGCGATTGCTCAATAAGCAACAAGAATGGCAGGAATCCATCGAAGGTCAAAAGATGCTTGAACTTCGTAGAAGTCTGCCTGCATATAAAGAGAAAGATTCCTTGTTAAAAGCCATATCCGAAAATCAGGTCATAGTTGTCTCAGGTGAAACAGGTTGTGGTAAAACTACACAACTTCCTCAGTACATTTTAGAATCTGAGATTGAAGCTGGTCGTGGAGGTGTCTGTAGTATTATTTGTACTCAGCCTAGACGAATATCTGCCATGTCTGTTTCTGAAAGAGTTGCTGCAGAACGAGGGGAGAATCTGGGGGAATCAGTTGGTTACAAAGTTAGGCTGGAAGGAATGAAAGGGAGGGACACGCGGCTTCTTTTTTGCACCACAGGCATACTATTGAGAAGATTACTTGTCGACAGAAAGTTGAGAGGTGTTACACATGTCATTGTTGATGAGATTCATGAACGTGGAATGAATGAAGATTTTCTTCTTATCGTTCTGAAAGAACTTCTCTCTCGCCGTCCTGAGTTGAGATTGATTTTGATGAGTGCAACCTTAAATGCTGAGCTTTTCTCCTCGTACTTTAATGGTGCTCCAATGATCCATATACCTGGTTTTACATACCCGGTCCGAGCACATTTTCTGGAGAATATTCTAGAAATGACTGGATATCGGTTAAATCAATATAATCAAATTGATGATTATGGTCAAGATAAGACGTGGAAAATGCAGAAACAAGCTCAAGCTTTCAAAAAGAGGAAGAGCCAAATTGCTTCTACCGTTGAGGATGCACTTGAAGCTGCTGACTTTAGGGAGTACAGTCCAAGGACTCAGGAGTCTCTGTCCTGTTGGAATCCGGACTCCATTGGTTTTAATCTCATTGAGCATGTGCTTTGCCACATAGTCAGGAAAGAAAGGCCTGGTGCTATTTTGGTTTTTATGACTGGTTGGGATGACATAAACTCCTTGAAGGATCAACTCCAATCTCATCCCCTTTTAGGAGATCCTAGTAGGGTCCTGCTGCTTGCCTGTCATGGTTCCATGCCTAGCTCTGAACAGAGGTTGATATTTGATAAACCAGAAGATGGAGTTAGGAAAATCGTTCTGGCTACAAACATGGCTGAGACCAGTATCACTATCAATGATGTAGTATTTGTGATTGATTGTGGAAAGGCGAAAGAAACATCATATGATGCACTCAATAATACTCCCTGTTTGCTTCCATCCTGGATATCAAAGGCTGCTTCCCGGCAAAGAAGAGGAAGAGCTGGTCGTGTTCAACCCGGCGAGTGTTACCATCTATATCCCCAATGTGTATATGATGCCTTTGCTGATTATCAATTGCCTGAACTTTTGAGGACGCCTCTGCAGTCTCTATGTTTACAAATCAAGAGTCTACAACTTGGAAGCATTTCTGAGTTCCTTTCTAAGGCACTGCAGTCACCGGAACCTTTGTCGGTTCAAAATGCTGTTGATTATCTGAAGATCATAGGGGCTTTAGATGACAATGAAGATCTCACAGTATTAGGTCGCCACCTGTCAATGCTTCCTGTTGAGCCAAAACTTGGGAAAATGCTCATATTAGGGGCTATCTTCAACTGTTTAGATCCAATAATGACTATTGTTGCAGGCCTTAGTATGAGAGATCCATTCATGATGCCATTTGACAAGAAGGATCTTGCGGAATCAGCGAAAGCACAATTTTCTGGCCGTGATAACAGCGATCATCTGGCTCTTATCCGAGCATATGATGGTTGGAAAAATGCTGAAAGATCGCAAGCTGGTTATGAGTACTGCTGGAGAAATTTTCTTTCTGCACAAACTCTAAAAGCCATTGACTCTCTTCGGAAGCAGTTCTTCTTCTTGCTCAAGGATGCTGGTTTGGTTGATCACAACATTGAGAACTGCAATACATTGAGCTATGATGAACAGCTTATCCGAGCAATCATCTGTGCAGGATTATTTCCTGGAATATGTTCTGTTGTGAACAAAGAGAAGTCGATTTCGTTGAAAACAATGGAAGATGGACAAGTACTTCTATACTCGAATTCTGTCAATGCTTCGGTGCCTAAAATTCCATATCCATGGCTAGTTTTCAACGAAAAGGTGAAAGTGAATTCAGTATTCCTTCGGGATTCAACCGGCGTCTCTGATTCTGTGCTCCTTTTGTTTGGAGGCAACATTTCCAGGGGTGGACTGGATGGGCACCTGAAGATGTTGGGAGGATACTTGGAGTTTTTCATGAATCCTGCATTATCAAATACATATCTTAGCTTAAAGAGGGAACTTGAGGAACTGATTCATAATAAGCTTATAGATCCCAAATTGGATATGCAATCTCATACTAACCTCCTATCAGCCCTAAGATTACTGGTCTCTGAAGACAGATGTGATGGTAGATTTGTCTTTGGTCGCAAGATGCCAGTACCCTCAAAGAAGATAATGAAAGAGTTAGGTCCAGGCACCTTGAGGGTTGGCGATAATGGTAAAAGCGGGGGTAACAATTCCAAAAGTCAGCTGCAAACATTGCTTGTCAGAGCAGGACATGAAGCTCCCACCTACAAAACAAAACAACTGAAAAACAACCAGTTTCGGTCCACAGTGATCTTCAATGGGTTGAACTTTGTAGGGCAGCCTTCCAATAGTAAGAAACAAGCAGAGAAGGAAGCGGCTGCTGAAGCTGTGCTCTGGTTAAAGGGCGATAACCATTCATCTTCCACAGATATTGACCATATGTCAATGCTTCTAAAGAAGAGTAGAAAGAAAATCCATAAAACGACCTCATTTGATAGCGCTAAGTGGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001047 GO:0001067 GO:0001503 GO:0001817 GO:0001819 GO:0002151 GO:0002791 GO:0002793 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003697 GO:0003723 GO:0003724 GO:0003725 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006355 GO:0006357 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0008186 GO:0009605 GO:0009607 GO:0009615 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010501 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010638 GO:0014070 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0019899 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031330 GO:0032204 GO:0032206 GO:0032479 GO:0032481 GO:0032501 GO:0032647 GO:0032727 GO:0032879 GO:0032880 GO:0033043 GO:0033044 GO:0034641 GO:0042221 GO:0042623 GO:0042826 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043330 GO:0043331 GO:0043487 GO:0043489 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050707 GO:0050708 GO:0050714 GO:0050715 GO:0050789 GO:0050794 GO:0050896 GO:0051046 GO:0051047 GO:0051049 GO:0051050 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051222 GO:0051223 GO:0051239 GO:0051240 GO:0051252 GO:0051253 GO:0051254 GO:0051704 GO:0051707 GO:0051880 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070035 GO:0070201 GO:0071704 GO:0080090 GO:0090087 GO:0090304 GO:0090669 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1902369 GO:1902680 GO:1902739 GO:1902741 GO:1903506 GO:1903508 GO:1903530 GO:1903532 GO:1904951 GO:1990837 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind dsrm

Protein Analysis

1229

Amino Acids

138.57

Weight (kDa)

9.03

Isoelectric Point (pI)

51.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 330 - 483 2.4e-10 DEAD/DEAH box helicase
Helicase_C PF00271 591 - 720 9.1e-13 Helicase conserved C-terminal domain
WHD_HA2 PF04408 784 - 812 3.3e-08 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 813 - 873 2e-14 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 940 - 1022 9.9e-20 Oligonucleotide/oligosaccharide-binding (OB)-fold
RNA_hel_CTD PF26026 1030 - 1088 8.5e-18 C-terminal domain in RNA helicases
dsrm PF00035 1131 - 1192 1.1e-08 Double-stranded RNA binding motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013882)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G48650 AT1G48650
fragaria_vesca FvH4_5g16860
malus_domestica MD06G1222500.v1.1 MD14G1231600.v1.1
prunus_persica Prupe.5G227100_v2.0.a1
pyrus_communis pycom06g19810 pycom06g19820 pycom14g19280
rosa_chinensis RchiOBHm_Chr7g0179211
rosa_laevigata RLG00000005374
rosa_multiflora Rmu_sc0009798.1_g000005 Rmu_sc0021799.1_g000001
rosa_roxburghii Rroxscaffold_3G00274220
rosa_rugosa Rorug06G0422200
rosa_samantha Rh7BG021500
rosa_wichuraiana Rw7G001770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1717
AatII GACGTC 1 cut(s) 216
Acc65I GGTACC 1 cut(s) 318
AccB1I GGYRCC 4 cut(s) 318, 2947, 3087, 3344
AccB7I CCANNNNNTGG 2 cut(s) 1967, 2447
AccBSI CCGCTC 1 cut(s) 27
AccI GTMKAC 2 cut(s) 1296, 2278
AccII CGCG 2 cut(s) 256, 1249
AccIII TCCGGA 1 cut(s) 1738
AclWI GGATC 7 cut(s) 1477, 1863, 1880, 2483, 2522, 2565, 3194
AcoI YGGCCR 4 cut(s) 98, 142, 303, 2590
AcsI RAATTY 7 cut(s) 184, 754, 867, 2686, 2929, 2955, 2998
AcuI CTGAAG 5 cut(s) 857, 2384, 3113, 3282, 3579
AcyI GRCGYC 3 cut(s) 213, 2243, 3027
AfaI GTAC 9 cut(s) 320, 359, 1070, 1126, 1463, 1700, 2675, 2916, 3309
AfeI AGCGCT 1 cut(s) 3676
AfiI CCNNNNNNNGG 6 cut(s) 96, 167, 1880, 1967, 2311, 2447
AflIII ACRYGT 1 cut(s) 1321
AhdI GACNNNNNGTC 1 cut(s) 3616
AjiI CACGTC 1 cut(s) 1602
AjnI CCWGG 8 cut(s) 222, 360, 1492, 1801, 2108, 2848, 3068, 3340
AloI GAACNNNNNNTCC 4 cut(s) 380, 412, 2802, 2834
Alw21I GWGCWC 5 cut(s) 416, 1477, 1519, 3045, 3570
Alw26I GTCTC 6 cut(s) 1030, 1725, 2000, 2259, 3034, 3262
AlwI GGATC 7 cut(s) 1477, 1863, 1880, 2483, 2522, 2565, 3194
Aor13HI TCCGGA 1 cut(s) 1738
Aor51HI AGCGCT 1 cut(s) 3676
AoxI GGCC 8 cut(s) 98, 142, 169, 225, 303, 1799, 2512, 2590
ApoI RAATTY 7 cut(s) 184, 754, 867, 2686, 2929, 2955, 2998
ArsI GACNNNNNNTTYG 6 cut(s) 380, 412, 999, 1031, 2260, 2292
Asp700I GAANNNNTTC 5 cut(s) 1365, 2293, 2735, 2857, 3180
Asp718I GGTACC 1 cut(s) 318
AspLEI GCGC 1 cut(s) 3677
AspS9I GGNCC 5 cut(s) 388, 1510, 1897, 3338, 3477
AsuC2I CCSGG 5 cut(s) 392, 422, 1508, 2131, 2166
AsuHPI GGTGA 5 cut(s) 149, 157, 1043, 2316, 3001
AvaII GGWCC 5 cut(s) 388, 1510, 1897, 3338, 3477
BaeGI GKGCMC 1 cut(s) 3090
BanI GGYRCC 4 cut(s) 318, 2947, 3087, 3344
BanII GRGCYC 2 cut(s) 416, 837
BbsI GAAGAC 1 cut(s) 3270
Bbv12I GWGCWC 5 cut(s) 416, 1477, 1519, 3045, 3570
BceAI ACGGC 7 cut(s) 88, 157, 186, 290, 570, 1386, 2577
BciT130I CCWGG 8 cut(s) 224, 362, 1494, 1803, 2110, 2850, 3070, 3342
BciVI GTATCC 1 cut(s) 3101
BclI TGATCA 2 cut(s) 544, 2770
BcnI CCSGG 5 cut(s) 392, 422, 1508, 2131, 2166
BcoDI GTCTC 6 cut(s) 1030, 1725, 2000, 2259, 3034, 3262
BfaI CTAG 6 cut(s) 375, 1134, 1538, 1890, 1929, 2972
BfmI CTRYAG 4 cut(s) 1111, 1173, 2249, 2318
BfoI RGCGCY 1 cut(s) 3678
BfuI GTATCC 1 cut(s) 3101
BglI GCCNNNNNGGC 1 cut(s) 311
BglII AGATCT 1 cut(s) 2395
BlpI GCTNAGC 1 cut(s) 1446
BmcAI AGTACT 2 cut(s) 2675, 2916
Bme18I GGWCC 5 cut(s) 388, 1510, 1897, 3338, 3477
BmeRI GACNNNNNGTC 1 cut(s) 3616
BmgBI CACGTC 1 cut(s) 1602
BmgT120I GGNCC 5 cut(s) 388, 1510, 1897, 3338, 3477
BmiI GGNNCC 8 cut(s) 320, 834, 1898, 1921, 2331, 2949, 3089, 3346
BmsI GCATC 9 cut(s) 113, 697, 930, 1660, 2065, 2194, 2529, 2746, 3291
BpiI GAAGAC 1 cut(s) 3270
BplI GAGNNNNNCTC 4 cut(s) 1439, 1471, 2230, 2262
BpmI CTGGAG 2 cut(s) 1547, 2701
Bpu1102I GCTNAGC 1 cut(s) 1446
BpuEI CTTGAG 4 cut(s) 1608, 2735, 3191, 3370
BpuMI CCSGG 5 cut(s) 392, 422, 1508, 2131, 2166
Bsa29I ATCGAT 1 cut(s) 453
BsaBI GATNNNNATC 2 cut(s) 543, 711
BsaHI GRCGYC 3 cut(s) 213, 2243, 3027
BsaI GGTCTC 2 cut(s) 2000, 3262
BsaJI CCNNGG 5 cut(s) 118, 858, 1706, 2966, 3069
BsaWI WCCGGW 2 cut(s) 1738, 2326
BsaXI ACNNNNNCTCC 6 cut(s) 386, 416, 1709, 1739, 1829, 1859
Bsc4I CCNNNNNNNGG 6 cut(s) 96, 167, 1880, 1967, 2311, 2447
Bse118I RCCGGY 4 cut(s) 167, 191, 310, 3023
Bse1I ACTGG 9 cut(s) 436, 564, 1552, 1831, 2010, 3084, 3258, 3305, 3469
Bse3DI GCAATG 1 cut(s) 3407
Bse8I GATNNNNATC 2 cut(s) 543, 711
BseAI TCCGGA 1 cut(s) 1738
BseBI CCWGG 8 cut(s) 224, 362, 1494, 1803, 2110, 2850, 3070, 3342
BseCI ATCGAT 1 cut(s) 453
BseDI CCNNGG 5 cut(s) 118, 858, 1706, 2966, 3069
BseGI GGATG 9 cut(s) 128, 576, 655, 1675, 1840, 1870, 2105, 2761, 3088
BseJI GATNNNNATC 2 cut(s) 543, 711
BseLI CCNNNNNNNGG 6 cut(s) 96, 167, 1880, 1967, 2311, 2447
BseMI GCAATG 1 cut(s) 3407
BseMII CTCAG 9 cut(s) 1041, 1074, 1079, 1142, 1398, 1437, 1727, 1995, 2289
BseNI ACTGG 9 cut(s) 436, 564, 1552, 1831, 2010, 3084, 3258, 3305, 3469
BseRI GAGGAG 8 cut(s) 83, 101, 104, 107, 226, 307, 845, 1447
BseSI GKGCMC 1 cut(s) 3090
BseX3I CGGCCG 2 cut(s) 98, 303
BsgI GTGCAG 2 cut(s) 2682, 2856
Bsh1236I CGCG 2 cut(s) 256, 1249
Bsh1285I CGRYCG 2 cut(s) 101, 306
BshFI GGCC 8 cut(s) 100, 144, 171, 227, 305, 1801, 2514, 2592
BshNI GGYRCC 4 cut(s) 318, 2947, 3087, 3344
BshVI ATCGAT 1 cut(s) 453
BsiEI CGRYCG 2 cut(s) 101, 306
BsiHKAI GWGCWC 5 cut(s) 416, 1477, 1519, 3045, 3570
BslFI GGGAC 5 cut(s) 46, 91, 605, 828, 1256
BslI CCNNNNNNNGG 6 cut(s) 96, 167, 1880, 1967, 2311, 2447
BsmAI GTCTC 6 cut(s) 1030, 1725, 2000, 2259, 3034, 3262
BsmBI CGTCTC 1 cut(s) 3034
BsmFI GGGAC 5 cut(s) 46, 91, 605, 828, 1256
BsnI GGCC 8 cut(s) 100, 144, 171, 227, 305, 1801, 2514, 2592
Bso31I GGTCTC 2 cut(s) 2000, 3262
Bsp1286I GDGCHC 7 cut(s) 416, 837, 1477, 1519, 3045, 3090, 3570
Bsp13I TCCGGA 1 cut(s) 1738
Bsp1720I GCTNAGC 1 cut(s) 1446
Bsp19I CCATGG 2 cut(s) 858, 2966
BspANI GGCC 8 cut(s) 100, 144, 171, 227, 305, 1801, 2514, 2592
BspCNI CTCAG 9 cut(s) 1040, 1075, 1078, 1141, 1399, 1438, 1726, 1996, 2290
BspDI ATCGAT 1 cut(s) 453
BspEI TCCGGA 1 cut(s) 1738
BspFNI CGCG 2 cut(s) 256, 1249
BspHI TCATGA 4 cut(s) 129, 1342, 2535, 3123
BspLI GGNNCC 8 cut(s) 320, 834, 1898, 1921, 2331, 2949, 3089, 3346
BspMAI CTGCAG 3 cut(s) 1177, 2253, 2322
BspPI GGATC 7 cut(s) 1477, 1863, 1880, 2483, 2522, 2565, 3194
BspQI GCTCTTC 2 cut(s) 1637, 2140
BspT107I GGYRCC 4 cut(s) 318, 2947, 3087, 3344
BspTNI GGTCTC 2 cut(s) 2000, 3262
BsrBI CCGCTC 1 cut(s) 27
BsrDI GCAATG 1 cut(s) 3407
BsrFI RCCGGY 4 cut(s) 167, 191, 310, 3023
BsrI ACTGG 9 cut(s) 436, 564, 1552, 1831, 2010, 3084, 3258, 3305, 3469
BssAI RCCGGY 4 cut(s) 167, 191, 310, 3023
BssECI CCNNGG 5 cut(s) 118, 858, 1706, 2966, 3069
BssNI GRCGYC 3 cut(s) 213, 2243, 3027
BssT1I CCWWGG 3 cut(s) 858, 1706, 2966
Bst2UI CCWGG 8 cut(s) 224, 362, 1494, 1803, 2110, 2850, 3070, 3342
Bst4CI ACNGT 5 cut(s) 1663, 1703, 2404, 2483, 3484
Bst6I CTCTTC 5 cut(s) 1637, 2134, 2140, 2730, 3629
BstACI GRCGYC 3 cut(s) 213, 2243, 3027
BstAPI GCANNNNNTGC 1 cut(s) 3409
BstDSI CCRYGG 2 cut(s) 858, 2966
BstENI CCTNNNNNAGG 1 cut(s) 2309
BstF5I GGATG 9 cut(s) 128, 576, 655, 1675, 1840, 1870, 2105, 2761, 3088
BstFNI CGCG 2 cut(s) 256, 1249
BstH2I RGCGCY 1 cut(s) 3678
BstHHI GCGC 1 cut(s) 3677
BstMAI GTCTC 6 cut(s) 1030, 1725, 2000, 2259, 3034, 3262
BstMCI CGRYCG 2 cut(s) 101, 306
BstMWI GCNNNNNNNGC 9 cut(s) 311, 334, 832, 901, 1269, 1679, 2570, 3409, 3560
BstNI CCWGG 8 cut(s) 224, 362, 1494, 1803, 2110, 2850, 3070, 3342
BstNSI RCATGY 3 cut(s) 767, 1325, 1772
BstSFI CTRYAG 4 cut(s) 1111, 1173, 2249, 2318
BstSLI GKGCMC 1 cut(s) 3090
BstUI CGCG 2 cut(s) 256, 1249
BstV2I GAAGAC 1 cut(s) 3270
BstX2I RGATCY 6 cut(s) 1885, 2395, 2488, 2527, 2557, 3199
BstXI CCANNNNNNTGG 2 cut(s) 1493, 2967
BstYI RGATCY 6 cut(s) 1885, 2395, 2488, 2527, 2557, 3199
BstZI CGGCCG 2 cut(s) 98, 303
Bsu15I ATCGAT 1 cut(s) 453
BsuI GTATCC 1 cut(s) 3101
BsuRI GGCC 8 cut(s) 100, 144, 171, 227, 305, 1801, 2514, 2592
BsuTUI ATCGAT 1 cut(s) 453
BtgI CCRYGG 2 cut(s) 858, 2966
BtrI CACGTC 1 cut(s) 1602
BtsCI GGATG 9 cut(s) 128, 576, 655, 1675, 1840, 1870, 2105, 2761, 3088
BtsI GCAGTG 1 cut(s) 2315
BtsIMutI CAGTG 2 cut(s) 2315, 3489
CciI TCATGA 4 cut(s) 129, 1342, 2535, 3123
CfoI GCGC 1 cut(s) 3677
Cfr10I RCCGGY 4 cut(s) 167, 191, 310, 3023
Cfr13I GGNCC 5 cut(s) 388, 1510, 1897, 3338, 3477
ClaI ATCGAT 1 cut(s) 453
CpoI CGGWCCG 2 cut(s) 388, 1510
CseI GACGC 2 cut(s) 2251, 3016
CsiI ACCWGGT 1 cut(s) 1492
Csp6I GTAC 9 cut(s) 319, 358, 1069, 1125, 1462, 1699, 2674, 2915, 3308
CspCI CAANNNNNGTGG 2 cut(s) 3589, 3624
CspI CGGWCCG 2 cut(s) 388, 1510
CviQI GTAC 9 cut(s) 319, 358, 1069, 1125, 1462, 1699, 2674, 2915, 3308
DrdI GACNNNNNNGTC 1 cut(s) 1717
DriI GACNNNNNGTC 1 cut(s) 3616
DseDI GACNNNNNNGTC 1 cut(s) 1717
EaeI YGGCCR 4 cut(s) 98, 142, 303, 2590
EagI CGGCCG 2 cut(s) 98, 303
Eam1104I CTCTTC 5 cut(s) 1637, 2134, 2140, 2730, 3629
Eam1105I GACNNNNNGTC 1 cut(s) 3616
EarI CTCTTC 5 cut(s) 1637, 2134, 2140, 2730, 3629
EciI GGCGGA 3 cut(s) 178, 278, 304
Ecl136II GAGCTC 1 cut(s) 414
EclXI CGGCCG 2 cut(s) 98, 303
Eco130I CCWWGG 3 cut(s) 858, 1706, 2966
Eco147I AGGCCT 2 cut(s) 1801, 2514
Eco24I GRGCYC 2 cut(s) 416, 837
Eco31I GGTCTC 2 cut(s) 2000, 3262
Eco32I GATATC 2 cut(s) 1553, 2115
Eco47I GGWCC 5 cut(s) 388, 1510, 1897, 3338, 3477
Eco47III AGCGCT 1 cut(s) 3676
Eco52I CGGCCG 2 cut(s) 98, 303
Eco53kI GAGCTC 1 cut(s) 414
Eco57I CTGAAG 5 cut(s) 857, 2384, 3113, 3282, 3579
EcoICRI GAGCTC 1 cut(s) 414
EcoNI CCTNNNNNAGG 1 cut(s) 2309
EcoO109I RGGNCCY 1 cut(s) 1897
EcoRI GAATTC 4 cut(s) 184, 867, 2929, 2998
EcoRII CCWGG 8 cut(s) 222, 360, 1492, 1801, 2108, 2848, 3068, 3340
EcoRV GATATC 2 cut(s) 1553, 2115
EcoT14I CCWWGG 3 cut(s) 858, 1706, 2966
EcoT38I GRGCYC 2 cut(s) 416, 837
ErhI CCWWGG 3 cut(s) 858, 1706, 2966
Esp3I CGTCTC 1 cut(s) 3034
FalI AAGNNNNNCTT 8 cut(s) 461, 493, 711, 743, 1353, 1385, 2723, 2755
FaqI GGGAC 5 cut(s) 46, 91, 605, 828, 1256
FauI CCCGC 3 cut(s) 80, 630, 3368
FauNDI CATATG 3 cut(s) 2071, 2629, 3615
FbaI TGATCA 2 cut(s) 544, 2770
FblI GTMKAC 2 cut(s) 1296, 2278
FokI GGATG 9 cut(s) 135, 583, 662, 1682, 1847, 1857, 2092, 2768, 3095
FriOI GRGCYC 2 cut(s) 416, 837
FspBI CTAG 6 cut(s) 375, 1134, 1538, 1890, 1929, 2972
GlaI GCGC 1 cut(s) 3676
GsuI CTGGAG 2 cut(s) 1547, 2701
HaeII RGCGCY 1 cut(s) 3678
HaeIII GGCC 8 cut(s) 100, 144, 171, 227, 305, 1801, 2514, 2592
HgaI GACGC 2 cut(s) 2251, 3016
HhaI GCGC 1 cut(s) 3677
Hin1I GRCGYC 3 cut(s) 213, 2243, 3027
Hin6I GCGC 1 cut(s) 3675
HinP1I GCGC 1 cut(s) 3675
HincII GTYRAC 2 cut(s) 474, 1297
HindII GTYRAC 2 cut(s) 474, 1297
HindIII AAGCTT 3 cut(s) 883, 1626, 3191
HpaI GTTAAC 1 cut(s) 474
HphI GGTGA 5 cut(s) 149, 157, 1043, 2316, 3001
Hpy166II GTNNAC 7 cut(s) 474, 1297, 2264, 2279, 2869, 3077, 3480
Hpy8I GTNNAC 7 cut(s) 474, 1297, 2264, 2279, 2869, 3077, 3480
Hpy99I CGWCG 5 cut(s) 244, 247, 250, 253, 256
HpyAV CCTTC 9 cut(s) 25, 520, 923, 1220, 1846, 2548, 3020, 3528, 3538
HpyCH4III ACNGT 5 cut(s) 1663, 1703, 2404, 2483, 3484
HpyCH4IV ACGT 3 cut(s) 213, 1348, 1601
HpyF10VI GCNNNNNNNGC 9 cut(s) 311, 334, 832, 901, 1269, 1679, 2570, 3409, 3560
HpySE526I ACGT 3 cut(s) 213, 1348, 1601
Hsp92I GRCGYC 3 cut(s) 213, 2243, 3027
HspAI GCGC 1 cut(s) 3675
Kpn2I TCCGGA 1 cut(s) 1738
KpnI GGTACC 1 cut(s) 322
KroI GCCGGC 2 cut(s) 191, 310
KroNI GCCGGC 2 cut(s) 193, 312
Ksp22I TGATCA 2 cut(s) 544, 2770
KspAI GTTAAC 1 cut(s) 474
LguI GCTCTTC 2 cut(s) 1637, 2140
LmnI GCTCC 5 cut(s) 294, 832, 1480, 3048, 3439
LweI GCATC 9 cut(s) 113, 697, 930, 1660, 2065, 2194, 2529, 2746, 3291
MabI ACCWGGT 1 cut(s) 1492
MaeI CTAG 6 cut(s) 375, 1134, 1538, 1890, 1929, 2972
MaeII ACGT 3 cut(s) 213, 1348, 1601
MaeIII GTNAC 8 cut(s) 163, 203, 214, 1208, 1315, 2174, 2322, 3380
MbiI CCGCTC 1 cut(s) 27
MfeI CAATTG 1 cut(s) 2222
MflI RGATCY 6 cut(s) 1885, 2395, 2488, 2527, 2557, 3199
MhlI GDGCHC 7 cut(s) 416, 837, 1477, 1519, 3045, 3090, 3570
MlyI GAGTC 7 cut(s) 377, 810, 1705, 1727, 1736, 2284, 2714
MmeI TCCRAC 2 cut(s) 1712, 2618
MroI TCCGGA 1 cut(s) 1738
MroNI GCCGGC 2 cut(s) 191, 310
MroXI GAANNNNTTC 5 cut(s) 1365, 2293, 2735, 2857, 3180
MseI TTAA 8 cut(s) 473, 992, 1439, 1467, 1559, 1755, 3158, 3575
MslI CAYNNNNRTG 4 cut(s) 56, 699, 1347, 2965
MspA1I CMGCKG 2 cut(s) 286, 3400
MunI CAATTG 1 cut(s) 2222
MvaI CCWGG 8 cut(s) 224, 362, 1494, 1803, 2110, 2850, 3070, 3342
MvnI CGCG 2 cut(s) 256, 1249
MwoI GCNNNNNNNGC 9 cut(s) 311, 334, 832, 901, 1269, 1679, 2570, 3409, 3560
NaeI GCCGGC 2 cut(s) 193, 312
NciI CCSGG 5 cut(s) 392, 422, 1508, 2131, 2166
NcoI CCATGG 2 cut(s) 858, 2966
NdeI CATATG 3 cut(s) 2071, 2629, 3615
NgoMIV GCCGGC 2 cut(s) 191, 310
NlaIV GGNNCC 8 cut(s) 320, 834, 1898, 1921, 2331, 2949, 3089, 3346
NmuCI GTSAC 4 cut(s) 163, 203, 214, 2322
NspI RCATGY 3 cut(s) 767, 1325, 1772
PaeI GCATGC 1 cut(s) 767
PagI TCATGA 4 cut(s) 129, 1342, 2535, 3123
PceI AGGCCT 2 cut(s) 1801, 2514
PciI ACATGT 1 cut(s) 1321
PciSI GCTCTTC 2 cut(s) 1637, 2140
PcsI WCGNNNNNNNCGW 3 cut(s) 236, 245, 248
PdiI GCCGGC 2 cut(s) 193, 312
PdmI GAANNNNTTC 5 cut(s) 1365, 2293, 2735, 2857, 3180
PflMI CCANNNNNTGG 2 cut(s) 1967, 2447
PfoI TCCNGGA 2 cut(s) 2108, 2848
PleI GAGTC 7 cut(s) 376, 810, 1705, 1726, 1736, 2283, 2714
PpsI GAGTC 7 cut(s) 376, 810, 1705, 1726, 1736, 2283, 2714
PpuMI RGGWCCY 1 cut(s) 1897
PscI ACATGT 1 cut(s) 1321
Psp124BI GAGCTC 1 cut(s) 416
Psp5II RGGWCCY 1 cut(s) 1897
Psp6I CCWGG 8 cut(s) 222, 360, 1492, 1801, 2108, 2848, 3068, 3340
PspGI CCWGG 8 cut(s) 222, 360, 1492, 1801, 2108, 2848, 3068, 3340
PspN4I GGNNCC 8 cut(s) 320, 834, 1898, 1921, 2331, 2949, 3089, 3346
PspPI GGNCC 5 cut(s) 388, 1510, 1897, 3338, 3477
PspPPI RGGWCCY 1 cut(s) 1897
PstI CTGCAG 3 cut(s) 1177, 2253, 2322
PsuI RGATCY 6 cut(s) 1885, 2395, 2488, 2527, 2557, 3199
PvuII CAGCTG 1 cut(s) 3400
RsaI GTAC 9 cut(s) 320, 359, 1070, 1126, 1463, 1700, 2675, 2916, 3309
RsaNI GTAC 9 cut(s) 319, 358, 1069, 1125, 1462, 1699, 2674, 2915, 3308
RseI CAYNNNNRTG 4 cut(s) 56, 699, 1347, 2965
Rsr2I CGGWCCG 2 cut(s) 388, 1510
RsrII CGGWCCG 2 cut(s) 388, 1510
SacI GAGCTC 1 cut(s) 416
SalI GTCGAC 1 cut(s) 1295
SapI GCTCTTC 2 cut(s) 1637, 2140
SaqAI TTAA 8 cut(s) 473, 992, 1439, 1467, 1559, 1755, 3158, 3575
Sau96I GGNCC 5 cut(s) 388, 1510, 1897, 3338, 3477
ScaI AGTACT 2 cut(s) 2675, 2916
SchI GAGTC 7 cut(s) 377, 810, 1705, 1727, 1736, 2284, 2714
SduI GDGCHC 7 cut(s) 416, 837, 1477, 1519, 3045, 3090, 3570
SexAI ACCWGGT 1 cut(s) 1492
SfaNI GCATC 9 cut(s) 113, 697, 930, 1660, 2065, 2194, 2529, 2746, 3291
SfcI CTRYAG 4 cut(s) 1111, 1173, 2249, 2318
SinI GGWCC 5 cut(s) 388, 1510, 1897, 3338, 3477
SmiMI CAYNNNNRTG 4 cut(s) 56, 699, 1347, 2965
SmlI CTYRAG 4 cut(s) 1623, 2750, 3170, 3349
SmoI CTYRAG 4 cut(s) 1623, 2750, 3170, 3349
SphI GCATGC 1 cut(s) 767
SseBI AGGCCT 2 cut(s) 1801, 2514
SspI AATATT 1 cut(s) 1534
SspMI CTAG 6 cut(s) 375, 1134, 1538, 1890, 1929, 2972
SstI GAGCTC 1 cut(s) 416
StuI AGGCCT 2 cut(s) 1801, 2514
StyI CCWWGG 3 cut(s) 858, 1706, 2966
TaaI ACNGT 5 cut(s) 1663, 1703, 2404, 2483, 3484
TaiI ACGT 3 cut(s) 216, 1351, 1604
TaqI TCGA 7 cut(s) 437, 453, 515, 927, 1296, 2882, 2927
TaqII GACCGA 1 cut(s) 3465
TatI WGTACW 5 cut(s) 1068, 1124, 1698, 2673, 2914
TauI GCSGC 6 cut(s) 103, 263, 289, 305, 1252, 3554
Tru1I TTAA 8 cut(s) 473, 992, 1439, 1467, 1559, 1755, 3158, 3575
Tru9I TTAA 8 cut(s) 473, 992, 1439, 1467, 1559, 1755, 3158, 3575
TscAI CASTG 2 cut(s) 2322, 3489
TseFI GTSAC 4 cut(s) 163, 203, 214, 2322
Tsp45I GTSAC 4 cut(s) 163, 203, 214, 2322
TspGWI ACGGA 1 cut(s) 287
TspRI CASTG 2 cut(s) 2322, 3489
Van91I CCANNNNNTGG 2 cut(s) 1967, 2447
VpaK11BI GGWCC 5 cut(s) 388, 1510, 1897, 3338, 3477
XagI CCTNNNNNAGG 1 cut(s) 2309
XapI RAATTY 7 cut(s) 184, 754, 867, 2686, 2929, 2955, 2998
XbaI TCTAGA 1 cut(s) 1537
XceI RCATGY 3 cut(s) 767, 1325, 1772
XcmI CCANNNNNNNNNTGG 1 cut(s) 3076
XmiI GTMKAC 2 cut(s) 1296, 2278
XmnI GAANNNNTTC 5 cut(s) 1365, 2293, 2735, 2857, 3180
XspI CTAG 6 cut(s) 375, 1134, 1538, 1890, 1929, 2972
ZraI GACGTC 1 cut(s) 214
ZrmI AGTACT 2 cut(s) 2675, 2916
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.