AT1G61030

Wings apart-like protein regulation of heterochromatin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0009354
Physical Location & Seq
Forward (+)
22477896 .. 22482451
4556 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G61030.1

Sequence Viewer

Length: 2523 bp
ATGATGGAGCGAACTTACGGCCGTCGCAAGCCGGGGATGTTAAACGACGACGTCTCGCGGGCGGAACATATTTTTCCGTCTTCTTCATCTCCAGAACTCGAGCCAGTTGATTTCTCAACTCAAGAATCATCTTGTGTCTGGAATTATTCTTCAAGATCCACTTTTTCGGATAATGATTTTTCTGAGAAGAGGAACAAGAGACCCAGAAACGGTGGTGGAGGGTTTGGTTCGAATTCGACTTTGATGGAGGCTCAAGAGTTCGGCGAGTTAATTGAGAATGAGGACGAAGTTAACTTCGCTCTTGATGGGTTGAAGAAAGGACATAAAGTTAGGATCAGAAGAGCTGCTTTGTCCTCTCTACTCTCGATTTGTGAATCTCAGTATCAGCGACGCTCTTTGAGAGCTTTAGGGATCTCACAGTCCATAATTGATGCGATTTTGGGTCTTTGCCTTGATGACATACCAAGCAATCTTGCTGCAGCTACCCTTTTCTTTGTGCTAACTACTGATGGTCAAGATGATCACTTTATGGAGTCACCCAACTCCATCAAGTTTTTGGTTAAGCTGTTGAGACCTGTGGTTTCTGCCAGTACTAAAGTAAAGCCGCGGAATATAGGATCTAGGCTCTTATCAATAATCAAGGATGTTGATGCAGCACGTGATGCAGCCAGTATGCATGATTTAAGCTCCTGTGATATAATTGACAGAGCTCAGGAAATTCTTGTTAATTGCAAGGAGTTGAGATTGATTGATAGCTATAAAATCGAGAGGATGAGGCCTGAGCTAAGTACGAAATGGGTAGCTTTGTTGGTGATGGAGAAGGCGTGCTTGTCCAAAATCTCTTTTGATGATACCTCTGGTACTGTGAAAAAATCTGGAGGAATGTTCAAGGAGAAACTGAGAGAACTTGGAGGACTTGATGCTGTCTTTGATGTTGTTATGGATTGCCACACTGTAATGGAGAGCTGGGTGACTCATGACACACTCTCTGTCGAGGATATAAAAGATGACTTGAATAAGCAGAGTCTGATGTTACTTCTGAAATGTCTAAAAATCATGGAGAATGCTACATTCCTCAGCACAGAAAACCAGATTCATTTGCTCAGACTTAATAAAAGCATGGGTTCTCATGAATCCCGACTGTCTTTCACAGAGCTCATGATAAGTGTCATCAAAATACTTTCAGGTCTTCAGTTACGTGCTCACAGGAACGAAAAGCATCCTCACCCTCAGCCACATCTAGCTTCTGCTGTTAAGAAGGGCTTTGTGACAATTATTAGTTCAGATACTTGCTCTACCACTGGCTTTAGCTCCATAAAAAGTTTGAGCGTATCCAAGAGAAATCAGTCTGCATTCCTGGTAGGTTGCTCAACGACACCAAAGCCGGGGTCTCAATCAAGTGTAATGTCGACAATTGATCACTGCACGCTGACGACTACAGCTGGTTCCAACACTGGGTCGTTTGCAGGCAGATTAGCCTCATTGGGTAGTGGCATTTCCAGAAGTAAAACAAGGACTAGCCAAACTAGAGAATCCAGTTGTAAAAAAGTTGAAAACTTTGCATCCTTCGAGGATAGTCAAGATCCTTTTTCATTTGATTTAGAAGATTCAGGACCTTCCAGATGGGCAGTAGGAAAGCAAAAGAAATCTAAAGGTCAAAAGAGGAAGGGAAGCTACAGAGATAAGAAAGATGAACGCTCGCTTCAGCTCTTCTCGAGCCAGGAGGAATCAAACCATGGTTTAAATTCTCAGGAGGAATCAAGTGATAGAGATCACCATGTAACAGAGCAACCTTCTTTAACATATGACATTGATAAAGGATGTCTCTGTCTTTTATCTGATTGCCTTTTAACAGCCGTGAAGGTATTAATGAACCTAACAAATGGTAATTCTGTTGGTTGTCGGGAAGTTGCTGCCTGCGGAGGACTGGAGAGCATGGCTGAATTAGTTGTTGGACACTTTCCTTCCTTCACCAGATCTCCGCTTTACAGTCAGATGGAGTCCGGGACATGCCACCAGAAAGATAAACATCTCACAGACCAGGAATTAGATTTTCTTGTTGCCATCCTGGGGCTACTGGTAAACTTGGTGGAGAAAAACGGAATAAACAGGTCAAGGCTCGCGGCAGCTAGTGTTCCGATTACCAATCCAGAAGGGTTACAAGATAGTGAACAAGACATGATCCCTCTTTTATGTTCAATCTTTCTCACCAATAAAGGATCCGCAGACACCAAAGATGAAACGAGTACTTTCACTTTGGACGATGAAGAAGCTGTTTTAGAAAGCGAAAAGGAAGCGGAAAAGATGATCGTGGAGGCATATTCTGCCCTTCTACTTGCATTTCTATCCACAGAAAGTAGAAGTATACGAAATGCCATCAGAGATTATCTCCCAAAACGCGACATGGCAATTCTGGTACCCGTGTTGGATAGATTTGTGGCGTTTCATACAACACTGGACATGATTCCTCCAGAAACACATAAAGTGGTTATGGAAGTTATCGAGTCGTGCAAATTGCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WAPL

Protein Analysis

840

Amino Acids

92.93

Weight (kDa)

6.02

Isoelectric Point (pI)

53.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WAPL PF07814 79 - 700 5.4e-54 Wings apart-like protein regulation of heterochromatin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 54
Acc65I GGTACC 1 cut(s) 2416
AccB1I GGYRCC 1 cut(s) 2416
AccI GTMKAC 2 cut(s) 1409, 2365
AccII CGCG 4 cut(s) 58, 607, 2123, 2400
AciI CCGC 9 cut(s) 58, 62, 605, 607, 1920, 1982, 2123, 2223, 2297
AclWI GGATC 8 cut(s) 150, 341, 419, 625, 1577, 2176, 2214, 2227
AcoI YGGCCR 1 cut(s) 19
AcsI RAATTY 3 cut(s) 232, 717, 1744
AcuI CTGAAG 2 cut(s) 1175, 1688
AcvI CACGTG 1 cut(s) 659
AcyI GRCGYC 1 cut(s) 51
AfaI GTAC 5 cut(s) 592, 790, 862, 2248, 2418
AfiI CCNNNNNNNGG 4 cut(s) 209, 1385, 1455, 2070
AgsI TTSAA 6 cut(s) 153, 313, 889, 1015, 1553, 2199
AjnI CCWGG 4 cut(s) 1356, 1719, 2040, 2067
AjuI GAANNNNNNNTTGG 2 cut(s) 1935, 1967
Alw21I GWGCWC 3 cut(s) 712, 1158, 1204
Alw26I GTCTC 5 cut(s) 58, 193, 565, 1395, 1829
AlwI GGATC 8 cut(s) 150, 341, 419, 625, 1577, 2176, 2214, 2227
AlwNI CAGNNNCTG 1 cut(s) 1027
Ama87I CYCGRG 2 cut(s) 98, 1714
AoxI GGCC 2 cut(s) 19, 776
ApeKI GCWGC 7 cut(s) 344, 476, 479, 653, 665, 1913, 2126
ApoI RAATTY 3 cut(s) 232, 717, 1744
ArsI GACNNNNNNTTYG 2 cut(s) 1373, 1405
AseI ATTAAT 1 cut(s) 1868
Asp718I GGTACC 1 cut(s) 2416
AspS9I GGNCC 1 cut(s) 1613
AsuC2I CCSGG 3 cut(s) 33, 1386, 2005
AsuHPI GGTGA 7 cut(s) 528, 823, 982, 1217, 1766, 1963, 2200
AsuII TTCGAA 1 cut(s) 230
AvaI CYCGRG 2 cut(s) 98, 1714
AvaII GGWCC 1 cut(s) 1613
BaeI ACNNNNGTAYC 2 cut(s) 2408, 2441
BamHI GGATCC 1 cut(s) 2219
BanI GGYRCC 1 cut(s) 2416
BanII GRGCYC 2 cut(s) 712, 1158
BarI GAAGNNNNNNTAC 2 cut(s) 1658, 1690
BbrPI CACGTG 1 cut(s) 659
BbsI GAAGAC 2 cut(s) 72, 1181
Bbv12I GWGCWC 3 cut(s) 712, 1158, 1204
BbvCI CCTCAGC 2 cut(s) 1076, 1230
BbvI GCAGC 7 cut(s) 331, 463, 491, 665, 677, 1900, 2138
BccI CCATC 9 cut(s) 238, 299, 503, 554, 808, 1617, 1990, 2072, 2384
BceAI ACGGC 3 cut(s) 6, 34, 1841
BciT130I CCWGG 4 cut(s) 1358, 1721, 2042, 2069
BciVI GTATCC 1 cut(s) 1342
BclI TGATCA 2 cut(s) 520, 1417
BcnI CCSGG 3 cut(s) 33, 1386, 2005
BcoDI GTCTC 5 cut(s) 58, 193, 565, 1395, 1829
BfaI CTAG 5 cut(s) 621, 1241, 1518, 1527, 2130
BfmI CTRYAG 3 cut(s) 477, 1437, 1675
BfuI GTATCC 1 cut(s) 1342
BglII AGATCT 1 cut(s) 1976
BisI GCNGC 9 cut(s) 345, 477, 480, 605, 654, 666, 1914, 2124, 2127
BlsI GCNGC 9 cut(s) 346, 478, 481, 606, 655, 667, 1915, 2125, 2128
BmcAI AGTACT 2 cut(s) 592, 2248
Bme1390I CCNGG 7 cut(s) 33, 1358, 1386, 1721, 2005, 2042, 2069
Bme18I GGWCC 1 cut(s) 1613
BmeT110I CYCGRG 2 cut(s) 98, 1714
BmgT120I GGNCC 1 cut(s) 1613
BmiI GGNNCC 3 cut(s) 1447, 2221, 2418
BmrFI CCNGG 7 cut(s) 33, 1358, 1386, 1721, 2005, 2042, 2069
BmrI ACTGGG 1 cut(s) 1464
BmsI GCATC 6 cut(s) 421, 640, 652, 910, 1228, 1571
BmuI ACTGGG 1 cut(s) 1464
BpiI GAAGAC 2 cut(s) 72, 1181
BplI GAGNNNNNCTC 2 cut(s) 2373, 2405
BpmI CTGGAG 4 cut(s) 75, 897, 1949, 2454
Bpu10I CCTNAGC 4 cut(s) 711, 780, 1076, 1230
Bpu14I TTCGAA 1 cut(s) 230
BpuEI CTTGAG 2 cut(s) 105, 237
BpuMI CCSGG 3 cut(s) 33, 1386, 2005
BsaAI YACGTR 2 cut(s) 659, 1199
BsaBI GATNNNNATC 2 cut(s) 1770, 2028
BsaHI GRCGYC 1 cut(s) 51
BsaI GGTCTC 3 cut(s) 193, 565, 1395
BsaJI CCNNGG 5 cut(s) 32, 605, 1385, 1735, 2068
BsaXI ACNNNNNCTCC 4 cut(s) 809, 839, 1963, 1993
Bsc4I CCNNNNNNNGG 4 cut(s) 209, 1385, 1455, 2070
Bse1I ACTGG 9 cut(s) 104, 588, 669, 1306, 1459, 1536, 1932, 2082, 2460
Bse8I GATNNNNATC 2 cut(s) 1770, 2028
BseBI CCWGG 4 cut(s) 1358, 1721, 2042, 2069
BseDI CCNNGG 5 cut(s) 32, 605, 1385, 1735, 2068
BseGI GGATG 7 cut(s) 42, 649, 777, 1219, 1562, 1826, 2064
BseJI GATNNNNATC 2 cut(s) 1770, 2028
BseLI CCNNNNNNNGG 4 cut(s) 209, 1385, 1455, 2070
BseMII CTCAG 9 cut(s) 174, 392, 725, 771, 890, 1090, 1117, 1244, 1763
BseNI ACTGG 9 cut(s) 104, 588, 669, 1306, 1459, 1536, 1932, 2082, 2460
BseX3I CGGCCG 1 cut(s) 19
BseXI GCAGC 7 cut(s) 331, 463, 491, 665, 677, 1900, 2138
BseYI CCCAGC 1 cut(s) 966
BsgI GTGCAG 1 cut(s) 1408
Bsh1236I CGCG 4 cut(s) 58, 607, 2123, 2400
Bsh1285I CGRYCG 1 cut(s) 22
BshFI GGCC 2 cut(s) 21, 778
BshNI GGYRCC 1 cut(s) 2416
BsiEI CGRYCG 1 cut(s) 22
BsiHKAI GWGCWC 3 cut(s) 712, 1158, 1204
BsiHKCI CYCGRG 2 cut(s) 98, 1714
BsiSI CCGG 3 cut(s) 32, 1385, 2004
BslFI GGGAC 1 cut(s) 2020
BslI CCNNNNNNNGG 4 cut(s) 209, 1385, 1455, 2070
BsmAI GTCTC 5 cut(s) 58, 193, 565, 1395, 1829
BsmBI CGTCTC 1 cut(s) 58
BsmFI GGGAC 1 cut(s) 2020
BsmI GAATGC 2 cut(s) 1069, 1352
BsnI GGCC 2 cut(s) 21, 778
Bso31I GGTCTC 3 cut(s) 193, 565, 1395
BsoBI CYCGRG 2 cut(s) 98, 1714
Bsp119I TTCGAA 1 cut(s) 230
Bsp1286I GDGCHC 3 cut(s) 712, 1158, 1204
Bsp19I CCATGG 1 cut(s) 1735
BspACI CCGC 9 cut(s) 58, 62, 605, 607, 1920, 1982, 2123, 2223, 2297
BspANI GGCC 2 cut(s) 21, 778
BspCNI CTCAG 9 cut(s) 175, 391, 724, 772, 891, 1089, 1116, 1243, 1762
BspFNI CGCG 4 cut(s) 58, 607, 2123, 2400
BspHI TCATGA 3 cut(s) 976, 1129, 1158
BspLI GGNNCC 3 cut(s) 1447, 2221, 2418
BspMAI CTGCAG 1 cut(s) 481
BspPI GGATC 8 cut(s) 150, 341, 419, 625, 1577, 2176, 2214, 2227
BspQI GCTCTTC 2 cut(s) 334, 1715
BspT104I TTCGAA 1 cut(s) 230
BspT107I GGYRCC 1 cut(s) 2416
BspTNI GGTCTC 3 cut(s) 193, 565, 1395
BsrI ACTGG 9 cut(s) 104, 588, 669, 1306, 1459, 1536, 1932, 2082, 2460
BssECI CCNNGG 5 cut(s) 32, 605, 1385, 1735, 2068
BssNAI GTATAC 1 cut(s) 2366
BssNI GRCGYC 1 cut(s) 51
BssT1I CCWWGG 1 cut(s) 1735
Bst1107I GTATAC 1 cut(s) 2366
Bst2UI CCWGG 4 cut(s) 1358, 1721, 2042, 2069
Bst4CI ACNGT 6 cut(s) 212, 420, 865, 955, 1143, 1991
Bst6I CTCTTC 3 cut(s) 182, 334, 1715
BstACI GRCGYC 1 cut(s) 51
BstAPI GCANNNNNTGC 2 cut(s) 662, 2324
BstBAI YACGTR 2 cut(s) 659, 1199
BstBI TTCGAA 1 cut(s) 230
BstC8I GCNNGC 8 cut(s) 29, 60, 826, 1427, 1468, 1700, 1918, 2121
BstDSI CCRYGG 2 cut(s) 605, 1735
BstF5I GGATG 7 cut(s) 42, 649, 777, 1219, 1562, 1826, 2064
BstFNI CGCG 4 cut(s) 58, 607, 2123, 2400
BstMAI GTCTC 5 cut(s) 58, 193, 565, 1395, 1829
BstMCI CGRYCG 1 cut(s) 22
BstMWI GCNNNNNNNGC 2 cut(s) 662, 2324
BstNI CCWGG 4 cut(s) 1358, 1721, 2042, 2069
BstNSI RCATGY 1 cut(s) 2013
BstSCI CCNGG 7 cut(s) 31, 1356, 1384, 1719, 2003, 2040, 2067
BstSFI CTRYAG 3 cut(s) 477, 1437, 1675
BstUI CGCG 4 cut(s) 58, 607, 2123, 2400
BstV1I GCAGC 7 cut(s) 331, 463, 491, 665, 677, 1900, 2138
BstV2I GAAGAC 2 cut(s) 72, 1181
BstX2I RGATCY 6 cut(s) 155, 411, 617, 1582, 1976, 2219
BstYI RGATCY 6 cut(s) 155, 411, 617, 1582, 1976, 2219
BstZ17I GTATAC 1 cut(s) 2366
BstZI CGGCCG 1 cut(s) 19
BsuI GTATCC 1 cut(s) 1342
BsuRI GGCC 2 cut(s) 21, 778
BtgI CCRYGG 2 cut(s) 605, 1735
BtsCI GGATG 7 cut(s) 42, 649, 777, 1219, 1562, 1826, 2064
BtsI GCAGTG 1 cut(s) 1420
BtsIMutI CAGTG 5 cut(s) 951, 1299, 1420, 1452, 2453
Cac8I GCNNGC 8 cut(s) 29, 60, 826, 1427, 1468, 1700, 1918, 2121
CaiI CAGNNNCTG 1 cut(s) 1027
CciI TCATGA 3 cut(s) 976, 1129, 1158
Cfr13I GGNCC 1 cut(s) 1613
Cfr42I CCGCGG 1 cut(s) 608
CseI GACGC 1 cut(s) 399
Csp6I GTAC 5 cut(s) 591, 789, 861, 2247, 2417
CviQI GTAC 5 cut(s) 591, 789, 861, 2247, 2417
DraI TTTAAA 1 cut(s) 1743
EaeI YGGCCR 1 cut(s) 19
EagI CGGCCG 1 cut(s) 19
Eam1104I CTCTTC 3 cut(s) 182, 334, 1715
EarI CTCTTC 3 cut(s) 182, 334, 1715
EciI GGCGGA 1 cut(s) 77
Ecl136II GAGCTC 2 cut(s) 710, 1156
EclXI CGGCCG 1 cut(s) 19
Eco130I CCWWGG 1 cut(s) 1735
Eco147I AGGCCT 1 cut(s) 778
Eco24I GRGCYC 2 cut(s) 712, 1158
Eco31I GGTCTC 3 cut(s) 193, 565, 1395
Eco47I GGWCC 1 cut(s) 1613
Eco52I CGGCCG 1 cut(s) 19
Eco53kI GAGCTC 2 cut(s) 710, 1156
Eco57I CTGAAG 2 cut(s) 1175, 1688
Eco72I CACGTG 1 cut(s) 659
Eco88I CYCGRG 2 cut(s) 98, 1714
EcoICRI GAGCTC 2 cut(s) 710, 1156
EcoO109I RGGNCCY 1 cut(s) 1613
EcoRI GAATTC 1 cut(s) 232
EcoRII CCWGG 4 cut(s) 1356, 1719, 2040, 2067
EcoT14I CCWWGG 1 cut(s) 1735
EcoT22I ATGCAT 1 cut(s) 678
EcoT38I GRGCYC 2 cut(s) 712, 1158
ErhI CCWWGG 1 cut(s) 1735
Esp3I CGTCTC 1 cut(s) 58
FalI AAGNNNNNCTT 8 cut(s) 145, 177, 331, 363, 812, 844, 1247, 1279
FaqI GGGAC 1 cut(s) 2020
FauI CCCGC 1 cut(s) 51
FauNDI CATATG 1 cut(s) 1804
FbaI TGATCA 2 cut(s) 520, 1417
FblI GTMKAC 2 cut(s) 1409, 2365
Fnu4HI GCNGC 9 cut(s) 345, 477, 480, 605, 654, 666, 1914, 2124, 2127
FokI GGATG 7 cut(s) 49, 656, 784, 1206, 1549, 1833, 2051
FriOI GRGCYC 2 cut(s) 712, 1158
Fsp4HI GCNGC 9 cut(s) 345, 477, 480, 605, 654, 666, 1914, 2124, 2127
FspBI CTAG 5 cut(s) 621, 1241, 1518, 1527, 2130
GluI GCNGC 9 cut(s) 345, 477, 480, 605, 654, 666, 1914, 2124, 2127
GsaI CCCAGC 1 cut(s) 970
GsuI CTGGAG 4 cut(s) 75, 897, 1949, 2454
HaeIII GGCC 2 cut(s) 21, 778
HapII CCGG 3 cut(s) 32, 1385, 2004
HgaI GACGC 1 cut(s) 399
Hin1I GRCGYC 1 cut(s) 51
HincII GTYRAC 2 cut(s) 292, 1410
HindII GTYRAC 2 cut(s) 292, 1410
HpaI GTTAAC 1 cut(s) 292
HpaII CCGG 3 cut(s) 32, 1385, 2004
HphI GGTGA 7 cut(s) 528, 823, 982, 1217, 1766, 1963, 2200
Hpy166II GTNNAC 5 cut(s) 292, 1410, 2083, 2171, 2366
Hpy8I GTNNAC 5 cut(s) 292, 1410, 2083, 2171, 2366
Hpy99I CGWCG 4 cut(s) 27, 50, 53, 393
HpyCH4III ACNGT 6 cut(s) 212, 420, 865, 955, 1143, 1991
HpyCH4IV ACGT 3 cut(s) 51, 658, 1198
HpyF10VI GCNNNNNNNGC 2 cut(s) 662, 2324
HpySE526I ACGT 3 cut(s) 51, 658, 1198
Hsp92I GRCGYC 1 cut(s) 51
KpnI GGTACC 1 cut(s) 2420
Ksp22I TGATCA 2 cut(s) 520, 1417
KspAI GTTAAC 1 cut(s) 292
KspI CCGCGG 1 cut(s) 608
LguI GCTCTTC 2 cut(s) 334, 1715
LmnI GCTCC 3 cut(s) 7, 692, 1316
Lsp1109I GCAGC 7 cut(s) 331, 463, 491, 665, 677, 1900, 2138
LweI GCATC 6 cut(s) 421, 640, 652, 910, 1228, 1571
MaeI CTAG 5 cut(s) 621, 1241, 1518, 1527, 2130
MaeII ACGT 3 cut(s) 51, 658, 1198
MaeIII GTNAC 7 cut(s) 534, 970, 1032, 1194, 1267, 1780, 2157
MfeI CAATTG 1 cut(s) 1413
MflI RGATCY 6 cut(s) 155, 411, 617, 1582, 1976, 2219
MhlI GDGCHC 3 cut(s) 712, 1158, 1204
MlyI GAGTC 5 cut(s) 542, 967, 1033, 2009, 2513
MmeI TCCRAC 3 cut(s) 1473, 1933, 2406
Mph1103I ATGCAT 1 cut(s) 678
MslI CAYNNNNRTG 1 cut(s) 956
MspA1I CMGCKG 2 cut(s) 607, 1442
MspI CCGG 3 cut(s) 32, 1385, 2004
MspR9I CCNGG 7 cut(s) 33, 1358, 1386, 1721, 2005, 2042, 2069
MunI CAATTG 1 cut(s) 1413
Mva1269I GAATGC 2 cut(s) 1069, 1352
MvaI CCWGG 4 cut(s) 1358, 1721, 2042, 2069
MvnI CGCG 4 cut(s) 58, 607, 2123, 2400
MwoI GCNNNNNNNGC 2 cut(s) 662, 2324
NciI CCSGG 3 cut(s) 33, 1386, 2005
NcoI CCATGG 1 cut(s) 1735
NdeI CATATG 1 cut(s) 1804
NlaIV GGNNCC 3 cut(s) 1447, 2221, 2418
NmuCI GTSAC 3 cut(s) 534, 970, 1267
NsiI ATGCAT 1 cut(s) 678
NspI RCATGY 1 cut(s) 2013
NspV TTCGAA 1 cut(s) 230
PaeR7I CTCGAG 2 cut(s) 98, 1714
PagI TCATGA 3 cut(s) 976, 1129, 1158
PceI AGGCCT 1 cut(s) 778
PciSI GCTCTTC 2 cut(s) 334, 1715
PctI GAATGC 2 cut(s) 1069, 1352
PfeI GAWTC 9 cut(s) 125, 374, 1093, 1133, 1532, 1607, 1727, 1757, 2464
PflFI GACNNNGTC 1 cut(s) 50
PfoI TCCNGGA 1 cut(s) 2003
PkrI GCNGC 9 cut(s) 346, 478, 481, 606, 655, 667, 1915, 2125, 2128
PleI GAGTC 5 cut(s) 541, 967, 1032, 2008, 2512
PmaCI CACGTG 1 cut(s) 659
PmlI CACGTG 1 cut(s) 659
PpsI GAGTC 5 cut(s) 541, 967, 1032, 2008, 2512
Ppu21I YACGTR 2 cut(s) 659, 1199
PpuMI RGGWCCY 1 cut(s) 1613
PshBI ATTAAT 1 cut(s) 1868
Psp124BI GAGCTC 2 cut(s) 712, 1158
Psp5II RGGWCCY 1 cut(s) 1613
Psp6I CCWGG 4 cut(s) 1356, 1719, 2040, 2067
PspCI CACGTG 1 cut(s) 659
PspFI CCCAGC 1 cut(s) 966
PspGI CCWGG 4 cut(s) 1356, 1719, 2040, 2067
PspN4I GGNNCC 3 cut(s) 1447, 2221, 2418
PspPI GGNCC 1 cut(s) 1613
PspPPI RGGWCCY 1 cut(s) 1613
PspXI VCTCGAGB 1 cut(s) 98
PstI CTGCAG 1 cut(s) 481
PstNI CAGNNNCTG 1 cut(s) 1027
PsuI RGATCY 6 cut(s) 155, 411, 617, 1582, 1976, 2219
PsyI GACNNNGTC 1 cut(s) 50
PvuII CAGCTG 1 cut(s) 1442
RsaI GTAC 5 cut(s) 592, 790, 862, 2248, 2418
RsaNI GTAC 5 cut(s) 591, 789, 861, 2247, 2417
RseI CAYNNNNRTG 1 cut(s) 956
SacI GAGCTC 2 cut(s) 712, 1158
SacII CCGCGG 1 cut(s) 608
SalI GTCGAC 1 cut(s) 1408
SapI GCTCTTC 2 cut(s) 334, 1715
SatI GCNGC 9 cut(s) 345, 477, 480, 605, 654, 666, 1914, 2124, 2127
Sau96I GGNCC 1 cut(s) 1613
ScaI AGTACT 2 cut(s) 592, 2248
SchI GAGTC 5 cut(s) 542, 967, 1033, 2009, 2513
ScrFI CCNGG 7 cut(s) 33, 1358, 1386, 1721, 2005, 2042, 2069
SduI GDGCHC 3 cut(s) 712, 1158, 1204
SfaNI GCATC 6 cut(s) 421, 640, 652, 910, 1228, 1571
SfcI CTRYAG 3 cut(s) 477, 1437, 1675
Sfr274I CTCGAG 2 cut(s) 98, 1714
Sfr303I CCGCGG 1 cut(s) 608
SfuI TTCGAA 1 cut(s) 230
SgrBI CCGCGG 1 cut(s) 608
SinI GGWCC 1 cut(s) 1613
SlaI CTCGAG 2 cut(s) 98, 1714
SmiMI CAYNNNNRTG 1 cut(s) 956
SmlI CTYRAG 4 cut(s) 98, 120, 252, 1714
SmoI CTYRAG 4 cut(s) 98, 120, 252, 1714
SseBI AGGCCT 1 cut(s) 778
SsiI CCGC 9 cut(s) 58, 62, 605, 607, 1920, 1982, 2123, 2223, 2297
SspMI CTAG 5 cut(s) 621, 1241, 1518, 1527, 2130
SstI GAGCTC 2 cut(s) 712, 1158
StuI AGGCCT 1 cut(s) 778
StyD4I CCNGG 7 cut(s) 31, 1356, 1384, 1719, 2003, 2040, 2067
StyI CCWWGG 1 cut(s) 1735
TaaI ACNGT 6 cut(s) 212, 420, 865, 955, 1143, 1991
TaiI ACGT 3 cut(s) 54, 661, 1201
TatI WGTACW 2 cut(s) 590, 2246
TauI GCSGC 2 cut(s) 607, 2126
TfiI GAWTC 9 cut(s) 125, 374, 1093, 1133, 1532, 1607, 1727, 1757, 2464
TscAI CASTG 5 cut(s) 958, 1306, 1427, 1459, 2460
TseFI GTSAC 3 cut(s) 534, 970, 1267
TseI GCWGC 7 cut(s) 344, 476, 479, 653, 665, 1913, 2126
Tsp45I GTSAC 3 cut(s) 534, 970, 1267
TspDTI ATGAA 9 cut(s) 75, 1085, 1146, 1581, 1707, 1886, 2253, 2280, 2435
TspGWI ACGGA 2 cut(s) 66, 2115
TspRI CASTG 5 cut(s) 958, 1306, 1427, 1459, 2460
Tth111I GACNNNGTC 1 cut(s) 50
VpaK11BI GGWCC 1 cut(s) 1613
VspI ATTAAT 1 cut(s) 1868
XapI RAATTY 3 cut(s) 232, 717, 1744
XceI RCATGY 1 cut(s) 2013
XcmI CCANNNNNNNNNTGG 1 cut(s) 553
XhoI CTCGAG 2 cut(s) 98, 1714
XmiI GTMKAC 2 cut(s) 1409, 2365
XspI CTAG 5 cut(s) 621, 1241, 1518, 1527, 2130
ZraI GACGTC 1 cut(s) 52
ZrmI AGTACT 2 cut(s) 592, 2248
Zsp2I ATGCAT 1 cut(s) 678
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.