Rh5AG059800

Wings apart-like protein regulation of heterochromatin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0009354
Physical Location & Seq
Reverse (-)
5098802 .. 5108395
9594 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG059800.1

Sequence Viewer

Length: 2430 bp
ATGATTGTGAGGACCTACGGCCGGCGCAAGGGAGGAGGACCAGTAGGCCTTCCCAGGACCTATTCCGACTCAACCCTAAACGACGCCGTTCGCGGCGACGACGACGATGACCTCAGCAACGACCCGTTTAGAATCTCTCTCTCCCAAGATACCCCCCAATTCAATTTTAATTTCTCGTCGTCGCAAGACTCGTCGTCGCAGTGGTCGCAGTTCGATTCGGAGCCCTACCCCCCCGAATTGAAAAGGCCCAGTTCGCGAAACGGCGACGTTTTGAGGAGGCCGTCGAAGAAGGCGAAGAAGGCCGCGGCGGCGCTGGCGACGGCGACGTTGATGGAGGCGCAGGAGTTCGGGGAGATGATGGAGCACGTGGACGAGGTCAATTTCGCGCTGGATGGGCTGAGGAATGGTCAGCCGGTTCGGATCAGAAGGGCGAGCTTGTTGTCTTTGTTGGGGATTTGCGGCACGCCTCAGCAGAGACGGCTTCTGAGGACTCAGGGGATGGCAAAAACGATAATTGAGGCTATATTGGGTCTCAGTTTTGATGATGCACCTAGCGATCTGGCTGCTGCAACTATTTTCTATGTTTTGACTTGTGATGGTCAGGATGATCCTCTTTTGGAATCACCAAGCTGTATTAATTTTTTGATTAGGTTTTGCAAACCAATTGTCTCAAACATCACTGAAGATAAAGCACCAAAAATTGGACGAAAGCTTTTAGCATTGCGTTTGAGTGCTGACATCTCGCATTGTGCACCAAAAAGATTAGACTCCAGCTCGGCTGCTATTCTTTCCAAAGTACATGAGATCCTTGTTACTTGCAAGGAAATGAAACCAAGTTCTGCTGATGATGGTGAAATGAGCATGCCGGAGTTAAGCCCCAAATGGATAGCTTTGCTTACTATGGAGAAAGCTTGCTTATCTACTATTTCTCTTGAAGAAACAACTGGTACAGTAAGGAAGGCTGGGGGCAACTTTAAGGAAAAATTACGAGAGCTTGGAGGCCTTGATGCTGTCTTTGAGGTGTCCATGACTTGCCATTCCGAAATGGAGGGTTGGCTAAAAGGTAATTCTCCTTCTACTTGGGAAAAGGAAATCAACATGGTGCGGAGCCTGGTTCTGCTTTTGAAATGCTTGAAGATCATGGAAAATGCCACATTCTTGAGTAAAGAAAATCAGAGCCACTTGCTAGAAATGAAAGGAAAGTTGGTTCCCATGGAAAAGCCCATATCTTTCACAGAACTTGTTATAAGTACCATCAGTATCCTATCAGGGCTTTATCTACATAAAAGTTTATCTGCTGCTTCCAATGATGACATGTCTCATAATCTTTCAAATGGGAATGAATATGCTTCTGAGGAAAGCTCCCACAAATGTCAGAGCAATGGATTGGTGTCCACTTCTCGCTCGGTGTTTTCTGTATCCAATTCAGAAACTACCAGCACGTCTATGACTGGCACCTATTTGTTGAAGACAAGACTTAGTTCTTCCAGAAATGGCTCATCTAGTGGTACAGCAAGCCATTTAAGTGAGGCAACCCGCACATTTAGCTATGGTTCCCGGAAAAATGCTGGTTCCAGTCAGAGATCCTATATTTCTAAAAACTCTATAATTGATCTTTCGGAGGAGAGCCAAGATCCTTTTGCATTTAGCTGTGGTTCCGGTGAGGATGCTGGTCTCAGTCAGAGATCCTATATTTCTGAAGACTCTAAAGTTGACCTTTCACAGGAGAGTCAAGATCCTTTTGCATTTGATGAAGATGATATTGAACCTTCTCAGTGGGACATATTATCTGGCAAGAAAAAGATTTGTCGAACGCGAAAAAATGGAGAAGCTTACAGAGAACTTGATGATGGTTGCCAGTTGCAGCTGATTATGAGCCAAGCAGAATCAAGTTTTGGGGAAGACCATGATATGCCTGAGACATCCTATGCGGGTGCTGTTAGTAAAGAGGGTTCCAGTCTTTTGGCTGACTGCCTTCTTACTGCTGTTAAGGTTCTGATGAACTTGGCAAATGAGAATCCTGTTGGTTGTCAGCAAATTGCTGCAAATGGAGGACTGGAGACCATGTCTTCCTTGATTGCTAGCCACTTTCCTTCATTTTCATCGCCCTTCAGCGAGAGAAATGACAATACTTCAAGCATTGAACTGGATAATCAGAATGGTAGACATCTTACCGATCAAGAGCTAGATTTTCTTGTAGCTATTTTGGGCCTGCTTGTAAACTTGATTGAGAAAGATGGTCAAAACAGATCACGGCTCGCTGCAGTCACTGTTCACTTACCTATTTCTGATGGGTTTGAAGAGGAAAGTCACAAGGATCTAATTCTGCTAATATGCTCTATATTCCTGGCTAACCAAGGTGCAGGCGAGGGATCCGAAGAAGGAAAAACTTTGCCAGTGAGTTCACATTGTCTATGGAAAATTTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WAPL

Protein Analysis

809

Amino Acids

88.29

Weight (kDa)

5.14

Isoelectric Point (pI)

54.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WAPL PF07814 109 - 495 5.8e-47 Wings apart-like protein regulation of heterochromatin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1247
AccB1I GGYRCC 1 cut(s) 1454
AccB7I CCANNNNNTGG 1 cut(s) 701
AccI GTMKAC 1 cut(s) 2164
AccII CGCG 5 cut(s) 93, 256, 305, 386, 1816
AciI CCGC 8 cut(s) 93, 303, 305, 308, 459, 1105, 1537, 1931
AcoI YGGCCR 1 cut(s) 19
AcsI RAATTY 1 cut(s) 2421
AcuI CTGAAG 3 cut(s) 702, 1719, 2095
AcvI CACGTG 1 cut(s) 367
AcyI GRCGYC 1 cut(s) 84
AfaI GTAC 4 cut(s) 798, 949, 1252, 1510
AfiI CCNNNNNNNGG 4 cut(s) 21, 28, 701, 1723
AflIII ACRYGT 1 cut(s) 1314
AhdI GACNNNNNGTC 1 cut(s) 193
AjiI CACGTC 1 cut(s) 1443
AjnI CCWGG 3 cut(s) 53, 1110, 2346
AjuI GAANNNNNNNTTGG 4 cut(s) 1187, 1219, 1878, 1910
AloI GAACNNNNNNTCC 2 cut(s) 1191, 1223
Alw21I GWGCWC 2 cut(s) 366, 754
Alw26I GTCTC 7 cut(s) 469, 536, 673, 1323, 1679, 1913, 2054
Alw44I GTGCAC 1 cut(s) 750
AlwNI CAGNNNCTG 1 cut(s) 2270
AoxI GGCC 7 cut(s) 19, 46, 245, 278, 300, 1000, 2209
ApaLI GTGCAC 1 cut(s) 750
ApeKI GCWGC 7 cut(s) 563, 566, 779, 1298, 1864, 2042, 2261
ApoI RAATTY 1 cut(s) 2421
ArsI GACNNNNNNTTYG 2 cut(s) 651, 683
AseI ATTAAT 1 cut(s) 636
AspLEI GCGC 4 cut(s) 27, 313, 340, 388
AspS9I GGNCC 5 cut(s) 12, 38, 57, 246, 2209
AsuC2I CCSGG 1 cut(s) 1558
AsuHPI GGTGA 3 cut(s) 615, 863, 1673
AsuNHI GCTAGC 1 cut(s) 2081
AvaII GGWCC 3 cut(s) 12, 38, 57
BaeGI GKGCMC 1 cut(s) 754
BamHI GGATCC 1 cut(s) 2372
BanI GGYRCC 1 cut(s) 1454
BanII GRGCYC 1 cut(s) 225
BarI GAAGNNNNNNTAC 2 cut(s) 1057, 1089
BbrPI CACGTG 1 cut(s) 367
BbsI GAAGAC 4 cut(s) 1475, 1707, 1908, 2061
Bbv12I GWGCWC 2 cut(s) 366, 754
BbvCI CCTCAGC 3 cut(s) 113, 398, 468
BbvI GCAGC 7 cut(s) 550, 553, 766, 1285, 1876, 2029, 2248
BceAI ACGGC 7 cut(s) 34, 71, 265, 277, 336, 494, 2270
BcgI CGANNNNNNTGC 2 cut(s) 545, 579
BciT130I CCWGG 3 cut(s) 55, 1112, 2348
BciVI GTATCC 2 cut(s) 1271, 1429
BcnI CCSGG 1 cut(s) 1558
BcoDI GTCTC 7 cut(s) 469, 536, 673, 1323, 1679, 1913, 2054
BfaI CTAG 5 cut(s) 552, 1187, 1503, 2082, 2186
BfmI CTRYAG 1 cut(s) 2262
BfoI RGCGCY 1 cut(s) 314
BfuI GTATCC 2 cut(s) 1271, 1429
BglI GCCNNNNNGGC 1 cut(s) 308
Bme1390I CCNGG 4 cut(s) 55, 1112, 1558, 2348
Bme18I GGWCC 3 cut(s) 12, 38, 57
BmeRI GACNNNNNGTC 1 cut(s) 193
BmgBI CACGTC 1 cut(s) 1443
BmgT120I GGNCC 5 cut(s) 12, 38, 57, 246, 2209
BmiI GGNNCC 9 cut(s) 222, 1109, 1209, 1456, 1555, 1573, 1657, 1954, 2374
BmrFI CCNGG 4 cut(s) 55, 1112, 1558, 2348
BmrI ACTGGG 1 cut(s) 243
BmsI GCATC 3 cut(s) 535, 997, 1657
BmtI GCTAGC 1 cut(s) 2085
BmuI ACTGGG 1 cut(s) 243
BpiI GAAGAC 4 cut(s) 1475, 1707, 1908, 2061
BplI GAGNNNNNCTC 2 cut(s) 1346, 1378
BpmI CTGGAG 2 cut(s) 754, 2078
Bpu10I CCTNAGC 3 cut(s) 113, 398, 468
BpuEI CTTGAG 1 cut(s) 1180
BpuMI CCSGG 1 cut(s) 1558
BsaAI YACGTR 1 cut(s) 367
BsaHI GRCGYC 1 cut(s) 84
BsaI GGTCTC 3 cut(s) 536, 1679, 2054
BsaJI CCNNGG 4 cut(s) 53, 303, 1212, 2356
BsaWI WCCGGW 1 cut(s) 1658
BsaXI ACNNNNNCTCC 4 cut(s) 27, 57, 2051, 2081
Bsc4I CCNNNNNNNGG 4 cut(s) 21, 28, 701, 1723
Bse118I RCCGGY 2 cut(s) 21, 412
Bse3DI GCAATG 2 cut(s) 719, 1387
BseBI CCWGG 3 cut(s) 55, 1112, 2348
BseDI CCNNGG 4 cut(s) 53, 303, 1212, 2356
BseGI GGATG 5 cut(s) 397, 504, 610, 1672, 1922
BseLI CCNNNNNNNGG 4 cut(s) 21, 28, 701, 1723
BseMI GCAATG 2 cut(s) 719, 1387
BseRI GAGGAG 3 cut(s) 48, 289, 1637
BseSI GKGCMC 1 cut(s) 754
BseX3I CGGCCG 1 cut(s) 19
BseXI GCAGC 7 cut(s) 550, 553, 766, 1285, 1876, 2029, 2248
BseYI CCCAGC 1 cut(s) 962
BsgI GTGCAG 1 cut(s) 2382
Bsh1236I CGCG 5 cut(s) 93, 256, 305, 386, 1816
Bsh1285I CGRYCG 1 cut(s) 22
BshFI GGCC 7 cut(s) 21, 48, 247, 280, 302, 1002, 2211
BshNI GGYRCC 1 cut(s) 1454
BsiEI CGRYCG 1 cut(s) 22
BsiHKAI GWGCWC 2 cut(s) 366, 754
BsiSI CCGG 5 cut(s) 22, 413, 866, 1558, 1659
BslFI GGGAC 1 cut(s) 1793
BslI CCNNNNNNNGG 4 cut(s) 21, 28, 701, 1723
BsmAI GTCTC 7 cut(s) 469, 536, 673, 1323, 1679, 1913, 2054
BsmBI CGTCTC 1 cut(s) 469
BsmFI GGGAC 1 cut(s) 1793
BsnI GGCC 7 cut(s) 21, 48, 247, 280, 302, 1002, 2211
Bso31I GGTCTC 3 cut(s) 536, 1679, 2054
Bsp1286I GDGCHC 3 cut(s) 225, 366, 754
Bsp19I CCATGG 1 cut(s) 1212
Bsp68I TCGCGA 1 cut(s) 256
BspACI CCGC 8 cut(s) 93, 303, 305, 308, 459, 1105, 1537, 1931
BspANI GGCC 7 cut(s) 21, 48, 247, 280, 302, 1002, 2211
BspFNI CGCG 5 cut(s) 93, 256, 305, 386, 1816
BspLI GGNNCC 9 cut(s) 222, 1109, 1209, 1456, 1555, 1573, 1657, 1954, 2374
BspMAI CTGCAG 1 cut(s) 2266
BspOI GCTAGC 1 cut(s) 2085
BspT107I GGYRCC 1 cut(s) 1454
BspTNI GGTCTC 3 cut(s) 536, 1679, 2054
BsrDI GCAATG 2 cut(s) 719, 1387
BsrFI RCCGGY 2 cut(s) 21, 412
BssAI RCCGGY 2 cut(s) 21, 412
BssECI CCNNGG 4 cut(s) 53, 303, 1212, 2356
BssNI GRCGYC 1 cut(s) 84
BssT1I CCWWGG 2 cut(s) 1212, 2356
Bst2UI CCWGG 3 cut(s) 55, 1112, 2348
Bst4CI ACNGT 2 cut(s) 952, 2272
Bst6I CTCTTC 1 cut(s) 2295
BstACI GRCGYC 1 cut(s) 84
BstBAI YACGTR 1 cut(s) 367
BstDSI CCRYGG 2 cut(s) 303, 1212
BstENI CCTNNNNNAGG 1 cut(s) 1721
BstF5I GGATG 5 cut(s) 397, 504, 610, 1672, 1922
BstFNI CGCG 5 cut(s) 93, 256, 305, 386, 1816
BstH2I RGCGCY 1 cut(s) 314
BstHHI GCGC 4 cut(s) 27, 313, 340, 388
BstMAI GTCTC 7 cut(s) 469, 536, 673, 1323, 1679, 1913, 2054
BstMCI CGRYCG 1 cut(s) 22
BstMWI GCNNNNNNNGC 8 cut(s) 205, 253, 299, 308, 314, 394, 478, 1545
BstNI CCWGG 3 cut(s) 55, 1112, 2348
BstNSI RCATGY 2 cut(s) 865, 1318
BstSCI CCNGG 4 cut(s) 53, 1110, 1556, 2346
BstSFI CTRYAG 1 cut(s) 2262
BstSLI GKGCMC 1 cut(s) 754
BstUI CGCG 5 cut(s) 93, 256, 305, 386, 1816
BstV1I GCAGC 7 cut(s) 550, 553, 766, 1285, 1876, 2029, 2248
BstV2I GAAGAC 4 cut(s) 1475, 1707, 1908, 2061
BstX2I RGATCY 7 cut(s) 804, 1583, 1633, 1685, 1735, 2317, 2372
BstXI CCANNNNNNTGG 1 cut(s) 1963
BstYI RGATCY 7 cut(s) 804, 1583, 1633, 1685, 1735, 2317, 2372
BstZI CGGCCG 1 cut(s) 19
BsuI GTATCC 2 cut(s) 1271, 1429
BsuRI GGCC 7 cut(s) 21, 48, 247, 280, 302, 1002, 2211
BtgI CCRYGG 2 cut(s) 303, 1212
BtgZI GCGATG 1 cut(s) 2088
BtrI CACGTC 1 cut(s) 1443
BtsCI GGATG 5 cut(s) 397, 504, 610, 1672, 1922
BtsI GCAGTG 1 cut(s) 206
BtsIMutI CAGTG 5 cut(s) 206, 678, 1781, 2268, 2403
BtuMI TCGCGA 1 cut(s) 256
CaiI CAGNNNCTG 1 cut(s) 2270
CfoI GCGC 4 cut(s) 27, 313, 340, 388
Cfr10I RCCGGY 2 cut(s) 21, 412
Cfr13I GGNCC 5 cut(s) 12, 38, 57, 246, 2209
Cfr42I CCGCGG 1 cut(s) 306
CseI GACGC 1 cut(s) 92
Csp6I GTAC 4 cut(s) 797, 948, 1251, 1509
CviAII CATG 9 cut(s) 800, 862, 1027, 1099, 1141, 1213, 1315, 1907, 2065
CviQI GTAC 4 cut(s) 797, 948, 1251, 1509
DriI GACNNNNNGTC 1 cut(s) 193
EaeI YGGCCR 1 cut(s) 19
EagI CGGCCG 1 cut(s) 19
Eam1104I CTCTTC 1 cut(s) 2295
Eam1105I GACNNNNNGTC 1 cut(s) 193
EarI CTCTTC 1 cut(s) 2295
EclXI CGGCCG 1 cut(s) 19
Eco130I CCWWGG 2 cut(s) 1212, 2356
Eco147I AGGCCT 2 cut(s) 48, 1002
Eco24I GRGCYC 1 cut(s) 225
Eco31I GGTCTC 3 cut(s) 536, 1679, 2054
Eco47I GGWCC 3 cut(s) 12, 38, 57
Eco52I CGGCCG 1 cut(s) 19
Eco57I CTGAAG 3 cut(s) 702, 1719, 2095
Eco72I CACGTG 1 cut(s) 367
EcoNI CCTNNNNNAGG 1 cut(s) 1721
EcoO109I RGGNCCY 2 cut(s) 12, 57
EcoRII CCWGG 3 cut(s) 53, 1110, 2346
EcoT14I CCWWGG 2 cut(s) 1212, 2356
EcoT38I GRGCYC 1 cut(s) 225
ErhI CCWWGG 2 cut(s) 1212, 2356
Esp3I CGTCTC 1 cut(s) 469
FaeI CATG 9 cut(s) 803, 865, 1030, 1102, 1144, 1216, 1318, 1910, 2068
FalI AAGNNNNNCTT 2 cut(s) 1701, 1733
FaqI GGGAC 1 cut(s) 1793
FatI CATG 9 cut(s) 799, 861, 1026, 1098, 1140, 1212, 1314, 1906, 2064
FauI CCCGC 2 cut(s) 1544, 1924
FblI GTMKAC 1 cut(s) 2164
FokI GGATG 5 cut(s) 404, 511, 617, 1679, 1909
FriOI GRGCYC 1 cut(s) 225
FspBI CTAG 5 cut(s) 552, 1187, 1503, 2082, 2186
GlaI GCGC 4 cut(s) 26, 312, 339, 387
GsaI CCCAGC 1 cut(s) 966
GsuI CTGGAG 2 cut(s) 754, 2078
HaeII RGCGCY 1 cut(s) 314
HaeIII GGCC 7 cut(s) 21, 48, 247, 280, 302, 1002, 2211
HapII CCGG 5 cut(s) 22, 413, 866, 1558, 1659
HgaI GACGC 1 cut(s) 92
HhaI GCGC 4 cut(s) 27, 313, 340, 388
Hin1I GRCGYC 1 cut(s) 84
Hin1II CATG 9 cut(s) 803, 865, 1030, 1102, 1144, 1216, 1318, 1910, 2068
Hin6I GCGC 4 cut(s) 25, 311, 338, 386
HinP1I GCGC 4 cut(s) 25, 311, 338, 386
HincII GTYRAC 1 cut(s) 1714
HindII GTYRAC 1 cut(s) 1714
HindIII AAGCTT 3 cut(s) 710, 909, 1830
HpaII CCGG 5 cut(s) 22, 413, 866, 1558, 1659
HphI GGTGA 3 cut(s) 615, 863, 1673
Hpy166II GTNNAC 8 cut(s) 370, 752, 1395, 1714, 2165, 2221, 2275, 2405
Hpy188III TCNNGA 7 cut(s) 255, 602, 932, 1159, 1488, 1733, 2180
Hpy8I GTNNAC 8 cut(s) 370, 752, 1395, 1714, 2165, 2221, 2275, 2405
HpyCH4III ACNGT 2 cut(s) 952, 2272
HpyCH4IV ACGT 4 cut(s) 267, 326, 366, 1442
HpyF10VI GCNNNNNNNGC 8 cut(s) 205, 253, 299, 308, 314, 394, 478, 1545
HpySE526I ACGT 4 cut(s) 267, 326, 366, 1442
Hsp92I GRCGYC 1 cut(s) 84
Hsp92II CATG 9 cut(s) 803, 865, 1030, 1102, 1144, 1216, 1318, 1910, 2068
HspAI GCGC 4 cut(s) 25, 311, 338, 386
KroI GCCGGC 1 cut(s) 21
KroNI GCCGGC 1 cut(s) 23
KspI CCGCGG 1 cut(s) 306
LmnI GCTCC 4 cut(s) 220, 361, 1107, 1367
Lsp1109I GCAGC 7 cut(s) 550, 553, 766, 1285, 1876, 2029, 2248
LweI GCATC 3 cut(s) 535, 997, 1657
MaeI CTAG 5 cut(s) 552, 1187, 1503, 2082, 2186
MaeII ACGT 4 cut(s) 267, 326, 366, 1442
MaeIII GTNAC 3 cut(s) 811, 2266, 2309
MfeI CAATTG 1 cut(s) 663
MflI RGATCY 7 cut(s) 804, 1583, 1633, 1685, 1735, 2317, 2372
MhlI GDGCHC 3 cut(s) 225, 366, 754
MlyI GAGTC 6 cut(s) 62, 182, 484, 761, 1697, 1738
MmeI TCCRAC 1 cut(s) 90
MroNI GCCGGC 1 cut(s) 21
MseI TTAA 6 cut(s) 168, 636, 872, 975, 1523, 1989
MslI CAYNNNNRTG 2 cut(s) 1445, 1524
MspA1I CMGCKG 2 cut(s) 305, 1867
MspI CCGG 5 cut(s) 22, 413, 866, 1558, 1659
MspR9I CCNGG 4 cut(s) 55, 1112, 1558, 2348
MunI CAATTG 1 cut(s) 663
MvaI CCWGG 3 cut(s) 55, 1112, 2348
MvnI CGCG 5 cut(s) 93, 256, 305, 386, 1816
MwoI GCNNNNNNNGC 8 cut(s) 205, 253, 299, 308, 314, 394, 478, 1545
NaeI GCCGGC 1 cut(s) 23
NciI CCSGG 1 cut(s) 1558
NcoI CCATGG 1 cut(s) 1212
NgoMIV GCCGGC 1 cut(s) 21
NheI GCTAGC 1 cut(s) 2081
NlaIII CATG 9 cut(s) 803, 865, 1030, 1102, 1144, 1216, 1318, 1910, 2068
NlaIV GGNNCC 9 cut(s) 222, 1109, 1209, 1456, 1555, 1573, 1657, 1954, 2374
NmeAIII GCCGAG 1 cut(s) 755
NmuCI GTSAC 2 cut(s) 2266, 2309
NruI TCGCGA 1 cut(s) 256
NspI RCATGY 2 cut(s) 865, 1318
PaeI GCATGC 1 cut(s) 865
PceI AGGCCT 2 cut(s) 48, 1002
PciI ACATGT 1 cut(s) 1314
PcsI WCGNNNNNNNCGW 2 cut(s) 188, 290
PdiI GCCGGC 1 cut(s) 23
PfeI GAWTC 5 cut(s) 132, 215, 620, 1886, 2017
PflFI GACNNNGTC 2 cut(s) 374, 2065
PflMI CCANNNNNTGG 1 cut(s) 701
PfoI TCCNGGA 1 cut(s) 1556
PleI GAGTC 6 cut(s) 62, 182, 484, 761, 1697, 1737
PmaCI CACGTG 1 cut(s) 367
PmlI CACGTG 1 cut(s) 367
PpsI GAGTC 6 cut(s) 62, 182, 484, 761, 1697, 1737
Ppu21I YACGTR 1 cut(s) 367
PpuMI RGGWCCY 2 cut(s) 12, 57
PscI ACATGT 1 cut(s) 1314
PshBI ATTAAT 1 cut(s) 636
PsiI TTATAA 1 cut(s) 1247
Psp5II RGGWCCY 2 cut(s) 12, 57
Psp6I CCWGG 3 cut(s) 53, 1110, 2346
PspCI CACGTG 1 cut(s) 367
PspFI CCCAGC 1 cut(s) 962
PspGI CCWGG 3 cut(s) 53, 1110, 2346
PspN4I GGNNCC 9 cut(s) 222, 1109, 1209, 1456, 1555, 1573, 1657, 1954, 2374
PspPI GGNCC 5 cut(s) 12, 38, 57, 246, 2209
PspPPI RGGWCCY 2 cut(s) 12, 57
PsrI GAACNNNNNNTAC 2 cut(s) 1936, 1968
PstI CTGCAG 1 cut(s) 2266
PstNI CAGNNNCTG 1 cut(s) 2270
PsuI RGATCY 7 cut(s) 804, 1583, 1633, 1685, 1735, 2317, 2372
PsyI GACNNNGTC 2 cut(s) 374, 2065
PvuII CAGCTG 1 cut(s) 1867
RruI TCGCGA 1 cut(s) 256
RsaI GTAC 4 cut(s) 798, 949, 1252, 1510
RsaNI GTAC 4 cut(s) 797, 948, 1251, 1509
RseI CAYNNNNRTG 2 cut(s) 1445, 1524
SacII CCGCGG 1 cut(s) 306
SaqAI TTAA 6 cut(s) 168, 636, 872, 975, 1523, 1989
Sau96I GGNCC 5 cut(s) 12, 38, 57, 246, 2209
SchI GAGTC 6 cut(s) 62, 182, 484, 761, 1697, 1738
ScrFI CCNGG 4 cut(s) 55, 1112, 1558, 2348
SduI GDGCHC 3 cut(s) 225, 366, 754
SfaNI GCATC 3 cut(s) 535, 997, 1657
SfcI CTRYAG 1 cut(s) 2262
Sfr303I CCGCGG 1 cut(s) 306
SgrBI CCGCGG 1 cut(s) 306
SinI GGWCC 3 cut(s) 12, 38, 57
SmiMI CAYNNNNRTG 2 cut(s) 1445, 1524
SmlI CTYRAG 1 cut(s) 1159
SmoI CTYRAG 1 cut(s) 1159
SphI GCATGC 1 cut(s) 865
SseBI AGGCCT 2 cut(s) 48, 1002
SsiI CCGC 8 cut(s) 93, 303, 305, 308, 459, 1105, 1537, 1931
SspMI CTAG 5 cut(s) 552, 1187, 1503, 2082, 2186
StuI AGGCCT 2 cut(s) 48, 1002
StyD4I CCNGG 4 cut(s) 53, 1110, 1556, 2346
StyI CCWWGG 2 cut(s) 1212, 2356
TaaI ACNGT 2 cut(s) 952, 2272
TaiI ACGT 4 cut(s) 270, 329, 369, 1445
TaqI TCGA 3 cut(s) 213, 284, 1810
TatI WGTACW 1 cut(s) 796
TauI GCSGC 5 cut(s) 96, 305, 308, 311, 462
TfiI GAWTC 5 cut(s) 132, 215, 620, 1886, 2017
Tru1I TTAA 6 cut(s) 168, 636, 872, 975, 1523, 1989
Tru9I TTAA 6 cut(s) 168, 636, 872, 975, 1523, 1989
TscAI CASTG 5 cut(s) 206, 685, 1781, 2275, 2403
TseFI GTSAC 2 cut(s) 2266, 2309
TseI GCWGC 7 cut(s) 563, 566, 779, 1298, 1864, 2042, 2261
Tsp45I GTSAC 2 cut(s) 2266, 2309
TspDTI ATGAA 7 cut(s) 842, 1208, 1356, 1767, 2015, 2085, 2091
TspRI CASTG 5 cut(s) 206, 685, 1781, 2275, 2403
Tth111I GACNNNGTC 2 cut(s) 374, 2065
Van91I CCANNNNNTGG 1 cut(s) 701
VneI GTGCAC 1 cut(s) 750
VpaK11BI GGWCC 3 cut(s) 12, 38, 57
VspI ATTAAT 1 cut(s) 636
XagI CCTNNNNNAGG 1 cut(s) 1721
XapI RAATTY 1 cut(s) 2421
XceI RCATGY 2 cut(s) 865, 1318
XmiI GTMKAC 1 cut(s) 2164
XspI CTAG 5 cut(s) 552, 1187, 1503, 2082, 2186
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.