RLG00000031502

Wings apart-like protein regulation of heterochromatin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0009354
Physical Location & Seq
Reverse (-)
5656193 .. 5662544
6352 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031502

Sequence Viewer

Length: 2661 bp
ATGATTGTGAGGACCTACGGCCGGCGCAAGGGAGGAGGACCAGTAGGGCTTCCCAGGACCTATTCCGACTCAACCCTAAACGACGCCGTTCGCGGTGACGACGACGACGACCTCAGCACCGACCCGTTTAGAATCTCTCTCTCCCAAGATACCCCCCAATTCAATTTTAATTTCTCGTCGTCGCAAGACTCGTCGTCGCAGTGGTCGCAGTTCGATTCGGAGCCCTATCCCCCCGAATTGAAAAGGCCCAGTTCGCGAAACGGCGACGTTTTGAGGAGGCCGTCGAAGAAGGCGAAGAAGGCCGCGGTGGCGCTGGCGACGGCGACGTTGATGGAGGCGCAGGAGTTCGGGGAGATGATGGAGCACGTGGACGAGGTCAATTTCGCGCTGGATGGGCTGAGGAATGGTCAGCCGGTTCGGATCAGAAGGGCGAGCTTGTTGTCTTTGTTGGGGATTTGTGGCACGCCGCAACAGAGACGGCTTCTGAGGACTCAGGGGATGGCAAAAACGATAATTGAGGCTATATTGGGTCTCAGTTTTGATGATACACCTTGCGATCTGGCTGCTGCGACTATTTTCTATGTTTTGACTTGTGATGGTCAGGATGATCCTCTTTTGGAATCACCAAGCTGTATTAATTTTCTGATTAGGTTTTGCAAACCAGTTGTCTCAAACATCACTGAAGATAAAGCACCAAAACTTGGACGAAAGCTTTTAGCATTGCGTTTGAGTGCTGACATCTCGCATTGTGTACCAAAAAGATTAGACTCCAGCTCGGCTGCTATTCTTTCCAAAGTACATGAGATTCTTGTTACTTGCAAGGAAATGAAACCAAGTTCTGCTGATGATGGTGAAATGAGCATGCCGGAGTTAAGCGCCAAATGGATAGCTTTGCTTACTATGGAGAAAGCTTGCTTATCTACTATTTCTCTTGAAGAAACAACTGGTACAGTAAGGAAGGCTGGGGGCAACTTTAAGGAAAAATTACGAGAGCTTGGAGGCCTTGATGCTGTCTTTGAGGTGTCCATGACTTGCCATTCCGAAATGGAGGGTTGGCTAAAAGGTAATTCTCCTTCTACTTGGGAAAAGGAAATCAACATGGTGCGGAGCCTGGTTCTGCTTTTGAAATGCTTGAAGATCATGGAAAATGCCACATTCTTGAGTAAAGAAAATCAGAGCCACTTGCTAGAAATGAAAGGAAAGTTGAATCCCGTGGAAAAGCCCATATCTTTCACAGAACTTGTTATAAGTACCATCAGTATCCTATCAGGGCTTTATCTACATAAAAGTTTATCTGCTGCCTCCAATGATGCCATGTCTCATAATCTTTCAAATGGGAATGAATATGCTTCTCAGAAAAGCTCCCACAAATGTCAGAGCAATGGATTGGTGTCCACTTCTCGCTCGGTGTATTCTGTATCCAGTTCAGAAACTACCAGCACGTCTATGACTGACACCTATTCGTTGAAGACAAGACTTAGTTCTTCCAGAAATGGCTCATCTAGTGGTACAGCAAGCCATTTAAGTGAGGCAACCCGCACATTTATCTATGGTTCCCGGAAAGATGCTGGTTCCAGTCAGAGATCCTATATTTCTAAAAACTCTATAATTGATCTTTCGGAGGAGAGCCAAGATCCTTTTGCATTTATCTGTGGTTCCGGTGAGGATGCTGGTCTCAGTCAGAGATCCTATATTTCTGAAGACTCTAAAGTTGACCTTTCACAGGAGAGTCAAGATCCTTTTGCATTTGATGAAGATGATATTGAACCTTCTCAGTGGGACATATTATCTGGCAAGAAAAAGATTTGTCGAACGCGAAAACATGGAGAAGCTTACAGAGAACTTGATGATGGTTGCCAGTTGCAGCTGATTATGAGCCAAACAGAATCAAGTATTGGGGAAGACCATGATCTGCCTGAGACATCCTATGCGGGTGCTGTTAGTAAAGAGGGTTCCAGTCTTTTGGCTGACTGCCTTCTTACTGCTGTTAAGGTTCTGATGAACTTGGCAAATGAGAATCCTGTTGGTTGTCAGCAAATTGCTGCGAATGGAGGACTGGAGACCATGTCTTCCTTGATTGCTAGCCACTTTCCTTCATTTTCATCTCCCTTCAGCGAGAGAAATGACAATACTTCAAGCATTGAACTGGATAATCAGAATGGTAGACATCTTACTGATCAAGAGCTAGATTTTCTTGTAGCTATTCTGGGCCTGCTTGTAAACTTGGTTGAGAAAGATGGTCAAAACAGATCACGGCTCGCTGCAGTCACTGTTCACTTACCTATTTCTGATGGGTTTGAAGAGGAAAGTCACAAGGATCTAATTCTGCTAATATGCTCTATATTCCTGGCTAATCAAGGTGCAGGCGAGGGATCCGAAGAAGGAAAAACTTTGCCAAATGATGAAGCAGCTGTGTTACAAGGAGAACAAGAAGCTGAGAAAATGATAGTGGAAGCTTATGCTGCCCTGCTTTTAGCATTCCTTTCAACTGAAAGTAAGGGTGTACGCGATGCCATTGCTGACTGTCTCCCAGATCGCAACCTGGCTATCCTAGTACCTGTGTTGGACAGATTTGTGGCATTTCACTTGACGTTAAACATGATCTCACCGGAGACTCATAAAGTCGTAAGTGAAGTAATTGAATCATGTAGGATACGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WAPL

Protein Analysis

887

Amino Acids

96.79

Weight (kDa)

5.09

Isoelectric Point (pI)

53.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WAPL PF07814 109 - 747 5.8e-50 Wings apart-like protein regulation of heterochromatin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1247
AccB7I CCANNNNNTGG 1 cut(s) 701
AccI GTMKAC 1 cut(s) 2164
AccII CGCG 6 cut(s) 93, 256, 305, 386, 1816, 2508
AciI CCGC 7 cut(s) 93, 303, 305, 467, 1105, 1537, 1931
AclWI GGATC 9 cut(s) 428, 602, 1578, 1628, 1680, 1730, 2325, 2367, 2380
AcoI YGGCCR 1 cut(s) 19
AcuI CTGAAG 3 cut(s) 702, 1719, 2095
AcvI CACGTG 1 cut(s) 367
AcyI GRCGYC 1 cut(s) 84
AfaI GTAC 7 cut(s) 753, 798, 949, 1252, 1510, 2505, 2556
AfiI CCNNNNNNNGG 4 cut(s) 21, 28, 701, 1723
AhdI GACNNNNNGTC 1 cut(s) 193
AjiI CACGTC 1 cut(s) 1443
AjnI CCWGG 4 cut(s) 53, 1110, 2346, 2541
AjuI GAANNNNNNNTTGG 2 cut(s) 1878, 1910
Alw21I GWGCWC 1 cut(s) 366
Alw26I GTCTC 9 cut(s) 469, 536, 673, 1323, 1679, 1913, 2054, 2531, 2606
AlwI GGATC 9 cut(s) 428, 602, 1578, 1628, 1680, 1730, 2325, 2367, 2380
AlwNI CAGNNNCTG 1 cut(s) 2270
AoxI GGCC 6 cut(s) 19, 245, 278, 300, 1000, 2209
ApeKI GCWGC 9 cut(s) 563, 566, 779, 1298, 1864, 2042, 2261, 2408, 2462
ArsI GACNNNNNNTTYG 2 cut(s) 651, 683
AseI ATTAAT 1 cut(s) 636
AspLEI GCGC 5 cut(s) 27, 313, 340, 388, 878
AspS9I GGNCC 5 cut(s) 12, 38, 57, 246, 2209
AsuC2I CCSGG 1 cut(s) 1558
AsuHPI GGTGA 5 cut(s) 107, 615, 863, 1673, 2598
AsuNHI GCTAGC 1 cut(s) 2081
AvaII GGWCC 3 cut(s) 12, 38, 57
BamHI GGATCC 1 cut(s) 2372
BanII GRGCYC 1 cut(s) 225
BarI GAAGNNNNNNTAC 2 cut(s) 1057, 1089
BbrPI CACGTG 1 cut(s) 367
BbsI GAAGAC 4 cut(s) 1475, 1707, 1908, 2061
Bbv12I GWGCWC 1 cut(s) 366
BbvCI CCTCAGC 2 cut(s) 113, 398
BbvI GCAGC 9 cut(s) 550, 553, 766, 1285, 1876, 2029, 2248, 2420, 2449
BceAI ACGGC 7 cut(s) 34, 71, 265, 277, 336, 494, 2270
BcgI CGANNNNNNTGC 4 cut(s) 545, 579, 2498, 2532
BciT130I CCWGG 4 cut(s) 55, 1112, 2348, 2543
BciVI GTATCC 3 cut(s) 1271, 1429, 2646
BclI TGATCA 1 cut(s) 2176
BcnI CCSGG 1 cut(s) 1558
BcoDI GTCTC 9 cut(s) 469, 536, 673, 1323, 1679, 1913, 2054, 2531, 2606
BfaI CTAG 5 cut(s) 1187, 1503, 2082, 2186, 2552
BfmI CTRYAG 1 cut(s) 2262
BfoI RGCGCY 2 cut(s) 314, 879
BfuI GTATCC 3 cut(s) 1271, 1429, 2646
BglI GCCNNNNNGGC 1 cut(s) 308
Bme1390I CCNGG 5 cut(s) 55, 1112, 1558, 2348, 2543
Bme18I GGWCC 3 cut(s) 12, 38, 57
BmeRI GACNNNNNGTC 1 cut(s) 193
BmgBI CACGTC 1 cut(s) 1443
BmgT120I GGNCC 5 cut(s) 12, 38, 57, 246, 2209
BmiI GGNNCC 7 cut(s) 222, 1109, 1555, 1573, 1657, 1954, 2374
BmrFI CCNGG 5 cut(s) 55, 1112, 1558, 2348, 2543
BmrI ACTGGG 1 cut(s) 243
BmsI GCATC 5 cut(s) 997, 1300, 1555, 1657, 2500
BmtI GCTAGC 1 cut(s) 2085
BmuI ACTGGG 1 cut(s) 243
BpiI GAAGAC 4 cut(s) 1475, 1707, 1908, 2061
BpmI CTGGAG 2 cut(s) 754, 2078
Bpu10I CCTNAGC 2 cut(s) 113, 398
BpuEI CTTGAG 1 cut(s) 1180
BpuMI CCSGG 1 cut(s) 1558
BsaAI YACGTR 1 cut(s) 367
BsaHI GRCGYC 1 cut(s) 84
BsaI GGTCTC 3 cut(s) 536, 1679, 2054
BsaJI CCNNGG 3 cut(s) 53, 303, 1212
BsaWI WCCGGW 2 cut(s) 1658, 2608
BsaXI ACNNNNNCTCC 4 cut(s) 27, 57, 2051, 2081
Bsc4I CCNNNNNNNGG 4 cut(s) 21, 28, 701, 1723
Bse118I RCCGGY 2 cut(s) 21, 412
Bse3DI GCAATG 3 cut(s) 719, 1387, 2514
BseBI CCWGG 4 cut(s) 55, 1112, 2348, 2543
BseDI CCNNGG 3 cut(s) 53, 303, 1212
BseGI GGATG 5 cut(s) 397, 504, 610, 1672, 1922
BseLI CCNNNNNNNGG 4 cut(s) 21, 28, 701, 1723
BseMI GCAATG 3 cut(s) 719, 1387, 2514
BseRI GAGGAG 3 cut(s) 48, 289, 1637
BseX3I CGGCCG 1 cut(s) 19
BseXI GCAGC 9 cut(s) 550, 553, 766, 1285, 1876, 2029, 2248, 2420, 2449
BseYI CCCAGC 1 cut(s) 962
BsgI GTGCAG 1 cut(s) 2382
Bsh1236I CGCG 6 cut(s) 93, 256, 305, 386, 1816, 2508
Bsh1285I CGRYCG 1 cut(s) 22
BshFI GGCC 6 cut(s) 21, 247, 280, 302, 1002, 2211
BsiEI CGRYCG 1 cut(s) 22
BsiHKAI GWGCWC 1 cut(s) 366
BsiSI CCGG 6 cut(s) 22, 413, 866, 1558, 1659, 2609
BslFI GGGAC 1 cut(s) 1793
BslI CCNNNNNNNGG 4 cut(s) 21, 28, 701, 1723
BsmAI GTCTC 9 cut(s) 469, 536, 673, 1323, 1679, 1913, 2054, 2531, 2606
BsmBI CGTCTC 1 cut(s) 469
BsmFI GGGAC 1 cut(s) 1793
BsmI GAATGC 1 cut(s) 2477
BsnI GGCC 6 cut(s) 21, 247, 280, 302, 1002, 2211
Bso31I GGTCTC 3 cut(s) 536, 1679, 2054
Bsp1286I GDGCHC 2 cut(s) 225, 366
Bsp68I TCGCGA 1 cut(s) 256
BspACI CCGC 7 cut(s) 93, 303, 305, 467, 1105, 1537, 1931
BspANI GGCC 6 cut(s) 21, 247, 280, 302, 1002, 2211
BspFNI CGCG 6 cut(s) 93, 256, 305, 386, 1816, 2508
BspLI GGNNCC 7 cut(s) 222, 1109, 1555, 1573, 1657, 1954, 2374
BspMAI CTGCAG 1 cut(s) 2266
BspOI GCTAGC 1 cut(s) 2085
BspPI GGATC 9 cut(s) 428, 602, 1578, 1628, 1680, 1730, 2325, 2367, 2380
BspTNI GGTCTC 3 cut(s) 536, 1679, 2054
BsrDI GCAATG 3 cut(s) 719, 1387, 2514
BsrFI RCCGGY 2 cut(s) 21, 412
BssAI RCCGGY 2 cut(s) 21, 412
BssECI CCNNGG 3 cut(s) 53, 303, 1212
BssNI GRCGYC 1 cut(s) 84
Bst2UI CCWGG 4 cut(s) 55, 1112, 2348, 2543
Bst4CI ACNGT 3 cut(s) 952, 2272, 2525
Bst6I CTCTTC 1 cut(s) 2295
BstACI GRCGYC 1 cut(s) 84
BstBAI YACGTR 1 cut(s) 367
BstDSI CCRYGG 2 cut(s) 303, 1212
BstENI CCTNNNNNAGG 1 cut(s) 1721
BstF5I GGATG 5 cut(s) 397, 504, 610, 1672, 1922
BstFNI CGCG 6 cut(s) 93, 256, 305, 386, 1816, 2508
BstH2I RGCGCY 2 cut(s) 314, 879
BstHHI GCGC 5 cut(s) 27, 313, 340, 388, 878
BstMAI GTCTC 9 cut(s) 469, 536, 673, 1323, 1679, 1913, 2054, 2531, 2606
BstMCI CGRYCG 1 cut(s) 22
BstMWI GCNNNNNNNGC 6 cut(s) 205, 253, 299, 308, 394, 2462
BstNI CCWGG 4 cut(s) 55, 1112, 2348, 2543
BstNSI RCATGY 1 cut(s) 865
BstSCI CCNGG 5 cut(s) 53, 1110, 1556, 2346, 2541
BstSFI CTRYAG 1 cut(s) 2262
BstUI CGCG 6 cut(s) 93, 256, 305, 386, 1816, 2508
BstV1I GCAGC 9 cut(s) 550, 553, 766, 1285, 1876, 2029, 2248, 2420, 2449
BstV2I GAAGAC 4 cut(s) 1475, 1707, 1908, 2061
BstX2I RGATCY 6 cut(s) 1583, 1633, 1685, 1735, 2317, 2372
BstXI CCANNNNNNTGG 1 cut(s) 1963
BstYI RGATCY 6 cut(s) 1583, 1633, 1685, 1735, 2317, 2372
BstZI CGGCCG 1 cut(s) 19
BsuI GTATCC 3 cut(s) 1271, 1429, 2646
BsuRI GGCC 6 cut(s) 21, 247, 280, 302, 1002, 2211
BtgI CCRYGG 2 cut(s) 303, 1212
BtgZI GCGATG 1 cut(s) 2523
BtrI CACGTC 1 cut(s) 1443
BtsCI GGATG 5 cut(s) 397, 504, 610, 1672, 1922
BtsI GCAGTG 1 cut(s) 206
BtsIMutI CAGTG 4 cut(s) 206, 678, 1781, 2268
BtuMI TCGCGA 1 cut(s) 256
CaiI CAGNNNCTG 1 cut(s) 2270
CfoI GCGC 5 cut(s) 27, 313, 340, 388, 878
Cfr10I RCCGGY 2 cut(s) 21, 412
Cfr13I GGNCC 5 cut(s) 12, 38, 57, 246, 2209
Cfr42I CCGCGG 1 cut(s) 306
CseI GACGC 1 cut(s) 92
Csp6I GTAC 7 cut(s) 752, 797, 948, 1251, 1509, 2504, 2555
CviQI GTAC 7 cut(s) 752, 797, 948, 1251, 1509, 2504, 2555
DriI GACNNNNNGTC 1 cut(s) 193
EaeI YGGCCR 1 cut(s) 19
EagI CGGCCG 1 cut(s) 19
Eam1104I CTCTTC 1 cut(s) 2295
Eam1105I GACNNNNNGTC 1 cut(s) 193
EarI CTCTTC 1 cut(s) 2295
EclXI CGGCCG 1 cut(s) 19
Eco147I AGGCCT 1 cut(s) 1002
Eco24I GRGCYC 1 cut(s) 225
Eco31I GGTCTC 3 cut(s) 536, 1679, 2054
Eco47I GGWCC 3 cut(s) 12, 38, 57
Eco52I CGGCCG 1 cut(s) 19
Eco57I CTGAAG 3 cut(s) 702, 1719, 2095
Eco72I CACGTG 1 cut(s) 367
EcoNI CCTNNNNNAGG 1 cut(s) 1721
EcoO109I RGGNCCY 2 cut(s) 12, 57
EcoRII CCWGG 4 cut(s) 53, 1110, 2346, 2541
EcoT38I GRGCYC 1 cut(s) 225
Esp3I CGTCTC 1 cut(s) 469
FalI AAGNNNNNCTT 2 cut(s) 1701, 1733
FaqI GGGAC 1 cut(s) 1793
FauI CCCGC 2 cut(s) 1544, 1924
FbaI TGATCA 1 cut(s) 2176
FblI GTMKAC 1 cut(s) 2164
FokI GGATG 5 cut(s) 404, 511, 617, 1679, 1909
FriOI GRGCYC 1 cut(s) 225
FspBI CTAG 5 cut(s) 1187, 1503, 2082, 2186, 2552
GlaI GCGC 5 cut(s) 26, 312, 339, 387, 877
GsaI CCCAGC 1 cut(s) 966
GsuI CTGGAG 2 cut(s) 754, 2078
HaeII RGCGCY 2 cut(s) 314, 879
HaeIII GGCC 6 cut(s) 21, 247, 280, 302, 1002, 2211
HapII CCGG 6 cut(s) 22, 413, 866, 1558, 1659, 2609
HgaI GACGC 1 cut(s) 92
HhaI GCGC 5 cut(s) 27, 313, 340, 388, 878
Hin1I GRCGYC 1 cut(s) 84
Hin6I GCGC 5 cut(s) 25, 311, 338, 386, 876
HinP1I GCGC 5 cut(s) 25, 311, 338, 386, 876
HincII GTYRAC 1 cut(s) 1714
HindII GTYRAC 1 cut(s) 1714
HindIII AAGCTT 4 cut(s) 710, 909, 1830, 2454
HpaII CCGG 6 cut(s) 22, 413, 866, 1558, 1659, 2609
HphI GGTGA 5 cut(s) 107, 615, 863, 1673, 2598
Hpy166II GTNNAC 8 cut(s) 370, 752, 1395, 1714, 2165, 2221, 2275, 2504
Hpy188III TCNNGA 7 cut(s) 255, 602, 932, 1159, 1488, 1733, 2180
Hpy8I GTNNAC 8 cut(s) 370, 752, 1395, 1714, 2165, 2221, 2275, 2504
HpyCH4III ACNGT 3 cut(s) 952, 2272, 2525
HpyCH4IV ACGT 5 cut(s) 267, 326, 366, 1442, 2591
HpyCH4V TGCA 7 cut(s) 657, 819, 1643, 1745, 1864, 2264, 2363
HpyF10VI GCNNNNNNNGC 6 cut(s) 205, 253, 299, 308, 394, 2462
HpySE526I ACGT 5 cut(s) 267, 326, 366, 1442, 2591
Hsp92I GRCGYC 1 cut(s) 84
HspAI GCGC 5 cut(s) 25, 311, 338, 386, 876
KroI GCCGGC 1 cut(s) 21
KroNI GCCGGC 1 cut(s) 23
Ksp22I TGATCA 1 cut(s) 2176
KspI CCGCGG 1 cut(s) 306
LmnI GCTCC 4 cut(s) 220, 361, 1107, 1367
Lsp1109I GCAGC 9 cut(s) 550, 553, 766, 1285, 1876, 2029, 2248, 2420, 2449
LweI GCATC 5 cut(s) 997, 1300, 1555, 1657, 2500
MaeI CTAG 5 cut(s) 1187, 1503, 2082, 2186, 2552
MaeII ACGT 5 cut(s) 267, 326, 366, 1442, 2591
MaeIII GTNAC 5 cut(s) 95, 811, 2266, 2309, 2415
MflI RGATCY 6 cut(s) 1583, 1633, 1685, 1735, 2317, 2372
MhlI GDGCHC 2 cut(s) 225, 366
MlyI GAGTC 7 cut(s) 62, 182, 484, 761, 1697, 1738, 2608
MmeI TCCRAC 2 cut(s) 90, 2544
MroNI GCCGGC 1 cut(s) 21
MseI TTAA 7 cut(s) 168, 636, 872, 975, 1523, 1989, 2594
MslI CAYNNNNRTG 2 cut(s) 1445, 1524
MspA1I CMGCKG 3 cut(s) 305, 1867, 2411
MspI CCGG 6 cut(s) 22, 413, 866, 1558, 1659, 2609
MspR9I CCNGG 5 cut(s) 55, 1112, 1558, 2348, 2543
Mva1269I GAATGC 1 cut(s) 2477
MvaI CCWGG 4 cut(s) 55, 1112, 2348, 2543
MvnI CGCG 6 cut(s) 93, 256, 305, 386, 1816, 2508
MwoI GCNNNNNNNGC 6 cut(s) 205, 253, 299, 308, 394, 2462
NaeI GCCGGC 1 cut(s) 23
NciI CCSGG 1 cut(s) 1558
NgoMIV GCCGGC 1 cut(s) 21
NheI GCTAGC 1 cut(s) 2081
NlaIV GGNNCC 7 cut(s) 222, 1109, 1555, 1573, 1657, 1954, 2374
NmeAIII GCCGAG 1 cut(s) 755
NmuCI GTSAC 3 cut(s) 95, 2266, 2309
NruI TCGCGA 1 cut(s) 256
NspI RCATGY 1 cut(s) 865
PaeI GCATGC 1 cut(s) 865
PceI AGGCCT 1 cut(s) 1002
PcsI WCGNNNNNNNCGW 3 cut(s) 105, 188, 290
PctI GAATGC 1 cut(s) 2477
PdiI GCCGGC 1 cut(s) 23
PfeI GAWTC 8 cut(s) 132, 215, 620, 805, 1207, 1886, 2017, 2642
PflFI GACNNNGTC 2 cut(s) 374, 2065
PflMI CCANNNNNTGG 1 cut(s) 701
PfoI TCCNGGA 1 cut(s) 1556
PleI GAGTC 7 cut(s) 62, 182, 484, 761, 1697, 1737, 2608
PmaCI CACGTG 1 cut(s) 367
PmlI CACGTG 1 cut(s) 367
PpsI GAGTC 7 cut(s) 62, 182, 484, 761, 1697, 1737, 2608
Ppu21I YACGTR 1 cut(s) 367
PpuMI RGGWCCY 2 cut(s) 12, 57
PshBI ATTAAT 1 cut(s) 636
PsiI TTATAA 1 cut(s) 1247
Psp5II RGGWCCY 2 cut(s) 12, 57
Psp6I CCWGG 4 cut(s) 53, 1110, 2346, 2541
PspCI CACGTG 1 cut(s) 367
PspFI CCCAGC 1 cut(s) 962
PspGI CCWGG 4 cut(s) 53, 1110, 2346, 2541
PspN4I GGNNCC 7 cut(s) 222, 1109, 1555, 1573, 1657, 1954, 2374
PspPI GGNCC 5 cut(s) 12, 38, 57, 246, 2209
PspPPI RGGWCCY 2 cut(s) 12, 57
PsrI GAACNNNNNNTAC 2 cut(s) 1936, 1968
PstI CTGCAG 1 cut(s) 2266
PstNI CAGNNNCTG 1 cut(s) 2270
PsuI RGATCY 6 cut(s) 1583, 1633, 1685, 1735, 2317, 2372
PsyI GACNNNGTC 2 cut(s) 374, 2065
PvuII CAGCTG 2 cut(s) 1867, 2411
RruI TCGCGA 1 cut(s) 256
RsaI GTAC 7 cut(s) 753, 798, 949, 1252, 1510, 2505, 2556
RsaNI GTAC 7 cut(s) 752, 797, 948, 1251, 1509, 2504, 2555
RseI CAYNNNNRTG 2 cut(s) 1445, 1524
SacII CCGCGG 1 cut(s) 306
SaqAI TTAA 7 cut(s) 168, 636, 872, 975, 1523, 1989, 2594
Sau96I GGNCC 5 cut(s) 12, 38, 57, 246, 2209
SchI GAGTC 7 cut(s) 62, 182, 484, 761, 1697, 1738, 2608
ScrFI CCNGG 5 cut(s) 55, 1112, 1558, 2348, 2543
SduI GDGCHC 2 cut(s) 225, 366
SfaNI GCATC 5 cut(s) 997, 1300, 1555, 1657, 2500
SfcI CTRYAG 1 cut(s) 2262
Sfr303I CCGCGG 1 cut(s) 306
SgrBI CCGCGG 1 cut(s) 306
SinI GGWCC 3 cut(s) 12, 38, 57
SmiMI CAYNNNNRTG 2 cut(s) 1445, 1524
SmlI CTYRAG 1 cut(s) 1159
SmoI CTYRAG 1 cut(s) 1159
SphI GCATGC 1 cut(s) 865
SseBI AGGCCT 1 cut(s) 1002
SsiI CCGC 7 cut(s) 93, 303, 305, 467, 1105, 1537, 1931
SspMI CTAG 5 cut(s) 1187, 1503, 2082, 2186, 2552
StuI AGGCCT 1 cut(s) 1002
StyD4I CCNGG 5 cut(s) 53, 1110, 1556, 2346, 2541
TaaI ACNGT 3 cut(s) 952, 2272, 2525
TaiI ACGT 5 cut(s) 270, 329, 369, 1445, 2594
TaqI TCGA 3 cut(s) 213, 284, 1810
TatI WGTACW 1 cut(s) 796
TauI GCSGC 2 cut(s) 305, 469
TfiI GAWTC 8 cut(s) 132, 215, 620, 805, 1207, 1886, 2017, 2642
Tru1I TTAA 7 cut(s) 168, 636, 872, 975, 1523, 1989, 2594
Tru9I TTAA 7 cut(s) 168, 636, 872, 975, 1523, 1989, 2594
TscAI CASTG 4 cut(s) 206, 685, 1781, 2275
TseFI GTSAC 3 cut(s) 95, 2266, 2309
TseI GCWGC 9 cut(s) 563, 566, 779, 1298, 1864, 2042, 2261, 2408, 2462
Tsp45I GTSAC 3 cut(s) 95, 2266, 2309
TspDTI ATGAA 8 cut(s) 842, 1208, 1356, 1767, 2015, 2085, 2091, 2418
TspRI CASTG 4 cut(s) 206, 685, 1781, 2275
Tth111I GACNNNGTC 2 cut(s) 374, 2065
Van91I CCANNNNNTGG 1 cut(s) 701
VpaK11BI GGWCC 3 cut(s) 12, 38, 57
VspI ATTAAT 1 cut(s) 636
XagI CCTNNNNNAGG 1 cut(s) 1721
XceI RCATGY 1 cut(s) 865
XmiI GTMKAC 1 cut(s) 2164
XspI CTAG 5 cut(s) 1187, 1503, 2082, 2186, 2552
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.