MD05G1316600.v1.1

Wings apart-like protein regulation of heterochromatin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0009354
Physical Location & Seq
Forward (+)
44576638 .. 44582779
6142 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1316600.v1.1.491

Sequence Viewer

Length: 2706 bp
ATGATCGTGAGAACGTACGGCCGTCGCAAGGGAGGGGACATTACCAGAACGTATTCCGACACAGAGCTAAACGACGCCGTTCACGACGACGACGACGTCAGTAGCGACCCCTTCGGATTTTCCCTATCGCAGCCTCAAGAAAGCAGCGGTGACCCTTTCAATTTCTCGTCTCAGGAGGACTCGTCTTCCGGGTGGGCCCACTTCGATTCGGACCCTTACGTCACCAAGGACTCCTCCTTGAAGCGCTTTCCCCTGGACGGCGTCGTCTCGGGGAGGTCCAAGAAAGCTAAGACTCGGAAGGAGGCGGTGGGGAAGAACTCCTACAGGCCGCCGCCGACGCCGTCTATTCTTGCGACGTCGACCTTGATGGAGGCGCAGGAGTTTGGGGAGATGATGGAGCACGTGGACGAGGTCAATTTCGCGCTGGATGGACTCCGGAGGGGTCAGCCGGTTCGGATCAGAAGGGCTAGCTTGCTGTCGTTGCTGTCTATTTGCGGCACGGCGCAGCAGAGACGGCTTCTCAGGACTCAGGGGATGGCAAAAACTATAATCGAGGCTATATTGGGTCTCAGCTTTGATGATTCACCCAGCAATCTGGCTGCTGCAACTATTTTCTATGTTTTGACAAGTGATGGTCAGGATGATCATCTCCTGGAATCACCGAACTGTATTAATTTTTTGATTAGGTTTTGCAAACCAATTGTCTCAAACACCACAGAAGATAAAGTACCAAAAGTTGGACGTAAGCTTCTAGCATTGCGGATTGGTGCTGACATCTCGCAGTGTACGGCAAAAAGATTGGACTCCAGCTCTGCCGCTATTTTTTCCAAAGTTCAAGAAATTCTTGTAGGTTGCAAGGAATTGAAACCGAGTTGTGCGGATGAAGGAGAAATGGAAAATCCGGAGTTATGCCCAAAATGGATAGCTTTGCTGACTATGGAGAAAGCTTGCTTATCTACCATCTCTCTTGAAGAAACCACTGGTGCAGTAAGGAAGTCGGGGGGCAACTTTAAGGAAAAATTACGAGAGCTTGGAGGTCTTGATGCTGTATTTGAGGTGACCATGGGTTGCCATTCCAATATGGAGGGTTGGCTAAAAGATAGCTCGCATACTATTTGGGAAAATGAAAACGACATGGTGCGGAGCCTGGTTCTGCTTTTGAAATGTTTGAAGATCATGGAAAATGCCACATTCCTAAGTAAAGAGAATCAGAGCCATTTGCTAGGAATGAAAAGGAAGTTGGATCCCATGGCAAATCCTTTGTCTTTTACAGAACTAGTTATAAGTGCCATCAATATTCTGTCAGGTCTTTGTCTACATAAAAGTTCCTCTTCTGCCTCCAATGACAGAAAGTCTTATTGTCTATCTAATGGGAGTGAACATTTTTCTGAGAAGAGCTCCAATAGATGTCAGAGCAGTCAGTTAATGTCAACACGTCGCTCAGTATATACTATATCCAGTTCGGAAACTACCAGCACCTCCATGACTGATACTTATTCATTGAACACAAGACTCAATTCTTCTAGAAATGGTTCTTCCAATGGTGCATCAAGACATGTCAGCGGTGGAACCAGTAATTTTAGCAATCTTTCCCTGAAGAATGCTGGTCTCAGTCAGCGATCCTATAACTTTGAAGACTCCAAAATTGATCTTTCAGAGGAGAGTCAAGATCCTTTTGCATTTATTGATGGTTCCAGGATGGATGCTGATCTCAGTCAGAGATCCTATATTTTTGAAGACACCAAAATTGATCTTTCACGGGAGCGTCAAGATCCTTTTGCATTTGATGAAGATGATTATAAACCTTCTAAGTGGGACATGTTATCTGGCAAGACAAATATGTCTCTATCACAACAGAATGCGGCAGCATACAGAGAAATTGATGACATTTGTCAGTTGCAGCATATTATGAGCCAAGAAGCATCAAGTAATGGAGAAAACCATCAGACACAAGAGACATCCTGTTCAGGTGCTGTTAGCCGAGAAGGTTCCAGTCTTGTAGCTGACTGCCTTCTTACTGCTGTCAAGGTTCTGATGAACTTAGCAAATGATAATCCTGTTGGGTGTCAGCAAATTGCTGCATATGGAGGACTGGAAACCTTGTCTTCTTTGATTGCCAACCACTTTCCTTGTTTCAACTCATCATCATCTCCCTTCAGCGAGAGAAGTGAGAATATTTCCAGCATTGAACTTGACCATCCGAATAATAGACATCTTACTGATCAAGAGCTGGACTTTCTTGTAGCTATTTTGGGTCTGCTTGTGAACTTGGTGGAGAAAGATGGCCAAAACAGATCACGGCTTGCAGCAGCTAGCGTTCAAGTAGCTAGTTCGGAAGGGTTTGGAGAGGAGAGTCGCAAGGATCTAATTCTGCTAATTTGCTCTATCTTTCTTGCGAACCAAGGTGCAGGCGAGGGAGGTGGAGAAGAAATGGTTTTGCCAACGGATGAGGCAGCTGTCTTACAAGGAGAACAGGAAGCGGAGAAAATGATAATAGAAGCTTATTCTGCCCTGCTTCTAGCATTTCTTTCAACCGAAAGTAAGAGCATACGCGATGCAATTTCTGACTGTCTTCCAGACCGCAACCTGGCTATTCTAGTACCTGTGTTGGACAGATTCGTGGCCTTTCATTTGACGCTTAACATGATATCGCCGGAGACTCATAAAGCTGTGAGTGAAGTAATCGAATCATGTAGGATACGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WAPL

Protein Analysis

902

Amino Acids

98.88

Weight (kDa)

5.09

Isoelectric Point (pI)

51.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WAPL PF07814 120 - 463 7.7e-45 Wings apart-like protein regulation of heterochromatin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1283, 1800
AasI GACNNNNNNGTC 4 cut(s) 95, 218, 263, 1840
AatII GACGTC 2 cut(s) 99, 359
AccB7I CCANNNNNTGG 1 cut(s) 737
AccI GTMKAC 2 cut(s) 359, 1315
AccII CGCG 2 cut(s) 422, 2553
AccIII TCCGGA 2 cut(s) 435, 901
AclWI GGATC 8 cut(s) 464, 1238, 1251, 1614, 1664, 1716, 1766, 2370
AcoI YGGCCR 2 cut(s) 19, 2284
AcsI RAATTY 1 cut(s) 840
AcuI CTGAAG 2 cut(s) 1616, 2140
AcvI CACGTG 1 cut(s) 403
AcyI GRCGYC 5 cut(s) 75, 96, 261, 338, 356
AfaI GTAC 4 cut(s) 17, 729, 787, 2601
AfeI AGCGCT 1 cut(s) 245
AfiI CCNNNNNNNGG 4 cut(s) 28, 257, 737, 2587
AflIII ACRYGT 3 cut(s) 1433, 1555, 1818
AhdI GACNNNNNGTC 1 cut(s) 1351
AhlI ACTAGT 1 cut(s) 1276
AjiI CACGTC 1 cut(s) 1436
AjnI CCWGG 5 cut(s) 252, 651, 1146, 1694, 2586
AjuI GAANNNNNNNTTGG 2 cut(s) 1223, 1255
Alw21I GWGCWC 2 cut(s) 402, 1400
Alw26I GTCTC 9 cut(s) 174, 271, 505, 572, 709, 1613, 1848, 1949, 2651
AlwI GGATC 8 cut(s) 464, 1238, 1251, 1614, 1664, 1716, 1766, 2370
AlwNI CAGNNNCTG 1 cut(s) 1973
Ama87I CYCGRG 1 cut(s) 268
Aor13HI TCCGGA 2 cut(s) 435, 901
Aor51HI AGCGCT 1 cut(s) 245
AoxI GGCC 5 cut(s) 19, 195, 326, 2284, 2622
ApaI GGGCCC 1 cut(s) 199
ApoI RAATTY 1 cut(s) 840
ArsI GACNNNNNNTTYG 4 cut(s) 687, 719, 1247, 1279
AseI ATTAAT 1 cut(s) 672
Asp700I GAANNNNTTC 2 cut(s) 52, 1531
AspLEI GCGC 4 cut(s) 246, 376, 424, 505
AspS9I GGNCC 4 cut(s) 195, 196, 211, 276
AsuC2I CCSGG 1 cut(s) 190
AsuHPI GGTGA 5 cut(s) 161, 214, 576, 651, 1069
AsuNHI GCTAGC 2 cut(s) 467, 2312
AvaI CYCGRG 1 cut(s) 268
AvaII GGWCC 2 cut(s) 211, 276
BaeGI GKGCMC 1 cut(s) 199
BalI TGGCCA 1 cut(s) 2286
BamHI GGATCC 1 cut(s) 1243
BanII GRGCYC 2 cut(s) 199, 1400
BarI GAAGNNNNNNTAC 4 cut(s) 305, 337, 711, 743
BbrPI CACGTG 1 cut(s) 403
BbsI GAAGAC 5 cut(s) 177, 1641, 1743, 2097, 2564
Bbv12I GWGCWC 2 cut(s) 402, 1400
BceAI ACGGC 9 cut(s) 6, 34, 62, 274, 325, 516, 530, 804, 2315
BciT130I CCWGG 5 cut(s) 254, 653, 1148, 1696, 2588
BciVI GTATCC 1 cut(s) 2691
BclI TGATCA 2 cut(s) 643, 2221
BcnI CCSGG 1 cut(s) 190
BcoDI GTCTC 9 cut(s) 174, 271, 505, 572, 709, 1613, 1848, 1949, 2651
BcuI ACTAGT 1 cut(s) 1276
BfaI CTAG 9 cut(s) 468, 752, 1223, 1277, 1524, 2313, 2328, 2519, 2597
BfmI CTRYAG 1 cut(s) 322
BfoI RGCGCY 1 cut(s) 247
BfuI GTATCC 1 cut(s) 2691
Bme1390I CCNGG 6 cut(s) 190, 254, 653, 1148, 1696, 2588
Bme18I GGWCC 2 cut(s) 211, 276
BmeRI GACNNNNNGTC 1 cut(s) 1351
BmeT110I CYCGRG 1 cut(s) 268
BmgBI CACGTC 1 cut(s) 1436
BmgT120I GGNCC 4 cut(s) 195, 196, 211, 276
BmiI GGNNCC 7 cut(s) 197, 213, 1145, 1245, 1570, 1693, 1990
BmrFI CCNGG 6 cut(s) 190, 254, 653, 1148, 1696, 2588
BmsI GCATC 5 cut(s) 1033, 1556, 1693, 1931, 2545
BmtI GCTAGC 2 cut(s) 471, 2316
BoxI GACNNNNGTC 1 cut(s) 1890
BpiI GAAGAC 5 cut(s) 177, 1641, 1743, 2097, 2564
BplI GAGNNNNNCTC 2 cut(s) 1382, 1414
BpmI CTGGAG 1 cut(s) 790
BpuEI CTTGAG 1 cut(s) 120
BpuMI CCSGG 1 cut(s) 190
BsaAI YACGTR 1 cut(s) 403
BsaBI GATNNNNATC 2 cut(s) 645, 1707
BsaHI GRCGYC 5 cut(s) 75, 96, 261, 338, 356
BsaI GGTCTC 2 cut(s) 572, 1613
BsaJI CCNNGG 5 cut(s) 225, 252, 1062, 1248, 2401
BsaWI WCCGGW 2 cut(s) 435, 901
BsaXI ACNNNNNCTCC 2 cut(s) 167, 197
Bsc4I CCNNNNNNNGG 4 cut(s) 28, 257, 737, 2587
Bse118I RCCGGY 1 cut(s) 448
Bse1I ACTGG 5 cut(s) 985, 1458, 1572, 1992, 2097
Bse3DI GCAATG 1 cut(s) 755
Bse8I GATNNNNATC 2 cut(s) 645, 1707
BseAI TCCGGA 2 cut(s) 435, 901
BseBI CCWGG 5 cut(s) 254, 653, 1148, 1696, 2588
BseDI CCNNGG 5 cut(s) 225, 252, 1062, 1248, 2401
BseGI GGATG 9 cut(s) 433, 540, 646, 886, 1704, 1708, 1958, 2197, 2452
BseJI GATNNNNATC 2 cut(s) 645, 1707
BseLI CCNNNNNNNGG 4 cut(s) 28, 257, 737, 2587
BseMI GCAATG 1 cut(s) 755
BseMII CTCAG 8 cut(s) 185, 535, 542, 583, 1380, 1455, 1624, 1726
BseNI ACTGG 5 cut(s) 985, 1458, 1572, 1992, 2097
BseRI GAGGAG 3 cut(s) 223, 1673, 2363
BseSI GKGCMC 1 cut(s) 199
BseX3I CGGCCG 1 cut(s) 19
BseYI CCCAGC 1 cut(s) 587
BsgI GTGCAG 2 cut(s) 1005, 2427
Bsh1236I CGCG 2 cut(s) 422, 2553
Bsh1285I CGRYCG 1 cut(s) 22
BshFI GGCC 5 cut(s) 21, 197, 328, 2286, 2624
BsiEI CGRYCG 1 cut(s) 22
BsiHKAI GWGCWC 2 cut(s) 402, 1400
BsiHKCI CYCGRG 1 cut(s) 268
BsiSI CCGG 5 cut(s) 189, 436, 449, 902, 2654
BsiWI CGTACG 1 cut(s) 15
BslFI GGGAC 2 cut(s) 50, 1829
BslI CCNNNNNNNGG 4 cut(s) 28, 257, 737, 2587
BsmAI GTCTC 9 cut(s) 174, 271, 505, 572, 709, 1613, 1848, 1949, 2651
BsmBI CGTCTC 3 cut(s) 174, 271, 505
BsmFI GGGAC 2 cut(s) 50, 1829
BsmI GAATGC 2 cut(s) 1606, 1864
BsnI GGCC 5 cut(s) 21, 197, 328, 2286, 2624
Bso31I GGTCTC 2 cut(s) 572, 1613
BsoBI CYCGRG 1 cut(s) 268
Bsp120I GGGCCC 1 cut(s) 195
Bsp1286I GDGCHC 3 cut(s) 199, 402, 1400
Bsp13I TCCGGA 2 cut(s) 435, 901
Bsp19I CCATGG 2 cut(s) 1062, 1248
BspANI GGCC 5 cut(s) 21, 197, 328, 2286, 2624
BspCNI CTCAG 8 cut(s) 184, 534, 541, 582, 1381, 1454, 1623, 1725
BspEI TCCGGA 2 cut(s) 435, 901
BspFNI CGCG 2 cut(s) 422, 2553
BspLI GGNNCC 7 cut(s) 197, 213, 1145, 1245, 1570, 1693, 1990
BspOI GCTAGC 2 cut(s) 471, 2316
BspPI GGATC 8 cut(s) 464, 1238, 1251, 1614, 1664, 1716, 1766, 2370
BspQI GCTCTTC 1 cut(s) 1388
BspTNI GGTCTC 2 cut(s) 572, 1613
BsrDI GCAATG 1 cut(s) 755
BsrFI RCCGGY 1 cut(s) 448
BsrI ACTGG 5 cut(s) 985, 1458, 1572, 1992, 2097
BssAI RCCGGY 1 cut(s) 448
BssECI CCNNGG 5 cut(s) 225, 252, 1062, 1248, 2401
BssNI GRCGYC 5 cut(s) 75, 96, 261, 338, 356
BssT1I CCWWGG 4 cut(s) 225, 1062, 1248, 2401
Bst2UI CCWGG 5 cut(s) 254, 653, 1148, 1696, 2588
Bst4CI ACNGT 3 cut(s) 668, 2570, 2703
Bst6I CTCTTC 2 cut(s) 1336, 1388
BstACI GRCGYC 5 cut(s) 75, 96, 261, 338, 356
BstBAI YACGTR 1 cut(s) 403
BstC8I GCNNGC 7 cut(s) 469, 473, 949, 1106, 2304, 2314, 2410
BstDSI CCRYGG 2 cut(s) 1062, 1248
BstEII GGTNACC 2 cut(s) 149, 1057
BstF5I GGATG 9 cut(s) 433, 540, 646, 886, 1704, 1708, 1958, 2197, 2452
BstFNI CGCG 2 cut(s) 422, 2553
BstH2I RGCGCY 1 cut(s) 247
BstHHI GCGC 4 cut(s) 246, 376, 424, 505
BstMAI GTCTC 9 cut(s) 174, 271, 505, 572, 709, 1613, 1848, 1949, 2651
BstMCI CGRYCG 1 cut(s) 22
BstMWI GCNNNNNNNGC 4 cut(s) 337, 481, 514, 2507
BstNI CCWGG 5 cut(s) 254, 653, 1148, 1696, 2588
BstNSI RCATGY 2 cut(s) 1559, 1822
BstPAI GACNNNNGTC 1 cut(s) 1890
BstPI GGTNACC 2 cut(s) 149, 1057
BstSCI CCNGG 6 cut(s) 188, 252, 651, 1146, 1694, 2586
BstSFI CTRYAG 1 cut(s) 322
BstSLI GKGCMC 1 cut(s) 199
BstUI CGCG 2 cut(s) 422, 2553
BstV2I GAAGAC 5 cut(s) 177, 1641, 1743, 2097, 2564
BstX2I RGATCY 5 cut(s) 1243, 1669, 1721, 1771, 2362
BstXI CCANNNNNNTGG 1 cut(s) 595
BstYI RGATCY 5 cut(s) 1243, 1669, 1721, 1771, 2362
BstZI CGGCCG 1 cut(s) 19
BsuI GTATCC 1 cut(s) 2691
BsuRI GGCC 5 cut(s) 21, 197, 328, 2286, 2624
BtgI CCRYGG 2 cut(s) 1062, 1248
BtgZI GCGATG 1 cut(s) 2568
BtrI CACGTC 1 cut(s) 1436
BtsCI GGATG 9 cut(s) 433, 540, 646, 886, 1704, 1708, 1958, 2197, 2452
BtsI GCAGTG 1 cut(s) 788
BtsIMutI CAGTG 2 cut(s) 788, 978
Cac8I GCNNGC 7 cut(s) 469, 473, 949, 1106, 2304, 2314, 2410
CaiI CAGNNNCTG 1 cut(s) 1973
CfoI GCGC 4 cut(s) 246, 376, 424, 505
Cfr10I RCCGGY 1 cut(s) 448
Cfr13I GGNCC 4 cut(s) 195, 196, 211, 276
CseI GACGC 5 cut(s) 83, 250, 346, 1754, 2644
Csp6I GTAC 4 cut(s) 16, 728, 786, 2600
CspCI CAANNNNNGTGG 2 cut(s) 2111, 2146
CviAII CATG 9 cut(s) 1063, 1135, 1177, 1249, 1483, 1556, 1819, 2644, 2691
CviQI GTAC 4 cut(s) 16, 728, 786, 2600
DrdI GACNNNNNNGTC 4 cut(s) 95, 218, 263, 1840
DriI GACNNNNNGTC 1 cut(s) 1351
DseDI GACNNNNNNGTC 4 cut(s) 95, 218, 263, 1840
EaeI YGGCCR 2 cut(s) 19, 2284
EagI CGGCCG 1 cut(s) 19
Eam1104I CTCTTC 2 cut(s) 1336, 1388
Eam1105I GACNNNNNGTC 1 cut(s) 1351
EarI CTCTTC 2 cut(s) 1336, 1388
Ecl136II GAGCTC 1 cut(s) 1398
EclXI CGGCCG 1 cut(s) 19
Eco130I CCWWGG 4 cut(s) 225, 1062, 1248, 2401
Eco24I GRGCYC 2 cut(s) 199, 1400
Eco31I GGTCTC 2 cut(s) 572, 1613
Eco32I GATATC 1 cut(s) 2649
Eco47I GGWCC 2 cut(s) 211, 276
Eco47III AGCGCT 1 cut(s) 245
Eco52I CGGCCG 1 cut(s) 19
Eco53kI GAGCTC 1 cut(s) 1398
Eco57I CTGAAG 2 cut(s) 1616, 2140
Eco72I CACGTG 1 cut(s) 403
Eco88I CYCGRG 1 cut(s) 268
Eco91I GGTNACC 2 cut(s) 149, 1057
EcoICRI GAGCTC 1 cut(s) 1398
EcoO65I GGTNACC 2 cut(s) 149, 1057
EcoRII CCWGG 5 cut(s) 252, 651, 1146, 1694, 2586
EcoRV GATATC 1 cut(s) 2649
EcoT14I CCWWGG 4 cut(s) 225, 1062, 1248, 2401
EcoT38I GRGCYC 2 cut(s) 199, 1400
ErhI CCWWGG 4 cut(s) 225, 1062, 1248, 2401
Esp3I CGTCTC 3 cut(s) 174, 271, 505
FaeI CATG 9 cut(s) 1066, 1138, 1180, 1252, 1486, 1559, 1822, 2647, 2694
FalI AAGNNNNNCTT 4 cut(s) 828, 860, 1315, 1347
FaqI GGGAC 2 cut(s) 50, 1829
FatI CATG 9 cut(s) 1062, 1134, 1176, 1248, 1482, 1555, 1818, 2643, 2690
FauNDI CATATG 1 cut(s) 2083
FbaI TGATCA 2 cut(s) 643, 2221
FblI GTMKAC 2 cut(s) 359, 1315
FokI GGATG 9 cut(s) 440, 547, 653, 893, 1711, 1715, 1945, 2184, 2459
FriOI GRGCYC 2 cut(s) 199, 1400
FspBI CTAG 9 cut(s) 468, 752, 1223, 1277, 1524, 2313, 2328, 2519, 2597
GlaI GCGC 4 cut(s) 245, 375, 423, 504
GsaI CCCAGC 1 cut(s) 591
GsuI CTGGAG 1 cut(s) 790
HaeII RGCGCY 1 cut(s) 247
HaeIII GGCC 5 cut(s) 21, 197, 328, 2286, 2624
HapII CCGG 5 cut(s) 189, 436, 449, 902, 2654
HgaI GACGC 5 cut(s) 83, 250, 346, 1754, 2644
HhaI GCGC 4 cut(s) 246, 376, 424, 505
Hin1I GRCGYC 5 cut(s) 75, 96, 261, 338, 356
Hin1II CATG 9 cut(s) 1066, 1138, 1180, 1252, 1486, 1559, 1822, 2647, 2694
Hin6I GCGC 4 cut(s) 244, 374, 422, 503
HinP1I GCGC 4 cut(s) 244, 374, 422, 503
HincII GTYRAC 2 cut(s) 360, 1431
HindII GTYRAC 2 cut(s) 360, 1431
HindIII AAGCTT 3 cut(s) 746, 945, 2499
HpaII CCGG 5 cut(s) 189, 436, 449, 902, 2654
HphI GGTGA 5 cut(s) 161, 214, 576, 651, 1069
Hpy166II GTNNAC 8 cut(s) 82, 360, 406, 786, 1316, 1379, 1431, 2266
Hpy8I GTNNAC 8 cut(s) 82, 360, 406, 786, 1316, 1379, 1431, 2266
HpyAV CCTTC 9 cut(s) 121, 292, 456, 878, 1815, 1979, 2021, 2164, 2330
HpyCH4III ACNGT 3 cut(s) 668, 2570, 2703
HpyCH4IV ACGT 8 cut(s) 14, 50, 96, 219, 356, 402, 742, 1435
HpyF10VI GCNNNNNNNGC 4 cut(s) 337, 481, 514, 2507
HpySE526I ACGT 8 cut(s) 14, 50, 96, 219, 356, 402, 742, 1435
Hsp92I GRCGYC 5 cut(s) 75, 96, 261, 338, 356
Hsp92II CATG 9 cut(s) 1066, 1138, 1180, 1252, 1486, 1559, 1822, 2647, 2694
HspAI GCGC 4 cut(s) 244, 374, 422, 503
Kpn2I TCCGGA 2 cut(s) 435, 901
Ksp22I TGATCA 2 cut(s) 643, 2221
LguI GCTCTTC 1 cut(s) 1388
LmnI GCTCC 4 cut(s) 397, 1143, 1403, 1762
LweI GCATC 5 cut(s) 1033, 1556, 1693, 1931, 2545
MaeI CTAG 9 cut(s) 468, 752, 1223, 1277, 1524, 2313, 2328, 2519, 2597
MaeII ACGT 8 cut(s) 14, 50, 96, 219, 356, 402, 742, 1435
MaeIII GTNAC 3 cut(s) 149, 220, 1057
MfeI CAATTG 1 cut(s) 699
MflI RGATCY 5 cut(s) 1243, 1669, 1721, 1771, 2362
MhlI GDGCHC 3 cut(s) 199, 402, 1400
MlsI TGGCCA 1 cut(s) 2286
MluNI TGGCCA 1 cut(s) 2286
MmeI TCCRAC 4 cut(s) 81, 718, 1221, 2589
Mox20I TGGCCA 1 cut(s) 2286
MroI TCCGGA 2 cut(s) 435, 901
MroXI GAANNNNTTC 2 cut(s) 52, 1531
MscI TGGCCA 1 cut(s) 2286
MseI TTAA 4 cut(s) 672, 1011, 1424, 2640
MslI CAYNNNNRTG 1 cut(s) 1481
Msp20I TGGCCA 1 cut(s) 2286
MspA1I CMGCKG 3 cut(s) 147, 1563, 2456
MspI CCGG 5 cut(s) 189, 436, 449, 902, 2654
MspR9I CCNGG 6 cut(s) 190, 254, 653, 1148, 1696, 2588
MunI CAATTG 1 cut(s) 699
Mva1269I GAATGC 2 cut(s) 1606, 1864
MvaI CCWGG 5 cut(s) 254, 653, 1148, 1696, 2588
MvnI CGCG 2 cut(s) 422, 2553
MwoI GCNNNNNNNGC 4 cut(s) 337, 481, 514, 2507
NciI CCSGG 1 cut(s) 190
NcoI CCATGG 2 cut(s) 1062, 1248
NdeI CATATG 1 cut(s) 2083
NheI GCTAGC 2 cut(s) 467, 2312
NlaIII CATG 9 cut(s) 1066, 1138, 1180, 1252, 1486, 1559, 1822, 2647, 2694
NlaIV GGNNCC 7 cut(s) 197, 213, 1145, 1245, 1570, 1693, 1990
NmeAIII GCCGAG 1 cut(s) 2006
NmuCI GTSAC 3 cut(s) 149, 220, 1057
NspI RCATGY 2 cut(s) 1559, 1822
PciI ACATGT 2 cut(s) 1555, 1818
PciSI GCTCTTC 1 cut(s) 1388
PcsI WCGNNNNNNNCGW 4 cut(s) 81, 90, 93, 102
PctI GAATGC 2 cut(s) 1606, 1864
PdmI GAANNNNTTC 2 cut(s) 52, 1531
PfeI GAWTC 6 cut(s) 206, 581, 656, 1207, 2616, 2687
Pfl23II CGTACG 1 cut(s) 15
PflFI GACNNNGTC 4 cut(s) 95, 260, 340, 410
PflMI CCANNNNNTGG 1 cut(s) 737
PfoI TCCNGGA 2 cut(s) 651, 1694
PmaCI CACGTG 1 cut(s) 403
PmlI CACGTG 1 cut(s) 403
Ppu21I YACGTR 1 cut(s) 403
PscI ACATGT 2 cut(s) 1555, 1818
PshAI GACNNNNGTC 1 cut(s) 1890
PshBI ATTAAT 1 cut(s) 672
PsiI TTATAA 2 cut(s) 1283, 1800
Psp124BI GAGCTC 1 cut(s) 1400
Psp6I CCWGG 5 cut(s) 252, 651, 1146, 1694, 2586
PspCI CACGTG 1 cut(s) 403
PspEI GGTNACC 2 cut(s) 149, 1057
PspFI CCCAGC 1 cut(s) 587
PspGI CCWGG 5 cut(s) 252, 651, 1146, 1694, 2586
PspLI CGTACG 1 cut(s) 15
PspN4I GGNNCC 7 cut(s) 197, 213, 1145, 1245, 1570, 1693, 1990
PspOMI GGGCCC 1 cut(s) 195
PspPI GGNCC 4 cut(s) 195, 196, 211, 276
PstNI CAGNNNCTG 1 cut(s) 1973
PsuI RGATCY 5 cut(s) 1243, 1669, 1721, 1771, 2362
PsyI GACNNNGTC 4 cut(s) 95, 260, 340, 410
PvuII CAGCTG 1 cut(s) 2456
RsaI GTAC 4 cut(s) 17, 729, 787, 2601
RsaNI GTAC 4 cut(s) 16, 728, 786, 2600
RseI CAYNNNNRTG 1 cut(s) 1481
SacI GAGCTC 1 cut(s) 1400
SalI GTCGAC 1 cut(s) 358
SapI GCTCTTC 1 cut(s) 1388
SaqAI TTAA 4 cut(s) 672, 1011, 1424, 2640
Sau96I GGNCC 4 cut(s) 195, 196, 211, 276
ScrFI CCNGG 6 cut(s) 190, 254, 653, 1148, 1696, 2588
SduI GDGCHC 3 cut(s) 199, 402, 1400
SfaNI GCATC 5 cut(s) 1033, 1556, 1693, 1931, 2545
SfcI CTRYAG 1 cut(s) 322
SinI GGWCC 2 cut(s) 211, 276
SmiMI CAYNNNNRTG 1 cut(s) 1481
SmlI CTYRAG 1 cut(s) 135
SmoI CTYRAG 1 cut(s) 135
SpeI ACTAGT 1 cut(s) 1276
SspI AATATT 2 cut(s) 1297, 2176
SspMI CTAG 9 cut(s) 468, 752, 1223, 1277, 1524, 2313, 2328, 2519, 2597
SstI GAGCTC 1 cut(s) 1400
StyD4I CCNGG 6 cut(s) 188, 252, 651, 1146, 1694, 2586
StyI CCWWGG 4 cut(s) 225, 1062, 1248, 2401
TaaI ACNGT 3 cut(s) 668, 2570, 2703
TaiI ACGT 8 cut(s) 17, 53, 99, 222, 359, 405, 745, 1438
TaqI TCGA 4 cut(s) 204, 359, 552, 2685
TauI GCSGC 5 cut(s) 331, 334, 498, 818, 1865
TfiI GAWTC 6 cut(s) 206, 581, 656, 1207, 2616, 2687
Tru1I TTAA 4 cut(s) 672, 1011, 1424, 2640
Tru9I TTAA 4 cut(s) 672, 1011, 1424, 2640
TscAI CASTG 2 cut(s) 788, 985
TseFI GTSAC 3 cut(s) 149, 220, 1057
Tsp45I GTSAC 3 cut(s) 149, 220, 1057
TspDTI ATGAA 7 cut(s) 897, 1140, 1244, 1488, 1803, 2051, 2618
TspGWI ACGGA 1 cut(s) 2459
TspRI CASTG 2 cut(s) 788, 985
Tth111I GACNNNGTC 4 cut(s) 95, 260, 340, 410
Van91I CCANNNNNTGG 1 cut(s) 737
VpaK11BI GGWCC 2 cut(s) 211, 276
VspI ATTAAT 1 cut(s) 672
XapI RAATTY 1 cut(s) 840
XbaI TCTAGA 1 cut(s) 1523
XceI RCATGY 2 cut(s) 1559, 1822
XmiI GTMKAC 2 cut(s) 359, 1315
XmnI GAANNNNTTC 2 cut(s) 52, 1531
XspI CTAG 9 cut(s) 468, 752, 1223, 1277, 1524, 2313, 2328, 2519, 2597
ZraI GACGTC 2 cut(s) 97, 357
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.