MD10G1297100.v1.1

Wings apart-like protein regulation of heterochromatin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0009354
Physical Location & Seq
Forward (+)
38466834 .. 38473654
6821 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1297100.v1.1.491

Sequence Viewer

Length: 2703 bp
ATGATCGTGAGAACGTACGGCCGTCGCAAGGGAGGGGGCATTCCCAGTACTTATTCCGACACAGAGCTAAACGACGCCGTTCACGACGACGACGATGTCGGCAGCGATCCCTTCGGATTTTCCCTGTCGCAGCCGCAAGAAAGCAGCGACCCTTTCAATTTCTCGTCTCAGGAGGACTCGTCTTCCGGGTGGGCCCATTTCGATTCGGACCCCTACGTCACCAAGGACGACTCCTTGAAGCGCTTTCCGCTGGACGGCGTCGTCGCGGGGCGTTCCAAGAAAGCCAAGACTCGGAAGGAGGTGGTGGGAAAGAACTCCTGCAGGCCGCCACTGCCGCCGTCAATTCTTGCGACTTCGACTTTGATGGAGGCCCAGGAGTTTGGAGAGATGATGGAGCACGTGGACGAGGTCAATTTCGCTTTGGATGGGCTCCGGAAGGGTCAGCCGGTTCGGATCAGAAGGGCTAGCTTGCTCTCATTGCTGTCTATTTGTGGGACGGCGCAGCAGAGGCGGCTTCTCAGGACTCAGGGGATGGCAAAAACAATAATAGAGGCTATAATGGGTCTCAGCTTTGATGATTCACCCAGCAATCTGGCTGCTGCAACTATTTTCTATGTTTTGACAAGTGATGGTCAGGATGATCATCTTCTGGAATCACCAAACTGTATTAATTTTTTGATTAGGTTTTGCAAACCAATTGTCTCAAACACCACTGAAGATAAAGTACCAAAAATTGGACGTAAGCTTCTAGCATTGCGTATCGGTGCTGACATCTCTCAGTGTACGACAAAAAGATTGGACTCCAGCTCTGCCGCTATCTTTTCCAAAGTTCAAGAAATTCTTGTAGGTTGCAAGGACTTGAAGCCGAGTTGTGCGGATGATGGAGGAATGGAAAAGCCGGAGTTATGCCCAAAATGGATAGCTTTGCTGACTATTGAGAAAGCTTGCTTATCTACTATTTCTCTTGAAGAAACCACTGGCGCAGTAAGGAAGTTGGGGTGCAACTTTAAGGAAAAATTACGAGAGCTTGGAGGTCTTGATGCTGTATTTGAGGTGACCATGGGTTGCCATTCCAATATGGAGGGTTGGCTAAAAGATAGCTCACATTCTATTTGGGAAAAGGAAATCGACATGGTGCGGAGCCTGGTTCTTCTTTTGAAATGTTTGAAGATCATGGAAAATGCGACATTCCTAAGTAAAGAGAATCAGAGCCATTTGCTAGAAATGAAAAGGAAGTTGGATCCCATGGCAATTCCTATGTCTTTTACAGAACTTGTTATAAGTGCCATCAATATTCTATCAGGGCTTTATCTACAGGAAAGTTCCTCATCTGCCTCTAATGACGAAAAGTCTTATAGTCTCTCTAATGGGGTTGAACATGTTTCTAAGAAGAGATCTGATATATGTCAGAGCAATCAGGTAATGTCAACGCCTTGCTCAGTGTATACTATATCCAGTTCGGAAACTACCAGCACCTCCATGGCTGATGCTTATTCATTGAAGACAAGACTCAATTCTTCTAGAAATGGTTCTTCCAATGGTGTATCAAGCCATGTAAGCAGTGGAATCAGTAAATTTAGCAATCTTACCACGAAGAATGCTGGTCTCAGACGGCGATCCTCTAACTTTGACAACTCCAAAATTGATCTTTCAGAAGAGAGTCAAGATCCTTTCGCATTTAGCGAAGATTCCAGGATGGATGCTGATCTCAGTCAGAGATCCTATATTTCCCAAGACTCCAAAACTGATCTTTCACAGGAGAGTCAAGATCCTTTTGCATTTGATGAAAATGATTATAAACCTTCTAAGTGGGACAAGTTATCTGGCAAGAAAAAGTTCTCTCTTTCGCAGGAGAATGCGACAGCATACAGAGAAATTGATGACATTTGTCAATTGCAGCTTATTATGAGCCAAGAAGCATCAAGTAATGGAGAAAACCATCAGACACAAGAGACAACCTGTTCAGGTGCTGTTAGCAGAGAAGGTTCCAGTATTGTAGCTGACTGCCTTCTTACTGCTGTCAAGGTTCTGATGAACTTAGCAAATGATAATCCTGTTGGGTGTCAGCAAATTGCTGCCTATGGAGGACTGGAAACCTTGTCTTCCTTGATTGCCAACCACTTTCCATCTTTCAACTCATTATCGTCTCCCTTCAGCGAGAGAAGTGAGAATACTTCCAGCATTGAACTTGACCATCAGAATGATAAACGTCTCACTGATCAAGAGCTGGACTTTCTTGTTGCTATTTTAGGCCTGCTTGTGAACTTAGTGGAGAAAAATGGCCAAAACAGATCGCGTCTTGCAGCAGCTAGTGTTCACTTACCTAGTTCGGAAGGGTTTAAAGAGGAGAGTCGCAAGGATCTAATTCTGCTAATCTGCTCTATCTTTCTAGCCAACCAAGGTGCAGGCGAGGGAGGTGGAGAAGAAATGGTTTTACCAACTGATGAGGCAGCTGTCTTACAAGGAGAACAGGAAGCAGAGAAAATGATAGTAGAAGCTTATTCTGCCCTGCTTCTAGCGTTTCTTTCAACTGAGAGTAAGAGCATACGTGATGCAATTTCTGACTGTCTCCCAGACCGCAACCTGGCTATCCTAGTACCTGTGTTGGACAGATTTGTGGCATTCCATTTGACGCTAAACATGATATCGCCCGAGACGCATAAAGCTGTGAGCGAAGTAATTGAATCATGTAGGATACGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WAPL

Protein Analysis

901

Amino Acids

98.69

Weight (kDa)

5.12

Isoelectric Point (pI)

52.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WAPL PF07814 119 - 762 3.4e-50 Wings apart-like protein regulation of heterochromatin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1280, 1797
AasI GACNNNNNNGTC 3 cut(s) 95, 215, 260
AccB7I CCANNNNNTGG 1 cut(s) 734
AccI GTMKAC 1 cut(s) 1445
AccII CGCG 2 cut(s) 266, 2296
AccIII TCCGGA 1 cut(s) 432
AclWI GGATC 9 cut(s) 101, 461, 1235, 1248, 1611, 1661, 1713, 1763, 2367
AcoI YGGCCR 2 cut(s) 19, 2281
AcsI RAATTY 2 cut(s) 837, 1574
AcuI CTGAAG 2 cut(s) 735, 2137
AcvI CACGTG 1 cut(s) 400
AcyI GRCGYC 2 cut(s) 75, 258
AfaI GTAC 5 cut(s) 17, 49, 726, 784, 2598
AfeI AGCGCT 1 cut(s) 242
AfiI CCNNNNNNNGG 5 cut(s) 28, 254, 291, 734, 2584
AflIII ACRYGT 1 cut(s) 1378
AhdI GACNNNNNGTC 1 cut(s) 1348
AjnI CCWGG 4 cut(s) 372, 1143, 1691, 2583
AjuI GAANNNNNNNTTGG 2 cut(s) 1220, 1252
Alw21I GWGCWC 1 cut(s) 399
AlwI GGATC 9 cut(s) 101, 461, 1235, 1248, 1611, 1661, 1713, 1763, 2367
AlwNI CAGNNNCTG 1 cut(s) 1970
Ama87I CYCGRG 1 cut(s) 2651
Aor13HI TCCGGA 1 cut(s) 432
Aor51HI AGCGCT 1 cut(s) 242
AoxI GGCC 6 cut(s) 19, 192, 323, 369, 2251, 2281
ApaI GGGCCC 1 cut(s) 196
ApoI RAATTY 2 cut(s) 837, 1574
ArsI GACNNNNNNTTYG 4 cut(s) 343, 375, 684, 716
AseI ATTAAT 1 cut(s) 669
Asp700I GAANNNNTTC 2 cut(s) 1528, 1835
AspLEI GCGC 3 cut(s) 243, 502, 983
AspS9I GGNCC 4 cut(s) 192, 193, 208, 370
AsuC2I CCSGG 1 cut(s) 187
AsuHPI GGTGA 4 cut(s) 211, 573, 648, 1066
AsuNHI GCTAGC 1 cut(s) 464
AvaI CYCGRG 1 cut(s) 2651
AvaII GGWCC 1 cut(s) 208
BaeGI GKGCMC 1 cut(s) 196
BalI TGGCCA 1 cut(s) 2283
BamHI GGATCC 1 cut(s) 1240
BanII GRGCYC 2 cut(s) 196, 432
BarI GAAGNNNNNNTAC 4 cut(s) 708, 740, 2155, 2187
BbrPI CACGTG 1 cut(s) 400
BbsI GAAGAC 3 cut(s) 174, 1508, 2094
Bbv12I GWGCWC 1 cut(s) 399
BceAI ACGGC 7 cut(s) 6, 34, 62, 271, 322, 513, 1628
BciT130I CCWGG 4 cut(s) 374, 1145, 1693, 2585
BciVI GTATCC 1 cut(s) 2688
BclI TGATCA 2 cut(s) 640, 2218
BcnI CCSGG 1 cut(s) 187
BfaI CTAG 9 cut(s) 465, 749, 1220, 1521, 2310, 2325, 2390, 2516, 2594
BfmI CTRYAG 2 cut(s) 319, 1313
BfoI RGCGCY 1 cut(s) 244
BfuI GTATCC 1 cut(s) 2688
BglII AGATCT 1 cut(s) 1394
BmcAI AGTACT 1 cut(s) 49
Bme1390I CCNGG 5 cut(s) 187, 374, 1145, 1693, 2585
Bme18I GGWCC 1 cut(s) 208
BmeRI GACNNNNNGTC 1 cut(s) 1348
BmeT110I CYCGRG 1 cut(s) 2651
BmgT120I GGNCC 4 cut(s) 192, 193, 208, 370
BmiI GGNNCC 6 cut(s) 194, 210, 431, 1142, 1242, 1987
BmrFI CCNGG 5 cut(s) 187, 374, 1145, 1693, 2585
BmrI ACTGGG 1 cut(s) 39
BmsI GCATC 5 cut(s) 1030, 1477, 1690, 1928, 2542
BmtI GCTAGC 1 cut(s) 468
BmuI ACTGGG 1 cut(s) 39
BoxI GACNNNNGTC 1 cut(s) 1887
BpiI GAAGAC 3 cut(s) 174, 1508, 2094
BpmI CTGGAG 1 cut(s) 787
BpuMI CCSGG 1 cut(s) 187
BsaAI YACGTR 2 cut(s) 400, 2549
BsaBI GATNNNNATC 2 cut(s) 642, 1704
BsaHI GRCGYC 2 cut(s) 75, 258
BsaI GGTCTC 2 cut(s) 569, 1610
BsaJI CCNNGG 6 cut(s) 222, 372, 1059, 1245, 1479, 2398
BsaWI WCCGGW 1 cut(s) 432
BsaXI ACNNNNNCTCC 2 cut(s) 164, 194
Bsc4I CCNNNNNNNGG 5 cut(s) 28, 254, 291, 734, 2584
Bse118I RCCGGY 1 cut(s) 445
Bse1I ACTGG 5 cut(s) 45, 982, 1455, 1989, 2094
Bse3DI GCAATG 2 cut(s) 476, 752
Bse8I GATNNNNATC 2 cut(s) 642, 1704
BseAI TCCGGA 1 cut(s) 432
BseBI CCWGG 4 cut(s) 374, 1145, 1693, 2585
BseDI CCNNGG 6 cut(s) 222, 372, 1059, 1245, 1479, 2398
BseGI GGATG 6 cut(s) 430, 537, 643, 883, 1701, 1705
BseJI GATNNNNATC 2 cut(s) 642, 1704
BseLI CCNNNNNNNGG 5 cut(s) 28, 254, 291, 734, 2584
BseMI GCAATG 2 cut(s) 476, 752
BseMII CTCAG 9 cut(s) 182, 532, 539, 580, 791, 1452, 1621, 1723, 2523
BseNI ACTGG 5 cut(s) 45, 982, 1455, 1989, 2094
BseRI GAGGAG 1 cut(s) 2360
BseSI GKGCMC 1 cut(s) 196
BseX3I CGGCCG 1 cut(s) 19
BseYI CCCAGC 1 cut(s) 584
BsgI GTGCAG 1 cut(s) 2424
Bsh1236I CGCG 2 cut(s) 266, 2296
Bsh1285I CGRYCG 1 cut(s) 22
BshFI GGCC 6 cut(s) 21, 194, 325, 371, 2253, 2283
BsiEI CGRYCG 1 cut(s) 22
BsiHKAI GWGCWC 1 cut(s) 399
BsiHKCI CYCGRG 1 cut(s) 2651
BsiSI CCGG 4 cut(s) 186, 433, 446, 899
BsiWI CGTACG 1 cut(s) 15
BslFI GGGAC 2 cut(s) 508, 1826
BslI CCNNNNNNNGG 5 cut(s) 28, 254, 291, 734, 2584
BsmBI CGTCTC 4 cut(s) 171, 2151, 2216, 2648
BsmFI GGGAC 2 cut(s) 508, 1826
BsmI GAATGC 4 cut(s) 39, 1603, 1861, 2621
BsnI GGCC 6 cut(s) 21, 194, 325, 371, 2253, 2283
Bso31I GGTCTC 2 cut(s) 569, 1610
BsoBI CYCGRG 1 cut(s) 2651
Bsp120I GGGCCC 1 cut(s) 192
Bsp1286I GDGCHC 3 cut(s) 196, 399, 432
Bsp13I TCCGGA 1 cut(s) 432
Bsp19I CCATGG 3 cut(s) 1059, 1245, 1479
BspANI GGCC 6 cut(s) 21, 194, 325, 371, 2253, 2283
BspCNI CTCAG 9 cut(s) 181, 531, 538, 579, 790, 1451, 1620, 1722, 2524
BspEI TCCGGA 1 cut(s) 432
BspFNI CGCG 2 cut(s) 266, 2296
BspLI GGNNCC 6 cut(s) 194, 210, 431, 1142, 1242, 1987
BspMAI CTGCAG 1 cut(s) 323
BspOI GCTAGC 1 cut(s) 468
BspPI GGATC 9 cut(s) 101, 461, 1235, 1248, 1611, 1661, 1713, 1763, 2367
BspTNI GGTCTC 2 cut(s) 569, 1610
BsrDI GCAATG 2 cut(s) 476, 752
BsrFI RCCGGY 1 cut(s) 445
BsrI ACTGG 5 cut(s) 45, 982, 1455, 1989, 2094
BssAI RCCGGY 1 cut(s) 445
BssECI CCNNGG 6 cut(s) 222, 372, 1059, 1245, 1479, 2398
BssNAI GTATAC 1 cut(s) 1446
BssNI GRCGYC 2 cut(s) 75, 258
BssT1I CCWWGG 5 cut(s) 222, 1059, 1245, 1479, 2398
Bst1107I GTATAC 1 cut(s) 1446
Bst2UI CCWGG 4 cut(s) 374, 1145, 1693, 2585
Bst4CI ACNGT 3 cut(s) 665, 2567, 2700
Bst6I CTCTTC 2 cut(s) 1385, 1650
BstACI GRCGYC 2 cut(s) 75, 258
BstBAI YACGTR 2 cut(s) 400, 2549
BstC8I GCNNGC 6 cut(s) 323, 466, 470, 946, 2255, 2407
BstDSI CCRYGG 3 cut(s) 1059, 1245, 1479
BstEII GGTNACC 1 cut(s) 1054
BstF5I GGATG 6 cut(s) 430, 537, 643, 883, 1701, 1705
BstFNI CGCG 2 cut(s) 266, 2296
BstH2I RGCGCY 1 cut(s) 244
BstHHI GCGC 3 cut(s) 243, 502, 983
BstMCI CGRYCG 1 cut(s) 22
BstMWI GCNNNNNNNGC 9 cut(s) 247, 331, 334, 478, 508, 511, 1557, 2504, 2656
BstNI CCWGG 4 cut(s) 374, 1145, 1693, 2585
BstNSI RCATGY 1 cut(s) 1382
BstPAI GACNNNNGTC 1 cut(s) 1887
BstPI GGTNACC 1 cut(s) 1054
BstSCI CCNGG 5 cut(s) 185, 372, 1143, 1691, 2583
BstSFI CTRYAG 2 cut(s) 319, 1313
BstSLI GKGCMC 1 cut(s) 196
BstUI CGCG 2 cut(s) 266, 2296
BstV2I GAAGAC 3 cut(s) 174, 1508, 2094
BstX2I RGATCY 6 cut(s) 1240, 1394, 1666, 1718, 1768, 2359
BstXI CCANNNNNNTGG 2 cut(s) 380, 592
BstYI RGATCY 6 cut(s) 1240, 1394, 1666, 1718, 1768, 2359
BstZ17I GTATAC 1 cut(s) 1446
BstZI CGGCCG 1 cut(s) 19
BsuI GTATCC 1 cut(s) 2688
BsuRI GGCC 6 cut(s) 21, 194, 325, 371, 2253, 2283
BtgI CCRYGG 3 cut(s) 1059, 1245, 1479
BtsCI GGATG 6 cut(s) 430, 537, 643, 883, 1701, 1705
BtsI GCAGTG 2 cut(s) 329, 1567
BtsIMutI CAGTG 7 cut(s) 329, 711, 785, 975, 1446, 1567, 2214
Cac8I GCNNGC 6 cut(s) 323, 466, 470, 946, 2255, 2407
CaiI CAGNNNCTG 1 cut(s) 1970
CfoI GCGC 3 cut(s) 243, 502, 983
Cfr10I RCCGGY 1 cut(s) 445
Cfr13I GGNCC 4 cut(s) 192, 193, 208, 370
CseI GACGC 5 cut(s) 83, 247, 2285, 2641, 2665
Csp6I GTAC 5 cut(s) 16, 48, 725, 783, 2597
CviAII CATG 9 cut(s) 1060, 1132, 1174, 1246, 1379, 1480, 1553, 2641, 2688
CviQI GTAC 5 cut(s) 16, 48, 725, 783, 2597
DraI TTTAAA 1 cut(s) 2341
DrdI GACNNNNNNGTC 3 cut(s) 95, 215, 260
DriI GACNNNNNGTC 1 cut(s) 1348
DseDI GACNNNNNNGTC 3 cut(s) 95, 215, 260
EaeI YGGCCR 2 cut(s) 19, 2281
EagI CGGCCG 1 cut(s) 19
Eam1104I CTCTTC 2 cut(s) 1385, 1650
Eam1105I GACNNNNNGTC 1 cut(s) 1348
EarI CTCTTC 2 cut(s) 1385, 1650
EclXI CGGCCG 1 cut(s) 19
Eco130I CCWWGG 5 cut(s) 222, 1059, 1245, 1479, 2398
Eco147I AGGCCT 1 cut(s) 2253
Eco24I GRGCYC 2 cut(s) 196, 432
Eco31I GGTCTC 2 cut(s) 569, 1610
Eco32I GATATC 1 cut(s) 2646
Eco47I GGWCC 1 cut(s) 208
Eco47III AGCGCT 1 cut(s) 242
Eco52I CGGCCG 1 cut(s) 19
Eco57I CTGAAG 2 cut(s) 735, 2137
Eco72I CACGTG 1 cut(s) 400
Eco88I CYCGRG 1 cut(s) 2651
Eco91I GGTNACC 1 cut(s) 1054
EcoO65I GGTNACC 1 cut(s) 1054
EcoRII CCWGG 4 cut(s) 372, 1143, 1691, 2583
EcoRV GATATC 1 cut(s) 2646
EcoT14I CCWWGG 5 cut(s) 222, 1059, 1245, 1479, 2398
EcoT38I GRGCYC 2 cut(s) 196, 432
ErhI CCWWGG 5 cut(s) 222, 1059, 1245, 1479, 2398
Esp3I CGTCTC 4 cut(s) 171, 2151, 2216, 2648
FaeI CATG 9 cut(s) 1063, 1135, 1177, 1249, 1382, 1483, 1556, 2644, 2691
FalI AAGNNNNNCTT 2 cut(s) 825, 857
FaqI GGGAC 2 cut(s) 508, 1826
FatI CATG 9 cut(s) 1059, 1131, 1173, 1245, 1378, 1479, 1552, 2640, 2687
FauI CCCGC 1 cut(s) 259
FbaI TGATCA 2 cut(s) 640, 2218
FblI GTMKAC 1 cut(s) 1445
FokI GGATG 6 cut(s) 437, 544, 650, 890, 1708, 1712
FriOI GRGCYC 2 cut(s) 196, 432
FspBI CTAG 9 cut(s) 465, 749, 1220, 1521, 2310, 2325, 2390, 2516, 2594
GlaI GCGC 3 cut(s) 242, 501, 982
GsaI CCCAGC 1 cut(s) 588
GsuI CTGGAG 1 cut(s) 787
HaeII RGCGCY 1 cut(s) 244
HaeIII GGCC 6 cut(s) 21, 194, 325, 371, 2253, 2283
HapII CCGG 4 cut(s) 186, 433, 446, 899
HgaI GACGC 5 cut(s) 83, 247, 2285, 2641, 2665
HhaI GCGC 3 cut(s) 243, 502, 983
Hin1I GRCGYC 2 cut(s) 75, 258
Hin1II CATG 9 cut(s) 1063, 1135, 1177, 1249, 1382, 1483, 1556, 2644, 2691
Hin6I GCGC 3 cut(s) 241, 500, 981
HinP1I GCGC 3 cut(s) 241, 500, 981
HincII GTYRAC 1 cut(s) 1428
HindII GTYRAC 1 cut(s) 1428
HindIII AAGCTT 3 cut(s) 743, 942, 2496
HpaII CCGG 4 cut(s) 186, 433, 446, 899
HphI GGTGA 4 cut(s) 211, 573, 648, 1066
Hpy166II GTNNAC 7 cut(s) 82, 403, 783, 1428, 1446, 2263, 2317
Hpy8I GTNNAC 7 cut(s) 82, 403, 783, 1428, 1446, 2263, 2317
Hpy99I CGWCG 7 cut(s) 27, 77, 89, 92, 95, 263, 266
HpyAV CCTTC 9 cut(s) 121, 289, 430, 453, 1812, 1976, 2018, 2161, 2327
HpyCH4III ACNGT 3 cut(s) 665, 2567, 2700
HpyCH4IV ACGT 6 cut(s) 14, 216, 399, 739, 2209, 2548
HpyF10VI GCNNNNNNNGC 9 cut(s) 247, 331, 334, 478, 508, 511, 1557, 2504, 2656
HpySE526I ACGT 6 cut(s) 14, 216, 399, 739, 2209, 2548
Hsp92I GRCGYC 2 cut(s) 75, 258
Hsp92II CATG 9 cut(s) 1063, 1135, 1177, 1249, 1382, 1483, 1556, 2644, 2691
HspAI GCGC 3 cut(s) 241, 500, 981
Kpn2I TCCGGA 1 cut(s) 432
Ksp22I TGATCA 2 cut(s) 640, 2218
LmnI GCTCC 3 cut(s) 394, 435, 1140
LweI GCATC 5 cut(s) 1030, 1477, 1690, 1928, 2542
MaeI CTAG 9 cut(s) 465, 749, 1220, 1521, 2310, 2325, 2390, 2516, 2594
MaeII ACGT 6 cut(s) 14, 216, 399, 739, 2209, 2548
MaeIII GTNAC 2 cut(s) 217, 1054
MfeI CAATTG 2 cut(s) 696, 1892
MflI RGATCY 6 cut(s) 1240, 1394, 1666, 1718, 1768, 2359
MhlI GDGCHC 3 cut(s) 196, 399, 432
MlsI TGGCCA 1 cut(s) 2283
MluNI TGGCCA 1 cut(s) 2283
MmeI TCCRAC 3 cut(s) 81, 1218, 2586
Mox20I TGGCCA 1 cut(s) 2283
MroI TCCGGA 1 cut(s) 432
MroXI GAANNNNTTC 2 cut(s) 1528, 1835
MscI TGGCCA 1 cut(s) 2283
MseI TTAA 3 cut(s) 669, 1008, 2340
MslI CAYNNNNRTG 2 cut(s) 1478, 2199
Msp20I TGGCCA 1 cut(s) 2283
MspA1I CMGCKG 2 cut(s) 250, 2453
MspI CCGG 4 cut(s) 186, 433, 446, 899
MspR9I CCNGG 5 cut(s) 187, 374, 1145, 1693, 2585
MunI CAATTG 2 cut(s) 696, 1892
Mva1269I GAATGC 4 cut(s) 39, 1603, 1861, 2621
MvaI CCWGG 4 cut(s) 374, 1145, 1693, 2585
MvnI CGCG 2 cut(s) 266, 2296
MwoI GCNNNNNNNGC 9 cut(s) 247, 331, 334, 478, 508, 511, 1557, 2504, 2656
NciI CCSGG 1 cut(s) 187
NcoI CCATGG 3 cut(s) 1059, 1245, 1479
NheI GCTAGC 1 cut(s) 464
NlaIII CATG 9 cut(s) 1063, 1135, 1177, 1249, 1382, 1483, 1556, 2644, 2691
NlaIV GGNNCC 6 cut(s) 194, 210, 431, 1142, 1242, 1987
NmeAIII GCCGAG 1 cut(s) 891
NmuCI GTSAC 2 cut(s) 217, 1054
NspI RCATGY 1 cut(s) 1382
PceI AGGCCT 1 cut(s) 2253
PciI ACATGT 1 cut(s) 1378
PcsI WCGNNNNNNNCGW 3 cut(s) 81, 90, 1680
PctI GAATGC 4 cut(s) 39, 1603, 1861, 2621
PdmI GAANNNNTTC 2 cut(s) 1528, 1835
PfeI GAWTC 7 cut(s) 203, 578, 653, 1204, 1566, 1688, 2684
Pfl23II CGTACG 1 cut(s) 15
PflFI GACNNNGTC 3 cut(s) 95, 257, 407
PflMI CCANNNNNTGG 1 cut(s) 734
PfoI TCCNGGA 1 cut(s) 1691
PmaCI CACGTG 1 cut(s) 400
PmlI CACGTG 1 cut(s) 400
Ppu21I YACGTR 2 cut(s) 400, 2549
PscI ACATGT 1 cut(s) 1378
PshAI GACNNNNGTC 1 cut(s) 1887
PshBI ATTAAT 1 cut(s) 669
PsiI TTATAA 2 cut(s) 1280, 1797
Psp6I CCWGG 4 cut(s) 372, 1143, 1691, 2583
PspCI CACGTG 1 cut(s) 400
PspEI GGTNACC 1 cut(s) 1054
PspFI CCCAGC 1 cut(s) 584
PspGI CCWGG 4 cut(s) 372, 1143, 1691, 2583
PspLI CGTACG 1 cut(s) 15
PspN4I GGNNCC 6 cut(s) 194, 210, 431, 1142, 1242, 1987
PspOMI GGGCCC 1 cut(s) 192
PspPI GGNCC 4 cut(s) 192, 193, 208, 370
PstI CTGCAG 1 cut(s) 323
PstNI CAGNNNCTG 1 cut(s) 1970
PsuI RGATCY 6 cut(s) 1240, 1394, 1666, 1718, 1768, 2359
PsyI GACNNNGTC 3 cut(s) 95, 257, 407
PvuII CAGCTG 1 cut(s) 2453
RsaI GTAC 5 cut(s) 17, 49, 726, 784, 2598
RsaNI GTAC 5 cut(s) 16, 48, 725, 783, 2597
RseI CAYNNNNRTG 2 cut(s) 1478, 2199
SaqAI TTAA 3 cut(s) 669, 1008, 2340
Sau96I GGNCC 4 cut(s) 192, 193, 208, 370
SbfI CCTGCAGG 1 cut(s) 323
ScaI AGTACT 1 cut(s) 49
ScrFI CCNGG 5 cut(s) 187, 374, 1145, 1693, 2585
SdaI CCTGCAGG 1 cut(s) 323
SduI GDGCHC 3 cut(s) 196, 399, 432
SfaNI GCATC 5 cut(s) 1030, 1477, 1690, 1928, 2542
SfcI CTRYAG 2 cut(s) 319, 1313
SinI GGWCC 1 cut(s) 208
SmiMI CAYNNNNRTG 2 cut(s) 1478, 2199
Sse8387I CCTGCAGG 1 cut(s) 323
SseBI AGGCCT 1 cut(s) 2253
SspI AATATT 1 cut(s) 1294
SspMI CTAG 9 cut(s) 465, 749, 1220, 1521, 2310, 2325, 2390, 2516, 2594
StuI AGGCCT 1 cut(s) 2253
StyD4I CCNGG 5 cut(s) 185, 372, 1143, 1691, 2583
StyI CCWWGG 5 cut(s) 222, 1059, 1245, 1479, 2398
TaaI ACNGT 3 cut(s) 665, 2567, 2700
TaiI ACGT 6 cut(s) 17, 219, 402, 742, 2212, 2551
TaqI TCGA 3 cut(s) 201, 356, 1128
TatI WGTACW 1 cut(s) 47
TauI GCSGC 5 cut(s) 136, 328, 337, 514, 815
TfiI GAWTC 7 cut(s) 203, 578, 653, 1204, 1566, 1688, 2684
Tru1I TTAA 3 cut(s) 669, 1008, 2340
Tru9I TTAA 3 cut(s) 669, 1008, 2340
TscAI CASTG 7 cut(s) 336, 718, 785, 982, 1446, 1567, 2221
TseFI GTSAC 2 cut(s) 217, 1054
Tsp45I GTSAC 2 cut(s) 217, 1054
TspDTI ATGAA 4 cut(s) 1241, 1485, 1800, 2048
TspRI CASTG 7 cut(s) 336, 718, 785, 982, 1446, 1567, 2221
Tth111I GACNNNGTC 3 cut(s) 95, 257, 407
Van91I CCANNNNNTGG 1 cut(s) 734
VpaK11BI GGWCC 1 cut(s) 208
VspI ATTAAT 1 cut(s) 669
XapI RAATTY 2 cut(s) 837, 1574
XbaI TCTAGA 1 cut(s) 1520
XceI RCATGY 1 cut(s) 1382
XcmI CCANNNNNNNNNTGG 2 cut(s) 1477, 1559
XmiI GTMKAC 1 cut(s) 1445
XmnI GAANNNNTTC 2 cut(s) 1528, 1835
XspI CTAG 9 cut(s) 465, 749, 1220, 1521, 2310, 2325, 2390, 2516, 2594
ZrmI AGTACT 1 cut(s) 49
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.