AT1G61720

NmrA-like family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0010753
Physical Location & Seq
Reverse (-)
22790966 .. 22792803
1838 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G61720.1

Sequence Viewer

Length: 1023 bp
ATGGACCAGACTCTTACACACACCGGATCGAAGAAGGCTTGTGTCATTGGTGGCACGGGAAACTTAGCCTCTATTCTCATCAAGCATTTGCTTCAAAGTGGCTACAAAGTTAACACTACAGTTAGAGATCCAGAAAACGAGAAGAAAATAGCTCACCTTAGGAAACTTCAAGAGCTTGGCGACCTGAAGATCTTCAAGGCAGATTTGACTGATGAAGACAGTTTCGAATCCTCATTCTCCGGCTGTGAATACATCTTCCATGTCGCAACTCCGATCAACTTTAAATCCGAAGATCCCGAGAAAGACATGATCAAGCCGGCGATACAAGGAGTGATCAATGTGTTGAAATCTTGCTTAAAATCGAAATCAGTCAAGCGTGTGATCTACACATCTTCAGCTGCTGCTGTTTCCATCAACAATCTTTCTGGAACCGGAATCGTGATGAACGAAGAAAACTGGACTGACGTTGAATTTCTCACAGAGGAGAAGCCTTTTAACTGGGGTTACCCAATCTCGAAGGTGTTAGCAGAAAAGACAGCTTGGGAATTTGCAAAAGAGAATAAGATCAATCTCGTTACCGTGATTCCGGCACTTATAGCCGGAAACTCTCTCCTCTCCGATCCTCCGAGTAGCTTATCTCTCTCGATGTCTTTCATCACCGGGAAAGAAATGCATGTGACCGGTCTCAAGGAAATGCAGAAGCTATCTGGCTCGATCTCGTTCGTCCACGTAGACGATTTAGCTCGTGCCCATTTGTTTCTTGCGGAGAAAGAAACTGCTTCTGGTCGCTACATTTGCTGTGCTTACAACACAAGTGTTCCAGAGATTGCGGATTTTCTCATACAGAGATATCCTAAGTACAATGTGTTGTCAGAATTCGAAGAGGGCTTGTCGATTCCGAAATTGACACTATCTTCGCAAAAACTTATCAATGAAGGCTTTCGATTCGAATATGGGATCAATGAGATGTATGATCAAATGATAGAGTACTTCGAGTCAAAAGGATTGATCAAAGCTAAATAA

Protein Analysis

340

Amino Acids

37.91

Weight (kDa)

5.89

Isoelectric Point (pI)

26.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 11 - 141 2.8e-11 NmrA-like family
Epimerase PF01370 14 - 259 1.2e-26 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 15 - 182 6.7e-18 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 15 - 134 3e-09 GDP-mannose 4,6 dehydratase
KR PF08659 15 - 137 6.1e-06 KR domain
NAD_binding_4 PF07993 17 - 248 1.9e-15 Male sterility protein
NAD_binding_10 PF13460 17 - 135 5.3e-10 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 732
AciI CCGC 2 cut(s) 764, 830
AclWI GGATC 5 cut(s) 34, 122, 287, 614, 965
AcsI RAATTY 3 cut(s) 470, 545, 875
AcuI CTGAAG 2 cut(s) 206, 378
AfaI GTAC 2 cut(s) 860, 989
AgeI ACCGGT 1 cut(s) 680
AgsI TTSAA 5 cut(s) 95, 170, 196, 346, 470
AluBI AGCT 8 cut(s) 152, 175, 398, 539, 633, 703, 743, 1016
AluI AGCT 8 cut(s) 152, 175, 398, 539, 633, 703, 743, 1016
Alw26I GTCTC 1 cut(s) 689
AlwI GGATC 5 cut(s) 34, 122, 287, 614, 965
AlwNI CAGNNNCTG 1 cut(s) 401
Ama87I CYCGRG 1 cut(s) 296
ApeKI GCWGC 2 cut(s) 398, 401
ApoI RAATTY 3 cut(s) 470, 545, 875
ArsI GACNNNNNNTTYG 2 cut(s) 898, 930
AsiGI ACCGGT 1 cut(s) 680
Asp700I GAANNNNTTC 2 cut(s) 191, 939
AspS9I GGNCC 1 cut(s) 4
AsuC2I CCSGG 1 cut(s) 661
AsuHPI GGTGA 2 cut(s) 146, 649
AsuII TTCGAA 3 cut(s) 225, 879, 948
AvaI CYCGRG 1 cut(s) 296
AvaII GGWCC 1 cut(s) 4
AxyI CCTNAGG 1 cut(s) 158
BaeGI GKGCMC 1 cut(s) 751
BauI CACGAG 1 cut(s) 744
BbsI GAAGAC 1 cut(s) 222
BbvI GCAGC 2 cut(s) 385, 388
BccI CCATC 1 cut(s) 419
BclI TGATCA 4 cut(s) 309, 333, 973, 1008
BcnI CCSGG 1 cut(s) 661
BcoDI GTCTC 1 cut(s) 689
BfmI CTRYAG 1 cut(s) 117
BglII AGATCT 1 cut(s) 189
BisI GCNGC 2 cut(s) 399, 402
BlsI GCNGC 2 cut(s) 400, 403
BmcAI AGTACT 1 cut(s) 989
Bme1390I CCNGG 1 cut(s) 661
Bme18I GGWCC 1 cut(s) 4
BmeT110I CYCGRG 1 cut(s) 296
BmgT120I GGNCC 1 cut(s) 4
BmiI GGNNCC 1 cut(s) 430
BmrFI CCNGG 1 cut(s) 661
BmrI ACTGGG 1 cut(s) 508
BmuI ACTGGG 1 cut(s) 508
BpiI GAAGAC 1 cut(s) 222
Bpu14I TTCGAA 3 cut(s) 225, 879, 948
BpuEI CTTGAG 1 cut(s) 671
BpuMI CCSGG 1 cut(s) 661
BsaAI YACGTR 1 cut(s) 730
BsaI GGTCTC 1 cut(s) 689
BsaWI WCCGGW 3 cut(s) 23, 431, 680
Bse118I RCCGGY 2 cut(s) 316, 680
Bse1I ACTGG 2 cut(s) 461, 503
Bse21I CCTNAGG 1 cut(s) 158
BseNI ACTGG 2 cut(s) 461, 503
BseRI GAGGAG 2 cut(s) 497, 602
BseSI GKGCMC 1 cut(s) 751
BseXI GCAGC 2 cut(s) 385, 388
BshTI ACCGGT 1 cut(s) 680
BsiHKCI CYCGRG 1 cut(s) 296
BsiSI CCGG 8 cut(s) 24, 240, 317, 432, 587, 600, 660, 681
BsmAI GTCTC 1 cut(s) 689
Bso31I GGTCTC 1 cut(s) 689
BsoBI CYCGRG 1 cut(s) 296
Bsp119I TTCGAA 3 cut(s) 225, 879, 948
Bsp1286I GDGCHC 1 cut(s) 751
BspACI CCGC 2 cut(s) 764, 830
BspLI GGNNCC 1 cut(s) 430
BspPI GGATC 5 cut(s) 34, 122, 287, 614, 965
BspT104I TTCGAA 3 cut(s) 225, 879, 948
BspTNI GGTCTC 1 cut(s) 689
BsrFI RCCGGY 2 cut(s) 316, 680
BsrI ACTGG 2 cut(s) 461, 503
BssAI RCCGGY 2 cut(s) 316, 680
BssSI CACGAG 1 cut(s) 744
Bst2BI CACGAG 1 cut(s) 744
Bst4CI ACNGT 3 cut(s) 121, 221, 580
Bst6I CTCTTC 1 cut(s) 876
BstBAI YACGTR 1 cut(s) 730
BstBI TTCGAA 3 cut(s) 225, 879, 948
BstC8I GCNNGC 1 cut(s) 318
BstDEI CTNAG 3 cut(s) 64, 158, 855
BstEII GGTNACC 1 cut(s) 503
BstMAI GTCTC 1 cut(s) 689
BstMWI GCNNNNNNNGC 2 cut(s) 596, 795
BstNSI RCATGY 1 cut(s) 677
BstPI GGTNACC 1 cut(s) 503
BstSCI CCNGG 1 cut(s) 659
BstSFI CTRYAG 1 cut(s) 117
BstSLI GKGCMC 1 cut(s) 751
BstV1I GCAGC 2 cut(s) 385, 388
BstV2I GAAGAC 1 cut(s) 222
BstX2I RGATCY 3 cut(s) 127, 189, 292
BstYI RGATCY 3 cut(s) 127, 189, 292
Bsu36I CCTNAGG 1 cut(s) 158
Cac8I GCNNGC 1 cut(s) 318
CaiI CAGNNNCTG 1 cut(s) 401
Cfr10I RCCGGY 2 cut(s) 316, 680
Cfr13I GGNCC 1 cut(s) 4
Csp6I GTAC 2 cut(s) 859, 988
CspAI ACCGGT 1 cut(s) 680
CviAII CATG 3 cut(s) 260, 307, 674
CviQI GTAC 2 cut(s) 859, 988
DdeI CTNAG 3 cut(s) 64, 158, 855
DraI TTTAAA 1 cut(s) 283
Eam1104I CTCTTC 1 cut(s) 876
EarI CTCTTC 1 cut(s) 876
Eco31I GGTCTC 1 cut(s) 689
Eco32I GATATC 1 cut(s) 851
Eco47I GGWCC 1 cut(s) 4
Eco57I CTGAAG 2 cut(s) 206, 378
Eco81I CCTNAGG 1 cut(s) 158
Eco88I CYCGRG 1 cut(s) 296
Eco91I GGTNACC 1 cut(s) 503
EcoO65I GGTNACC 1 cut(s) 503
EcoRI GAATTC 1 cut(s) 875
EcoRV GATATC 1 cut(s) 851
EcoT22I ATGCAT 1 cut(s) 675
FaeI CATG 3 cut(s) 263, 310, 677
FaiI YATR 7 cut(s) 261, 308, 596, 675, 842, 954, 972
FatI CATG 3 cut(s) 259, 306, 673
FbaI TGATCA 4 cut(s) 309, 333, 973, 1008
FblI GTMKAC 1 cut(s) 732
Fnu4HI GCNGC 2 cut(s) 399, 402
Fsp4HI GCNGC 2 cut(s) 399, 402
GluI GCNGC 2 cut(s) 399, 402
HapII CCGG 8 cut(s) 24, 240, 317, 432, 587, 600, 660, 681
Hin1II CATG 3 cut(s) 263, 310, 677
HincII GTYRAC 1 cut(s) 112
HindII GTYRAC 1 cut(s) 112
HinfI GANTC 7 cut(s) 10, 227, 435, 583, 895, 945, 995
HpaI GTTAAC 1 cut(s) 112
HpaII CCGG 8 cut(s) 24, 240, 317, 432, 587, 600, 660, 681
HphI GGTGA 2 cut(s) 146, 649
Hpy166II GTNNAC 3 cut(s) 112, 727, 733
Hpy188I TCNGA 6 cut(s) 273, 289, 619, 627, 874, 900
Hpy188III TCNNGA 8 cut(s) 131, 170, 296, 426, 439, 514, 643, 821
Hpy8I GTNNAC 3 cut(s) 112, 727, 733
HpyAV CCTTC 3 cut(s) 28, 511, 929
HpyCH4III ACNGT 3 cut(s) 121, 221, 580
HpyCH4IV ACGT 2 cut(s) 465, 729
HpyCH4V TGCA 3 cut(s) 551, 673, 697
HpyF10VI GCNNNNNNNGC 2 cut(s) 596, 795
HpyF3I CTNAG 3 cut(s) 64, 158, 855
HpySE526I ACGT 2 cut(s) 465, 729
Hsp92II CATG 3 cut(s) 263, 310, 677
KroI GCCGGC 1 cut(s) 316
KroNI GCCGGC 1 cut(s) 318
Ksp22I TGATCA 4 cut(s) 309, 333, 973, 1008
KspAI GTTAAC 1 cut(s) 112
Lsp1109I GCAGC 2 cut(s) 385, 388
MaeII ACGT 2 cut(s) 465, 729
MaeIII GTNAC 3 cut(s) 503, 574, 676
MflI RGATCY 3 cut(s) 127, 189, 292
MhlI GDGCHC 1 cut(s) 751
MluCI AATT 4 cut(s) 470, 545, 875, 902
MlyI GAGTC 2 cut(s) 4, 1004
MnlI CCTC 6 cut(s) 79, 241, 475, 623, 633, 877
Mph1103I ATGCAT 1 cut(s) 675
MroNI GCCGGC 1 cut(s) 316
MroXI GAANNNNTTC 2 cut(s) 191, 939
MseI TTAA 4 cut(s) 111, 282, 356, 495
MspA1I CMGCKG 1 cut(s) 398
MspI CCGG 8 cut(s) 24, 240, 317, 432, 587, 600, 660, 681
MspR9I CCNGG 1 cut(s) 661
MwoI GCNNNNNNNGC 2 cut(s) 596, 795
NaeI GCCGGC 1 cut(s) 318
NciI CCSGG 1 cut(s) 661
NgoMIV GCCGGC 1 cut(s) 316
NlaIII CATG 3 cut(s) 263, 310, 677
NlaIV GGNNCC 1 cut(s) 430
NmuCI GTSAC 1 cut(s) 676
NsiI ATGCAT 1 cut(s) 675
NspI RCATGY 1 cut(s) 677
NspV TTCGAA 3 cut(s) 225, 879, 948
PcsI WCGNNNNNNNCGW 1 cut(s) 444
PdiI GCCGGC 1 cut(s) 318
PdmI GAANNNNTTC 2 cut(s) 191, 939
PfeI GAWTC 5 cut(s) 227, 435, 583, 895, 945
PinAI ACCGGT 1 cut(s) 680
PkrI GCNGC 2 cut(s) 400, 403
PleI GAGTC 2 cut(s) 4, 1003
PpsI GAGTC 2 cut(s) 4, 1003
Ppu21I YACGTR 1 cut(s) 730
PspEI GGTNACC 1 cut(s) 503
PspN4I GGNNCC 1 cut(s) 430
PspPI GGNCC 1 cut(s) 4
PstNI CAGNNNCTG 1 cut(s) 401
PsuI RGATCY 3 cut(s) 127, 189, 292
PvuII CAGCTG 1 cut(s) 398
RsaI GTAC 2 cut(s) 860, 989
RsaNI GTAC 2 cut(s) 859, 988
SaqAI TTAA 4 cut(s) 111, 282, 356, 495
SatI GCNGC 2 cut(s) 399, 402
Sau96I GGNCC 1 cut(s) 4
ScaI AGTACT 1 cut(s) 989
SchI GAGTC 2 cut(s) 4, 1004
ScrFI CCNGG 1 cut(s) 661
SduI GDGCHC 1 cut(s) 751
SfcI CTRYAG 1 cut(s) 117
SfuI TTCGAA 3 cut(s) 225, 879, 948
SinI GGWCC 1 cut(s) 4
SmlI CTYRAG 1 cut(s) 686
SmoI CTYRAG 1 cut(s) 686
Sse9I AATT 4 cut(s) 470, 545, 875, 902
SsiI CCGC 2 cut(s) 764, 830
StyD4I CCNGG 1 cut(s) 659
TaaI ACNGT 3 cut(s) 121, 221, 580
TaiI ACGT 2 cut(s) 468, 732
TasI AATT 4 cut(s) 470, 545, 875, 902
TatI WGTACW 2 cut(s) 858, 987
TfiI GAWTC 5 cut(s) 227, 435, 583, 895, 945
Tru1I TTAA 4 cut(s) 111, 282, 356, 495
Tru9I TTAA 4 cut(s) 111, 282, 356, 495
TseFI GTSAC 1 cut(s) 676
TseI GCWGC 2 cut(s) 398, 401
Tsp45I GTSAC 1 cut(s) 676
TspDTI ATGAA 4 cut(s) 228, 458, 643, 948
VpaK11BI GGWCC 1 cut(s) 4
XapI RAATTY 3 cut(s) 470, 545, 875
XceI RCATGY 1 cut(s) 677
XmiI GTMKAC 1 cut(s) 732
XmnI GAANNNNTTC 2 cut(s) 191, 939
ZrmI AGTACT 1 cut(s) 989
Zsp2I ATGCAT 1 cut(s) 675
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.