FvH4_3g02980

Anthocyanidin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0007662
Physical Location & Seq
Forward (+)
1583755 .. 1586819
3065 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g02980.t2

Sequence Viewer

Length: 1026 bp
ATGTCCACCACCCAACCCATCATCTCAACAAAGTCTGCTTGTGTCATCGGCGGCACCGGCTTCGTGGCGTCTCAGCTGATCAAGCTCTTACTAGAGAAGGGCTATGCCGTCAGAACCACTGTTAGAGACCCAGATAATCTGAAGAAGATCTCCCACCTAACAGCACTACAAGAGTTGGGAGAGCTAACAATATTTCGTGGGGATTTAACCGATGAAGGGAGCTTTGATGCTGCTATAGCAGGTTCTGATCTTGTTTTCCATGTAGCCACACCAGTCCACTTTGGCTCGCCGGATCCAGAGAACGACATGATCAAGCCAGGAGTCCAAGGAGTACTAAACGTTATGAAATCATGTGTGAAAGCAAAAACAGTTAAACGAGTCGTTTTGACATCATCAGCAGCTGCAGTAACTGTCAATACTCTTAGTGGAACAGGCCTGATCGCCGACGAAAATGATTGGTCTGATGTTGAGTTCTTGACCACTGCCAAGCCACCTACCTGGGGATATCCTGTTTCAAAGGTACTAGCTGAGAAGACAGCATGGAAATTTGCTGAACAAAACAACATTGATCTCATCACTGTGATCCCTTCTCTCATGGCTGGTGCTTCTCTCACTCCAGACATCCCCAGCAGTATAGGCCTCGCCACGTCTTTAATTACAGGAAATGAGTTCCTCATAAAAGGCTTGAAAGGCATGCAAATGCTATCAGGTTCCATATCCATTACACATGTGGAGGATGTCTGCCGAGCTCATATATTTTTGGCAGAGAAAGAATCTGCTTCTGGTCGGTACATATGCTGTGCTGAAAATAGTAGTGTTCCTGAGGTTGCAAAGTTCCTCAGCAAAAGATATCCCGAATACAAAGTCCCGACTGAGTTTGGAGATTTTCCATCCAAGGCGAAGACCATACTCTCTTCAGAAAAGCTTAAGAAGGAGGGGTTCACTTTCAAGTACGGGATTGAAGACATATATGACCAAACTGTGGAGTACTTGAAACTTAAGGGGGTGCTGCAGAACAAGAACTAG

Protein Analysis

342

Amino Acids

36.86

Weight (kDa)

6.25

Isoelectric Point (pI)

28.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Epimerase PF01370 27 - 243 8e-18 NAD dependent epimerase/dehydratase family
NAD_binding_10 PF13460 34 - 119 1.7e-06 NAD(P)H-binding
3Beta_HSD PF01073 35 - 168 2.7e-12 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4 PF07993 56 - 195 5.1e-10 Male sterility protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 230
AccB1I GGYRCC 1 cut(s) 53
AccB7I CCANNNNNTGG 1 cut(s) 982
AciI CCGC 1 cut(s) 51
AclI AACGTT 1 cut(s) 339
AclWI GGATC 3 cut(s) 287, 300, 577
AcsI RAATTY 1 cut(s) 545
AcuI CTGAAG 2 cut(s) 161, 900
AcyI GRCGYC 1 cut(s) 68
AfaI GTAC 5 cut(s) 333, 522, 791, 953, 989
AfiI CCNNNNNNNGG 3 cut(s) 216, 500, 982
AflII CTTAAG 2 cut(s) 926, 998
AflIII ACRYGT 1 cut(s) 727
AgsI TTSAA 5 cut(s) 516, 688, 949, 962, 994
AjiI CACGTC 1 cut(s) 648
AjnI CCWGG 2 cut(s) 316, 497
AluBI AGCT 8 cut(s) 76, 85, 184, 222, 401, 527, 749, 925
AluI AGCT 8 cut(s) 76, 85, 184, 222, 401, 527, 749, 925
Alw21I GWGCWC 1 cut(s) 751
Alw26I GTCTC 2 cut(s) 75, 120
AlwI GGATC 3 cut(s) 287, 300, 577
AlwNI CAGNNNCTG 3 cut(s) 245, 401, 410
AoxI GGCC 2 cut(s) 433, 637
ApeKI GCWGC 4 cut(s) 230, 398, 401, 1009
ApoI RAATTY 1 cut(s) 545
ArsI GACNNNNNNTTYG 2 cut(s) 849, 881
AxyI CCTNAGG 1 cut(s) 822
BamHI GGATCC 1 cut(s) 292
BanI GGYRCC 1 cut(s) 53
BanII GRGCYC 1 cut(s) 751
BbsI GAAGAC 3 cut(s) 539, 908, 969
Bbv12I GWGCWC 1 cut(s) 751
BbvCI CCTCAGC 1 cut(s) 839
BbvI GCAGC 4 cut(s) 217, 388, 410, 996
BccI CCATC 2 cut(s) 26, 898
BceAI ACGGC 1 cut(s) 92
BcgI CGANNNNNNTGC 2 cut(s) 43, 77
BciT130I CCWGG 2 cut(s) 318, 499
BclI TGATCA 2 cut(s) 78, 309
BcoDI GTCTC 2 cut(s) 75, 120
BfaI CTAG 3 cut(s) 92, 524, 1024
BfmI CTRYAG 3 cut(s) 234, 402, 1010
BfrI CTTAAG 2 cut(s) 926, 998
BfuAI ACCTGC 1 cut(s) 230
BglII AGATCT 1 cut(s) 147
BisI GCNGC 5 cut(s) 52, 231, 399, 402, 1010
BlsI GCNGC 5 cut(s) 53, 232, 400, 403, 1011
BmcAI AGTACT 2 cut(s) 333, 989
Bme1390I CCNGG 2 cut(s) 318, 499
BmgBI CACGTC 1 cut(s) 648
BmiI GGNNCC 3 cut(s) 55, 294, 712
BmrFI CCNGG 2 cut(s) 318, 499
BmsI GCATC 1 cut(s) 217
BpiI GAAGAC 3 cut(s) 539, 908, 969
BpmI CTGGAG 1 cut(s) 600
Bpu10I CCTNAGC 1 cut(s) 839
BsaHI GRCGYC 1 cut(s) 68
BsaI GGTCTC 1 cut(s) 120
BsaJI CCNNGG 3 cut(s) 325, 498, 894
Bsc4I CCNNNNNNNGG 3 cut(s) 216, 500, 982
Bse118I RCCGGY 1 cut(s) 56
Bse1I ACTGG 1 cut(s) 272
Bse21I CCTNAGG 1 cut(s) 822
BseBI CCWGG 2 cut(s) 318, 499
BseDI CCNNGG 3 cut(s) 325, 498, 894
BseGI GGATG 3 cut(s) 621, 742, 890
BseLI CCNNNNNNNGG 3 cut(s) 216, 500, 982
BseMII CTCAG 5 cut(s) 86, 519, 813, 853, 864
BseNI ACTGG 1 cut(s) 272
BseXI GCAGC 4 cut(s) 217, 388, 410, 996
BseYI CCCAGC 1 cut(s) 626
BshFI GGCC 2 cut(s) 435, 639
BshNI GGYRCC 1 cut(s) 53
BsiHKAI GWGCWC 1 cut(s) 751
BsiSI CCGG 2 cut(s) 57, 290
BslFI GGGAC 1 cut(s) 851
BslI CCNNNNNNNGG 3 cut(s) 216, 500, 982
BsmAI GTCTC 2 cut(s) 75, 120
BsmBI CGTCTC 1 cut(s) 75
BsmFI GGGAC 1 cut(s) 851
BsnI GGCC 2 cut(s) 435, 639
Bso31I GGTCTC 1 cut(s) 120
Bsp1286I GDGCHC 1 cut(s) 751
Bsp143I GATC 8 cut(s) 78, 147, 247, 292, 309, 438, 568, 582
BspACI CCGC 1 cut(s) 51
BspANI GGCC 2 cut(s) 435, 639
BspCNI CTCAG 5 cut(s) 85, 520, 814, 852, 865
BspLI GGNNCC 3 cut(s) 55, 294, 712
BspMAI CTGCAG 2 cut(s) 406, 1014
BspMI ACCTGC 1 cut(s) 230
BspPI GGATC 3 cut(s) 287, 300, 577
BspT107I GGYRCC 1 cut(s) 53
BspTI CTTAAG 2 cut(s) 926, 998
BspTNI GGTCTC 1 cut(s) 120
BsrFI RCCGGY 1 cut(s) 56
BsrI ACTGG 1 cut(s) 272
BssAI RCCGGY 1 cut(s) 56
BssECI CCNNGG 3 cut(s) 325, 498, 894
BssMI GATC 8 cut(s) 78, 147, 247, 292, 309, 438, 568, 582
BssNI GRCGYC 1 cut(s) 68
BssT1I CCWWGG 2 cut(s) 325, 894
Bst2UI CCWGG 2 cut(s) 318, 499
Bst4CI ACNGT 5 cut(s) 121, 370, 412, 580, 982
Bst6I CTCTTC 1 cut(s) 919
BstACI GRCGYC 1 cut(s) 68
BstAFI CTTAAG 2 cut(s) 926, 998
BstC8I GCNNGC 2 cut(s) 287, 695
BstDEI CTNAG 6 cut(s) 72, 422, 528, 822, 839, 873
BstF5I GGATG 3 cut(s) 621, 742, 890
BstKTI GATC 8 cut(s) 81, 150, 250, 295, 312, 441, 571, 585
BstMAI GTCTC 2 cut(s) 75, 120
BstMBI GATC 8 cut(s) 78, 147, 247, 292, 309, 438, 568, 582
BstMWI GCNNNNNNNGC 5 cut(s) 57, 82, 236, 636, 690
BstNI CCWGG 2 cut(s) 318, 499
BstNSI RCATGY 2 cut(s) 697, 731
BstSCI CCNGG 2 cut(s) 316, 497
BstSFI CTRYAG 3 cut(s) 234, 402, 1010
BstV1I GCAGC 4 cut(s) 217, 388, 410, 996
BstV2I GAAGAC 3 cut(s) 539, 908, 969
BstX2I RGATCY 2 cut(s) 147, 292
BstXI CCANNNNNNTGG 1 cut(s) 498
BstYI RGATCY 2 cut(s) 147, 292
Bsu36I CCTNAGG 1 cut(s) 822
BsuRI GGCC 2 cut(s) 435, 639
BtrI CACGTC 1 cut(s) 648
BtsCI GGATG 3 cut(s) 621, 742, 890
BtsI GCAGTG 1 cut(s) 480
BtsIMutI CAGTG 3 cut(s) 117, 480, 576
BveI ACCTGC 1 cut(s) 230
Cac8I GCNNGC 2 cut(s) 287, 695
CaiI CAGNNNCTG 3 cut(s) 245, 401, 410
Cfr10I RCCGGY 1 cut(s) 56
CseI GACGC 1 cut(s) 57
Csp6I GTAC 5 cut(s) 332, 521, 790, 952, 988
CviAII CATG 7 cut(s) 260, 307, 351, 540, 595, 694, 728
CviQI GTAC 5 cut(s) 332, 521, 790, 952, 988
DdeI CTNAG 6 cut(s) 72, 422, 528, 822, 839, 873
DpnI GATC 8 cut(s) 80, 149, 249, 294, 311, 440, 570, 584
DpnII GATC 8 cut(s) 78, 147, 247, 292, 309, 438, 568, 582
Eam1104I CTCTTC 1 cut(s) 919
EarI CTCTTC 1 cut(s) 919
Ecl136II GAGCTC 1 cut(s) 749
Eco130I CCWWGG 2 cut(s) 325, 894
Eco147I AGGCCT 2 cut(s) 435, 639
Eco24I GRGCYC 1 cut(s) 751
Eco31I GGTCTC 1 cut(s) 120
Eco32I GATATC 2 cut(s) 506, 851
Eco53kI GAGCTC 1 cut(s) 749
Eco57I CTGAAG 2 cut(s) 161, 900
Eco81I CCTNAGG 1 cut(s) 822
EcoICRI GAGCTC 1 cut(s) 749
EcoRII CCWGG 2 cut(s) 316, 497
EcoRV GATATC 2 cut(s) 506, 851
EcoT14I CCWWGG 2 cut(s) 325, 894
EcoT38I GRGCYC 1 cut(s) 751
ErhI CCWWGG 2 cut(s) 325, 894
Esp3I CGTCTC 1 cut(s) 75
FaeI CATG 7 cut(s) 263, 310, 354, 543, 598, 697, 731
FaqI GGGAC 1 cut(s) 851
FatI CATG 7 cut(s) 259, 306, 350, 539, 594, 693, 727
FauNDI CATATG 1 cut(s) 794
FbaI TGATCA 2 cut(s) 78, 309
Fnu4HI GCNGC 5 cut(s) 52, 231, 399, 402, 1010
FokI GGATG 3 cut(s) 608, 749, 877
FriOI GRGCYC 1 cut(s) 751
Fsp4HI GCNGC 5 cut(s) 52, 231, 399, 402, 1010
FspBI CTAG 3 cut(s) 92, 524, 1024
GluI GCNGC 5 cut(s) 52, 231, 399, 402, 1010
GsaI CCCAGC 1 cut(s) 630
GsuI CTGGAG 1 cut(s) 600
HaeIII GGCC 2 cut(s) 435, 639
HapII CCGG 2 cut(s) 57, 290
HgaI GACGC 1 cut(s) 57
Hin1I GRCGYC 1 cut(s) 68
Hin1II CATG 7 cut(s) 263, 310, 354, 543, 598, 697, 731
HindIII AAGCTT 1 cut(s) 923
HinfI GANTC 3 cut(s) 321, 378, 773
HpaII CCGG 2 cut(s) 57, 290
Hpy166II GTNNAC 3 cut(s) 6, 277, 942
Hpy188I TCNGA 5 cut(s) 113, 141, 247, 463, 919
Hpy188III TCNNGA 6 cut(s) 296, 475, 617, 821, 854, 868
Hpy8I GTNNAC 3 cut(s) 6, 277, 942
Hpy99I CGWCG 1 cut(s) 449
HpyAV CCTTC 4 cut(s) 91, 209, 597, 925
HpyCH4III ACNGT 5 cut(s) 121, 370, 412, 580, 982
HpyCH4IV ACGT 2 cut(s) 339, 647
HpyCH4V TGCA 4 cut(s) 404, 697, 830, 1012
HpyF10VI GCNNNNNNNGC 5 cut(s) 57, 82, 236, 636, 690
HpyF3I CTNAG 6 cut(s) 72, 422, 528, 822, 839, 873
HpySE526I ACGT 2 cut(s) 339, 647
Hsp92I GRCGYC 1 cut(s) 68
Hsp92II CATG 7 cut(s) 263, 310, 354, 543, 598, 697, 731
Ksp22I TGATCA 2 cut(s) 78, 309
Kzo9I GATC 8 cut(s) 78, 147, 247, 292, 309, 438, 568, 582
LmnI GCTCC 1 cut(s) 219
Lsp1109I GCAGC 4 cut(s) 217, 388, 410, 996
LweI GCATC 1 cut(s) 217
MaeI CTAG 3 cut(s) 92, 524, 1024
MaeII ACGT 2 cut(s) 339, 647
MaeIII GTNAC 1 cut(s) 406
MalI GATC 8 cut(s) 80, 149, 249, 294, 311, 440, 570, 584
MboI GATC 8 cut(s) 78, 147, 247, 292, 309, 438, 568, 582
MboII GAAGA 6 cut(s) 154, 157, 544, 906, 913, 974
MflI RGATCY 2 cut(s) 147, 292
MhlI GDGCHC 1 cut(s) 751
MluCI AATT 2 cut(s) 545, 654
MlyI GAGTC 2 cut(s) 330, 387
MnlI CCTC 6 cut(s) 650, 683, 727, 817, 848, 928
MseI TTAA 5 cut(s) 206, 372, 653, 927, 999
MslI CAYNNNNRTG 2 cut(s) 578, 698
MspA1I CMGCKG 2 cut(s) 76, 401
MspCI CTTAAG 2 cut(s) 926, 998
MspI CCGG 2 cut(s) 57, 290
MspR9I CCNGG 2 cut(s) 318, 499
MvaI CCWGG 2 cut(s) 318, 499
MwoI GCNNNNNNNGC 5 cut(s) 57, 82, 236, 636, 690
NdeI CATATG 1 cut(s) 794
NdeII GATC 8 cut(s) 78, 147, 247, 292, 309, 438, 568, 582
NlaIII CATG 7 cut(s) 263, 310, 354, 543, 598, 697, 731
NlaIV GGNNCC 3 cut(s) 55, 294, 712
NmeAIII GCCGAG 1 cut(s) 770
NspI RCATGY 2 cut(s) 697, 731
PaeI GCATGC 1 cut(s) 697
PceI AGGCCT 2 cut(s) 435, 639
PciI ACATGT 1 cut(s) 727
PfeI GAWTC 1 cut(s) 773
PflMI CCANNNNNTGG 1 cut(s) 982
PkrI GCNGC 5 cut(s) 53, 232, 400, 403, 1011
PleI GAGTC 2 cut(s) 329, 386
PpsI GAGTC 2 cut(s) 329, 386
PscI ACATGT 1 cut(s) 727
Psp124BI GAGCTC 1 cut(s) 751
Psp1406I AACGTT 1 cut(s) 339
Psp6I CCWGG 2 cut(s) 316, 497
PspFI CCCAGC 1 cut(s) 626
PspGI CCWGG 2 cut(s) 316, 497
PspN4I GGNNCC 3 cut(s) 55, 294, 712
PstI CTGCAG 2 cut(s) 406, 1014
PstNI CAGNNNCTG 3 cut(s) 245, 401, 410
PsuI RGATCY 2 cut(s) 147, 292
PvuII CAGCTG 2 cut(s) 76, 401
RsaI GTAC 5 cut(s) 333, 522, 791, 953, 989
RsaNI GTAC 5 cut(s) 332, 521, 790, 952, 988
RseI CAYNNNNRTG 2 cut(s) 578, 698
SacI GAGCTC 1 cut(s) 751
SaqAI TTAA 5 cut(s) 206, 372, 653, 927, 999
SatI GCNGC 5 cut(s) 52, 231, 399, 402, 1010
Sau3AI GATC 8 cut(s) 78, 147, 247, 292, 309, 438, 568, 582
ScaI AGTACT 2 cut(s) 333, 989
SchI GAGTC 2 cut(s) 330, 387
ScrFI CCNGG 2 cut(s) 318, 499
SduI GDGCHC 1 cut(s) 751
SfaNI GCATC 1 cut(s) 217
SfcI CTRYAG 3 cut(s) 234, 402, 1010
SmiMI CAYNNNNRTG 2 cut(s) 578, 698
SmlI CTYRAG 2 cut(s) 926, 998
SmoI CTYRAG 2 cut(s) 926, 998
SphI GCATGC 1 cut(s) 697
Sse9I AATT 2 cut(s) 545, 654
SseBI AGGCCT 2 cut(s) 435, 639
SsiI CCGC 1 cut(s) 51
SspI AATATT 1 cut(s) 192
SspMI CTAG 3 cut(s) 92, 524, 1024
SstI GAGCTC 1 cut(s) 751
StuI AGGCCT 2 cut(s) 435, 639
StyD4I CCNGG 2 cut(s) 316, 497
StyI CCWWGG 2 cut(s) 325, 894
TaaI ACNGT 5 cut(s) 121, 370, 412, 580, 982
TaiI ACGT 2 cut(s) 342, 650
TasI AATT 2 cut(s) 545, 654
TatI WGTACW 2 cut(s) 331, 987
TauI GCSGC 1 cut(s) 54
TfiI GAWTC 1 cut(s) 773
Tru1I TTAA 5 cut(s) 206, 372, 653, 927, 999
Tru9I TTAA 5 cut(s) 206, 372, 653, 927, 999
TscAI CASTG 3 cut(s) 124, 487, 583
TseI GCWGC 4 cut(s) 230, 398, 401, 1009
TspDTI ATGAA 2 cut(s) 228, 359
TspRI CASTG 3 cut(s) 124, 487, 583
Van91I CCANNNNNTGG 1 cut(s) 982
Vha464I CTTAAG 2 cut(s) 926, 998
XapI RAATTY 1 cut(s) 545
XceI RCATGY 2 cut(s) 697, 731
XcmI CCANNNNNNNNNTGG 1 cut(s) 727
XspI CTAG 3 cut(s) 92, 524, 1024
ZrmI AGTACT 2 cut(s) 333, 989
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.