MD10G1311200.v1.1

(2R)-phospho-3-sulfolactate synthase (ComA)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0007662
Physical Location & Seq
Reverse (-)
39624167 .. 39626570
2404 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1311200.v1.1.491

Sequence Viewer

Length: 858 bp
ATGGCGGCGTACAGATGGAAGCACTTCGAGGAGAACGAAGACCGTCCCGAAAAACCTCGAAGCTATGGCGTCACAGAAATGAGAGGCCCCCATTACTCCCTCTTGAGCCAAAACGTCCTCCAGGGTATTTTTGAGTCAATGGGGCAGTTTGTTGATGGGTTGAAGTTTTCTGGAGGTTCCGACAGCTTAATGCCGAAATCGTTCATCAAAGAAGTGACTGATGTTGCTCACCAGCATGATGTATATGTCAGCACCGGCGATTGGGCTGAAAATTTGCTTCGAAAAGGTCCGTCGGCTTTCAAAGAGTATGTGGAGGAATGTAAGAGCTTGGGGTTTGACACAATCGAGCTGAATGTGGGATCTCTTGAACTTCCTGAAGAAACTCTTCTGAGATATGTGCGCTTGATTAAGAGCGGTGGTCTGAAAGCGAAGCCACAGTTTGCGGTCAAGTTTAACAAGTCCGACATTCCTGTAGGTGATCGAGCATTTGGAGCCTACGTTGTTCCGAGCCCACGATCATCTGAATTTGTTGAAGATGTTGATCTTCTGATTAGAAGGGCTGAGAGATGCTTAGAGGCTGGGGCCGACATGATAATGATCGACGCTGATGATGTCTGTAAACAAGCTGACTCATTGCGGGCAGACATAATTGCGAAGGTTATCGGGCGCCTGGGGGTTGAGAAGACCATGTTCGAAGCTTCAAATCCAAGAACATCAGAGTGGTTCATCAAACAATATGGTCCAAGGGTGAATCTCTTCGTGGACCACTCCCATGTGATGGATCTCGAGTGCCTCCGGGGACGCAACTTAGGTAAAAATCATGCATCTGTGCTGGGTTCTTCTTATTTTCTGTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ComA

Protein Analysis

286

Amino Acids

32.1

Weight (kDa)

5.73

Isoelectric Point (pI)

36.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ComA PF02679 14 - 269 3e-74 (2R)-phospho-3-sulfolactate synthase (ComA)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 666
AccB7I CCANNNNNTGG 1 cut(s) 778
AccBSI CCGCTC 1 cut(s) 414
AciI CCGC 4 cut(s) 5, 414, 443, 637
AclWI GGATC 2 cut(s) 367, 789
AcsI RAATTY 2 cut(s) 271, 524
AcuI CTGAAG 1 cut(s) 396
AcyI GRCGYC 2 cut(s) 69, 667
AfaI GTAC 1 cut(s) 11
AfiI CCNNNNNNNGG 2 cut(s) 261, 778
AgsI TTSAA 5 cut(s) 163, 301, 368, 533, 702
AjnI CCWGG 2 cut(s) 120, 669
AluBI AGCT 6 cut(s) 63, 186, 327, 349, 626, 698
AluI AGCT 6 cut(s) 63, 186, 327, 349, 626, 698
AlwI GGATC 2 cut(s) 367, 789
Ama87I CYCGRG 1 cut(s) 785
AoxI GGCC 2 cut(s) 85, 582
ApoI RAATTY 2 cut(s) 271, 524
Asp700I GAANNNNTTC 4 cut(s) 23, 200, 384, 755
AspLEI GCGC 2 cut(s) 402, 669
AspS9I GGNCC 5 cut(s) 86, 287, 582, 740, 763
AsuC2I CCSGG 1 cut(s) 797
AsuHPI GGTGA 3 cut(s) 221, 488, 760
AsuII TTCGAA 2 cut(s) 280, 693
AvaI CYCGRG 1 cut(s) 785
AvaII GGWCC 3 cut(s) 287, 740, 763
BanI GGYRCC 1 cut(s) 666
BanII GRGCYC 1 cut(s) 512
BbsI GAAGAC 2 cut(s) 45, 689
BccI CCATC 3 cut(s) 9, 149, 772
BciT130I CCWGG 2 cut(s) 122, 671
BcnI CCSGG 1 cut(s) 797
BfmI CTRYAG 1 cut(s) 471
BfoI RGCGCY 1 cut(s) 670
BisI GCNGC 1 cut(s) 6
BlsI GCNGC 1 cut(s) 7
Bme1390I CCNGG 3 cut(s) 122, 671, 797
Bme18I GGWCC 3 cut(s) 287, 740, 763
BmeT110I CYCGRG 1 cut(s) 785
BmgT120I GGNCC 5 cut(s) 86, 287, 582, 740, 763
BmiI GGNNCC 5 cut(s) 88, 178, 493, 583, 668
BmrFI CCNGG 3 cut(s) 122, 671, 797
BmsI GCATC 2 cut(s) 557, 833
BpiI GAAGAC 2 cut(s) 45, 689
BpmI CTGGAG 2 cut(s) 104, 192
Bpu14I TTCGAA 2 cut(s) 280, 693
BpuEI CTTGAG 1 cut(s) 124
BpuMI CCSGG 1 cut(s) 797
BsaBI GATNNNNATC 2 cut(s) 540, 596
BsaHI GRCGYC 2 cut(s) 69, 667
BsaJI CCNNGG 4 cut(s) 121, 670, 743, 796
BsaXI ACNNNNNCTCC 2 cut(s) 483, 513
Bsc4I CCNNNNNNNGG 2 cut(s) 261, 778
Bse118I RCCGGY 1 cut(s) 254
Bse3DI GCAATG 1 cut(s) 632
Bse8I GATNNNNATC 2 cut(s) 540, 596
BseBI CCWGG 2 cut(s) 122, 671
BseDI CCNNGG 4 cut(s) 121, 670, 743, 796
BseJI GATNNNNATC 2 cut(s) 540, 596
BseLI CCNNNNNNNGG 2 cut(s) 261, 778
BseMI GCAATG 1 cut(s) 632
BseMII CTCAG 2 cut(s) 380, 552
BseRI GAGGAG 1 cut(s) 44
BseYI CCCAGC 2 cut(s) 578, 832
BshFI GGCC 2 cut(s) 87, 584
BshNI GGYRCC 1 cut(s) 666
BsiHKCI CYCGRG 1 cut(s) 785
BsiSI CCGG 2 cut(s) 255, 796
BslFI GGGAC 2 cut(s) 30, 813
BslI CCNNNNNNNGG 2 cut(s) 261, 778
BsmFI GGGAC 2 cut(s) 30, 813
BsnI GGCC 2 cut(s) 87, 584
BsoBI CYCGRG 1 cut(s) 785
Bsp119I TTCGAA 2 cut(s) 280, 693
Bsp1286I GDGCHC 1 cut(s) 512
Bsp143I GATC 6 cut(s) 359, 478, 515, 541, 597, 781
BspACI CCGC 4 cut(s) 5, 414, 443, 637
BspANI GGCC 2 cut(s) 87, 584
BspCNI CTCAG 2 cut(s) 381, 553
BspLI GGNNCC 5 cut(s) 88, 178, 493, 583, 668
BspPI GGATC 2 cut(s) 367, 789
BspT104I TTCGAA 2 cut(s) 280, 693
BspT107I GGYRCC 1 cut(s) 666
BsrBI CCGCTC 1 cut(s) 414
BsrDI GCAATG 1 cut(s) 632
BsrFI RCCGGY 1 cut(s) 254
BssAI RCCGGY 1 cut(s) 254
BssECI CCNNGG 4 cut(s) 121, 670, 743, 796
BssMI GATC 6 cut(s) 359, 478, 515, 541, 597, 781
BssNI GRCGYC 2 cut(s) 69, 667
BssT1I CCWWGG 1 cut(s) 743
Bst2UI CCWGG 2 cut(s) 122, 671
Bst4CI ACNGT 2 cut(s) 44, 438
Bst6I CTCTTC 2 cut(s) 390, 761
BstACI GRCGYC 2 cut(s) 69, 667
BstBI TTCGAA 2 cut(s) 280, 693
BstC8I GCNNGC 1 cut(s) 639
BstDEI CTNAG 4 cut(s) 389, 561, 571, 808
BstH2I RGCGCY 1 cut(s) 670
BstHHI GCGC 2 cut(s) 402, 669
BstKTI GATC 6 cut(s) 362, 481, 518, 544, 600, 784
BstMBI GATC 6 cut(s) 359, 478, 515, 541, 597, 781
BstMWI GCNNNNNNNGC 1 cut(s) 491
BstNI CCWGG 2 cut(s) 122, 671
BstSCI CCNGG 3 cut(s) 120, 669, 795
BstSFI CTRYAG 1 cut(s) 471
BstV2I GAAGAC 2 cut(s) 45, 689
BstX2I RGATCY 2 cut(s) 359, 781
BstYI RGATCY 2 cut(s) 359, 781
BsuRI GGCC 2 cut(s) 87, 584
Cac8I GCNNGC 1 cut(s) 639
CfoI GCGC 2 cut(s) 402, 669
Cfr10I RCCGGY 1 cut(s) 254
Cfr13I GGNCC 5 cut(s) 86, 287, 582, 740, 763
CseI GACGC 3 cut(s) 58, 611, 810
Csp6I GTAC 1 cut(s) 10
CviAII CATG 5 cut(s) 236, 589, 688, 773, 821
CviQI GTAC 1 cut(s) 10
DdeI CTNAG 4 cut(s) 389, 561, 571, 808
DinI GGCGCC 1 cut(s) 668
DpnI GATC 6 cut(s) 361, 480, 517, 543, 599, 783
DpnII GATC 6 cut(s) 359, 478, 515, 541, 597, 781
Eam1104I CTCTTC 2 cut(s) 390, 761
EarI CTCTTC 2 cut(s) 390, 761
Eco130I CCWWGG 1 cut(s) 743
Eco24I GRGCYC 1 cut(s) 512
Eco47I GGWCC 3 cut(s) 287, 740, 763
Eco57I CTGAAG 1 cut(s) 396
Eco88I CYCGRG 1 cut(s) 785
EcoO109I RGGNCCY 1 cut(s) 86
EcoRII CCWGG 2 cut(s) 120, 669
EcoT14I CCWWGG 1 cut(s) 743
EcoT22I ATGCAT 1 cut(s) 826
EcoT38I GRGCYC 1 cut(s) 512
EgeI GGCGCC 1 cut(s) 668
EheI GGCGCC 1 cut(s) 668
ErhI CCWWGG 1 cut(s) 743
FaeI CATG 5 cut(s) 239, 592, 691, 776, 824
FalI AAGNNNNNCTT 2 cut(s) 369, 401
FaqI GGGAC 2 cut(s) 30, 813
FatI CATG 5 cut(s) 235, 588, 687, 772, 820
FauI CCCGC 1 cut(s) 630
Fnu4HI GCNGC 1 cut(s) 6
FriOI GRGCYC 1 cut(s) 512
Fsp4HI GCNGC 1 cut(s) 6
GlaI GCGC 2 cut(s) 401, 668
GluI GCNGC 1 cut(s) 6
GsaI CCCAGC 2 cut(s) 582, 836
GsuI CTGGAG 2 cut(s) 104, 192
HaeII RGCGCY 1 cut(s) 670
HaeIII GGCC 2 cut(s) 87, 584
HapII CCGG 2 cut(s) 255, 796
HgaI GACGC 3 cut(s) 58, 611, 810
HhaI GCGC 2 cut(s) 402, 669
Hin1I GRCGYC 2 cut(s) 69, 667
Hin1II CATG 5 cut(s) 239, 592, 691, 776, 824
Hin6I GCGC 2 cut(s) 400, 667
HinP1I GCGC 2 cut(s) 400, 667
HindIII AAGCTT 1 cut(s) 696
HinfI GANTC 3 cut(s) 134, 629, 751
HpaII CCGG 2 cut(s) 255, 796
HphI GGTGA 3 cut(s) 221, 488, 760
Hpy166II GTNNAC 2 cut(s) 620, 763
Hpy188I TCNGA 8 cut(s) 181, 390, 423, 463, 507, 523, 549, 718
Hpy188III TCNNGA 6 cut(s) 47, 103, 171, 365, 374, 785
Hpy8I GTNNAC 2 cut(s) 620, 763
Hpy99I CGWCG 2 cut(s) 295, 605
HpyAV CCTTC 2 cut(s) 549, 649
HpyCH4III ACNGT 2 cut(s) 44, 438
HpyCH4IV ACGT 2 cut(s) 114, 498
HpyCH4V TGCA 1 cut(s) 824
HpyF10VI GCNNNNNNNGC 1 cut(s) 491
HpyF3I CTNAG 4 cut(s) 389, 561, 571, 808
HpySE526I ACGT 2 cut(s) 114, 498
Hsp92I GRCGYC 2 cut(s) 69, 667
Hsp92II CATG 5 cut(s) 239, 592, 691, 776, 824
HspAI GCGC 2 cut(s) 400, 667
KasI GGCGCC 1 cut(s) 666
Kzo9I GATC 6 cut(s) 359, 478, 515, 541, 597, 781
LmnI GCTCC 1 cut(s) 491
LweI GCATC 2 cut(s) 557, 833
MaeII ACGT 2 cut(s) 114, 498
MaeIII GTNAC 2 cut(s) 70, 214
MalI GATC 6 cut(s) 361, 480, 517, 543, 599, 783
MbiI CCGCTC 1 cut(s) 414
MboI GATC 6 cut(s) 359, 478, 515, 541, 597, 781
MboII GAAGA 8 cut(s) 50, 377, 389, 536, 545, 694, 748, 831
MflI RGATCY 2 cut(s) 359, 781
MhlI GDGCHC 1 cut(s) 512
MluCI AATT 3 cut(s) 271, 524, 648
Mly113I GGCGCC 1 cut(s) 667
MlyI GAGTC 2 cut(s) 143, 623
MmeI TCCRAC 2 cut(s) 204, 486
MnlI CCTC 9 cut(s) 22, 66, 77, 110, 128, 167, 307, 568, 803
Mph1103I ATGCAT 1 cut(s) 826
MroXI GAANNNNTTC 4 cut(s) 23, 200, 384, 755
MseI TTAA 4 cut(s) 188, 408, 453, 856
MslI CAYNNNNRTG 5 cut(s) 77, 234, 593, 718, 771
MspI CCGG 2 cut(s) 255, 796
MspR9I CCNGG 3 cut(s) 122, 671, 797
MvaI CCWGG 2 cut(s) 122, 671
MwoI GCNNNNNNNGC 1 cut(s) 491
NarI GGCGCC 1 cut(s) 667
NciI CCSGG 1 cut(s) 797
NdeII GATC 6 cut(s) 359, 478, 515, 541, 597, 781
NlaIII CATG 5 cut(s) 239, 592, 691, 776, 824
NlaIV GGNNCC 5 cut(s) 88, 178, 493, 583, 668
NmuCI GTSAC 2 cut(s) 70, 214
NsiI ATGCAT 1 cut(s) 826
NspV TTCGAA 2 cut(s) 280, 693
PaeR7I CTCGAG 1 cut(s) 785
PcsI WCGNNNNNNNCGW 1 cut(s) 33
PdmI GAANNNNTTC 4 cut(s) 23, 200, 384, 755
PfeI GAWTC 1 cut(s) 751
PflMI CCANNNNNTGG 1 cut(s) 778
PkrI GCNGC 1 cut(s) 7
PleI GAGTC 2 cut(s) 142, 623
PluTI GGCGCC 1 cut(s) 670
PpsI GAGTC 2 cut(s) 142, 623
Psp6I CCWGG 2 cut(s) 120, 669
PspFI CCCAGC 2 cut(s) 578, 832
PspGI CCWGG 2 cut(s) 120, 669
PspN4I GGNNCC 5 cut(s) 88, 178, 493, 583, 668
PspPI GGNCC 5 cut(s) 86, 287, 582, 740, 763
PsuI RGATCY 2 cut(s) 359, 781
RsaI GTAC 1 cut(s) 11
RsaNI GTAC 1 cut(s) 10
RseI CAYNNNNRTG 5 cut(s) 77, 234, 593, 718, 771
SaqAI TTAA 4 cut(s) 188, 408, 453, 856
SatI GCNGC 1 cut(s) 6
Sau3AI GATC 6 cut(s) 359, 478, 515, 541, 597, 781
Sau96I GGNCC 5 cut(s) 86, 287, 582, 740, 763
SchI GAGTC 2 cut(s) 143, 623
ScrFI CCNGG 3 cut(s) 122, 671, 797
SduI GDGCHC 1 cut(s) 512
SfaNI GCATC 2 cut(s) 557, 833
SfcI CTRYAG 1 cut(s) 471
SfoI GGCGCC 1 cut(s) 668
Sfr274I CTCGAG 1 cut(s) 785
SfuI TTCGAA 2 cut(s) 280, 693
SgrAI CRCCGGYG 1 cut(s) 254
SinI GGWCC 3 cut(s) 287, 740, 763
SlaI CTCGAG 1 cut(s) 785
SmiMI CAYNNNNRTG 5 cut(s) 77, 234, 593, 718, 771
SmlI CTYRAG 2 cut(s) 103, 785
SmoI CTYRAG 2 cut(s) 103, 785
Sse9I AATT 3 cut(s) 271, 524, 648
SsiI CCGC 4 cut(s) 5, 414, 443, 637
SspDI GGCGCC 1 cut(s) 666
StyD4I CCNGG 3 cut(s) 120, 669, 795
StyI CCWWGG 1 cut(s) 743
TaaI ACNGT 2 cut(s) 44, 438
TaiI ACGT 2 cut(s) 117, 501
TaqI TCGA 8 cut(s) 27, 58, 280, 345, 481, 600, 693, 786
TasI AATT 3 cut(s) 271, 524, 648
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 1 cut(s) 751
Tru1I TTAA 4 cut(s) 188, 408, 453, 856
Tru9I TTAA 4 cut(s) 188, 408, 453, 856
TseFI GTSAC 2 cut(s) 70, 214
Tsp45I GTSAC 2 cut(s) 70, 214
TspDTI ATGAA 2 cut(s) 193, 715
TspGWI ACGGA 1 cut(s) 279
Van91I CCANNNNNTGG 1 cut(s) 778
VpaK11BI GGWCC 3 cut(s) 287, 740, 763
XapI RAATTY 2 cut(s) 271, 524
XhoI CTCGAG 1 cut(s) 785
XmnI GAANNNNTTC 4 cut(s) 23, 200, 384, 755
Zsp2I ATGCAT 1 cut(s) 826
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.