pycom05g30710

(2R)-phospho-3-sulfolactate synthase (ComA)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0007662
Physical Location & Seq
Reverse (-)
30734407 .. 30735090
684 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g30710.1

Sequence Viewer

Length: 417 bp
ATGGTCATTTTTTGGGAGATTAGAGTGGCTAGGGAATGTAAGAGCTTGGGGTTTGACACGATCGAGCTGAATGAGGGATCTCTTGAACTTCCCGAAGAAATTCTTCTGAGATTCGTTCGCTTGATTAAGATAGGTGGTCTGAAAGTGAAGTCACAGTTTGCGATCAGGTTTAACAAGTCTGACATACCTGTAGGTGACAGCATTTGGAGCCTATGTTATCTCGAGCCCACGATATGGTATGCTGTGCAATCTTTCCTGAAACAATTAGTGATTGATATGTTCGCCTTGATACCTAGATATTGTCGGAAGAGCCGCATAGAAATTGTAGATTTTCTTATTGGGGCATTTAATAGCTCCAAGCTTAAAGAACTTAATCGCCACTCTTCGTTCTTTTGTTCAGCTGTTATGCGAAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0008150 GO:0009266 GO:0009408 GO:0009628 GO:0010286 GO:0010468 GO:0010608 GO:0019222 GO:0050789 GO:0050896 GO:0060255 GO:0065007
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ComA

Protein Analysis

139

Amino Acids

16.1

Weight (kDa)

9.18

Isoelectric Point (pI)

45.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ComA PF02679 10 - 63 1e-09 (2R)-phospho-3-sulfolactate synthase (ComA)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 234
AciI CCGC 1 cut(s) 313
AclWI GGATC 1 cut(s) 85
AcsI RAATTY 1 cut(s) 99
AfiI CCNNNNNNNGG 1 cut(s) 234
AgsI TTSAA 1 cut(s) 86
AluBI AGCT 5 cut(s) 45, 67, 354, 361, 401
AluI AGCT 5 cut(s) 45, 67, 354, 361, 401
AlwI GGATC 1 cut(s) 85
Ama87I CYCGRG 1 cut(s) 221
ApoI RAATTY 1 cut(s) 99
Asp700I GAANNNNTTC 2 cut(s) 99, 102
AsuHPI GGTGA 1 cut(s) 206
AvaI CYCGRG 1 cut(s) 221
BanII GRGCYC 1 cut(s) 228
BfaI CTAG 2 cut(s) 30, 294
BfmI CTRYAG 1 cut(s) 189
BisI GCNGC 1 cut(s) 313
BlsI GCNGC 1 cut(s) 314
BmeT110I CYCGRG 1 cut(s) 221
BmiI GGNNCC 1 cut(s) 209
BsaXI ACNNNNNCTCC 2 cut(s) 199, 229
Bsc4I CCNNNNNNNGG 1 cut(s) 234
BseLI CCNNNNNNNGG 1 cut(s) 234
BseMII CTCAG 1 cut(s) 98
Bsh1285I CGRYCG 1 cut(s) 63
BsiEI CGRYCG 1 cut(s) 63
BsiHKCI CYCGRG 1 cut(s) 221
BslI CCNNNNNNNGG 1 cut(s) 234
BsoBI CYCGRG 1 cut(s) 221
Bsp1286I GDGCHC 1 cut(s) 228
Bsp143I GATC 3 cut(s) 60, 77, 162
BspACI CCGC 1 cut(s) 313
BspCNI CTCAG 1 cut(s) 99
BspLI GGNNCC 1 cut(s) 209
BspPI GGATC 1 cut(s) 85
BspQI GCTCTTC 1 cut(s) 302
BssMI GATC 3 cut(s) 60, 77, 162
Bst4CI ACNGT 1 cut(s) 156
Bst6I CTCTTC 2 cut(s) 302, 388
BstDEI CTNAG 1 cut(s) 107
BstKTI GATC 3 cut(s) 63, 80, 165
BstMBI GATC 3 cut(s) 60, 77, 162
BstMCI CGRYCG 1 cut(s) 63
BstMWI GCNNNNNNNGC 1 cut(s) 207
BstSFI CTRYAG 1 cut(s) 189
BstX2I RGATCY 1 cut(s) 77
BstYI RGATCY 1 cut(s) 77
CviJI RGCY 9 cut(s) 29, 45, 67, 210, 226, 312, 354, 361, 401
CviKI_1 RGCY 9 cut(s) 29, 45, 67, 210, 226, 312, 354, 361, 401
DdeI CTNAG 1 cut(s) 107
DpnI GATC 3 cut(s) 62, 79, 164
DpnII GATC 3 cut(s) 60, 77, 162
Eam1104I CTCTTC 2 cut(s) 302, 388
EarI CTCTTC 2 cut(s) 302, 388
Eco24I GRGCYC 1 cut(s) 228
Eco88I CYCGRG 1 cut(s) 221
EcoT38I GRGCYC 1 cut(s) 228
FaiI YATR 7 cut(s) 185, 214, 235, 240, 278, 317, 407
FalI AAGNNNNNCTT 2 cut(s) 87, 119
Fnu4HI GCNGC 1 cut(s) 313
FriOI GRGCYC 1 cut(s) 228
Fsp4HI GCNGC 1 cut(s) 313
FspBI CTAG 2 cut(s) 30, 294
GluI GCNGC 1 cut(s) 313
HindIII AAGCTT 1 cut(s) 359
HinfI GANTC 1 cut(s) 111
HphI GGTGA 1 cut(s) 206
Hpy188I TCNGA 4 cut(s) 108, 141, 181, 306
Hpy188III TCNNGA 4 cut(s) 83, 92, 221, 256
HpyAV CCTTC 1 cut(s) 405
HpyCH4III ACNGT 1 cut(s) 156
HpyCH4V TGCA 1 cut(s) 247
HpyF10VI GCNNNNNNNGC 1 cut(s) 207
HpyF3I CTNAG 1 cut(s) 107
Kzo9I GATC 3 cut(s) 60, 77, 162
LguI GCTCTTC 1 cut(s) 302
LmnI GCTCC 2 cut(s) 207, 359
LpnPI CCDG 3 cut(s) 151, 201, 269
MaeI CTAG 2 cut(s) 30, 294
MaeIII GTNAC 2 cut(s) 150, 194
MalI GATC 3 cut(s) 62, 79, 164
MboI GATC 3 cut(s) 60, 77, 162
MboII GAAGA 4 cut(s) 95, 107, 319, 375
MflI RGATCY 1 cut(s) 77
MhlI GDGCHC 1 cut(s) 228
MluCI AATT 3 cut(s) 99, 263, 321
MmeI TCCRAC 1 cut(s) 284
MnlI CCTC 1 cut(s) 67
MroXI GAANNNNTTC 2 cut(s) 99, 102
MseI TTAA 5 cut(s) 126, 171, 348, 363, 372
MspA1I CMGCKG 1 cut(s) 401
MwoI GCNNNNNNNGC 1 cut(s) 207
NdeII GATC 3 cut(s) 60, 77, 162
NlaIV GGNNCC 1 cut(s) 209
NmuCI GTSAC 2 cut(s) 150, 194
PaeR7I CTCGAG 1 cut(s) 221
PciSI GCTCTTC 1 cut(s) 302
PdmI GAANNNNTTC 2 cut(s) 99, 102
PfeI GAWTC 1 cut(s) 111
PflMI CCANNNNNTGG 1 cut(s) 234
PkrI GCNGC 1 cut(s) 314
Ple19I CGATCG 1 cut(s) 63
PspN4I GGNNCC 1 cut(s) 209
PsuI RGATCY 1 cut(s) 77
PvuI CGATCG 1 cut(s) 63
PvuII CAGCTG 1 cut(s) 401
SapI GCTCTTC 1 cut(s) 302
SaqAI TTAA 5 cut(s) 126, 171, 348, 363, 372
SatI GCNGC 1 cut(s) 313
Sau3AI GATC 3 cut(s) 60, 77, 162
SduI GDGCHC 1 cut(s) 228
SfcI CTRYAG 1 cut(s) 189
Sfr274I CTCGAG 1 cut(s) 221
SlaI CTCGAG 1 cut(s) 221
SmlI CTYRAG 1 cut(s) 221
SmoI CTYRAG 1 cut(s) 221
Sse9I AATT 3 cut(s) 99, 263, 321
SsiI CCGC 1 cut(s) 313
SspMI CTAG 2 cut(s) 30, 294
TaaI ACNGT 1 cut(s) 156
TaqI TCGA 2 cut(s) 63, 222
TasI AATT 3 cut(s) 99, 263, 321
TauI GCSGC 1 cut(s) 315
TfiI GAWTC 1 cut(s) 111
Tru1I TTAA 5 cut(s) 126, 171, 348, 363, 372
Tru9I TTAA 5 cut(s) 126, 171, 348, 363, 372
TseFI GTSAC 2 cut(s) 150, 194
Tsp45I GTSAC 2 cut(s) 150, 194
Van91I CCANNNNNTGG 1 cut(s) 234
XapI RAATTY 1 cut(s) 99
XhoI CTCGAG 1 cut(s) 221
XmnI GAANNNNTTC 2 cut(s) 99, 102
XspI CTAG 2 cut(s) 30, 294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.