Rorug04G0412400

(2R)-phospho-3-sulfolactate synthase (ComA)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0007662
Physical Location & Seq
Reverse (-)
57443120 .. 57447512
4393 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0412400.1

Sequence Viewer

Length: 1971 bp
ATGATTTCCTGGAACGACCTCTACAAGGTCTTGACGGCGGTGGTGCCTCTCTACGTGGCCATGATCTTAGCCTACGGCTCCGTCCGGTGGTGGAAGATCTTCTCCCCGGACCAATGCTCCGGCATCAACCGCTTCGTCGCAATCTTCGCAGTTCCTCTTCTCTCCTTCCACTTCATCTCCACCAACGACCCTTACACCATGAACTTCCGCTTCATCGCCGCCGACACTCTCCAGAAGATCATCATGCTCTTCGCCCTGGCCCTCTGGACCAACTTCACCCGAAACGGAAGCCTCGAGTGGATGATCACGATTTTCTCGCTCTCCACGCTTCCCAATACTCTGGTCATGGGAATCCCCCTGCTGATCGCCATGTACGGCCACCGAATCCCCGAGTCCGGCAGCCTGATGGTCCAGGTCGTCGTGCTCCAGTGCATCATTTGGTACACTCTGTTACTCTTCCTCTTCGAGTACCGCGGCGCCAAGCTGCTGATAATGGAGCAGTTCCCGGAGACCGCCGCCTCCATCGTCTCCTTCAAGGTCGACTCCGACGTCATGTCCCTCGACGGCCGCGACTTCCTCGAAACCGACGCCGAGATCGGCGAAGACGGAAAGCTCCACGTCAAGGTGAGGAAGTCCAACGCGTCCAGACGGTCGCTCTCGGCAATGACTCCTCGGCCGTCCAACCTCACCGGAGCCGAGATTTACAGCCTCAGCTCTTCCCGGAATCCCACTCCCCGAGGCTCCAATTTCAACCCCTCCGATTTTTACTCCGTGATGGGAGTCCAGGGCTTCAATACCCGCCACTCCAATTTCGGACCCGCCGACTTGTACTCGGTTCAGTCCTCCCGAGGTCCGACTCCCAGACCCTCCAATTTCGAAGAGAATTCTGCTCCAATATTGGCGCAGACGGTTACTTCTCCCCGGTTCGGGTTTTACCCGGCCCAGACTGTCCCCTCGTCGTACCCGGCTCCGAATCCGGAATTCTCCGCCCCCGTCACTAAAAATAACCAACAGCAACCCCAACCGCCGCCGCAGCAGCAGGTTAACTCCAACAGTGGCAAAGCGAATCACGACGCGAAAGAGCTCCACATGTTTGTTTGGAGCTCCAGCAATTCCCCTGTTTCCGAAGGCGGAGGGCTCCACGTTTTCGGCGGACCTGACTTCGCCGCGTCGGAGCAATCGGGGCGGTCCGATCAGGGCGCCAAGGAGATCAGGATGTTGGTTGCTGACCACCCCCAGAATGGAGAAACCAAAGTGGCGGTTGCGGAGAGTGACGGCGATCACTACCACCACGGCGGAGAAGCCTTCACGTTCACCGGAAAGGCTGATCAGGGAGAGGAGGAGAGAGAAAAGGAGGGGCCAACCGGACTTAACAAGCAGGGGTCAAGCTCCACTGCCGAGCTACACGGCGGGAAAGGTGGAGGAGGAGTGGGGGTTGATCACGAGTCAAGCGGCGGAAAACAAATGCCTCCGGCGAGCGTTATGACGCGTCTGATCTTGATCATGGTTTGGCGGAAGCTTATCCGCAATCCCAACACGTACTCTAGCCTCATCGGTCTCATCTGGTCGCTCATCGCTTTCCGGTGGGACGTTGCTATGCCAAAAATAATAGAGAAGTCGATCTCCATACTGTCCGATGCTGGGCTAGGAATGGCTATGTTCAGTTTAGGTCTTTTTATGGCACTTCAACCTAAGATAATAGCATGTGGAAACTCGGTGGCCGGGTTTGCAATGGCCGTGAGATTCCTCACTGGCCCGGCTGTGATGGCTGCAGCCTCCATTGCTGTTGGGCTACGTGGCACCCTCCTCCACTTAGCTATTGTCCAGGCTGCGCTTCCACAAGGAATTGTTCCCTTTGTCTTTGCCAAGGAGTACAATGTTCATCCATCCATTCTTAGCACTGCGGTTATATTCGGGATGTTGATAGCATTACCGATTACGCTGGTTTACTATATCGTTCTCGGACTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ComA

Protein Analysis

656

Amino Acids

71.36

Weight (kDa)

6.96

Isoelectric Point (pI)

39.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mem_trans PF03547 10 - 651 6.8e-128 Membrane transport protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 548
AatII GACGTC 1 cut(s) 552
Acc36I ACCTGC 1 cut(s) 1030
AccB1I GGYRCC 4 cut(s) 43, 476, 1199, 1799
AccI GTMKAC 1 cut(s) 540
AccII CGCG 6 cut(s) 474, 570, 641, 1076, 1169, 1489
AccIII TCCGGA 1 cut(s) 976
AcoI YGGCCR 6 cut(s) 57, 376, 565, 674, 1719, 1734
AcsI RAATTY 2 cut(s) 883, 980
AcyI GRCGYC 4 cut(s) 477, 549, 588, 1200
AfaI GTAC 7 cut(s) 374, 443, 470, 830, 962, 1541, 1874
AfiI CCNNNNNNNGG 8 cut(s) 25, 87, 395, 622, 926, 927, 1241, 1641
AflIII ACRYGT 4 cut(s) 639, 1089, 1487, 1536
AgsI TTSAA 4 cut(s) 535, 751, 793, 1688
AhdI GACNNNNNGTC 1 cut(s) 553
AjiI CACGTC 1 cut(s) 619
AjnI CCWGG 5 cut(s) 8, 255, 411, 783, 1824
AluBI AGCT 9 cut(s) 484, 613, 714, 1084, 1104, 1389, 1402, 1519, 1817
AluI AGCT 9 cut(s) 484, 613, 714, 1084, 1104, 1389, 1402, 1519, 1817
Alw21I GWGCWC 3 cut(s) 426, 1086, 1106
Alw26I GTCTC 3 cut(s) 503, 532, 1562
Ama87I CYCGRG 4 cut(s) 293, 389, 735, 846
Aor13HI TCCGGA 1 cut(s) 976
ApeKI GCWGC 7 cut(s) 399, 484, 1033, 1036, 1769, 1772, 1829
ApoI RAATTY 2 cut(s) 883, 980
Asp700I GAANNNNTTC 1 cut(s) 98
AspLEI GCGC 4 cut(s) 479, 904, 1202, 1834
AsuC2I CCSGG 8 cut(s) 107, 506, 721, 922, 938, 965, 1723, 1757
AsuHPI GGTGA 4 cut(s) 268, 637, 679, 1306
AsuII TTCGAA 1 cut(s) 876
AvaI CYCGRG 4 cut(s) 293, 389, 735, 846
AvaII GGWCC 7 cut(s) 109, 267, 409, 815, 851, 1154, 1188
BalI TGGCCA 1 cut(s) 59
BanI GGYRCC 4 cut(s) 43, 476, 1199, 1799
BanII GRGCYC 3 cut(s) 1086, 1106, 1140
BauI CACGAG 1 cut(s) 1442
BbsI GAAGAC 1 cut(s) 609
Bbv12I GWGCWC 3 cut(s) 426, 1086, 1106
BbvCI CCTCAGC 1 cut(s) 710
BbvI GCAGC 7 cut(s) 411, 471, 1045, 1048, 1756, 1784, 1816
BccI CCATC 5 cut(s) 400, 530, 769, 1759, 1894
BceAI ACGGC 9 cut(s) 51, 91, 391, 580, 661, 1291, 1309, 1423, 1721
BciT130I CCWGG 5 cut(s) 10, 257, 413, 785, 1826
BclI TGATCA 4 cut(s) 303, 1327, 1438, 1500
BcnI CCSGG 8 cut(s) 107, 506, 721, 922, 938, 965, 1723, 1757
BcoDI GTCTC 3 cut(s) 503, 532, 1562
BfaI CTAG 2 cut(s) 1545, 1646
BfmI CTRYAG 1 cut(s) 1770
BfoI RGCGCY 2 cut(s) 480, 1203
BfuAI ACCTGC 1 cut(s) 1030
BglII AGATCT 1 cut(s) 96
Bme18I GGWCC 7 cut(s) 109, 267, 409, 815, 851, 1154, 1188
BmeRI GACNNNNNGTC 1 cut(s) 553
BmeT110I CYCGRG 4 cut(s) 293, 389, 735, 846
BmgBI CACGTC 1 cut(s) 619
BmsI GCATC 3 cut(s) 132, 441, 1627
BpiI GAAGAC 1 cut(s) 609
BpmI CTGGAG 3 cut(s) 215, 410, 1090
Bpu10I CCTNAGC 1 cut(s) 710
Bpu14I TTCGAA 1 cut(s) 876
BpuMI CCSGG 8 cut(s) 107, 506, 721, 922, 938, 965, 1723, 1757
BsaAI YACGTR 3 cut(s) 55, 1539, 1796
BsaBI GATNNNNATC 1 cut(s) 1499
BsaHI GRCGYC 4 cut(s) 477, 549, 588, 1200
BsaI GGTCTC 2 cut(s) 503, 1562
BsaWI WCCGGW 6 cut(s) 84, 689, 976, 1316, 1364, 1581
BsaXI ACNNNNNCTCC 8 cut(s) 101, 131, 161, 191, 1413, 1419, 1443, 1449
Bsc4I CCNNNNNNNGG 8 cut(s) 25, 87, 395, 622, 926, 927, 1241, 1641
Bse1I ACTGG 2 cut(s) 427, 1756
Bse3DI GCAATG 3 cut(s) 669, 1737, 1779
Bse8I GATNNNNATC 1 cut(s) 1499
BseAI TCCGGA 1 cut(s) 976
BseBI CCWGG 5 cut(s) 10, 257, 413, 785, 1826
BseGI GGATG 5 cut(s) 306, 1221, 1882, 1886, 1923
BseJI GATNNNNATC 1 cut(s) 1499
BseLI CCNNNNNNNGG 8 cut(s) 25, 87, 395, 622, 926, 927, 1241, 1641
BseMI GCAATG 3 cut(s) 669, 1737, 1779
BseMII CTCAG 1 cut(s) 724
BseNI ACTGG 2 cut(s) 427, 1756
BseRI GAGGAG 6 cut(s) 660, 1352, 1355, 1437, 1440, 1796
BseX3I CGGCCG 2 cut(s) 565, 674
BseXI GCAGC 7 cut(s) 411, 471, 1045, 1048, 1756, 1784, 1816
BseYI CCCAGC 1 cut(s) 1640
Bsh1236I CGCG 6 cut(s) 474, 570, 641, 1076, 1169, 1489
Bsh1285I CGRYCG 3 cut(s) 568, 653, 677
BshNI GGYRCC 4 cut(s) 43, 476, 1199, 1799
BsiEI CGRYCG 3 cut(s) 568, 653, 677
BsiHKAI GWGCWC 3 cut(s) 426, 1086, 1106
BsiHKCI CYCGRG 4 cut(s) 293, 389, 735, 846
BslFI GGGAC 3 cut(s) 541, 935, 1601
BslI CCNNNNNNNGG 8 cut(s) 25, 87, 395, 622, 926, 927, 1241, 1641
BsmAI GTCTC 3 cut(s) 503, 532, 1562
BsmBI CGTCTC 1 cut(s) 532
BsmFI GGGAC 3 cut(s) 541, 935, 1601
Bso31I GGTCTC 2 cut(s) 503, 1562
BsoBI CYCGRG 4 cut(s) 293, 389, 735, 846
Bsp119I TTCGAA 1 cut(s) 876
Bsp1286I GDGCHC 4 cut(s) 426, 1086, 1106, 1140
Bsp13I TCCGGA 1 cut(s) 976
BspCNI CTCAG 1 cut(s) 723
BspEI TCCGGA 1 cut(s) 976
BspFNI CGCG 6 cut(s) 474, 570, 641, 1076, 1169, 1489
BspMAI CTGCAG 1 cut(s) 1774
BspMI ACCTGC 1 cut(s) 1030
BspQI GCTCTTC 2 cut(s) 254, 721
BspT104I TTCGAA 1 cut(s) 876
BspT107I GGYRCC 4 cut(s) 43, 476, 1199, 1799
BspTNI GGTCTC 2 cut(s) 503, 1562
BsrDI GCAATG 3 cut(s) 669, 1737, 1779
BsrI ACTGG 2 cut(s) 427, 1756
BssNI GRCGYC 4 cut(s) 477, 549, 588, 1200
BssSI CACGAG 1 cut(s) 1442
BssT1I CCWWGG 2 cut(s) 1203, 1866
Bst2BI CACGAG 1 cut(s) 1442
Bst2UI CCWGG 5 cut(s) 10, 257, 413, 785, 1826
Bst4CI ACNGT 6 cut(s) 651, 910, 949, 1055, 1632, 1968
Bst6I CTCTTC 6 cut(s) 162, 254, 461, 467, 721, 873
BstACI GRCGYC 4 cut(s) 477, 549, 588, 1200
BstBAI YACGTR 3 cut(s) 55, 1539, 1796
BstBI TTCGAA 1 cut(s) 876
BstC8I GCNNGC 1 cut(s) 1477
BstDEI CTNAG 5 cut(s) 67, 710, 1692, 1813, 1895
BstDSI CCRYGG 2 cut(s) 472, 1291
BstENI CCTNNNNNAGG 1 cut(s) 23
BstF5I GGATG 5 cut(s) 306, 1221, 1882, 1886, 1923
BstFNI CGCG 6 cut(s) 474, 570, 641, 1076, 1169, 1489
BstH2I RGCGCY 2 cut(s) 480, 1203
BstHHI GCGC 4 cut(s) 479, 904, 1202, 1834
BstMAI GTCTC 3 cut(s) 503, 532, 1562
BstMCI CGRYCG 3 cut(s) 568, 653, 677
BstMWI GCNNNNNNNGC 9 cut(s) 129, 146, 325, 1033, 1036, 1183, 1727, 1766, 1781
BstNI CCWGG 5 cut(s) 10, 257, 413, 785, 1826
BstNSI RCATGY 2 cut(s) 1093, 1707
BstSFI CTRYAG 1 cut(s) 1770
BstUI CGCG 6 cut(s) 474, 570, 641, 1076, 1169, 1489
BstV1I GCAGC 7 cut(s) 411, 471, 1045, 1048, 1756, 1784, 1816
BstV2I GAAGAC 1 cut(s) 609
BstX2I RGATCY 1 cut(s) 96
BstXI CCANNNNNNTGG 1 cut(s) 340
BstYI RGATCY 1 cut(s) 96
BstZI CGGCCG 2 cut(s) 565, 674
BtgI CCRYGG 2 cut(s) 472, 1291
BtgZI GCGATG 2 cut(s) 199, 1558
BtrI CACGTC 1 cut(s) 619
BtsCI GGATG 5 cut(s) 306, 1221, 1882, 1886, 1923
BtsI GCAGTG 2 cut(s) 1392, 1899
BtsIMutI CAGTG 5 cut(s) 434, 1060, 1392, 1749, 1899
BveI ACCTGC 1 cut(s) 1030
Cac8I GCNNGC 1 cut(s) 1477
CfoI GCGC 4 cut(s) 479, 904, 1202, 1834
Cfr42I CCGCGG 1 cut(s) 475
CpoI CGGWCCG 1 cut(s) 1188
CseI GACGC 6 cut(s) 596, 630, 1082, 1158, 1478, 1495
Csp6I GTAC 7 cut(s) 373, 442, 469, 829, 961, 1540, 1873
CspI CGGWCCG 1 cut(s) 1188
CviAII CATG 9 cut(s) 61, 199, 244, 346, 370, 553, 1090, 1504, 1704
CviQI GTAC 7 cut(s) 373, 442, 469, 829, 961, 1540, 1873
DdeI CTNAG 5 cut(s) 67, 710, 1692, 1813, 1895
DinI GGCGCC 2 cut(s) 478, 1201
DrdI GACNNNNNNGTC 1 cut(s) 548
DriI GACNNNNNGTC 1 cut(s) 553
DseDI GACNNNNNNGTC 1 cut(s) 548
EaeI YGGCCR 6 cut(s) 57, 376, 565, 674, 1719, 1734
EagI CGGCCG 2 cut(s) 565, 674
Eam1104I CTCTTC 6 cut(s) 162, 254, 461, 467, 721, 873
Eam1105I GACNNNNNGTC 1 cut(s) 553
EarI CTCTTC 6 cut(s) 162, 254, 461, 467, 721, 873
EciI GGCGGA 6 cut(s) 976, 1146, 1167, 1311, 1470, 1528
Ecl136II GAGCTC 2 cut(s) 1084, 1104
EclXI CGGCCG 2 cut(s) 565, 674
Eco130I CCWWGG 2 cut(s) 1203, 1866
Eco24I GRGCYC 3 cut(s) 1086, 1106, 1140
Eco31I GGTCTC 2 cut(s) 503, 1562
Eco47I GGWCC 7 cut(s) 109, 267, 409, 815, 851, 1154, 1188
Eco52I CGGCCG 2 cut(s) 565, 674
Eco53kI GAGCTC 2 cut(s) 1084, 1104
Eco88I CYCGRG 4 cut(s) 293, 389, 735, 846
EcoICRI GAGCTC 2 cut(s) 1084, 1104
EcoNI CCTNNNNNAGG 1 cut(s) 23
EcoRI GAATTC 2 cut(s) 883, 980
EcoRII CCWGG 5 cut(s) 8, 255, 411, 783, 1824
EcoT14I CCWWGG 2 cut(s) 1203, 1866
EcoT38I GRGCYC 3 cut(s) 1086, 1106, 1140
EgeI GGCGCC 2 cut(s) 478, 1201
EheI GGCGCC 2 cut(s) 478, 1201
ErhI CCWWGG 2 cut(s) 1203, 1866
Esp3I CGTCTC 1 cut(s) 532
FaeI CATG 9 cut(s) 64, 202, 247, 349, 373, 556, 1093, 1507, 1707
FaqI GGGAC 3 cut(s) 541, 935, 1601
FatI CATG 9 cut(s) 60, 198, 243, 345, 369, 552, 1089, 1503, 1703
FauI CCCGC 3 cut(s) 806, 826, 1403
FbaI TGATCA 4 cut(s) 303, 1327, 1438, 1500
FblI GTMKAC 1 cut(s) 540
FokI GGATG 5 cut(s) 313, 1228, 1869, 1873, 1930
FriOI GRGCYC 3 cut(s) 1086, 1106, 1140
FspBI CTAG 2 cut(s) 1545, 1646
GlaI GCGC 4 cut(s) 478, 903, 1201, 1833
GsaI CCCAGC 1 cut(s) 1644
GsuI CTGGAG 3 cut(s) 215, 410, 1090
HaeII RGCGCY 2 cut(s) 480, 1203
HgaI GACGC 6 cut(s) 596, 630, 1082, 1158, 1478, 1495
HhaI GCGC 4 cut(s) 479, 904, 1202, 1834
Hin1I GRCGYC 4 cut(s) 477, 549, 588, 1200
Hin1II CATG 9 cut(s) 64, 202, 247, 349, 373, 556, 1093, 1507, 1707
Hin6I GCGC 4 cut(s) 477, 902, 1200, 1832
HinP1I GCGC 4 cut(s) 477, 902, 1200, 1832
HincII GTYRAC 2 cut(s) 541, 1045
HindII GTYRAC 2 cut(s) 541, 1045
HindIII AAGCTT 1 cut(s) 1517
HpaI GTTAAC 1 cut(s) 1045
HphI GGTGA 4 cut(s) 268, 637, 679, 1306
Hpy166II GTNNAC 5 cut(s) 444, 541, 1045, 1314, 1948
Hpy8I GTNNAC 5 cut(s) 444, 541, 1045, 1314, 1948
Hpy99I CGWCG 8 cut(s) 140, 422, 551, 566, 590, 961, 1076, 1174
HpyAV CCTTC 4 cut(s) 175, 541, 1121, 1315
HpyCH4III ACNGT 6 cut(s) 651, 910, 949, 1055, 1632, 1968
HpyCH4IV ACGT 8 cut(s) 54, 549, 618, 1143, 1310, 1538, 1590, 1795
HpyCH4V TGCA 3 cut(s) 432, 1730, 1772
HpyF10VI GCNNNNNNNGC 9 cut(s) 129, 146, 325, 1033, 1036, 1183, 1727, 1766, 1781
HpyF3I CTNAG 5 cut(s) 67, 710, 1692, 1813, 1895
HpySE526I ACGT 8 cut(s) 54, 549, 618, 1143, 1310, 1538, 1590, 1795
Hsp92I GRCGYC 4 cut(s) 477, 549, 588, 1200
Hsp92II CATG 9 cut(s) 64, 202, 247, 349, 373, 556, 1093, 1507, 1707
HspAI GCGC 4 cut(s) 477, 902, 1200, 1832
KasI GGCGCC 2 cut(s) 476, 1199
Kpn2I TCCGGA 1 cut(s) 976
Ksp22I TGATCA 4 cut(s) 303, 1327, 1438, 1500
KspAI GTTAAC 1 cut(s) 1045
KspI CCGCGG 1 cut(s) 475
LguI GCTCTTC 2 cut(s) 254, 721
Lsp1109I GCAGC 7 cut(s) 411, 471, 1045, 1048, 1756, 1784, 1816
LweI GCATC 3 cut(s) 132, 441, 1627
MaeI CTAG 2 cut(s) 1545, 1646
MaeII ACGT 8 cut(s) 54, 549, 618, 1143, 1310, 1538, 1590, 1795
MaeIII GTNAC 4 cut(s) 450, 910, 994, 1271
MflI RGATCY 1 cut(s) 96
MhlI GDGCHC 4 cut(s) 426, 1086, 1106, 1140
MlsI TGGCCA 1 cut(s) 59
MluCI AATT 7 cut(s) 745, 808, 871, 883, 980, 1111, 1845
MluI ACGCGT 2 cut(s) 639, 1487
MluNI TGGCCA 1 cut(s) 59
Mly113I GGCGCC 2 cut(s) 477, 1200
MlyI GAGTC 6 cut(s) 401, 536, 661, 789, 850, 1454
MmeI TCCRAC 6 cut(s) 570, 660, 705, 878, 1074, 1152
Mox20I TGGCCA 1 cut(s) 59
MroI TCCGGA 1 cut(s) 976
MroXI GAANNNNTTC 1 cut(s) 98
MscI TGGCCA 1 cut(s) 59
MseI TTAA 2 cut(s) 1044, 1371
Msp20I TGGCCA 1 cut(s) 59
MspA1I CMGCKG 1 cut(s) 474
MvaI CCWGG 5 cut(s) 10, 257, 413, 785, 1826
MvnI CGCG 6 cut(s) 474, 570, 641, 1076, 1169, 1489
MwoI GCNNNNNNNGC 9 cut(s) 129, 146, 325, 1033, 1036, 1183, 1727, 1766, 1781
NarI GGCGCC 2 cut(s) 477, 1200
NciI CCSGG 8 cut(s) 107, 506, 721, 922, 938, 965, 1723, 1757
NlaIII CATG 9 cut(s) 64, 202, 247, 349, 373, 556, 1093, 1507, 1707
NmeAIII GCCGAG 5 cut(s) 616, 638, 652, 721, 1423
NmuCI GTSAC 2 cut(s) 994, 1271
NspI RCATGY 2 cut(s) 1093, 1707
NspV TTCGAA 1 cut(s) 876
PaeR7I CTCGAG 1 cut(s) 293
PciI ACATGT 1 cut(s) 1089
PciSI GCTCTTC 2 cut(s) 254, 721
PcsI WCGNNNNNNNCGW 4 cut(s) 291, 546, 567, 819
PdmI GAANNNNTTC 1 cut(s) 98
PfeI GAWTC 6 cut(s) 351, 384, 724, 973, 1066, 1743
PfoI TCCNGGA 3 cut(s) 8, 504, 719
PleI GAGTC 6 cut(s) 400, 536, 661, 788, 850, 1453
PluTI GGCGCC 2 cut(s) 480, 1203
PpsI GAGTC 6 cut(s) 400, 536, 661, 788, 850, 1453
Ppu21I YACGTR 3 cut(s) 55, 1539, 1796
PscI ACATGT 1 cut(s) 1089
Psp124BI GAGCTC 2 cut(s) 1086, 1106
Psp6I CCWGG 5 cut(s) 8, 255, 411, 783, 1824
PspFI CCCAGC 1 cut(s) 1640
PspGI CCWGG 5 cut(s) 8, 255, 411, 783, 1824
PspXI VCTCGAGB 1 cut(s) 293
PsrI GAACNNNNNNTAC 1 cut(s) 37
PstI CTGCAG 1 cut(s) 1774
PsuI RGATCY 1 cut(s) 96
RsaI GTAC 7 cut(s) 374, 443, 470, 830, 962, 1541, 1874
RsaNI GTAC 7 cut(s) 373, 442, 469, 829, 961, 1540, 1873
Rsr2I CGGWCCG 1 cut(s) 1188
RsrII CGGWCCG 1 cut(s) 1188
SacI GAGCTC 2 cut(s) 1086, 1106
SacII CCGCGG 1 cut(s) 475
SalI GTCGAC 1 cut(s) 539
SapI GCTCTTC 2 cut(s) 254, 721
SaqAI TTAA 2 cut(s) 1044, 1371
SchI GAGTC 6 cut(s) 401, 536, 661, 789, 850, 1454
SduI GDGCHC 4 cut(s) 426, 1086, 1106, 1140
SfaNI GCATC 3 cut(s) 132, 441, 1627
SfcI CTRYAG 1 cut(s) 1770
SfoI GGCGCC 2 cut(s) 478, 1201
Sfr274I CTCGAG 1 cut(s) 293
Sfr303I CCGCGG 1 cut(s) 475
SfuI TTCGAA 1 cut(s) 876
SgrBI CCGCGG 1 cut(s) 475
SinI GGWCC 7 cut(s) 109, 267, 409, 815, 851, 1154, 1188
SlaI CTCGAG 1 cut(s) 293
SmlI CTYRAG 1 cut(s) 293
SmoI CTYRAG 1 cut(s) 293
Sse9I AATT 7 cut(s) 745, 808, 871, 883, 980, 1111, 1845
SspDI GGCGCC 2 cut(s) 476, 1199
SspI AATATT 1 cut(s) 897
SspMI CTAG 2 cut(s) 1545, 1646
SstI GAGCTC 2 cut(s) 1086, 1106
StyI CCWWGG 2 cut(s) 1203, 1866
TaaI ACNGT 6 cut(s) 651, 910, 949, 1055, 1632, 1968
TaiI ACGT 8 cut(s) 57, 552, 621, 1146, 1313, 1541, 1593, 1798
TaqI TCGA 7 cut(s) 294, 465, 540, 561, 579, 876, 1619
TaqII GACCGA 1 cut(s) 1544
TasI AATT 7 cut(s) 745, 808, 871, 883, 980, 1111, 1845
TatI WGTACW 2 cut(s) 828, 1872
TauI GCSGC 8 cut(s) 221, 477, 518, 570, 1030, 1033, 1169, 1455
TfiI GAWTC 6 cut(s) 351, 384, 724, 973, 1066, 1743
Tru1I TTAA 2 cut(s) 1044, 1371
Tru9I TTAA 2 cut(s) 1044, 1371
TscAI CASTG 5 cut(s) 434, 1060, 1399, 1756, 1906
TseFI GTSAC 2 cut(s) 994, 1271
TseI GCWGC 7 cut(s) 399, 484, 1033, 1036, 1769, 1772, 1829
Tsp45I GTSAC 2 cut(s) 994, 1271
TspDTI ATGAA 4 cut(s) 163, 202, 215, 1871
TspGWI ACGGA 4 cut(s) 70, 300, 621, 760
TspRI CASTG 5 cut(s) 434, 1060, 1399, 1756, 1906
VpaK11BI GGWCC 7 cut(s) 109, 267, 409, 815, 851, 1154, 1188
XagI CCTNNNNNAGG 1 cut(s) 23
XapI RAATTY 2 cut(s) 883, 980
XceI RCATGY 2 cut(s) 1093, 1707
XcmI CCANNNNNNNNNTGG 1 cut(s) 1238
XhoI CTCGAG 1 cut(s) 293
XmiI GTMKAC 1 cut(s) 540
XmnI GAANNNNTTC 1 cut(s) 98
XspI CTAG 2 cut(s) 1545, 1646
ZraI GACGTC 1 cut(s) 550
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.