Rw5G003890

(2R)-phospho-3-sulfolactate synthase (ComA)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0007662
Physical Location & Seq
Forward (+)
3880160 .. 3882368
2209 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G003890.1

Sequence Viewer

Length: 831 bp
ATGTCGGCGTACCGATGGAAGTCCTTTCACGAGAACGAGGACCGGCCGGAGAAGCCTCGCCGGTACGGCGTCACAGAAATGAGAGGCCCCAATCACACTCTCTTGACCCAAAACGTCCTTCAGGTAATCGAATCGAACCGATATCCTCGCTCGCATAGCTTGATGCCCAAGTCGTTTGTTAAAGAGGTGATTGATGTGGCTCACCAACATGACGTGTATGTAAGCACTGGTGATTGGGCTGAGAATTTACTTCGAAAAGGCCCTTCGGCTTTTAAAGAGTATGTGGAGGAGTGTAAGAGCTTGGGATTTGACACAATTGAGCTGAATGTGGGATCTCTCGGAGTGCCTGAGGAAACGCTTCTGAGACTTGTGCGCTTGATTAAGAGCGGTGGCTTGAAGGTGAAGCCTCTGTTTGAAGTCAAGGTTAACGAGTCTGACATTCCTATTGGTGATAGAGCATTTGGAGCCTATGTCGTGCCGAGACCAAGATCATCTGAATTTGTAGAAGATGTTGATCTGTTGATTAGAAGGGCTGAGAGATGCTTAGAAGCTGGGGCTGACATGATAATGATTGAGGCTGATGATGTCTGTAAACAGGCTGATTCAATGCGGCCAGACATAATTGCAAAGGTCATTGGGCGCCTTGGTGTCGAGAAGACCATGTTTGAAGCATCAAATCCAAGAACCTCGGAGTGGTTCATCCAGCATTATGGTCCAAGGGTCAATCTGTTTGTGGACCACTCTCATGTGATGGACCTGGAGTGCCTCCGGGGACGCAACTTAGGTAGAAACCATGCATCTGTGTTGGGCTCCTCGTATTTTCTGTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ComA

Protein Analysis

276

Amino Acids

31.43

Weight (kDa)

6.33

Isoelectric Point (pI)

39.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ComA PF02679 14 - 260 2.5e-61 (2R)-phospho-3-sulfolactate synthase (ComA)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 829
AccB1I GGYRCC 1 cut(s) 639
AccBSI CCGCTC 1 cut(s) 387
AciI CCGC 2 cut(s) 387, 610
AclWI GGATC 1 cut(s) 340
AcoI YGGCCR 2 cut(s) 44, 611
AcsI RAATTY 2 cut(s) 244, 497
AcuI CTGAAG 1 cut(s) 104
AcyI GRCGYC 2 cut(s) 69, 640
AfaI GTAC 2 cut(s) 11, 65
AfiI CCNNNNNNNGG 1 cut(s) 693
AflIII ACRYGT 1 cut(s) 213
AgsI TTSAA 4 cut(s) 397, 416, 606, 668
AjiI CACGTC 1 cut(s) 214
AjnI CCWGG 1 cut(s) 756
AjuI GAANNNNNNNTTGG 2 cut(s) 102, 134
AluBI AGCT 4 cut(s) 159, 300, 322, 551
AluI AGCT 4 cut(s) 159, 300, 322, 551
Alw26I GTCTC 2 cut(s) 358, 475
AlwI GGATC 1 cut(s) 340
AoxI GGCC 4 cut(s) 44, 85, 259, 611
ApoI RAATTY 2 cut(s) 244, 497
Asp700I GAANNNNTTC 1 cut(s) 357
AspLEI GCGC 2 cut(s) 375, 642
AspS9I GGNCC 6 cut(s) 40, 86, 260, 713, 736, 754
AsuC2I CCSGG 1 cut(s) 770
AsuHPI GGTGA 5 cut(s) 194, 199, 242, 412, 461
AsuII TTCGAA 1 cut(s) 253
AvaII GGWCC 4 cut(s) 40, 713, 736, 754
AxyI CCTNAGG 1 cut(s) 348
BanI GGYRCC 1 cut(s) 639
BanII GRGCYC 1 cut(s) 812
BauI CACGAG 1 cut(s) 29
BbsI GAAGAC 1 cut(s) 662
BccI CCATC 2 cut(s) 9, 745
BceAI ACGGC 1 cut(s) 82
BciT130I CCWGG 1 cut(s) 758
BcnI CCSGG 1 cut(s) 770
BcoDI GTCTC 2 cut(s) 358, 475
BfoI RGCGCY 1 cut(s) 643
BglI GCCNNNNNGGC 1 cut(s) 66
BisI GCNGC 1 cut(s) 611
BlsI GCNGC 1 cut(s) 612
Bme1390I CCNGG 2 cut(s) 758, 770
Bme18I GGWCC 4 cut(s) 40, 713, 736, 754
BmgBI CACGTC 1 cut(s) 214
BmgT120I GGNCC 6 cut(s) 40, 86, 260, 713, 736, 754
BmiI GGNNCC 4 cut(s) 88, 466, 641, 811
BmrFI CCNGG 2 cut(s) 758, 770
BmsI GCATC 4 cut(s) 153, 530, 680, 806
BpiI GAAGAC 1 cut(s) 662
BpmI CTGGAG 1 cut(s) 779
Bpu14I TTCGAA 1 cut(s) 253
BpuMI CCSGG 1 cut(s) 770
BsaBI GATNNNNATC 1 cut(s) 513
BsaHI GRCGYC 2 cut(s) 69, 640
BsaI GGTCTC 1 cut(s) 475
BsaJI CCNNGG 4 cut(s) 643, 687, 716, 769
BsaXI ACNNNNNCTCC 2 cut(s) 456, 486
Bsc4I CCNNNNNNNGG 1 cut(s) 693
Bse118I RCCGGY 2 cut(s) 42, 60
Bse1I ACTGG 1 cut(s) 232
Bse21I CCTNAGG 1 cut(s) 348
Bse8I GATNNNNATC 1 cut(s) 513
BseBI CCWGG 1 cut(s) 758
BseDI CCNNGG 4 cut(s) 643, 687, 716, 769
BseGI GGATG 1 cut(s) 699
BseJI GATNNNNATC 1 cut(s) 513
BseLI CCNNNNNNNGG 1 cut(s) 693
BseMII CTCAG 4 cut(s) 231, 339, 353, 525
BseNI ACTGG 1 cut(s) 232
BseRI GAGGAG 2 cut(s) 302, 802
BseX3I CGGCCG 1 cut(s) 44
BseYI CCCAGC 1 cut(s) 551
Bsh1285I CGRYCG 1 cut(s) 47
BshFI GGCC 4 cut(s) 46, 87, 261, 613
BshNI GGYRCC 1 cut(s) 639
BsiEI CGRYCG 1 cut(s) 47
BsiSI CCGG 4 cut(s) 43, 47, 61, 769
BslFI GGGAC 1 cut(s) 786
BslI CCNNNNNNNGG 1 cut(s) 693
BsmAI GTCTC 2 cut(s) 358, 475
BsmFI GGGAC 1 cut(s) 786
BsnI GGCC 4 cut(s) 46, 87, 261, 613
Bso31I GGTCTC 1 cut(s) 475
Bsp119I TTCGAA 1 cut(s) 253
Bsp1286I GDGCHC 1 cut(s) 812
Bsp143I GATC 3 cut(s) 332, 488, 514
BspACI CCGC 2 cut(s) 387, 610
BspANI GGCC 4 cut(s) 46, 87, 261, 613
BspCNI CTCAG 4 cut(s) 232, 340, 354, 526
BspLI GGNNCC 4 cut(s) 88, 466, 641, 811
BspPI GGATC 1 cut(s) 340
BspT104I TTCGAA 1 cut(s) 253
BspT107I GGYRCC 1 cut(s) 639
BspTNI GGTCTC 1 cut(s) 475
BsrBI CCGCTC 1 cut(s) 387
BsrFI RCCGGY 2 cut(s) 42, 60
BsrI ACTGG 1 cut(s) 232
BssAI RCCGGY 2 cut(s) 42, 60
BssECI CCNNGG 4 cut(s) 643, 687, 716, 769
BssMI GATC 3 cut(s) 332, 488, 514
BssNI GRCGYC 2 cut(s) 69, 640
BssSI CACGAG 1 cut(s) 29
BssT1I CCWWGG 2 cut(s) 643, 716
Bst2BI CACGAG 1 cut(s) 29
Bst2UI CCWGG 1 cut(s) 758
BstACI GRCGYC 2 cut(s) 69, 640
BstBI TTCGAA 1 cut(s) 253
BstC8I GCNNGC 1 cut(s) 152
BstDEI CTNAG 6 cut(s) 240, 348, 362, 534, 544, 781
BstF5I GGATG 1 cut(s) 699
BstH2I RGCGCY 1 cut(s) 643
BstHHI GCGC 2 cut(s) 375, 642
BstKTI GATC 3 cut(s) 335, 491, 517
BstMAI GTCTC 2 cut(s) 358, 475
BstMBI GATC 3 cut(s) 332, 488, 514
BstMCI CGRYCG 1 cut(s) 47
BstMWI GCNNNNNNNGC 4 cut(s) 52, 66, 156, 464
BstNI CCWGG 1 cut(s) 758
BstSCI CCNGG 2 cut(s) 756, 768
BstV2I GAAGAC 1 cut(s) 662
BstX2I RGATCY 1 cut(s) 332
BstXI CCANNNNNNTGG 1 cut(s) 710
BstYI RGATCY 1 cut(s) 332
BstZI CGGCCG 1 cut(s) 44
Bsu36I CCTNAGG 1 cut(s) 348
BsuRI GGCC 4 cut(s) 46, 87, 261, 613
BtrI CACGTC 1 cut(s) 214
BtsCI GGATG 1 cut(s) 699
BtsIMutI CAGTG 1 cut(s) 225
Cac8I GCNNGC 1 cut(s) 152
CfoI GCGC 2 cut(s) 375, 642
Cfr10I RCCGGY 2 cut(s) 42, 60
Cfr13I GGNCC 6 cut(s) 40, 86, 260, 713, 736, 754
CseI GACGC 2 cut(s) 58, 783
Csp6I GTAC 2 cut(s) 10, 64
CviAII CATG 5 cut(s) 209, 562, 661, 746, 794
CviQI GTAC 2 cut(s) 10, 64
DdeI CTNAG 6 cut(s) 240, 348, 362, 534, 544, 781
DinI GGCGCC 1 cut(s) 641
DpnI GATC 3 cut(s) 334, 490, 516
DpnII GATC 3 cut(s) 332, 488, 514
DraI TTTAAA 1 cut(s) 274
EaeI YGGCCR 2 cut(s) 44, 611
EagI CGGCCG 1 cut(s) 44
EclXI CGGCCG 1 cut(s) 44
Eco130I CCWWGG 2 cut(s) 643, 716
Eco24I GRGCYC 1 cut(s) 812
Eco31I GGTCTC 1 cut(s) 475
Eco32I GATATC 1 cut(s) 143
Eco47I GGWCC 4 cut(s) 40, 713, 736, 754
Eco52I CGGCCG 1 cut(s) 44
Eco57I CTGAAG 1 cut(s) 104
Eco81I CCTNAGG 1 cut(s) 348
EcoO109I RGGNCCY 2 cut(s) 86, 260
EcoRII CCWGG 1 cut(s) 756
EcoRV GATATC 1 cut(s) 143
EcoT14I CCWWGG 2 cut(s) 643, 716
EcoT22I ATGCAT 1 cut(s) 799
EcoT38I GRGCYC 1 cut(s) 812
EgeI GGCGCC 1 cut(s) 641
EheI GGCGCC 1 cut(s) 641
ErhI CCWWGG 2 cut(s) 643, 716
FaeI CATG 5 cut(s) 212, 565, 664, 749, 797
FaqI GGGAC 1 cut(s) 786
FatI CATG 5 cut(s) 208, 561, 660, 745, 793
Fnu4HI GCNGC 1 cut(s) 611
FokI GGATG 1 cut(s) 686
FriOI GRGCYC 1 cut(s) 812
Fsp4HI GCNGC 1 cut(s) 611
GlaI GCGC 2 cut(s) 374, 641
GluI GCNGC 1 cut(s) 611
GsaI CCCAGC 1 cut(s) 555
GsuI CTGGAG 1 cut(s) 779
HaeII RGCGCY 1 cut(s) 643
HaeIII GGCC 4 cut(s) 46, 87, 261, 613
HapII CCGG 4 cut(s) 43, 47, 61, 769
HgaI GACGC 2 cut(s) 58, 783
HhaI GCGC 2 cut(s) 375, 642
Hin1I GRCGYC 2 cut(s) 69, 640
Hin1II CATG 5 cut(s) 212, 565, 664, 749, 797
Hin6I GCGC 2 cut(s) 373, 640
HinP1I GCGC 2 cut(s) 373, 640
HincII GTYRAC 1 cut(s) 427
HindII GTYRAC 1 cut(s) 427
HinfI GANTC 3 cut(s) 131, 431, 602
HpaI GTTAAC 1 cut(s) 427
HpaII CCGG 4 cut(s) 43, 47, 61, 769
HphI GGTGA 5 cut(s) 194, 199, 242, 412, 461
Hpy166II GTNNAC 3 cut(s) 427, 593, 736
Hpy188I TCNGA 5 cut(s) 341, 363, 436, 496, 691
Hpy188III TCNNGA 3 cut(s) 29, 103, 652
Hpy8I GTNNAC 3 cut(s) 427, 593, 736
HpyAV CCTTC 4 cut(s) 128, 273, 391, 522
HpyCH4IV ACGT 2 cut(s) 114, 213
HpyCH4V TGCA 2 cut(s) 626, 797
HpyF10VI GCNNNNNNNGC 4 cut(s) 52, 66, 156, 464
HpyF3I CTNAG 6 cut(s) 240, 348, 362, 534, 544, 781
HpySE526I ACGT 2 cut(s) 114, 213
Hsp92I GRCGYC 2 cut(s) 69, 640
Hsp92II CATG 5 cut(s) 212, 565, 664, 749, 797
HspAI GCGC 2 cut(s) 373, 640
KasI GGCGCC 1 cut(s) 639
KspAI GTTAAC 1 cut(s) 427
Kzo9I GATC 3 cut(s) 332, 488, 514
LmnI GCTCC 2 cut(s) 464, 815
LweI GCATC 4 cut(s) 153, 530, 680, 806
MaeII ACGT 2 cut(s) 114, 213
MaeIII GTNAC 1 cut(s) 70
MalI GATC 3 cut(s) 334, 490, 516
MbiI CCGCTC 1 cut(s) 387
MboI GATC 3 cut(s) 332, 488, 514
MboII GAAGA 2 cut(s) 518, 667
MfeI CAATTG 1 cut(s) 315
MflI RGATCY 1 cut(s) 332
MhlI GDGCHC 1 cut(s) 812
MluCI AATT 4 cut(s) 244, 315, 497, 621
Mly113I GGCGCC 1 cut(s) 640
MlyI GAGTC 1 cut(s) 440
Mph1103I ATGCAT 1 cut(s) 799
MroXI GAANNNNTTC 1 cut(s) 357
MseI TTAA 4 cut(s) 180, 273, 381, 426
MslI CAYNNNNRTG 4 cut(s) 77, 207, 566, 744
MspI CCGG 4 cut(s) 43, 47, 61, 769
MspR9I CCNGG 2 cut(s) 758, 770
MunI CAATTG 1 cut(s) 315
MvaI CCWGG 1 cut(s) 758
MwoI GCNNNNNNNGC 4 cut(s) 52, 66, 156, 464
NarI GGCGCC 1 cut(s) 640
NciI CCSGG 1 cut(s) 770
NdeII GATC 3 cut(s) 332, 488, 514
NlaIII CATG 5 cut(s) 212, 565, 664, 749, 797
NlaIV GGNNCC 4 cut(s) 88, 466, 641, 811
NmeAIII GCCGAG 1 cut(s) 504
NmuCI GTSAC 1 cut(s) 70
NsiI ATGCAT 1 cut(s) 799
NspV TTCGAA 1 cut(s) 253
PdmI GAANNNNTTC 1 cut(s) 357
PfeI GAWTC 2 cut(s) 131, 602
PkrI GCNGC 1 cut(s) 612
PleI GAGTC 1 cut(s) 439
PluTI GGCGCC 1 cut(s) 643
PpsI GAGTC 1 cut(s) 439
PsiI TTATAA 1 cut(s) 829
Psp6I CCWGG 1 cut(s) 756
PspFI CCCAGC 1 cut(s) 551
PspGI CCWGG 1 cut(s) 756
PspN4I GGNNCC 4 cut(s) 88, 466, 641, 811
PspPI GGNCC 6 cut(s) 40, 86, 260, 713, 736, 754
PsuI RGATCY 1 cut(s) 332
RsaI GTAC 2 cut(s) 11, 65
RsaNI GTAC 2 cut(s) 10, 64
RseI CAYNNNNRTG 4 cut(s) 77, 207, 566, 744
SaqAI TTAA 4 cut(s) 180, 273, 381, 426
SatI GCNGC 1 cut(s) 611
Sau3AI GATC 3 cut(s) 332, 488, 514
Sau96I GGNCC 6 cut(s) 40, 86, 260, 713, 736, 754
SchI GAGTC 1 cut(s) 440
ScrFI CCNGG 2 cut(s) 758, 770
SduI GDGCHC 1 cut(s) 812
SfaNI GCATC 4 cut(s) 153, 530, 680, 806
SfoI GGCGCC 1 cut(s) 641
SfuI TTCGAA 1 cut(s) 253
SinI GGWCC 4 cut(s) 40, 713, 736, 754
SmiMI CAYNNNNRTG 4 cut(s) 77, 207, 566, 744
Sse9I AATT 4 cut(s) 244, 315, 497, 621
SsiI CCGC 2 cut(s) 387, 610
SspDI GGCGCC 1 cut(s) 639
StyD4I CCNGG 2 cut(s) 756, 768
StyI CCWWGG 2 cut(s) 643, 716
TaiI ACGT 2 cut(s) 117, 216
TaqI TCGA 4 cut(s) 129, 134, 253, 651
TasI AATT 4 cut(s) 244, 315, 497, 621
TauI GCSGC 1 cut(s) 613
TfiI GAWTC 2 cut(s) 131, 602
Tru1I TTAA 4 cut(s) 180, 273, 381, 426
Tru9I TTAA 4 cut(s) 180, 273, 381, 426
TscAI CASTG 1 cut(s) 232
TseFI GTSAC 1 cut(s) 70
Tsp45I GTSAC 1 cut(s) 70
TspDTI ATGAA 1 cut(s) 688
TspRI CASTG 1 cut(s) 232
VpaK11BI GGWCC 4 cut(s) 40, 713, 736, 754
XapI RAATTY 2 cut(s) 244, 497
XmnI GAANNNNTTC 1 cut(s) 357
Zsp2I ATGCAT 1 cut(s) 799
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.