AT4G21320

(2R)-phospho-3-sulfolactate synthase (ComA)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0007662
Physical Location & Seq
Forward (+)
11340362 .. 11342075
1714 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G21320.1

Sequence Viewer

Length: 861 bp
ATGGCGGCTTACTACAGATGGAAGAGTTTCGAGGAGAACGAAGATCGTCCAGAGAAGCCTCGCCGTTACGGTGTTACGGAGATGCGAGGTCCTCATTACTCCGTTCTCAGCCAGAACCTTCTTCAGGAAATTTTTGAATCGATGGGACAGTTTGTTGATGGGTTAAAGTTCTCTGGAGGATCCAACAGTTTGATTCCAAAATCATTCATCAAACAAGCTATTGAAATGGCTCATGAACATGGTGTTTATGTTAGCACTGGTGATTGGGCTGAACATATGCTTCGTAGTGGTCCTTCAGCTTTCAAAGACTATGTGGAGGAGTGTAAGCAGTTGGGGTTTGACACTATTGAGCTGAATGCAAATTTGCTTGAAGTTCCTGAGGAAACTCTTCTACGATATGTACGTTTGATTAAGAACGGTGGTCTCAGAGCCAAACCAATGTTTGCTGTGAAATTCAACAAGTCTGATATTCCTGGTAGGAATAGAGCATTTGGTTCCTATGTTGTTCCAGAACCTCGGTCAAGCGAGTTTGTGGAGGACATAGATCTCTTGATCAGAAAGGCTGAACGGTGTTTAGAAGCCGGGGCAGACACTATTATGATAGATGCGGATGATGTCTGCAAATACGCGGATTCCCTTCGAGCAGACATAATTGCTAAAGTCATAGGACGTTTGGGAATAGAGAAGACTATGTTTGAAGCATCTGATGCGAAGTTGGTTGAGTGGTTCATCAAACGTTATGGTCCAAATGTGAATCTCTATGTGGATCATTCTCAGATTATGGATCTCGAGTGCCTCCGTGGTCGTCATCTGGGTAAAGATCACCAATCTGTTCTCAGTTCCTCCTACTTCTTGTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0008150 GO:0009266 GO:0009408 GO:0009628 GO:0010286 GO:0010468 GO:0010608 GO:0019222 GO:0050789 GO:0050896 GO:0060255 GO:0065007
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ComA

Protein Analysis

286

Amino Acids

32.64

Weight (kDa)

5.74

Isoelectric Point (pI)

44.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ComA PF02679 15 - 270 1.3e-73 (2R)-phospho-3-sulfolactate synthase (ComA)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 629
AciI CCGC 3 cut(s) 5, 608, 629
AclI AACGTT 1 cut(s) 736
AclWI GGATC 4 cut(s) 174, 187, 774, 792
AcsI RAATTY 3 cut(s) 129, 361, 452
AcuI CTGAAG 2 cut(s) 107, 279
AfaI GTAC 1 cut(s) 402
AfiI CCNNNNNNNGG 1 cut(s) 124
AgsI TTSAA 6 cut(s) 137, 224, 304, 371, 457, 698
AjnI CCWGG 1 cut(s) 472
AluBI AGCT 3 cut(s) 218, 299, 352
AluI AGCT 3 cut(s) 218, 299, 352
Alw26I GTCTC 1 cut(s) 428
AlwI GGATC 4 cut(s) 174, 187, 774, 792
Ama87I CYCGRG 1 cut(s) 788
ApoI RAATTY 3 cut(s) 129, 361, 452
Asp700I GAANNNNTTC 2 cut(s) 26, 387
AspS9I GGNCC 3 cut(s) 89, 290, 743
AsuC2I CCSGG 1 cut(s) 583
AsuHPI GGTGA 2 cut(s) 272, 815
AvaI CYCGRG 1 cut(s) 788
AvaII GGWCC 3 cut(s) 89, 290, 743
AxyI CCTNAGG 1 cut(s) 378
BamHI GGATCC 1 cut(s) 179
BarI GAAGNNNNNNTAC 2 cut(s) 277, 309
BbsI GAAGAC 1 cut(s) 692
BccI CCATC 3 cut(s) 12, 136, 152
BceAI ACGGC 1 cut(s) 48
BciT130I CCWGG 1 cut(s) 474
BclI TGATCA 1 cut(s) 552
BcnI CCSGG 1 cut(s) 583
BcoDI GTCTC 1 cut(s) 428
BfmI CTRYAG 1 cut(s) 13
BglII AGATCT 1 cut(s) 544
BisI GCNGC 1 cut(s) 6
BlsI GCNGC 1 cut(s) 7
Bme1390I CCNGG 2 cut(s) 474, 583
Bme18I GGWCC 3 cut(s) 89, 290, 743
BmeT110I CYCGRG 1 cut(s) 788
BmgT120I GGNCC 3 cut(s) 89, 290, 743
BmiI GGNNCC 2 cut(s) 181, 496
BmrFI CCNGG 2 cut(s) 474, 583
BmsI GCATC 4 cut(s) 72, 595, 697, 710
BpiI GAAGAC 1 cut(s) 692
BpmI CTGGAG 1 cut(s) 195
BpuMI CCSGG 1 cut(s) 583
Bsa29I ATCGAT 1 cut(s) 140
BsaI GGTCTC 1 cut(s) 428
BsaJI CCNNGG 3 cut(s) 515, 582, 799
Bsc4I CCNNNNNNNGG 1 cut(s) 124
Bse1I ACTGG 1 cut(s) 262
Bse21I CCTNAGG 1 cut(s) 378
BseBI CCWGG 1 cut(s) 474
BseCI ATCGAT 1 cut(s) 140
BseDI CCNNGG 3 cut(s) 515, 582, 799
BseGI GGATG 1 cut(s) 616
BseLI CCNNNNNNNGG 1 cut(s) 124
BseMII CTCAG 5 cut(s) 121, 369, 439, 788, 850
BseNI ACTGG 1 cut(s) 262
BseRI GAGGAG 2 cut(s) 47, 332
Bsh1236I CGCG 1 cut(s) 629
BshVI ATCGAT 1 cut(s) 140
BsiHKCI CYCGRG 1 cut(s) 788
BsiSI CCGG 1 cut(s) 582
BslFI GGGAC 1 cut(s) 159
BslI CCNNNNNNNGG 1 cut(s) 124
BsmAI GTCTC 1 cut(s) 428
BsmFI GGGAC 1 cut(s) 159
BsmI GAATGC 1 cut(s) 361
Bso31I GGTCTC 1 cut(s) 428
BsoBI CYCGRG 1 cut(s) 788
Bsp143I GATC 7 cut(s) 43, 179, 544, 552, 766, 784, 820
BspACI CCGC 3 cut(s) 5, 608, 629
BspCNI CTCAG 5 cut(s) 120, 370, 438, 787, 849
BspDI ATCGAT 1 cut(s) 140
BspFNI CGCG 1 cut(s) 629
BspHI TCATGA 1 cut(s) 232
BspLI GGNNCC 2 cut(s) 181, 496
BspPI GGATC 4 cut(s) 174, 187, 774, 792
BspTNI GGTCTC 1 cut(s) 428
BsrI ACTGG 1 cut(s) 262
BssECI CCNNGG 3 cut(s) 515, 582, 799
BssMI GATC 7 cut(s) 43, 179, 544, 552, 766, 784, 820
Bst2UI CCWGG 1 cut(s) 474
Bst4CI ACNGT 5 cut(s) 71, 150, 188, 419, 570
Bst6I CTCTTC 2 cut(s) 17, 393
BstAPI GCANNNNNTGC 1 cut(s) 707
BstDEI CTNAG 5 cut(s) 107, 378, 425, 774, 836
BstDSI CCRYGG 1 cut(s) 799
BstENI CCTNNNNNAGG 1 cut(s) 122
BstF5I GGATG 1 cut(s) 616
BstFNI CGCG 1 cut(s) 629
BstKTI GATC 7 cut(s) 46, 182, 547, 555, 769, 787, 823
BstMAI GTCTC 1 cut(s) 428
BstMBI GATC 7 cut(s) 43, 179, 544, 552, 766, 784, 820
BstMWI GCNNNNNNNGC 1 cut(s) 707
BstNI CCWGG 1 cut(s) 474
BstSCI CCNGG 2 cut(s) 472, 581
BstSFI CTRYAG 1 cut(s) 13
BstUI CGCG 1 cut(s) 629
BstV2I GAAGAC 1 cut(s) 692
BstX2I RGATCY 3 cut(s) 179, 544, 784
BstYI RGATCY 3 cut(s) 179, 544, 784
Bsu15I ATCGAT 1 cut(s) 140
Bsu36I CCTNAGG 1 cut(s) 378
BsuTUI ATCGAT 1 cut(s) 140
BtgI CCRYGG 1 cut(s) 799
BtsCI GGATG 1 cut(s) 616
BtsIMutI CAGTG 1 cut(s) 255
CciI TCATGA 1 cut(s) 232
Cfr13I GGNCC 3 cut(s) 89, 290, 743
ClaI ATCGAT 1 cut(s) 140
Csp6I GTAC 1 cut(s) 401
CviAII CATG 2 cut(s) 233, 239
CviQI GTAC 1 cut(s) 401
DdeI CTNAG 5 cut(s) 107, 378, 425, 774, 836
DpnI GATC 7 cut(s) 45, 181, 546, 554, 768, 786, 822
DpnII GATC 7 cut(s) 43, 179, 544, 552, 766, 784, 820
Eam1104I CTCTTC 2 cut(s) 17, 393
EarI CTCTTC 2 cut(s) 17, 393
Eco31I GGTCTC 1 cut(s) 428
Eco47I GGWCC 3 cut(s) 89, 290, 743
Eco57I CTGAAG 2 cut(s) 107, 279
Eco81I CCTNAGG 1 cut(s) 378
Eco88I CYCGRG 1 cut(s) 788
EcoNI CCTNNNNNAGG 1 cut(s) 122
EcoO109I RGGNCCY 1 cut(s) 89
EcoRII CCWGG 1 cut(s) 472
FaeI CATG 2 cut(s) 236, 242
FaqI GGGAC 1 cut(s) 159
FatI CATG 2 cut(s) 232, 238
FauNDI CATATG 1 cut(s) 276
FbaI TGATCA 1 cut(s) 552
Fnu4HI GCNGC 1 cut(s) 6
FokI GGATG 1 cut(s) 623
Fsp4HI GCNGC 1 cut(s) 6
GluI GCNGC 1 cut(s) 6
GsuI CTGGAG 1 cut(s) 195
HapII CCGG 1 cut(s) 582
Hin1II CATG 2 cut(s) 236, 242
HinfI GANTC 4 cut(s) 137, 193, 632, 754
HpaII CCGG 1 cut(s) 582
HphI GGTGA 2 cut(s) 272, 815
Hpy188I TCNGA 5 cut(s) 428, 466, 557, 706, 777
Hpy188III TCNNGA 8 cut(s) 50, 125, 174, 233, 377, 509, 550, 788
HpyAV CCTTC 3 cut(s) 128, 303, 647
HpyCH4III ACNGT 5 cut(s) 71, 150, 188, 419, 570
HpyCH4IV ACGT 3 cut(s) 403, 670, 736
HpyCH4V TGCA 2 cut(s) 359, 621
HpyF10VI GCNNNNNNNGC 1 cut(s) 707
HpyF3I CTNAG 5 cut(s) 107, 378, 425, 774, 836
HpySE526I ACGT 3 cut(s) 403, 670, 736
Hsp92II CATG 2 cut(s) 236, 242
Ksp22I TGATCA 1 cut(s) 552
Kzo9I GATC 7 cut(s) 43, 179, 544, 552, 766, 784, 820
LweI GCATC 4 cut(s) 72, 595, 697, 710
MaeII ACGT 3 cut(s) 403, 670, 736
MaeIII GTNAC 2 cut(s) 65, 73
MalI GATC 7 cut(s) 45, 181, 546, 554, 768, 786, 822
MboI GATC 7 cut(s) 43, 179, 544, 552, 766, 784, 820
MboII GAAGA 5 cut(s) 34, 53, 113, 380, 697
MflI RGATCY 3 cut(s) 179, 544, 784
MluCI AATT 4 cut(s) 129, 361, 452, 651
MmeI TCCRAC 1 cut(s) 207
MroXI GAANNNNTTC 2 cut(s) 26, 387
MseI TTAA 3 cut(s) 164, 411, 859
MslI CAYNNNNRTG 2 cut(s) 237, 596
MspI CCGG 1 cut(s) 582
MspR9I CCNGG 2 cut(s) 474, 583
Mva1269I GAATGC 1 cut(s) 361
MvaI CCWGG 1 cut(s) 474
MvnI CGCG 1 cut(s) 629
MwoI GCNNNNNNNGC 1 cut(s) 707
NciI CCSGG 1 cut(s) 583
NdeI CATATG 1 cut(s) 276
NdeII GATC 7 cut(s) 43, 179, 544, 552, 766, 784, 820
NlaIII CATG 2 cut(s) 236, 242
NlaIV GGNNCC 2 cut(s) 181, 496
PaeR7I CTCGAG 1 cut(s) 788
PagI TCATGA 1 cut(s) 232
PcsI WCGNNNNNNNCGW 2 cut(s) 36, 400
PctI GAATGC 1 cut(s) 361
PdmI GAANNNNTTC 2 cut(s) 26, 387
PfeI GAWTC 4 cut(s) 137, 193, 632, 754
PkrI GCNGC 1 cut(s) 7
PpuMI RGGWCCY 1 cut(s) 89
Psp1406I AACGTT 1 cut(s) 736
Psp5II RGGWCCY 1 cut(s) 89
Psp6I CCWGG 1 cut(s) 472
PspGI CCWGG 1 cut(s) 472
PspN4I GGNNCC 2 cut(s) 181, 496
PspPI GGNCC 3 cut(s) 89, 290, 743
PspPPI RGGWCCY 1 cut(s) 89
PsuI RGATCY 3 cut(s) 179, 544, 784
RsaI GTAC 1 cut(s) 402
RsaNI GTAC 1 cut(s) 401
RseI CAYNNNNRTG 2 cut(s) 237, 596
SaqAI TTAA 3 cut(s) 164, 411, 859
SatI GCNGC 1 cut(s) 6
Sau3AI GATC 7 cut(s) 43, 179, 544, 552, 766, 784, 820
Sau96I GGNCC 3 cut(s) 89, 290, 743
ScrFI CCNGG 2 cut(s) 474, 583
SetI ASST 9 cut(s) 91, 120, 220, 301, 354, 406, 517, 673, 739
SfaNI GCATC 4 cut(s) 72, 595, 697, 710
SfcI CTRYAG 1 cut(s) 13
Sfr274I CTCGAG 1 cut(s) 788
SinI GGWCC 3 cut(s) 89, 290, 743
SlaI CTCGAG 1 cut(s) 788
SmiMI CAYNNNNRTG 2 cut(s) 237, 596
SmlI CTYRAG 1 cut(s) 788
SmoI CTYRAG 1 cut(s) 788
Sse9I AATT 4 cut(s) 129, 361, 452, 651
SsiI CCGC 3 cut(s) 5, 608, 629
StyD4I CCNGG 2 cut(s) 472, 581
TaaI ACNGT 5 cut(s) 71, 150, 188, 419, 570
TaiI ACGT 3 cut(s) 406, 673, 739
TaqI TCGA 4 cut(s) 30, 140, 640, 789
TaqII GACCGA 1 cut(s) 507
TasI AATT 4 cut(s) 129, 361, 452, 651
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 4 cut(s) 137, 193, 632, 754
Tru1I TTAA 3 cut(s) 164, 411, 859
Tru9I TTAA 3 cut(s) 164, 411, 859
TscAI CASTG 1 cut(s) 262
TspDTI ATGAA 3 cut(s) 196, 249, 718
TspGWI ACGGA 3 cut(s) 91, 92, 788
TspRI CASTG 1 cut(s) 262
VpaK11BI GGWCC 3 cut(s) 89, 290, 743
XagI CCTNNNNNAGG 1 cut(s) 122
XapI RAATTY 3 cut(s) 129, 361, 452
XhoI CTCGAG 1 cut(s) 788
XmnI GAANNNNTTC 2 cut(s) 26, 387
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.