Rh5AG040500

(2R)-phospho-3-sulfolactate synthase (ComA)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0007662
Physical Location & Seq
Forward (+)
3034936 .. 3038258
3323 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG040500.1

Sequence Viewer

Length: 720 bp
ATGGGGGACATTGTGGATGGGCTCAAGTTTGCTGGAGGCTCGCATAGCTTGATGCCCAAGTCGTTTGTTAAAGAGGTGATTGATGTGGCTCACCAACATGACGTGTATGTAAGCACTGGTGATTGGGCTGAGAATTTACTTCGAAAAGGCCCTTCGGCTTTTAAAGAGTATGTGGAGGAGTGTAAGAGCTTGGGATTTGACACAATTGAGCTGAATGTGGGATCTCTCGGAGTGCCTGAGGAAACGCTTCTGAGACTTGTGCGCTTGATTAAGAGCGGTGGCTTGAAGGTGAAGCCTCTGTTTGAAGTCAAGGTTAACGAGTCTGACATTCCTATTGGTGATAGAGCATTTGGAGCCTATGTCGTGCCGAGACCAAGATCATCTGAATTTGTTGAAGATGTTGATCTGTTGATTAGAAGGGCTGAGAGATGCTTAGAAGCTGGGGCTGACATGATAATGATTGAGGCTGATGATGTCTGTAAACAGGCTGATTCAATGCGGCCAGACATAATTGCAAAGGTCATTGGGCGCCTTGGTGTCGAGAAGACCATGTTTGAAGCATCAAATCCAAGAACCTCGGAGTGGTTCATCCAGCATTATGGTCCAAGGGTCAATCTGTTTGTGGACCACTCTCATGTGATGGACCTGGAGTGTCTCCGGGGACGCAACTTAGGTAGAAACCATGCATCTGTGTTGGGCTCCTCGTATTTTCTGTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ComA

Protein Analysis

239

Amino Acids

26.62

Weight (kDa)

5.55

Isoelectric Point (pI)

31.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ComA PF02679 1 - 223 1.7e-64 (2R)-phospho-3-sulfolactate synthase (ComA)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 718
AccB1I GGYRCC 1 cut(s) 528
AccBSI CCGCTC 1 cut(s) 276
AciI CCGC 2 cut(s) 276, 499
AclWI GGATC 1 cut(s) 229
AcoI YGGCCR 1 cut(s) 500
AcsI RAATTY 2 cut(s) 133, 386
AcyI GRCGYC 1 cut(s) 529
AfiI CCNNNNNNNGG 1 cut(s) 582
AflIII ACRYGT 1 cut(s) 102
AgsI TTSAA 5 cut(s) 286, 305, 395, 495, 557
AjiI CACGTC 1 cut(s) 103
AjnI CCWGG 1 cut(s) 645
AluBI AGCT 4 cut(s) 48, 189, 211, 440
AluI AGCT 4 cut(s) 48, 189, 211, 440
Alw26I GTCTC 3 cut(s) 247, 364, 659
AlwI GGATC 1 cut(s) 229
AoxI GGCC 2 cut(s) 148, 500
ApoI RAATTY 2 cut(s) 133, 386
Asp700I GAANNNNTTC 1 cut(s) 246
AspLEI GCGC 2 cut(s) 264, 531
AspS9I GGNCC 4 cut(s) 149, 602, 625, 643
AsuC2I CCSGG 1 cut(s) 659
AsuHPI GGTGA 5 cut(s) 83, 88, 131, 301, 350
AsuII TTCGAA 1 cut(s) 142
AvaII GGWCC 3 cut(s) 602, 625, 643
AxyI CCTNAGG 1 cut(s) 237
BanI GGYRCC 1 cut(s) 528
BanII GRGCYC 2 cut(s) 24, 701
BbsI GAAGAC 1 cut(s) 551
BccI CCATC 2 cut(s) 11, 634
BciT130I CCWGG 1 cut(s) 647
BcnI CCSGG 1 cut(s) 659
BcoDI GTCTC 3 cut(s) 247, 364, 659
BfoI RGCGCY 1 cut(s) 532
BisI GCNGC 1 cut(s) 500
BlsI GCNGC 1 cut(s) 501
Bme1390I CCNGG 2 cut(s) 647, 659
Bme18I GGWCC 3 cut(s) 602, 625, 643
BmgBI CACGTC 1 cut(s) 103
BmgT120I GGNCC 4 cut(s) 149, 602, 625, 643
BmiI GGNNCC 3 cut(s) 355, 530, 700
BmrFI CCNGG 2 cut(s) 647, 659
BmsI GCATC 4 cut(s) 42, 419, 569, 695
BpiI GAAGAC 1 cut(s) 551
BpmI CTGGAG 2 cut(s) 54, 668
Bpu14I TTCGAA 1 cut(s) 142
BpuEI CTTGAG 1 cut(s) 8
BpuMI CCSGG 1 cut(s) 659
BsaBI GATNNNNATC 1 cut(s) 402
BsaHI GRCGYC 1 cut(s) 529
BsaI GGTCTC 1 cut(s) 364
BsaJI CCNNGG 4 cut(s) 532, 576, 605, 658
BsaXI ACNNNNNCTCC 2 cut(s) 345, 375
Bsc4I CCNNNNNNNGG 1 cut(s) 582
Bse1I ACTGG 1 cut(s) 121
Bse21I CCTNAGG 1 cut(s) 237
Bse8I GATNNNNATC 1 cut(s) 402
BseBI CCWGG 1 cut(s) 647
BseDI CCNNGG 4 cut(s) 532, 576, 605, 658
BseGI GGATG 2 cut(s) 22, 588
BseJI GATNNNNATC 1 cut(s) 402
BseLI CCNNNNNNNGG 1 cut(s) 582
BseMII CTCAG 4 cut(s) 120, 228, 242, 414
BseNI ACTGG 1 cut(s) 121
BseRI GAGGAG 2 cut(s) 191, 691
BseYI CCCAGC 1 cut(s) 440
BshFI GGCC 2 cut(s) 150, 502
BshNI GGYRCC 1 cut(s) 528
BsiSI CCGG 1 cut(s) 658
BslFI GGGAC 2 cut(s) 20, 675
BslI CCNNNNNNNGG 1 cut(s) 582
BsmAI GTCTC 3 cut(s) 247, 364, 659
BsmFI GGGAC 2 cut(s) 20, 675
BsnI GGCC 2 cut(s) 150, 502
Bso31I GGTCTC 1 cut(s) 364
Bsp119I TTCGAA 1 cut(s) 142
Bsp1286I GDGCHC 2 cut(s) 24, 701
Bsp143I GATC 3 cut(s) 221, 377, 403
BspACI CCGC 2 cut(s) 276, 499
BspANI GGCC 2 cut(s) 150, 502
BspCNI CTCAG 4 cut(s) 121, 229, 243, 415
BspLI GGNNCC 3 cut(s) 355, 530, 700
BspPI GGATC 1 cut(s) 229
BspT104I TTCGAA 1 cut(s) 142
BspT107I GGYRCC 1 cut(s) 528
BspTNI GGTCTC 1 cut(s) 364
BsrBI CCGCTC 1 cut(s) 276
BsrI ACTGG 1 cut(s) 121
BssECI CCNNGG 4 cut(s) 532, 576, 605, 658
BssMI GATC 3 cut(s) 221, 377, 403
BssNI GRCGYC 1 cut(s) 529
BssT1I CCWWGG 2 cut(s) 532, 605
Bst2UI CCWGG 1 cut(s) 647
BstACI GRCGYC 1 cut(s) 529
BstBI TTCGAA 1 cut(s) 142
BstC8I GCNNGC 1 cut(s) 41
BstDEI CTNAG 6 cut(s) 129, 237, 251, 423, 433, 670
BstF5I GGATG 2 cut(s) 22, 588
BstH2I RGCGCY 1 cut(s) 532
BstHHI GCGC 2 cut(s) 264, 531
BstKTI GATC 3 cut(s) 224, 380, 406
BstMAI GTCTC 3 cut(s) 247, 364, 659
BstMBI GATC 3 cut(s) 221, 377, 403
BstMWI GCNNNNNNNGC 2 cut(s) 45, 353
BstNI CCWGG 1 cut(s) 647
BstSCI CCNGG 2 cut(s) 645, 657
BstV2I GAAGAC 1 cut(s) 551
BstX2I RGATCY 1 cut(s) 221
BstXI CCANNNNNNTGG 1 cut(s) 599
BstYI RGATCY 1 cut(s) 221
Bsu36I CCTNAGG 1 cut(s) 237
BsuRI GGCC 2 cut(s) 150, 502
BtrI CACGTC 1 cut(s) 103
BtsCI GGATG 2 cut(s) 22, 588
BtsIMutI CAGTG 1 cut(s) 114
Cac8I GCNNGC 1 cut(s) 41
CfoI GCGC 2 cut(s) 264, 531
Cfr13I GGNCC 4 cut(s) 149, 602, 625, 643
CseI GACGC 1 cut(s) 672
CviAII CATG 5 cut(s) 98, 451, 550, 635, 683
DdeI CTNAG 6 cut(s) 129, 237, 251, 423, 433, 670
DinI GGCGCC 1 cut(s) 530
DpnI GATC 3 cut(s) 223, 379, 405
DpnII GATC 3 cut(s) 221, 377, 403
DraI TTTAAA 1 cut(s) 163
EaeI YGGCCR 1 cut(s) 500
Eco130I CCWWGG 2 cut(s) 532, 605
Eco24I GRGCYC 2 cut(s) 24, 701
Eco31I GGTCTC 1 cut(s) 364
Eco47I GGWCC 3 cut(s) 602, 625, 643
Eco81I CCTNAGG 1 cut(s) 237
EcoO109I RGGNCCY 1 cut(s) 149
EcoRII CCWGG 1 cut(s) 645
EcoT14I CCWWGG 2 cut(s) 532, 605
EcoT22I ATGCAT 1 cut(s) 688
EcoT38I GRGCYC 2 cut(s) 24, 701
EgeI GGCGCC 1 cut(s) 530
EheI GGCGCC 1 cut(s) 530
ErhI CCWWGG 2 cut(s) 532, 605
FaeI CATG 5 cut(s) 101, 454, 553, 638, 686
FaqI GGGAC 2 cut(s) 20, 675
FatI CATG 5 cut(s) 97, 450, 549, 634, 682
Fnu4HI GCNGC 1 cut(s) 500
FokI GGATG 2 cut(s) 29, 575
FriOI GRGCYC 2 cut(s) 24, 701
Fsp4HI GCNGC 1 cut(s) 500
GlaI GCGC 2 cut(s) 263, 530
GluI GCNGC 1 cut(s) 500
GsaI CCCAGC 1 cut(s) 444
GsuI CTGGAG 2 cut(s) 54, 668
HaeII RGCGCY 1 cut(s) 532
HaeIII GGCC 2 cut(s) 150, 502
HapII CCGG 1 cut(s) 658
HgaI GACGC 1 cut(s) 672
HhaI GCGC 2 cut(s) 264, 531
Hin1I GRCGYC 1 cut(s) 529
Hin1II CATG 5 cut(s) 101, 454, 553, 638, 686
Hin6I GCGC 2 cut(s) 262, 529
HinP1I GCGC 2 cut(s) 262, 529
HincII GTYRAC 1 cut(s) 316
HindII GTYRAC 1 cut(s) 316
HinfI GANTC 2 cut(s) 320, 491
HpaI GTTAAC 1 cut(s) 316
HpaII CCGG 1 cut(s) 658
HphI GGTGA 5 cut(s) 83, 88, 131, 301, 350
Hpy166II GTNNAC 3 cut(s) 316, 482, 625
Hpy188I TCNGA 5 cut(s) 230, 252, 325, 385, 580
Hpy188III TCNNGA 1 cut(s) 541
Hpy8I GTNNAC 3 cut(s) 316, 482, 625
HpyAV CCTTC 3 cut(s) 162, 280, 411
HpyCH4IV ACGT 1 cut(s) 102
HpyCH4V TGCA 2 cut(s) 515, 686
HpyF10VI GCNNNNNNNGC 2 cut(s) 45, 353
HpyF3I CTNAG 6 cut(s) 129, 237, 251, 423, 433, 670
HpySE526I ACGT 1 cut(s) 102
Hsp92I GRCGYC 1 cut(s) 529
Hsp92II CATG 5 cut(s) 101, 454, 553, 638, 686
HspAI GCGC 2 cut(s) 262, 529
KasI GGCGCC 1 cut(s) 528
KspAI GTTAAC 1 cut(s) 316
Kzo9I GATC 3 cut(s) 221, 377, 403
LmnI GCTCC 2 cut(s) 353, 704
LweI GCATC 4 cut(s) 42, 419, 569, 695
MaeII ACGT 1 cut(s) 102
MalI GATC 3 cut(s) 223, 379, 405
MbiI CCGCTC 1 cut(s) 276
MboI GATC 3 cut(s) 221, 377, 403
MboII GAAGA 2 cut(s) 407, 556
MfeI CAATTG 1 cut(s) 204
MflI RGATCY 1 cut(s) 221
MhlI GDGCHC 2 cut(s) 24, 701
MluCI AATT 4 cut(s) 133, 204, 386, 510
Mly113I GGCGCC 1 cut(s) 529
MlyI GAGTC 1 cut(s) 329
MnlI CCTC 8 cut(s) 29, 67, 169, 232, 306, 457, 586, 712
Mph1103I ATGCAT 1 cut(s) 688
MroXI GAANNNNTTC 1 cut(s) 246
MseI TTAA 4 cut(s) 69, 162, 270, 315
MslI CAYNNNNRTG 3 cut(s) 96, 455, 633
MspI CCGG 1 cut(s) 658
MspR9I CCNGG 2 cut(s) 647, 659
MunI CAATTG 1 cut(s) 204
MvaI CCWGG 1 cut(s) 647
MwoI GCNNNNNNNGC 2 cut(s) 45, 353
NarI GGCGCC 1 cut(s) 529
NciI CCSGG 1 cut(s) 659
NdeII GATC 3 cut(s) 221, 377, 403
NlaIII CATG 5 cut(s) 101, 454, 553, 638, 686
NlaIV GGNNCC 3 cut(s) 355, 530, 700
NmeAIII GCCGAG 1 cut(s) 393
NsiI ATGCAT 1 cut(s) 688
NspV TTCGAA 1 cut(s) 142
PdmI GAANNNNTTC 1 cut(s) 246
PfeI GAWTC 1 cut(s) 491
PkrI GCNGC 1 cut(s) 501
PleI GAGTC 1 cut(s) 328
PluTI GGCGCC 1 cut(s) 532
PpsI GAGTC 1 cut(s) 328
PsiI TTATAA 1 cut(s) 718
Psp6I CCWGG 1 cut(s) 645
PspFI CCCAGC 1 cut(s) 440
PspGI CCWGG 1 cut(s) 645
PspN4I GGNNCC 3 cut(s) 355, 530, 700
PspPI GGNCC 4 cut(s) 149, 602, 625, 643
PsuI RGATCY 1 cut(s) 221
RseI CAYNNNNRTG 3 cut(s) 96, 455, 633
SaqAI TTAA 4 cut(s) 69, 162, 270, 315
SatI GCNGC 1 cut(s) 500
Sau3AI GATC 3 cut(s) 221, 377, 403
Sau96I GGNCC 4 cut(s) 149, 602, 625, 643
SchI GAGTC 1 cut(s) 329
ScrFI CCNGG 2 cut(s) 647, 659
SduI GDGCHC 2 cut(s) 24, 701
SfaNI GCATC 4 cut(s) 42, 419, 569, 695
SfoI GGCGCC 1 cut(s) 530
SfuI TTCGAA 1 cut(s) 142
SinI GGWCC 3 cut(s) 602, 625, 643
SmiMI CAYNNNNRTG 3 cut(s) 96, 455, 633
SmlI CTYRAG 1 cut(s) 23
SmoI CTYRAG 1 cut(s) 23
Sse9I AATT 4 cut(s) 133, 204, 386, 510
SsiI CCGC 2 cut(s) 276, 499
SspDI GGCGCC 1 cut(s) 528
StyD4I CCNGG 2 cut(s) 645, 657
StyI CCWWGG 2 cut(s) 532, 605
TaiI ACGT 1 cut(s) 105
TaqI TCGA 2 cut(s) 142, 540
TasI AATT 4 cut(s) 133, 204, 386, 510
TauI GCSGC 1 cut(s) 502
TfiI GAWTC 1 cut(s) 491
Tru1I TTAA 4 cut(s) 69, 162, 270, 315
Tru9I TTAA 4 cut(s) 69, 162, 270, 315
TscAI CASTG 1 cut(s) 121
TspDTI ATGAA 1 cut(s) 577
TspRI CASTG 1 cut(s) 121
VpaK11BI GGWCC 3 cut(s) 602, 625, 643
XapI RAATTY 2 cut(s) 133, 386
XmnI GAANNNNTTC 1 cut(s) 246
Zsp2I ATGCAT 1 cut(s) 688
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.