RchiOBHm_Chr5g0004571

(2R)-phospho-3-sulfolactate synthase (ComA)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0007662
Physical Location & Seq
Forward (+)
2974907 .. 2975513
607 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ28580

Sequence Viewer

Length: 438 bp
ATGGAAGACGTTCCACGAGAACGAAGTTATGGGATTTTAGGGTTTAGGGTTTTGGATATTTTCGAGTCGATGGGCAACATTGTTTATGGGCTGAAGTTTGCTGGAGTGATTGATGTGGCTCACCAACATGACGTGTATGTAAGCACTGAGGATTGGGCTAAGAATTTGCTTCTAAAAGACCCTTCGGCTTTTAAGGAGCATGTGGAGGAGTGTAAGAGCTTGGGATTTGACACAATTGAGCTGAATGTGGGATCTCTCGGAGTGCCTGAGGAAACGCTTCTAAGACTTGTGCGCTTGATTAAGAGCGGTGGCTTGAAAGTGAAGCCTCTGTTTGAAGTCAAGGTTAACGAGTCTGAAATTCCTACTGGTGATAGAACGTTTAGAGCCTATGTCGTCCCGAGACCAAGATCATCTGGTATGCTATGCACTCTTTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ComA

Protein Analysis

145

Amino Acids

16.23

Weight (kDa)

5.64

Isoelectric Point (pI)

39.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ComA PF02679 36 - 120 1.8e-17 (2R)-phospho-3-sulfolactate synthase (ComA)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 306
AciI CCGC 1 cut(s) 306
AclI AACGTT 1 cut(s) 377
AclWI GGATC 1 cut(s) 259
AcsI RAATTY 2 cut(s) 163, 357
AcuI CTGAAG 1 cut(s) 113
AflIII ACRYGT 1 cut(s) 132
AgsI TTSAA 2 cut(s) 316, 335
AjiI CACGTC 1 cut(s) 133
AluBI AGCT 2 cut(s) 219, 241
AluI AGCT 2 cut(s) 219, 241
Alw26I GTCTC 1 cut(s) 394
AlwI GGATC 1 cut(s) 259
Ama87I CYCGRG 1 cut(s) 397
ApoI RAATTY 2 cut(s) 163, 357
Asp700I GAANNNNTTC 2 cut(s) 9, 276
AspLEI GCGC 1 cut(s) 294
AsuHPI GGTGA 2 cut(s) 113, 380
AvaI CYCGRG 1 cut(s) 397
AxyI CCTNAGG 1 cut(s) 267
BauI CACGAG 1 cut(s) 15
BbsI GAAGAC 1 cut(s) 12
BccI CCATC 1 cut(s) 64
BcoDI GTCTC 1 cut(s) 394
BmeT110I CYCGRG 1 cut(s) 397
BmgBI CACGTC 1 cut(s) 133
BpiI GAAGAC 1 cut(s) 12
BpmI CTGGAG 1 cut(s) 123
BsaI GGTCTC 1 cut(s) 394
Bse1I ACTGG 1 cut(s) 370
Bse21I CCTNAGG 1 cut(s) 267
BseMII CTCAG 2 cut(s) 138, 258
BseNI ACTGG 1 cut(s) 370
BseRI GAGGAG 1 cut(s) 221
BsiHKCI CYCGRG 1 cut(s) 397
BslFI GGGAC 1 cut(s) 380
BsmAI GTCTC 1 cut(s) 394
BsmFI GGGAC 1 cut(s) 380
Bso31I GGTCTC 1 cut(s) 394
BsoBI CYCGRG 1 cut(s) 397
Bsp143I GATC 2 cut(s) 251, 407
BspACI CCGC 1 cut(s) 306
BspCNI CTCAG 2 cut(s) 139, 259
BspPI GGATC 1 cut(s) 259
BspTNI GGTCTC 1 cut(s) 394
BsrBI CCGCTC 1 cut(s) 306
BsrI ACTGG 1 cut(s) 370
BssMI GATC 2 cut(s) 251, 407
BssSI CACGAG 1 cut(s) 15
Bst2BI CACGAG 1 cut(s) 15
BstDEI CTNAG 4 cut(s) 147, 159, 267, 281
BstHHI GCGC 1 cut(s) 294
BstKTI GATC 2 cut(s) 254, 410
BstMAI GTCTC 1 cut(s) 394
BstMBI GATC 2 cut(s) 251, 407
BstNSI RCATGY 1 cut(s) 203
BstV2I GAAGAC 1 cut(s) 12
BstX2I RGATCY 1 cut(s) 251
BstYI RGATCY 1 cut(s) 251
Bsu36I CCTNAGG 1 cut(s) 267
BtrI CACGTC 1 cut(s) 133
BtsIMutI CAGTG 1 cut(s) 144
CfoI GCGC 1 cut(s) 294
CviAII CATG 2 cut(s) 128, 200
CviJI RGCY 9 cut(s) 91, 119, 158, 188, 219, 241, 312, 325, 386
CviKI_1 RGCY 9 cut(s) 91, 119, 158, 188, 219, 241, 312, 325, 386
DdeI CTNAG 4 cut(s) 147, 159, 267, 281
DpnI GATC 2 cut(s) 253, 409
DpnII GATC 2 cut(s) 251, 407
Eco31I GGTCTC 1 cut(s) 394
Eco57I CTGAAG 1 cut(s) 113
Eco81I CCTNAGG 1 cut(s) 267
Eco88I CYCGRG 1 cut(s) 397
FaeI CATG 2 cut(s) 131, 203
FaiI YATR 8 cut(s) 30, 87, 129, 138, 201, 390, 419, 424
FaqI GGGAC 1 cut(s) 380
FatI CATG 2 cut(s) 127, 199
GlaI GCGC 1 cut(s) 293
GsuI CTGGAG 1 cut(s) 123
HhaI GCGC 1 cut(s) 294
Hin1II CATG 2 cut(s) 131, 203
Hin6I GCGC 1 cut(s) 292
HinP1I GCGC 1 cut(s) 292
HincII GTYRAC 1 cut(s) 346
HindII GTYRAC 1 cut(s) 346
HinfI GANTC 2 cut(s) 65, 350
HpaI GTTAAC 1 cut(s) 346
HphI GGTGA 2 cut(s) 113, 380
Hpy166II GTNNAC 1 cut(s) 346
Hpy188I TCNGA 2 cut(s) 260, 355
Hpy188III TCNNGA 2 cut(s) 397, 435
Hpy8I GTNNAC 1 cut(s) 346
HpyAV CCTTC 1 cut(s) 192
HpyCH4IV ACGT 3 cut(s) 9, 132, 377
HpyCH4V TGCA 1 cut(s) 426
HpyF3I CTNAG 4 cut(s) 147, 159, 267, 281
HpySE526I ACGT 3 cut(s) 9, 132, 377
Hsp92II CATG 2 cut(s) 131, 203
HspAI GCGC 1 cut(s) 292
KspAI GTTAAC 1 cut(s) 346
Kzo9I GATC 2 cut(s) 251, 407
LmnI GCTCC 1 cut(s) 196
LpnPI CCDG 4 cut(s) 87, 279, 351, 399
MaeII ACGT 3 cut(s) 9, 132, 377
MalI GATC 2 cut(s) 253, 409
MbiI CCGCTC 1 cut(s) 306
MboI GATC 2 cut(s) 251, 407
MboII GAAGA 1 cut(s) 17
MfeI CAATTG 1 cut(s) 234
MflI RGATCY 1 cut(s) 251
MluCI AATT 3 cut(s) 163, 234, 357
MlyI GAGTC 2 cut(s) 74, 359
MnlI CCTC 4 cut(s) 142, 199, 262, 336
MroXI GAANNNNTTC 2 cut(s) 9, 276
MseI TTAA 3 cut(s) 192, 300, 345
MslI CAYNNNNRTG 1 cut(s) 126
MunI CAATTG 1 cut(s) 234
NdeII GATC 2 cut(s) 251, 407
NlaIII CATG 2 cut(s) 131, 203
NspI RCATGY 1 cut(s) 203
PdmI GAANNNNTTC 2 cut(s) 9, 276
PleI GAGTC 2 cut(s) 73, 358
PpsI GAGTC 2 cut(s) 73, 358
Psp1406I AACGTT 1 cut(s) 377
PsuI RGATCY 1 cut(s) 251
RseI CAYNNNNRTG 1 cut(s) 126
SaqAI TTAA 3 cut(s) 192, 300, 345
Sau3AI GATC 2 cut(s) 251, 407
SchI GAGTC 2 cut(s) 74, 359
SetI ASST 6 cut(s) 12, 135, 221, 243, 345, 380
SmiMI CAYNNNNRTG 1 cut(s) 126
Sse9I AATT 3 cut(s) 163, 234, 357
SsiI CCGC 1 cut(s) 306
TaiI ACGT 3 cut(s) 12, 135, 380
TaqI TCGA 2 cut(s) 63, 68
TasI AATT 3 cut(s) 163, 234, 357
Tru1I TTAA 3 cut(s) 192, 300, 345
Tru9I TTAA 3 cut(s) 192, 300, 345
TscAI CASTG 1 cut(s) 151
TspRI CASTG 1 cut(s) 151
XapI RAATTY 2 cut(s) 163, 357
XceI RCATGY 1 cut(s) 203
XmnI GAANNNNTTC 2 cut(s) 9, 276
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.